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ABSTRACT Safflower is a crop with seeds containing high amounts of oleic and linoleic acids, which have applications in cosmetics, food, feed, and pharmaceutical industries. Safflower seeds are generally reported to undergo after-ripening and to have physiological dormancy. This study comprehensively analyzed the physical changes and the induction of dormancy and germinability in developing seeds to determine whether the safflower population is adequately developed during seed dispersal. From the 80th day after flowering (DAF), seeds were collected separately from plants on a weekly basis until 131 DAF, resulting in seven sampling dates. The changes in length, diameter, thickness, water content, and fresh and dry weight during seed development were measured. Simultaneously, the effects of dark and light, temperature, and gibberellic acid (GA3) (0, 50, and 100 µM) on seed germination, dormancy and germination characteristics of safflower seeds were investigated. The seeds ceased to increase in length, width and thickness, reaching a mature appearance at 131 DAF, with a dry mass per seed of 0.0443 g. The water content decreased between 117 and 124 DAF, indicating an obvious process of desiccation during the final stage of seed maturation. Germination capacity is acquired at 117 DAF, as evidenced by an increase in germination and a reduction in dormant seeds, particularly at low temperatures (10°C) and with GA3 supplementation. At 131 DAF, seeds exhibited 7.9 % water content, and there was an increase in seed germination and a decrease in seed dormancy status, regardless of GA3 supplementation and temperature.

ABSTRACT The high quality of soybean seeds is essential for the success of the crop, as it determines the field performance of seedlings. Seed physiological attributes are associated with genotype stability; thus, the maintenance of genome integrity is crucial to ensure seed quality. To evaluate the quality of soybean seed lots at physiological and molecular levels, a completely randomized design was carried out in four replications, with 12 soybean seed lots (cv. BMX Potência RR). Seeds were evaluated through standard germination and vigor tests. Genomic DNA was extracted from the root meristem of seeds from different lots with the Kasvi Spin 50 ® extraction kit. The analysis was performed through electrophoresis and visualized under ultraviolet (UV) light. The Comet assay was also applied to investigate DNA damage. Soybean seed lots exhibited differences in germination and vigor, as determined by the tests of accelerated aging, field emergence, emergence speed index, shoot length, root length, shoot fresh weight, root fresh mass, and shoot dry mass. In seeds with low germination and vigor, DNA damage and genotoxicity were observed. DNA from samples with high germination and vigor observed by the field emergence and accelerated aging tests was more preserved than DNA extracted from seeds with low physiological quality.

Abstract: Knowledge of the genetic variability and of the variables for evaluating common bean lines for the physiological seed quality trait is important for the selection of promising common bean genotypes for this trait in breeding programs. Through multivariate analysis, the objective of this study was to evaluate the efficiency of physical and physiological tests for studies of genetic variability in seeds of common bean lines. Twenty lines of common bean were evaluated for thousand-seed weight, first count, germination, tetrazolium, electrical conductivity, accelerated aging, field emergence and seedling performance. The experimental data were subjected to analysis of variance, analysis of genetic parameters and multivariate analysis of principal components. Genetic variability was observed among the common bean lines, and two groups of lines with superior physiological performance according to the germination and seed vigor tests were identified. First germination count, germination, seedling length in the field, accelerated aging and emergence speed index tests are the most promising for characterizing the physiological potential of seeds of common bean lines.

Sunn hemp (Crotalaria spectabilis)is widely used for soil correction and cover, but it is still necessary to know the characteristics that influence the storage of its seeds. This study aimed to evaluate the physiological quality of sunn hemp seeds under uncontrolled environmental conditions and in acclimatization chamber. A completely randomized experimental design was used, with four replicates, in subplots evaluated in different periods. Freshly harvested seeds and seeds at 90, 180 and 270 days of storage were evaluated for germination, first germination count, germination speed index, seedling emergence in the field, accelerated aging, cold test and seedling growth. The environment and storage time significantly influenced the seed germination and vigor. The seeds showed a high physiological performance for both the environmental conditions, maintaining the ability to produce normal seedlings up to approximately 150 days of storage, deteriorating afterwards, although without losing their germination capacity up to 270 days of storage.

Crotalaria crops have important uses, such as in green manuring, nematode control, biological nitrogen fixation and sugarcane reform in Savanna areas. Due to its strategic importance, knowledge about crotalaria seed production technology is a relevant factor to ensure the availability of high physiological quality seeds. This study aimed to assess the effect of two row spacings (20 and 40 cm) on the germination and vigor of Crotalaria ochroleuca and Crotalaria spectabilis seeds produced in two crop seasons (2018 and 2019). The experimental design was randomized blocks, with four replications, in a 2 x 2 factorial scheme (spacing x crop season), for each species. The climatic conditions during seed production significantly influenced the physiological quality of the crotalaria seeds. The 2018 harvest, with more abundant rainfall and milder temperatures during flowering, provided more vigorous seeds for both species. The 20 cm spacing was more efficient to produce C. ochroleuca seeds with high physiological performance. Notwithstanding, C. spectabilisseeds showed a higher physiological quality when produced under 40 cm spacing than when produced under denser cultivation.

Soybean represents a valuable source of food for humans and animals and the quality of the seeds has great importance for the establishment and high productivity of this crop. Soybean seeds require continuous improvement, which is dependent on a better understanding of the regulatory mechanisms that coordinate seed germination. To investigate whether the method of water absorption into soybean seeds could lead to abnormal seedlings, and if this could be associated with cytogenetical consequences, we premoistened the seeds of three cultivars; M 6410 IPRO, M6210 IPRO, and BMX Potência RR by direct immersion (fast method), use of a wet substrate (intermediate method), and by moist atmosphere (slow method) with distilled water at 25ºC for 24 hours. We investigated the normal and abnormal seedlings, electrical conductivity, mitosis, cell death, and the chromosomal abnormalities index. The comet assay was applied to investigate DNA fragmentation. Direct immersion in water induced seedling growth inhibition and caused cytological alterations associated with genotoxicity effects in the studied soybean genotypes. Slow premoistening of the seeds increased seedling performance as a result of higher final germination percentage (above 85%), reduced abnormal seedlings (below 5%, on average), and reduced the electrical conductivity of seeds. All three genotypes of soybean seeds lost their ability to withstand the imbibitional damage induced by direct immersion as abnormal seedlings increased. We concluded that the fast water absorption by seeds poses a threat to genomic integrity owing to its potential for genotoxicity to DNA, manifesting as breaks or loss of whole chromosomes. Slow premoistening of the seeds resulted in a longer time period to deal with damage. Stabilized seedling growth was provided by altering cytogenetic responses during uptake of water by soybean seeds through the maintenance of cell viability.

Abstract: The air temperature used in the drying process can determine the initial physiological quality and storage potential of a seed lot, which is the object of this study. Safflower seeds, harvested at a moisture content of 25.8%, were subjected to drying in an experimental dryer at air temperatures of 40, 50, 60 and 70 °C until reaching a moisture content of 6.6 ± 0.6%. Immediately upon drying and every 60 days after that, up to 240 days of storage under uncontrolled conditions, seed samples were collected to determine physiological quality. Increasing drying air temperature resulted in higher water removal rates, promoting immediate and latent damage to seed physiological quality, evidenced by the reduction in the percentage and speed of root protrusion, first count, and germination, mainly at temperatures of 60 and 70 °C. For the maintenance of safflower seed germination and vigor up to 240 days of storage, it is recommended that drying air temperature does not exceed 40 °C, especially when seeds present moisture contents close to or higher than 25.8% at the drying moment.

ABSTRACT: Relationships among water uptake rate and temperatures were investigated in five soybean seeds lots cv. M 6410 IPRO. Germination, field emergence and electrical conductivity tests were applied to determine seed performance after fast (complete immersion in water), control (moist substrate) and slow (moist atmosphere) imbibitions both at 20 °C and 30 °C. The fresh weight increment over time in soybean seeds during imbibition was recorded. In parallel, analysis of the DNA integrity of seedlings obtained from such seeds was performed in gel electrophoresis. Absorption pattern of soybean seeds were similar among temperatures, but absorption characteristics vary with time progress according to the way of water entrance into the seeds. Upon fast imbibition seeds germinated lower than non-fast-imbibed seeds, besides loss of seed performance. In contrast, slow-imbibed seeds showed high germination, low abnormal seedlings and maintenance of seeds performance. DNA isolation from fast imbibed seeds was highly degraded; although, some considerably degraded samples were reported in controlled imbibed seeds and the best preserved DNA was found in slow-imbibed seeds. The pattern of DNA degradation is typically passive or non programmed cell death. Our results showed it is important to consider the water uptake rate during germination test, since DNA integrity plays a critical role during seed imbibition, preserving soybean seed performance.

Abstract: The productivity of the oilseed rape crop is responsive to the application of nitrogen (N) and the effect of N is sulfur (S)-dependent. In addition, storage may influence seed vigor. The aim of the present study was to assess the effect of fertilization with nitrogen and sulfur on the physiological quality of rapeseeds. All combinations of four doses of N (0, 40, 80, and 120 kg.ha-1) and five of S (0, 30, 60, 90, and 120 kg.ha-1) were evaluated with four replications each distributed in a randomized block design in a distroferric Red Latosol soil. The effects of the nutrients and their combinations on the physiological potential of rapeseeds were evaluated even immediately after harvest and 180 days of storage through the germination test, accelerated aging, field emergence and seedling performance. The application of N associated with S had a positive effect on the physiological quality of the seeds. The application of the 120 kg.ha-1 dose of N and the 80 kg.ha-1 dose of S promoted the highest germination and vigor of the seeds. Seed storage for a period of 180 days led to a drastic reduction in the physiological potential of the rapeseeds.

Determination of seed-maturation indicators enables the identification of the ideal moment for harvest to achieve the best production and conservation potential. Our objective here was to evaluate some physical and physiological changes of crambe (Crambe abyssinica Hochst) seeds as possible indicators of seed maturation. Crambe flowering was monitored in Dourados, Mato Grosso do Sul, Brazil. Plants were tagged, and 13, 22, 26, and 28 days after the initiation of flowering, the seeds were collected and following physical attributes evaluated: length, diameter, total mass, dry matter and water content. Physiological quality of the seeds was assessed using the germination test, by registering the percentage of normal seedlings and dormant seeds, immediately after each harvest, and again after six months of storage. The water-absorption curves were characterized as a function the seed-development stages. All physical attributes were observed to increase because of the accumulation of reserve substances during seed development, except for water content, which gradually decreased from 72.2% at the start of development to 29.5% at maturity. At 28 days after anthesis the germination percentage of crambe seeds at physiological maturity was only 17%, indicating that they became dormant while maturing. However, seed germination rate was 89% after six months of storage, indicating that dormancy was almost fully overcome after this period.

Drying agricultural products reduces the moisture content to suitable levels for storage, in order to maintain the product quality. However, special care with the temperatures applied in the process is important for the integrity and longevity of the material. The present study aimed at determining the immediate and latent effect of air-drying temperatures on the quality of soybean produced as a second season crop. The grains were collected at the R8 stage, close to the physiological maturity, with moisture content of approximately 23 % (w.b.), submitted to drying temperatures of 40 ºC, 50 ºC, 60 ºC, 70 ºC and 80 ºC, up to a moisture content of 12.5 ± 0.7 % (w.b.), and then stored under non-controlled humidity and temperature for 180 days. Thereafter, quality was assessed every 45 days by determining the dry matter loss, color and crude protein and lipid contents, as well as the acidity and peroxide indices of the crude oil extracted. Based on the results obtained, it was concluded that the increase in the air-drying temperature affects the soybean quality and crude oil extracted, being this effect enhanced with the storage time; the soybean and crude oil quality decline with an increase in the air-drying temperature and storage time; the air temperature of 40 ºC has the least effect on the quality of soybean grains and crude oil extracted.

Abstract: This study was performed to evaluate the immediate and latent effects of drying air temperature on the physiological potential of peanuts (Arachis hypogaea L.). The fruits were harvested at the physiological maturity stage, with 36 ± 2% water content (w.b.), and dried at temperatures of 40, 50, 60 and 70 ºC, until 7 ± 1% (w.b.). Thereafter, the fruits were stored in an environment with non-controlled temperature and relative humidity for 150 days, and the water content, germination and vigor of seeds were immediately determined, as well as at every 30 days thereafter, through the first count, and the tetrazolium test, accelerated aging test, cold test with soil and electrical conductivity. According to the results, it was found that the physiological potential of peanut seeds is inversely related to the drying temperature and the storage time, due to the fact that the germination and vigor are reduced as both factors increase. The air temperature of 40 °C may be recommended for the drying of peanut seeds, and even associated with the storage time of 150 days under uncontrolled conditions.

Abstract During germination, orthodox seeds lose their ability to tolerate desiccation resembling recalcitrant seeds. This research aimed to investigate the cell changes during the re-induction of the desiccation tolerance (DT) in Sesbania virgata germinated seeds with 1, 3 and 5 mm long radicles. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moisture content) followed by rehydration. Cell viability was assessed through TUNEL test in dry radicles and transmission electron microscopy in both fresh and dry radicles. The positive-TUNEL confirmed the DNA degradation, through the green fluorescence of the cell nuclei from 5 mm radicle length and the ultra structural evaluations detected loss of cellular content integrity in 3 and 5 mm cell radicles that did not survive dehydration to 10%.

O crambe ainda não é uma cultura amplamente cultivada, no entanto, tem despertado o interesse dos produtores, pois todo seu cultivo é mecanizado, apresenta baixo custo de produção, pode ser utilizada em rotação de culturas e tem aptidão para a produção de biodiesel. Os dados sobre resposta a adubação nessa cultura são escassos, sendo assim, objetivou-se com este trabalho avaliar as características morfológicas e a produtividade do crambe em função da aplicação de diferentes doses de nitrogênio em cobertura, utilizando-se como fonte de nitrogênio a ureia. O experimento foi realizado em condições de campo, no ano de 2010, e o delineamento utilizado foi em blocos casualizados, com cinco tratamentos e seis repetições. Os tratamentos constituíram-se da aplicação de 0; 5; 10; 20 e 40 kg ha-1 de nitrogênio em cobertura. Para os teores de clorofila nas folhas e para a massa de mil grãos, a aplicação de doses crescentes de nitrogênio em cobertura aumentou significativamente os valores. Para os parâmetros morfológicos avaliados, como comprimentos de parte aérea e área foliar e para a produtividade da cultura do crambe, os tratamentos utilizados não foram significativos.

Niger is an oleaginous species whose cultivation has been spreading, but there is not much information on the adverse conditions during its seedling establishment. This study aimed at evaluating the effects of substrate water availability and seed water content on niger germination. Seeds were moistened using the humid atmosphere method for 0; 24; 48; and 72 hours, obtaining the water contents of 7.0 %, 12.8 %, 16.8 % and 32.2 %. Then, they were sown in substrate moistened with PEG 6000 solutions with different osmotic potentials: 0.0 MPa (control), -0.1 MPa, -0.2 MPa, -0.3 MPa and -0.4 MPa. A completely randomized design, in a 4 x 5 factorial scheme (water content x osmotic potential), with four replications of 50 seeds, was used. First count and germination percentage, germination speed index and mean time, shoot and root length and seedlings dry weight were evaluated. The reduction in the substrate osmotic potential decreases the niger seed germination and seedling growth, regardless of water content, but with a higher evidence in seed water contents below 32.2 % and 12.8 %, respectively.

This research aimed to investigate the cellular alterations during the loss and re-establishment of desiccation tolerance (DT) in germinated Sesbania virgata seeds. The loss of DT was characterized in germinated seeds with increasing radicle lengths (1, 2, 3, 4 and 5 mm) when subjected to dehydration in silica gel, followed by rehydration. To re-establish DT, the germinated seeds were incubated for 72h in polyethylene glycol (PEG, -2.04 MPa) with or without ABA (100 μM) before dehydration in silica gel. Cell viability was assessed by seedling survival, and DNA integrity was evaluated by gel electrophoresis. Seeds with 1 mm radicle length survived dehydration to the original moisture content (MC) of the dry seed (approximately 10%). PEG treatment was able to re-establish DT, at least partially, with 2, 3 and 4 mm but not in 5 mm radicle lengths. Germinated seeds treated with PEG+ABA performed better than those treated only with PEG, and DT was re-established even in germinated seeds with a 5 mm radicle length. Among the PEG-treated germinated seeds dehydrated to 10% MC, DNA integrity was maintained only in those with a 1 mm radicle length.

A dificuldade técnica devida à ocorrência de dormência das sementes é um entrave para a produção de mudas de espécies nativas em programas de recomposição de áreas degradadas do Cerrado. Objetivou-se com este trabalho avaliar o efeito do condicionamento fisiológico e do sombreamento na qualidade de sementes de duas espécies de barbatimão (Stryphnodendron polyphyllum e S. adstringens). Foram utilizados os seguintes tratamentos pré-germinativos: 1) PEG -1,0 MPa; 2) PEG -1,0 MPa+ KNO3 -1,0 MPa; 3) PEG -0,5 MPa; 4) PEG -0,5 MPa + KNO3 -0,5 MPa; 5) KNO3 -1,0 MPa; 6) Água deionizada; e 7) sementes que não receberam nenhum tratamento pré-germinativo (testemunha). Durante os tratamentos pré-germinativos, as sementes permaneceram incubadas em B.O.D. regulada na alternância de 20/30 ºC, durante 24 h, e em seguida semeadas em bandejas de células com Plantmax® mantidas em quatro níveis de sombreamento (pleno sol, 30%, 50% e 70%) com quatro repetições de 20 sementes. O condicionamento fisiológico com água de sementes de S. polyphyllum e S. adstringens e posterior semeadura em ambiente sombreado de 50 a 70% são eficientes para obter emergência rápida e elevada, maior altura e massa seca das plântulas. As sementes de S. polyphyllum podem ser osmocondicionadas com PEG 1,0 e PEG -0,5 MPA se a semeadura for realizada sob 30% de sombra. Entretanto, a semeadura de S. polyphyllum e S. adstringens não deve ser realizada a pleno sol.

Water stress and seed water content can affect the first germination stages and disturb the full pasture establishment. This study aimed at evaluating the effect of substrate water availability and seed water content on the germination of Urochloa ruziziensis seeds, as well as verifying any harmful effect of seed imbibition. The treatments consisted of seeds with 9.5%, 11.3% and 14.6% of moisture content, subjected to substrate water availability simulated with aqueous solutions of polyethylene glycol 6000 (PEG 6000) of 0.0 MPa (PubMed control), -0.1 MPa, -0.2 MPa, -0.3 MPa and -0.4 MPa. The following characteristics were used to evaluate the seeds physiological quality: germination percentage and first counting, aerial and primary root length and seedlings fresh weight. The experimental design was completely randomized, with four replications of 50 seeds. All the evaluated characteristics were negatively influenced by the water potentials reduction, however, the U. ruziziensis seeds with 11.3% and 14.6% of moisture content showed a higher germination rate and seedlings growth at low water availability. Despite the reduction in the indexes evaluated for water deficiency, the damage extension in seeds was determined by seeds moisture content and water stress severity.

O interesse pelo crambe (Crambeabyssinica) surgiu em virtude da sua superioridade, em relação à soja e às demais oleaginosas, na produção de óleos vegetais, e por adaptar-se com facilidade ao plantio direto. Objetivou-se, com este trabalho, avaliar o armazenamento de sementes de crambe, em diferentes embalagens e ambientes. Foram testadas as embalagens de saco de polietileno e embalagens de plástico rígido com fechamento hermético, nas seguintes condições: ambiente (25 ± 2 ºC e 60% de UR) e câmara fria e seca (15 ± 2 ºC e 45 % de UR), durante 0, 30, 60, 90, 120, 150 e 180 dias (período de maio a novembro de 2010), sendo avaliados, mensalmente, o teor de água, a germinação, na primeira contagem, o índice de velocidade de germinação e o tempo médio de germinação das sementes. O experimento foi conduzido em DIC, com quatro repetições de 50 sementes para cada tratamento. Inicialmente, as sementes apresentaram germinação média de 60% e teor de água de 7,0%. As embalagens e ambientes empregados permitiram alterações no teor de água que desencadearam o início da deterioração das sementes, evidenciado pela redução de germinação na primeira contagem, índice de velocidade e aumento do tempo médio de germinação das sementes. Entretanto, a germinação não foi influenciada pelos tratamentos e períodos de armazenamento. De maneira geral, as sementes de crambe podem ser acondicionadas em embalagem impermeável durante 180 dias de armazenamento, em condição de15 ± 2 ºC e 45% de UR.

The knowledge on biometric aspects of fruits and seeds and their influence on germination are important for the production and properties of native species. This study aimed at evaluating the influence of fruit and seed size on the germination and vigor of Campomanesia adamantium seeds. For the biometric characterization, 120 fruits were randomly selected and visually divided into four classes, with predominance for size, in which longitudinal and transversal diameters and mass of seeds were measured. The seed characterization was carried out in 30 fruits of each class, by determining the number of whole, empty and total seeds of every fruit. The seeds physiological potential, according to fruit size, was evaluated with the aid of the following tests: imbibition curve, moisture content, germination percentage and speed index, seedling length, total fresh and dry matter of seedlings. A completely randomized design, with four treatments, was used. Fruits were respectively classified according to their longitudinal and transversal diameters as small (12.71 mm; 12.46 mm), medium small (15.38 mm; 15.01 mm), medium large (18.84 mm; 18.02 mm) and large (22.74 mm; 22.36 mm). The classes of small, medium small and medium large fruits presented seeds with higher germination percentage and speed index, while seedlings with higher biomass accumulation were observed for large fruits.

The obtaining of commercial cultivars of potato with resistance to the phytopatogen is a very promising alternative that the genetic engineering can provide, through the introduction of exogenous genes for the genetic transformation. In order to establish an efficient system of transformation, it is crucial first to optimize in vitro organogenesis protocol. This work aimed to establish an efficient protocol for the genetic transformation of potato cv. Atlantic. The efficiency of the in vitro organogenesis is influenced by the explant type and by the kind and concentrations of growth regulators used. Shoot segments of potato cv. Atlantic present better organogenesis capacity than leaf explants. It is recommended to use the woody plant medium (WPM) supplied with 1,0 mg L-1 of naphthalene acetic acid (NAA) + 5,0 mg L-1 of zeatin riboside (ZEA), to obtain shoots from shoot segments, and the carbenicilin (Cb) added to this medium increased this formation. For the process to enlarge shoots, this same antibiotic when added to the WPM medium in growing concentrations (100; 250; 500 mg L-1) promoted a decrease in the height of plants, number of shoots and length of roots. After the co-cultivation of shoots segments with Agrobacterium tumefaciens these concentrations of Cb are not efficient in the elimination of the bacteria, which commits the organogenesis. However, it is possible to establish a protocol of in vitro organogenesis of potato cv. Atlantic starting from shoots segments not co-cultivated. These results will be useful for futures experiments of genetic transformation with this and another commercials cultivars of potato.A obtenção de cultivares comerciais de batata com resistência a fitopatógenos é uma alternativa muito promissora que a engenharia genética pode proporcionar, através da introdução de genes exógenos visando transformação genética. Para tal, torna-se imprescindível estabelecer um sistema eficiente de transformação, e neste sentido a determinação de um protocolo de organogênese in vitro é o passo fundamental para este processo. Objetivou-se determinar um protocolo eficiente para a transformação genética de batata cv. Atlantic. A eficiência da organogênese in vitro é influenciada pelo tipo de explante e pelo tipo e concentrações de reguladores de crescimento utilizados. Segmentos internodais de batata cv. Atlantic apresentam melhor capacidade organogenética que explantes foliares. Recomenda-se utilizar o meio woody plant medium (WPM) suplementado com 1,0 mg L-1 de ácido naftaleno-acético (ANA) + 5,0 mg L-1 de zeatina ribosídeo (ZEA), para se obter brotações em segmentos internodais, sendo que a carbenicilina (Cb) adicionada a este meio potencializa esta formação. Para o processo de alongamento de brotos, este mesmo antibiótico quando adicionado ao meio WPM em concentrações crescentes (100; 250; 500 mg L-1) promove uma diminuição na altura de plantas, número de nós e comprimento radicular. Após o co-cultivo de segmentos internodais com Agrobacterium tumefaciens estas concentrações de Cb não são eficientes na eliminação da bactéria, o que compromete a organogênese. No entanto, é possível estabelecer um protocolo de organogênese in vitro de batata cv. Atlantic a partir de segmentos internodais não co-cultivados. Estes resultados serão úteis para futuros experimentos de transformação genética com esta e outras cultivares comerciais de batata.

This article aims at evaluating the effects of different packaging and varied storage temperatures on the germination potential of seeds of Campomanesia adamantium Camb. O. Berg. The seeds were packaged in glass, aluminum foil and plastic containers, or maintained inside intact fruits at 5, 10 and 15 ºC during 0, 7, 14 and 21 days. After these periods the seeds were sown in Germitest® germination paper and maintained in incubation chambers at 25 ºC under constant white light for 42 days. Seed moisture contents were evaluated both before and after storage, as well as germination percentages, germination speed index, root and aerial portion of seedlings lengths, and total dry weights. All possible combinations of packing materials, temperatures and storage times were tested, with four repetitions of 25 seeds for each treatment. C. adamantium seeds showed initial water contents of 31.5%. Glass and aluminum packaging were efficient at maintaining the water content of the seeds, and provided greater germination speed index than the other packaging materials. Germination percentages, seedlings lengths and dry weights did not vary among the different temperatures tested. C. adamantium seeds can be stored for up to 21 days at temperatures between 5 and 15 ºC without altering their physiological quality. In terms of cost-benefit efficiencies, these seeds can be stored without significant damage for 21 days while still inside the fruits at temperatures of 5, 10 or 15 ºC.Objetivou-se com este trabalho avaliar o efeito de diferentes embalagens e temperaturas durante o armazenamento sobre o potencial de germinação das sementes de Campomanesia adamantium Camb. O. Berg. As sementes foram mantidas em embalagens de vidro, papel de alumínio, plástico e no interior do fruto, nas temperaturas de 5, 10 e 15 ºC, durante zero, sete, quatorze e 21 dias. Após esse período, as sementes foram semeadas em rolo de papel Germitest® e mantidas em B.O.D. a 25 ºC e luz branca constante durante 42 dias. Antes e após o armazenamento foram avaliados o teor de água das sementes, porcentagem e índice de velocidade de germinação, comprimento de raiz, parte aérea e total e a massa seca das plântulas. Todas as combinações de embalagens, temperaturas e tempos de armazenamento foram testadas com quatro repetições de 25 sementes para cada tratamento. Sementes de C. adamantium apresentam 31,5% de teor de água após o processamento. As embalagens de vidro e de alumínio foram eficientes para manter o teor de água das sementes, proporcionando maior velocidade de germinação em relação aos demais ambientes. A porcentagem de germinação, comprimento e massa seca de plântulas não variaram entre as temperaturas testadas. Sementes de C. adamantium podem ser armazenadas por até 21 dias nas temperaturas entre 5 e 15 ºC sem prejuízo para a qualidade fisiológica. Considerando-se o custo-benefício, as sementes podem ser armazenadas até 21 dias no interior do fruto em temperaturas de 5, 10 ou 15 ºC.

The aim of this work was describe the germination and evaluate the desiccation and reduction in desiccation-sensitivity of Hancornia speciosa Gomes seeds. Initially, we evaluated the germination characteristics and morphophysiological aspects of seedlings. The seeds in the first experiment were subjected to fast desiccation (activated silica gel) and slow desiccation (laboratory conditions) until the water content reached 40%, 30%, 20%, and 15% ± 2%. To reduce the desiccation sensitivity, in the second experiment, the seeds were soaked in polyethylene glycol (PEG) at potentials of -0.1 and -0.3 MPa for 120 h, with or without abscisic acid (ABA) (10-4µM), and subsequently subjected to fast desiccation until a water content of 15% was reached, based on the results from the first experiment. The seed vigor in both experiments was evaluated by the primary root protrusion tests, percentage of normal seedlings, germination speed index, length and mass (shoot, underground, and total). Seedlings of H. speciosa feature a stem-like xylopodium structure. The seeds were tolerant to water reduction up to 15% by fast desiccation and 30% by slow desiccation. Moreover, priming was not efficient in reducing the desiccation sensitivity of H. speciosa seeds.

O processo de salinização dos solos e das águas subterrâneas e superficiais é um dos mais importantes problemas de degradação ambiental. O niger é uma herbácea anual com potencial para a produção de biodiesel, cujo comportamento em condições salinas ainda é desconhecido. Dessa forma, objetivou-se avaliar a germinação de sementes e crescimento de plântulas de niger submetidos a diferentes sais e concentrações. Os tratamentos utilizados corresponderam a três sais: cloreto de potássio (KCl), cloreto de cálcio (CaCl2) e cloreto de sódio (NaCl), associados a quatro potenciais osmóticos (-0,3; -0,6; -0,9 e -1,2 MPa). As sementes de niger semeadas diretamente sobre substrato umedecido com água destilada constituíram o controle. O efeito da salinidade na germinação das sementes foi avaliado pela porcentagem, tempo médio e índice de velocidade. As plântulas foram analisadas quanto ao comprimento da parte aérea e raiz e massas fresca e seca de plântulas inteiras. O delineamento estatístico adotado foi o inteiramente casualizado, constituindo-se de um fatorial 3x5 (sais x potenciais osmóticos) com quatro repetições de 50 sementes cada. As sementes de niger são sensíveis a salinidade. A exposição ao NaCl, KCl e CaCl2 a partir do potencial osmótico de -0,3 MPa reduz o poder germinativo e o crescimento de plântulas. Os sais inibem a germinação de sementes do niger no potencial de -1,2 MPa.The soil, and subterranean and superficial water saltiness process is one of the most important environment degradation problems. Niger is an annual herbaceous with biodiesel production potential, which seeds behavior is unknown in saline conditions. In this way, this work aimed to evaluate the niger seeds germination and initial seedlings growth submitted to different salt solutions. The treatments used constituted of three types of salts: NaCl, KCl and CaCl2 at four osmotic potentials (-0.3; -0.6; -0.9 e -1.2 MPa). The niger seeds sowed directly on substrate water moistured constituted the control treatment. The saline effects on seeds germination were evaluated though germination percentage, germination medium time and germination speed index. The Niger seedlings were analyzed through the shoot and root length and fresh and dried mass seedlings. The statistic design used was entirely casualized, constituted by a factorial 3x5 (salts x osmotic potentials) with four repetitions of 50 seeds. The Niger seeds are salinity sensitive. Seeds exposition to NaCl, KCl and CaCl2 reduces the germinative ability and the seedlings growth at -0.3 MPa. The salt solutions inhibit the Niger seeds germination at -1.2 MPa.

Vários trabalhos têm sido conduzidos com diferentes substâncias para simular potenciais osmóticos negativos durante as primeiras fases da germinação, uma vez que, a absorção de água pelas sementes tem grande importância para iniciar o processo germinativo. Assim, objetivou-se avaliar o efeito de diferentes disponibilidades hídricas do substrato na qualidade fisiológica de sementes de canola com diferentes teores de água das sementes. Foram utilizadas sementes do híbrido HYOLA 433 com teores de água de 5,5, 7,1 e 11,0%, submetidas às seguintes disponibilidades hídricas no substrato simulados com soluções aquosas de polietilenoglicol (PEG 6000): 0,0; -0,1; -0,2; -0,3; -0,4 e -0,6 MPa. As sementes foram semeadas sobre duas folhas de papel tipo “germitest” em caixas tipo “gerbox” e mantidas a 25ºC e luz branca constante. O delineamento experimental utilizado foi o inteiramente casualizado em esquema fatorial 6 x 3 (disponibilidades hídricas do substrato e teores de água das sementes), com quatro repetições de 50 sementes cada, sendo os dados submetidos à análise de regressão. A redução da disponibilidade hídrica do substrato a partir de -0,3 MPa reduziu significativamente a germinação das sementes e o desempenho das plântulas de canola. Os teores de água avaliados não influenciaram a germinação das sementes. A redução da formação de plântulas normais e do crescimento de plântulas pode ser associada à diminuição das disponibilidades hídricas do substrato. As sementes de canola são sensíveis à redução do potencial hídrico abaixo de -0,2 MPa, que prejudica a germinação e o vigor das sementes

Objetivou-se com este estudo avaliar o potencial alelopático de extratos metanólicos de folhas, flores e raízes de capuchinha (Tropaeolum majus L.) sobre a germinação de sementes e o crescimento inicial de plântulas de picão-preto. O extrato metanólico com melhor potencial de inibição foi submetido a particionamento, resultando nas frações hexânica, clorofórmica, acetato de etila e hidrometanólica e posterior caracterização pelo espectro de absorção na região do infravermelho (IV). O efeito alelopático foi avaliado sobre as sementes de picão-preto, as quais foram distribuídas sobre papel germitest umedecido com 2mL dos extratos e mantidas em germinador do tipo B.O.D. regulado a temperatura de 25°C e luz branca constante, sendo que as sementes imersas diretamente em água constituíram o tratamento controle. A avaliação da qualidade da semente foi realizada pelos testes de germinação e vigor (primeira contagem e comprimento de raiz primária e de hipocótilo das plântulas), em delineamento inteiramente ao acaso. O potencial alelopático das folhas de capuchinha foi maior em relação às demais partes da planta sobre a germinação das sementes, comprimento de hipocótilo e de raiz das plântulas de picão-preto. Estes efeitos podem estar associados à presença de grupos químicos polares, pois à medida que se aumentou a polaridade dos solventes detectou-se maior efeito inibitório sobre a germinação e o crescimento inicial de plântulas de picão-preto.This research aimed to evaluate the metanolic extracts allelopathic potential from leaves, flowers and roots of capuchinha (Tropaeolum majus L.) on picão-preto seeds germination and initial seedling growth. The best inhibitor metanolic extract was fractioned, in hexanic, cloroformic, etil acetate and hidrometanolic fractions and it was characterized through absorption spectrum using mid-infrared. To evaluate the allelopathic effect of metanolic extracts and the fractions aftereffect; it was used picão-preto seeds distributed on 2ml extracts moistured paper maintained in B.O.D. germinator at 25°C and white constant light. Seeds sowed directly in water moistured germitest paper constituted the control. The allelopathic potential extracts were evaluated through the first count, final germination percentage, seedlings primary root and hypocotyl length (cm). The experiment was carried out DIC with three replications of 25 seeds. The capuchinha leaves showed allelopathic potential on picão-preto seeds germination, hypocotyl and seedlings primary root length. It was suggested that such effects are associated to the presence of polar chemical groups because the increase in the solvents polarity detected the major picão-preto germination and seedlings growth inhibition effect.

Este trabalho foi desenvolvido com o objetivo de avaliar procedimentos para condução dos testes de germinação e tetrazólio na avaliação da qualidade de sementes de Eugenia pleurantha. O teste de germinação foi realizado com areia e rolo de papel, sendo que as sementes foram mantidas em câmaras do tipo B.O.D.s nas temperaturas de 20ºC, 25ºC, 30ºC e 35ºC e temperatura alternada de 20°C/30°C com 10 horas de escuro para a temperatura mais baixa e 14 horas de luz para a temperatura mais elevada. Foram utilizadas oito repetições de 25 sementes para cada tratamento. Para o teste de tetrazólio foram utilizadas quatro repetições de 25 sementes submetidas às seguintes concentrações do sal de tetrazólio: 0,075%, 0,1% e 0,5%, durante 4, 8 e 12 horas, em BOD no escuro a 30°C. De acordo com os resultados encontrados, observa-se que a germinação das sementes foi favorecida pelo substrato areia sob temperatura de 30ºC. Para o teste de tetrazólio, os resultados mostraram que não houve diferenças significativas entre os tratamentos. Porém, com o uso da concentração de 0,1% de sal de tetrazólio durante 4 horas a 30ºC obteve-se uma coloração uniforme que permitiu a diferenciação entre tecidos sadios, em deterioração e tecidos mortos.

No processo de produção comercial de mudas de gérbera, a cor da flor é uma das principais características morfológicas de interesse agronômico, sendo uma característica importante em programas de melhoramento genético. A utilização de marcadores moleculares pode servir para direcionar cruzamentos, confirmar novos híbridos ou genótipos mutantes e identificar novos genótipos para fins comerciais. Nesse contexto, o objetivo deste trabalho foi analisar a divergência genética entre seis cultivares de Gerbera jamesonii ('Jaguar Yellow', 'Jaguar Cream', 'Jaguar Lemon', 'Jaguar Salmon Pastel', 'Jaguar Red', 'Jaguar Deep Rose'). A análise de divergência genética entre as cultivares de gérbera foi realizada utilizando-se 21 primers, os quais amplificaram 37 fragmentos polimórficos de DNA, que foram usados para estimar o coeficiente de Jaccard, o qual apresentou uma média de 0,38, variando de 0,28 a 0,56. A estrutura genética entre as cultivares foi estimada pelo UPGMA, revelando dois grupos distintos, a 38% de similaridade genética. A maior similaridade genética encontrada (56%) foi entre as cultivares 'Jaguar Yellow' e 'Jaguar Lemon'. Os resultados demonstram que a técnica RAPD oferece uma maneira rápida, relativamente barata e útil para a caracterização da divergência genética entre as diferentes cultivares de Gerbera jamesonii com relação à cor da flor.During the commercial production of gerbera seedlings, flower color is one of the main morphological aspects that have an agronomic interest and becoming an important feature in genetic breeding programs. The use of molecular markers may serve to direct crossings, new hybrids and mutants, besides confirm and identify new genotypes for commercial purposes. In that context, this work aimed to analyze the genetic divergence among six cultivars of Gerbera jamesonii ('Jaguar Yellow', 'Jaguar Cream', 'Jaguar Lemon', 'Jaguar Salmon Pastel', 'Jaguar Red', 'Jaguar Deep Rose'). The genetic divergence among cultivars of gerbera was carried out with 21 primers, which amplified 37 DNA polymorphic fragments, used to estimate the Jaccard index and presented an average of 0,38, ranging from 0,28 to 0,56. The genetic structure among cultivars was estimated by UPGMA and revealed two distinct groups, at 38% genetic similarity. The largest genetic similarity found (56%) was between cultivars 'Jaguar Yellow' and 'Jaguar Lemon'. The results showed that the RAPD is a fast, relatively inexpensive and useful technique for genetic divergence characterization between different cultivars of Gerbera jamesonii.

O teste de raios X tem se demonstrado uma técnica muito eficiente no controle de qualidade de sementes de espécies florestais, caracterizando fases do desenvolvimento, danos causados por secagem, beneficiamento e predação, entre outros. Objetivouse com este trabalho avaliar a qualidade física de sementes de cedro (Cedrela fissilis) pelo teste de raios X. Para isso, após o beneficiamento, as sementes de cedro foram expostas às seguintes condições de intensidade de radiação e tempos (25 Kv durante 60/30 segundos e 30 Kv durante 40 segundos) para determinar o padrão de raios X. De acordo com a anatomia visualizada pela imagem radiográfica, as sementes foram classificadas em Sementes Cheias, Sementes Com Danos, Sementes Mal Formadas e Sementes Vazias. Em seguida, foi realizado o teste de germinação em BOD, utilizando-se substrato de papel umedecido dentro de caixas para germinação, sob 25ºC e luz branca constante. Ao final de 20 dias, foram registrados os números de plântulas normais, anormais e sementes mortas. Pelos resultados obtidos, a intensidade de radiação de 25 Kv durante 60 segundos permitiu melhor visualização das características internas e os danos observados pela imagem radiográfica afetaram a germinação das sementes, reduzindo a qualidade do lote.The X-ray has showed to be a very efficient technique in the quality control of forest tree seeds, characterizing development phases, damages caused by dehydration, improvement and infestation, among others. The aim of this work was to evaluate the physical quality of cedar seeds (Cedrela fissilis) by X-ray test. For this, the cedar seeds were exposed at the following conditions of radiation intensity and times (25 Kv during 60/30 seconds and 30 Kv during 40 seconds) to determine the pattern of X-rays. According to the anatomy visualized by the radiographic image, seeds were classified in Full Seeds; Seeds With Damages, Deformed Seeds and Empty Seeds. Soon after, the seeds were submitted to the germination test, accomplished in BOD, using paper substratum damped in boxes for germination under 25ºC and white light. After 20 days, the numbers of normal, abnormal seedlings and died seeds were registered. From the results obtained the intensity of radiation of 25 Kv in the time of exposure of 60 seconds allowed clear visualization of the internal characteristics and damages observed. By the radiographic damage affected the seed germination reducing the plot quality.

Objetivou-se com este trabalho avaliar a atividade alelopática, antioxidante e o conteúdo de fenóis e flavonóides totais no extrato metanólico de quatro plantas medicinais: Hibiscus sabdariffa, Ocimum gratissimum, Palicourea crocea e Trichilia silvatica. Para isso, folhas, flores e caules de Trichilia silvatica, folhas de Palicourea crocea, cálice e folhas de Hibiscus sabdariffa e folhas de Ocimum gratissimum foram utilizados. A avaliação da atividade alelopática foi realizada pelo ensaio de germinação e crescimento inicial de sementes de alface (Lactuca sativa), com três repetições de 50 sementes em delineamento inteiramente casualizado. A atividade antioxidante foi determinada pelo método fotocolorimétrico in vitro do radical livre estável 2,2-difenil-1-picril-hidrazila (DPPH). O crescimento das plântulas de alface foi mais sensível à ação dos aleloquímicos presentes nos extratos metanólicos do que a germinação das sementes, e evidenciaram o efeito inibitório dos extratos metanólicos do cálice e folhas de H. sabdariffa e das folhas de O. gratissimum e P. crocea. Os extratos metanólicos obtidos das folhas, flores e casca de T. silvatica apresentaram maior capacidade de sequestrar radicais livres com valores de IC50 de 62,36; 19,4 18,38 µg mL-1, no ensaio com DPPH e maior teor de compostos fenólicos 520,80; 545,27 e 546,56 mg de ácido gálico g de amostra-1, respectivamente, avaliado pelo ensaio de Folin-Ciocalteau.

O niger (Guizotia abyssinica Cass.) é uma oleaginosa com potencial para a produção de biodiesel com poucas informações a respeito da qualidade fisiológica com que suas sementes são produzidas. Objetivou-se avaliar a germinação e o crescimento das plântulas de niger sob diferentes temperaturas e disponibilidades hídricas no substrato. O primeiro experimento foi conduzido em câmaras de germinação do tipo B.O.D. com temperaturas constantes (5, 10, 15, 20 e 25 °C) e uma temperatura alternada (20-30 °C). Para o segundo experimento as sementes foram semeadas sobre substrato de papel umedecido com soluções de Polietileno Glicol 6000 em diferentes potenciais osmóticos (0,0; -0,2; -0,4; -0,8 e -1,2 MPa) e mantidas em câmara de germinação do tipo B.O.D. na temperatura de 25°C. Avaliou-se a porcentagem, o índice de velocidade e o tempo médio de germinação, os comprimentos da parte aérea e da raiz e as massas fresca e seca das plântulas. As temperaturas de 20, 25 e 20-30 °C aumentaram a porcentagem e a velocidade de germinação das sementes de niger, que são sensíveis a temperaturas inferiores a 15 °C. A redução da disponibilidade hídrica do substrato a partir do potencial osmótico de -0,2 MPa prejudica a germinação e o crescimento de plântulas.

Eugenia pleurantha é uma árvore pertencente à família Myrtaceae, conhecida popularmente pelos nomes de cafezinho e pitanga-do-mato, e que tem ocorrência no sudeste e no sul do Brasil. É uma espécie propagada por sementes e o conhecimento sobre métodos de avaliação da qualidade de sementes é de fundamental importância para a utilização desta espécie em plantios de reposição e demais programas florestais em que pode ser empregada. Este trabalho foi desenvolvido com o objetivo de avaliar procedimentos para condução dos testes de germinação e tetrazólio na avaliação da qualidade de sementes de Eugenia pleurantha. Para o teste de germinação, as sementes foram mantidas em BODs sobre areia e rolo de papel, sob as temperaturas de 20°, 25°, 30° e 35° C com luz branca constante e temperatura alternada de 20/30° com 10 horas de escuro para a temperatura mais baixa e 14 horas de claro para a temperatura mais elevada. Foram utilizadas oito repetições de 25 sementes para cada tratamento. Para o teste de tetrazólio, as sementes foram embebidas em água destilada por 12 horas a 30° C; em seguida, os tegumentos foram removidos e as sementes foram seccionadas longitudinalmente. Foram utilizadas quatro repetições de 25 sementes submetidas às seguintes concentrações do sal de tetrazólio: 0,075%, 0,1% e 0,5%, durante 4, 8 e 12 horas, em BOD no escuro a 30° C. A germinação das sementes foi favorecida pelo substrato areia sob temperatura de 30° C e, para o teste de tetrazólio, os resultados mostraram que não houve diferenças significativas entre os tratamentos. Porém, com o uso da concentração de 0,1% de sal de tetrazólio durante 4 horas a 30° C, obteve-se uma coloração uniforme que permitiu a diferenciação entre tecidos sadios, em deterioração e tecidos mortos. Assim, este pode ser um procedimento eficiente para a avaliação da qualidade fisiológica das sementes de Eugenia pleurantha.

A cagaiteira (Eugenia dysenterica DC.), também conhecida como cagaita devido às suas propriedades laxativas, é uma árvore frutífera nativa natural do Cerrado. Possui altura mediana, atingindo de 4 a 10 m, tronco tortuoso e cilíndrico com 20 a 40 cm de diâmetro e uma casca suberosa e fendada bem característica. A cagaiteira apresenta um grande potencial como planta frutífera, destaca-se das demais por apresentar uma produtividade elevada, chegando a 2.000 frutos por árvore. Sua polpa de sabor doce levemente ácido é excelente para a produção de sorvetes, geléias, doces e licores (Almeida et al., 1987) e os frutos, ainda imaturos, podem ser utilizados como forragem para o gado (Donadio et al., 2002; Ribeiro et al., 1986). De sua polpa também são obtidos vinagre e álcool (Corrêa, 1984). Apresenta madeira pesada de boa qualidade. Suas folhas apresentam propriedades antidiarréica e utilizadas para problemas do coração. Costa et al. (2000) verificaram elevada atividade antifúngica no óleo hidrolisado de suas folhas. Devido ao seu florescimento abundante é considerada ainda como paisagística (Ribeiro et al., 1994; Gavilanes et al., 1991) e melífera (Brandão & Ferreira, 1991). Quanto a sua propagação por via sexuada, apresenta elevada variabilidade e certo grau de dormência (Rizzini, 1971). Andrade et al. (2003) verificaram que suas sementes apresentam recalcitrância, onde perdiam completamente a viabilidade quando eram dessecadas abaixo de 18 % de teor de água. Técnicas de propagação vegetativa massal, como o cultivo in vitro, podem superar problemas de dormência e recalcitrância da espécie, produzindo mudas uniformes de qualidade e em larga escala. Dentre estas técnicas temos a propagação a partir da indução de calos (calogênese). Por meio da calogênese, pode-se obter a propagação massal de espécies que apresentem dificuldade de multiplicação por via sexuada, podendo ser empregada também na produção de metabólitos secundários, sendo ainda essencial em métodos de transformação genética de plantas. Calos são definidos como aglomerados de células parenquimáticas não organizadas, irregularmente diferenciadas, que se multiplicam desordenadamente e se desenvolvem em resposta a injúrias químicas ou físicas. No entanto, os calos podem se diferenciar em tecidos e órgãos (Torres et al., 1998). O presente trabalho teve por objetivo a indução de calos de explantes foliares de cagaiteira em meio líquido utilizando BAP e 2,4-D.

A palma forrageira (Opuntia ficus) é o alimento mais utilizado pelos produtores na maior Bacia Leiteira do Nordeste em Batalha-Alagoas, principalmente na época do verão. É o único volumoso que mantém seu valor nutritivo mesmo sem parar de crescer (Melo, 2005). Somente em Alagoas, existem mais de 90 mil hectares de palma forrageira miúda. Estima-se existir, no Nordeste brasileiro, cerca de 400 mil hectares cultivados, constituindo-se numa das principais forrageiras na época seca (Santos et al., 1997). Atualmente, este vegetal se presta às mais diversas utilidades, por ser amplamente difundido, de fácil plantio e altamente resistente à seca. A palma forrageira é uma das principais culturas ornamentais da região Nordeste do Brasil e é principalmente utilizada na indústria alimentícia, na indústria farmacêutica e de cosméticos, além de ser empregada no setor agronômico entre outros (Sebrae, 2001). A cultura de tecidos é um meio de desenvolver células ou tecidos vegetais sob condições controladas. Pode ser definida como a cultura de células vegetais isoladas, de um grupo de células, tecidos ou órgãos em ambiente artificial, sob condições assépticas. Nesse ambiente, as células, tecidos e órgãos se multiplicam e continuam a crescer de modo não organizado ou se regeneram numa planta inteira (Da Silva, 2007). A contaminação dos explantes é um dos principais problemas do cultivo in vitro de espécies lenhosas (Pierik, 1990). Os níveis de contaminação tendem a ser maiores quando as plantas matrizes usadas como fonte de explantes são provenientes do campo. No entanto, mesmo as plantas submetidas ao rigoroso controle fitossanitário e mantidas em viveiro protegido ou casa de vegetação são fontes potenciais de microorganismos, que podem tornar-se limitantes aos procedimentos de cultivo in vitro (Medeiros, 1999) Este trabalho teve por objetivo avaliar o efeito de diferentes tempos de imersão em solução de hipoclorito de sódio e de bicloreto de mercúrio na desinfestação e sobrevivência de explantes de palma forrageira.

Abstract: This study evaluated the physiological potential of soybean seeds harvested during two seasons, on different maturation stages and subjected to different drying temperatures. The seeds were harvested at the maturations stages R7, R7 + 2, R7 + 3, R7 + 5, R7 + 6, R7 + 7, R7 + 10 and R7 + 12 days (55, 50, 45, 40, 35, 30, 25, and 20% of moisture content). For each maturation stage, seeds were divided into three samples: one sample was used to directly evaluate the physiological potential, and the others were dried at 40 °C and 50 °C, until reaching the moisture content of 11.5%. The physiological potential was evaluated through germination test, first germination count of germination, accelerated aging, modified cold, electrical conductivity and seedling emergence. The maximum physiological potential of seeds is achieved at the moisture content of 55%, the point that the dry matter is maximum. The seeds became tolerant to artificial drying approximately at the stage R7 + 7 days (30% of moisture content). Germination and vigor of the soybean seeds reduce as the drying temperature is increased from 40 °C to 50 °C, and this effect is enhanced when the seeds show moisture contents above 30%.

Abstract:Niger is a promising oilseed species for biodiesel production but there is no much information about the physiological potential of its seeds. Thus, the aim was to adapt the methodologies of accelerated aging test on six lots of niger seeds. The test was carried out by traditional and with saturated salt solution (20 and 40 g NaCl.100 mL-1) methods at 41 and 45 °C for 24, 48, 72 and 96 hours. After the decay period, the seeds were submitted to the germination test, proceeding to an evaluation on the seventh day after sowing, counting the normal seedlings percentage. A completely randomized design with four replications of 50 seeds was used and the means were compared by Tukey's test. The accelerated aging test was correlated with seedling emergence and provided lots classification in at least two levels of vigor. For the accelerated aging test, the method with 20 g NaCl.100 mL-1 at 41 °C for 24 hours is recommend. The traditional method is not suitable because it provides water content variation between samples above what is tolerable.

Abstract: Drying of seeds reduces their moisture content to levels appropriate for storage. However, care in the temperatures applied in the process is necessary to avoid damage to the seeds that are dried. The aim of this study was to evaluate the effect of different drying temperatures and storage on the growth of soybean seedlings. Harvested with a moisture content of approximately 23% (w.b.), the seeds were dried at different temperatures (40, 50, 60, 70, and 80 °C) until reaching moisture content of 12.5 ± 0.7% (w.b.), and they were subsequently stored for 180 days in an environment without climate control. A germination test and evaluations of seedling performance were carried out every 45 days, determining the full length of the seedling and hypocotyl and root lengths, along with their respective dry matter weights. The results showed that: a) the increase in the temperature of drying air affects the physiological quality of soybean seeds, and this effect is accentuated over time, especially on root length; and b) the air temperature of 40 °C can be recommended for drying of soybean seeds in association with the storage time of 180 days under storage conditions without climate control.

Hancornia speciosa is a native tree species of the Brazilian Cerrado whose seeds are desiccation sensitive. In this study, we aimed to evaluate drying and osmotic conditioning in H. speciosa seeds. We used fresh seeds with 48% moisture content, which were slowly dried until they attained contents of 20%, 15%, 10% and 5%. To evaluate osmotic conditioning, the seeds were imbibed in 12 mL osmotic solutions at 0.0; -0.2; -0.4 and -0.6 MPa for two days. After that, they were dehydrated until their original moisture content. The experiments were carried out in a completely randomized design with four repetitions with 50 seeds each. Reduction in moisture content from 20% to 5% decreased the physiological potential of seeds. H. speciosa seeds do not require osmotic priming with PEG solutions, because imbibition of seeds in osmotic solutions of up to -0.6 MPa results in reduction of germination rate and seedling length.

Abstract: Dehydration is a necessary procedure prior to exposing seeds to long term storage, but this is associated with metabolism-linked injury mediated by cell injury. In order to assess cellular alterations during re-establishment of desiccation tolerance (DT) in C. fissilis germinated seeds and their relation to DNA damage, we verified the occurrence of DNA fragmentation through the TUNEL test and its evidence through the cytological analyses. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moisture content) followed by rehydration. The moisture content changes during the reestablishment of the desiccation tolerance was accomplished. (DT)TdT-dUPT terminal nick-end labeling (TUNEL) was used to assess rates of cell death. TUNEL staining was performed using Click-iT-TUNEL Alexa Flour imaging assay. The TUNEL test showed a consistent DNA fragmentation in the 2 and 5 mm long radicles. Moreover, nuclear and chromosomal alterations were observed in the 5 mm meristematic root cell cycle, contributing to the identification of diagnostic markers of cell death.

Abstract: Safflower seeds exhibit dormancy soon after dispersion from the mother plant, making it impossible to sow newly harvested seeds. Thus, the aim of the present study was to evaluate breaking the dormancy of safflower seeds during storage associated with the use of different periods of cold stratification. Seeds with a moisture content of 7.2% were stored for 0, 60, 120, 180, and 240 days, and for each storage period, they were stratified for 0 (control), 1, 2, 3, 4, 5, 6, and 7 days at 10 °C in the dark. After stratification, seeds were subjected to the germination test and evaluated for percentage of root protrusion, germination speed index, and percentages of first count and final count of normal seedlings. Safflower seeds gradually overcome dormancy during storage for 240 days in a non-controlled environment. Storage associated with periods of cold stratification for 5 to 7 days lead to an increase in germination and are effective in breaking the physiological dormancy of safflower seeds.