1. C-6α- vs C-7α-Substituted Steroidal Aromatase Inhibitors: Which Is Better? Synthesis, Biochemical Evaluation, Docking Studies, and Structure-Activity Relationships
- Author
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Fernanda M.F. Roleira, Natércia Teixeira, Stefano Alcaro, Giosuè Costa, Elisiário J. Tavares da Silva, Cristina Amaral, Federica Moraca, Saul C. Costa, Carla L. Varela, Georgina Correia-da-Silva, Roleira, Fmf, Varela, C, Amaral, C, Costa, Sc, Correia-da-Silva, G, Moraca, F, Costa, G, Alcaro, S, Teixeira, Naa, and Tavares da Silva, Ej
- Subjects
Molecular model ,Stereochemistry ,medicine.drug_class ,Substituent ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Structure-Activity Relationship ,Non-competitive inhibition ,Drug Discovery ,medicine ,Humans ,Aromatase ,IC50 ,030304 developmental biology ,0303 health sciences ,Aromatase inhibitor ,biology ,Aromatase Inhibitors ,0104 chemical sciences ,Molecular Docking Simulation ,010404 medicinal & biomolecular chemistry ,chemistry ,Docking (molecular) ,biology.protein ,Molecular Medicine ,Steroids ,Methyl group - Abstract
C-6α and C-7α androstanes were studied to disclose which position among them is more convenient to functionalize to reach superior aromatase inhibition. In the first series, the study of C-6 versus C-7 methyl derivatives led to the very active compound 9 with IC50 of 0.06 μM and Ki = 0.025 μM (competitive inhibition). In the second series, the study of C-6 versus C-7 allyl derivatives led to the best aromatase inhibitor 13 of this work with IC50 of 0.055 μM and Ki = 0.0225 μM (irreversible inhibition). Beyond these findings, it was concluded that position C-6α is better to functionalize than C-7α, except when there is a C-4 substituent simultaneously. In addition, the methyl group was the best substituent, followed by the allyl group and next by the hydroxyl group. To rationalize the structure–activity relationship of the best inhibitor 13, molecular modeling studies were carried out.
- Published
- 2019