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This study aimed to evaluate biomarkers of oxidative stress (2-thiobarbituric acid reactive substances, aldehyde and ketone derivatives of oxidatively modified proteins and total antioxidant capacity), the activity of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase), that of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase and acid phosphatase) and changes in biochemical parameters (alanine aminotransferase, aspartate aminotransferase, de Ritis ratio, lactate dehydrogenase activity, lactate and pyruvate levels and their ratio) in the liver tissue of fish that were vaccinated against enteric redmouth disease and challenged with its causative agent, the bacterium Yersinia ruckeri.

This study analyzed time-dependent effects of vaccination against Y. ruckeri on the oxidative mechanism underlying those effects by detecting relevant lipid peroxidation (2-thiobarbituric acid reactive substances, TBARS) and protein oxidation biomarkers [aldehydic and ketonic derivatives of oxidatively modified proteins (OMP)], antioxidant defenses [activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), total antioxidant capacity (TAC)], as well as activities of lysosomal functioning [alanyl aminopeptidase (AAP), leucyl aminopeptidase (LAP), acid phosphatase (AcP), and â-N-acetylglucosaminidase (NAG)] in hepatic tissue of rainbow trout, Oncorhynchus mykiss (Walbaum) following anti-Y. ruckeri vaccination in the first, second, and sixth months. A concentrated vaccine with Y. ruckeri strains was enclosed in fish feed and was administered three times every other day. Rainbow trout from each group were euthanized 31, 61, and 181 days following vaccination, and hepatic tissue was sampled for analysis. In the current study, vaccination against Y. ruckeri resulted in a no statistically significant change in TBARS levels, while aldehydic and ketonic derivatives of OMP in hepatic tissue decreased, especially after the first and second months following immunization. Moreover, the activities of glutathione-dependent enzymes increased, especially after the first and sixth months. The highest TAC levels were observed two and six months after vaccination. It has been shown that vaccination-related oxidative stress in hepatic tissue is involved in adaptive responses through the temporary mobilization of antioxidant and lysosomal enzymes in rainbow trout. The present study showed the effect of vaccination on lysosome membrane permeability for carbohydrate cleavage after the development of immunity against Yersinia, whereas antioxidant defence was reduced. Our results confirmed that the concept of preserving antioxidant enzyme function after vaccination was also evident when CAT, GR, and GPx activities either increased or were unchanged following vaccination.

Juvenile sterlet (Acipenser ruthenus) were fed commercial feed (group C) and experimental feed supplemented with papain in doses of 10 g kg−1 feed (P1) and 20 g kg−1 feed (P2) for eight weeks. Growth, digestive enzyme activity, immunity parameters, pathological changes of the liver and intestine, proximate body composition, and oxidative response were assayed. A challenge test with Yersinia ruckeri was also performed. The final body weights of groups P1 (107.07 ± 7.66 g) and P2 (111.98 ± 1.93 g) were significantly (P

The objective of this study was to investigate the effect of trans-resveratrol, a potent antioxidant with anti-inflammatory and chemopreventive properties, naturally occurring in many fruits and plants on lymphocytes proliferation and also on macrophages metabolic and phagocytic activity. The aim of this study was to demonstrate the immunomodulatory effects of the compound on fish immunocompetent cells and determine the type of this interaction (immunosuppression or immunostimulation). Proliferative activity of lymphocytes was studied by MTT assay, and the respiratory burst was evaluated using the respiratory burst activity (RBA) test. Phagocytic killing was tested using the PKA test. The experiment have shown that trans-resveratrol suppressed blood B cells, while there was no significant influence on blood T lymphocytes. However, insignificant stimulatory effect occurred at the lowest concentration. In addition, the compound inhibited proliferation of T and B lymphocytes isolated from the organs. Importantly, trans-resveratrol caused stimulation of blood and organs macrophages phagocytic killing, and also increased the respiratory burst of macrophages isolated from organ. These results suggest a potential use of trans-resveratrol as an immunomodulator of innate immunity in fish. This is particularly important, as this kind of resistance plays leading role in protecting the body against infection. In comparison, adaptive immunity is slower and also much less precise.

The goal of the research was to select measures of regional competitiveness and analyze if in the considered period changes in value of these measures were in line with changes in the debt level of different forms of municipalities (communes). The survey was conducted for the Lower Silesia region and the municipalities located there. Data covered the period 2005-2015. The main measure of regional competitiveness selected by the author was regional GDP per capita. The additional measures were: labour productivity, employment rate, average monthly salary and business density. The survey indicated that the value of communes debt in Lower Silesia increased, even if in some years for particular forms of communes the level of debt was decreasing. At the same time generally an increase of competitiveness measures in the region was observed. In most of the analyzed years the direction of changes in municipalities debt was the same as that of changes of regional competitiveness measures.

Bacterial diseases are a significant problem in the controlled rearing of fish. Furunculosis (Aeromonas sp.), flavobacteriosis (Flavobacterium sp.), and pseudomonadosis (Pseudomonas sp.) are currently the most frequently identified diseases in recirculating aquaculture systems of various fish species. Such a situation is also observed in pikeperch rearing. Due to the emerging difficulties of effective prophylaxis using commercial vaccines, interest in the use of autovaccinations is increasing, not only in ichthyopathology but also in other veterinary fields. Our research aimed to assess the effect of the vaccination method on the overall condition of the fish and survival after the experimental infection with Aeromonas salmonicida. Pikeperch were vaccinated by (1) bath, (2) a single i.p. injection, or (3) feed. The fish were measured and weighed on day 0 and after 28 and 56 days of the experiment. Specific growth rate, daily growth rate, condition factor, and feed conversion ratio were calculated. On days 7, 14, 21, and 28 of the experiment, ceruloplasmin and lysozyme levels were rated. In addition, a challenge test was performed. The obtained results showed that the method of vaccination is important and affects the growth of fish, the overall condition of fish, and survival after experimental infection.

This study analysed the effect of the type of production on the nutritional and health value of common carp muscle tissue, especially lipid-related indexes. The muscle tissue of common carps originating from three types of fish farms with different technological levels (i.e., intensive, low-intensive, and semiextensive) was studied. The type of production technology, especially the intensive breeding technique, has been shown to have a significant effect on dry weight, total protein, and total fat content in the common carp muscle tissue under study. The muscles of fish originating from these farms were characterised by the highest values of the mentioned indicators, which amounted to 24.6%, 18.74%, and 1.58%, respectively, for dry matter, protein content, and total fat content. The type of feed used in intensive, low-intensive, and semiextensive common carp breeding has been proven to have a highly significant effect on the fatty acid profile. As regards unsaturated fatty acids, monounsaturated fatty acids were dominant in all cases. The muscle tissue of fish cultured in farms with a semiextensive technological level was characterised by the highest content of unsaturated fatty acids. Moreover, the ratio of polyunsaturated fatty acids to saturated fatty acids was, in this case, the most favourable. Dietary indices of atherogenicity (AI) and thrombogenicity (TI) were studied as well. In all analysed cases, the values of these indices were very favourable and several times lower than for other animal fats.

Objective. To examine general mental health in adult males and females, who in adolescence participated in a scoliosis-specific therapeutic exercise program or were under observation due to diagnosis of scoliosis. Design. Registry-based, cross-sectional study with retrospective data collection. Methods. Sixty-eight subjects (43 women) aged 30.10 (25–39) years, with mild or moderate scoliosis (11–36° Cobb angle), and 76 (38 women) nonscoliotic subjects, aged 30.11 (24–38) years, participated. The time period since the end of the exercise or observation regimes was 16.5 (12-26) years. Beck Depression Inventory (BDI) and General Health Questionnaire (GHQ-28) scores were analyzed with the χ2 and U tests. Multiple regression analyses for confounders were also performed. Results. Intergroup differences of demographic characteristics were nonsignificant. Scoliosis, gender, participation in the exercise program, employment, and marital status were associated with BDI scores. The presence of scoliosis and participation in the exercise program manifested association with the symptoms. Higher GHQ-28 “somatic symptoms” subscale scores interacted with the education level. Conclusions. Our findings correspond to the reports of a negative impact of the diagnosis of scoliosis and treatment on mental health. The decision to introduce a therapeutic program in children with mild deformities should be made with judgment of potential benefits, risks, and harm.

The purpose of this study was to evaluate the quality of nursing care of postoperative pain management in patients who underwent vascular procedures. A survey using patient questionnaires. The sample was composed of 100 patients, aged 52 to 86 years, admitted to the Clinic of Cardiac and Vascular Surgery, University Clinical Centre, Gdansk, Poland. The study included the use of an interview questionnaire, that is,.e. a standardized research tool—the Strategic and Clinical Quality Indicators in Postoperative Pain Management scale and a questionnaire designed to record sociodemographic characteristics. Analysis of the data indicated that the most numerous group of patients were men (80%). The total mean score obtained with the Strategic and Clinical Quality Indicators in Postoperative Pain Management scale was 59.2 points (range, 14 to 70) meaning that the high quality of nursing care in each area was not provided. The results of this research revealed areas for improvement in postoperative pain management on the subscales: communication (12.9 points), action (15.3 points), and environment (12.5 points). The overall level of quality of nursing care in terms of postoperative pain management as reported by patients after vascular procedures was not fully satisfying, and nursing care should improve. This indicates the need to educate nurses in postoperative pain management, its monitoring and prophylaxis, and communication techniques with patients. Our study indicates that gender, place of residence, education level, and satisfaction with pain management influence the assessment of the quality of care. [ABSTRACT FROM AUTHOR]

The crystallographic structure of a steroid derivative molecule, 17,17-di-n-propyl-17a-aza-D-homo-5alpha-androstan-3beta-ol is obtained from a single crystal by laboratory X-ray and from a polycrystalline powder by high-resolution synchrotron powder diffraction. The molecule crystallizes in the orthorhombic space group P[2.sub.1][2.sub.1][2.sub.1] (a = 6.5346, b = 17.6006 and C = 19.6978 Angstrom) and the hydrogen bonds from infinite chains of molecules parallel to the c axis.

Aim: Case based evaluation of hyperbaric oxygen therapy in combination with negative pressure therapy and standard methods of wound treatment, to improve the healing of an extensive wound after radiotherapy. Method: A 51-year-old Caucasian woman after whole breast radiotherapy due to right breast cancer. Within a year of oncological treatment, patient developed the full-thickness, radiation induced skin necrosis in the irradiated area. Due to the non-healing chronic wound, the patient was repeatedly consulted by general practitioners, however the recommended local treatment did not bring any improvement. Finally, patient with an extensive chronic wound, was admitted to the hospital for the long-lasting individualised treatment. The patient underwent a multi-modal and multi-step treatment including hyperbaric therapy (37 -- sessions), surgical methods, negative pressure therapy (40-60 mmHg), sonotherapy (20 -- sessions) and personalized nutritional and analgesic treatment. Results / Discussion: In presented case, after 8 months of hospitalization, the process of wound treatment was completed with surgical transposition of skin-muscle flaps to reconstruct chest wall defects. The patient was discharged home and after another 3 months of observation, complete healing of the extensive chest radiation wound was achieved. Conclusion: A non-healing, deteriorating wound after radiotherapy requires treatment in highly specialized centres with highly qualified personnel and various, modern methods of wound management. The treatment of an extensive radiation damage to the skin requires long-term local therapy. Therefore, it is worth to remember that the use of hyperbaric oxygen therapy with negative pressure therapy as well as nutritional support and effective analgesic treatment in similar cases may bring positive effects in the management of the radiation-induced skin necrosis. [ABSTRACT FROM AUTHOR]

In contrast to terrestrial animals, fish can ingest minerals from food or directly from water. Although micro- and macro-elements are needed in small quantities, they play a key role in many metabolic processes. Trace mineral and macromineral deficiencies may go unnoticed due to an absence of clear clinical symptoms in fish. Absorption processes are determined by various factors, mostly mineral concentrations in water but also other water parameters. The required dietary supplementation of macronutrients and micronutrients is very difficult to determine, and the amount of nutrients absorbed by fish from water is equally difficult to measure. Interactions between elements should also be taken into consideration. Many authors emphasize that phosphates may reduce the absorption of most micronutrients. Also, the current parameters of the water can affect the bioavailability. Some elements such as calcium, chlorine and sodium can be absorbed from ambient water in a quantity sufficient to meet the demand for this element. Other elements, however, require supplementation in a diet. For example, studies indicate the need for supplementation of phosphorus, zinc, copper and manganese. Most research concentrates on feedstuff as a source of micro- and macronutrients. Meanwhile, information concerning bioavailability of minerals directly from water is scarce. The aim of this study was to analyse literature from a different perspective, and concentrate on water as a source of minerals in fish nutrition. Measurements of water parameters such as temperature, pH, nitrate and nitrite levels and the amount of dissolved oxygen are a regular component of environmental control in fish farming. Determination of micro- and macro-element levels, however, remains uncommon in aquaculture. Measurements of these parameters could suggest which elements need to be supplemented and which are found in water in amounts that satisfy the needs of the fish. [ABSTRACT FROM AUTHOR]