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Previously, we have found that the use of visible and near-infrared (VIS-NIR) spectroscopy is a useful nondestructive method for quantification of β-cryptoxanthin in Satsuma mandarin as an alternative method to HPLC. In this study, we examined whether the use of VIS-NIR spectroscopy would permit the quantification of β- cryptoxanthin in mid-late ripening citrus cultivars. Examination of the associations between VIS-NIR spectra data and β-cryptoxanthin contents was performed using partial least squares regression (PLSR). We found that the absorption in the region of 600 nm is particularly important for estimating the β-cryptoxanthin content, and that the wavelength regions related to sugar content also affect the assessment of β-cryptoxanthin. From these results, it is suggested that the use of VIS-NIR spectroscopy could allow prediction of β-cryptoxanthin content in mid-late ripening citrus cultivars. [ABSTRACT FROM AUTHOR]