87 results on '"Teague K"'
Search Results
2. Using health policy and systems research to influence national health policies: lessons from Mexico, Cambodia and Ghana
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Strachan, DL, Teague, K, Asefa, A, Annear, PL, Ghaffar, A, Shroff, ZC, McPake, B, Strachan, DL, Teague, K, Asefa, A, Annear, PL, Ghaffar, A, Shroff, ZC, and McPake, B
- Abstract
Health system reforms across Africa, Asia and Latin America in recent decades demonstrate the value of health policy and systems research (HPSR) in moving towards the goals of universal health coverage in different circumstances and by various means. The role of evidence in policy making is widely accepted; less well understood is the influence of the concrete conditions under which HPSR is carried out within the national context and which often determine policy outcomes. We investigated the varied experiences of HPSR in Mexico, Cambodia and Ghana (each selected purposively as a strong example reflecting important lessons under varying conditions) to illustrate the ways in which HPSR is used to influence health policy. We reviewed the academic and grey literature and policy documents, constructed three country case studies and interviewed two leading experts from each of Mexico and Cambodia and three from Ghana (using semi-structured interviews, anonymized to ensure objectivity). For the design of the study, design of the semi-structured topic guide and the analysis of results, we used a modified version of the context-based analytical framework developed by Dobrow et al. (Evidence-based health policy: context and utilisation. Social Science & Medicine 2004;58:207-17). The results demonstrate that HPSR plays a varied but essential role in effective health policy making and that the use, implementation and outcomes of research and research-based evidence occurs inevitably within a national context that is characterized by political circumstances, the infrastructure and capacity for research and the longer-term experience with HPSR processes. This analysis of national experiences demonstrates that embedding HPSR in the policy process is both possible and productive under varying economic and political circumstances. Supporting research structures with social development legislation, establishing relationships based on trust between researchers and policy makers and bu
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- 2023
3. Characterization and evaluation of lactic acid bacteria candidates for intestinal epithelial permeability and Salmonella Typhimurium colonization in neonatal turkey poults
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Yang, Y, Latorre, J D, Khatri, B, Kwon, Y M, Kong, B W, Teague, K D, Graham, L E, Wolfenden, A D, Mahaffey, B D, Baxter, M, Hernandez-Velasco, X, Merino-Guzman, R, Hargis, B M, and Tellez, G
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- 2018
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4. Adapting social prescribing to meet the needs of migrant populations: challenges and solutions to service access and effectiveness
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Kellezi, B, Wakefield, J, Bowe, M, Bridger, K, and Teague, K
- Abstract
Experiences of international migrants in host countries are very diverse. However, many migrants face cumulative challenges to their health and wellbeing which can be best supported by health initiatives aiming to a) address needs derived from socio-political context where they live and b) increase meaningful connection with community. This paper discussed the value and challenges of using one such health initiative to support the needs of vulnerable migration populations: ‘Social Prescribing’. Data was collected from two roundtables with forty participants from organisations working with migrants in the UK and those with lived experience of migration and analysed using thematic analysis. Several challenges to accessing health support were identified including issues around acceptability, appropriateness, and quality of care. Participants emphasised the importance of holistic and culturally appropriate approaches which address health needs while recognising legal, economic and other challenges migrants face. To successfully implement initiatives such as Social Prescribing, there needs to be flexibility, shared understanding between service users and providers, community buy-in, attention to safeguarding and direct involvement of migrants in their development and implementation.
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- 2021
5. Movements of White-Headed and White-Backed Vultures
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Teague K. Scott
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Fishery ,Geography ,White (horse) ,biology ,Ecology (disciplines) ,biology.animal ,Vulture - Abstract
Vultures are the only obligate vertebrate scavengers, and as such provide crucial services as keystone species and support the health and function of ecosystems in which they live. African vultures are a diverse group, with nine species found throughout Sub-Saharan Africa, many with overlapping distributions. Unfortunately, African vultures are faced with numerous threats throughout their range that have led to significant population declines, some greater than 90%, in only three generations. Four of these species are currently listed as critically endangered, and three as endangered. Despite the significant perils faced by African vultures, there are still significant knowledge gaps and, until recently, very little was known about vultures in Mozambique, a large country that falls within the distribution of six of these species. Our research in Gorongosa National Park, Mozambique, focused on movement data collected from 10 White-backed Vultures (Gyps africanus) and 12 White-headed Vultures (Trigonoceps occipitalis), one of Africa’s rarest vulture species. We first focused on estimating White-headed Vulture monthly home ranges and core ranges with continuous-time movement models. We assessed the relationship between ranging behavior and extrinsic (environmental characteristics) and intrinsic (individual characteristics) predictor variables using Bayesian generalized linear mixed effects models. We also explored the degree of White-headed Vulture home range and core range overlap with Gorongosa National Park and its buffer zone. We found that breeding individuals had smaller home ranges and could maintain these into the non-breeding season or abandon them. These small breeding and non-breeding home ranges were representative of central place foraging and averaged 239 km2 and 131 km2, respectively, 80-90% smaller than the average 1180 km2 non-breeding, non-central place foraging home range. Home ranges of birds that used resources outside of the park and its buffer zone were approximately 2.5 times larger than of birds that stayed within park boundaries, suggesting an increase in search effort required to locate less abundant resources. Excursions outside of the park and its buffer zone were rare. Only 15 of the 149 monthly home ranges suggested that birds used resources outside the park; the remaining 134 monthly home ranges fell within 10 km of the edge of the park buffer zone. Additionally, we explored differences in White-headed Vulture and White-backed Vulture movement characteristics. We used Bayesian generalized linear models to determine the effect of species as a predictor for flight altitude, flight speed, onset of movement on two scales (>100 m and >1000 m), and onset of flight at altitude, and the effect of species and hour predictors on hourly activity levels. We found that White-headed Vultures flew at lower altitudes and slower speeds, and initiated movement and flight at altitude earlier than White-backed Vultures. All of these findings correspond with flight less reliant on strong thermals and suggest that the White-headed Vulture is more likely a pioneer than follower. These findings expand on our understanding of both space use by White-headed Vultures and their place within the avian scavenging guild. They also demonstrate the critical importance of protected areas for the survival of the White-headed, and probably other, African Vultures.
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- 2020
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6. A thermodynamic study on cobalt containing calcium ferrite and calcium iron silicate slags at 1573 K
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Teague, K. C., Swinbourne, D. R., and Jahanshahi, S.
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- 2001
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7. Simvastatin and ML141 Decrease Intracellular Streptococcus pyogenes Infection
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Susan A. McDowell, Bria L. Sneed, Katie M. Reed, Nathan C Hahn, Teague K Drinnon, Melissa D Evans, Lindy M. Caffo, Christopher R. Fullenkamp, Heather A. Bruns, Caroline Burcham, John L. McKillip, Robert E. Sammelson, Derron L. Bishop, Olivia Downham, and Samantha Bell
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0301 basic medicine ,Simvastatin ,Streptococcus pyogenes ,030106 microbiology ,Pharmaceutical Science ,CDC42 ,medicine.disease_cause ,Umbilical vein ,Microbiology ,03 medical and health sciences ,Mice ,Streptococcal Infections ,medicine ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,cdc42 GTP-Binding Protein ,Actin ,Cells, Cultured ,Sulfonamides ,biology ,Molecular Structure ,Chemistry ,Macrophages ,Anti-Bacterial Agents ,Fibronectins ,Fibronectin ,030104 developmental biology ,RAW 264.7 Cells ,Fibronectin binding ,biology.protein ,Pyrazoles ,Intracellular ,Biotechnology ,medicine.drug - Abstract
Background :Recurrent pharyngotonsillitis due to Streptococcus pyogenes develops regardless of whether infecting strains are resistant or susceptible to first-line antimicrobials. Causation for recurrent infection is associated with the use of first-line antimicrobials that fail to penetrate deep tissue and host cell membranes, enabling intracellular S. pyogenes to survive throughout repeated rounds of antimicrobial therapy.Objective:To determine whether simvastatin, a therapeutic approved for use in the treatment of hypercholesterolemia, and ML141, a first-in-class small molecule inhibitor with specificity for human CDC42, limit host cell invasion by S. pyogenes.Methods:Assays to assess host cell invasion, bactericidal activity, host cell viability, actin depolymerization, and fibronectin binding were performed using the RAW 267.4 macrophage cell line and Human Umbilical Vein Endothelial Cells (HUVEC) infected with S. pyogenes (90-226) and treated with simvastatin, ML141, structural analogs of ML141, or vehicle control.Results:Simvastatin and ML141 decreased intracellular infection by S. pyogenes in a dose-dependent manner. Inhibition by simvastatin persisted following 1 h washout whereas inhibition by ML141 was reversed. During S. pyogenes infection, actin stress fibers depolymerized in vehicle control treated cells, yet remained intact in simvastatin and in ML141 treated cells. Consistent with the previous characterization of ML141, simvastatin decreased host cell binding to fibronectin. Structural analogs of ML141, designated as the RSM series, decreased intracellular infection through non-cytotoxic, nonbactericidal mechanisms.Conclusion:Our findings demonstrate the potential of repurposing simvastatin and of developing CDC42-targeted therapeutics for eradicating intracellular S. pyogenes infection to break the cycle of recurrent infection through a host-directed approach.
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- 2019
8. cisRED: a database system for genome-scale computational discovery of regulatory elements
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Robertson, G., Bilenky, M., Lin, K., He, A., Yuen, W., Dagpinar, M., Varhol, R., Teague, K., Griffith, O. L., Zhang, X., Pan, Y., Hassel, M., Sleumer, M. C., Pan, W., Pleasance, E. D., Chuang, M., Hao, H., Li, Y. Y., Robertson, N., Fjell, C., Li, B., Montgomery, S. B., Astakhova, T., Zhou, J., Sander, J., Siddiqui, A. S., and Jones, S. J. M.
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- 2006
9. Treatment of lentigo maligna with topical imiquimod
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NAYLOR, M. F., CROWSON, N., KUWAHARA, R., TEAGUE, K., GARCIA, C., MACKINNIS, C., HAQUE, R., ODOM, C., JANKEY, C., and CORNELISON, R. L.
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- 2003
10. Simvastatin and ML141 Decrease Intracellular Streptococcus pyogenes Infection
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Caffo, Lindy, primary, Sneed, Bria L., additional, Burcham, Caroline, additional, Reed, Katie, additional, Hahn, Nathan C., additional, Bell, Samantha, additional, Downham, Olivia, additional, Evans, Melissa D., additional, Fullenkamp, Christopher R., additional, Drinnon, Teague K., additional, Bishop, Derron, additional, Bruns, Heather A., additional, McKillip, John L., additional, Sammelson, Robert E., additional, and McDowell, Susan A., additional
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- 2019
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11. Xylanase Supplementation in Wheat-based Diets and the Influence on Necrotic Enteritis in Broilers: A Translational Model for Human Malnutrition
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Butler L, B.D. Graham, Sami Dridi, Lesleigh Beer, Wilson, Gautier A, and Teague K
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Malnutrition ,medicine ,Xylanase ,General Medicine ,Biology ,medicine.disease ,Microbiology ,Necrotic enteritis - Published
- 2018
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12. Use of probiotics as an alternative to formaldehyde fumigation in commercial broiler chicken hatch cabinets.
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Graham, L E, Teague, K D, Latorre, J D, Yang, Y, Baxter, M F A, Mahaffey, B D, Hernandez-Velasco, X, Bielke, L R, Hargis, B M, and Tellez, G
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BROILER chickens , *PROBIOTICS , *CHICKEN hatcheries , *FORMALDEHYDE , *FUMIGATION - Abstract
Two experiments were conducted in a commercial broiler hatchery to evaluate the use of a spray probiotic formulation as an alternative method to control the bacterial bloom within a broiler hatch cabinet vs. formaldehyde fumigation. In Exp 1, 2 independent trials were conducted to compare hatchery sanitation between the current formaldehyde drip method vs. spray application of the probiotic. Hatchery sanitation was evaluated using the open-plate method at approximately 20% pip; 30% hatch; and 85% hatch for enumeration of total recovered non-selective aerobic bacteria (TAB); presumptive lactic acid bacteria (LAB); and total recovered Gram-negative bacteria (TGB). In Exp 2, 3 independent trials were conducted to evaluate the gastrointestinal (GIT) microbiota of neonatal chicks from hatch cabinets treated as in Exp 1. In Exp 1, in both trials, the application of the probiotic increased the number TAB and LAB present in the hatching environment (P < 0.05). Additionally, at 20% pip and 30% hatch, in both trials, there was no significant difference in TGB levels between the probiotic treatment and the formaldehyde treatment. In Exp. 2, chicks from probiotic treated hatch cabinets also showed a reduction of TGB in the GIT compared to the formaldehyde group (P < 0.05). In trial 3, the reduction in TGB persisted 24 h after hatch. The results of the present study suggest that spray application of a probiotic in commercial hatcheries can yield similar TGB levels when compared to formaldehyde early on in the hatch period. More importantly, it decreased the numbers of these bacteria within the GIT at hatch and 24 h after hatch. [ABSTRACT FROM AUTHOR]
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- 2018
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13. In ovo evaluation of FloraMax®-B11 on Marek's disease HVT vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis infection in broiler chickens.
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Teague, K. D., Graham, L. E., Dunn, J. R., Cheng, H. H., Anthony, N., Latorre, J. D., Menconi, A., Wolfenden, R. E., Wolfenden, A. D., Mahaffey, B. D., Baxter, M., Hernandez-Velasco, X., Merino-Guzman, R., Bielke, L. R., Hargis, B. M., and Tellez, G.
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MAREK'S disease vaccines , *HERPESVIRUS vaccines , *SALMONELLA enteritidis , *BROILER chicken diseases , *HATCHABILITY of eggs - Abstract
Three experiments were conducted to evaluate the effect of in ovo administration of FloraMax®-B11 (FM) on Marek's disease (MD) herpesvirus of turkeys (HVT) vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis (SE) infection in chickens. Experiment 1 consisted of 3 trials. In trials 1 and 2, d 18 White Leghorn 15I5x71 embryos were randomly distributed in 4 groups: 1) HVT vaccinated in ovo and no Marek's disease virus (MDV) challenge; 2), HVT + FM vaccinated in ovo and no MDV challenge; 3) HVT vaccinated in ovo and challenge with virulent MDV (vMDV; strain 583A); and 4), HVT + FM vaccinated in ovo and challenge with vMDV. Trial 3 was designed exactly the same as Experiment 1 but chicks were challenged with very virulent MDV (vvMDV; strains Md5 and 612). Birds were monitored until 8 wk of age, and tested for MD incidence. Experiment 2 consisted of 3 trials. In each trial, d 18 broiler embryos were injected in ovo with either saline or FM to measure hatchability and gastrointestinal bacterial composition. In Experiment 3, d 18 broiler embryos were injected in ovo with either saline or FM. All chickens that hatched were orally gavaged with SE at hatch and kept for 7 d to monitor post-hatch BW. No significant difference (P > 0.05) between MD percentage in birds vaccinated with HVT alone or HVT + FM were observed in Experiment 1. In Experiment 2, probiotic did not negatively affect hatchability, but did reduce lactose positive Gram-negative bacteria. Further, increase in BW was associated with higher villi surface area in the ileum in chickens that received the probiotic as well as a significant reduction in the SE incidence in Experiment 3. These results suggest that in ovo administration of FM does not negatively impact the ability of HVT to protect against MD or hatchability of chickens, but improves BW during the first 7 d of life and decreases SE recovery in chickens. [ABSTRACT FROM AUTHOR]
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- 2017
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14. Fasting and aspiration prophylaxis in labor and for cesarean section
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Teague, K, primary and Dhir, S, additional
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15. Movements of White-Headed and White-Backed Vultures
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Scott, Teague K., primary
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16. A Multilanguage Study of the Quality of Interleaved MELP Voice Traffic Over a Lossy Network
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Harwell, K., primary, Scheets, G., additional, Weber, J., additional, and Teague, K., additional
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- 2009
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17. Breaking ground: A cooperative approach to collecting information on conservation practices from an initially uncooperative population
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Pennington, J. H., primary, Steele, M. A., additional, Teague, K. A., additional, Kurz, B., additional, Gbur, E., additional, Popp, J., additional, Rodriguez, G., additional, Chaubey, I., additional, Gitau, M., additional, and Nelson, M. A., additional
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- 2008
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18. The perceptual quality of melp speech over error tolerant IP networks.
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Gavula, B., Scheets, G., Teague, K., and Weber, J.
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- 2008
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19. A Connectionist Technique for Data Smoothing.
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Daniel, R. and Teague, K.
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- 1990
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20. CARMA Model method of two-dimensional shape classification: An eigensystem approach vs. the LP norm.
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Malakooti, M. and Teague, K.
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- 1987
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21. Chromosome duplications and deletions and their mechanisms of origin.
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Tharapel, A. T., Michaelis, R. C., Velagaleti, G. V. N., Laundon, C. H., Martens, P. R., Buchanan, P. D., Teague, K. E., Tharapei, S. A., Wilroy, R. S., and Jr.
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CHROMOSOME abnormalities ,KARYOTYPES ,CROSSING over (Genetics) ,DNA ,MEIOSIS ,FLUORESCENCE in situ hybridization - Abstract
Duplications and deletions of the same gene loci or chromosome regions are known to produce different clinical manifestations and are significant factors in human morbidity and mortality. Extensive cytogenetic and molecular cytogenetic studies with cosmid and YAC probes in two patients with unique mosaicism for reciprocal duplication-deletion allowed us to further understand the origin of these abnormalities. The first patient's mosaic karyotype was 46,XX,inv dup(11) (q23q13)/46,XX,del(11)(q13q23). The second patient had a 46,XY,dup(7)(p11.2p13)/46,XY,del(7)(p11.2p13)/46,XY karyotype. Fluorescence in situ hybridization studies on the first patient placed the two breakpoints near the folate-sensitive fragile sites FRA11A and FRA11B. The presence of repeated sequences responsible for these fragile sites may have been involved in the patient's duplication-deletion. Our investigation leads us to conclude that, in addition to known mechanisms (such as unequal crossovers between homologs, unequal sister chromatid exchanges, excision of intrachromatid loops, and meiotic recombination within a single chromatid), duplication- deletion can also arise by the formation of an overlying loop followed by an uneven crossover at the level of the DNA strand. [ABSTRACT FROM AUTHOR]
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- 1999
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22. Flow cytometric analysis of the DNA content of non-small cell lung cancer. Ploidy as a significant prognostic indicator in squamous cell carcinoma of the lung.
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Sahin, Aysegul A., Ro, Jae Y., El-Naggar, Adel K., Lee, Jin S., Ayala, Alberto G., Teague, Kim, Hong, Waun K., Sahin, A A, Ro, J Y, el-Naggar, A K, Lee, J S, Ayala, A G, Teague, K, and Hong, W K
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- 1990
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23. Predictive Dynamic Model of a Small Pressure Swing Adsorption Air Separation Unit
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Teague, K. G., Jr. and Edgar, T. F.
- Abstract
A predictive dynamic model of a small pressure swing adsorption (PSA) air separation process was developed for the purposes of evaluation, optimization, and control of oxygen generation systems on board military aircraft. A mathematical model of the adsorption beds was formulated by application of fundamental mass- and energy-transport modeling techniques. These equations were discretized using the Galerkin finite element technique. The resulting ODE systems were coupled with ODEs describing the rate of change of pressure in each bed and models of the feed and exhaust valves and purge orifice. The model was developed so that it is possible to predict the dynamic response of product oxygen composition and feed air consumption to step changes in feed pressure, product flow rate, and cycle time. A laboratory PSA unit similar in size to an on-board oxygen generation system (OBOGS) was constructed to validate the model. The laboratory unit was constructed so that step changes could be implemented and the responses observed for comparison with the model. All parameters in the model were estimated from literature sources with the exception of the feed/exhaust valve and purge orifice discharge coefficients. Excellent dynamic predictions of bed pressure, cycle-averaged feed flow rate, and cycle-averaged bed temperature vs time in response to step changes in all three input variables compared to the two-bed PSA data were achieved without additional parameter estimation from two-bed data.
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- 1999
24. Comparative uptake, metabolism and retention of anthracyclines by tumors growing in vitro and in vivo
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Yesair, D.W., primary, Thayer, P.S., additional, McNitt, S., additional, and Teague, K., additional
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- 1980
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25. cis-Regulatory Element Prediction in Mammalian Genomes
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Siddiqui, A., primary, Robertson, G., additional, Bilenky, M., additional, Astakhova, T., additional, Griffith, O.L., additional, Hassel, M., additional, Lin, K., additional, Montgomery, S., additional, Oveisi, M., additional, Pleasance, E., additional, Robertson, N., additional, Sleumer, M.C., additional, Teague, K., additional, Varhol, R., additional, Zhang, M., additional, and Jones, S., additional
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26. Counter mode encryption for FNBDT/MELP
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Sleezer, R., primary, Daniel, E., additional, Raymond, J., additional, Brewer, J., additional, and Teague, K., additional
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27. CARMA Model method of two-dimensional shape classification: An eigensystem approach vs. the LP norm
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Malakooti, M., primary and Teague, K., additional
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28. A Connectionist Technique for Data Smoothing
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Daniel, R., primary and Teague, K., additional
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29. cis-Regulatory element prediction in mammalian genomes.
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Siddiqui, A., Robertson, G., Bilenky, M., Astakhova, T., Griffith, O.L., Hassel, M., Lin, K., Montgomery, S., Oveisi, M., Pleasance, E., Robertson, N., Sleumer, M.C., Teague, K., Varhol, R., Zhang, M., and Jones, S.
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- 2005
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30. Evaluation of the efficacy of a candidate turkey cellulitis/dermatitis oil emulsion vaccine on immune response and mortality under laboratory and commercial conditions.
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Graham, B D, Robbins, K M, Teague, K D, Graham, L E, Merino-Guzman, R, Tellez, G, and Hargis, B M
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VACCINE effectiveness , *CELLULITIS , *IMMUNE response , *EMULSIONS , *ALUMINUM hydroxide , *SKIN inflammation - Abstract
Ability of a Clostridium septicum (CS) bacterin-toxoid, in conjunction with adjuvants such as aluminum hydroxide, mannoslyated chitosan, or a water-in-oil emulsion Seppic Montanide 71 R VG adjuvant (OE) to induce immunity was evaluated in a 7-wk study (Experiment 1). Poults (20/group) were vaccinated on day of hatch, boosted at 5 wk of age and compared to unvaccinated controls. In Experiment 1, initial vaccination with OE resulted in significantly (P < 0.05) higher antibody levels at 5 wk of age, and at 7 wk of age OE resulted in numerically increased antibody levels compared to all vaccinated groups. OE vaccine efficacy was evaluated in 2 field trials (Experiments 2 and 3) with treatments including a non-vaccinated control group and a vaccinated group. Experiment 2 consisted of 3 houses. Blood samples were obtained at time of field vaccination, 4 wk post-vaccination, and processing. Antibody levels (S/P ratio) in vaccinated groups for weeks 12 and 16 were significantly higher (P < 0.05) than control groups for all houses. Experiment 3 consisted of 6 farms with 1–4 houses/farm. Vaccination significantly (P < 0.05) reduced CS-related mortalities as compared to controls in 5 of 6 farms and antibody titers were significantly (P < 0.05) higher in vaccinated turkeys from 4 wk post-vaccination through processing, for all 6 farms. Based on these results, OE vaccines can be used to increase antibody titers and may reduce CS-related mortalities in the field. [ABSTRACT FROM AUTHOR]
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- 2019
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31. Counter mode encryption for FNBDT/MELP.
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Sleezer, R., Daniel, E., Raymond, J., Brewer, J., and Teague, K.
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- 2002
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32. Sediment-water oxygen and nutrient fluxes in a river-dominated estuary
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Day, Jr., J. W., Teague, K. G., and Madden, C. J.
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- 1988
33. Seasonality of materials transport through a coastal freshwater marsh: riverine versus tidal forcing
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Day, Jr., J. W., Stern, M. K., and Teague, K. G.
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- 1986
34. The Science Performance of JWST as Characterized in Commissioning
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Jane Rigby, Marshall Perrin, Michael McElwain, Randy Kimble, Scott Friedman, Matt Lallo, René Doyon, Lee Feinberg, Pierre Ferruit, Alistair Glasse, Marcia Rieke, George Rieke, Gillian Wright, Chris Willott, Knicole Colon, Stefanie Milam, Susan Neff, Christopher Stark, Jeff Valenti, Jim Abell, Faith Abney, Yasin Abul-Huda, D. Scott Acton, Evan Adams, David Adler, Jonathan Aguilar, Nasif Ahmed, Loïc Albert, Stacey Alberts, David Aldridge, Marsha Allen, Martin Altenburg, Javier Álvarez-Márquez, Catarina Alves de Oliveira, Greg Andersen, Harry Anderson, Sara Anderson, Ioannis Argyriou, Amber Armstrong, Santiago Arribas, Etienne Artigau, Amanda Arvai, Charles Atkinson, Gregory Bacon, Thomas Bair, Kimberly Banks, Jaclyn Barrientes, Bruce Barringer, Peter Bartosik, William Bast, Pierre Baudoz, Thomas Beatty, Katie Bechtold, Tracy Beck, Eddie Bergeron, Matthew Bergkoetter, Rachana Bhatawdekar, Stephan Birkmann, Ronald Blazek, Claire Blome, Anthony Boccaletti, Torsten Böker, John Boia, Nina Bonaventura, Nicholas Bond, Kari Bosley, Ray Boucarut, Matthew Bourque, Jeroen Bouwman, Gary Bower, Charles Bowers, Martha Boyer, Larry Bradley, Greg Brady, Hannah Braun, David Breda, Pamela Bresnahan, Stacey Bright, Christopher Britt, Asa Bromenschenkel, Brian Brooks, Keira Brooks, Bob Brown, Matthew Brown, Patricia Brown, Andy Bunker, Matthew Burger, Howard Bushouse, Steven Cale, Alex Cameron, Peter Cameron, Alicia Canipe, James Caplinger, Francis Caputo, Mihai Cara, Larkin Carey, Stefano Carniani, Maria Carrasquilla, Margaret Carruthers, Michael Case, Riggs Catherine, Don Chance, George Chapman, Stéphane Charlot, Brian Charlow, Pierre Chayer, Bin Chen, Brian Cherinka, Sarah Chichester, Zack Chilton, Taylor Chonis, Mark Clampin, Charles Clark, Kerry Clark, Dan Coe, Benee Coleman, Brian Comber, Tom Comeau, Dennis Connolly, James Cooper, Rachel Cooper, Eric Coppock, Matteo Correnti, Christophe Cossou, Alain Coulais, Laura Coyle, Misty Cracraft, Mirko Curti, Steven Cuturic, Katherine Davis, Michael Davis, Bruce Dean, Amy DeLisa, Wim deMeester, Nadia Dencheva, Nadezhda Dencheva, Joseph DePasquale, Jeremy Deschenes, Örs Hunor Detre, Rosa Diaz, Dan Dicken, Audrey DiFelice, Matthew Dillman, William Dixon, Jesse Doggett, Tom Donaldson, Rob Douglas, Kimberly DuPrie, Jean Dupuis, John Durning, Nilufar Easmin, Weston Eck, Chinwe Edeani, Eiichi Egami, Ralf Ehrenwinkler, Jonathan Eisenhamer, Michael Eisenhower, Michelle Elie, James Elliott, Kyle Elliott, Tracy Ellis, Michael Engesser, Nestor Espinoza, Odessa Etienne, Mireya Etxaluze, Patrick Falini, Matthew Feeney, Malcolm Ferry, Joseph Filippazzo, Brian Fincham, Mees Fix, Nicolas Flagey, Michael Florian, Jim Flynn, Erin Fontanella, Terrance Ford, Peter Forshay, Ori Fox, David Franz, Henry Fu, Alexander Fullerton, Sergey Galkin, Anthony Galyer, Macarena García Marín, Jonathan P. Gardner, Lisa Gardner, Dennis Garland, Bruce Garrett, Danny Gasman, Andras Gaspar, Daniel Gaudreau, Peter Gauthier, Vincent Geers, Paul Geithner, Mario Gennaro, Giovanna Giardino, Julien Girard, Mark Giuliano, Kirk Glassmire, Adrian Glauser, Stuart Glazer, John Godfrey, David Golimowski, David Gollnitz, Fan Gong, Shireen Gonzaga, Michael Gordon, Karl Gordon, Paul Goudfrooij, Thomas Greene, Matthew Greenhouse, Stefano Grimaldi, Andrew Groebner, Timothy Grundy, Pierre Guillard, Irvin Gutman, Kong Q. Ha, Peter Haderlein, Andria Hagedorn, Kevin Hainline, Craig Haley, Maryam Hami, Forrest Hamilton, Heidi Hammel, Carl Hansen, Tom Harkins, Michael Harr, Jessica Hart, Quyen Hart, George Hartig, Ryan Hashimoto, Sujee Haskins, William Hathaway, Keith Havey, Brian Hayden, Karen Hecht, Chris Heller-Boyer, Caroline Henriques, Alaina Henry, Karl Hermann, Scarlin Hernandez, Brigette Hesman, Brian Hicks, Bryan Hilbert, Dean Hines, Melissa Hoffman, Sherie Holfeltz, Bryan J. Holler, Jennifer Hoppa, Kyle Hott, Joseph M. Howard, Rick Howard, Alexander Hunter, David Hunter, Brendan Hurst, Bernd Husemann, Leah Hustak, Luminita Ilinca Ignat, Garth Illingworth, Sandra Irish, Wallace Jackson, Amir Jahromi, Peter Jakobsen, LeAndrea James, Bryan James, William Januszewski, Ann Jenkins, Hussein Jirdeh, Phillip Johnson, Timothy Johnson, Vicki Jones, Ron Jones, Danny Jones, Olivia Jones, Ian Jordan, Margaret Jordan, Sarah Jurczyk, Alden Jurling, Catherine Kaleida, Phillip Kalmanson, Jens Kammerer, Huijo Kang, Shaw-Hong Kao, Diane Karakla, Patrick Kavanagh, Doug Kelly, Sarah Kendrew, Herbert Kennedy, Deborah Kenny, Ritva Keski-kuha, Charles Keyes, Richard Kidwell, Wayne Kinzel, Jeff Kirk, Mark Kirkpatrick, Danielle Kirshenblat, Pamela Klaassen, Bryan Knapp, J. Scott Knight, Perry Knollenberg, Robert Koehler, Anton Koekemoer, Aiden Kovacs, Trey Kulp, Nimisha Kumari, Mark Kyprianou, Stephanie La Massa, Aurora Labador, Alvaro Labiano, Pierre-Olivier Lagage, Charles-Philippe Lajoie, Matthew Lallo, May Lam, Tracy Lamb, Scott Lambros, Richard Lampenfield, James Langston, Kirsten Larson, David Law, Jon Lawrence, David Lee, Jarron Leisenring, Kelly Lepo, Michael Leveille, Nancy Levenson, Marie Levine, Zena Levy, Dan Lewis, Hannah Lewis, Mattia Libralato, Paul Lightsey, Miranda Link, Lily Liu, Amy Lo, Alexandra Lockwood, Ryan Logue, Chris Long, Douglas Long, Charles Loomis, Marcos Lopez-Caniego, Jose Lorenzo Alvarez, Jennifer Love-Pruitt, Adrian Lucy, Nora Luetzgendorf, Peiman Maghami, Roberto Maiolino, Melissa Major, Sunita Malla, Eliot Malumuth, Elena Manjavacas, Crystal Mannfolk, Amanda Marrione, Anthony Marston, André Martel, Marc Maschmann, Gregory Masci, Michaela Masciarelli, Michael Maszkiewicz, John Mather, Kenny McKenzie, Brian McLean, Matthew McMaster, Katie Melbourne, Marcio Meléndez, Michael Menzel, Kaiya Merz, Michele Meyett, Luis Meza, Cherie Miskey, Karl Misselt, Christopher Moller, Jane Morrison, Ernie Morse, Harvey Moseley, Gary Mosier, Matt Mountain, Julio Mueckay, Michael Mueller, Susan Mullally, Jess Murphy, Katherine Murray, Claire Murray, David Mustelier, James Muzerolle, Matthew Mycroft, Richard Myers, Kaila Myrick, Shashvat Nanavati, Elizabeth Nance, Omnarayani Nayak, Bret Naylor, Edmund Nelan, Bryony Nickson, Alethea Nielson, Maria Nieto-Santisteban, Nikolay Nikolov, Alberto Noriega-Crespo, Brian O’Shaughnessy, Brian O’Sullivan, William Ochs, Patrick Ogle, Brenda Oleszczuk, Joseph Olmsted, Shannon Osborne, Richard Ottens, Beverly Owens, Camilla Pacifici, Alyssa Pagan, James Page, Sang Park, Keith Parrish, Polychronis Patapis, Lee Paul, Tyler Pauly, Cheryl Pavlovsky, Andrew Pedder, Matthew Peek, Maria Pena-Guerrero, Konstantin Penanen, Yesenia Perez, Michele Perna, Beth Perriello, Kevin Phillips, Martin Pietraszkiewicz, Jean-Paul Pinaud, Norbert Pirzkal, Joseph Pitman, Aidan Piwowar, Vera Platais, Danielle Player, Rachel Plesha, Joe Pollizi, Ethan Polster, Klaus Pontoppidan, Blair Porterfield, Charles Proffitt, Laurent Pueyo, Christine Pulliam, Brian Quirt, Irma Quispe Neira, Rafael Ramos Alarcon, Leah Ramsay, Greg Rapp, Robert Rapp, Bernard Rauscher, Swara Ravindranath, Timothy Rawle, Michael Regan, Timothy A. Reichard, Carl Reis, Michael E. Ressler, Armin Rest, Paul Reynolds, Timothy Rhue, Karen Richon, Emily Rickman, Michael Ridgaway, Christine Ritchie, Hans-Walter Rix, Massimo Robberto, Gregory Robinson, Michael Robinson, Orion Robinson, Frank Rock, David Rodriguez, Bruno Rodriguez Del Pino, Thomas Roellig, Scott Rohrbach, Anthony Roman, Fred Romelfanger, Perry Rose, Anthony Roteliuk, Marc Roth, Braden Rothwell, Neil Rowlands, Arpita Roy, Pierre Royer, Patricia Royle, Chunlei Rui, Peter Rumler, Joel Runnels, Melissa Russ, Zafar Rustamkulov, Grant Ryden, Holly Ryer, Modhumita Sabata, Derek Sabatke, Elena Sabbi, Bridget Samuelson, Benjamin Sapp, Bradley Sappington, B. Sargent, Arne Sauer, Silvia Scheithauer, Everett Schlawin, Joseph Schlitz, Tyler Schmitz, Analyn Schneider, Jürgen Schreiber, Vonessa Schulze, Ryan Schwab, John Scott, Kenneth Sembach, Clare Shanahan, Bryan Shaughnessy, Richard Shaw, Nanci Shawger, Christopher Shay, Evan Sheehan, Sharon Shen, Allan Sherman, Bernard Shiao, Hsin-Yi Shih, Irene Shivaei, Matthew Sienkiewicz, David Sing, Marco Sirianni, Anand Sivaramakrishnan, Joy Skipper, G. C. Sloan, Christine Slocum, Steven Slowinski, Erin Smith, Eric Smith, Denise Smith, Corbett Smith, Gregory Snyder, Warren Soh, Sangmo Tony Sohn, Christian Soto, Richard Spencer, Scott Stallcup, John Stansberry, Carl Starr, Elysia Starr, Alphonso Stewart, Massimo Stiavelli, Amber Straughn, David Strickland, Jeff Stys, Francis Summers, Fengwu Sun, Ben Sunnquist, Daryl Swade, Michael Swam, Robert Swaters, Robby Swoish, Joanna M. Taylor, Rolanda Taylor, Maurice Te Plate, Mason Tea, Kelly Teague, Randal Telfer, Tea Temim, Deepashri Thatte, Christopher Thompson, Linda Thompson, Shaun Thomson, Tuomo Tikkanen, William Tippet, Connor Todd, Sharon Toolan, Hien Tran, Edwin Trejo, Justin Truong, Chris Tsukamoto, Samuel Tustain, Harrison Tyra, Leonardo Ubeda, Kelli Underwood, Michael Uzzo, Julie Van Campen, Thomas Vandal, Bart Vandenbussche, Begoña Vila, Kevin Volk, Glenn Wahlgren, Mark Waldman, Chanda Walker, Michel Wander, Christine Warfield, Gerald Warner, Matthew Wasiak, Mitchell Watkins, Andrew Weaver, Mark Weilert, Nick Weiser, Ben Weiss, Sarah Weissman, Alan Welty, Garrett West, Lauren Wheate, Elizabeth Wheatley, Thomas Wheeler, Rick White, Kevin Whiteaker, Paul Whitehouse, Jennifer Whiteleather, William Whitman, Christina Williams, Christopher Willmer, Scott Willoughby, Andrew Wilson, Gregory Wirth, Emily Wislowski, Erin Wolf, David Wolfe, Schuyler Wolff, Bill Workman, Ray Wright, Carl Wu, Rai Wu, Kristen Wymer, Kayla Yates, Christopher Yeager, Jared Yeates, Ethan Yerger, Jinmi Yoon, Alice Young, Susan Yu, Dean Zak, Peter Zeidler, Julia Zhou, Thomas Zielinski, Cristian Zincke, Stephanie Zonak, Rigby, J., Perrin, M., Mcelwain, M., Kimble, R., Friedman, S., Lallo, M., Doyon, R., Feinberg, L., Ferruit, P., Glasse, A., Rieke, M., Rieke, G., Wright, G., Willott, C., Colon, K., Milam, S., Neff, S., Stark, C., Valenti, J., Abell, J., Abney, F., Abul-Huda, Y., Scott Acton, D., Adams, E., Adler, D., Aguilar, J., Ahmed, N., Albert, L., Alberts, S., Aldridge, D., Allen, M., Altenburg, M., Alvarez-Marquez, J., Alves de Oliveira, C., Andersen, G., Anderson, H., Anderson, S., Argyriou, I., Armstrong, A., Arribas, S., Artigau, E., Arvai, A., Atkinson, C., Bacon, G., Bair, T., Banks, K., Barrientes, J., Barringer, B., Bartosik, P., Bast, W., Baudoz, P., Beatty, T., Bechtold, K., Beck, T., Bergeron, E., Bergkoetter, M., Bhatawdekar, R., Birkmann, S., Blazek, R., Blome, C., Boccaletti, A., Boker, T., Boia, J., Bonaventura, N., Bond, N., Bosley, K., Boucarut, R., Bourque, M., Bouwman, J., Bower, G., Bowers, C., Boyer, M., Bradley, L., Brady, G., Braun, H., Breda, D., Bresnahan, P., Bright, S., Britt, C., Bromenschenkel, A., Brooks, B., Brooks, K., Brown, B., Brown, M., Brown, P., Bunker, A., Burger, M., Bushouse, H., Cale, S., Cameron, A., Cameron, P., Canipe, A., Caplinger, J., Caputo, F., Cara, M., Carey, L., Carniani, S., Carrasquilla, M., Carruthers, M., Case, M., Catherine, R., Chance, D., Chapman, G., Charlot, S., Charlow, B., Chayer, P., Chen, B., Cherinka, B., Chichester, S., Chilton, Z., Chonis, T., Clampin, M., Clark, C., Clark, K., Coe, D., Coleman, B., Comber, B., Comeau, T., Connolly, D., Cooper, J., Cooper, R., Coppock, E., Correnti, M., Cossou, C., Coulais, A., Coyle, L., Cracraft, M., Curti, M., Cuturic, S., Davis, K., Davis, M., Dean, B., Delisa, A., Demeester, W., Dencheva, N., Depasquale, J., Deschenes, J., Hunor Detre, I., Diaz, R., Dicken, D., Difelice, A., Dillman, M., Dixon, W., Doggett, J., Donaldson, T., Douglas, R., Duprie, K., Dupuis, J., Durning, J., Easmin, N., Eck, W., Edeani, C., Egami, E., Ehrenwinkler, R., Eisenhamer, J., Eisenhower, M., Elie, M., Elliott, J., Elliott, K., Ellis, T., Engesser, M., Espinoza, N., Etienne, O., Etxaluze, M., Falini, P., Feeney, M., Ferry, M., Filippazzo, J., Fincham, B., Fix, M., Flagey, N., Florian, M., Flynn, J., Fontanella, E., Ford, T., Forshay, P., Fox, O., Franz, D., Fu, H., Fullerton, A., Galkin, S., Galyer, A., Garcia Marin, M., Gardner, J. P., Gardner, L., Garland, D., Garrett, B., Gasman, D., Gaspar, A., Gaudreau, D., Gauthier, P., Geers, V., Geithner, P., Gennaro, M., Giardino, G., Girard, J., Giuliano, M., Glassmire, K., Glauser, A., Glazer, S., Godfrey, J., Golimowski, D., Gollnitz, D., Gong, F., Gonzaga, S., Gordon, M., Gordon, K., Goudfrooij, P., Greene, T., Greenhouse, M., Grimaldi, S., Groebner, A., Grundy, T., Guillard, P., Gutman, I., Ha, K. Q., Haderlein, P., Hagedorn, A., Hainline, K., Haley, C., Hami, M., Hamilton, F., Hammel, H., Hansen, C., Harkins, T., Harr, M., Hart, J., Hart, Q., Hartig, G., Hashimoto, R., Haskins, S., Hathaway, W., Havey, K., Hayden, B., Hecht, K., Heller-Boyer, C., Henriques, C., Henry, A., Hermann, K., Hernandez, S., Hesman, B., Hicks, B., Hilbert, B., Hines, D., Hoffman, M., Holfeltz, S., Holler, B. J., Hoppa, J., Hott, K., Howard, J. M., Howard, R., Hunter, A., Hunter, D., Hurst, B., Husemann, B., Hustak, L., Ilinca Ignat, L., Illingworth, G., Irish, S., Jackson, W., Jahromi, A., Jakobsen, P., James, L., James, B., Januszewski, W., Jenkins, A., Jirdeh, H., Johnson, P., Johnson, T., Jones, V., Jones, R., Jones, D., Jones, O., Jordan, I., Jordan, M., Jurczyk, S., Jurling, A., Kaleida, C., Kalmanson, P., Kammerer, J., Kang, H., Kao, S. -H., Karakla, D., Kavanagh, P., Kelly, D., Kendrew, S., Kennedy, H., Kenny, D., Keski-Kuha, R., Keyes, C., Kidwell, R., Kinzel, W., Kirk, J., Kirkpatrick, M., Kirshenblat, D., Klaassen, P., Knapp, B., Scott Knight, J., Knollenberg, P., Koehler, R., Koekemoer, A., Kovacs, A., Kulp, T., Kumari, N., Kyprianou, M., La Massa, S., Labador, A., Labiano, A., Lagage, P. -O., Lajoie, C. -P., Lam, M., Lamb, T., Lambros, S., Lampenfield, R., Langston, J., Larson, K., Law, D., Lawrence, J., Lee, D., Leisenring, J., Lepo, K., Leveille, M., Levenson, N., Levine, M., Levy, Z., Lewis, D., Lewis, H., Libralato, M., Lightsey, P., Link, M., Liu, L., Lo, A., Lockwood, A., Logue, R., Long, C., Long, D., Loomis, C., Lopez-Caniego, M., Lorenzo Alvarez, J., Love-Pruitt, J., Lucy, A., Luetzgendorf, N., Maghami, P., Maiolino, R., Major, M., Malla, S., Malumuth, E., Manjavacas, E., Mannfolk, C., Marrione, A., Marston, A., Martel, A., Maschmann, M., Masci, G., Masciarelli, M., Maszkiewicz, M., Mather, J., Mckenzie, K., Mclean, B., Mcmaster, M., Melbourne, K., Melendez, M., Menzel, M., Merz, K., Meyett, M., Meza, L., Miskey, C., Misselt, K., Moller, C., Morrison, J., Morse, E., Moseley, H., Mosier, G., Mountain, M., Mueckay, J., Mueller, M., Mullally, S., Murphy, J., Murray, K., Murray, C., Mustelier, D., Muzerolle, J., Mycroft, M., Myers, R., Myrick, K., Nanavati, S., Nance, E., Nayak, O., Naylor, B., Nelan, E., Nickson, B., Nielson, A., Nieto-Santisteban, M., Nikolov, N., Noriega-Crespo, A., O'Shaughnessy, B., O'Sullivan, B., Ochs, W., Ogle, P., Oleszczuk, B., Olmsted, J., Osborne, S., Ottens, R., Owens, B., Pacifici, C., Pagan, A., Page, J., Park, S., Parrish, K., Patapis, P., Paul, L., Pauly, T., Pavlovsky, C., Pedder, A., Peek, M., Pena-Guerrero, M., Penanen, K., Perez, Y., Perna, M., Perriello, B., Phillips, K., Pietraszkiewicz, M., Pinaud, J. -P., Pirzkal, N., Pitman, J., Piwowar, A., Platais, V., Player, D., Plesha, R., Pollizi, J., Polster, E., Pontoppidan, K., Porterfield, B., Proffitt, C., Pueyo, L., Pulliam, C., Quirt, B., Quispe Neira, I., Ramos Alarcon, R., Ramsay, L., Rapp, G., Rapp, R., Rauscher, B., Ravindranath, S., Rawle, T., Regan, M., Reichard, T. A., Reis, C., Ressler, M. E., Rest, A., Reynolds, P., Rhue, T., Richon, K., Rickman, E., Ridgaway, M., Ritchie, C., Rix, H. -W., Robberto, M., Robinson, G., Robinson, M., Robinson, O., Rock, F., Rodriguez, D., Rodriguez Del Pino, B., Roellig, T., Rohrbach, S., Roman, A., Romelfanger, F., Rose, P., Roteliuk, A., Roth, M., Rothwell, B., Rowlands, N., Roy, A., Royer, P., Royle, P., Rui, C., Rumler, P., Runnels, J., Russ, M., Rustamkulov, Z., Ryden, G., Ryer, H., Sabata, M., Sabatke, D., Sabbi, E., Samuelson, B., Sapp, B., Sappington, B., Sargent, B., Sauer, A., Scheithauer, S., Schlawin, E., Schlitz, J., Schmitz, T., Schneider, A., Schreiber, J., Schulze, V., Schwab, R., Scott, J., Sembach, K., Shanahan, C., Shaughnessy, B., Shaw, R., Shawger, N., Shay, C., Sheehan, E., Shen, S., Sherman, A., Shiao, B., Shih, H. -Y., Shivaei, I., Sienkiewicz, M., Sing, D., Sirianni, M., Sivaramakrishnan, A., Skipper, J., Sloan, G. C., Slocum, C., Slowinski, S., Smith, E., Smith, D., Smith, C., Snyder, G., Soh, W., Tony Sohn, S., Soto, C., Spencer, R., Stallcup, S., Stansberry, J., Starr, C., Starr, E., Stewart, A., Stiavelli, M., Straughn, A., Strickland, D., Stys, J., Summers, F., Sun, F., Sunnquist, B., Swade, D., Swam, M., Swaters, R., Swoish, R., Taylor, J. M., Taylor, R., Te Plate, M., Tea, M., Teague, K., Telfer, R., Temim, T., Thatte, D., Thompson, C., Thompson, L., Thomson, S., Tikkanen, T., Tippet, W., Todd, C., Toolan, S., Tran, H., Trejo, E., Truong, J., Tsukamoto, C., Tustain, S., Tyra, H., Ubeda, L., Underwood, K., Uzzo, M., Van Campen, J., Vandal, T., Vandenbussche, B., Vila, B., Volk, K., Wahlgren, G., Waldman, M., Walker, C., Wander, M., Warfield, C., Warner, G., Wasiak, M., Watkins, M., Weaver, A., Weilert, M., Weiser, N., Weiss, B., Weissman, S., Welty, A., West, G., Wheate, L., Wheatley, E., Wheeler, T., White, R., Whiteaker, K., Whitehouse, P., Whiteleather, J., Whitman, W., Williams, C., Willmer, C., Willoughby, S., Wilson, A., Wirth, G., Wislowski, E., Wolf, E., Wolfe, D., Wolff, S., Workman, B., Wright, R., Wu, C., Wu, R., Wymer, K., Yates, K., Yeager, C., Yeates, J., Yerger, E., Yoon, J., Young, A., Yu, S., Zak, D., Zeidler, P., Zhou, J., Zielinski, T., Zincke, C., Zonak, S., and bibliotheque, la.
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Science & Technology ,Observatories ,Infrared astronomy ,Astronomical instrumentation ,INFRARED SPECTROGRAPH NIRSPEC ,FOS: Physical sciences ,Astronomy and Astrophysics ,Astronomy & Astrophysics ,Settore FIS/05 - Astronomia e Astrofisica ,Space and Planetary Science ,Physical Sciences ,Astrophysics - Instrumentation and Methods for Astrophysics ,[PHYS.ASTR] Physics [physics]/Astrophysics [astro-ph] ,Instrumentation and Methods for Astrophysics (astro-ph.IM) - Abstract
This paper characterizes the actual science performance of the James Webb Space Telescope (JWST), as determined from the six month commissioning period. We summarize the performance of the spacecraft, telescope, science instruments, and ground system, with an emphasis on differences from pre-launch expectations. Commissioning has made clear that JWST is fully capable of achieving the discoveries for which it was built. Moreover, almost across the board, the science performance of JWST is better than expected; in most cases, JWST will go deeper faster than expected. The telescope and instrument suite have demonstrated the sensitivity, stability, image quality, and spectral range that are necessary to transform our understanding of the cosmos through observations spanning from near-earth asteroids to the most distant galaxies., Comment: 5th version as accepted to PASP; 31 pages, 18 figures; https://iopscience.iop.org/article/10.1088/1538-3873/acb293
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- 2022
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35. Corrigendum: Characterization and evaluation of lactic acid bacteria candidates for intestinal epithelial permeability and Salmonella Typhimurium colonization in neonatal turkey poults.
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Yang, Y, Latorre, J D, Khatri, B, Kwon, Y M, Kong, B W, Teague, K D, Graham, L E, Wolfenden, A D, Mahaffey, B D, and Baxter, M
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SPELLING errors , *PERSONAL names - Abstract
A correction to the article "Characterization and evaluation of lactic acid bacteria candidates for intestinal epithelial permeability and Salmonella Typhimurium colonization in neonatal turkey poults," by Y. Yang and colleagues is presented.
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- 2018
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36. Microplastics absent from reef fish in the Marshall Islands: Multistage screening methods reduced false positives.
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Shaw KR, Whitney JL, Nalley EM, Schmidbauer MC, Donahue MJ, Black J, Corniuk RN, Teague K, Sandquist R, Pirkle C, Dacks R, Sudnovsky M, and Lynch JM
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- Animals, Humans, Plastics chemistry, Environmental Monitoring methods, Fishes, Micronesia, Microplastics, Water Pollutants, Chemical analysis
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Island communities, like the Republic of the Marshall Islands (RMI), depend on marine resources for food and economics, so plastic ingestion by those resources is a concern. The gastrointestinal tracts of nine species of reef fish across five trophic groups (97 fish) were examined for plastics >1 mm. Over 2100 putative plastic particles from 72 fish were identified under light microscopy. Only 115 of these from 47 fish passed a plastic screening method using Fourier-transform infrared microspectroscopy (μFTIR) in reflectance mode. All of these were identified as natural materials in a final confirmatory analysis, attenuated total reflectance FTIR. The high false-positive rate of visual and μFTIR methods highlight the importance of using multiple polymer identification methods. Limited studies on ingested plastic in reef fish present challenging comparisons because of different methods used. No plastic >1 mm were found in the RMI reef fish, reassuring human consumers., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Megan Donahue reports financial support was provided by World Bank Group. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Published by Elsevier Ltd.)
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- 2024
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37. Exploring the role of neuronal-enriched extracellular vesicle miR-93 and interoception in major depressive disorder.
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Burrows K, Figueroa-Hall L, Stewart J, Alarbi A, Kuplicki R, Hannafon B, Tan C, Risbrough V, McKinney B, Ramesh R, Victor T, Aupperle R, Savitz J, Teague K, Khalsa S, and Paulus M
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Major depressive disorder (MDD) is associated with interoceptive processing dysfunctions, but the molecular mechanisms underlying this dysfunction are poorly understood. This study combined brain Neuronal-Enriched Extracellular Vesicle (NEEV) technology and serum markers of inflammation and metabolism with Functional Magnetic Resonance Imaging (fMRI) to identify the contribution of gene regulatory pathways, in particular micro-RNA (miR) 93, to interoceptive dysfunction in MDD. Individuals with MDD ( n = 44) and healthy comparisons (HC; n = 35) provided blood samples and completed an interoceptive attention task during fMRI. EVs were separated from plasma using a precipitation method. NEEVs were enriched by magnetic streptavidin bead immunocapture utilizing a neural adhesion marker (CD171) biotinylated antibody. NEEV specificities were confirmed by ow cytometry, western blot, particle size analyzer, and transmission electron microscopy. NEEV small RNAs were purified and sequenced. Results showed that: (1) MDD exhibited lower NEEV miR-93 expression than HC; (2) within MDD but not HC, those individuals with the lowest NEEV miR-93 expression had the highest serum concentrations of interleukin (IL)-1 receptor antagonist, IL-6, tumor necrosis factor, and leptin; and (3) within HC but not MDD, those participants with the highest miR-93 expression showed the strongest bilateral dorsal mid-insula activation. Since miR-93 is regulated by stress and affects epigenetic modulation by chromatin reorganization, these results suggest that healthy individuals but not MDD participants show an adaptive epigenetic regulation of insular function during interoceptive processing. Future investigations will need to delineate how specific internal and external environmental conditions contribute to miR-93 expression in MDD and what molecular mechanisms alter brain responsivity to body-relevant signals.
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- 2023
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38. Evaluation of the impact of formaldehyde fumigation during the hatching phase on contamination in the hatch cabinet and early performance in broiler chickens.
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Selby CM, Beer LC, Forga AJ, Coles ME, Graham LE, Teague KD, Tellez-Isaias G, Hargis BM, Vuong CN, and Graham BD
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- Animals, Turkeys, Formaldehyde, Escherichia coli, Chickens microbiology, Fumigation
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Commercial hatch cabinet environments promote replication of microorganisms. These pathogenic or apathogenic microorganisms may serve as pioneer colonizers of the gastrointestinal tract (GIT) of poultry. Some of these pioneer colonizers, such as Escherichia coli and Enterococcus spp., are opportunistic pathogens that lead to reduced performance in commercial poultry. Effective hatchery sanitation is imperative to limit contamination of naïve neonatal chicks and poults. Formaldehyde fumigation has been traditionally used to reduce the pathogen load in commercial hatch cabinets. To investigate potential alternatives to formaldehyde fumigation, models to mimic the microbial bloom in a laboratory setting must be utilized. The purpose of the present study was to evaluate the impact of a multispecies environmental challenge model (PM challenge) with and without formaldehyde fumigation during the hatching phase on early performance in broiler chicks. Three experiments were conducted to evaluate microbial contamination in the hatch cabinet environment (air samples, fluff samples), enteric colonization at day-of-hatch (DOH), and 7-day performance. In all experiments, significantly (P < 0.05) more gram-negative bacteria were recovered from the GIT at DOH in the PM challenge control group as compared to the nonchallenged control (NC) group and the formaldehyde-treated group (PM + F). There were no statistical differences in 7-day body weight gain or feed conversion ratio between the PM challenge control group, the NC group or the PM + F group. These data suggest this model could be utilized to evaluate alternatives to formaldehyde fumigation for controlling the microbial load during the hatching phase in a laboratory setting., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2023
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39. Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks.
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Teague KD, Tellez-Isaias G, Chai J, Petrone-Garcia V, Vuong CN, Blanch A, Rasmussen SH, Brown K, Zhao J, and Rochell SJ
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- Animals, Chickens physiology, Fluorescein-5-isothiocyanate, Raffinose pharmacology, Animal Feed analysis, Diet veterinary, Animal Nutritional Physiological Phenomena, Dietary Supplements analysis, Gastrointestinal Microbiome
- Abstract
The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers. Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch. These 5 treatments were administered to 10 replicate cages of 8 birds. Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d. At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage. Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically. Feed conversion ratio increased (P < 0.01) linearly as GOS increased. There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AME
n . There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased. At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased. Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS. Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium. Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2023
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40. Using health policy and systems research to influence national health policies: lessons from Mexico, Cambodia and Ghana.
- Author
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Strachan DL, Teague K, Asefa A, Annear PL, Ghaffar A, Shroff ZC, and McPake B
- Subjects
- Humans, Ghana, Mexico, Cambodia, Health Services Research, Health Policy
- Abstract
Health system reforms across Africa, Asia and Latin America in recent decades demonstrate the value of health policy and systems research (HPSR) in moving towards the goals of universal health coverage in different circumstances and by various means. The role of evidence in policy making is widely accepted; less well understood is the influence of the concrete conditions under which HPSR is carried out within the national context and which often determine policy outcomes. We investigated the varied experiences of HPSR in Mexico, Cambodia and Ghana (each selected purposively as a strong example reflecting important lessons under varying conditions) to illustrate the ways in which HPSR is used to influence health policy. We reviewed the academic and grey literature and policy documents, constructed three country case studies and interviewed two leading experts from each of Mexico and Cambodia and three from Ghana (using semi-structured interviews, anonymized to ensure objectivity). For the design of the study, design of the semi-structured topic guide and the analysis of results, we used a modified version of the context-based analytical framework developed by Dobrow et al. (Evidence-based health policy: context and utilisation. Social Science & Medicine 2004;58:207-17). The results demonstrate that HPSR plays a varied but essential role in effective health policy making and that the use, implementation and outcomes of research and research-based evidence occurs inevitably within a national context that is characterized by political circumstances, the infrastructure and capacity for research and the longer-term experience with HPSR processes. This analysis of national experiences demonstrates that embedding HPSR in the policy process is both possible and productive under varying economic and political circumstances. Supporting research structures with social development legislation, establishing relationships based on trust between researchers and policy makers and building a strong domestic capacity for health systems research all demonstrate means by which the value of HPSR can be materialized in strengthening health systems., (© The Author(s) 2022. Published by Oxford University Press in association with The London School of Hygiene and Tropical Medicine.)
- Published
- 2023
- Full Text
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41. Development of an environmental contamination model to simulate the microbial bloom that occurs in commercial hatch cabinets.
- Author
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Graham BD, Selby CM, Forga AJ, Coles ME, Beer LC, Graham LE, Teague KD, Tellez-Isaias G, Hargis BM, and Vuong CN
- Subjects
- Animals, Bacteria, Egg Shell microbiology, Escherichia coli, Chickens, Ovum
- Abstract
Microbial blooms that emerge in commercial hatch cabinets consist of apathogenic and pathogenic microorganisms, including Escherichia coli, Enterococcus faecalis, and Aspergillus fumigatus. Objectives of the present study included the development of a multipathogen contamination model to mimic commercial conditions and optimization of sampling methods to quantify bacterial or fungal presence within the hatch cabinet. The pathogen challenge mix (PM) was recreated from select bacterial or fungal isolates recovered from an egg homogenate (EH) derived from the contents of infertile eggs and late embryonic mortalities. Isolates selected for PM included Enterococcus faecalis (∼10
8 CFU/egg), Staphylococcus aureus (∼107 CFU/egg), Staphylococcus chromogenes (∼107 CFU/egg), Aspergillus fumigatus (∼106 spores/egg), and 2 Escherichia coli (∼108 CFU/egg) isolates. Challenge (100 μL of PM or EH) was administered using a sterile loop to a 28 mm area on the blunt end of the eggshell at day 19 of embryogenesis (DOE). In 3 experiments, microbiological data were collected from environmental hatcher samples (open-agar plate method), fluff samples, postmortem whole-body chick rinse samples, and gastrointestinal tract (GIT) samples to evaluate select bacteria and fungi circulating within the hatch cabinet and colonization of GIT. Cumulative bacterial and fungal recovery from the PM hatching environment from DOE20 to hatch was higher than the nonchallenged group (NC) and EH group at ∼860 and ∼1,730 CFU, respectively. Bacterial recovery from GIT, fluff, and chick rinse samples were similar for the PM and EH group in Exp. 1. However, Aspergillus fumigatus recovery from fluff and chick rinse samples for the PM group was significantly (P < 0.001) higher than the NC and EH group. In Exp. 2 and 3, PM challenge significantly (P < 0.05) increased Gram-negative bacterial recovery from the GIT, fluff and chick rinse samples compared to both the NC and EH group. These data suggest this innovative multispecies environmental contamination model using PM could be utilized to evaluate strategies to mitigate microbial contamination in commercial hatch cabinets in a laboratory setting., Competing Interests: DISCLOSURES The authors have no conflicts of interest to report., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2022
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42. Executive function and dopamine response in Parkinson's disease freezing of gait.
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Turner TH, Rodriguez-Porcel F, Lee P, Teague K, Heidelberg L, Jenkins S, and Revuelta GJ
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- Aged, Attention drug effects, Female, Gait Disorders, Neurologic etiology, Humans, Male, Middle Aged, Neuropsychological Tests, Parkinson Disease complications, Parkinson Disease psychology, Severity of Illness Index, Task Performance and Analysis, Time and Motion Studies, Treatment Outcome, Antiparkinson Agents therapeutic use, Dopamine therapeutic use, Executive Function drug effects, Gait Disorders, Neurologic psychology, Parkinson Disease drug therapy
- Abstract
Background: This investigation examined whether aspects of attention and executive functioning differed between Parkinson's Disease (PD) patients with freezing of gait (FOG) based on responsiveness to dopamine. We also explored association of cognition with FOG severity and gait metrics., Methods: Fifty-four individuals with PD completed the study protocol: 17 without freezing (PDC), 23 with dopa-responsive FOG (RFOG), and 14 with dopa-unresponsive (URFOG). Standardized neuropsychological tests assessed attention (focused and sustained), psychomotor speed, and set-switching (time and errors). FOG severity was measured using the new FOG Questionnaire (nFOG-Q). Metrics from timed up and go (TUG) tasks were obtained while "on" and "off" dopamine, with and without dual cognitive tasks., Results: After controlling for clinical and demographic factors, analysis of covariance revealed a significant between-group difference for set-switching errors; planned contrasts revealed increased set-switching errors in URFOG relative to RFOG and PD control groups. Groups were not different in other cognitive domains. FOG severity was modestly associated with set-switching errors in RFOG but not URFOG. TUG performances while "on" were associated with set-switching errors in PD controls, and with focused attention in RFOG., Conclusion: PD patients with dopa-unresponsive FOG are more prone to set-switching errors than those who respond to treatment. Furthermore, executive function appears relevant to FOG severity only in patients who show dopamine response. Together, these findings suggest disruption of a common dopamine-mediated pathway for FOG and ability to monitor rules while alternating cognitive processes. Consideration of dopa-response could be useful in characterizing cohorts and treating FOG in PD., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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43. The effect of a mindfulness-based stress intervention on neurobiological and symptom measures in adolescents with early life stress: a randomized feasibility study.
- Author
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Cohen ZP, Cosgrove KT, Akeman E, Coffey S, Teague K, Hays-Grudo J, Paulus MP, Aupperle RL, and Kirlic N
- Subjects
- Animals, Feasibility Studies, Female, Humans, Male, Mice, Psychometrics, Treatment Outcome, Adverse Childhood Experiences psychology, Mindfulness, Stress, Psychological psychology
- Abstract
Background: Early life stress (ELS) has been linked to poor mental and physical health outcomes in adolescence and adulthood. Mindfulness reduces symptoms of depression and anxiety and improves cognitive and social outcomes in both youth and adults. However, little is known whether mindfulness can mitigate against the adverse neurobiological and psychological effects of ELS. This study aimed to examine the feasibility of conducting a group mindfulness intervention in adolescents with ELS and provide preliminary indication of potential effects on stress-related biomarkers and mental health symptoms., Methods: Forty adolescents were randomized to receive either eight sessions of Mindfulness-Based Stress Reduction for Teens in group format (MBSR-T; n = 21) or Treatment as Usual Control group (CTRL; n = 17). Outcomes were assessed at baseline and follow-up and included measures associated with neurobiological functioning (immune and endocrine biomarkers) and self-reported mental health (depressive) symptoms. Linear mixed effects models were used to assess the effects of group and time on these outcome measures., Results: Sixteen of the 21 adolescents completed the intervention, attending an average of 6.5 sessions. The model examining cortisol responses to stress induction revealed medium effects trending toward significance (Cohen's d = .56) for anticipatory cortisol levels in the MBSR-T relative to CTRL groups. No significant effects were found in models examining C-reactive protein or interleukin 6 inflammatory markers. The model examining depressive symptoms revealed a medium effect for symptom reduction (Cohen's d = .69) in the MBSR-T relative to CTRL groups., Conclusions: This study demonstrated feasibility of conducting a group-based MBSR-T intervention for adolescents with ELS. There was some evidence for efficacy on a symptom level with potential subtle changes on a biological level. Future larger studies are needed to determine the efficacy of group-based mindfulness interventions in this population., Trial Registration: Identifier # NCT03633903 , registered 16/08/2018.
- Published
- 2021
- Full Text
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44. Research Note: Application of an Escherichia coli spray challenge model for neonatal broiler chickens.
- Author
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Selby CM, Graham BD, Graham LE, Teague KD, Hargis BM, Tellez-Isaias G, and Vuong CN
- Subjects
- Animals, Animals, Newborn microbiology, Bacteria isolation & purification, Escherichia coli physiology, Bacterial Infections microbiology, Bacterial Infections transmission, Bacterial Infections veterinary, Chickens, Disease Models, Animal, Poultry Diseases microbiology, Poultry Diseases transmission
- Abstract
Avian pathogenic Escherichia coli (E. coli) is an opportunistic pathogen often introduced to neonatal chicks during the hatching process. This commensal bacterium, particularly as a pioneer colonizer of the gastrointestinal tract, can have substantial implications in the rearing of poultry because of reduced flock performance. In order to mimic the effects of the natural bacterial bloom present during the hatch, a seeder challenge model was developed to expose neonatal chicks to virulent E. coli. On day 20 of embryogenesis, selected early hatched chicks (n = 18/hatcher) were briefly removed and sprayed challenged with saline (vehicle) or E. coli at 1 × 10
7 colony-forming unit (CFU)/chick (exp 1) and 2.5 × 107 CFU/chick (exp 2). These challenged chicks were returned to the hatcher to serve as seeders to transmit the pathogen to the indirect challenged or contact chicks (n = 195/hatcher). For two 7-d experiments, the efficacy of transmission was evaluated via enteric bacterial recovery, body weight gain (BWG), and mortality. For exp 1 and exp 2, significantly (P < 0.0001) more gram-negative bacteria were recovered from the seeder and contact gastrointestinal samples than the negative control samples on day of hatch. In addition, there was a reduction (P < 0.05) in 7-d BWG and significantly (P < 0.0001) higher mortality in the contact-challenged chicks than the negative control chicks in both exp 1 and exp 2. These data suggest that this challenge model could be used to evaluate different methods of controlling the bacterial bloom that occurs in the hatching environment., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2021
- Full Text
- View/download PDF
45. Development of a wild-type Escherichia coli environmental bloom model to evaluate alternatives to formaldehyde fumigation in broiler chicken hatch cabinets.
- Author
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Graham BD, Selby CM, Graham LE, Teague KD, Tellez-Isaias G, Hargis BM, and Vuong CN
- Subjects
- Animals, Formaldehyde, Fumigation, Models, Biological, Chickens, Disinfection methods, Escherichia coli physiology, Ovum microbiology
- Abstract
Horizontal transmission of opportunistic Escherichia coli during hatch can have detrimental effects on early performance, particularly as pioneer colonizers. Commercially, formaldehyde is often applied in the United States to combat the bacterial bloom that occurs inside of the hatching environment. The purpose of these experiments was to develop a replicable E. coli horizontal challenge model to evaluate alternatives to formaldehyde sanitation applied to the hatching environment. In experiment 1, two trials were conducted for 2 wild-type (WT) E. coli isolates (isolate 1 [I1] or isolate 2 [I2]) to determine the appropriate in ovo challenge dose and day of embryogenesis (DOE) for challenge administration. In experiment 1 trial 1, the most appropriate inoculation dose and time point were determined to be 10
2 cfu/embryo on DOE 19. Experiment 1 trial 2 evaluated whether placement of seeder (direct-challenged) embryos with contact (indirect-challenged) embryos during hatch affected contact hatchability. Trial 2 showed no differences in hatchability between groups. A 7-day experiment (experiment 1 trial 2) was conducted to evaluate the effects of I1 or I2 on horizontal transmission, gram-negative bacterial (GNB) recovery from the gastrointestinal tract (GIT), and impact on BW gain (BWG). Compared with the negative control, seeder, and contact chicks challenged with I1 or I2, we observed increased (P < 0.05) GNB recovered from GIT on the day of hatch. There was a marked (P < 0.05) reduction in 7-day BWG between the I1 indirect-challenged group and the negative control group. To further validate the model, 2 7-day trials (experiment 2, experiment 3) were conducted to evaluate the effects of formaldehyde fumigation on coliform recovery from the hatching environment and on early performance using I1 for the challenge. Isolate 1 positive control hatchers had increased levels of circulating coliforms compared with the negative control and formaldehyde-treated hatchers, although there was no significant impact on performance induced by challenge or formaldehyde treatment in experiment 2 or experiment 3. These data provide a potential model for investigations related to horizontal transmission of WT E. coli at a low dose on DOE 19 to promote simulated commercially relevant bacterial blooms under laboratory conditions., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2021
- Full Text
- View/download PDF
46. Electronic Nicotine Delivery Systems and Serum Folate: A Case Study.
- Author
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Croff JM, Chiaf AL, Hartwell ML, Crockett EK, Tan C, and Teague K
- Abstract
Introduction: In 2018, the US Surgeon General declared youth electronic nicotine delivery systems (ENDS) use as an epidemic. Combustible cigarettes have been shown to adversely affect folate status; however, no study to date has explored how ENDS impact folate status., Methods: In this case study, a white 18-year-old woman was followed for a 1-month period as part of a larger study. During her participation in the study, a self-report of dietary folate consumption and use of alcohol, tobacco, and other drugs (ATOD) was collected. Each week, dried blood spots were collected to assess red blood cell (RBC) and serum folate., Results: During the first 2 weeks of the study, she used ENDS and her serum folate values were depleted in the ranges of 1.91 to 4.39 µg/L. During the third week, when no ENDS were used, her serum folate value was measured at 29.44 µg/L. When ENDS use resumed during the fourth week, her serum folate value fell to 7.50 µg/L., Conclusions: This relationship suggests the need for additional studies on ENDS use and serum folate status, particularly among adolescent women who already have the lowest folate status nationally., Competing Interests: Declaration of conflicting interests:The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2019.)
- Published
- 2019
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47. Isolation and characterization of a Rickettsia from the ovary of a Western black-legged tick, Ixodes pacificus.
- Author
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Alowaysi M, Chen J, Stark S, Teague K, LaCourse M, Proctor J, Vigil K, Corrigan J, Harding A, Li J, Kurtti T, and Zhong J
- Subjects
- Animals, Bacterial Typing Techniques, California, DNA, Intergenic, Female, Multilocus Sequence Typing, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, DNA, Bacterial genetics, Ixodes microbiology, Ovary microbiology, Rickettsia classification, Rickettsia isolation & purification
- Abstract
A rickettsial isolate was obtained from a partially engorged Ixodes pacificus female, which was collected from Humboldt County, California. The isolate was provisionally named Rickettsia endosymbiont Ixodes pacificus (REIP). The REIP isolate displayed the highest nucleotide sequence identity to Rickettsia species phylotype G021 in I. pacificus (99%, 99%, and 100% for ompA, 16S rRNA, and gltA, respectively), a bacterium that was previously identified in I. pacifiucs by PCR. Analysis of sequences from complete opening frames of five genes, 16S rRNA, gltA, ompA, ompB, and sca4, provided inference to the bacteria's classification among other Rickettsia species. The REIP isolate displayed 99.8%, 99.4%, 99.2%, 99.5%, and 99.6% nucleotide sequence identity for 16S rRNA, gltA, ompA, ompB, and sca4 gene, respectively, with genes of 'R. monacensis' str. IrR/Munich, indicating the REIP isolate is closely related to 'R. monacensis'. Our suggestion was further supported by phylogenetic analysis using concatenated sequences of 16S rRNA, gltA, ompA, ompB, and sca4 genes, concatenated sequences of dksA-xerC, mppA-purC, and rpmE-tRNA
fMet intergenic spacer regions. Both phylogenetic trees implied that the REIP isolate is most closely related to 'R. monacensis' str. IrR/Munich. We propose the bacterium be considered as 'Rickettsia monacensis' str. Humboldt for its closest phylogenetic relative (=DSM 103975 T = ATCC TSD-94 T)., (Copyright © 2019 Elsevier GmbH. All rights reserved.)- Published
- 2019
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48. Alternative expression analysis by RNA sequencing.
- Author
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Griffith M, Griffith OL, Mwenifumbo J, Goya R, Morrissy AS, Morin RD, Corbett R, Tang MJ, Hou YC, Pugh TJ, Robertson G, Chittaranjan S, Ally A, Asano JK, Chan SY, Li HI, McDonald H, Teague K, Zhao Y, Zeng T, Delaney A, Hirst M, Morin GB, Jones SJ, Tai IT, and Marra MA
- Subjects
- Antimetabolites, Antineoplastic pharmacology, Cell Line, Tumor, Colorectal Neoplasms drug therapy, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, Databases, Genetic, Drug Resistance, Neoplasm genetics, Expressed Sequence Tags, Fluorouracil pharmacology, Gene Expression drug effects, Gene Expression Profiling, Humans, Oligonucleotide Array Sequence Analysis, Protein Isoforms, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Alternative Splicing, RNA, Messenger genetics, Sequence Analysis, RNA methods
- Abstract
In alternative expression analysis by sequencing (ALEXA-seq), we developed a method to analyze massively parallel RNA sequence data to catalog transcripts and assess differential and alternative expression of known and predicted mRNA isoforms in cells and tissues. As proof of principle, we used the approach to compare fluorouracil-resistant and -nonresistant human colorectal cancer cell lines. We assessed the sensitivity and specificity of the approach by comparison to exon tiling and splicing microarrays and validated the results with reverse transcription-PCR, quantitative PCR and Sanger sequencing. We observed global disruption of splicing in fluorouracil-resistant cells characterized by expression of new mRNA isoforms resulting from exon skipping, alternative splice site usage and intron retention. Alternative expression annotation databases, source code, a data viewer and other resources to facilitate analysis are available at http://www.alexaplatform.org/alexa_seq/.
- Published
- 2010
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49. Radiologic placement of central venous catheters: rates of success and immediate complications in 3412 cases.
- Author
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Tseng M, Sadler D, Wong J, Teague KR, Schemmer DC, Saliken JC, So B, and Gray RR
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Alberta, Cross-Sectional Studies, Female, Humans, Male, Middle Aged, Punctures instrumentation, Quality Assurance, Health Care, Risk, Ultrasonography instrumentation, Arteries injuries, Catheterization, Central Venous instrumentation, Embolism, Air epidemiology, Hemorrhage epidemiology, Pneumothorax epidemiology, Radiology, Interventional instrumentation
- Abstract
Objective: To determine the success and immediate complication rates associated with 3412 central venous catheter placements performed in an interventional radiology suite., Methods: Success and immediate complication rates were prospectively recorded for 3412 consecutive patients who had central venous catheters radiologically placed at a tertiary care centre between July 1993 and October 2000. The indication for placement and the insertion site were also recorded., Results: The most common indication for both short- and long-term venous access was hemodialysis, and the right internal jugular vein was the most common site for catheter insertion. Placement was successful for 98.8% of tunnelled lines and 99.3% of temporary catheters. The rate for immediate complications (including pneumothorax, air emboli, bleeding and arterial puncture) for tunnelled catheter placements was 3.8% and for temporary catheter placements was 1.6%; no major complications were documented., Conclusion: Our results lend further evidence to the claim that the success and immediate complication rates of radiologically placed central venous catheters compare favourably with blind placement and surgical placement of central venous catheters.
- Published
- 2001
50. Recurrent fetal cystic hygroma with normal chromosomes: case report and review of the literature.
- Author
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Teague KE, Eggleston MK, Muffley PE, and Gherman RB
- Subjects
- Adult, Amniocentesis, Female, Fetal Death, Gestational Age, Humans, Karyotyping, Male, Pregnancy, Recurrence, Ultrasonography, Prenatal, Lymphangioma, Cystic diagnostic imaging, Lymphangioma, Cystic genetics
- Abstract
Recurrence of fetal cystic hygroma in subsequent pregnancies is extremely rare. A review of the literature to date revealed only two other reports of recurrence with normal fetal karyotypes documented in at least two of the affected pregnancies. At 11 weeks' gestation, the fetus of a 19-year-old gravida 3 para 0 was discovered to have a large cystic hygroma. Subsequent evaluation during the second trimester revealed increasing size of the septated nuchal mass and ascites. A 46,XX fetal karyotype was noted in her two prior pregnancies, both of which had also been complicated by the development of cystic hygroma and nonimmune hydrops. Cystic hygroma, associated with a normal karyotype, can be inherited as an autosomal recessive trait.
- Published
- 2000
- Full Text
- View/download PDF
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