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A bacterial strain designated AMTAE16 was isolated from a root of wheat in Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate in the genus Paenibacilluswith its closest relative being Paenibacillus daejeonensis AP-20 with 99.0 % 16S rRNA gene sequence similarity. DNA–DNA hybridization studies showed a mean of 30 % DNADNA relatedness between strain AMTAE16 and the type strain of P. daejeonensis. The isolate was a Gram-stainvariable, motile and sporulating rod. Catalase and oxidase activities were positive. Gelatin and starch were hydrolysed but not casein. Growth was supported by many carbohydrates and organic acids as carbon source. MK-7 was the only menaquinone detected and anteiso-C, C and iso-C were the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, four unidentified phospholipids and two unidentified lipids. meso- Diaminopimelic acid was detected in the peptidoglycan. The DNA G+C content was 55.4 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain AMTAE16represents a novel species of the genus Paenibacillus, for which the name Paenibacillus hispanicus sp. nov. is proposed. The type strain is AMTAE16(=LMG 29501=CECT 9124).

A bacterial strain named BSTT44T was isolated in the course of a study of endophytic bacteria occurring in stems and roots of potato growing in a soil from Salamanca, Spain. The 16S rRNA gene sequence had 99.7% identity with respect to that of its closest relative, Pseudomonas psychrophila E-3T, and the next most closely related type strains were those of Pseudomonas fragi, with 99.6% similarity, Pseudomonas deceptionensis, with 99.2% similarity, and Pseudomonas lundensis, with 99.0% similarity; these results indicate that BSTT44T should be classified within the genus Pseudomonas. Analysis of the housekeeping genes rpoB, rpoD and gyrB confirmed its phylogenetic affiliation and showed identities lower than 92% in all cases with respect to the above-mentioned closest relatives. Cells of the strain bore one polar–subpolar flagellum. The respiratory quinone was Q-9.The major fatty acids were C16:0, C18:1v7c and summed feature 3 (C16:1v7c and/or C16:1v6c). The strain was oxidase-, catalase- and urease-positive and the arginine dihydrolase system was present, but tests for nitrate reduction, b-galactosidase production and aesculin hydrolysis were negative. It could grow at 35 8C and at pH 5–9.The DNA G+C content was 60.2 mol%. DNA–DNA hybridization results showed less than 48% relatedness with respect to the type strains of the four most closely related species. Therefore, the combined results of genotypic, phenotypic and chemotaxonomic analyses support the classification of strain BSTT44 into a novel species of the genus Pseudomonas, for which the name Pseudomonas endophytica sp. nov. is proposed. The type strain is BSTT44T (5LMG 28456T5CECT 8691T).

A bacterial strain designated A4STR04T was isolated from the inner root tissue of potatoes in Spain. Phylogenetic analysis based on the 16S rRNA gene sequence placed the isolate into the genus Fontibacillus, being most closely related to Fontibacillus panacisegetis KCTC 13564T with 99 % Identity. The isolate was observed to form Grampositive, motile and sporulating rods. The catalase test was found to be negative and oxidase positive. Nitrate was found to be reduced to nitrite. b-Galactosidase and caseinase were observed to be produced but the production of gelatinase, urease, arginine dehydrolase, ornithine and lysine decarboxylase was negative. Aesculin hydrolysis was found to be positive and acetoin production was negative. Growth was found to be supported by many carbohydrates and organic acids as carbon source. MK-7 was the only menaquinone detected and the major fatty acid (61.5 %) was identified as anteiso-C15:0, as occurs in the other species of genus Fontibacillus. The strain A4STR04T was found to display a complex lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a glycolipid, two phospholipids, a lipid and two aminophospholipids. Mesodiaminopimelic acid was detected in the peptidoglycan. The G?C content was determined to be 50.5 mol% (Tm). Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain A4STR04T (=LMG 28458 T = CECT 8693T) should be classified as representing a novel species of genus Fontibacillus, for hich the name Fontibacillus solani sp. nov. is proposed.

La alfalfa (Medicago sativa L.) es uno de los cultivos forrajeros más importante en todo el mundo, siendo la leguminosa forrajera más importante en el sur de Europa, dada su condición de cultivo plurianial, con una duración en el campo de 3-6 años, e incluso hasta 10 años en algunos casos, y al alto contenido en proteínas y energía que la convierte en un excelente alimento para el ganado.

La patata (Solanum tuberosum L.) es el primer cultivo mundial no perteneciente al grupo de los cereales, siendo clave en primer lugar para la alimentación humana, donde se consume bajo múltiples modalidades, y es asimismo una esencial materia prima utilizada en numerosas aplicaciones industriales, en especial por su contenido en almidón (López Córcoles y López Fuster, 2010). La planta de patata pertenece a la familia de las solanáceas, es originaria de la Cordillera Andina, en América del Sur, aunque fue introducida en Europa por los españoles entre 1530 y 1570, desarrollándose su cultivo en nuestro continente a partir del siglo XVIII.

Estrategia de recuperación residencial del frente marítimo de Varberg. Suecia

Las plantas absorben por sus raíces sus nutrientes que se encuentran en el suelo en forma de iones que se movilizan por difusión hasta las raíces. El fósforo (P) es un nutriente esencial para las plantas y, después del nitrógeno, es el elemento más importante que limita el crecimiento de las mismas. El P está presente en el suelo en forma orgánica y mineral y la cantidad total de P en la corteza terrestre se estima en unas 10^15 Tm.