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18 results on '"Temma, Kenta"'

4. Selective plane activation structured illumination microscopy

Author

Temma, Kenta, primary, Oketani, Ryosuke, additional, Kubo, Toshiki, additional, Bando, Kazuki, additional, Maeda, Shunsuke, additional, Sugiura, Kazunori, additional, Matsuda, Tomoki, additional, Heintzmann, Rainer, additional, Kaminishi, Tatsuya, additional, Fukuda, Koki, additional, Hamasaki, Maho, additional, Nagai, Takeharu, additional, and Fujita, Katsumasa, additional

Published
2023
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12. Using saturated absorption for superresolution laser scanning transmission microscopy.

Author

Nishida, Kentaro, Sato, Hikaru, Oketani, Ryosuke, Mochizuki, Kentaro, Temma, Kenta, Kumamoto, Yasuaki, Tanaka, Hideo, and Fujita, Katsumasa

Subjects
MICROSCOPY, ABSORPTION, LASERS, NUMERICAL calculations, SPATIAL resolution, HIGH-intensity focused ultrasound
Abstract

Copyright of Journal of Microscopy is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2022
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16. Hyperspectral two-photon excitation microscopy using visible wavelength

Author

Kubo, Toshiki, Temma, Kenta, Smith, Nicholas I., Lu, Kai, Matsuda, Tomoki, Nagai, Takeharu, and Fujita, Katsumasa

Abstract

We demonstrate hyperspectral imaging by visible-wavelength two-photon excitation microscopy using line illumination and slit-confocal detection. A femtosecond pulsed laser light at 530 nm was used for the simultaneous excitation of fluorescent proteins with different emission wavelengths. The use of line illumination enabled efficient detection of hyperspectral images and achieved simultaneous detection of three fluorescence spectra in the observation of living HeLa cells with an exposure time of 1 ms per line, which is equivalent to about 2 µs per pixel in point scanning, with 160 data points per spectrum. On combining linear spectral unmixing techniques, localization of fluorescent probes in the cells was achieved. A theoretical investigation of the imaging property revealed high-depth discrimination property attained through the combination of nonlinear excitation and slit detection.

Published
2021

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