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4 results on '"Tatlidede, Elif"'

1. Sıçanlarda Doksorubisin'in neden olduğu kardiyak toksisite ve hemodinamik değişikliklere karşı Resveratrol'ün koruyucu etkilerinin araştırılması

Author

Tatlidede, Elif, Şener, Göksel, and Farmakoloji Anabilim Dalı

Subjects
Pharmacy and Pharmacology, Doxorubicin, Oxidative damage, Apoptosis, Eczacılık ve Farmakoloji, Cardiomyopathies
Abstract

Bu çalışmada doksorubisinin neden olduğu kardiyotoksisite ve hemodinamik değişiklikler incelenerek, resveratrolün bu değişiklikler üzerindeki olası koruyucu etkilerinin biyokimyasal ve histolojik yöntemlerle araştırılması amaçlanmıştır. Bu amaçla Wistar albino sıçanlardan 4 grup oluşturuldu. İlk gruptaki sıçanlara sadece serum fizyolojik, ikinci gruba resveratrol (RVT), diğer gruplara doksorubisin (DOKS) ve DOKS + RVT uygulandı. Serum fizyolojik ve RVT, DOKS uygulamasından 2 hafta önce uygulanmaya başlandı. DOKS uygulaması sonrasında hayvanlar 3 hafta gözlendi. Deney başlangıcı ve sonunda tüm gruplardaki hayvanların kan basıncı, kalp hızları ve vücut ağırlıkları kaydedildi. 7 hafta sonunda sıçanlar dekapite edilerek kan ve doku (kalp) örnekleri alındı. Tüm gruplardan alınan kan örneklerinde sitokin (İL-1beta, TNF-alfa), laktat dehidrojenaz (LDH), kreatin kinaz (KK), aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), total antioksidan kapasite (AOC), 8-hidroksi-2'-deoksiguanozin (8-OHdG), trigliserid (TG), total kolesterol (TC), kan üre azotu (BUN) ve kreatinin, kalp dokusundan alınan örneklerde glutatyon (GSH), malondialdehit (MDA) düzeyleri, miyeloperoksidaz (MPO) ve Na+/K+-ATPaz aktiviteleri ile kollajen içeriği tayin edildi. Sonuçlar incelendiğinde DOKS grubunda bulunan sıçanların kalp hızları ve kan basınçlarının kontrol grubuna göre anlamlı olarak düştüğü, DOKS + RVT grubunda ise bu düşüşün önlendiği görüldü. Serumda yapılan incelemelerde DOKS uygulamasının incelenen biyokimyasal parametrelerde meydana getirdiği değişikliklerin RVT tedavisi ile anlamlı olarak düzeldiği gözlendi. Benzer şekilde kalp dokusunda artan malondialdehit ve kollajen düzeyleri ile miyeloperoksidaz aktivitesinin, azalan Na+/K+-ATPaz aktivitesi ve glutatyon düzeylerinin ise RVT tedavisi ile geri çevrildiği ve kontrol değerlere yaklaştığı belirlendi. Bu sonuçlar, resveratrol uygulamasının doksorubisinin neden olduğu değişiklikleri önleyerek doksorubisine bağlı kalp yetmezliği gelişen hastaların yaşam kalitesini düzeltebileceğini ve mortalite riskini azaltabileceğini düşündürmektedir. The aim of this study was to investigate the possible protective role of resveratrol on doxorubicin-induced cardiotoxicity and hemodynamic changes using biochemical and histological parameters. Wistar albino rats were divided into four groups. First group was administered saline only and served as control. The second group was treated with resveratrol (RVT); and the other groups were treated with doxorubicin (DXR) or DXR plus RVT, respectively. RVT or saline treatment was done 2 weeks before DXR administration. Rats were observed after a 3-week post-treatment period. Blood pressure, heart rate and body weight were monitored for all groups at the begining and at the end of experiment. After 7 weeks rats were decapitated. Trunk blood was taken for the determination of cytokines (IL-1beta, TNF-alpha), lactate dehydrogenase (LDH), creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total antioxidant capacity (AOC), 8-hydroxy-2'-deoxyguanosine (8-OHdG), triglyceride (TG), total cholesterol (TC), blood urea nitrogen (BUN) and creatinine, while the heart samples were obtained to measure glutathione (GSH), malondialdehyde (MDA) and collagen levels, myeloperoxidase (MPO) and Na+/K+-ATPase activity. In saline-treated DXR group, there were alterations in all of the studied biochemical parameters which were also reversed with RVT treatment. Furthermore, DOX treatment causes increase in the heart MDA, MPO and collagen content while GSH and Na+/K+-ATPase activities were decreased. On the other hand, these DXR-induced biochemical changes in the heart tissue were reversed significantly with RVT treatment. The present results indicate that RVT administration could prevent DXR-induced changes and ameliorate quality of life in patients with DXR-induced heart failure and reduce mortality. 82

Published
2008

3. Melatonin improves cardiovascular function and ameliorates renal, cardiac and cerebral damage in rats with renovascular hypertension.

Author

Erşahin, Mehmet, Şehirli, Özer, Toklu, Hale Z., Süleymanoglu, Selami, Emekli-Alturfan, Ebru, Yarat, Ayşen, Tatlidede, Elif, Yeğen, Berrak C., and Şener, Göksel

Subjects
MELATONIN, ANGIOTENSINS, RENAL hypertension, GLUTATHIONE, OXIDATIVE stress, LABORATORY rats
Abstract

The effect of melatonin was investigated in an angiotensin II-dependent renovascular hypertension model in Wistar albino rats by placing a renal artery clip (two-kidney, one-clip; 2K1C), while sham rats did not have clip placement. Starting either on the operation day or 3 wk after the operation, the rats received melatonin (10 mg/kg/day) or vehicle for the following 6 wk. At the end of the nineth week, after blood pressure (BP) and echocardiographic recordings were obtained, plasma samples were obtained to assay lactate dehydrogenase (LDH), creatine kinase (CK), antioxidant capacity (AOC), asymmetric dimethylarginine (ADMA), and nitric oxide (NOx) levels. In the kidney, heart and brain tissues, malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO) and Na+-K+ ATPase activities were determined. 2K1C caused an increase in BP and left ventricular (LV) dysfunction. In hypertensive animals LDH, CK, ADMA levels were increased in plasma with a concomitant reduction in AOC and NOx. Moreover, hypertension caused a significant decrease in tissue SOD, CAT, and Na+, K+-ATPase activities and glutathione content, while MDA levels and MPO activity were increased in all studied tissues. On the other hand, both melatonin regimens significantly reduced BP, alleviated oxidative injury and improved LV function. In conclusion, melatonin protected against renovascular hypertension-induced tissue damage and improved cardiac function presumably due to both its direct antioxidant and receptor-dependent actions, suggesting that melatonin may be of therapeutic use in preventing oxidative stress due to hypertension. [ABSTRACT FROM AUTHOR]

Published
2009
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4. Antioxidant effect of alpha-lipoic acid against ethanol-induced gastric mucosal erosion in rats.

Author

Sehirli O, Tatlidede E, Yüksel M, Erzik C, Cetinel S, Yeğen BC, and Sener G

Subjects
Animals, Antioxidants therapeutic use, Female, Gastric Mucosa pathology, Lipid Peroxidation drug effects, Lipid Peroxidation physiology, Male, Rats, Rats, Wistar, Reactive Oxygen Species metabolism, Stomach Ulcer chemically induced, Stomach Ulcer drug therapy, Stomach Ulcer metabolism, Thioctic Acid therapeutic use, Antioxidants pharmacology, Ethanol toxicity, Gastric Mucosa drug effects, Gastric Mucosa metabolism, Thioctic Acid pharmacology
Abstract

Background/aims: This investigation elucidates the role of free radicals in ethanol-induced gastric mucosal erosion and the protective effect of lipoic acid., Methods: After overnight fasting, Wistar albino rats were orally treated with 1 ml of absolute ethanol to induce gastric erosion. Lipoic acid (100 mg/kg) was given orally for 3 days before ethanol administration. Mucosal damage was evaluated 1 h after ethanol administration by macroscopic examination and histological analysis. Additional tissue samples were taken for measurement of malondialdehyde, glutathione (GSH), and myeloperoxidase activity. Production of reactive oxidants and oxidant-induced DNA fragmentation and Na(+),K(+)-ATPase activity were also assayed in the tissue samples., Results: Generation of reactive oxygen species and lipid peroxidation associated with neutrophil infiltration play an important role in the pathogenesis of gastric mucosal damage induced by ethanol. Furthermore, oxidants depleted tissue GSH stores and impaired membrane structure as Na(+),K(+)-ATPase activity was inhibited. On the other hand, lipoic acid treatment reversed all these biochemical indices as well as the histopathological changes induced by ethanol., Conclusion: These data suggest that lipoic acid administration effectively counteracts the deleterious effect of ethanol-induced gastric mucosal injury and attenuates gastric damage through its antioxidant effects., ((c) 2008 S. Karger AG, Basel.)

Published
2008
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