1. Glucocorticoid receptor-dependent therapeutic efficacy of tauroursodeoxycholic acid in preclinical models of spinocerebellar ataxia type 3
- Author
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Duarte-Silva, Sara, Da Silva, Jorge Diogo, Monteiro-Fernandes, Daniela, Costa, Marta Daniela, Neves-Carvalho, Andreia, Raposo, Mafalda, Soares-Cunha, Carina, Correia, Joana S., Nogueira- Goncalves, Goncalo, Fernandes, Henrique S., Oliveira, Stephanie, Ferreira-Fernandes, Ana Rita, Rodrigues, Fernando, Pereira-Sousa, Joana, Vilasboas-Campos, Daniela, Guerreiro, Sara, Campos, Jonas, Meireles- Costa, Liliana, Rodrigues, Cecilia M.P., Cabantous, Stephanie, Sousa, Sergio F., Lima, Manuela, Teixeira-Castro, Andreia, and Maciel, Patricia
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Thermo Fisher Scientific Inc. ,Medical research -- Models -- Health aspects ,Medicine, Experimental -- Models -- Health aspects ,Nematoda -- Models -- Health aspects ,Scientific equipment and supplies industry -- Health aspects -- Models ,Corticosteroids -- Health aspects -- Models ,Spinocerebellar ataxia -- Models -- Health aspects ,Bile acids -- Models -- Health aspects ,Genes -- Models -- Health aspects ,Proteolysis -- Health aspects -- Models ,Nervous system diseases -- Health aspects -- Models ,Health care industry - Abstract
Spinocerebellar ataxia type 3 (SCA3) is an adult-onset neurodegenerative disease caused by a polyglutamine expansion in the ataxin-3 (ATXN3) gene. No effective treatment is available for this disorder, other than symptom-directed approaches. Bile acids have shown therapeutic efficacy in neurodegenerative disease models. Here, we pinpointed tauroursodeoxycholic acid (TUDCA) as an efficient therapeutic, improving the motor and neuropathological phenotype of SCA3 nematode and mouse models. Surprisingly, transcriptomic and functional in vivo data showed that TUDCA acts in neuronal tissue through the glucocorticoid receptor (GR), but independently of its canonical receptor, the farnesoid X receptor (FXR). TUDCA was predicted to bind to the GR, in a similar fashion to corticosteroid molecules. GR levels were decreased in disease-affected brain regions, likely due to increased protein degradation as a consequence of ATXN3 dysfunction being restored by TUDCA treatment. Analysis of a SCA3 clinical cohort showed intriguing correlations between the peripheral expression of GR and the predicted age at disease onset in presymptomatic subjects and FKBP5 expression with disease progression, suggesting this pathway as a potential source of biomarkers for future study. We have established a novel in vivo mechanism for the neuroprotective effects of TUDCA in SCA3 and propose this readily available drug for clinical trials in SCA3 patients., Introduction Neurodegenerative diseases are currently considered a growing epidemic in the aging population (1, 2), still lacking effective therapeutic options. For those disorders with an identified genetic cause, research has [...]
- Published
- 2024
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