121 results on '"Testerink C"'
Search Results
2. From lab to greenhouse: molecular mechanisms of physiological control of plant growth
- Author
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Testerink, C., van der Krol, A.R., van Hoogdalem, Mark, Testerink, C., van der Krol, A.R., and van Hoogdalem, Mark
- Abstract
In horticulture, especially in the production of ornamental crops, it is important to grow compact-shaped plants. Achieving this year-round can be challenging, especially during cloudy and/or warm days because elongation of stems is stimulated under such conditions, resulting in spindly-shaped plants. Chemical plant growth retardants (PGRs) are used in horticulture to control plant elongation. However, the use of chemical PGRs needs to be replaced by more sustainable methods. A commonly used alternative method for controlling plant growth without the use of PGRs is realizing a negative day-night temperature difference (-DIF: cold days/warm nights), instead of a ‘normal’ +DIF regime (warm days/cold nights). Although -DIF is effective for controlling growth in many plant species, economic realization of -DIF is not always possible. Therefore, more alternative methods for controlling plant growth in greenhouses are needed. Aim of this thesis project was to increase our understanding of the molecular regulation of the growth response to -DIF. Additionally, we aimed to find ways to manipulate the components behind this regulation (e.g. by added light treatments) in order to enhance the effect of -DIF and/or identify alternative treatments for growing compact plants in greenhouses.To study the transcriptional responses of growth-regulating components to light and temperature cues, we used firefly-luciferase (ffLUC) reporters for promoter activity of genes involved in plant growth. To this aim LUMINATOR was developed. This custom-built system was used to semi-continuously monitor ffLUC activity in four-week-old Arabidopsis reporter plants for promoter activity of PHYTOCHROME INTERACTING FACTOR 4 (PIF4), ELONGATED HYPOCOTYL 5 (HY5) and GIGANTEA (GI) under 12h light/12h dark cycles. LUMINATOR was subsequently used to study transcriptional responses of PIF4, HY5 and GI to one hour added light at the start and end of the photoperiod. Such short light pulse treatments triggered
- Published
- 2020
3. The art of being flexible: how to escape from shade, salt and drought: The art of being flexible: how to excape from shade, salt and drought
- Author
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Pierik, Ronald, Testerink, C., Sub Plant Ecophysiology, and Plant Ecophysiology
- Subjects
fungi ,food and beverages - Abstract
Environmental stresses, such as shading of the shoot, drought, and soil salinity, threaten plant growth, yield, and survival. Plants can alleviate the impact of these stresses through various modes of phenotypic plasticity, such as shade avoidance and halotropism. Here, we review the current state of knowledge regarding the mechanisms that control plant developmental responses to shade, salt, and drought stress. We discuss plant hormones and cellular signaling pathways that control shoot branching and elongation responses to shade and root architecture modulation in response to drought and salinity. Because belowground stresses also result in aboveground changes and vice versa, we then outline how a wider palette of plant phenotypic traits is affected by the individual stresses. Consequently, we argue for a research agenda that integrates multiple plant organs, responses, and stresses. This will generate the scientific understanding needed for future crop improvement programs aiming at crops that can maintain yields under variable and suboptimal conditions.
- Published
- 2014
4. Lipid affinity beads: from identifying new lipid binding proteins to assessing their binding properties
- Author
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McLoughlin, F., Testerink, C., Munnik, T., Heilmann, I., Plant Physiology (SILS, FNWI), and Green Life Sciences
- Subjects
Lipid Â-binding ,Binding properties ,food and beverages ,Plasma protein binding ,Biology ,Phosphatidic acid target proteins ,Microsphere ,Phospholipid signaling ,Membrane protein ,Biochemistry ,In vivo ,Phosphatidic acid beads ,Lipid binding ,Phosphatidic acid ,lipids (amino acids, peptides, and proteins) - Abstract
Lipid affinity beads can be used to identify novel proteins with lipid binding capacity or to determine binding prerequisites of known lipid-binding proteins. Here we describe several applications for which this tool can be used and which considerations have to be taken into account. In addition to a precise protocol, several suggestions are made for experimental setups to facilitate identification of in vivo lipid binding targets.
- Published
- 2013
5. Liposome-Binding Assays to Assess Specificity and Affinity of Phospholipid–Protein Interactions
- Author
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Julkowska, M.M., Rankenberg, J.M., Testerink, C., Munnik, T., Heilmann, I., Plant Physiology (SILS, FNWI), and Green Life Sciences
- Subjects
Liposome ,Liposome assay ,medicine.diagnostic_test ,Chemistry ,Vesicle ,Phospholipid ,Plasma protein binding ,Protein–protein interaction ,chemistry.chemical_compound ,Binding affinity ,Phospholipid signaling ,Protein structure ,Western blot ,Biochemistry ,Phospholipid binding ,Phosphatidic acid ,medicine ,Large unilamellar vesicle (LUV) ,lipids (amino acids, peptides, and proteins) ,Centrifugation - Abstract
Protein-lipid interactions play an important role in cellular protein relocation, activation and signal transduction. The liposome-binding assay is a simple and inexpensive method to examine protein-lipid binding in vitro. The phospholipids used for liposome production are dried and hydrated. Subsequent extrusion of the phospholipid mixture ensures the production of large unilamellar vesicles (LUV) filled with raffinose. Those LUVs can be easily separated from the aqueous solution by centrifugation. By incubating a protein of interest with the LUVs and subsequent centrifugation steps, the bound protein fraction can be determined using Western Blot or Coomassie staining. This technique enables analysis of protein-lipid binding affinity and specificity.
- Published
- 2013
6. Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
- Author
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Gonorazky, G., Laxalt, A.M., Dekker, H.L., Rep, M., Munnik, T., Testerink, C., de la Canal, L., Plant Physiology (SILS, FNWI), Green Life Sciences, Molecular Plant Pathology (SILS, FNWI), and Mass Spectrometry of Biomacromolecules (SILS, FNWI)
- Subjects
fungi ,food and beverages ,Bioquímica y Biología Molecular ,Phosphoinositide ,Extracellular medium ,Ciencias Biológicas ,Apoplast ,Phosphatidylinositol 4-phosphate ,Phosphatidic acid ,Laboratorium voor Plantenfysiologie ,Lipid signalling ,Laboratory of Plant Physiology ,CIENCIAS NATURALES Y EXACTAS ,Lysophospholipid - Abstract
We have recently detected phosphatidylinositol-4-phosphate (PI4P) in the extracellular medium of tomato cell suspensions. Extracellular PI4P was shown to trigger the activation of defence responses induced by the fungal elicitor xylanase. In this study, by applying a differential centrifugation technique, we found that extracellular PI4P is associated with fractions composed of diverse phospholipids and proteins, which were pelleted from the extracellular medium of tomato cell suspensions grown under basal conditions. Using mass spectrometry, we identified the proteins present in these pelleted fractions. Most of these proteins have previously been characterised as having a role in defence responses. Next, we evaluated whether PI4P could also be detected in an entire plant system. For this, apoplastic fluids of tomato plants grown under basal conditions were analysed using a lipid overlay assay. Interestingly, PI4P could be detected in intercellular fluids obtained from tomato leaflets and xylem sap of tomato plants. By employing electrospray ionisation tandem mass spectrometry (ESI-MS/MS), other phospholipids were also found in intercellular fluids of tomato plants. These had a markedly different profile from the phospholipid pattern identified in entire leaflets. Based on these results, the potential role of extracellular phospholipids in plant intercellular communication is discussed. Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Dekker, H. L.. University Of Amsterdam; Países Bajos Fil: Rep, M.. University Of Amsterdam; Países Bajos Fil: Munnik, T.. University Of Amsterdam; Países Bajos Fil: Testerink, C.. University Of Amsterdam; Países Bajos Fil: de la Canal, L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
- Published
- 2012
7. Phosphatidic acid: An electrostatic/ hydrogen-bond switch?
- Author
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Kooijman, E.E., Testerink, C., Munnik, T., and Plant Physiology (SILS, FNWI)
- Subjects
Cell signaling ,chemistry.chemical_compound ,Hydrogen bond ,Chemistry ,Stereochemistry ,Ionization ,Life Science ,Phosphatidic acid ,Lipid bilayer ,Bioactive lipid - Abstract
Phosphatidic acid (PA) has been shown to be an important bioactive lipid that is specifically recognized by various proteins. As such, it plays a crucial role in cellular signaling in all eukaryotes. An important determinant for PA's role in its diverse functions is its anionic headgroup that resides very close to the hydrophobic interior of the lipid bilayer. In this chapter, we describe a new model, the electrostatic/ hydrogen-bond switch that describes PA's ionization properties and its specific interaction with proteins. Furthermore, we will allude to the broader implications of the model for all phosphomonoester moieties found in biological compounds. Recent data in support of the model, as well as biological predictions arising from it, are also discussed.
- Published
- 2010
8. The art of being flexible: how to escape from shade, salt and drought: The art of being flexible: how to excape from shade, salt and drought
- Author
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Sub Plant Ecophysiology, Plant Ecophysiology, Pierik, Ronald, Testerink, C., Sub Plant Ecophysiology, Plant Ecophysiology, Pierik, Ronald, and Testerink, C.
- Published
- 2014
9. zoek naar een gen voor zouttolerantie
- Author
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Testerink, C. and Testerink, C.
- Abstract
De meeste planten houden niet van zout. Handig dus dat nu uit onderzoek blijkt dat planten de groeirichting van hun wortels kunnen aanpassen in de buurt van zout. Dat is wel bijzonder omdat de wortels zich normaal gesproken laten leiden door de zwaartekracht, en dus naar beneden groeien. Biologen weten nu hoe dit zoutmijdende proces op celniveau werkt. Nu willen ze nog de genen opsporen die een plant op deze manier helpen in een zout milieu te overleven. “De primaire reactie op droogte is hetzelfde als de reactie van de plant op zout omdat er in beide gevallen sprake is van een osmotische reactie”, vertelt Christa Testerink, universitair docent en onderzoekster verbonden aan het Swammerdam Instituut voor Levenswetenschappen aan de UvA. Bij osmose verplaatst water zich door de celwand heen om de zoutconcentraties aan beide zijden van de membraan in balans te brengen.
- Published
- 2014
10. Reassessing the role of phospholipase D in the Arabidopsis wounding response
- Author
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Bargmann, B.O.R., Laxalt, A.M., van de Riet, B.P., Testerink, C., Merquiol, E., Mosblech, A., Leon Reyes, H.A., Pieterse, C.M.J., Haring, M.A., Heilmann, I., Bartels, D., Munnik, T., Ecology and Biodiversity, Plant Microbe Interactions, Sub Ecology and Biodiversity, Sub Plant-Microbe Interactions, Plant Physiology (SILS, FNWI), Ecology and Biodiversity, Plant Microbe Interactions, Sub Ecology and Biodiversity, and Sub Plant-Microbe Interactions
- Subjects
0106 biological sciences ,Physiology ,Mutant ,Arabidopsis ,Plant Science ,Phospholipase ,01 natural sciences ,Gene Knockout Techniques ,chemistry.chemical_compound ,wound response ,Solanum lycopersicum ,Gene Expression Regulation, Plant ,Phosphatidic acid ,Taverne ,phospholipase D ,Arabidopsis thaliana ,signalling ,phospholipase ,Cells, Cultured ,Regulation of gene expression ,0303 health sciences ,biology ,integumentary system ,Jasmonic acid ,Cell biology ,Biochemistry ,Larva ,International ,lipids (amino acids, peptides, and proteins) ,PLD ,Butterflies ,Biologie ,CIENCIAS NATURALES Y EXACTAS ,Phosphatidic Acids ,Cyclopentanes ,defense signaling ,Ciencias Biológicas ,03 medical and health sciences ,Biología Celular, Microbiología ,plant defense ,Phospholipase D ,Animals ,Oxylipins ,030304 developmental biology ,Arabidopsis Proteins ,wounding ,biology.organism_classification ,enzymes and coenzymes (carbohydrates) ,chemistry ,Protein Kinases ,010606 plant biology & botany - Abstract
Plants respond to wounding by means of a multitude of reactions, with the purpose of stifling herbivore assault. Phospholipase D (PLD) has previously been implicated in the wounding response. Arabidopsis (Arabidopsis thaliana) AtPLDa1 has been proposed to be activated in intact cells, and the phosphatidic acid (PA) it produces to serve as a precursor for jasmonic acid (JA) synthesis and to be required for wounding-induced gene expression. Independently, PLD activity has been reported to have a bearing on wounding-induced MAPK activation. However, which PLD isoforms are activated, where this activity takes place (in the wounded or non-wounded cells) and what exactly the consequences are is a question that has not been comprehensively addressed. Here, we show that PLD activity during the wounding response is restricted to the ruptured cells using 32Pi-labelled phospholipid analyses of Arabidopsis pld knock-out mutants and PLD-silenced tomato cell-suspension cultures. plda1 knock-out lines have reduced wounding-induced PA production, and the remainder is completely eliminated in a plda1/d double knock-out line. Surprisingly, wounding-induced protein kinase activation, AtLOX2 gene expression and JA biosynthesis were not affected in these knock-out lines. Moreover, larvae of the Cabbage White butterfly (Pieris rapae) grew equally well on wild-type and the pld knock-out mutants. Fil: Bargmann, Bastiaan O. R.. University of Amsterdam; Países Bajos Fil: Laxalt, Ana Maria. University of Amsterdam; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Riet, Bas Ter. University of Amsterdam; Países Bajos Fil: Testerink, Christa. University of Amsterdam; Países Bajos Fil: Merquiol, Emmanuelle. Vrije Universiteit Amsterdam; Países Bajos Fil: Mosblech, Alina. Georg August University; Alemania Fil: Leon Reyes, Antonio. Utrecht University; Países Bajos Fil: Pieterse, Corné M. J.. Utrecht University; Países Bajos Fil: Haring, Michel A.. University of Amsterdam; Países Bajos Fil: Heilmann, Ingo. Georg August University; Alemania Fil: Bartels, Dorothea. Vrije Universiteit Amsterdam; Países Bajos Fil: Munnik, Teun. Universitat Bonn; Alemania
- Published
- 2009
11. An electrostatic/hydrogen bond switch as the basis for the specific interaction of phosphatidic acid with proteins
- Author
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Kooijman, E.E., Tieleman, D.P., Testerink, C., Munnik, T., Rijkers, D.T.S., Burger, K.N.J., de Kruijff, B., Biochemie van Membranen, Chemical Biology & Organic Chemistry, SYNTHESE, Dep Scheikunde, Dep Farmaceutische wetenschappen, and Dep Biologie
- Abstract
Phosphatidic acid (PA) is a minor but important phospholipid that, through specific interactions with proteins, plays a central role in several key cellular processes. The simple yet unique structure of PA, carrying just a phosphomonoester head group, suggests an important role for interactions with the positively charged essential residues in these proteins. We analyzed by solid-state magic angle spinning 31P NMR and molecular dynamics simulations the interaction of low concentrations of PA in model membranes with positively charged side chains of membrane-interacting peptides. Surprisingly, lysine and arginine residues increase the charge of PA, predominantly by forming hydrogen bonds with the phosphate of PA, thereby stabilizing the protein-lipid interaction. Our results demonstrate that this electrostatic/hydrogen bond switch turns the phosphate of PA into an effective and preferred docking site for lysine and arginine residues. In combination with the special packing properties of PA, PA may well be nature’s preferred membrane lipid for interfacial insertion of positively charged membrane protein domains.
- Published
- 2007
12. Plant response to stress: phosphatidic acid as a second messenger
- Author
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Testerink, C., Munnik, T., Goodman, R.M., and Plant Physiology (SILS, FNWI)
- Published
- 2004
13. Aspects of ABA and fusicoccin signal transduction in barley grains: I. Effect of fusicoccin on ABA-induced gene expression in embryo and aleurone; II. Spatial and temporal expression of 14-3-3 proteins in dormant embryos.
- Author
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Testerink, C., primary, Meulen, R. M. van der, additional, and Wang, M, additional
- Full Text
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14. Post-translational modification of barley 14-3-3A is isoform-specific and involves removal of the hypervariable C-terminus
- Author
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Testerink, C., Zeijl, M.J. van, Drumm, K., Palmgren, M.G., Collinge, D.B., Kijne, J.W., Wang, M., TNO Voeding, and Plant Physiology (SILS, FNWI)
- Subjects
Biology - Published
- 2002
15. Subcellular differences in post-translational modification of barley 14-3-3 proteins
- Author
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Zeijl, M.J. van, Testerink, C., Kijne, J.W., and Wang, M.
- Subjects
Tyrosine 3-Monooxygenase ,Blotting, Western ,protein antibody ,Germination ,Antibodies ,protein modification ,Western blotting ,Hordeum vulgare subsp. vulgare ,14-3-3 Protein ,Hordeum distichum ,Cell compartment ,Protein Isoforms ,controlled study ,Trypsin ,vegetable protein ,microsome ,protein expression ,Plant Proteins ,carboxy terminal sequence ,cell nucleus ,barley ,protein processing ,Proteins ,Hordeum ,cell fractionation ,priority journal ,14-3-3 Proteins ,electrophoresis ,Seeds ,isoprotein ,protein degradation ,Electrophoresis, Polyacrylamide Gel ,Staphylococcus phage 3A ,Post-translational modification ,cytosol ,Protein Processing, Post-Translational ,Subcellular Fractions - Abstract
Expression and post-translational modification of barley 14-3-3 isoforms, 14-3-3A, 14-3-3B and 14-3-3C, were investigated using isoform-specific antibodies. Although all three isoforms were shown to be present in the cytosolic, the nuclear and the microsomal cell fractions, differences in post-translational modification were identified for the different cell fractions. Germination-related modifications of 14-3-3 proteins were observed in the cytosol and the microsomal fraction, but not in the nucleus. In vitro proteolytic cleavage of 14-3-3 proteins using trypsin suggests that for 14-3-3A this change was caused by proteolytic cleavage of the unconserved C-terminal region. Copyright (C) 2000 Federation of European Biochemical Societies. Chemicals/CAS: 14-3-3 Proteins; Antibodies; Plant Proteins; Protein Isoforms; Proteins; Trypsin, EC 3.4.21.4; Tyrosine 3-Monooxygenase, EC 1.14.16.2
- Published
- 2000
16. 14-3-3 proteins interact with a 13-lipoxygenase, but not with a 9-lipoxygenase
- Author
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Holtman, W.L., Roberts, M.R., Oppedijk, B.J., Testerink, C., Zeijl, M.J. van, and Wang, M.
- Subjects
Interaction ,Tyrosine 3-Monooxygenase ,13 lipoxygenase ,Lipoxygenase ,enzyme purification ,immunoprecipitation ,Western blotting ,Hordeum vulgare subsp. vulgare ,Escherichia coli ,Immunosorbent Techniques ,enzyme substrate complex ,Chemistry, Physical ,food and beverages ,barley ,Antibodies, Monoclonal ,Proteins ,nucleotide sequence ,Hordeum ,Surface Plasmon Resonance ,protein family ,Recombinant Proteins ,unclassified drug ,protein phosphorylation ,Isoenzymes ,germination ,14-3-3 Proteins ,9 lipoxygenase - Abstract
Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development. Copyright (C) 2000 Federation of European Biochemical Societies. Molecular Sequence Numbers: GENBANK: L35931, L37358, X62388, X93170, Y14200; Chemicals/CAS: 13-lipoxygenase, EC 1.13.11.-; 14-3-3 Proteins; Antibodies, Monoclonal; Isoenzymes; Lipoxygenase, EC 1.13.11.12; Proteins; Recombinant Proteins; Tyrosine 3-Monooxygenase, EC 1.14.16.2
- Published
- 2000
17. Sequences surrounding the transcription initiation site of the Arabidopsis enoyl-acyl carrier protein reductase gene control seed expression in transgenic tobacco
- Author
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de Boer, G.J., Testerink, C, Pielage, J.A., Nijkamp, H.J.J., Stuitje, A.R., and Developmental Genetics
- Subjects
SDG 3 - Good Health and Well-being ,fungi ,food and beverages - Abstract
The NADH-specific enoyl-acyl carrier protein (ACP) reductase, which catalyses the last reducing step during the fatty acid biosynthesis cycle, is encoded in Arabidopsis thaliana encoded by a single housekeeping gene (ENRA) which is differentially expressed during plant development. To identify elements involved in its tissue-specific transcriptional control, a fragment comprising the 1470 bp region directly upstream of the ATG start codon of the ENR-A gene was fused to the uidA (GUS) reporter gene and analysed in transgenic Nicotiana tabacum plants. GUS activity found during development of the transgenic plants was similar to endogenous ENR protein levels found in both tobacco and Arabidopsis plants, except for developing flowers. In floral tissue the promoter fragment showed very little activity in contrast to the relatively high level of endogenous ENR expression. Successive deletions from the 5' and 3' regions of the promoter fragment revealed the presence of at least three elements which control GUS expression in different stages of development in the transgenic tobacco plants. First, expression in young developing leaves required both the presence of sequences between -329 to -201 relative to the transcription start and part of the untranslated leader comprising the first intron. Second, root-specific GUS expression was still observed after deletion of the 5'-upstream sequences up to 19 bp of the transcription initiation site. Further, the additional removal of the intron from the untranslated leader increased root-specific expression by ca. 4- to 5-fold. Third, high expression in seeds was still observed with the minimal upstream promoter segment of 19 bp. This seed expression level was found to be independent of the presence or absence of the intron in the untranslated leader. Finally, 3' deletion of the leader sequence up to 17 bp of the transcription start greatly impaired GUS activity during all stages of plant development, suggesting that the deleted sequence of the leader either functions as an enhancer for transcription initiation or stabilizes the mRNA.
- Published
- 1999
18. Reassessing the role of phospholipase D in the Arabidopsis wounding response.
- Author
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Ecology and Biodiversity, Plant Microbe Interactions, Sub Ecology and Biodiversity, Sub Plant-Microbe Interactions, Bargmann, B.O.R., Laxalt, A.M., van de Riet, B.P., Testerink, C., Merquiol, E., Mosblech, A., Leon Reyes, H.A., Pieterse, C.M.J., Haring, M.A., Heilmann, I., Bartels, D., Munnik, T., Ecology and Biodiversity, Plant Microbe Interactions, Sub Ecology and Biodiversity, Sub Plant-Microbe Interactions, Bargmann, B.O.R., Laxalt, A.M., van de Riet, B.P., Testerink, C., Merquiol, E., Mosblech, A., Leon Reyes, H.A., Pieterse, C.M.J., Haring, M.A., Heilmann, I., Bartels, D., and Munnik, T.
- Published
- 2009
19. Identification of novel candidate phosphatidic acid-binding proteins involved in the salt-stress response of Arabidopsis thaliana roots
- Author
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Mcloughlin, F., primary, Arisz, S. A., additional, Dekker, H. L., additional, Kramer, G., additional, de Koster, C. G., additional, Haring, M. A., additional, Munnik, T., additional, and Testerink, C., additional
- Published
- 2013
- Full Text
- View/download PDF
20. An electrostatic/hydrogen bond switch as the basis for the specific interaction of phosphatidic acid with proteins
- Author
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Biochemie van Membranen, Chemical Biology & Organic Chemistry, SYNTHESE, Dep Scheikunde, Dep Farmaceutische wetenschappen, Dep Biologie, Kooijman, E.E., Tieleman, D.P., Testerink, C., Munnik, T., Rijkers, D.T.S., Burger, K.N.J., de Kruijff, B., Biochemie van Membranen, Chemical Biology & Organic Chemistry, SYNTHESE, Dep Scheikunde, Dep Farmaceutische wetenschappen, Dep Biologie, Kooijman, E.E., Tieleman, D.P., Testerink, C., Munnik, T., Rijkers, D.T.S., Burger, K.N.J., and de Kruijff, B.
- Published
- 2007
21. Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
- Author
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Gonorazky, G., primary, Laxalt, A. M., additional, Dekker, H. L., additional, Rep, M., additional, Munnik, T., additional, Testerink, C., additional, and de la Canal, L., additional
- Published
- 2011
- Full Text
- View/download PDF
22. Molecular, cellular, and physiological responses to phosphatidic acid formation in plants
- Author
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Testerink, C., primary and Munnik, T., additional
- Published
- 2011
- Full Text
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23. Phospholipid Signaling Responses in Salt-Stressed Rice Leaves
- Author
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Darwish, E., primary, Testerink, C., additional, Khalil, M., additional, El-Shihy, O., additional, and Munnik, T., additional
- Published
- 2009
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24. Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
- Author
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Bargmann, B. O. R., primary, Laxalt, A. M., additional, Riet, B. t., additional, van Schooten, B., additional, Merquiol, E., additional, Testerink, C., additional, Haring, M. A., additional, Bartels, D., additional, and Munnik, T., additional
- Published
- 2008
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25. Phosphatidic acid binds to and inhibits the activity of Arabidopsis CTR1
- Author
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Testerink, C., primary, Larsen, P. B., additional, van der Does, D., additional, van Himbergen, J. A.J., additional, and Munnik, T., additional
- Published
- 2007
- Full Text
- View/download PDF
26. Characterisation of a major QTL for sodium accumulation in tomato grown in high salinity.
- Author
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Héreil, A., Guillaume, M., Duboscq, R., Carretero, Y., Pelpoir, E., Bitton, F., Giraud, C., Karlova, R., Testerink, C., Stevens, R., and Causse, M.
- Subjects
- *
LOCUS (Genetics) , *SOIL salinity , *BIOACCUMULATION , *GENETIC variation , *GENE expression , *TOMATOES - Abstract
Soil salinity is a serious concern for tomato culture, affecting both yield and quality parameters. Although some genes involved in tomato salt tolerance have been identified, their genetic diversity has been rarely studied. In the present study, we assessed salt tolerance‐related traits at juvenile and adult stages in a large core collection and identified salt tolerance quantitative trait loci (QTLs) by genome‐wide association study (GWAS). The results suggested that a major QTL is involved in leaf sodium accumulation at both physiological stages. We were able to identify the underlying candidate gene, coding for a well‐known sodium transporter, called
SlHKT1.2 . We showed that an eQTL for the expression of this gene in roots colocalized with the above ground sodium content QTL. A polymorphism putatively responsible for its variation was identified in the gene promoter. Finally, to extend the applicability of these results, we carried out the same analysis on a test‐cross panel composed of the core collection crossed with a distant line. The results indicated that the identified QTL retained its functional impact even in a hybrid genetic context: this paves the way for its use in breeding programs aimed at improving salinity tolerance in tomato cultivars. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
27. 14-3-3 proteins interact with a 13-lipoxygenase, but not with a 9-lipoxygenase
- Author
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Holtman, W. L., Roberts, M. R., Oppedijk, B. J., Testerink, C., Zeijl, M. J. van, and Wang, M.
- Published
- 2000
- Full Text
- View/download PDF
28. Subcellular differences in post-translational modification of barley 14-3-3 proteins
- Author
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Zeijl, M. J. van, Testerink, C., Kijne, J. W., and Wang, M.
- Published
- 2000
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29. Differences in spatial expression between 14-3-3 isoforms in germinating barley embryos.
- Author
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Testerink, C, van der Meulen, R M, Oppedijk, B J, de Boer, A H, Heimovaara-Dijkstra, S, Kijne, J W, and Wang, M
- Abstract
The family of 14-3-3 proteins is ubiquitous in eukaryotes and has been shown to exert an array of functions. We were interested in the possible role of 14-3-3 proteins in seed germination. Therefore, we studied the expression of 14-3-3 mRNA and protein in barley (Hordeum distichum L.) embryos during germination. With the use of specific cDNA probes and antibodies, we could detect individual expression of three 14-3-3 isoforms, 14-3-3A, 14-3-3B, and 14-3-3C. Each homolog was found to be expressed in barley embryos. Whereas protein levels of all three isoforms were constant during germination, mRNA expression was found to be induced upon imbibition of the grains. The induction of 14-3-3A gene expression during germination was different from that of 14-3-3B and 14-3-3C. In situ immunolocalization analysis showed similar spatial expression for 14-3-3A and 14-3-3B, while 14-3-3C expression was markedly different. Whereas 14-3-3A and 14-3-3B were expressed throughout the embryo, 14-3-3C expression was tissue specific, with the strongest expression observed in the scutellum and the L2 layer of the shoot apical meristem. These results show that 14-3-3 homologs are differently regulated in barley embryos, and provide a first step in acquiring more knowledge about the role of 14-3-3 proteins in the germination process.
- Published
- 1999
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30. Erratum: Identification of novel candidate phosphatidic acid-binding proteins involved in the salt-stress response of Arabidopsis thaliana roots (Biochemical Journal (2013) 450 (573-581))
- Author
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Mcloughlin, F., Arisz, S. A., Dekker, H. L., Gertjan Kramer, Koster, C. G., Haring, M. A., Munnik, T., and Testerink, C.
31. Arabinosylation of cell wall extensin is required for the directional response to salinity in roots.
- Author
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Zou Y, Gigli-Bisceglia N, van Zelm E, Kokkinopoulou P, Julkowska MM, Besten M, Nguyen TP, Li H, Lamers J, de Zeeuw T, Dongus JA, Zeng Y, Cheng Y, Koevoets IT, Jørgensen B, Giesbers M, Vroom J, Ketelaar T, Petersen BL, Engelsdorf T, Sprakel J, Zhang Y, and Testerink C
- Subjects
- Glycoproteins metabolism, Glycoproteins genetics, Plant Proteins metabolism, Plant Proteins genetics, Gravitropism, Arabinose metabolism, Sodium Chloride pharmacology, Gene Expression Regulation, Plant drug effects, Glycosylation, Cell Wall metabolism, Plant Roots metabolism, Plant Roots growth & development, Plant Roots genetics, Plant Roots drug effects, Arabidopsis genetics, Arabidopsis metabolism, Arabidopsis drug effects, Arabidopsis Proteins metabolism, Arabidopsis Proteins genetics, Salinity
- Abstract
Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na+ ions, but not by osmotic stress. Next, we measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions and discovered a genetic locus, encoding the cell wall-modifying enzyme EXTENSIN ARABINOSE DEFICIENT TRANSFERASE (ExAD) that is associated with root bending in the presence of NaCl (hereafter salt). Extensins are a class of structural cell wall glycoproteins known as hydroxyproline (Hyp)-rich glycoproteins, which are posttranslationally modified by O-glycosylation, mostly involving Hyp-arabinosylation. We show that salt-induced ExAD-dependent Hyp-arabinosylation influences root bending responses and cell wall thickness. Roots of exad1 mutant seedlings, which lack Hyp-arabinosylation of extensin, displayed increased thickness of root epidermal cell walls and greater cell wall porosity. They also showed altered gravitropic root bending in salt conditions and a reduced salt-avoidance response. Our results suggest that extensin modification via Hyp-arabinosylation is a unique salt-specific cellular process required for the directional response of roots exposed to salinity., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
- Published
- 2024
- Full Text
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32. The bryophyte rhizoid-sphere microbiome responds to water deficit.
- Author
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Berdaguer R, van der Wielen N, Lorenzo ZC, Testerink C, and Karlova R
- Abstract
The roots of vascular plants are colonised by a multitude of microbes, which play an important role in plant health and stress resilience. Drought stress in particular is devastating for crop yield and causes major shifts in the rhizosphere microbial communities. However, the microbiome associated to the rhizoids (hereafter termed rhizoid-sphere) of the nonvascular bryophytes remains largely unexplored. Here, we use amplicon sequencing to explore the rhizoid-sphere microbiome of three bryophyte species under drought and well-watered conditions. Comparing rhizoid-sphere microbial communities associated with the two liverworts Marchantia polymorpha and Marchantia paleacea and the moss Physcomitrium patens showed characteristic differences in composition between host species and both conserved and unique changes under drought. At phylum level, these changes were similar to changes in the rhizosphere of angiosperms under drought. Furthermore, we observed strong differences in rhizoid-sphere colonisation between bryophyte species for taxa known for nitrogen fixation and plant growth promotion. Interestingly, M. polymorpha prioritised the growth of belowground organs under osmotic stress, as is the case for angiosperms under drought. Taken together, our results show interesting parallels between bryophytes and angiosperms in the relation with their rhizo(id-)sphere, suggesting evolutionary conservation among land plants in their response to drought stress., (© 2024 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.)
- Published
- 2024
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33. Root branching under high salinity requires auxin-independent modulation of LATERAL ORGAN BOUNDARY DOMAIN 16 function.
- Author
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Zhang Y, Li Y, de Zeeuw T, Duijts K, Kawa D, Lamers J, Munzert KS, Li H, Zou Y, Meyer AJ, Yan J, Verstappen F, Wang Y, Gijsberts T, Wang J, Gigli-Bisceglia N, Engelsdorf T, van Dijk ADJ, and Testerink C
- Subjects
- Indoleacetic Acids metabolism, Salinity, Plant Roots metabolism, Gene Expression Regulation, Plant, Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism
- Abstract
Salinity stress constrains lateral root (LR) growth and severely affects plant growth. Auxin signaling regulates LR formation, but the molecular mechanism by which salinity affects root auxin signaling and whether salt induces other pathways that regulate LR development remains unknown. In Arabidopsis thaliana, the auxin-regulated transcription factor LATERAL ORGAN BOUNDARY DOMAIN 16 (LBD16) is an essential player in LR development under control conditions. Here, we show that under high-salt conditions, an alternative pathway regulates LBD16 expression. Salt represses auxin signaling but, in parallel, activates ZINC FINGER OF ARABIDOPSIS THALIANA 6 (ZAT6), a transcriptional activator of LBD16. ZAT6 activates LBD16 expression, thus contributing to downstream cell wall remodeling and promoting LR development under high-salt conditions. Our study thus shows that the integration of auxin-dependent repressive and salt-activated auxin-independent pathways converging on LBD16 modulates root branching under high-salt conditions., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
- Published
- 2024
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34. Subtilase-mediated biogenesis of the expanded family of SERINE RICH ENDOGENOUS PEPTIDES.
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Yang H, Kim X, Skłenar J, Aubourg S, Sancho-Andrés G, Stahl E, Guillou MC, Gigli-Bisceglia N, Tran Van Canh L, Bender KW, Stintzi A, Reymond P, Sánchez-Rodríguez C, Testerink C, Renou JP, Menke FLH, Schaller A, Rhodes J, and Zipfel C
- Subjects
- Serine, Peptides, Protein Kinases genetics, Receptors, Cell Surface genetics, Arabidopsis Proteins genetics, Arabidopsis physiology, Brassicaceae
- Abstract
Plant signalling peptides are typically released from larger precursors by proteolytic cleavage to regulate plant growth, development and stress responses. Recent studies reported the characterization of a divergent family of Brassicaceae-specific peptides, SERINE RICH ENDOGENOUS PEPTIDES (SCOOPs), and their perception by the leucine-rich repeat receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2). Here, we reveal that the SCOOP family is highly expanded, containing at least 50 members in the Columbia-0 reference Arabidopsis thaliana genome. Notably, perception of these peptides is strictly MIK2-dependent. How bioactive SCOOP peptides are produced, and to what extent their perception is responsible for the multiple physiological roles associated with MIK2 are currently unclear. Using N-terminomics, we validate the N-terminal cleavage site of representative PROSCOOPs. The cleavage sites are determined by conserved motifs upstream of the minimal SCOOP bioactive epitope. We identified subtilases necessary and sufficient to process PROSCOOP peptides at conserved cleavage motifs. Mutation of these subtilases, or their recognition motifs, suppressed PROSCOOP cleavage and associated overexpression phenotypes. Furthermore, we show that higher-order mutants of these subtilases show phenotypes reminiscent of mik2 null mutant plants, consistent with impaired PROSCOOP biogenesis, and demonstrating biological relevance of SCOOP perception by MIK2. Together, this work provides insights into the molecular mechanisms underlying the functions of the recently identified SCOOP peptides and their receptor MIK2., (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
- Published
- 2023
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35. Balancing growth amidst salt stress - lifestyle perspectives from the extremophyte model Schrenkiella parvula.
- Author
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Tran KN, Pantha P, Wang G, Kumar N, Wijesinghege C, Oh DH, Wimalagunasekara S, Duppen N, Li H, Hong H, Johnson JC, Kelt R, Matherne MG, Nguyen TT, Garcia JR, Clement A, Tran D, Crain C, Adhikari P, Zhang Y, Foroozani M, Sessa G, Larkin JC, Smith AP, Longstreth D, Finnegan P, Testerink C, Barak S, and Dassanayake M
- Subjects
- Flowers, Salt Stress, Stress, Physiological, Water, Brassicaceae physiology, Arabidopsis physiology
- Abstract
Schrenkiella parvula, a leading extremophyte model in Brassicaceae, can grow and complete its lifecycle under multiple environmental stresses, including high salinity. Yet, the key physiological and structural traits underlying its stress-adapted lifestyle are unknown along with trade-offs when surviving salt stress at the expense of growth and reproduction. We aimed to identify the influential adaptive trait responses that lead to stress-resilient and uncompromised growth across developmental stages when treated with salt at levels known to inhibit growth in Arabidopsis and most crops. Its resilient growth was promoted by traits that synergistically allowed primary root growth in seedlings, the expansion of xylem vessels across the root-shoot continuum, and a high capacity to maintain tissue water levels by developing thicker succulent leaves while enabling photosynthesis during salt stress. A successful transition from vegetative to reproductive phase was initiated by salt-induced early flowering, resulting in viable seeds. Self-fertilization in salt-induced early flowering was dependent upon filament elongation in flowers otherwise aborted in the absence of salt during comparable plant ages. The maintenance of leaf water status promoting growth, and early flowering to ensure reproductive success in a changing environment, were among the most influential traits that contributed to the extremophytic lifestyle of S. parvula., (© 2023 Society for Experimental Biology and John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
36. Natural variation in salt-induced root growth phases and their contribution to root architecture plasticity.
- Author
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van Zelm E, Bugallo-Alfageme S, Behrouzi P, Meyer AJ, Testerink C, and Gommers CMM
- Subjects
- Salt Stress, Phenotype, Plant Roots, Arabidopsis physiology
- Abstract
The root system architecture of a plant changes during salt stress exposure. Different accessions of Arabidopsis thaliana have adopted different strategies in remodelling their root architecture during salt stress. Salt induces a multiphase growth response in roots, consisting of a stop phase, quiescent phase, recovery phase and eventually a new level of homoeostasis. We explored natural variation in the length of and growth rate during these phases in both main and lateral roots and find that some accessions lack the quiescent phase. Using mathematical models and correlation-based network, allowed us to correlate dynamic traits to overall root architecture and discover that both the main root growth rate during homoeostasis and lateral root appearance are the strongest determinants of overall root architecture. In addition, this approach revealed a trade-off between investing in main or lateral root length during salt stress. By studying natural variation in high-resolution temporal root growth using mathematical modelling, we gained new insights in the interactions between dynamic root growth traits and we identified key traits that modulate overall root architecture during salt stress., (© 2023 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
37. Effective root responses to salinity stress include maintained cell expansion and carbon allocation.
- Author
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Li H, Duijts K, Pasini C, van Santen JE, Lamers J, de Zeeuw T, Verstappen F, Wang N, Zeeman SC, Santelia D, Zhang Y, and Testerink C
- Subjects
- Carbon, Salt-Tolerant Plants, Salt Tolerance, Salinity, Stress, Physiological genetics, Plant Roots genetics, Gene Expression Regulation, Plant, Arabidopsis genetics, Brassicaceae genetics
- Abstract
Acclimation of root growth is vital for plants to survive salt stress. Halophytes are great examples of plants that thrive even under severe salinity, but their salt tolerance mechanisms, especially those mediated by root responses, are still largely unknown. We compared root growth responses of the halophyte Schrenkiella parvula with its glycophytic relative species Arabidopsis thaliana under salt stress and performed transcriptomic analysis of S. parvula roots to identify possible gene regulatory networks underlying their physiological responses. Schrenkiella parvula roots do not avoid salt and experience less growth inhibition under salt stress. Salt-induced abscisic acid levels were higher in S. parvula roots compared with Arabidopsis. Root transcriptomic analysis of S. parvula revealed the induction of sugar transporters and genes regulating cell expansion and suberization under salt stress.
14 C-labeled carbon partitioning analyses showed that S. parvula continued allocating carbon to roots from shoots under salt stress while carbon barely allocated to Arabidopsis roots. Further physiological investigation revealed that S. parvula roots maintained root cell expansion and enhanced suberization under severe salt stress. In summary, roots of S. parvula deploy multiple physiological and developmental adjustments under salt stress to maintain growth, providing new avenues to improve salt tolerance of plants using root-specific strategies., (© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.)- Published
- 2023
- Full Text
- View/download PDF
38. Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator.
- Author
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Wang P, Siao W, Zhao X, Arora D, Wang R, Eeckhout D, Van Leene J, Kumar R, Houbaert A, De Winne N, Mylle E, Vandorpe M, Korver RA, Testerink C, Gevaert K, Vanneste S, De Jaeger G, Van Damme D, and Russinova E
- Subjects
- trans-Golgi Network metabolism, Golgi Apparatus metabolism, Clathrin metabolism, Arabidopsis metabolism, Arabidopsis Proteins metabolism
- Abstract
Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargoes and coated vesicle accessory proteins. As in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargoes of overlapping vesicular routes are anticipated. The plant AP complexes are part of the sorting machinery, but despite some functional information, their cargoes, accessory proteins and regulation remain largely unknown. Here, by means of various proteomics approaches, we generated the overall interactome of the five AP and the TPLATE complexes in Arabidopsis thaliana. The interactome converged on a number of hub proteins, including the thus far unknown adaptin binding-like protein, designated P34. P34 interacted with the clathrin-associated AP complexes, controlled their stability and, subsequently, influenced clathrin-mediated endocytosis and various post-Golgi trafficking routes. Altogether, the AP interactome network offers substantial resources for further discoveries of unknown endomembrane trafficking regulators in plant cells., (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
- Published
- 2023
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39. Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress.
- Author
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Verslues PE, Bailey-Serres J, Brodersen C, Buckley TN, Conti L, Christmann A, Dinneny JR, Grill E, Hayes S, Heckman RW, Hsu PK, Juenger TE, Mas P, Munnik T, Nelissen H, Sack L, Schroeder JI, Testerink C, Tyerman SD, Umezawa T, and Wigge PA
- Subjects
- Plant Transpiration physiology, Plants metabolism, Water metabolism, Carbon Dioxide metabolism, Stress, Physiological, Climate Change
- Abstract
We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these questions include the need to better understand how plants detect water availability, temperature, salinity, and rising carbon dioxide (CO2) levels; how environmental signals interface with endogenous signaling and development (e.g. circadian clock and flowering time); and how this integrated signaling controls downstream responses (e.g. stomatal regulation, proline metabolism, and growth versus defense balance). The plasma membrane comes up frequently as a site of key signaling and transport events (e.g. mechanosensing and lipid-derived signaling, aquaporins). Adaptation to water extremes and rising CO2 affects hydraulic architecture and transpiration, as well as root and shoot growth and morphology, in ways not fully understood. Environmental adaptation involves tradeoffs that limit ecological distribution and crop resilience in the face of changing and increasingly unpredictable environments. Exploration of plant diversity within and among species can help us know which of these tradeoffs represent fundamental limits and which ones can be circumvented by bringing new trait combinations together. Better defining what constitutes beneficial stress resistance in different contexts and making connections between genes and phenotypes, and between laboratory and field observations, are overarching challenges., (© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
- Published
- 2023
- Full Text
- View/download PDF
40. How plant roots go with the flow.
- Author
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Testerink C and Lamers J
- Subjects
- Plant Roots, Gene Expression Regulation, Plant
- Published
- 2022
- Full Text
- View/download PDF
41. Arabidopsis root responses to salinity depend on pectin modification and cell wall sensing.
- Author
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Gigli-Bisceglia N, van Zelm E, Huo W, Lamers J, and Testerink C
- Subjects
- Cell Wall metabolism, Gene Expression Regulation, Plant, Pectins, Salinity, Arabidopsis metabolism, Arabidopsis Proteins metabolism
- Abstract
Owing to its detrimental effect on plant growth, salinity is an increasing worldwide problem for agriculture. To understand the molecular mechanisms activated in response to salt in Arabidopsis thaliana, we investigated the Catharanthus roseus receptor-like kinase 1-like family, which contains sensors that were previously shown to be involved in sensing the structural integrity of the cell walls. We found that herk1 the1-4 double mutants, lacking the function of HERKULES1 (HERK1) and combined with a gain-of-function allele of THESEUS1 (THE1), strongly respond to salt application, resulting in an intense activation of stress responses, similarly to plants lacking FERONIA (FER) function. We report that salt triggers pectin methyl esterase (PME) activation and show its requirement for the activation of several salt-dependent responses. Because chemical inhibition of PMEs alleviates these salt-induced responses, we hypothesize a model in which salt directly leads to cell wall modifications through the activation of PMEs. Responses to salt partly require the functionality of FER alone or HERK1/THE1 to attenuate salt effects, highlighting the complexity of the salt-sensing mechanisms that rely on cell wall integrity., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2022. Published by The Company of Biologists Ltd.)
- Published
- 2022
- Full Text
- View/download PDF
42. Root dynamic growth strategies in response to salinity.
- Author
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Zou Y, Zhang Y, and Testerink C
- Subjects
- Crops, Agricultural, Plant Roots metabolism, Soil, Salinity, Salt Tolerance genetics
- Abstract
Increasing soil salinization largely impacts crop yield worldwide. To deal with salinity stress, plants exhibit an array of responses, including root system architecture remodelling. Here, we review recent progress in physiological, developmental and cellular mechanisms of root growth responses to salinity. Most recent research in modulation of root branching, root tropisms, as well as in root cell wall modifications under salinity stress, is discussed in the context of the contribution of these responses to overall plant performance. We highlight the power of natural variation approaches revealing novel potential pathways responsible for differences in root salt stress responses. Together, these new findings promote our understanding of how salt shapes the root phenotype, which may provide potential avenues for engineering crops with better yield and survival in saline soils., (© 2021 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.)
- Published
- 2022
- Full Text
- View/download PDF
43. Metal halide perovskite toxicity effects on Arabidopsis thaliana plants are caused by iodide ions.
- Author
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Hutter EM, Sangster R, Testerink C, Ehrler B, and Gommers CMM
- Abstract
Highly efficient solar cells containing lead halide perovskites are expected to revolutionize sustainable energy production in the coming years. Perovskites are generally assumed to be toxic because of the lead (Pb), but experimental evidence to support this prediction is scarce. We tested the toxicity of the perovskite MAPbI
3 (MA = CH3 NH3 ) and several precursors in Arabidopsis thaliana plants. Both MAPbI3 and the precursor MAI hamper plant growth at concentrations above 5 μM. Lead-based precursors without iodide are only toxic above 500 μM. Iodine accumulation in Arabidopsis correlates with growth inhibition at much lower concentrations than lead. This reveals that perovskite toxicity at low concentrations is caused by iodide ions specifically, instead of lead. We calculate that toxicity thresholds for iodide, but not lead, are likely to be reached in soils upon perovskite leakage. This work stresses the importance to further understand and predict harmful effects of iodide-containing perovskites in the environment., Competing Interests: The authors declare no competing interests., (© 2021 The Author(s).)- Published
- 2021
- Full Text
- View/download PDF
44. Fighting salt or enemies: shared perception and signaling strategies.
- Author
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Gigli-Bisceglia N and Testerink C
- Subjects
- Gene Expression Regulation, Plant, Perception, Plants metabolism, Salinity, Salt Stress, Stress, Physiological, Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism
- Abstract
Plants react to a myriad of biotic and abiotic environmental signals through specific cellular mechanisms required for survival under stress. Although pathogen perception has been widely studied and characterized, salt stress perception and signaling remain largely elusive. Recent observations, obtained in the model plant Arabidopsis thaliana, show that perception of specific features of pathogens also allows plants to mount salt stress resilience pathways, highlighting the possibility that salt sensing and pathogen perception mechanisms partially overlap. We discuss these overlapping strategies and examine the emerging role of A. thaliana cell wall and plasma membrane components in activating both salt- and pathogen-induced responses, as part of exquisite mechanisms underlying perception of damage and danger. This knowledge helps understanding the complexity of plant responses to pathogens and salinity, leading to new hypotheses that could explain why plants evolved similar strategies to respond to these, at first sight, very different types of stimuli., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
45. Root plasticity under abiotic stress.
- Author
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Karlova R, Boer D, Hayes S, and Testerink C
- Subjects
- Agriculture, Cold Temperature, Crops, Agricultural anatomy & histology, Crops, Agricultural genetics, Crops, Agricultural growth & development, Droughts, Floods, Hot Temperature, Plant Roots anatomy & histology, Plant Roots genetics, Plant Roots growth & development, Soil, Adaptation, Physiological, Cell Plasticity, Crops, Agricultural physiology, Plant Growth Regulators metabolism, Plant Roots physiology, Stress, Physiological
- Abstract
Abiotic stresses increasingly threaten existing ecological and agricultural systems across the globe. Plant roots perceive these stresses in the soil and adapt their architecture accordingly. This review provides insights into recent discoveries showing the importance of root system architecture (RSA) and plasticity for the survival and development of plants under heat, cold, drought, salt, and flooding stress. In addition, we review the molecular regulation and hormonal pathways involved in controlling RSA plasticity, main root growth, branching and lateral root growth, root hair development, and formation of adventitious roots. Several stresses affect root anatomy by causing aerenchyma formation, lignin and suberin deposition, and Casparian strip modulation. Roots can also actively grow toward favorable soil conditions and avoid environments detrimental to their development. Recent advances in understanding the cellular mechanisms behind these different root tropisms are discussed. Understanding root plasticity will be instrumental for the development of crops that are resilient in the face of abiotic stress., (© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
- Published
- 2021
- Full Text
- View/download PDF
46. How roots and shoots communicate through stressful times.
- Author
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Li H, Testerink C, and Zhang Y
- Subjects
- Plant Shoots, Soil, Water, Plant Roots, Stress, Physiological
- Abstract
When plants face an environmental stress such as water deficit, soil salinity, high temperature, or shade, good communication between above- and belowground organs is necessary to coordinate growth and development. Various signals including hormones, peptides, proteins, hydraulic signals, and metabolites are transported mostly through the vasculature to distant tissues. How shoots and roots synchronize their response to stress using mobile signals is an emerging field of research. We summarize recent advances on mobile signals regulating shoot stomatal movement and root development in response to highly localized environmental cues. In addition, we highlight how the vascular system is not only a conduit but is also flexible in its development in response to abiotic stress., Competing Interests: Declaration of interests The authors declare no conflicts of interest., (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
47. Phenotyping Tomato Root Developmental Plasticity in Response to Salinity in Soil Rhizotrons.
- Author
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Gandullo J, Ahmad S, Darwish E, Karlova R, and Testerink C
- Abstract
Plants have developed multiple strategies to respond to salt stress. In order to identify new traits related to salt tolerance, with potential breeding application, the research focus has recently been shifted to include root system architecture (RSA) and root plasticity. Using a simple but effective root phenotyping system containing soil (rhizotrons), RSA of several tomato cultivars and their response to salinity was investigated. We observed a high level of root plasticity of tomato seedlings under salt stress. The general root architecture was substantially modified in response to salt, especially with respect to position of the lateral roots in the soil. At the soil surface, where salt accumulates, lateral root emergence was most strongly inhibited. Within the set of tomato cultivars, H1015 was the most tolerant to salinity in both developmental stages studied. A significant correlation between several root traits and aboveground growth parameters was observed, highlighting a possible role for regulation of both ion content and root architecture in salt stress resilience., Competing Interests: The authors declare that they have no conflicts of interests., (Copyright © 2021 Jacinto Gandullo et al.)
- Published
- 2021
- Full Text
- View/download PDF
48. Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress.
- Author
-
van der Meer T, Verlee A, Willems P, Impens F, Gevaert K, Testerink C, Stevens CV, Van Breusegem F, and Kerchev P
- Subjects
- Arabidopsis cytology, Arabidopsis drug effects, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Cell Death drug effects, Cell Respiration drug effects, Cell Respiration genetics, Computational Biology methods, Cyclopentanes metabolism, Gene Expression Profiling, Hydrogen Peroxide antagonists & inhibitors, Hydrogen Peroxide pharmacology, Hydroponics methods, Meristem cytology, Meristem drug effects, Meristem metabolism, Multienzyme Complexes metabolism, Oxylipins metabolism, Photosynthesis drug effects, Photosynthesis genetics, Plant Cells drug effects, Plant Cells metabolism, Plant Leaves cytology, Plant Leaves drug effects, Plant Leaves metabolism, Salicylic Acid metabolism, Seeds drug effects, Signal Transduction, Stress, Physiological, Sulfonamides chemical synthesis, Transcriptome, Arabidopsis genetics, Arabidopsis Proteins genetics, Gene Expression Regulation, Plant, Multienzyme Complexes genetics, Sulfonamides pharmacology
- Abstract
Alterations of hydrogen peroxide (H
2 O2 ) levels have a profound impact on numerous signaling cascades orchestrating plant growth, development, and stress signaling, including programmed cell death. To expand the repertoire of known molecular mechanisms implicated in H2 O2 signaling, we performed a forward chemical screen to identify small molecules that could alleviate the photorespiratory-induced cell death phenotype of Arabidopsis thaliana mutants lacking H2 O2 -scavenging capacity by peroxisomal catalase2. Here, we report the characterization of pakerine, an m -sulfamoyl benzamide from the sulfonamide family. Pakerine alleviates the cell death phenotype of cat2 mutants exposed to photorespiration-promoting conditions and delays dark-induced senescence in wild-type Arabidopsis leaves. By using a combination of transcriptomics, metabolomics, and affinity purification, we identified abnormal inflorescence meristem 1 (AIM1) as a putative protein target of pakerine. AIM1 is a 3-hydroxyacyl-CoA dehydrogenase involved in fatty acid β-oxidation that contributes to jasmonic acid (JA) and salicylic acid (SA) biosynthesis. Whereas intact JA biosynthesis was not required for pakerine bioactivity, our results point toward a role for β-oxidation-dependent SA production in the execution of H2 O2 -mediated cell death.- Published
- 2020
- Full Text
- View/download PDF
49. Salt Tolerance Mechanisms of Plants.
- Author
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van Zelm E, Zhang Y, and Testerink C
- Subjects
- Agriculture, Salt-Tolerant Plants genetics, Soil, Salinity, Salt Tolerance
- Abstract
Crop loss due to soil salinization is an increasing threat to agriculture worldwide. This review provides an overview of cellular and physiological mechanisms in plant responses to salt. We place cellular responses in a time- and tissue-dependent context in order to link them to observed phases in growth rate that occur in response to stress. Recent advances in phenotyping can now functionally or genetically link cellular signaling responses, ion transport, water management, and gene expression to growth, development, and survival. Halophytes, which are naturally salt-tolerant plants, are highlighted as success stories to learn from. We emphasize that ( a ) filling the major knowledge gaps in salt-induced signaling pathways, ( b ) increasing the spatial and temporal resolution of our knowledge of salt stress responses, ( c ) discovering and considering crop-specific responses, and ( d ) including halophytes in our comparative studies are all essential in order to take our approaches to increasing crop yields in saline soils to the next level.
- Published
- 2020
- Full Text
- View/download PDF
50. How Plants Sense and Respond to Stressful Environments.
- Author
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Lamers J, van der Meer T, and Testerink C
- Subjects
- Calcium metabolism, Reactive Oxygen Species metabolism, Signal Transduction, Environment, Plants metabolism, Stress, Physiological
- Abstract
Plants are exposed to an ever-changing environment to which they have to adjust accordingly. Their response is tightly regulated by complex signaling pathways that all start with stimulus perception. Here, we give an overview of the latest developments in the perception of various abiotic stresses, including drought, salinity, flooding, and temperature stress. We discuss whether proposed perception mechanisms are true sensors, which is well established for some abiotic factors but not yet fully elucidated for others. In addition, we review the downstream cellular responses, many of which are shared by various stresses but result in stress-specific physiological and developmental output. New sensing mechanisms have been identified, including heat sensing by the photoreceptor phytochrome B, salt sensing by glycosylinositol phosphorylceramide sphingolipids, and drought sensing by the specific calcium influx channel OSCA1. The simultaneous occurrence of multiple stress conditions shows characteristic downstream signaling signatures that were previously considered general signaling responses. The integration of sensing of multiple stress conditions and subsequent signaling responses is a promising venue for future research to improve the understanding of plant abiotic stress perception., (© 2020 American Society of Plant Biologists. All Rights Reserved.)
- Published
- 2020
- Full Text
- View/download PDF
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