Search

Your search keyword '"Temkin, Elizabeth"' showing total 217 results
Start Over Author "Temkin, Elizabeth"
217 results on '"Temkin, Elizabeth"'

3. In vivo fitness of carbapenem-resistant Acinetobacter baumannii strains in murine infection is associated with treatment failure in human infections

Author

Paul, Mical, Benattar, Yael Dishon, Dickstein, Yaakov, Bitterman, Roni, Zayyad, Hiba, Koppel, Fidi, Zak-Doron, Yael, Altunin, Sergey, Andria, Nizar, Neuberger, Ami, Stern, Anat, Petersiel, Neta, Raines, Marina, Karban, Amir, Leibovici, Leonard, Yahav, Dafna, Eliakim-Raz, Noa, Zusman, Oren, Elbaz, Michal, Atamna, Heyam, Daitch, Vered, Babich, Tanya, Carmeli, Yehuda, Nutman, Amir, Adler, Amos, Levi, Inbar, Daikos, George L., Skiada, Anna, Pavleas, Ioannis, Antoniadou, Anastasia, Kotsaki, Antigoni, Durante-Mangoni, Emanuele, Andini, Roberto, Iossa, Domenico, Bernardo, Mariano, Cavezza, Giusi, Bertolino, Lorenzo, Giuffre, Giuseppe, Giurazza, Roberto, Ruocco, Giuseppe, Galdo, Maria, Murino, Patrizia, Cristinziano, Adriano, Corcione, Antonio, Zampino, Rosa, Mouton, Johan, Friberg, Lena, Theuretzbacher, Ursula, Temkin, Elizabeth, Lellouche, Jonathan, Rakovitsky, Nadya, Hameir, Amichay, Daikos, George, Dishon, Yael, Eliakim, Noa, and Friberg, Lena E.

Published
2022
Full Text
View/download PDF

7. The Natural History of Carbapenemase-Producing Enterobacterales: Progression From Carriage of Various Carbapenemases to Bloodstream Infection.

Author

Temkin, Elizabeth, Solter, Ester, Lugassy, Carmela, Chen, Dafna, Cohen, Adi, Schwaber, Mitchell J, Carmeli, Yehuda, and Group, CPE Working

Subjects
*RISK assessment, *CARRIER state (Communicable diseases), *ENTEROBACTERIACEAE diseases, *MICROBIAL sensitivity tests, *BACTEREMIA, *DRUG resistance in microorganisms, *RETROSPECTIVE studies, *DESCRIPTIVE statistics, *ONCOLOGY, *LONGITUDINAL method, *HEMATOLOGY, *ENTEROBACTERIACEAE, *INTENSIVE care units, *CARBAPENEM-resistant bacteria, *MEDICAL screening, *CONFIDENCE intervals, *BETA lactamases, *DISEASE progression, *KLEBSIELLA, *HOSPITAL wards, *DISEASE complications, MORTALITY risk factors
Abstract

Background Little is known about the risk of progression from carbapenemase-producing Enterobacterales (CPE) carriage to CPE bloodstream infection (BSI) outside of high-risk settings. We aimed to determine the incidence of CPE BSI among CPE carriers and to assess whether the incidence differs by carbapenemase, species, and setting. Methods We conducted a nationwide population-based retrospective cohort study using national databases. The cohort consisted of all patients in Israel with CPE detected by screening from 1 January 2020 to 10 October 2022. We calculated the cumulative incidence of CPE BSI within 1 year among CPE carriers. We used a competing-risks model with BSI as the outcome and death as the competing risk. Results The study included 6828 CPE carriers. The cumulative incidence of CPE BSI was 2.4% (95% confidence interval [CI], 2.1–2.8). Compared with Klebsiella pneumoniae carbapenemase (KPC), the subhazard of BSI was lower for New Delhi metallo-β-lactamase (NDM) (adjusted subhazard ratio [aSHR], 0.72; 95% CI,.49–1.05) and oxacillinase-48-like (OXA-48-like) (aSHR, 0.60; 95% CI,.32–1.12) but these differences did not reach statistical significance. Compared with K. pneumoniae , the subhazard of BSI was lower for carriers of carbapenemase-producing Escherichia coli (aSHR, 0.33; 95% CI,.21–.52). The subhazard of BSI was higher among patients with CPE carriage first detected in intensive care units (aSHR, 2.10; 95% CI, 1.27–3.49) or oncology/hematology wards (aSHR, 3.95; 95% CI, 2.51–6.22) compared with medical wards. Conclusions The risk of CPE BSI among CPE carriers is lower than previously reported in studies that focused on high-risk patients and settings. The risk of BSI differs significantly by bacterial species and setting, but not by carbapenemase. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

8. Colistin plus meropenem for carbapenem-resistant Gram-negative infections: in vitro synergism is not associated with better clinical outcomes

Author

Paul, Mical, Benattar, Yael Dishon, Dickstein, Yaakov, Bitterman, Roni, Zayyad, Hiba, Koppel, Fidi, Zak-Doron, Yael, Altunin, Sergey, Andria, Nizar, Neuberger, Ami, Stern, Anat, Petersiel, Neta, Raines, Marina, Karban, Amir, Leibovici, Leonard, Yahav, Dafna, Eliakim-Raz, Noa, Zusman, Oren, Elbaz, Michal, Atamna, Heyam, Daitch, Vered, Babich, Tanya, Carmeli, Yehuda, Nutman, Amir, Adler, Amos, Levi, Inbar, Daikos, George L., Skiada, Anna, Pavleas, Ioannis, Antoniadou, Anastasia, Kotsaki, Antigoni, Durante-Mangoni, Emanuele, Andini, Roberto, Iossa, Domenico, Bernardo, Mariano, Cavezza, Giusi, Bertolino, Lorenzo, Giuffre, Giuseppe, Giurazza, Roberto, Cuccurullo, Susanna, Galdo, Maria, Murino, Patrizia, Cristinziano, Adriano, Corcione, Antonio, Zampino, Rosa, Pafundi, Pia Clara, Mouton, Johan, Friberg, Lena, Kristoffersson, Anders, Theuretzbacher, Ursula, Lellouche, Jonathan, Temkin, Elizabeth, Daikos, George, Dishon-Benattar, Yael, Friberg, Lena E., and Mouton, Johan W.

Published
2020
Full Text
View/download PDF

14. Antimicrobial consumption and resistance in adult hospital inpatients in 53 countries: results of an internet-based global point prevalence survey

Author

Koraqi, Andi, Hoxha, Iris, Tafaj, Silva, Lacej, Denada, Hojman, Martin, Quiros, Rodolfo Ernesto, Ghazaryan, Lilit, Cairns, Kelly A, Cheng, Allen, Horne, Kylie C, Doukas, Fiona F, Gottlieb, Thomas, Alsalman, Jameela, Magerman, Koen, Marielle, Gounongbe YT, Ljubovic, Amela Dedeic, Coelho, André Afonso Machado, Gales, Ana Cristina, Keuleyan, Emma, Sabuda, Deana, Boswell, Jennifer Lee, Conly, John M, Rojas, Alvaro, Carvajal, Camila, Labarca, Jaime, Solano, Antonio, Valverde, Carlos Ramírez, Villalobos-Vindas, Juan M, Pristas, Irina, Plecko, Vanda, Paphitou, Niki, Shaqiri, Erjona, Rummukainen, Maija-Liisa, Pagava, Karaman, Korinteli, Irma, Brandt, Tobias, Messler, Sabine, Enimil, Anthony, Iosifidis, Elias, Roilides, Emmanuel, Sow, Mamadou Saliou, Sengupta, Sharmila, George, Joby V, Poojary, Aruna, Patil, Priyanka, Soltani, Jafar, Jafarpour, Zahra, Ameen, Hadi, Fitzgerald, David, Maor, Yasmin, Chowers, Michal, Temkin, Elizabeth, Esposito, Susanna, Arnoldo, Luca, Brusaferro, Silvio, Gu, Yoshiaki, El-Hajji, Feras Darwish, Kim, Nam Joong, Kambaralieva, Baktygul, Pavare, Jana, Zarakauska, Lelde, Usonis, Vytautas, Burokiene, Sigita, Ivaskeviciene, Inga, Mijovic, Gordana, Duborija-Kovacevic, Natasa, Bondesio, Kristen, Iregbu, Kenneth, Oduyebo, Oyinlola, Raka, Denis, Raka, Lul, Rachina, Svetlana, Enani, Mushira Abdulaziz, Al Shehri, Mohamed, Carevic, Biljana, Dragovac, Gorana, Obradovic, Dusanka, Stojadinovic, Aleksandra, Radulovic, Lili, Wu, Jia EN, Wei Teng Chung, Gladys, Chen, Hui Hiong, Tambyah, Paul Anantharajah, Lye, David, Tan, Sock Hoon, Ng, Tat Ming, Tay, Hui Lin, Ling, Moi Lin, Chlebicki, Maciej Piotr, Kwa, Andrea L, Lee, Winnie, Beović, Bojana, Dramowski, Angela, Finlayson, Heather, Taljaard, Jantjie, Ojeda-Burgos, Guillermo, Retamar, Pilar, Lucas, Johan, Pot, Wolter, Verduin, Cees, Kluytmans, Jan, Scott, Michael, Aldeyab, Mamoon A, McCullagh, Bernadette, Gormley, Cairine, Sharpe, David, Gilchrist, Mark, Whitney, Laura, Laundy, Matthew, Lockwood, Debbie, Drysdale, Simon B, Boudreaux, Jennifer, Septimus, Edward J, Greer, Nickie, Gawrys, Gerard, Rios, Edgar, May, Suzanne, Versporten, Ann, Zarb, Peter, Caniaux, Isabelle, Gros, Marie-Françoise, Drapier, Nico, Miller, Mark, Jarlier, Vincent, Nathwani, Dilip, and Goossens, Herman

Published
2018
Full Text
View/download PDF

15. Prevalence and Clinical Consequences of Colistin Heteroresistance and Evolution into Full Resistance in Carbapenem-Resistant Acinetobacter baumannii

Author

Kon, Hadas, primary, Hameir, Amichay, additional, Nutman, Amir, additional, Temkin, Elizabeth, additional, Keren Paz, Alona, additional, Lellouche, Jonathan, additional, Schwartz, David, additional, Weiss, David S., additional, Kaye, Keith S., additional, Daikos, George L., additional, Skiada, Anna, additional, Durante-Mangoni, Emanuele, additional, Dishon Benattar, Yael, additional, Yahav, Dafna, additional, Daitch, Vered, additional, Bernardo, Mariano, additional, Iossa, Domenico, additional, Friberg, Lena E., additional, Theuretzbacher, Ursula, additional, Leibovici, Leonard, additional, Dickstein, Yaakov, additional, Pollak, Dina, additional, Mendelsohn, Sigal, additional, Paul, Mical, additional, Carmeli, Yehuda, additional, Bitterman, Roni, additional, Zayyad, Hiba, additional, Koppel, Fidi, additional, Zak-Doron, Yael, additional, Altunin, Sergey, additional, Andria, Nizar, additional, Neuberger, Ami, additional, Stern, Anat, additional, Petersiel, Neta, additional, Raines, Marina, additional, Karban, Amir, additional, Eliakim-Raz, Noa, additional, Zusman, Oren, additional, Elbaz, Michal, additional, Atamna, Heyam, additional, Babich, Tanya, additional, Adler, Amos, additional, Levi, Inbar, additional, Pavleas, Ioannis, additional, Antoniadou, Anastasia, additional, Kotsaki, Antigoni, additional, Andini, Roberto, additional, Cavezza, Giusi, additional, Bertolino, Lorenzo, additional, Giuffre, Giuseppe, additional, Giurazza, Roberto, additional, Ruocco, Giuseppe, additional, Galdo, Maria, additional, Murino, Patrizia, additional, Cristinziano, Adriano, additional, Corcione, Antonio, additional, Zampino, Rosa, additional, Mouton, Johan, additional, and Friberg, Lena, additional

Published
2023
Full Text
View/download PDF

18. Prevalence and Clinical Consequences of Colistin Heteroresistance and Evolution into Full Resistance in Carbapenem-Resistant Acinetobacter baumannii

Author

Kon, Hadas, Hameir, Amichay, Nutman, Amir, Temkin, Elizabeth, Keren Paz, Alona, Lellouche, Jonathan, Schwartz, David, Weiss, David S., Kaye, Keith S., Daikos, George L., Skiada, Anna, Durante-Mangoni, Emanuele, Dishon Benattar, Yael, Yahav, Dafna, Daitch, Vered, Bernardo, Mariano, Iossa, Domenico, Friberg, Lena E., Theuretzbacher, Ursula, Leibovici, Leonard, Dickstein, Yaakov, Pollak, Dina, Mendelsohn, Sigal, Paul, Mical, Carmeli, Yehuda, Grp, AIDA Study, Kon, Hadas, Hameir, Amichay, Nutman, Amir, Temkin, Elizabeth, Keren Paz, Alona, Lellouche, Jonathan, Schwartz, David, Weiss, David S., Kaye, Keith S., Daikos, George L., Skiada, Anna, Durante-Mangoni, Emanuele, Dishon Benattar, Yael, Yahav, Dafna, Daitch, Vered, Bernardo, Mariano, Iossa, Domenico, Friberg, Lena E., Theuretzbacher, Ursula, Leibovici, Leonard, Dickstein, Yaakov, Pollak, Dina, Mendelsohn, Sigal, Paul, Mical, Carmeli, Yehuda, and Grp, AIDA Study

Abstract

Colistin heteroresistance (HR) refers to a bacterial population comprised of several subpopulations with different levels of resistance to colistin. In this study, we discuss the classic form of HR, in which a resistant subpopulation exists within a predominantly susceptible population. We investigated the prevalence of colistin HR and its evolution into full resistance among 173 clinical carbapenem-resistant Acinetobacter baumannii isolates and examined the effect of HR on clinical outcomes. To determine HR, we performed population analysis profiling. Our results showed a high prevalence of HR (67.1%). To examine evolution of HR strains into full resistance, the HR strains were grown in colistin-containing broth, transferred onto colistin-containing plates, and colonies on these plates were transferred into colistin-free broth. Many of the HR strains (80.2%) evolved into full resistance, 17.2% reverted to HR, and 2.6% were borderline. We used logistic regression to compare 14-day clinical failure and 14-day mortality between patients infected by HR versus susceptible non-HR carbapenem-resistant A. baumannii. In the subgroup of patients with bacteremia, HR was significantly associated with 14-day mortality.IMPORTANCE To our knowledge, this is the first large-scale study to report on HR in Gram-negative bacteria. We described the prevalence of colistin HR in a large sample of carbapenem-resistant A. baumannii isolates, the evolution of many colistin HR isolates to a resistant phenotype following colistin exposure and withdrawal, and the clinical consequences of colistin HR. We found a high prevalence of HR among clinical carbapenem-resistant A. baumannii isolates; most evolved into a resistant phenotype following colistin exposure and withdrawal. In patients treated with colistin, evolution of HR A. baumannii into full resistance could lead to higher rates of treatment failure and contribute to the reservoir of colistin-resistant pathogens in health care settings. To

Published
2023
Full Text
View/download PDF

26. Hospital-acquired bacterial infections in coronavirus disease 2019 (COVID-19) patients in Israel

Author

Schwaber, Mitchell J., Temkin, Elizabeth, Lobl, Rona, Schechner, Vered, Nutman, Amir, and Carmeli, Yehuda

Abstract

AbstractBackground:We sought to determine incidence of common hospital-acquired bacteria among coronavirus disease 2019 (COVID-19) patients in Israeli general hospitals during the first year of the pandemic.Methods:We analyzed routinely collected incidence data to determine hospital acquisition of the following sentinel bacteria: Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa, Acinetobacter baumannii, and Clostridioides difficile. We examined 3 acquisition measures: (1) sentinel bacteria, (2) sentinel bacteremia, and (3) antimicrobial-resistant sentinel bacteremia. The study period was March 1, 2020, through January 31, 2021.Results:Analysis of pooled data from the 26 hospitals surveyed revealed that rates were higher for all 3 acquisition measures among COVID-19 patients than they were among patients on general medical wards in 2019, but lower than those among patients in intensive care units in 2019. The incidence rate was highest during the first COVID-19 wave, despite a lower proportion of severe COVID-19 cases among total hospitalized during this wave. Wide variation in incidence was evident between hospitals.Conclusions:Hospitalized COVID-19 patients experienced nosocomial bacterial infection at rates higher than those of patients on pre-pandemic general medical wards, adding to the complexity of their care. Lower rates of nosocomial infection after the first wave, despite higher proportions of severely ill patients, suggest that healthcare worker practices, rather than patient-related factors, were responsible for most of these infections.

Published
2023
Full Text
View/download PDF

27. Hospital antibiotic prescribing patterns in adult patients according to the WHO Access, Watch and Reserve classification (AWaRe): results from a worldwide point prevalence survey in 69 countries

Author

Pauwels, Ines, Versporten, Ann, Drapier, Nico, Vlieghe, Erika, Goossens, Herman, Koraqi, Andi, Hoxha, Iris, Tafaj, Silva, Cornistein, Wanda, Quiros, Rodolfo, Hojman, Martin, Ghazaryan, Lilit, Horne, Kylie, Cairns, Kelly, Doukas, Fiona, Gottlieb, Thomas, Sermijn, Erica, Verhamme, Katia, Brands, Christiane, Van Herendael, Bruno, Filippin, Lorenzo, Vandewal, Wouter, Konopnicki, Deborah, Maillart, Evelyne, Teixeira Lopes, Liliana, Papin, Pauline, Smits, Ilse, Jansens, Hilde, Bartholomeus, Sofie, Van den Abeele, Anne-Marie, Steyaert, Sophia, Piette, Anne, Buyle, Franky, Cartuyvels, Reinoud, Jonckheere, Stijn, Wybo, Ingrid, Vanneste, Lorenz, Mathieu, Delphine, Firre, Eric, Westelinck, Veerle, Gadisseux, Philippe, Dugernier, Thierry, Bafort, Kristof, Gonissen, Viviane, Vanderper, Vanessa, Gabriels, Patrick, Weekers, Frank, Michel, Philippe, Van Liedekerke, Ann, Costers, Michiel, Catry, Boudewijn, Dedeic-Ljubovic, Amela, Gales, Ana C., Matos Porto, Ana Paula, Figueiredo Costa, Silvia, Keuleyan, Emma, Beidi, Apollinaire, Cissohko, Youssouph, Blakwe, Habsatou, Batchaya Basile, Ngassa, German, Greg J., Lutes, Sarah, Boswell, Jennifer, Mertz, Dominik, Nguyen, Tuyen, MacLaggan, Timothy, Landry, Daniel, Ang, Anita, Thirion, Daniel J.G., Frenette, Charles, Émond, Yannick, Roberts, Jacqueline, Chang, Sandra, Kosar, Justin, Valiquette, Louis, Dutrisac, Ginette, Afra, Kevin, McGeer, Allison, Carrier, Marie, Grant, Jennifer, Labarca, Jaime, Carvajal, Camila, Lin, HongYi, Wang, Qiang, Yang, Jing, Yang, Wenjie, Cortes, Jorge A., Villalobos-Vindas, Juan, Ramírez-Valverde, Carlos, Horvatic, Jasminka, Pristas, Irina, Paphitou, Niki, Rummukainen, Maija-Liisa, Froissart, Antoine, Vanhems, Philippe, Pagava, Karaman, Korinteli, Irma, Brandt, Tobias, Gaertner, Johannes, Enimil, Anthony, Roilides, Emmanuel, Hajdú, Edit, Sengupta, Sharmila, Singh, Sanjeev, Patil, Priyanka, Poojary, Aruna, Soltani, Jafar, Pouladfar, Gholamreza, Jafarpour, Zahra, Alinia, Cyrus, Ameen, Hadi, Fitzgerald, David, Paul, Mical, Maor, Yasmin, Strahilevitz, Jacob, Chowers, Michal, Temkin, Elizabeth, Luca, Arnoldo, Ishibashi, Noriomi, Gu, Yoshiaki, Darwish Elhajji, Feras, Karabukayeva, Aizhan, Raka, Denis, Kambaralieva, Baktygul, Zarakauska, Lelde, Zarb, Peter, Hernandez Chena, Blanca Estela, Gonzalez-Diaz, Esteban, Corona-Meléndez, JuanCarlos, Torres Erazo, Darwin Stalin, Loza-Jalil, Suria Elizabeth, Molina, Julio, Candelas, Jose Antonio, Mijovic, Gordana, Duborija-Kovacevic, Natasa, Jong, Eefje, Kluytmans, Jan, van Elzakker, Erika, Schweitzer, Valentijn, Davies, Nicola, Iregbu, Kenneth, Nwajiobi-Princewill, Philip, Nwafia, Ifeyinwa, Fasuyi, Temitayo, Aboderin, Aaron, Elikwu, Charles John, Fadeyi, Abayomi, Ola-Bello, Olafoyekemi, Oduyebo, Oyinlola, Adedosu, Akin Nelson, Ekuma, Agantem, Shaqiri, Erjona, Saleem, Zikria, De Los Reyes, Mari Rose, Tavares, Luis, Kim, Nam Joong, Rachina, Svetlana, Alharthi, Alwaleed R., Enani, Mushira, Faried, Osama, Mirghani, Mohamed, Carevic, Biljana, Radulovic, Lili, Dragovac, Gorana, Tan, Sock Hoon, Taljaard, Jantjie, Chibabhai, Vindana, Joiner, Jennifer, Caston, Juan Jose, Núñez-Núñez, María, Martínez-Marcos, Francisco Javier, Ojeda-Burgos, Guillermo, Menendez, Maria Dolores, Retamar, Pilar, Corzo, Juan E., Rattanaumpawan, Pinyo, Salou, Mounerou, Mnif, Basma, Oncul, Ahsen, Babigumira, Peter Ahabwe, Olweny, James, Marshall, Emily, McCorry, Ann, Aldeyab, Mamoon, Khanna, Priya, Gormley, Cairine, Maloney, Sara, Cooper, Mandelin, Blackburn, Laura, Gessner-Wharton, Mallory, Vu, Lam, Greer, Nickie, Gawrys, Gerard, Kronmann, Lisha, Rios, Edgar, Hudson, Melissa, Lindholm, David A., The Global-PPS network, Vriendenkring VUB, Clinical sciences, Microbiology and Infection Control, and Clinical Biology

Subjects
Adult, Microbiology (medical), Point prevalence survey, Latin Americans, education, Population, MEDLINE, World Health Organization, Essential medicines, Antibiotic prescribing, Anti-Infective Agents, Environmental health, Prevalence, Humans, AcademicSubjects/MED00740, Medicine, Pharmacology (medical), Medical prescription, Biology, Original Research, Pharmacology, education.field_of_study, Adult patients, business.industry, Pharmacology. Therapy, Hospitals, Anti-Bacterial Agents, Anti-Bacterial Agents/therapeutic use, AcademicSubjects/MED00290, Infectious Diseases, Human medicine, AcademicSubjects/MED00230, business
Abstract

ObjectivesThe WHO Access, Watch and Reserve (AWaRe) classification has been developed to support countries and hospitals in promoting rational use of antibiotics while improving access to these essential medicines. We aimed to describe patterns of worldwide antibiotic use according to the AWaRe classification in the adult inpatient population.MethodsThe Global Point Prevalence Survey on Antimicrobial Consumption and Resistance (Global-PPS) collects hospital antibiotic use data using a standardized PPS methodology. Global-PPS 2015, 2017 and 2018 data, collected by 664 hospitals in 69 countries, were categorized into AWaRe groups to calculate proportional AWaRe use, Access-to-Watch ratios and the most common indications for treatment with selected Watch antibiotics. Only prescriptions for systemic antibiotics on adult inpatient wards were analysed.ResultsRegional Access use ranged from 28.4% in West and Central Asia to 57.7% in Oceania, whereas Watch use was lowest in Oceania (41.3%) and highest in West and Central Asia (66.1%). Reserve use ranged from 0.03% in sub-Saharan Africa to 4.7% in Latin America. There were large differences in AWaRe prescribing at country level. Watch antibiotics were prescribed for a range of very different indications worldwide, both for therapeutic and prophylactic use.ConclusionsWe observed considerable variations in AWaRe prescribing and high use of Watch antibiotics, particularly in lower- and upper-middle-income countries, followed by high-income countries. The WHO AWaRe classification has an instrumental role to play in local and national stewardship activities to assess prescribing patterns and to inform and evaluate stewardship activities.

Published
2021

30. Large-scale WGS of carbapenem-resistant Acinetobacter baumannii isolates reveals patterns of dissemination of ST clades associated with antibiotic resistance

Author

Frenk, Sammy, Temkin, Elizabeth, Lurie-Weinberger, Mor N., Keren-Paz, Alona, Rov, Reut, Rakovitsky, Nadya, Wullfhart, Liat, Nutman, Amir, Daikos, George L., Skiada, Anna, Durante-Mangoni, Emanuele, Benattar, Yael Dishon, Bitterman, Roni, Yahav, Dafna, Daitch, Vered, Bernardo, Mariano, Iossa, Domenico, Zusman, Oren, Friberg, Lena, Mouton, Johan W., Theuretzbacher, Ursula, Leibovici, Leonard, Geffen, Yuval, Gershon, Rina, Paul, Mical, Carmeli, Yehuda, Frenk, Sammy, Temkin, Elizabeth, Lurie-Weinberger, Mor N., Keren-Paz, Alona, Rov, Reut, Rakovitsky, Nadya, Wullfhart, Liat, Nutman, Amir, Daikos, George L., Skiada, Anna, Durante-Mangoni, Emanuele, Benattar, Yael Dishon, Bitterman, Roni, Yahav, Dafna, Daitch, Vered, Bernardo, Mariano, Iossa, Domenico, Zusman, Oren, Friberg, Lena, Mouton, Johan W., Theuretzbacher, Ursula, Leibovici, Leonard, Geffen, Yuval, Gershon, Rina, Paul, Mical, and Carmeli, Yehuda

Abstract

Objectives: To describe the population genetics and antibiotic resistance gene distribution of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates causing infections in three Mediterranean countries. Methods: Isolates were collected during the 2013-17 AIDA clinical trial in six hospitals in Israel, Greece and Italy. WGS, bioinformatic characterization and antibiotic resistance profiling were performed. Results In the 247 CRAB isolates characterized in this study, ST distribution varied by country: 29/31 (93.5%) Greek isolates, 34/41 (82.9%) Italian isolates and 70/175 (40.0%) Israeli isolates belonged to ST2. The identified ST2 isolates included eight distinct clades: 2C, 2D and 2H were significantly more common in Italy, while 2F was unique to Greece. The uncommon ST3 was not present among Greek isolates and constituted only 5/41 (12%) Italian isolates. On the other hand, it was much more common among Israeli isolates: 78/175 (44.6%) belonged to ST3. The vast majority of isolates, 240/247 (97.2%), were found to harbour acquired carbapenemases, primarily bla(OXA-23.) The chromosomal oxaAb (bla(OXA-51-like)) and ampC genes characteristic of this organism were also ubiquitous. Most (96.4%) ST3 isolates carried a broad-host-range plasmid IncP1 alpha. Conclusions The geographical differences in CRAB populations support the theory that clonal spread of CRAB leads to endemicity in hospitals and regions. The close association between antibiotic resistance genes and clades, and between plasmids and STs, suggest that de novo creation of MDR A. baumannii is rare. The clustering of antibiotic resistance genes and plasmids that is unique to each clade/ST, and nearly uniform within clades/STs, suggests that horizontal transmission is rare but crucial to the clade's/ST's success.

Published
2022
Full Text
View/download PDF

31. In vivo fitness of carbapenem-resistant Acinetobacter baumannii strains in murine infection is associated with treatment failure in human infections

Author

Nutman, Amir, Temkin, Elizabeth, Lellouche, Jonathan, Rakovitsky, Nadya, Hameir, Amichay, Daikos, George, Durante-Mangoni, Emanuele, Pavleas, Ioannis, Dishon, Yael, Petersiel, Neta, Yahav, Dafna, Eliakim, Noa, Bernardo, Mariano, Iossa, Domenico, Friberg, Lena, Theuretzbacher, Ursula, Leibovici, Leonard, Paul, Mical, Carmeli, Yehuda, Nutman, Amir, Temkin, Elizabeth, Lellouche, Jonathan, Rakovitsky, Nadya, Hameir, Amichay, Daikos, George, Durante-Mangoni, Emanuele, Pavleas, Ioannis, Dishon, Yael, Petersiel, Neta, Yahav, Dafna, Eliakim, Noa, Bernardo, Mariano, Iossa, Domenico, Friberg, Lena, Theuretzbacher, Ursula, Leibovici, Leonard, Paul, Mical, and Carmeli, Yehuda

Abstract

Objectives: Mortality among patients with carbapenem-resistant Acinetobacter baumannii (CRAB) infections varies between studies. We examined whether in vivo fitness of CRAB strains is associated with clinical outcomes in patients with CRAB infections. Methods: Isolates were collected from patients enrolled in the AIDA trial with hospital-acquired pneumonia, bloodstream infections and/or urinary tract infections caused by CRAB. The primary outcome was 14-day clinical failure, defined as failure to meet all criteria: alive; haemodynamically stable; improved or stable Sequential Organ Failure Assessment (SOFA) score; improved or stable oxygenation; and microbiological cure of bacteraemia. The secondary outcome was 14-day mortality. We tested in vivo growth using a neutropenic murine thigh infection model. Fitness was defined based on the CFU count 24 hours after injection of an inoculum of 105 CFU. We used mixed-effects logistic regression to test the association between fitness and the two outcomes. Results: The sample included 266 patients; 215 (80.8%) experienced clinical failure. CRAB fitness ranged from 5.23 to 10.08 log CFU/g. The odds of clinical failure increased by 62% for every 1-log CFU/g increase in fitness (OR 1.62, 95% CI 1.04-2.52). After adjusting for age, Charlson score, SOFA score and acquisition in the intensive care unit, fitness remained significant (adjusted OR 1.63, 95% CI 1.03-2.59). CRAB fitness had a similar effect on 14-day mortailty, although the association was not statistically significant (OR 1.56, 95% CI 0.95-2.57). It became significant after adjusting for age, Charlson score, SOFA score and recent surgery (adjusted OR 1.88, 95% CI 1.09-3.25). Conclusions: In vivo CRAB fitness was associated with clinical failure in patients with CRAB infection.

Published
2022
Full Text
View/download PDF

34. In vivo fitness of carbapenem-resistant Acinetobacter baumannii strains in murine infection is associated with treatment failure in human infections

Author

Nutman, Amir Temkin, Elizabeth Lellouche, Jonathan and Rakovitsky, Nadya Hameir, Amichay Daikos, George and Durante-Mangoni, Emanuele Pavleas, Ioannis Dishon, Yael and Petersiel, Neta Yahav, Dafna Eliakim, Noa Bernardo, Mariano and Iossa, Domenico Friberg, Lena E. Theuretzbacher, Ursula and Leibovici, Leonard Paul, Mical Carmeli, Yehuda AIDA Study Grp

Abstract

Objectives: Mortality among patients with carbapenem-resistant Acinetobacter baumannii (CRAB) infections varies between studies. We examined whether in vivo fitness of CRAB strains is associated with clinical outcomes in patients with CRAB infections. Methods: Isolates were collected from patients enrolled in the AIDA trial with hospital-acquired pneumonia, bloodstream infections and/or urinary tract infections caused by CRAB. The primary outcome was 14-day clinical failure, defined as failure to meet all criteria: alive; haemodynamically stable; improved or stable Sequential Organ Failure Assessment (SOFA) score; improved or stable oxygenation; and microbiological cure of bacteraemia. The secondary outcome was 14-day mortality. We tested in vivo growth using a neutropenic murine thigh infection model. Fitness was defined based on the CFU count 24 hours after injection of an inoculum of 105 CFU. We used mixed-effects logistic regression to test the association between fitness and the two outcomes. Results: The sample included 266 patients; 215 (80.8%) experienced clinical failure. CRAB fitness ranged from 5.23 to 10.08 log CFU/g. The odds of clinical failure increased by 62% for every 1-log CFU/g increase in fitness (OR 1.62, 95% CI 1.04-2.52). After adjusting for age, Charlson score, SOFA score and acquisition in the intensive care unit, fitness remained significant (adjusted OR 1.63, 95% CI 1.03-2.59). CRAB fitness had a similar effect on 14-day mortailty, although the association was not statistically significant (OR 1.56, 95% CI 0.95-2.57). It became significant after adjusting for age, Charlson score, SOFA score and recent surgery (adjusted OR 1.88, 95% CI 1.09-3.25). Conclusions: In vivo CRAB fitness was associated with clinical failure in patients with CRAB infection. Amir Nutman, Clin Microbiol Infect 2022;28:73 (c) 2021 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

Published
2022

35. Large-scale WGS of carbapenem-resistant Acinetobacter baumannii isolates reveals patterns of dissemination of ST clades associated with antibiotic resistance

Author

Frenk, Sammy Temkin, Elizabeth Lurie-Weinberger, Mor N. Keren-Paz, Alona Rov, Reut Rakovitsky, Nadya Wullfhart, Liat Nutman, Amir Daikos, George L. Skiada, Anna Durante-Mangoni, Emanuele Dishon Benattar, Yael Bitterman, Roni Yahav, Dafna Daitch, Vered Bernardo, Mariano Iossa, Domenico Zusman, Oren Friberg, Lena E. Mouton, Johan W. Theuretzbacher, Ursula Leibovici, Leonard Geffen, Yuval Gershon, Rina Paul, Mical Carmeli, Yehuda

Abstract

OBJECTIVES: To describe the population genetics and antibiotic resistance gene distribution of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates causing infections in three Mediterranean countries. METHODS: Isolates were collected during the 2013-17 AIDA clinical trial in six hospitals in Israel, Greece and Italy. WGS, bioinformatic characterization and antibiotic resistance profiling were performed. RESULTS: In the 247 CRAB isolates characterized in this study, ST distribution varied by country: 29/31 (93.5%) Greek isolates, 34/41 (82.9%) Italian isolates and 70/175 (40.0%) Israeli isolates belonged to ST2. The identified ST2 isolates included eight distinct clades: 2C, 2D and 2H were significantly more common in Italy, while 2F was unique to Greece. The uncommon ST3 was not present among Greek isolates and constituted only 5/41 (12%) Italian isolates. On the other hand, it was much more common among Israeli isolates: 78/175 (44.6%) belonged to ST3. The vast majority of isolates, 240/247 (97.2%), were found to harbour acquired carbapenemases, primarily blaOXA-23. The chromosomal oxaAb (blaOXA-51-like) and ampC genes characteristic of this organism were also ubiquitous. Most (96.4%) ST3 isolates carried a broad-host-range plasmid IncP1α. CONCLUSIONS: The geographical differences in CRAB populations support the theory that clonal spread of CRAB leads to endemicity in hospitals and regions. The close association between antibiotic resistance genes and clades, and between plasmids and STs, suggest that de novo creation of MDR A. baumannii is rare. The clustering of antibiotic resistance genes and plasmids that is unique to each clade/ST, and nearly uniform within clades/STs, suggests that horizontal transmission is rare but crucial to the clade’s/ST’s success.

Published
2022

36. Large-scale WGS of carbapenem-resistant Acinetobacter baumannii isolates reveals patterns of dissemination of ST clades associated with antibiotic resistance

Author

Frenk, Sammy, primary, Temkin, Elizabeth, additional, Lurie-Weinberger, Mor N., additional, Keren-Paz, Alona, additional, Rov, Reut, additional, Rakovitsky, Nadya, additional, Wullfhart, Liat, additional, Nutman, Amir, additional, Daikos, George L., additional, Skiada, Anna, additional, Durante-Mangoni, Emanuele, additional, Dishon Benattar, Yael, additional, Bitterman, Roni, additional, Yahav, Dafna, additional, Daitch, Vered, additional, Bernardo, Mariano, additional, Iossa, Domenico, additional, Zusman, Oren, additional, Friberg, Lena E., additional, Mouton, Johan W., additional, Theuretzbacher, Ursula, additional, Leibovici, Leonard, additional, Geffen, Yuval, additional, Gershon, Rina, additional, Paul, Mical, additional, and Carmeli, Yehuda, additional

Published
2022
Full Text
View/download PDF

37. In vivo fitness of carbapenem-resistant Acinetobacter baumannii strains in murine infection is associated with treatment failure in human infections

Author

Nutman, Amir, primary, Temkin, Elizabeth, additional, Lellouche, Jonathan, additional, Rakovitsky, Nadya, additional, Hameir, Amichay, additional, Daikos, George, additional, Durante-Mangoni, Emanuele, additional, Pavleas, Ioannis, additional, Dishon, Yael, additional, Petersiel, Neta, additional, Yahav, Dafna, additional, Eliakim, Noa, additional, Bernardo, Mariano, additional, Iossa, Domenico, additional, Friberg, Lena E., additional, Theuretzbacher, Ursula, additional, Leibovici, Leonard, additional, Paul, Mical, additional, Carmeli, Yehuda, additional, Benattar, Yael Dishon, additional, Dickstein, Yaakov, additional, Bitterman, Roni, additional, Zayyad, Hiba, additional, Koppel, Fidi, additional, Zak-Doron, Yael, additional, Altunin, Sergey, additional, Andria, Nizar, additional, Neuberger, Ami, additional, Stern, Anat, additional, Raines, Marina, additional, Karban, Amir, additional, Eliakim-Raz, Noa, additional, Zusman, Oren, additional, Elbaz, Michal, additional, Atamna, Heyam, additional, Daitch, Vered, additional, Babich, Tanya, additional, Nutman, Amir, additional, Adler, Amos, additional, Levi, Inbar, additional, Daikos, George L., additional, Skiada, Anna, additional, Antoniadou, Anastasia, additional, Kotsaki, Antigoni, additional, Andini, Roberto, additional, Cavezza, Giusi, additional, Bertolino, Lorenzo, additional, Giuffre, Giuseppe, additional, Giurazza, Roberto, additional, Ruocco, Giuseppe, additional, Galdo, Maria, additional, Murino, Patrizia, additional, Cristinziano, Adriano, additional, Corcione, Antonio, additional, Zampino, Rosa, additional, Mouton, Johan, additional, and Friberg, Lena, additional

Published
2022
Full Text
View/download PDF

39. Extremely low prevalence of asymptomatic COVID-19 among healthcare workers caring for COVID-19 patients in Israeli hospitals: a cross-sectional study

Author

Temkin, Elizabeth, primary, Temkin, Elizabeth, additional, Schwaber, Mitchell J., additional, Solter, Ester, additional, Vaturi, Azza, additional, Hen, Dafna, additional, Lugassy, Carmela Gweta, additional, Teitler, Nava, additional, Nutman, Amir, additional, Rubinovitch, Bina, additional, Ben-Zvi, Haim, additional, Shbita, Rana, additional, Wolf, Natalia Halel, additional, Benenson, Shmuel, additional, Gross, Ilana, additional, Slama, Carole, additional, Ottolenghi, Miriam, additional, Gottesman, Tamar, additional, Lev, Shaul, additional, Beilin, Viktoria, additional, Berkov, Evgeny, additional, Cohen, Regev, additional, Fried, Talya Finn, additional, Afraimov, Marina, additional, Shitrit, Pnina, additional, Egozi, Noga Vered, additional, Yassin, Alia, additional, Laderman, Hani, additional, Friedman, Haya, additional, Wiener-Well, Yonit, additional, Ben-Dor, Michal Itach, additional, Ben-David, Debby, additional, Harar, Orna Schwartz, additional, Yakobov, Malka, additional, Yashaev, Shoshana, additional, Marchaim, Dror, additional, Estrin, Inna, additional, Bibliv, Alexandra, additional, Saadon, Hodaya, additional, Ben Yossef, Galit, additional, and Carmeli, Yehuda, additional

Published
2021
Full Text
View/download PDF

41. Carbapenem-resistant Acinetobacter baumanniiload in patients and their environment: the importance of detecting carriers

Author

Schechner, Vered, Lerner, Anat Or, Temkin, Elizabeth, and Carmeli, Yehuda

Abstract

AbstractThe environment surrounding 30 of 31 carriers of carbapenem-resistant Acinetobacter baumannii(CRAB) was contaminated by CRAB. The environmental CRAB loads were similar whether carriers were identified only by surveillance cultures (nonclinical carriers) or also had positive clinical cultures. Screening to detect and isolate nonclinical CRAB carriers may be important to prevent CRAB transmission.

Published
2023
Full Text
View/download PDF

42. Increased Capsule Thickness and Hypermotility Are Traits of Carbapenem-Resistant Acinetobacter baumannii ST3 Strains Causing Fulminant Infection

Author

Rakovitsky, Nadya, primary, Lellouche, Jonathan, additional, Ben David, Debby, additional, Frenk, Sammy, additional, Elmalih, Polet, additional, Weber, Gabriel, additional, Kon, Hadas, additional, Schwartz, David, additional, Wolfhart, Liat, additional, Temkin, Elizabeth, additional, and Carmeli, Yehuda, additional

Published
2021
Full Text
View/download PDF

46. Contraceptive equity: the birth control center of the international workers order

Author

Temkin, Elizabeth

Subjects
Health maintenance organizations -- Services, Birth control -- Finance, Company financing, Government, Health care industry
Abstract

The Equity in Prescription Insurance and Contraceptive Coverage Act, introduced in Congress in 1997 and still unpassed, seeks to redress health insurers' failure to pay for birth control as they pay for other prescription drugs, most paradoxically Viagra. In 1936 the International Workers Order (IWO), a fraternal society, became the first insurer to include contraception in its benefits package. A forerunner in the movement for prepaid medical care, the IWO offered its members primary care and contraceptive services for annual flat fees. Founded at a time when the legal status of contraception was in flux, the IWO's Birth Control Center was the only such clinic to operate on an insurance system. Recent state laws and judicial actions have revived the IWO's groundbreaking view of contraception as a basic preventive service deserving of insurance coverage. (Am J Public Health. 2007; 97:1749-1757. doi:l0.2105/AJPH.2006.098145)

Published
2007

47. Colistin plus meropenem for carbapenem-resistant Gram-negative infections : in vitro synergism is not associated with better clinical outcomes

Author

Nutman, Amir, Lellouche, Jonathan, Temkin, Elizabeth, Daikos, George, Skiada, Anna, Durante-Mangoni, Emanuele, Dishon-Benattar, Yael, Bitterman, Roni, Yahav, Dafna, Daitch, Vered, Bernardo, Mariano, Iossa, Domenico, Zusman, Oren, Friberg, Lena, Mouton, Johan W., Theuretzbacher, Ursula, Leibovici, Leonard, Paul, Mical, Carmeli, Yehuda, Nutman, Amir, Lellouche, Jonathan, Temkin, Elizabeth, Daikos, George, Skiada, Anna, Durante-Mangoni, Emanuele, Dishon-Benattar, Yael, Bitterman, Roni, Yahav, Dafna, Daitch, Vered, Bernardo, Mariano, Iossa, Domenico, Zusman, Oren, Friberg, Lena, Mouton, Johan W., Theuretzbacher, Ursula, Leibovici, Leonard, Paul, Mical, and Carmeli, Yehuda

Abstract

Objectives In vitro models showing synergism between polymyxins and carbapenems support combination treatment for carbapenem-resistant Gram-negative (CRGN) infections. We tested the association between the presence of in vitro synergism and clinical outcomes in patients treated with colistin plus meropenem. Methods This was a secondary analysis of AIDA, a randomized controlled trial comparing colistin with colistin–meropenem for severe CRGN infections. We tested in vitro synergism using a checkerboard assay. Based on the fractional inhibitory concentration (ΣFIC) index for each colistin–meropenem combination, we categorized results as synergistic, antagonistic or additive/indifferent. The primary outcome was clinical failure at 14 days. Secondary outcomes were 14- and 28-day mortality and microbiological failure. Results The sample included 171 patients with infections caused by carbapenem-resistant Acinetobacter baumannii (n = 131), Enterobacteriaceae (n = 37) and Pseudomonas aeuruginosa (n = 3). In vitro testing showed synergism for 73 isolates, antagonism for 20 and additivism/indifference for 78. In patients who received any colistin plus meropenem, clinical failure at 14 days was 59/78 (75.6%) in the additivism/indifference group (reference category), 54/73 (74.0%) in the synergism group (adjusted odds ratio (aOR) 0.76, 95% CI 0.31–1.83), and 11/20 (55%) in the antagonism group (aOR 0.77, 95% CI 0.22–2.73). There was no significant difference between groups for any secondary outcome. Comparing the synergism group to patients treated with colistin monotherapy, synergism was not protective against 14-day clinical failure (aOR 0.52, 95% CI 0.26–1.04) or 14-day mortality (aOR1.09, 95% CI 0.60–1.96). Discussion In vitro synergism between colistin and meropenem via checkerboard method did not translate into clinical benefit.

Published
2020
Full Text
View/download PDF

49. A multi-institutional outbreak of New Delhi metallo-β-lactamase–producing Escherichia coli with subsequent acquisition of the Klebsiella pneumoniae carbapenemase gene

Author

Solter, Ester, primary, Kwong, Jason C., additional, Walton, Aaron, additional, Sherry, Norelle, additional, Howden, Benjamin P., additional, Grayson, M. Lindsay, additional, Schwartz, David, additional, Temkin, Elizabeth, additional, Frenk, Sammy, additional, Carmeli, Yehuda, additional, and Schwaber, Mitchell J., additional

Published
2020
Full Text
View/download PDF

50. Analysis of four carbapenem-resistant Acinetobacter baumanniioutbreaks using Fourier-transform infrared spectroscopy

Author

Kon, Hadas, Temkin, Elizabeth, Elmalih, Polet, Keren-Paz, Alona, Ben-David, Debby, Najjar-Debbiny, Ronza, Gottesman, Tamar, and Carmeli, Yehuda

Abstract

AbstractWe used Fourier-transform infrared (FTIR) spectroscopy to analyze 4 carbapenem-resistant Acinetobacter baumanniioutbreaks. FTIR distinguished between isolates from different hospitals and uncovered the relatedness between isolates from acute-care hospitals and a post–acute-care hospital. Using higher cutoffs reveals more distant relationships and lower cutoffs support analyses of recent events.

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results