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34 results on '"Taylor, Mark E."'

3. A Medicago truncatula Tobacco Retrotransposon Insertion Mutant Collection with Defects in Nodule Development and Symbiotic Nitrogen Fixation

Author

Pislariu, Catalina I., Murray, Jeremy D., Wen, JiangQi, Cosson, Viviane, Muni, RajaSekhara Reddy Duvvuru, Wang, Mingyi, Benedito, Vagner A., Andriankaja, Andry, Cheng, Xiaofei, Jerez, Ivone Torres, Mondy, Samuel, Zhang, Shulan, Taylor, Mark E., Tadege, Million, Ratet, Pascal, Mysore, Kirankumar S., Chen, Rujin, and Udvardi, Michael K.

Published
2012

25. Microbiological Control of Eurasian Watermilfoil

Author

MASSACHUSETTS UNIV AMHERST DEPT OF ENVIRONMENTAL SCIENCES, Gunner, Haim B., Limpa-Amara, Yuthana, Bouchard, Beryl S., Weilerstein, Philip J., Taylor, Mark E., MASSACHUSETTS UNIV AMHERST DEPT OF ENVIRONMENTAL SCIENCES, Gunner, Haim B., Limpa-Amara, Yuthana, Bouchard, Beryl S., Weilerstein, Philip J., and Taylor, Mark E.

Abstract

An attempt was made to develop an ecosystems approach for the control of the nuisance aquatic plant Eurasian watermilfoil, Myriophyllum spicatum L. To this end, a cellulolytic fungus, Mycoleptodiscus terrestris (Gerdemann) Ostazeski (M.t.), and a pectinolytic bacterium, Bacillus sp. strain P8 (BSP8), were isolated from the microbial populations naturally resident in the phyllosphere of this plant. These organisms grew compatibly with each other, were able to compete successfully with other microflora on the plant surface, and were able to resist the inhibitory action of phenolic compounds produced by the plants. Application of these organisms to the plant, after growth in appropriate media, resulted in plant decline and eventual death. The process of decline included hormone-like stress effects on the plant induced by BSP8 (internodal elongation), and increase in strongly pectinolytic microbial populations associated with the plant, and penetration into plant tissue by the fungal mycelium. Specificity trials of M. t. infectivity revealed it to be very weakly pathogenic to several aquatic species and terrestrial plants, and thereby without potential significant impact outside the area of designated application.

Published
1990

31. A Medicago truncatula Tobacco Retrotransposon Insertion Mutant Collection with Defects in Nodule Development and Symbiotic Nitrogen Fixation1[W][OA].

Author

Pislariu, Catalina I., Murray, Jeremy D., Wen, JiangQi, Cosson, Viviane, Muni, RajaSekhara Reddy Duvvuru, Wang, Mingyi, Benedito, Vagner A., Andriankaja, Andry, Cheng, Xiaofei, Jerez, Ivone Torres, Mondy, Samuel, Zhang, Shulan, Taylor, Mark E., Tadege, Million, Ratet, Pascal, Mysore, Kirankumar S., Chen, Rujin, and Udvardi, Michael K.

Subjects
MEDICAGO truncatula, RETROTRANSPOSONS, NITROGEN fixation, POLYMERASE chain reaction, ALLELES
Abstract

A Tntl-insertion mutant population of Medicago truncatula ecotype R108 was screened for defects in nodulation and symbiotic nitrogen fixation. Primary screening of 9,300 mutant lines yielded 317 lines with putative defects in nodule development and/or nitrogen fixation. Of these, 230 lines were rescreened, and 156 lines were confirmed with defective symbiotic nitrogen fixation. Mutants were sorted into six distinct phenotypic categories: 72 nonnodulating mutants (Nod-), 51 mutants with totally ineffective nodules (Nod+ Fix-), 17 mutants with partially ineffective nodules (Nod+ Fix+/-), 27 mutants defective in nodule emergence, elongation, and nitrogen fixation (Nod+/- Fix-), one mutant with delayed and reduced nodulation but effective in nitrogen fixation (dNod+/- Fix+), and 11 supernodulating mutants (Nod++Fix+/ ). A total of 2,801 flanking sequence tags were generated from the 156 symbiotic mutant lines. Analysis of flanking sequence tags revealed 14 insertion alleles of the following known symbiotic genes: NODULE INCEPTION (NIN), DOESN'T MAKE INFECTIONS3 (DMI3 / CCaMK), ERE REQUIRED FOR NODULATION, and SUPERNUMERARY NODULES (SUNN). In parallel, a polymerase chain reactionbased strategy was used to identify Tntl insertions in known symbiotic genes, which revealed 25 additional insertion alleles in the following genes: DMI1, DMI2, DMI3, NIN, NODULATION SIGNALING PATHWAY1 (NSP1), NSP2, SUNN, and SICKLE. Thirty-nine Nod- lines were also screened for arbuscular mycorrhizal symbiosis phenotypes, and 30 mutants exhibited defects in arbuscular mycorrhizal symbiosis. Morphological and developmental features of several new symbiotic mutants are reported. The collection of mutants described here is a source of novel alleles of known symbiotic genes and a resource for cloning novel symbiotic genes via Tntl tagging. [ABSTRACT FROM AUTHOR]

Published
2012
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34. A Medicago truncatula Tobacco Retrotransposon Insertion Mutant Collection with Defects in Nodule Development and Symbiotic Nitrogen Fixation1[W][OA].

Author

Pislariu, Catalina I., Murray, Jeremy D., Wen, JiangQi, Cosson, Viviane, Muni, RajaSekhara Reddy Duvvuru, Wang, Mingyi, Benedito, Vagner A., Andriankaja, Andry, Cheng, Xiaofei, Jerez, Ivone Torres, Mondy, Samuel, Zhang, Shulan, Taylor, Mark E., Tadege, Million, Ratet, Pascal, Mysore, Kirankumar S., Chen, Rujin, and Udvardi, Michael K.

Subjects
*MEDICAGO truncatula, *RETROTRANSPOSONS, *NITROGEN fixation, *POLYMERASE chain reaction, *ALLELES
Abstract

A Tntl-insertion mutant population of Medicago truncatula ecotype R108 was screened for defects in nodulation and symbiotic nitrogen fixation. Primary screening of 9,300 mutant lines yielded 317 lines with putative defects in nodule development and/or nitrogen fixation. Of these, 230 lines were rescreened, and 156 lines were confirmed with defective symbiotic nitrogen fixation. Mutants were sorted into six distinct phenotypic categories: 72 nonnodulating mutants (Nod-), 51 mutants with totally ineffective nodules (Nod+ Fix-), 17 mutants with partially ineffective nodules (Nod+ Fix+/-), 27 mutants defective in nodule emergence, elongation, and nitrogen fixation (Nod+/- Fix-), one mutant with delayed and reduced nodulation but effective in nitrogen fixation (dNod+/- Fix+), and 11 supernodulating mutants (Nod++Fix+/ ). A total of 2,801 flanking sequence tags were generated from the 156 symbiotic mutant lines. Analysis of flanking sequence tags revealed 14 insertion alleles of the following known symbiotic genes: NODULE INCEPTION (NIN), DOESN'T MAKE INFECTIONS3 (DMI3 / CCaMK), ERE REQUIRED FOR NODULATION, and SUPERNUMERARY NODULES (SUNN). In parallel, a polymerase chain reactionbased strategy was used to identify Tntl insertions in known symbiotic genes, which revealed 25 additional insertion alleles in the following genes: DMI1, DMI2, DMI3, NIN, NODULATION SIGNALING PATHWAY1 (NSP1), NSP2, SUNN, and SICKLE. Thirty-nine Nod- lines were also screened for arbuscular mycorrhizal symbiosis phenotypes, and 30 mutants exhibited defects in arbuscular mycorrhizal symbiosis. Morphological and developmental features of several new symbiotic mutants are reported. The collection of mutants described here is a source of novel alleles of known symbiotic genes and a resource for cloning novel symbiotic genes via Tntl tagging. [ABSTRACT FROM AUTHOR]

Published
2012
Full Text
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