20 results on '"Tefit M"'
Search Results
2. Effects of hydroxyurea on malaria, parasite growth and adhesion in experimental models
- Author
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PINO, P., TAOUFIQ, Z., BRUN, M., TEFIT, M., FRANETICH, J. F., CICERON, L., KRISHNAMOORTHY, R., and MAZIER, D.
- Published
- 2006
3. [In Process Citation]
- Author
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Atanasova, R., Tefit, M., Guitard, J., Fairhead, C., Mazier, D., Hennequin, C., Institut de génétique et microbiologie [Orsay] (IGM), and Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2013
4. Mise au point d’un modèle murin de candidose disséminée hématogène à Candida glabrata à point de départ digestif
- Author
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Atanasova, R., primary, Tefit, M., additional, Guitard, J., additional, Fairhead, C., additional, Mazier, D., additional, and Hennequin, C., additional
- Published
- 2013
- Full Text
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5. Mise au point d’un modèle murin de candidose disséminée hématogène àCandida glabrata à point de départ digestif.
- Author
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Atanasova, R., Tefit, M., Guitard, J., Fairhead, C., Mazier, D., and Hennequin, C.
- Published
- 2013
- Full Text
- View/download PDF
6. Rapid and Specific Action of Methylene Blue against Plasmodium Transmission Stages.
- Author
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Saison N, Franetich JF, Pinilla YT, Hoffmann A, Boussougou-Sambe ST, Ngossanga B, Tefit M, Ashraf K, Amanzougaghene N, Tajeri S, Adegnika AA, Mazier D, and Borrmann S
- Abstract
Methylene blue (MB) is the oldest synthetic anti-infective. Its high potency against asexual and sexual stages of malaria parasites is well documented. This study aimed to investigate possible additional activities of MB in interfering with parasite transmission and determine target stages in Anopheles vectors and humans. MB's transmission-blocking activity was first evaluated by an ex vivo direct membrane feeding assay (DMFA) using Plasmodium falciparum field isolates. To investigate anti-mosquito stage activity, Plasmodium berghei -infected Anopheles stephensi mosquitoes were fed a second blood meal on mice that had been treated with methylene blue, 3, 6- and 15-days after the initial infectious blood meal. Anti-sporozoite and liver stage activities were evaluated in vitro and in vivo via sporozoite invasion and liver stage development assays, respectively. MB exhibited a robust inhibition of P. falciparum transmission in An. gambiae , even when added shortly before the DMFA but only a moderate effect against P. berghei oocyst development. Exposure of mature P. berghei and P. falciparum sporozoites to MB blocked hepatocyte invasion, yet P. berghei liver stage development was unaffected by MB. Our results indicate previously underappreciated rapid specific activities of methylene blue against Plasmodium transmission stages, preventing the establishment of both mosquito midgut and liver infections as the first essential steps in both hosts.
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- 2022
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7. The Phosphodiesterase Inhibitor Tadalafil Promotes Splenic Retention of Plasmodium falciparum Gametocytes in Humanized Mice.
- Author
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Barbieri D, Gomez L, Royer L, Dupuy F, Franetich JF, Tefit M, N'Dri ME, Mazier D, Silvie O, Moreno-Sabater A, and Lavazec C
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- Animals, Mice, Phosphodiesterase Inhibitors, Spleen, Tadalafil pharmacology, Malaria, Falciparum drug therapy, Malaria, Falciparum parasitology, Plasmodium falciparum
- Abstract
The persistence of erythrocytes infected with Plasmodium falciparum gametocytes in the bloodstream is closely related to the modulation of their mechanical properties. New drugs that increase the stiffness of infected erythrocytes may thus represent a novel approach to block malaria parasite transmission. The phosphodiesterase inhibitor tadalafil has been shown to impair the ability of infected erythrocytes to circulate in an in vitro model for splenic retention. Here, we used a humanized mouse model to address in vivo the effect of tadalafil on the circulation kinetics of mature gametocyte-infected erythrocytes. We show that stiff immature gametocyte-infected erythrocytes are retained in the spleen of humanized mice at rates comparable to that of the in vitro model. Accordingly, tadalafil-induced stiffening of mature gametocyte-infected erythrocytes impairs their circulation in the bloodstream and triggers their retention by the spleen. These in vivo results validate that tadalafil is a novel drug lead potentially capable of blocking malaria parasite transmission by targeting GIE mechanical properties., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Barbieri, Gomez, Royer, Dupuy, Franetich, Tefit, N’Dri, Mazier, Silvie, Moreno-Sabater and Lavazec.)
- Published
- 2022
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8. Potent Antiplasmodial Derivatives of Dextromethorphan Reveal the Ent -Morphinan Pharmacophore of Tazopsine-Type Alkaloids.
- Author
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Keita A, Franetich JF, Carraz M, Valentin L, Bordessoules M, Baron L, Bigeard P, Dupuy F, Geay V, Tefit M, Sarrasin V, Michel S, Lavazec C, Houzé S, Mazier D, Soulard V, Porée FH, and Duval R
- Abstract
The alkaloid tazopsine 1 was introduced in the late 2000s as a novel antiplasmodial hit compound active against Plasmodium falciparum hepatic stages, with the potential to develop prophylactic drugs based on this novel chemical scaffold. However, the structural determinants of tazopsine 1 bioactivity, together with the exact definition of the pharmacophore, remained elusive, impeding further development. We found that the antitussive drug dextromethorphan (DXM) 3 , although lacking the complex pattern of stereospecific functionalization of the natural hit, was harboring significant antiplasmodial activity in vitro despite suboptimal prophylactic activity in a murine model of malaria, precluding its direct repurposing against the disease. The targeted N -alkylation of nor -DXM 15 produced a small library of analogues with greatly improved activity over DXM 3 against P. falciparum asexual stages. Amongst these, N -2'-pyrrolylmethyl- nor -DXM 16i showed a 2- to 36-fold superior inhibitory potency compared to tazopsine 1 and DXM 3 against P. falciparum liver and blood stages, with respectively 760 ± 130 nM and 2.1 ± 0.4 μM IC
50 values, as well as liver/blood phase selectivity of 2.8. Furthermore, cpd. 16i showed a 5- to 8-fold increase in activity relative to DXM 3 against P. falciparum stages I-II and V gametocytes, with 18.5 μM and 13.2 μM IC50 values, respectively. Cpd. 16i can thus be considered a promising novel hit compound against malaria in the ent -morphinan series with putative pan cycle activity, paving the way for further therapeutic development (e.g., investigation of its prophylactic activity in vivo).- Published
- 2022
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9. A New Thienopyrimidinone Chemotype Shows Multistage Activity against Plasmodium falciparum, Including Artemisinin-Resistant Parasites.
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Bosson-Vanga H, Primas N, Franetich JF, Lavazec C, Gomez L, Ashraf K, Tefit M, Soulard V, Dereuddre-Bosquet N, Le Grand R, Donnette M, Mustière R, Amanzougaghene N, Tajeri S, Suzanne P, Malzert-Fréon A, Rault S, Vanelle P, Hutter S, Cohen A, Snounou G, Roques P, Azas N, Lagardère P, Lisowski V, Masurier N, Nguyen M, Paloque L, Benoit-Vical F, Verhaeghe P, and Mazier D
- Subjects
- Animals, Antimalarials chemistry, Artemisinins pharmacology, Cell Line, Tumor, Disease Models, Animal, Dogs, Drug Resistance physiology, Female, Hep G2 Cells, Humans, Liver parasitology, Macaca fascicularis, Madin Darby Canine Kidney Cells, Male, Mice, Mice, Inbred BALB C, Pyrimidinones chemistry, Antimalarials pharmacology, Malaria, Falciparum drug therapy, Plasmodium cynomolgi drug effects, Plasmodium falciparum drug effects, Plasmodium yoelii drug effects, Pyrimidinones pharmacology
- Abstract
Human malaria infection begins with a one-time asymptomatic liver stage followed by a cyclic symptomatic blood stage. For decades, the research for novel antimalarials focused on the high-throughput screening of molecules that only targeted the asexual blood stages. In a search for new effective compounds presenting a triple action against erythrocytic and liver stages in addition to the ability to block the transmission of the disease via the mosquito vector, 2-amino-thienopyrimidinone derivatives were synthesized and tested for their antimalarial activity. One molecule, named gamhepathiopine (denoted as "M1" herein), was active at submicromolar concentrations against both erythrocytic (50% effective concentration [EC
50 ] = 0.045 μM) and liver (EC50 = 0.45 μM) forms of Plasmodium falciparum. Furthermore, gamhepathiopine efficiently blocked the development of the sporogonic cycle in the mosquito vector by inhibiting the exflagellation step. Moreover, M1 was active against artemisinin-resistant forms (EC50 = 0.227 μM), especially at the quiescent stage. Nevertheless, in mice, M1 showed modest activity due to its rapid metabolization by P450 cytochromes into inactive derivatives, calling for the development of new parent compounds with improved metabolic stability and longer half-lives. These results highlight the thienopyrimidinone scaffold as a novel antiplasmodial chemotype of great interest to search for new drug candidates displaying multistage activity and an original mechanism of action with the potential to be used in combination therapies for malaria elimination in the context of artemisinin resistance. IMPORTANCE This work reports a new chemical structure that (i) displays activity against the human malaria parasite Plasmodium falciparum at 3 stages of the parasitic cycle (blood stage, hepatic stage, and sexual stages), (ii) remains active against parasites that are resistant to the first-line treatment recommended by the World Health Organization (WHO) for the treatment of severe malaria (artemisinins), and (iii) reduces transmission of the parasite to the mosquito vector in a mouse model. This new molecule family could open the way to the conception of novel antimalarial drugs with an original multistage mechanism of action to fight against Plasmodium drug resistance and block interhuman transmission of malaria.- Published
- 2021
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10. The Host Protein Aquaporin-9 is Required for Efficient Plasmodium falciparum Sporozoite Entry into Human Hepatocytes.
- Author
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Amanzougaghene N, Tajeri S, Yalaoui S, Lorthiois A, Soulard V, Gego A, Rametti A, Risco-Castillo V, Moreno A, Tefit M, van Gemert GJ, Sauerwein RW, Vaillant JC, Ravassard P, Pérignon JL, Froissard P, Mazier D, and Franetich JF
- Subjects
- Animals, Hepatocytes metabolism, Humans, Plasmodium falciparum, Protozoan Proteins genetics, Protozoan Proteins metabolism, Tetraspanin 28 metabolism, Aquaporins, Sporozoites metabolism
- Abstract
Hepatocyte invasion by Plasmodium sporozoites represents a promising target for innovative antimalarial therapy, but the molecular events mediating this process are still largely uncharacterized. We previously showed that Plasmodium falciparum sporozoite entry into hepatocytes strictly requires CD81. However, CD81-overexpressing human hepatoma cells remain refractory to P. falciparum infection, suggesting the existence of additional host factors necessary for sporozoite entry. Here, through differential transcriptomic analysis of human hepatocytes and hepatoma HepG2-CD81 cells, the transmembrane protein Aquaporin-9 ( AQP9 ) was found to be among the most downregulated genes in hepatoma cells. RNA silencing showed that sporozoite invasion of hepatocytes requires AQP9 expression. AQP9 overexpression in hepatocytes increased their permissiveness to P. falciparum . Moreover, chemical disruption with the AQP9 inhibitor phloretin markedly inhibited hepatocyte infection. Our findings identify AQP9 as a novel host factor required for P. falciparum sporozoite hepatocyte-entry and indicate that AQP9 could be a potential therapeutic target., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Amanzougaghene, Tajeri, Yalaoui, Lorthiois, Soulard, Gego, Rametti, Risco-Castillo, Moreno, Tefit, van Gemert, Sauerwein, Vaillant, Ravassard, Pérignon, Froissard, Mazier and Franetich.)
- Published
- 2021
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11. Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium.
- Author
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Bosson-Vanga H, Franetich JF, Soulard V, Sossau D, Tefit M, Kane B, Vaillant JC, Borrmann S, Müller O, Dereuddre-Bosquet N, Le Grand R, Silvie O, and Mazier D
- Subjects
- Animals, Anopheles parasitology, Erythrocytes parasitology, Female, Liver parasitology, Mice parasitology, Mice, Inbred BALB C, Antimalarials pharmacology, Methylene Blue pharmacology, Plasmodium cynomolgi drug effects, Plasmodium falciparum drug effects, Plasmodium yoelii drug effects
- Abstract
Background: In the context of malaria elimination/eradication, drugs that are effective against the different developmental stages of the parasite are highly desirable. The oldest synthetic anti-malarial drug, the thiazine dye methylene blue (MB), is known for its activity against Plasmodium blood stages, including gametocytes. The aim of the present study was to investigate a possible effect of MB against malaria parasite liver stages., Methods: MB activity was investigated using both in vitro and in vivo models. In vitro assays consisted of testing MB activity on Plasmodium falciparum, Plasmodium cynomolgi and Plasmodium yoelii parasites in human, simian or murine primary hepatocytes, respectively. MB in vivo activity was evaluated using intravital imaging in BALB/c mice infected with a transgenic bioluminescent P. yoelii parasite line. The transmission-blocking activity of MB was also addressed using mosquitoes fed on MB-treated mice., Results: MB shows no activity on Plasmodium liver stages, including hypnozoites, in vitro in primary hepatocytes. In BALB/c mice, MB has moderate effect on P. yoelii hepatic development but is highly effective against blood stage growth. MB is active against gametocytes and abrogates parasite transmission from mice to mosquitoes., Conclusion: While confirming activity of MB against both sexual and asexual blood stages, the results indicate that MB has only little activity on the development of the hepatic stages of malaria parasites.
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- 2018
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12. Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice.
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Soulard V, Bosson-Vanga H, Lorthiois A, Roucher C, Franetich JF, Zanghi G, Bordessoulles M, Tefit M, Thellier M, Morosan S, Le Naour G, Capron F, Suemizu H, Snounou G, Moreno-Sabater A, and Mazier D
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- Animals, Erythrocyte Transfusion, Female, Hepatocytes transplantation, Humans, Life Cycle Stages, Male, Mice, Transgenic, Sporozoites physiology, Disease Models, Animal, Liver parasitology, Malaria parasitology, Plasmodium falciparum growth & development, Plasmodium ovale growth & development
- Abstract
Experimental studies of Plasmodium parasites that infect humans are restricted by their host specificity. Humanized mice offer a means to overcome this and further provide the opportunity to observe the parasites in vivo. Here we improve on previous protocols to achieve efficient double engraftment of TK-NOG mice by human primary hepatocytes and red blood cells. Thus, we obtain the complete hepatic development of P. falciparum, the transition to the erythrocytic stages, their subsequent multiplication, and the appearance of mature gametocytes over an extended period of observation. Furthermore, using sporozoites derived from two P. ovale-infected patients, we show that human hepatocytes engrafted in TK-NOG mice sustain maturation of the liver stages, and the presence of late-developing schizonts indicate the eventual activation of quiescent parasites. Thus, TK-NOG mice are highly suited for in vivo observations on the Plasmodium species of humans.
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- 2015
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13. Critical role of IL-33 receptor ST2 in experimental cerebral malaria development.
- Author
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Palomo J, Reverchon F, Piotet J, Besnard AG, Couturier-Maillard A, Maillet I, Tefit M, Erard F, Mazier D, Ryffel B, and Quesniaux VF
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- Animals, Brain immunology, Brain parasitology, Brain pathology, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes pathology, CD8-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes pathology, Disease Models, Animal, Female, Inflammation etiology, Inflammation immunology, Inflammation pathology, Interleukin-1 Receptor-Like 1 Protein, Interleukin-33, Interleukins metabolism, Lymphocyte Activation, Malaria, Cerebral immunology, Malaria, Cerebral parasitology, Mice, Mice, Inbred C57BL, Mice, Knockout, Receptors, Interleukin deficiency, Receptors, Interleukin genetics, Malaria, Cerebral etiology, Plasmodium berghei immunology, Plasmodium berghei pathogenicity, Receptors, Interleukin metabolism
- Abstract
Cerebral malaria, a severe complication of Plasmodium falciparum infection, can be modeled in murine Plasmodium berghei ANKA (PbA) infection. PbA-induced experimental cerebral malaria (ECM) is CD8(+) T-cell mediated, and influenced by TH 1/TH 2 balance. Here, we show that IL-33 expression is increased in brain undergoing ECM and we address the role of the IL-33/ST2 pathway in ECM development. ST2-deficient mice were resistant to PbA-induced neuropathology. They survived >20 days with no ECM neurological sign and a preserved cerebral microcirculation, while WT mice succumbed within 10 days with ECM, brain vascular leakage, distinct microvascular pathology obstruction, and hemorrhages. Parasitemia and brain parasite load were similar in ST2-deficient and WT mice. Protection was accompanied by reduced brain sequestration of activated CD4(+) T cells and perforin(+) CD8(+) T cells. While IFN-γ and T-cell-attracting chemokines CXCL9 and CXCL10 were not affected in the absence of functional ST2 pathway, the local expression of ICAM-1, CXCR3, and LT-α, crucial for ECM development, was strongly reduced, and this may explain the diminished pathogenic T-cell recruitment and resistance to ECM. Therefore, IL-33 is induced in PbA sporozoite infection, and the pathogenic T-cell responses with local microvascular pathology are dependent on IL-33/ST2 signaling, identifying IL-33 as a new actor in ECM development., (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2015
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14. A mouse model for Candida glabrata hematogenous disseminated infection starting from the gut: evaluation of strains with different adhesion properties.
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Atanasova R, Angoulvant A, Tefit M, Gay F, Guitard J, Mazier D, Fairhead C, and Hennequin C
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- Animals, Caco-2 Cells, Cell Adhesion, Colony Count, Microbial, Disease Models, Animal, Epithelial Cells microbiology, Epithelial Cells pathology, Humans, Kinetics, Mice, Survival Analysis, Candida glabrata physiology, Candidiasis microbiology, Candidiasis pathology, Gastrointestinal Tract microbiology, Gastrointestinal Tract pathology
- Abstract
Adhesion to digestive mucosa is considered a crucial first step in the pathogenicity of invasive Candida infections. Candida glabrata disseminated infections predominantly start from the gut. A mouse model of disseminated infection starting from the gut was set up. Hematogenous dissemination was obtained after a low-protein diet followed by a regimen of cyclophosphamide-methotrexate and an oral inoculation of the yeasts via the drinking water. The liver was the first organ infected (day 7 post-infection), and lethality was 100% at day 21 post-infection. This new mouse model was used to compare the mortality rate and fungal burden in deep organs induced by 5 strains exhibiting different levels of adhesion to enterocyte Caco-2 cells, as determined in a test on 36 C. glabrata strains. In this model, no statistical difference of lethality was demonstrated between the strains, and fungal burden varied in kidneys and lungs but without correlation with the level of adhesion to enterocytes. Further studies using the model developed here allow analysis of the crossing of the digestive mucosa by yeasts, and help relate this to yet-poorly understood adhesion phenotypes.
- Published
- 2013
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15. Skin-draining lymph node priming is sufficient to induce sterile immunity against pre-erythrocytic malaria.
- Author
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Obeid M, Franetich JF, Lorthiois A, Gego A, Grüner AC, Tefit M, Boucheix C, Snounou G, and Mazier D
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- Animals, Erythrocytes parasitology, Female, Hepatocytes immunology, Hepatocytes parasitology, Humans, Immunity, Immunization, Malaria parasitology, Malaria Vaccines administration & dosage, Malaria Vaccines immunology, Mice, Mice, Inbred BALB C, Mice, Transgenic, Plasmodium yoelii growth & development, Skin parasitology, Sporozoites growth & development, Sporozoites immunology, Erythrocytes immunology, Liver immunology, Lymph Nodes immunology, Malaria immunology, Plasmodium yoelii immunology, Skin immunology
- Abstract
The Plasmodium-infected hepatocyte has been considered necessary to prime the immune responses leading to sterile protection after vaccination with attenuated sporozoites. However, it has recently been demonstrated that priming also occurs in the skin. We wished to establish if sterile protection could be obtained in the absence of priming by infected hepatocytes. To this end, we developed a subcutaneous (s.c.) immunization protocol where few, possibly none, of the immunizing irradiated Plasmodium yoelii sporozoites infect hepatocytes, and also used CD81-deficient mice non-permissive to productive hepatocyte infections. We then compared and contrasted the patterns of priming with those obtained by intradermal immunization, where priming occurs in the liver. Using sterile immunity as a primary read-out, we exploited an inhibitor of T-cell migration, transgenic mice with conditional depletion of dendritic cells and adoptive transfers of draining lymph node-derived T cells, to provide evidence that responses leading to sterile immunity can be primed in the skin-draining lymph nodes with little, if any, contribution from the infected hepatocyte., (Copyright © 2013 The Authors. Published by John Wiley and Sons, Ltd on behalf of EMBO.)
- Published
- 2013
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16. Rho kinase inhibition in severe malaria: thwarting parasite-induced collateral damage to endothelia.
- Author
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Taoufiq Z, Gay F, Balvanyos J, Ciceron L, Tefit M, Lechat P, and Mazier D
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- 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine analogs & derivatives, 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine pharmacology, Animals, Coculture Techniques, Endothelium, Vascular drug effects, Erythrocytes parasitology, Humans, Models, Biological, NF-kappa B metabolism, Protein Kinase Inhibitors pharmacology, Apoptosis, Endothelium, Vascular parasitology, Plasmodium falciparum immunology, rho-Associated Kinases antagonists & inhibitors, rho-Associated Kinases metabolism
- Abstract
Acute clinical manifestations of falciparum malaria, such as multiorgan failure and cerebral malaria, occur unpredictably and lead to coma and death within hours if left untreated. Despite the emergency administration of effective antimalarial drugs, 15%-20% of patients die. Other therapeutic approaches are therefore urgently needed. There is increasing evidence that endothelial changes play a key role in the pathogenesis of severe malaria. We therefore used coculture models to study interactions between infected erythrocytes and endothelium. We found that adhesion of Plasmodium falciparum to endothelial cells in vitro activated the Rho kinase signaling pathway, which is strongly involved in various vascular diseases. When added concomitantly with parasites, the Rho kinase inhibitor fasudil (HA-1077), a drug already in clinical use, decreased both NF-kappaB activation and endothelial cell apoptosis. Fasudil also helped to restore endothelial barrier integrity after P. falciparum adhesion. Rho kinase inhibition thus appears to be a promising adjunctive therapeutic approach to the management of severe human malaria.
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- 2008
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17. Transient supplementation of superoxide dismutase protects endothelial cells against Plasmodium falciparum-induced oxidative stress.
- Author
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Taoufiq Z, Pino P, Dugas N, Conti M, Tefit M, Mazier D, and Vouldoukis I
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- Animals, Apoptosis, Cattle, Cell Adhesion, Cell Separation, Endothelial Cells cytology, Endothelial Cells drug effects, Erythrocytes parasitology, Gene Products, tat genetics, Humans, Hydrogen Peroxide, Intercellular Adhesion Molecule-1 metabolism, Lung cytology, Nitric Oxide Synthase Type I metabolism, Superoxide Dismutase genetics, Superoxide Dismutase-1, Transfection, Endothelial Cells metabolism, Oxidative Stress, Plasmodium falciparum physiology, Superoxide Dismutase metabolism
- Abstract
The pathogenesis of cerebral malaria, a major complication of Plasmodium falciparum infection, relies on mechanisms such as cytokine production and cytoadherence of parasitized red blood cells (PRBCs) on microvascular endothelial cells. In this way parasites avoid spleen clearance by sequestration in post-capillary venules of various organs including the brain. Infected erythrocytes adhesion has also been shown to have molecular signaling consequences providing insight on how tissue homeostasis could be comprised by endothelium perturbation. Our previous work demonstrated that PRBCs adhesion to human lung endothelial cells (HLEC) induces caspases activation, oxidative stress and apoptosis. Cytoplasmic Cu/Zn superoxide dismutase (SOD1), which provides the first line of defense against oxidative stress within a cell, is now used as a treatment of numerous diseases including traumatic brain injury and ischemic stroke. In this report, we demonstrated that transient supplementation of SOD1 protects endothelial cells against P. falciparum induced oxidative stress and apoptosis. We also showed a significant decrease in PRBCs cytoadherence through a downregulation of ICAM-1 and an induction of iNOS. Protection of endothelium via antioxidant delivery may constitute a relevant strategy in cerebral malaria treatment.
- Published
- 2006
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18. Supplementation with gliadin-combined plant superoxide dismutase extract promotes antioxidant defences and protects against oxidative stress.
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Vouldoukis I, Conti M, Krauss P, Kamaté C, Blazquez S, Tefit M, Mazier D, Calenda A, and Dugas B
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- Animals, Antioxidants administration & dosage, Antioxidants therapeutic use, Apoptosis drug effects, Dietary Supplements, Gliadin administration & dosage, Gliadin therapeutic use, Hepatocytes drug effects, Mice, Mice, Inbred BALB C, Mitochondria, Liver drug effects, Oxidative Stress drug effects, Peroxynitrous Acid, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Superoxide Dismutase administration & dosage, Superoxide Dismutase pharmacology, Superoxide Dismutase therapeutic use, Antioxidants pharmacology, Cucumis melo, Gliadin pharmacology, Phytotherapy, Plant Extracts pharmacology, Triticum
- Abstract
The potential benefits to health of antioxidant enzymes supplied either through dietary intake or supplementation is still a matter of controversy. The development of dietary delivery systems using wheat gliadin biopolymers as a natural carrier represents a new alternative. Combination of antioxidant enzymes with this natural carrier not only delayed their degradation (i.e. the superoxide dismutase, SOD) during the gastrointestinal digestive process, but also promoted, in vivo, the cellular defences by strengthening the antioxidant status. The effects of supplementation for 28 days with a standardized melon SOD extract either combined (Glisodin) or not with gliadin, were evaluated on various oxidative-stress biomarkers. As already described there was no change either in superoxide dismutase, catalase or glutathione peroxidase activities in blood circulation or in the liver following non-protected SOD supplementation. However, animals supplemented with Glisodin showed a significant elevation in circulated antioxidant enzymes activities, correlated with an increased resistance of red blood cells to oxidative stress-induced hemolysis. In the presence of Sin-1, a chemical donor of peroxynitrites, mitochondria from hepatocytes regularly underwent membrane depolarization as the primary biological event of the apoptosis cascade. Hepatocytes isolated from animals supplemented with Glisodin presented a delayed depolarization response and an enhanced resistance to oxidative stress-induced apoptosis. It is concluded that supplementation with gliadin-combined standardized melon SOD extract (Glisodin) promoted the cellular antioxidant status and protected against oxidative stress-induced cell death., (2004 John Wiley & Sons, Ltd.)
- Published
- 2004
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19. Perforin-dependent brain-infiltrating cytotoxic CD8+ T lymphocytes mediate experimental cerebral malaria pathogenesis.
- Author
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Nitcheu J, Bonduelle O, Combadiere C, Tefit M, Seilhean D, Mazier D, and Combadiere B
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- Adoptive Transfer, Animals, Brain metabolism, Brain parasitology, Brain pathology, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes pathology, Cell Differentiation genetics, Cell Differentiation immunology, Cell Movement genetics, Female, Fluoresceins metabolism, Genetic Predisposition to Disease, Immunity, Innate genetics, Immunologic Memory genetics, Malaria, Cerebral genetics, Membrane Glycoproteins deficiency, Membrane Glycoproteins genetics, Mice, Mice, Inbred C57BL, Mice, Knockout, Perforin, Plasmodium berghei pathogenicity, Pore Forming Cytotoxic Proteins, Receptors, CCR5 biosynthesis, Receptors, CCR5 deficiency, Receptors, CCR5 genetics, Spleen chemistry, Spleen cytology, Spleen immunology, Spleen transplantation, Succinimides metabolism, T-Lymphocyte Subsets immunology, T-Lymphocyte Subsets metabolism, T-Lymphocyte Subsets pathology, T-Lymphocytes, Cytotoxic metabolism, Brain immunology, Cell Movement immunology, Malaria, Cerebral immunology, Malaria, Cerebral pathology, Membrane Glycoproteins physiology, Plasmodium berghei immunology, T-Lymphocytes, Cytotoxic immunology, T-Lymphocytes, Cytotoxic pathology
- Abstract
Experimental cerebral malaria (ECM) resulting from Plasmodium berghei ANKA infection involves T lymphocytes. However, the mechanisms of T cell-mediated pathogenesis remain unknown. We found that, in contrast to ECM-susceptible C57BL6 mice, perforin-deficient (PFP-KO) mice were resistant to ECM in the absence of brain lesions, whereas cytoadherence of parasitized erythrocytes and massive accumulation of activated/effector CD8 lymphocytes were observed in both groups of mice. ECM is induced in PFP-KO mice after adoptive transfer of cytotoxic CD8+ cells from infected C57BL6 mice, which were directed to the brain of PFP-KO mice. This specific recruitment might involve chemokine/chemokine receptors, since their expression was up-regulated on activated CD8 cells, and susceptibility to ECM was delayed in CCR5-KO mice. Thus, lymphocyte cytotoxicity and cell trafficking are key players in ECM pathogenesis.
- Published
- 2003
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20. Covalent binding of polyethylene glycol to the surface of red blood cells as detected and followed up by cell electrophoresis and rheological methods.
- Author
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Sabolovic D, Sestier C, Perrotin P, Guillet R, Tefit M, and Boynard M
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- Animals, Electrophoresis methods, Erythrocytes parasitology, Female, Humans, Malaria parasitology, Mice, Mice, Inbred C57BL, Rheology methods, Erythrocyte Deformability, Erythrocytes metabolism, Erythrocytes pathology, Malaria blood, Plasmodium, Polyethylene Glycols
- Abstract
Cyanuric chloride activated polyethylene glycol (PEG)-5000 was covalently coupled to murine and human red blood cells (pegylated RBC). Our purpose was to camouflage RBC receptors, which is necessary for parasite invasion, a process essential to sustain parasitemia. Cell electrophoretic mobility analysis (CEM) of pegylated RBC distinguished a new population of cells bearing characteristic CEM. Pegylation of RBC also modified their rheological properties, which were documented by evaluation of cell deformability (based on cell transit time through calibrated micropores) and cell aggregation (as measured by ultrasonic interferometry). Homologous transfusion of pegylated RBC into murine malaria-infected mice had no significant effect on the cerebral malaria death rate in Plasmodium berghei-infected mice, but it reduced the peripheral blood parasitemia by a factor 2 while in Plasmodium yoelii infected mice, the parasitemia was dramatically reduced by a factor of 4. These experiments demonstrate that transfusion of pegylated RBC may inhibit peripheral parasitemia. Cell electrophoresis appears to be a useful tool to allow in vivo detection and to investigate the fate of transfused pegylated RBC.
- Published
- 2000
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