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В статье рассматриваются методики составления прогнозов результатов выборов на основе опросных данных. В основе большинства таких прогнозов лежит методика, сконструированная Дж. Гэллапом в середине прошлого века. Авторы прослеживают эволюцию модели Дж. Гэллапа от хрестоматийной «победы 1936 года» до современных модификаций. Описаны эксперименты, проведенные в ходе формирования прогнозных моделей, представлен перечень вопросов, используемых американскими полстерами для прогнозирования результатов выборов, подробно разобран механизм подсчета баллов в моделях отсечения (cutoff), рассмотрены ограничения модели Гэллапа.

An extracellular nuclease was purified 165-fold with a specific activity of 41,250 U/mg poly(U) by chromatography with modified chitosan from the culture of marine fungus Penicillium melinii isolated from colonial ascidium collected near Shikotan Island, Sea of Okhotsk, at a depth of 123 m. The purified nuclease is a monomer with the molecular weight of 35 kDa. The enzyme exhibits maximum activity at pH 3.7 for DNA and RNA. The enzyme is stable until 75°C and in the pH range of 2.5-8.0. The enzyme endonucleolytically degrades ssDNA and RNA by 3'-5' mode to produce 5'-oligonucleotides and 5'-mononucleotides; however, it preferentially degrades poly(U). The enzyme can digest dsDNA in the presence of pregnancy-specific beta-1-glycoprotein-1. The nuclease acts on closed circular double-stranded DNA to produce opened circular DNA and then the linear form DNA by single-strand scission. DNA sequence encoding the marine fungus P. melinii endonuclease revealed homology to S1-type nucleases. The tight correlation found between the extracellular endonuclease activity and the rate of H³-thymidine uptake by actively growing P. melinii cells suggests that this nuclease is required for fulfilling the nucleotide pool of precursors of DNA biosynthesis during the transformation of hyphae into the aerial mycelium and conidia in stressful environmental conditions.

Thymidilate kinase (EC 2.7.4.9, ATP:dTMP phosphotransferase) was isolated from eggs of the sea urchin Strongylocentrotus intermedius. The enzyme preparation was purified by 1073-fold and was not contaminated with phosphatase or ATPase. The molecular weight of the sea urchin thymidilate kinase is 100 kD, and the pH optimum of its action is 8-8.5. The thymidilate kinase activity is maximal in the presence of 2-5 mM ATP and 10 mM MgCl2. In the reaction of phosphorylation with dTMP, Mg2+ can be partially substituted by other bivalent metal ions whose efficiency decreases in the series: Mg2+ > Mn2+ > Ca2+ = Cd2+ = Co2+. In the presence of Zn2+, Fe2+, Cu2+, and Pb2+ the thymidilate kinase is inactive. The sea urchin thymidilate kinase can utilize ATP, dCTP, and dTTP as donors of the phosphate group. Either dTMP or dCMP can serve as the acceptor of phosphate. Addition of thymidine and other nucleosides to the reaction medium has virtually no effect on the rate of dTMP phosphorylation.