143 results on '"Terol J"'
Search Results
2. A Multiagent System Proposal for 30 Day Readmission Problem Management
- Author
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Mateo Pla, M. A., primary, Zúñiga, L. Lemus, additional, Montañana, J. M., additional, Terol, J. Pons, additional, and Tortajada, S., additional
- Published
- 2015
- Full Text
- View/download PDF
3. IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers
- Author
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Ventimilla D, Velazquez K, Ruiz-Ruiz S, Terol J, Perez-Amador M, Vives M, Guerri J, Talon M, and Tadeo F
- Subjects
Gene silencing and overexpression ,Hormone peptide ,Cell separation ,fungi ,Abscission zone ,Abscission signaling module ,food and beverages ,LRR-RLKs ,Solanaceae - Abstract
Background Abscission is an active, organized, and highly coordinated cell separation process enabling the detachment of aerial organs through the modification of cell-to-cell adhesion and breakdown of cell walls at specific sites on the plant body known as abscission zones. In Arabidopsis thaliana, abscission of floral organs and cauline leaves is regulated by the interaction of the hormonal peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), a pair of redundant receptor-like protein kinases, HAESA (HAE) and HAESA-LIKE2 (HSL2), and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptors. However, the functionality of this abscission signaling module has not yet been demonstrated in other plant species. Results The expression of the pair of NbenIDA1 homeologs and the receptor NbenHAE.1 was supressed at the base of the corolla tube by the inoculation of two virus-induced gene silencing (VIGS) constructs in Nicotiana benthamiana. These gene suppression events arrested corolla abscission but did not produce any obvious effect on plant growth. VIGS plants retained a higher number of corollas attached to the flowers than control plants, an observation related to a greater corolla breakstrength. The arrest of corolla abscission was associated with the preservation of the parenchyma tissue at the base of the corolla tube that, in contrast, was virtually collapsed in normal corollas. In contrast, the inoculation of a viral vector construct that increased the expression of NbenIDA1A at the base of the corolla tube negatively affected the growth of the inoculated plants accelerating the timing of both corolla senescence and abscission. However, the heterologous ectopic overexpression of citrus CitIDA3 and Arabidopsis AtIDA in N. benthamiana did not alter the standard plant phenotype suggesting that the proteolytic processing machinery was unable to yield active peptides. Conclusion Here, we demonstrate that the pair of NbenIDA1 homeologs encoding small peptides of the IDA-like family and the receptor NbenHAE.1 control cellular breakdown at the base of the corolla tube awhere an adventitious AZ should be formed and, therefore, corolla abscission in N. benthamiana flowers. Altogether, our results provide the first evidence supporting the notion that the IDA-HAE/HSL2 signaling module is conserved in angiosperms.
- Published
- 2021
4. IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers
- Author
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Ventimilla, D, Velázquez, K, Ruiz-Ruiz, Susana, Terol, J, Pérez-Amador, Miguel A., Vives, M.ª.C, Guerri, José, Talon, M, Tadeo, F.R, Ventimilla, D, Velázquez, K, Ruiz-Ruiz, Susana, Terol, J, Pérez-Amador, Miguel A., Vives, M.ª.C, Guerri, José, Talon, M, and Tadeo, F.R
- Abstract
Background: Abscission is an active, organized, and highly coordinated cell separation process enabling the detachment of aerial organs through the modification of cell-to-cell adhesion and breakdown of cell walls at specific sites on the plant body known as abscission zones. In Arabidopsis thaliana, abscission of floral organs and cauline leaves is regulated by the interaction of the hormonal peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), a pair of redundant receptor-like protein kinases, HAESA (HAE) and HAESA-LIKE2 (HSL2), and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptors. However, the functionality of this abscission signaling module has not yet been demonstrated in other plant species. Results: The expression of the pair of NbenIDA1 homeologs and the receptor NbenHAE.1 was supressed at the base of the corolla tube by the inoculation of two virus-induced gene silencing (VIGS) constructs in Nicotiana benthamiana. These gene suppression events arrested corolla abscission but did not produce any obvious effect on plant growth. VIGS plants retained a higher number of corollas attached to the flowers than control plants, an observation related to a greater corolla breakstrength. The arrest of corolla abscission was associated with the preservation of the parenchyma tissue at the base of the corolla tube that, in contrast, was virtually collapsed in normal corollas. In contrast, the inoculation of a viral vector construct that increased the expression of NbenIDA1A at the base of the corolla tube negatively affected the growth of the inoculated plants accelerating the timing of both corolla senescence and abscission. However, the heterologous ectopic overexpression of citrus CitIDA3 and Arabidopsis AtIDA in N. benthamiana did not alter the standard plant phenotype suggesting that the proteolytic processing machinery was unable to yield active peptides. Conclusion: Here, we demonstrate that the pair of NbenIDA1 homeologs encoding small peptides of
- Published
- 2021
5. Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies
- Author
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Forment, J., Gadea, J., Huerta, L., Abizanda, L., Agusti, J., Alamar, S., Alos, E., Andres, F., Arribas, R., Beltran, J. P., Berbel, A., Blazquez, M. A., Brumos, J., Canas, L. A., Cercos, M., Colmenero-Flores, J. M., Conesa, A., Estables, B., Gandia, M., Garcia-Martinez, J. L., Gimeno, J., Gisbert, A., Gomez, G., Gonzalez-Candelas, L., Granell, A., Guerri, J., Lafuente, M. T., Madueno, F., Marcos, J. F., Marques, M. C., Martinez, F., Martinez-Godoy, M. A., Miralles, S., Moreno, P., Navarro, L., Pallas, V., Perez-Amador, M. A., Perez-Valle, J., Pons, C., Rodrigo, I., Rodriguez, P. L., Royo, C., Serrano, R., Soler, G., Tadeo, F., Talon, M., Terol, J., Trenor, M., Vaello, L., Vicente, O., Vidal, Ch., Zacarias, L., and Conejero, V.
- Published
- 2005
- Full Text
- View/download PDF
6. Nursing records with standardized nursing language in prehospital emergency care: from utopia to reality
- Author
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Morales, Juan Miguel, Rodriguez, M.M., Terol, J., Torres, A., and Alvarez, J.M.
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Nursing -- Research ,Health - Published
- 2003
7. Progress in Arabidopsis genome sequencing and functional genomics
- Author
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Wambutt, R, Murphy, G, Volckaert, G, Pohl, T, Düsterhöft, A, Stiekema, W, Entian, K.-D, Terryn, N, Harris, B, Ansorge, W, Brandt, P, Grivell, L, Rieger, M, Weichselgartner, M, de Simone, V, Obermaier, B, Mache, R, Müller, M, Kreis, M, Delseny, M, Puigdomenech, P, Watson, M, Schmidtheini, T, Reichert, B, Portatelle, D, Perez-Alonso, M, Boutry, M, Bancroft, I, Vos, P, Hoheisel, J, Zimmermann, W, Wedler, H, Ridley, P, Langham, S.-A, McCullagh, B, Bilham, L, Robben, J, Van der Schueren, J, Grymonprez, B, Chuang, Y.-J, Vandenbussche, F, Braeken, M, Weltjens, I, Voet, M, Bastiaens, I, Aert, R, Defoor, E, Weitzenegger, T, Bothe, G, Ramsperger, U, Hilbert, H, Braun, M, Holzer, E, Brandt, A, Peters, S, van Staveren, M, Dirkse, W, Mooijman, P, Klein Lankhorst, R, Rose, M, Hauf, J, Kötter, P, Berneiser, S, Hempel, S, Feldpausch, M, Lamberth, S, Van den Daele, H, De Keyser, A, Buysshaert, C, Gielen, J, Villarroel, R, De Clercq, R, Van Montagu, M, Rogers, J, Cronin, A, Quail, M, Bray-Allen, S, Clark, L, Doggett, J, Hall, S, Kay, M, Lennard, N, McLay, K, Mayes, R, Pettett, A, Rajandream, M.-A, Lyne, M, Benes, V, Rechmann, S, Borkova, D, Blöcker, H, Scharfe, M, Grimm, M, Löhnert, T.-H, Dose, S, de Haan, M, Maarse, A, Schäfer, M, Müller-Auer, S, Gabel, C, Fuchs, M, Fartmann, B, Granderath, K, Dauner, D, Herzl, A, Neumann, S, Argiriou, A, Vitale, D, Liguori, R, Piravandi, E, Massenet, O, Quigley, F, Clabauld, G, Mündlein, A, Felber, R, Schnabl, S, Hiller, R, Schmidt, W, Lecharny, A, Aubourg, S, Gy, I, Cooke, R, Berger, C, Monfort, A, Casacuberta, E, Gibbons, T, Weber, N, Vandenbol, M, Bargues, M, Terol, J, Torres, A, Perez-Perez, A, Purnelle, B, Bent, E, Johnson, S, Tacon, D, Jesse, T, Heijnen, L, Schwarz, S, Scholler, P, Heber, S, Bielke, C, Frishmann, D, Haase, D, Lemcke, K, Mewes, H.W, Stocker, S, Zaccaria, P, Mayer, K, Schüller, C, and Bevan, M
- Published
- 2000
- Full Text
- View/download PDF
8. [Renal artery stenosis in the transplant patient]
- Author
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Montañés Medina P, Rafael A. Medina-Lopez, Fj, Torrubia Romero, Domínguez Anguiano M, Cruz Navarro N, Rus Herrera F, Muñoz Terol J, and Jl, Rocha Castilla
- Subjects
Adult ,Reoperation ,Hypertension, Renovascular ,Postoperative Complications ,Humans ,Drug Therapy, Combination ,Treatment Failure ,Renal Artery Obstruction ,Combined Modality Therapy ,Kidney Transplantation ,Angioplasty, Balloon - Abstract
To present our experience in the diagnosis and treatment of renal artery stenosis in kidney transplants.A review of 601 renal transplants performed in adults showed 32 cases of renal artery stenosis. The diagnostic techniques utilized were arteriography in 18 patients, DIVAS in 15, echo-Doppler in 11 patients and MAG with captopril test on two occasions.Arterial hypertension was the most common symptom (92.8%), alone (53.1%) or in association with impaired renal function (43.7%). 46.8% of the cases could be managed by drug therapy. Percutaneous transluminal angioplasty was performed in 14 patients. Surgery was required on two occasions.The incidence of renal artery stenosis in our series of renal transplants in adults up to 1997 was 5.3%. Arterial hypertension with or without impairment of renal function was the most common symptom. Currently, echo-doppler and MAG with captopril test are the most widely utilized diagnostic techniques. Percutaneous transluminal angioplasty is the treatment of choice in renal artery stenosis when arterial hypertension is refractory to drug therapy. Good results are achieved in 57%, although it is not free from complications. In case of failure, revascularization surgery is the alternative approach.
- Published
- 1999
9. The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression Is regulated by low temperature but not by abscisic acid or dehydration
- Author
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Medina, J., Bargues, M., Terol, J., Pérez-Alonso, M., Julio Salinas, Terol, Javier, Salinas, Julio, Medina, Joaquin, Terol, Javier [0000-0003-3345-0078], Salinas, Julio [0000-0003-2020-0950], and Medina, Joaquin [0000-0002-1735-330X]
- Abstract
7 p.-5 fig., We have identified two genes from Arabidopsis that show high similarity with CBF1, a gene encoding an AP2 domain-containing transcriptional activator that binds to the low-temperature-responsive element CCGAC and induces the expression of some cold-regulated genes, increasing plant freezing tolerance. These two genes, which we have named CBF2 and CBF3, also encode proteins containing AP2 DNA-binding motifs.Furthermore, like CBF1, CBF2 and CBF3 proteins also include putative nuclear-localization signals and potential acidic activation domains. The CBF2 and CBF3 genes are linked to CBF1, constituting a cluster on the bottom arm of chromosome IV. The high level of similarity among the three CBF genes, their tandem organization, and the fact that they have the same transcriptional orientation all suggest a common origin. CBF1, CBF2, and CBF3 show identical expression patterns, being induced very rapidly by low-temperature treatment. However, in contrast to most of the cold-induced plant genes characterized, they are not responsive to abscisic acid or dehydration. Taken together, all of these data suggest that CBF2 and CBF3 may function as transcriptional activators, controlling the level of low-temperature gene expression and promoting freezing tolerance through an abscisic acid-independent pathway.
- Published
- 1999
10. GENE EXPRESSION DURING BUD DORMANCY RELEASE IN PRUNUS PERSICA
- Author
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Leida, C., primary, Terol, J., additional, Llácer, G., additional, Badenes, M., additional, Ríos, G., additional, Martí, G., additional, and Agustí, M., additional
- Published
- 2012
- Full Text
- View/download PDF
11. FUNCTIONAL GENOMIC APPROACHES TO UNDERSTANDING ABSCISSION ACTIVATION IN CITRUS
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Tadeo, F.R., primary, Agustí, J., additional, Merelo, P., additional, Cercós, M., additional, Terol, J., additional, Domingo, C., additional, and Talón, M., additional
- Published
- 2012
- Full Text
- View/download PDF
12. CITRUS GENOMICS AND BREEDING: IDENTIFICATION OF CANDIDATE GENES BY THE USE OF MUTANTS AND MICROARRAYS
- Author
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Talon, M., primary, Cercos, M., additional, Iglesias, D.J., additional, Colmenero-Flores, J.M., additional, Ibáñez, V., additional, Brumos, J., additional, Herrero-Ortega, M.A., additional, Rios, G., additional, Terol, J., additional, and Tadeo, F.R., additional
- Published
- 2011
- Full Text
- View/download PDF
13. Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization
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Leida, C., primary, Terol, J., additional, Marti, G., additional, Agusti, M., additional, Llacer, G., additional, Badenes, M. L., additional, and Rios, G., additional
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- 2010
- Full Text
- View/download PDF
14. High-throughput functional annotation and data mining with the Blast2GO suite
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Gotz, S., primary, Garcia-Gomez, J. M., additional, Terol, J., additional, Williams, T. D., additional, Nagaraj, S. H., additional, Nueda, M. J., additional, Robles, M., additional, Talon, M., additional, Dopazo, J., additional, and Conesa, A., additional
- Published
- 2008
- Full Text
- View/download PDF
15. In situ localization of the Antennapedia gene on the chromosomes of nine Drosophila species of the obscura group
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TEROL, J., primary, ALONSO, M. PEREZ, additional, and FRUTOS, R. DE, additional
- Published
- 2008
- Full Text
- View/download PDF
16. Progress in Arabidopsis genome sequencing and functional genomics
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UCL - SST/ISV - Institut des sciences de la vie, Wambutt, R, Murphy, G, Volckaert, G, Pohl, T, Düsterhöft, A, Stiekema, W, Entian, K.-D, Terryn, N, Harris, B, Ansorge, W, Brandt, P, Grivell, L, Rieger, M, Weichselgartner, M, de Simone, V, Obermaier, B, Mache, R, Müller, M, Kreis, M, Delseny, M, Puigdomenech, P, Watson, M, Schmidtheini, T, Reichert, B, Portatelle, D, Perez-Alonso, M, Boutry, Marc, Bancroft, I, Vos, P, Hoheisel, J, Zimmermann, W, Wedler, H, Ridley, P, Langham, S.-A, McCullagh, B, Bilham, L, Robben, J, Van der Schueren, J, Grymonprez, B, Chuang, Y.-J, Vandenbussche, F, Braeken, M, Weltjens, I, Voet, M, Bastiaens, I, Aert, R, Defoor, E, Weitzenegger, T, Bothe, G, Ramsperger, U, Hilbert, H, Braun, M, Holzer, E, Brandt, A, Peters, S, van Staveren, M, Dirkse, W, Mooijman, P, Klein Lankhorst, R, Rose, M, Hauf, J, Kötter, P, Berneiser, S, Hempel, S, Feldpausch, M, Lamberth, S, Van den Daele, H, De Keyser, A, Buysshaert, C, Gielen, J, Villarroel, R, De Clercq, R, Van Montagu, M, Rogers, J, Cronin, A, Quail, M, Bray-Allen, S, Clark, L, Doggett, J, Hall, S, Kay, M, Lennard, N, McLay, K, Mayes, R, Pettett, A, Rajandream, M.-A, Lyne, M, Benes, V, Rechmann, S, Borkova, D, Blöcker, H, Scharfe, M, Grimm, M, Löhnert, T.-H, Dose, S, de Haan, M, Maarse, A, Schäfer, M, Müller-Auer, S, Gabel, C, Fuchs, M, Fartmann, B, Granderath, K, Dauner, D, Herzl, A, Neumann, S, Argiriou, A, Vitale, D, Liguori, R, Piravandi, E, Massenet, O, Quigley, F, Clabauld, G, Mündlein, A, Felber, R, Schnabl, S, Hiller, R, Schmidt, W, Lecharny, A, Aubourg, S, Gy, I, Cooke, R, Berger, C, Monfort, A, Casacuberta, E, Gibbons, T, Weber, N, Vandenbol, M, Bargues, M, Terol, J, Torres, A, Perez-Perez, A, Purnelle, B, Bent, E, Johnson, S, Tacon, D, Jesse, T, Heijnen, L, Schwarz, S, Scholler, P, Heber, S, Bielke, C, Frishmann, D, Haase, D, Lemcke, K, Mewes, H.W, Stocker, S, Zaccaria, P, Mayer, K, Schüller, C, Bevan, M, UCL - SST/ISV - Institut des sciences de la vie, Wambutt, R, Murphy, G, Volckaert, G, Pohl, T, Düsterhöft, A, Stiekema, W, Entian, K.-D, Terryn, N, Harris, B, Ansorge, W, Brandt, P, Grivell, L, Rieger, M, Weichselgartner, M, de Simone, V, Obermaier, B, Mache, R, Müller, M, Kreis, M, Delseny, M, Puigdomenech, P, Watson, M, Schmidtheini, T, Reichert, B, Portatelle, D, Perez-Alonso, M, Boutry, Marc, Bancroft, I, Vos, P, Hoheisel, J, Zimmermann, W, Wedler, H, Ridley, P, Langham, S.-A, McCullagh, B, Bilham, L, Robben, J, Van der Schueren, J, Grymonprez, B, Chuang, Y.-J, Vandenbussche, F, Braeken, M, Weltjens, I, Voet, M, Bastiaens, I, Aert, R, Defoor, E, Weitzenegger, T, Bothe, G, Ramsperger, U, Hilbert, H, Braun, M, Holzer, E, Brandt, A, Peters, S, van Staveren, M, Dirkse, W, Mooijman, P, Klein Lankhorst, R, Rose, M, Hauf, J, Kötter, P, Berneiser, S, Hempel, S, Feldpausch, M, Lamberth, S, Van den Daele, H, De Keyser, A, Buysshaert, C, Gielen, J, Villarroel, R, De Clercq, R, Van Montagu, M, Rogers, J, Cronin, A, Quail, M, Bray-Allen, S, Clark, L, Doggett, J, Hall, S, Kay, M, Lennard, N, McLay, K, Mayes, R, Pettett, A, Rajandream, M.-A, Lyne, M, Benes, V, Rechmann, S, Borkova, D, Blöcker, H, Scharfe, M, Grimm, M, Löhnert, T.-H, Dose, S, de Haan, M, Maarse, A, Schäfer, M, Müller-Auer, S, Gabel, C, Fuchs, M, Fartmann, B, Granderath, K, Dauner, D, Herzl, A, Neumann, S, Argiriou, A, Vitale, D, Liguori, R, Piravandi, E, Massenet, O, Quigley, F, Clabauld, G, Mündlein, A, Felber, R, Schnabl, S, Hiller, R, Schmidt, W, Lecharny, A, Aubourg, S, Gy, I, Cooke, R, Berger, C, Monfort, A, Casacuberta, E, Gibbons, T, Weber, N, Vandenbol, M, Bargues, M, Terol, J, Torres, A, Perez-Perez, A, Purnelle, B, Bent, E, Johnson, S, Tacon, D, Jesse, T, Heijnen, L, Schwarz, S, Scholler, P, Heber, S, Bielke, C, Frishmann, D, Haase, D, Lemcke, K, Mewes, H.W, Stocker, S, Zaccaria, P, Mayer, K, Schüller, C, and Bevan, M
- Published
- 2000
17. Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana
- Author
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UCL - SST/ISV - Institut des sciences de la vie, Mayer, K., Schüller, C., Wambutt, R., Murphy, G., Volckaert, G., Pohl, T., Düsterhöft, A., Stiekema, W., Entian, K.-D., Terryn, N., Harris, B., Ansorge, W., Brandt, P., Grivell, L., Rieger, M., Weichselgartner, M., de Simone, V., Obermaier, B., Mache, R., Müller, M., Kreis, M., Delseny, M., Puigdomenech, P., Watson, M., Schmidtheini, T., Reichert, B., Portatelle, D., Perez-Alonso, M., Boutry, Marc, Bancroft, I., Vos, P., Hoheisel, J., Zimmermann, W., Wedler, H., Ridley, P., Langham, S.-A., McCullagh, B., Bilham, L., Robben, J., Van der Schueren, J., Grymonprez, B., Chuang, Y.-J., Vandenbussche, F., Braeken, M., Weltjens, I., Voet, M., Bastiaens, I., Aert, R., Defoor, E., Weitzenegger, T., Bothe, G., Ramsperger, U., Hilbert, H., Braun, M., Holzer, E., Brandt, A., Peters, S., van Staveren, M., Dirkse, W., Mooijman, P., Lankhorst, R. Klein, Rose, M., Hauf, J., Kötter, P., Berneiser, S., Hempel, S., Feldpausch, M., Lamberth, S., Van den Daele, H., De Keyser, A., Buysshaert, C., Gielen, J., Villarroel, R., De Clercq, R., Van Montagu, M., Rogers, J., Cronin, A., Quail, M., Bray-Allen, S., Clark, L., Doggett, J., Hall, S., Kay, M., Lennard, N., McLay, K., Mayes, R., Pettett, A., Rajandream, M.-A., Lyne, M., Benes, V., Rechmann, S., Borkova, D., Blöcker, H., Scharfe, M., Grimm, M., Löhnert, T.-H., Dose, S., de Haan, M., Maarse, A., Schäfer, M., Müller-Auer, S., Gabel, C., Fuchs, M., Fartmann, B., Granderath, K., Dauner, D., Herzl, A., Neumann, S., Argiriou, A., Vitale, D., Liguori, R., Piravandi, E., Massenet, O., Quigley, F., Clabauld, G., Mündlein, A., Felber, R., Schnabl, S., Hiller, R., Schmidt, W., Lecharny, A., Aubourg, S., Chefdor, F., Cooke, R., Berger, C., Montfort, A., Casacuberta, E., Gibbons, T., Weber, N., Vandenbol, M., Bargues, M., Terol, J., Torres, A., Perez-Perez, A., Purnelle, B., Bent, E., Johnson, S., Tacon, D., Jesse, T., Heijnen, L., Schwarz, S., Scholler, P., Heber, S., Francs, P., Bielke, C., Frishman, D., Haase, D., Lemcke, K., Mewes, H. W., Stocker, S., Zaccaria, P., Wilson, R. K., de la Bastide, M., Habermann, K., Parnell, L., Dedhia, N., Gnoj, L., Schutz, K., Huang, E., Spiegel, L., Sehkon, M., Murray, J., Sheet, P., Cordes, M., Abu-Threideh, J., Stoneking, T., Kalicki, J., Graves, T., Harmon, G., Edwards, J., Latreille, P., Courtney, L., Cloud, J., Abbott, A., Scott, K., Johnson, D., Minx, P., Bentley, D., Fulton, B., Miller, N., Greco, T., Kemp, K., Kramer, J., Fulton, L., Mardis, E., Dante, M., Pepin, K., Hillier, L., Nelson, J., Spieth, J., Ryan, E., Andrews, S., Geisel, C., Layman, D., Du, H., Ali, J., Berghoff, A., Jones, K., Drone, K., Cotton, M., Joshu, C., Antonoiu, B., Zidanic, M., Strong, C., Sun, H., Lamar, B., Yordan, C., Ma, P., Zhong, J., Preston, R., Vil, D., Shekher, M., Matero, A., Shah, R., Swaby, I'K., O'Shaughnessy, A., Rodriguez, M., Hoffman, J., Till, S., Granat, S., Shohdy, N., Hasegawa, A., Hameed, A., Lodhi, M., Johnson, A., Chen, E., Marra, M., Martienssen, R., McCombie, W. R., UCL - SST/ISV - Institut des sciences de la vie, Mayer, K., Schüller, C., Wambutt, R., Murphy, G., Volckaert, G., Pohl, T., Düsterhöft, A., Stiekema, W., Entian, K.-D., Terryn, N., Harris, B., Ansorge, W., Brandt, P., Grivell, L., Rieger, M., Weichselgartner, M., de Simone, V., Obermaier, B., Mache, R., Müller, M., Kreis, M., Delseny, M., Puigdomenech, P., Watson, M., Schmidtheini, T., Reichert, B., Portatelle, D., Perez-Alonso, M., Boutry, Marc, Bancroft, I., Vos, P., Hoheisel, J., Zimmermann, W., Wedler, H., Ridley, P., Langham, S.-A., McCullagh, B., Bilham, L., Robben, J., Van der Schueren, J., Grymonprez, B., Chuang, Y.-J., Vandenbussche, F., Braeken, M., Weltjens, I., Voet, M., Bastiaens, I., Aert, R., Defoor, E., Weitzenegger, T., Bothe, G., Ramsperger, U., Hilbert, H., Braun, M., Holzer, E., Brandt, A., Peters, S., van Staveren, M., Dirkse, W., Mooijman, P., Lankhorst, R. Klein, Rose, M., Hauf, J., Kötter, P., Berneiser, S., Hempel, S., Feldpausch, M., Lamberth, S., Van den Daele, H., De Keyser, A., Buysshaert, C., Gielen, J., Villarroel, R., De Clercq, R., Van Montagu, M., Rogers, J., Cronin, A., Quail, M., Bray-Allen, S., Clark, L., Doggett, J., Hall, S., Kay, M., Lennard, N., McLay, K., Mayes, R., Pettett, A., Rajandream, M.-A., Lyne, M., Benes, V., Rechmann, S., Borkova, D., Blöcker, H., Scharfe, M., Grimm, M., Löhnert, T.-H., Dose, S., de Haan, M., Maarse, A., Schäfer, M., Müller-Auer, S., Gabel, C., Fuchs, M., Fartmann, B., Granderath, K., Dauner, D., Herzl, A., Neumann, S., Argiriou, A., Vitale, D., Liguori, R., Piravandi, E., Massenet, O., Quigley, F., Clabauld, G., Mündlein, A., Felber, R., Schnabl, S., Hiller, R., Schmidt, W., Lecharny, A., Aubourg, S., Chefdor, F., Cooke, R., Berger, C., Montfort, A., Casacuberta, E., Gibbons, T., Weber, N., Vandenbol, M., Bargues, M., Terol, J., Torres, A., Perez-Perez, A., Purnelle, B., Bent, E., Johnson, S., Tacon, D., Jesse, T., Heijnen, L., Schwarz, S., Scholler, P., Heber, S., Francs, P., Bielke, C., Frishman, D., Haase, D., Lemcke, K., Mewes, H. W., Stocker, S., Zaccaria, P., Wilson, R. K., de la Bastide, M., Habermann, K., Parnell, L., Dedhia, N., Gnoj, L., Schutz, K., Huang, E., Spiegel, L., Sehkon, M., Murray, J., Sheet, P., Cordes, M., Abu-Threideh, J., Stoneking, T., Kalicki, J., Graves, T., Harmon, G., Edwards, J., Latreille, P., Courtney, L., Cloud, J., Abbott, A., Scott, K., Johnson, D., Minx, P., Bentley, D., Fulton, B., Miller, N., Greco, T., Kemp, K., Kramer, J., Fulton, L., Mardis, E., Dante, M., Pepin, K., Hillier, L., Nelson, J., Spieth, J., Ryan, E., Andrews, S., Geisel, C., Layman, D., Du, H., Ali, J., Berghoff, A., Jones, K., Drone, K., Cotton, M., Joshu, C., Antonoiu, B., Zidanic, M., Strong, C., Sun, H., Lamar, B., Yordan, C., Ma, P., Zhong, J., Preston, R., Vil, D., Shekher, M., Matero, A., Shah, R., Swaby, I'K., O'Shaughnessy, A., Rodriguez, M., Hoffman, J., Till, S., Granat, S., Shohdy, N., Hasegawa, A., Hameed, A., Lodhi, M., Johnson, A., Chen, E., Marra, M., Martienssen, R., and McCombie, W. R.
- Abstract
The higher plant Arabidopsis thaliana (Arabidopsis) is an important model for identifying plant genes and determining their function. To assist biological investigations and to define chromosome structure, a coordinated effort to sequence the Arabidopsis genome was initiated in late 1996. Here we report one of the first milestones of this project, the sequence of chromosome 4. Analysis of 17.38 megabases of unique sequence, representing about 17% of the genome, reveals 3,744 protein coding genes, 81 transfer RNAs and numerous repeat elements. Heterochromatic regions surrounding the putative centromere, which has not yet been completely sequenced, are characterized by an increased frequency of a variety of repeats, new repeats, reduced recombination, lowered gene density and lowered gene expression. Roughly 60% of the predicted protein-coding genes have been functionally characterized on the basis of their homology to known genes. Many genes encode predicted proteins that are homologous to human and Caenorhabditis elegans proteins.
- Published
- 1999
18. The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration
- Author
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Medina, Joaquín, Bargues, M., Terol, J., Pérez-Alonso, Manuel, Salinas, Julio, Medina, Joaquín, Bargues, M., Terol, J., Pérez-Alonso, Manuel, and Salinas, Julio
- Abstract
We have identified two genes from Arabidopsis that show high similarity with CBF1, a gene encoding an AP2 domain-containing transcriptional activator that binds to the low-temperature-responsive element CCGAC and induces the expression of some cold-regulated genes, increasing plant freezing tolerance. These two genes, which we have named CBF2 and CBF3, also encode proteins containing AP2 DNA-binding motifs. Furthermore, like CBF1, CBF2 and CBF3 proteins also include putative nuclear-localization signals and potential acidic activation domains. The CBF2 and CBF3 genes are linked to CBF1, constituting a cluster on the bottom arm of chromosome IV. The high level of similarity among the three CBF genes, their tandem organization, and the fact that they have the same transcriptional orientation all suggest a common origin. CBF1, CBF2, and CBF3 show identical expression patterns, being induced very rapidly by low-temperature treatment. However, in contrast to most of the cold-induced plant genes characterized, they are not responsive to abscisic acid or dehydration. Taken together, all of these data suggest that CBF2 and CBF3 may function as transcriptional activators, controlling the level of low-temperature gene expression and promoting freezing tolerance through an abscisic acid-independent pathway.
- Published
- 1999
19. Peripheral arterial disease in patients with stages IV and V chronic renal failure
- Author
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Guerrero, A., primary, Montes, R., additional, Munoz-Terol, J., additional, Gil-Peralta, A., additional, Toro, J., additional, Naranjo, M., additional, Gonzalez-Perez, P., additional, Martin-Herrera, C., additional, and Ruiz-Fernandez, A., additional
- Published
- 2006
- Full Text
- View/download PDF
20. Random Genome Sequencing of Ralstonia solanacearum Strain IVIA 1602 and Comparative Analysis with Strain GMI1000
- Author
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Terol, J., primary, Munoz-Soriano, V., additional, Arahal, D. R., additional, Buades, C., additional, Perez-Perez, A., additional, Llop, P., additional, Belmonte, U. C. F., additional, Lopez, M. M., additional, and Perez-Alonso, M., additional
- Published
- 2006
- Full Text
- View/download PDF
21. ZFWD: a novel subfamily of plant proteins containing a C3H zinc finger and seven WD40 repeats
- Author
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Terol, J, primary, Bargues, M, additional, and Pérez-Alonso, M, additional
- Published
- 2000
- Full Text
- View/download PDF
22. Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana
- Author
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Mayer, K., primary, Schüller, C., additional, Wambutt, R., additional, Murphy, G., additional, Volckaert, G., additional, Pohl, T., additional, Düsterhöft, A., additional, Stiekema, W., additional, Entian, K.-D., additional, Terryn, N., additional, Harris, B., additional, Ansorge, W., additional, Brandt, P., additional, Grivell, L., additional, Rieger, M., additional, Weichselgartner, M., additional, de Simone, V., additional, Obermaier, B., additional, Mache, R., additional, Müller, M., additional, Kreis, M., additional, Delseny, M., additional, Puigdomenech, P., additional, Watson, M., additional, Schmidtheini, T., additional, Reichert, B., additional, Portatelle, D., additional, Perez-Alonso, M., additional, Boutry, M., additional, Bancroft, I., additional, Vos, P., additional, Hoheisel, J., additional, Zimmermann, W., additional, Wedler, H., additional, Ridley, P., additional, Langham, S.-A., additional, McCullagh, B., additional, Bilham, L., additional, Robben, J., additional, Van der Schueren, J., additional, Grymonprez, B., additional, Chuang, Y.-J., additional, Vandenbussche, F., additional, Braeken, M., additional, Weltjens, I., additional, Voet, M., additional, Bastiaens, I., additional, Aert, R., additional, Defoor, E., additional, Weitzenegger, T., additional, Bothe, G., additional, Ramsperger, U., additional, Hilbert, H., additional, Braun, M., additional, Holzer, E., additional, Brandt, A., additional, Peters, S., additional, van Staveren, M., additional, Dirkse, W., additional, Mooijman, P., additional, Lankhorst, R. Klein, additional, Rose, M., additional, Hauf, J., additional, Kötter, P., additional, Berneiser, S., additional, Hempel, S., additional, Feldpausch, M., additional, Lamberth, S., additional, Van den Daele, H., additional, De Keyser, A., additional, Buysshaert, C., additional, Gielen, J., additional, Villarroel, R., additional, De Clercq, R., additional, Van Montagu, M., additional, Rogers, J., additional, Cronin, A., additional, Quail, M., additional, Bray-Allen, S., additional, Clark, L., additional, Doggett, J., additional, Hall, S., additional, Kay, M., additional, Lennard, N., additional, McLay, K., additional, Mayes, R., additional, Pettett, A., additional, Rajandream, M.-A., additional, Lyne, M., additional, Benes, V., additional, Rechmann, S., additional, Borkova, D., additional, Blöcker, H., additional, Scharfe, M., additional, Grimm, M., additional, Löhnert, T.-H., additional, Dose, S., additional, de Haan, M., additional, Maarse, A., additional, Schäfer, M., additional, Müller-Auer, S., additional, Gabel, C., additional, Fuchs, M., additional, Fartmann, B., additional, Granderath, K., additional, Dauner, D., additional, Herzl, A., additional, Neumann, S., additional, Argiriou, A., additional, Vitale, D., additional, Liguori, R., additional, Piravandi, E., additional, Massenet, O., additional, Quigley, F., additional, Clabauld, G., additional, Mündlein, A., additional, Felber, R., additional, Schnabl, S., additional, Hiller, R., additional, Schmidt, W., additional, Lecharny, A., additional, Aubourg, S., additional, Chefdor, F., additional, Cooke, R., additional, Berger, C., additional, Montfort, A., additional, Casacuberta, E., additional, Gibbons, T., additional, Weber, N., additional, Vandenbol, M., additional, Bargues, M., additional, Terol, J., additional, Torres, A., additional, Perez-Perez, A., additional, Purnelle, B., additional, Bent, E., additional, Johnson, S., additional, Tacon, D., additional, Jesse, T., additional, Heijnen, L., additional, Schwarz, S., additional, Scholler, P., additional, Heber, S., additional, Francs, P., additional, Bielke, C., additional, Frishman, D., additional, Haase, D., additional, Lemcke, K., additional, Mewes, H. W., additional, Stocker, S., additional, Zaccaria, P., additional, Bevan, M., additional, Wilson, R. K., additional, de la Bastide, M., additional, Habermann, K., additional, Parnell, L., additional, Dedhia, N., additional, Gnoj, L., additional, Schutz, K., additional, Huang, E., additional, Spiegel, L., additional, Sehkon, M., additional, Murray, J., additional, Sheet, P., additional, Cordes, M., additional, Abu-Threideh, J., additional, Stoneking, T., additional, Kalicki, J., additional, Graves, T., additional, Harmon, G., additional, Edwards, J., additional, Latreille, P., additional, Courtney, L., additional, Cloud, J., additional, Abbott, A., additional, Scott, K., additional, Johnson, D., additional, Minx, P., additional, Bentley, D., additional, Fulton, B., additional, Miller, N., additional, Greco, T., additional, Kemp, K., additional, Kramer, J., additional, Fulton, L., additional, Mardis, E., additional, Dante, M., additional, Pepin, K., additional, Hillier, L., additional, Nelson, J., additional, Spieth, J., additional, Ryan, E., additional, Andrews, S., additional, Geisel, C., additional, Layman, D., additional, Du, H., additional, Ali, J., additional, Berghoff, A., additional, Jones, K., additional, Drone, K., additional, Cotton, M., additional, Joshu, C., additional, Antonoiu, B., additional, Zidanic, M., additional, Strong, C., additional, Sun, H., additional, Lamar, B., additional, Yordan, C., additional, Ma, P., additional, Zhong, J., additional, Preston, R., additional, Vil, D., additional, Shekher, M., additional, Matero, A., additional, Shah, R., additional, Swaby, I'K., additional, O'Shaughnessy, A., additional, Rodriguez, M., additional, Hoffman, J., additional, Till, S., additional, Granat, S., additional, Shohdy, N., additional, Hasegawa, A., additional, Hameed, A., additional, Lodhi, M., additional, Johnson, A., additional, Chen, E., additional, Marra, M., additional, Martienssen, R., additional, and McCombie, W. R., additional
- Published
- 1999
- Full Text
- View/download PDF
23. Poly(dC) segments and cloning artifacts in databases.
- Author
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Paricio, N, primary, Terol, J, additional, Artero, R D, additional, and Pérez-Alonso, M, additional
- Published
- 1995
- Full Text
- View/download PDF
24. Continuous flow photocatalytic degradation of carbaryl in aqueous media
- Author
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Peris-Cardells, E., primary, Terol, J., additional, Mauri, A. R., additional, de la Guardia, M., additional, and Pramauro, E., additional
- Published
- 1993
- Full Text
- View/download PDF
25. Acetylation Phenotype and Hepatotoxicity in the Treatment of Tuberculosis in Children.
- Author
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Martinez-Roig, A., Cami, J., Llorens-Terol, J., de la Torre, R., and Perich, F.
- Published
- 1986
- Full Text
- View/download PDF
26. The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression Is regulated by low temperature but not by abscisic acid or dehydration.
- Author
-
Medina, J, Bargues, M, Terol, J, Pérez-Alonso, M, and Salinas, J
- Abstract
We have identified two genes from Arabidopsis that show high similarity with CBF1, a gene encoding an AP2 domain-containing transcriptional activator that binds to the low-temperature-responsive element CCGAC and induces the expression of some cold-regulated genes, increasing plant freezing tolerance. These two genes, which we have named CBF2 and CBF3, also encode proteins containing AP2 DNA-binding motifs. Furthermore, like CBF1, CBF2 and CBF3 proteins also include putative nuclear-localization signals and potential acidic activation domains. The CBF2 and CBF3 genes are linked to CBF1, constituting a cluster on the bottom arm of chromosome IV. The high level of similarity among the three CBF genes, their tandem organization, and the fact that they have the same transcriptional orientation all suggest a common origin. CBF1, CBF2, and CBF3 show identical expression patterns, being induced very rapidly by low-temperature treatment. However, in contrast to most of the cold-induced plant genes characterized, they are not responsive to abscisic acid or dehydration. Taken together, all of these data suggest that CBF2 and CBF3 may function as transcriptional activators, controlling the level of low-temperature gene expression and promoting freezing tolerance through an abscisic acid-independent pathway.
- Published
- 1999
- Full Text
- View/download PDF
27. Brucellosis treated with rifampicin.
- Author
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LLORENS-TEROL, J. and BUSQUETS, R. M.
- Abstract
14 children, aged between 15 months and 14 years, with brucellosis were treated with oral rifampicin only (20 mg/kg per day) for 3 weeks. Laboratory diagnosis depended on blood culture (positive for Brucella melitensis in 11 of the cases), serum agglutination, complement-fixation test, and Coomb's test. Response was good in each child, with fever clearing between the 2nd and 8th day. 2 children relapsed, but one relapse was probably a reinfection from contaminated cheese. Both children were given a further course of treatment (rifampicin and co-trimoxazole) which was successful. Despite the reasonably good results with rifampicin alone, it is advisable to combine the drug with co-trimoxazole when treating brucellosis. [ABSTRACT FROM AUTHOR]
- Published
- 1980
- Full Text
- View/download PDF
28. STROKE DUE TO FIBROMUSCULAR HYPERPLASIA OF THE INTERNAL CAROTID ARTERY.
- Author
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LLORENS-TEROL, J., SOLE-LLENAS, J., and TURA, A.
- Published
- 1983
- Full Text
- View/download PDF
29. Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana
- Author
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Salanoubat, M., Lemcke, K., Rieger, M., Ansorge, W., Unseld, M., Fartmann, B., Valle, G., Blocker, H., Perez-Alonso, M., Obermaier, B., Delseny, M., Boutry, M., Grivell, La, Mache, R., Puigdomenech, P., Simone, V., Choisne, N., Artiguenave, F., Robert, C., Brottier, P., Wincker, P., Cattolico, L., Weissenbach, J., Saurin, W., Quetier, F., Schafer, M., Muller-Auer, S., Gabel, C., Fuchs, M., Benes, V., Wurmbach, E., Drzonek, H., Erfle, H., Jordan, N., Bangert, S., Wiedelmann, R., Kranz, H., Voss, H., Holland, R., Brandt, P., Nyakatura, G., Vezzi, A., D Angelo, M., Alberto Pallavicini, Toppo, S., Simionati, B., Conrad, A., Hornischer, K., Kauer, G., Lohnert, Th, Nordsiek, G., Reichelt, J., Scharfe, M., Schon, O., Bargues, M., Terol, J., Climent, J., Navarro, P., Collado, C., Perez-Perez, A., Ottenwalder, B., Duchemin, D., Cooke, R., Laudie, M., Berger-Llauro, C., Purnelle, B., Masuy, D., Haan, M., Maarse, Ac, Alcaraz, Jp, Cottet, A., Casacuberta, E., Monfort, A., Argiriou, A., Flores, M., Liguori, R., Vitale, D., Mannhaupt, G., Haase, D., Schoof, H., Rudd, S., Zaccaria, P., Mewes, Hw, Mayer, Kfx, Kaul, S., Town, Cd, Koo, Hl, Tallon, Lj, Jenkins, J., Rooney, T., Rizzo, M., Walts, A., Utterback, T., Fujii, Cy, Shea, Tp, Creasy, Th, Haas, B., Maiti, R., Wu, Dy, Peterson, J., Aken, S., Pai, G., Militscher, J., Sellers, P., Gill, Je, Feldblyum, Tv, Preuss, D., Lin, Xy, Nierman, Wc, Salzberg, Sl, White, O., Venter, Jc, Fraser, Cm, Kaneko, T., Nakamura, Y., Sato, S., Kato, T., Asamizu, E., Sasamoto, S., Kimura, T., Idesawa, K., Kawashima, K., Kishida, Y., Kiyokawa, C., Kohara, M., Matsumoto, M., Matsuno, A., Muraki, A., Nakayama, S., Nakazaki, N., Shinpo, S., Takeuchi, C., Wada, T., Watanabe, A., Yamada, M., Yasuda, M., Tabata, S., European Union Chromosome 3 Arabid, Inst Genomic Res, and Dna, Kazusa Res Inst
30. Functional Genomic Approaches to Understanding Abscission Activation in Citrus
- Author
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Francisco Tadeo, Agusti, J., Merelo, P., Cercos, M., Terol, J., Domingo, C., Talon, M., Litz, Re, Folta, Km, and Alfaro, Fp
31. Citrus Genomics and Breeding: Identification of Candidate Genes by the Use of Mutants and Microarrays
- Author
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Talon, M., Cercos, M., Iglesias, D. J., Colmenero-Flores, J. M., Ibanez, V., Brumos, J., Herrero-Ortega, M. A., Rios, G., Terol, J., Tadeo, F. R., Gentile, A., and Lamalfa, S.
32. Citrus Genomics and Breeding: Identification of Candidate Genes by the Use of Mutants and Microarrays
- Author
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Talon, M., Cercos, M., Iglesias, D. J., Colmenero-Flores, J. M., Ibanez, V., Brumos, J., Herrero-Ortega, M. A., Rios, G., Terol, J., and Francisco Tadeo
33. Citrus genomics and breeding: Identification of candidate genes by the use of mutants and microarrays
- Author
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Talon, M., Cercos, M., Iglesias, D. J., Jose Manuel Colmenero-Flores, Ibáñez, V., Brumos, J., Herrero-Ortega, M. A., Rios, G., Terol, J., and Tadeo, F. R.
34. ALTERNATIVE THERAPY OF TYPHOID FEVER IN CHILDREN
- Author
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Llorens-Terol, J, primary, Martinez-Roig, A, additional, Mur, A, additional, and Tauler, E, additional
- Published
- 1985
- Full Text
- View/download PDF
35. TRIMETHOPRIM AND CO-TRIMOXAZOLE IN THE TREATMENT OF PNEUMONA IN CHILDREN”: A DOUBLE-BLIND CLINICAL TRIAL
- Author
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Mrtinez-Roig, A, primary, Llorens-Terol, J, additional, Tauler, E, additional, and Mur, A, additional
- Published
- 1985
- Full Text
- View/download PDF
36. ASTHMA AND ENVIRONMENTAL FACTORS
- Author
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Tauler, E, primary, Llorens-Terol, J, additional, Mur, A, additional, and Leal, C, additional
- Published
- 1985
- Full Text
- View/download PDF
37. A FOLLOW-UP STUDY OF 24 CHILDREN BORN TO INTRAVENOUS DRUG USERS INFECTED WITH HIV
- Author
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Marti-Gaudes, C, primary, Llorens-Terol, J, additional, and Mur, A, additional
- Published
- 1987
- Full Text
- View/download PDF
38. A reference genetic map of C. clementina hort. ex Tan.; citrus evolution inferences from comparative mapping
- Author
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Ollitrault Patrick, Terol Javier, Chen Chunxian, Federici Claire T, Lotfy Samia, Hippolyte Isabelle, Ollitrault Frédérique, Bérard Aurélie, Chauveau Aurélie, Cuenca Jose, Costantino Gilles, Kacar Yildiz, Mu Lisa, Garcia-Lor Andres, Froelicher Yann, Aleza Pablo, Boland Anne, Billot Claire, Navarro Luis, Luro François, Roose Mikeal L, Gmitter Frederick G, Talon Manuel, and Brunel Dominique
- Subjects
C. clementina ,C. sinensis ,C. maxima ,SSRs ,SNPs ,Indels ,Genetic maps ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Most modern citrus cultivars have an interspecific origin. As a foundational step towards deciphering the interspecific genome structures, a reference whole genome sequence was produced by the International Citrus Genome Consortium from a haploid derived from Clementine mandarin. The availability of a saturated genetic map of Clementine was identified as an essential prerequisite to assist the whole genome sequence assembly. Clementine is believed to be a ‘Mediterranean’ mandarin × sweet orange hybrid, and sweet orange likely arose from interspecific hybridizations between mandarin and pummelo gene pools. The primary goals of the present study were to establish a Clementine reference map using codominant markers, and to perform comparative mapping of pummelo, sweet orange, and Clementine. Results Five parental genetic maps were established from three segregating populations, which were genotyped with Single Nucleotide Polymorphism (SNP), Simple Sequence Repeats (SSR) and Insertion-Deletion (Indel) markers. An initial medium density reference map (961 markers for 1084.1 cM) of the Clementine was established by combining male and female Clementine segregation data. This Clementine map was compared with two pummelo maps and a sweet orange map. The linear order of markers was highly conserved in the different species. However, significant differences in map size were observed, which suggests a variation in the recombination rates. Skewed segregations were much higher in the male than female Clementine mapping data. The mapping data confirmed that Clementine arose from hybridization between ‘Mediterranean’ mandarin and sweet orange. The results identified nine recombination break points for the sweet orange gamete that contributed to the Clementine genome. Conclusions A reference genetic map of citrus, used to facilitate the chromosome assembly of the first citrus reference genome sequence, was established. The high conservation of marker order observed at the interspecific level should allow reasonable inferences of most citrus genome sequences by mapping next-generation sequencing (NGS) data in the reference genome sequence. The genome of the haploid Clementine used to establish the citrus reference genome sequence appears to have been inherited primarily from the ‘Mediterranean’ mandarin. The high frequency of skewed allelic segregations in the male Clementine data underline the probable extent of deviation from Mendelian segregation for characters controlled by heterozygous loci in male parents.
- Published
- 2012
- Full Text
- View/download PDF
39. SNP mining in C. clementina BAC end sequences; transferability in the Citrus genus (Rutaceae), phylogenetic inferences and perspectives for genetic mapping
- Author
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Ollitrault Patrick, Terol Javier, Garcia-Lor Andres, Bérard Aurélie, Chauveau Aurélie, Froelicher Yann, Belzile Caroline, Morillon Raphaël, Navarro Luis, Brunel Dominique, and Talon Manuel
- Subjects
Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background With the increasing availability of EST databases and whole genome sequences, SNPs have become the most abundant and powerful polymorphic markers. However, SNP chip data generally suffers from ascertainment biases caused by the SNP discovery and selection process in which a small number of individuals are used as discovery panels. The ongoing International Citrus Genome Consortium sequencing project of the highly heterozygous Clementine and sweet orange genomes will soon result in the release of several hundred thousand SNPs. The primary goals of this study were: (i) to estimate the transferability within the genus Citrus of SNPs discovered from Clementine BACend sequencing (BES), (ii) to estimate bias associated with the very narrow discovery panel, and (iii) to evaluate the usefulness of the Clementine-derived SNP markers for diversity analysis and comparative mapping studies between the different cultivated Citrus species. Results Fifty-four accessions covering the main Citrus species and 52 interspecific hybrids between pummelo and Clementine were genotyped on a GoldenGate array platform using 1,457 SNPs mined from Clementine BES and 37 SNPs identified between and within C. maxima, C. medica, C. reticulata and C. micrantha. Consistent results were obtained from 622 SNP loci. Of these markers, 116 displayed incomplete transferability primarily in C. medica, C. maxima and wild Citrus species. The two primary biases associated with the SNP mining in Clementine were an overestimation of the C. reticulata diversity and an underestimation of the interspecific differentiation. However, the genetic stratification of the gene pool was high, with very frequent significant linkage disequilibrium. Furthermore, the shared intraspecific polymorphism and accession heterozygosity were generally enough to perform interspecific comparative genetic mapping. Conclusions A set of 622 SNP markers providing consistent results was selected. Of the markers mined from Clementine, 80.5% were successfully transferred to the whole Citrus gene pool. Despite the ascertainment biases in relation to the Clementine origin, the SNP data confirm the important stratification of the gene pools around C. maxima, C. medica and C. reticulata as well as previous hypothesis on the origin of secondary species. The implemented SNP marker set will be very useful for comparative genetic mapping in Citrus and genetic association in C. reticulata.
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- 2012
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40. The aconitate hydratase family from Citrus
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Cercos Manuel, Talon Manuel, Soler Guillermo, and Terol Javier
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Botany ,QK1-989 - Abstract
Abstract Background Research on citrus fruit ripening has received considerable attention because of the importance of citrus fruits for the human diet. Organic acids are among the main determinants of taste and organoleptic quality of fruits and hence the control of fruit acidity loss has a strong economical relevance. In citrus, organic acids accumulate in the juice sac cells of developing fruits and are catabolized thereafter during ripening. Aconitase, that transforms citrate to isocitrate, is the first step of citric acid catabolism and a major component of the citrate utilization machinery. In this work, the citrus aconitase gene family was first characterized and a phylogenetic analysis was then carried out in order to understand the evolutionary history of this family in plants. Gene expression analyses of the citrus aconitase family were subsequently performed in several acidic and acidless genotypes to elucidate their involvement in acid homeostasis. Results Analysis of 460,000 citrus ESTs, followed by sequencing of complete cDNA clones, identified in citrus 3 transcription units coding for putatively active aconitate hydratase proteins, named as CcAco1, CcAco2 and CcAco3. A phylogenetic study carried on the Aco family in 14 plant species, shows the presence of 5 Aco subfamilies, and that the ancestor of monocot and dicot species shared at least one Aco gene. Real-time RT-PCR expression analyses of the three aconitase citrus genes were performed in pulp tissues along fruit development in acidic and acidless citrus varieties such as mandarins, oranges and lemons. While CcAco3 expression was always low, CcAco1 and CcAco2 genes were generally induced during the rapid phase of fruit growth along with the maximum in acidity and the beginning of the acid reduction. Two exceptions to this general pattern were found: 1) Clemenules mandarin failed inducing CcAco2 although acid levels were rapidly reduced; and 2) the acidless "Sucreña" orange showed unusually high levels of expression of both aconitases, an observation correlating with the acidless phenotype. However, in the acidless "Dulce" lemon aconitase expression was normal suggesting that the acidless trait in this variety is not dependent upon aconitases. Conclusions Phylogenetic studies showed the occurrence of five different subfamilies of aconitate hydratase in plants and sequence analyses indentified three active genes in citrus. The pattern of expression of two of these genes, CcAco1 and CcAco2, was normally associated with the timing of acid content reduction in most genotypes. Two exceptions to this general observation suggest the occurrence of additional regulatory steps of citrate homeostasis in citrus.
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- 2010
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41. Development of genomic resources for Citrus clementina: Characterization of three deep-coverage BAC libraries and analysis of 46,000 BAC end sequences
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Talon Manuel, Ollitrault Patrick, Naranjo M Angel, and Terol Javier
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Citrus species constitute one of the major tree fruit crops of the subtropical regions with great economic importance. However, their peculiar reproductive characteristics, low genetic diversity and the long-term nature of tree breeding mostly impair citrus variety improvement. In woody plants, genomic science holds promise of improvements and in the Citrus genera the development of genomic tools may be crucial for further crop improvements. In this work we report the characterization of three BAC libraries from Clementine (Citrus clementina), one of the most relevant citrus fresh fruit market cultivars, and the analyses of 46.000 BAC end sequences. Clementine is a diploid plant with an estimated haploid genome size of 367 Mb and 2n = 18 chromosomes, which makes feasible the use of genomics tools to boost genetic improvement. Results Three genomic BAC libraries of Citrus clementina were constructed through EcoRI, MboI and HindIII digestions and 56,000 clones, representing an estimated genomic coverage of 19.5 haploid genome-equivalents, were picked. BAC end sequencing (BES) of 28,000 clones produced 28.1 Mb of genomic sequence that allowed the identification of the repetitive fraction (12.5% of the genome) and estimation of gene content (31,000 genes) of this species. BES analyses identified 3,800 SSRs and 6,617 putative SNPs. Comparative genomic studies showed that citrus gene homology and microsyntheny with Populus trichocarpa was rather higher than with Arabidopsis thaliana, a species phylogenetically closer to citrus. Conclusion In this work, we report the characterization of three BAC libraries from C. clementina, and a new set of genomic resources that may be useful for isolation of genes underlying economically important traits, physical mapping and eventually crop improvement in Citrus species. In addition, BAC end sequencing has provided a first insight on the basic structure and organization of the citrus genome and has yielded valuable molecular markers for genetic mapping and cloning of genes of agricultural interest. Paired end sequences also may be very helpful for whole-genome sequencing programs.
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- 2008
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42. Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping
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Ollitrault Patrick, Talon Manuel, Wincker Patrick, Allario Thierry, Argout Xavier, Terol Javier, Costantino Gilles, Luro François L, and Morillon Raphael
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background During the last decade, numerous microsatellite markers were developed for genotyping and to identify closely related plant genotypes. In citrus, previously developed microsatellite markers were arisen from genomic libraries and more often located in non coding DNA sequences. To optimize the use of these EST-SSRs as genetic markers in genome mapping programs and citrus systematic analysis, we have investigated their polymorphism related to the type (di or trinucleotide) or their position in the coding sequences. Results Among 11000 unigenes from a Clementine EST library, we have found at least one microsatellite sequence (repeated units size ranged from 2 to 6 nucleotides) in 1500 unigenes (13.6%). More than 95% of these SSRs were di or trinucleotides. If trinucleotide microsatellites were encountered trough all part of EST sequences, dinucleotide microsatellites were preferentially (50%) concentrated in the 5' 100th nucleotides. We assessed the polymorphism of 41 EST-SSR, by PCR amplification droved with flanking primers among ten Citrus species plus 3 from other genera. More than 90% of EST-SSR markers were polymorphic. Furthermore, dinucleotide microsatellite markers were more polymorphic than trinucleotide ones, probably related to their distribution that was more often located in the 5' UnTranslated Region (UTR). We obtained a good agreement of diversity relationships between the citrus species and relatives assessed with EST-SSR markers with the established taxonomy and phylogeny. To end, the heterozygosity of each genotype and all dual combinations were studied to evaluate the percentage of mappable markers. Higher values (> 45%) were observed for putative Citrus inter-specific hybrids (lime lemon, or sour orange) than for Citrus basic true species (mandarin, pummelo and citron) (70%) with a significant proportion suitable for synteny analysis. Conclusion Fourty one new EST-SSR markers were produced and were available for citrus genetic studies. Whatever the position of the SSR in the ESTs the EST-SSR markers we developed are powerful to investigate genetic diversity and genome mapping in citrus.
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- 2008
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43. Analysis of 13000 unique Citrus clusters associated with fruit quality, production and salinity tolerance
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Dossat Carole, Ollitrault Patrick, Courtois Brigitte, Argout Xavier, Legaz Francisco, Götz Stefan, Iglesias Domingo J, Brumos Javier, Soler Guillermo, Andres Fernando, Alós Enriqueta, Agustí Javier, Tadeo Francisco, Cercos Manuel, Colmenero Jose M, Conesa Ana, Terol Javier, Wincker Patrick, Morillon Raphael, and Talon Manuel
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Improvement of Citrus, the most economically important fruit crop in the world, is extremely slow and inherently costly because of the long-term nature of tree breeding and an unusual combination of reproductive characteristics. Aside from disease resistance, major commercial traits in Citrus are improved fruit quality, higher yield and tolerance to environmental stresses, especially salinity. Results A normalized full length and 9 standard cDNA libraries were generated, representing particular treatments and tissues from selected varieties (Citrus clementina and C. sinensis) and rootstocks (C. reshni, and C. sinenis × Poncirus trifoliata) differing in fruit quality, resistance to abscission, and tolerance to salinity. The goal of this work was to provide a large expressed sequence tag (EST) collection enriched with transcripts related to these well appreciated agronomical traits. Towards this end, more than 54000 ESTs derived from these libraries were analyzed and annotated. Assembly of 52626 useful sequences generated 15664 putative transcription units distributed in 7120 contigs, and 8544 singletons. BLAST annotation produced significant hits for more than 80% of the hypothetical transcription units and suggested that 647 of these might be Citrus specific unigenes. The unigene set, composed of ~13000 putative different transcripts, including more than 5000 novel Citrus genes, was assigned with putative functions based on similarity, GO annotations and protein domains Conclusion Comparative genomics with Arabidopsis revealed the presence of putative conserved orthologs and single copy genes in Citrus and also the occurrence of both gene duplication events and increased number of genes for specific pathways. In addition, phylogenetic analysis performed on the ammonium transporter family and glycosyl transferase family 20 suggested the existence of Citrus paralogs. Analysis of the Citrus gene space showed that the most important metabolic pathways known to affect fruit quality were represented in the unigene set. Overall, the similarity analyses indicated that the sequences of the genes belonging to these varieties and rootstocks were essentially identical, suggesting that the differential behaviour of these species cannot be attributed to major sequence divergences. This Citrus EST assembly contributes both crucial information to discover genes of agronomical interest and tools for genetic and genomic analyses, such as the development of new markers and microarrays.
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- 2007
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44. Continuous flow photocatalytic degradation of carbaryl in aqueous media
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Pramauro, E., de la Guardia, M., Mauri, A. R., Terol, J., and Peris-Cardells, E.
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WATER quality ,CARBARYL - Published
- 1993
45. Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization
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Gerardo Llácer, Gracia Martí, María Luisa Badenes, Manuel Agustí, Gabino Ríos, Javier Terol, Carmen Leida, Leida C., Terol J., Marti G., Agusti M., Llacer G., Badenes M.L., and Rios G.
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Genetics ,Microarray ,Physiology ,Bud ,Plant Science ,Biology ,Peach ,Flower bud ,Suppression subtractive hybridization ,Complementary DNA ,Gene expression ,Dormancy ,Hybridization, Genetic ,Prunu ,Season ,Prunus ,Seasons ,SSH ,Transcription factor ,DNA microarray ,Gene ,Chilling - Abstract
To better understand the molecular and physiological mechanisms underlying maintenance and release of seasonal bud dormancy in perennial trees, we identified differentially expressed genes during dormancy progression in reproductive buds from peach (Prunus persica [L.] Batsch) by suppression subtractive hybridization (SSH) and microarray hybridization. Four SSH libraries were constructed, which were respectively enriched in cDNA highly expressed in dormant buds (named DR), in dormancy-released buds (RD) and in the cultivars with different chilling requirement, 'Zincal 5' (ZS) and 'Springlady' (SZ), sampled after dormancy release. About 2500 clones picked from the four libraries were loaded on a glass microarray. Hybridization of microarrays with the final products of SSH procedure was performed in order to validate the selected clones that were effectively enriched in their respective sample. Nearly 400 positive clones were sequenced, which corresponded to 101 different unigenes with diverse functional annotation. We obtained DAM4, 5 and 6 genes coding for MADS-box transcription factors previously related to growth cessation and terminal bud formation in the evergrowing mutant of peach. Several other cDNAs are similar to dormancy factors described in other species, and others have been related to bud dormancy for the first time in this study. Quantitative reverse transcription polymerase chain reaction analysis confirmed differential expression of cDNAs coding for a Zn-finger transcription factor, a GRAS-like regulator, a DNA-binding protein and proteins similar to forisome subunits involved in the reversible occlusion of sieve elements in Fabaceae, among others. © The Author 2010. Published by Oxford University Press. All rights reserved.
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- 2010
46. Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana
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Jean Weissenbach, William C. Nierman, Christopher D. Town, A Perez-Perez, R. Cooke, Brian J. Haas, Samir Kaul, T Kato, Claire Fujii, J Militscher, Mitsuyo Kohara, Steven L. Salzberg, A Conrad, Hans-Werner Mewes, D. Haase, M. Scharfe, S Bangert, Hean L. Koo, W. Ansorge, Laurence Cattolico, Patrick Wincker, Rama Maiti, Marcel Salanoubat, Erika Asamizu, Bénédicte Purnelle, Luke J. Tallon, M flores, Grace Pai, P Brottier, Kumi Idesawa, Richard Holland, P Sellers, J C Venter, S Nakayama, Michela D'Angelo, Holger Erfle, Berthold Fartmann, Ai Matsuno, Elena Casacuberta, Barbara Simionati, T Wada, R Wiedelmann, Amparo Monfort, Chiaki Kiyokawa, M. Rizzo, Jeremy Peterson, D. Vitale, Joan Climent, M. Schäfer, C Takeuchi, Gertrud Mannhaupt, Terrance Shea, P Navarro, Gerald Nyakatura, Pere Puigdomènech, R Mache, Leslie A. Grivell, S. van Aken, Paolo Zaccaria, Stephen Rudd, H. Voss, B Ottenwälder, Todd Creasy, J Reichelt, C Berger-Llauro, M Laudie, K Hornischer, H Drzonek, J P Alcaraz, Kai Lemcke, M Unseld, N Jordan, C Robert, Shusei Sato, T Kimura, S Müller-Auer, Naomi Nakazaki, W Saurin, Daphne Preuss, M. de Haan, J Jenkins, Francis Quetier, D Duchemin, Xiaoying Lin, Alberto Pallavicini, A Watanabe, Petra Brandt, Klaus F. X. Mayer, Heiko Schoof, M Yamada, Javier Terol, Satoshi Tabata, Benes, John Gill, François Artiguenave, Yoshie Kishida, Nathalie Choisne, O Schön, C. Gabel, E Wurmbach, Michael A. Rieger, Alessandro Vezzi, T Kaneko, T. H. Löhnert, Owen White, G Kauer, M Matsumoto, M. Fuchs, A Walts, G Nordsiek, Michel Delseny, Shigemi Sasamoto, H Kranz, Rosario Liguori, Yasukazu Nakamura, David Masuy, H. Blöcker, De Simone, Miho Yasuda, Tamara Feldblyum, B. Obermaier, Giorgio Valle, Manuel Pérez-Alonso, Sayaka Shinpo, Kumiko Kawashima, A Cottet, Anagnostis Argiriou, T Rooney, A.C. Maarse, Dongying Wu, C Collado, T. Utterback, Claire M. Fraser, M. D. Bargues, Stefano Toppo, Marc Boutry, Akiko Muraki, Salanoubat, M., Lemcke, K., Rieger, M., Ansorge, W., Unseld, M., Fartmann, B., Valle, G., Blocker, H., Perezalonso, M., Obermaier, B., Delseny, M., Boutry, M., Grivell, L. A., Mache, R., Puigdomenech, P., DE SIMONE, V., Choisne, N., Artiguenave, F., Robert, C., Brottier, P., Wincker, P., Cattolico, L., Weissenbach, J., Saurin, W., Quetier, F., Schafer, M., Mullerauer, S., Gabel, C., Fuchs, M., Benes, V., Wurmbach, E., Drzonek, H., Erfle, H., Jordan, N., Bangert, S., Wiedelmann, R., Kranz, H., Voss, H., Holland, R., Brandt, P., Nyakatura, G., Vezzi, A., D'Angelo, M., Pallavicini, Alberto, Toppo, S., Simionati, B., Conrad, A., Hornischer, K., Kauer, G., Lohnert, T. H., Nordsiek, G., Reichelt, J., Scharfe, M., Schon, O., Bargues, M., Terol, J., Climent, J., Navarro, P., Collado, C., Perezperez, A., Ottenwalder, B., Duchemin, D., Cooke, R., Laudie, M., Bergerllauro, C., Purnelle, B., Masuy, D., DE HAAN, M., Maarse, A. C., Alcaraz, J. P., Cottet, A., Casacuberta, E., Monfort, A., Argiriou, A., Flores, M., Liguori, R., Vitale, D., Mannhaupt, G., Haase, D., and Schoof, H.
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DNA, Plant ,Sequence analysis ,Arabidopsis ,plant ,Genome ,Complete sequence ,Gene Duplication ,Centromere ,Plant genomics ,model organism ,Humans ,genomic structure ,Gene ,Plant Proteins ,Genetics ,Multidisciplinary ,biology ,Chromosome ,Chromosome Mapping ,Sequence Analysis, DNA ,biology.organism_classification ,genome sequencing ,Chromosome 3 ,Genome, Plant - Abstract
Arabidopsis thaliana is an important model system for plant biologists. In 1996 an international collaboration (the Arabidopsis Genome Initiative) was formed to sequence the whole genome of Arabidopsis and in 1999 the sequence of the first two chromosomes was reported. The sequence of the last three chromosomes and an analysis of the whole genome are reported in this issue. Here we present the sequence of chromosome 3, organized into four sequence segments (contigs). The two largest (13.5 and 9.2 Mb) correspond to the top (long) and the bottom (short) arms of chromosome 3, and the two small contigs are located in the genetically defined centromere. This chromosome encodes 5,220 of the roughly 25,500 predicted protein-coding genes in the genome. About 20% of the predicted proteins have significant homology to proteins in eukaryotic genomes for which the complete sequence is available, pointing to important conserved cellular functions among eukaryotes.
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- 2000
47. TRPC5 controls the adrenaline-mediated counter regulation of hypoglycemia.
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Bröker-Lai J, Rego Terol J, Richter C, Mathar I, Wirth A, Kopf S, Moreno-Pérez A, Büttner M, Tan LL, Makke M, Poschet G, Hermann J, Tsvilovskyy V, Haberkorn U, Wartenberg P, Susperreguy S, Berlin M, Ottenheijm R, Philippaert K, Wu M, Wiedemann T, Herzig S, Belkacemi A, Levinson RT, Agarwal N, Camacho Londoño JE, Klebl B, Dinkel K, Zufall F, Nussbaumer P, Boehm U, Hell R, Nawroth P, Birnbaumer L, Leinders-Zufall T, Kuner R, Zorn M, Bruns D, Schwarz Y, and Freichel M
- Abstract
Hypoglycemia triggers autonomic and endocrine counter-regulatory responses to restore glucose homeostasis, a response that is impaired in patients with diabetes and its long-term complication hypoglycemia-associated autonomic failure (HAAF). We show that insulin-evoked hypoglycemia is severely aggravated in mice lacking the cation channel proteins TRPC1, TRPC4, TRPC5, and TRPC6, which cannot be explained by alterations in glucagon or glucocorticoid action. By using various TRPC compound knockout mouse lines, we pinpointed the failure in sympathetic counter-regulation to the lack of the TRPC5 channel subtype in adrenal chromaffin cells, which prevents proper adrenaline rise in blood plasma. Using electrophysiological analyses, we delineate a previously unknown signaling pathway in which stimulation of PAC1 or muscarinic receptors activates TRPC5 channels in a phospholipase-C-dependent manner to induce sustained adrenaline secretion as a crucial step in the sympathetic counter response to insulin-induced hypoglycemia. By comparing metabolites in the plasma, we identified reduced taurine levels after hypoglycemia induction as a commonality in TRPC5-deficient mice and HAAF patients., (© 2024. The Author(s).)
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- 2024
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48. Comparative transcriptomic analyses of citrus cold-resistant vs. sensitive rootstocks might suggest a relevant role of ABA signaling in triggering cold scion adaption.
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Primo-Capella A, Forner-Giner MÁ, Martínez-Cuenca MR, and Terol J
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- Hormones metabolism, Plant Roots genetics, Plant Roots metabolism, Transcriptome, Citrus metabolism, Citrus sinensis genetics
- Abstract
Background: The citrus genus comprises a number of sensitive tropical and subtropical species to cold stress, which limits global citrus distribution to certain latitudes and causes major economic loss. We used RNA-Seq technology to analyze changes in the transcriptome of Valencia delta seedless orange in response to long-term cold stress grafted on two frequently used citrus rootstocks: Carrizo citrange (CAR), considered one of the most cold-tolerant accessions; C. macrophylla (MAC), a very sensitive one. Our objectives were to identify the genetic mechanism that produce the tolerant or sensitive phenotypes in citrus, as well as to gain insights of the rootstock-scion interactions that induce the cold tolerance or sensitivity in the scion., Results: Plants were kept at 1 ºC for 30 days. Samples were taken at 0, 15 and 30 days. The metabolomic analysis showed a significant increase in the concentration of free sugars and proline, which was higher for the CAR plants. Hormone quantification in roots showed a substantially increased ABA concentration during cold exposure in the CAR roots, which was not observed in MAC. Different approaches were followed to analyze gene expression. During the stress treatment, the 0-15-day comparison yielded the most DEGs. The functional characterization of DEGs showed enrichment in GO terms and KEGG pathways related to abiotic stress responses previously described in plant cold adaption. The DEGs analysis revealed that several key genes promoting cold adaption were up-regulated in the CAR plants, and those repressing it had higher expression levels in the MAC samples., Conclusions: The metabolomic and transcriptomic study herein performed indicates that the mechanisms activated in plants shortly after cold exposure remain active in the long term. Both the hormone quantification and differential expression analysis suggest that ABA signaling might play a relevant role in promoting the cold hardiness or sensitiveness of Valencia sweet orange grafted onto Carrizo citrange or Macrophylla rootstocks, respectively. Our work provides new insights into the mechanisms by which rootstocks modulate resistance to abiotic stress in the production variety grafted onto them., (© 2022. The Author(s).)
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- 2022
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49. Comparative transcriptomics of wild and commercial Citrus during early ripening reveals how domestication shaped fruit gene expression.
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Borredá C, Perez-Roman E, Talon M, and Terol J
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- Domestication, Fruit genetics, Fruit metabolism, Transcriptome, Citrus genetics, Citrus metabolism, Citrus sinensis genetics
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Background: Interspecific hybridizations and admixtures were key in Citrus domestication, but very little is known about their impact at the transcriptomic level. To determine the effects of genome introgressions on gene expression, the transcriptomes of the pulp and flavedo of three pure species (citron, pure mandarin and pummelo) and four derived domesticated genetic admixtures (sour orange, sweet orange, lemon and domesticated mandarin) have been analyzed at color break., Results: Many genes involved in relevant physiological processes for domestication, such sugar/acid metabolism and carotenoid/flavonoid synthesis, were differentially expressed among samples. In the low-sugar, highly acidic species lemon and citron, many genes involved in sugar metabolism, the TCA cycle and GABA shunt displayed a reduced expression, while the P-type ATPase CitPH5 and most subunits of the vacuolar ATPase were overexpressed. The red-colored species and admixtures were generally characterized by the overexpression in the flavedo of specific pivotal genes involved in the carotenoid biosynthesis, including phytoene synthase, ζ-carotene desaturase, β-lycopene cyclase and CCD4b, a carotenoid cleavage dioxygenase. The expression patterns of many genes involved in flavonoid modifications, especially the flavonoid and phenylpropanoid O-methyltransferases showed extreme diversity. However, the most noticeable differential expression was shown by a chalcone synthase gene, which catalyzes a key step in the biosynthesis of flavonoids. This chalcone synthase was exclusively expressed in mandarins and their admixed species, which only expressed the mandarin allele. In addition, comparisons between wild and domesticated mandarins revealed that the major differences between their transcriptomes concentrate in the admixed regions., Conclusion: In this work we present a first study providing broad evidence that the genome introgressions that took place during citrus domestication largely shaped gene expression in their fruits., (© 2022. The Author(s).)
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- 2022
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50. Shaping the biology of citrus: I. Genomic determinants of evolution.
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Gonzalez-Ibeas D, Ibanez V, Perez-Roman E, Borredá C, Terol J, and Talon M
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- Flavonoids, Genomics, Citrus chemistry, Citrus genetics
- Abstract
We performed genomic analyses on wild species of the genus Citrus to identify major determinants of evolution. The most notable effect occurred on the pathogen-defense genes, as observed in many other plant genera. The gene space was also characterized by changes in gene families intimately related to relevant biochemical properties of citrus fruit, such as pectin modifying enzymes, HDR (4-hydroxy-3-methylbut-2-enyl diphosphate reductase) genes, and O-methyltransferases. Citrus fruits are highly abundant on pectins and secondary metabolites such as terpenoids and flavonoids, the targets of these families. Other gene types under positive selection, expanded through tandem duplications and retained as triplets from whole genome duplications, codified for purple acid phosphatases and MATE-efflux proteins. Although speciation has not been especially rapid in the genus, analyses of selective pressure at the codon level revealed that the extant species evolved from the ancestral citrus radiation show signatures of pervasive adaptive evolution and is therefore potentially responsible for the vast phenotypic differences observed among current species., (© 2021 The Authors. The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America.)
- Published
- 2021
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