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3. Metabolic Reprogramming of Klebsiella pneumoniaeExposed to Serum and Its Potential Implications in Host Immune System Evasion and Resistance

Author

Moraes, Amanda Naiara Silva, Tatara, Juliana Miranda, da Rosa, Rafael Lopes, Siqueira, Franciele Maboni, Domingues, Guilherme, Berger, Markus, Guimarães, Jorge Almeida, Barth, Afonso Luís, Barth, Patricia Orlandi, Yates, John R., Beys-da-Silva, Walter Orlando, and Santi, Lucélia

Abstract

The aim of this study was to identify, using proteomics, the molecular alterations caused by human serum exposure to Klebsiella pneumoniaeACH2. The analysis was performed under two different conditions, native serum from healthy donors and heat-inactivated serum (to inactivate the complement system), and at two different times, after 1 and 4 h of serum exposure. More than 1,000 bacterial proteins were identified at each time point. Enterobactin, a siderophore involved in iron uptake, and proteins involved in translation were upregulated at 1 h, while the chaperone ProQ and the glyoxylate cycle were identified after 4 h. Enzymes involved in the stress response were downregulated, and the SOD activity was validated using an enzymatic assay. In addition, an intricate metabolic adaptation was observed, with pyruvate and thiamine possibly involved in survival and virulence in the first hour of serum exposure. The addition of exogenous thiamine contributes to bacterial growth in human serum, corroborating this result. During 4 h of serum exposure, the glyoxylate cycle (GC) probably plays a central role, and the addition of exogenous succinate suppresses the GC, inducing a decrease in serum resistance. Therefore, serum exposure causes important changes in iron acquisition, the expression of virulence factors, and metabolic reprogramming, which could contribute to bacterial serum resistance.

Published
2024
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5. Contributors

Author

Abram, Quinn H., primary, Addas-Carvalho, Marcelo, additional, Alpuche-Lazcano, Sergio P., additional, Alves, Maria João, additional, Alves-Leon, Soniza Vieira, additional, Amaro, Fátima, additional, Araújo, Josélio Maria Galvão de, additional, Araujo-Pereira, Mariana, additional, Aspahan, Maíra Cardoso, additional, Barbosa, Maria Helena de M., additional, Barnard, Trisha R., additional, Beck, Cécile, additional, Benites, Bruno Deltreggia, additional, Beys-da-Silva, Walter Orlando, additional, Bloom, Marshall E., additional, Boivin, Guy, additional, Brault, Aaron C., additional, Cabral-Marques, Otavio, additional, de Caires Junior, Luiz Carlos, additional, Cardoso, Cynthia Chester, additional, Carrillo-Hernández, Marlen Yelitza, additional, Chimelli, Leila, additional, Christo, Paulo Pereira, additional, Chu, Dinh-Toi, additional, Coombs, Kevin M., additional, Costa, Fabio T.M., additional, Costa Monteiro, Lucia Maria, additional, Cristina, Juan, additional, Cunha, Marielton Dos Passos, additional, da Silva Augusto, Lia Giraldo, additional, Desprès, Philippe, additional, Diaz, Adrián, additional, Diderichsen, Finn, additional, Duarte, Alberto José da Silva, additional, Dumarest, Marine, additional, El Khawanky, Nadia, additional, Fernandes, José Veríssimo, additional, Fonseca, Simone G., additional, Fontes-Dantas, Fabrícia Lima, additional, Gadea, Gilles, additional, Gallo, Luciana Guerra, additional, Gatignol, Anne, additional, Gaytán, David Alejandro Cabrera, additional, Gonzalez, Gaelle, additional, Goulart, Ernesto, additional, Gregorio, Ernesto R., additional, Guedes, Paulo Marcos Matta, additional, Hamel, Rodolphe, additional, Hofer, Cristina Barroso, additional, Ismail, Amni Adilah, additional, Jääskeläinen, Anne J., additional, Jen, Soe Hui, additional, Judice, Carla, additional, Kaid, Carolini, additional, Kobayashi, Jun, additional, Lecollinet, Sylvie, additional, Leyser, Marcio, additional, de Magalhães-Barbosa, Maria Clara, additional, Manikam, Rishya, additional, Marques, Fernanda J.P., additional, Martínez-Gutiérrez, Marlen, additional, Martins, Felipe, additional, de Matos, António Pedro Alves, additional, McDonald, Erin M., additional, Meltzer, Eyal, additional, Mendoza, Teresita Rojas, additional, Mitsugi, Thiago, additional, Mlera, Luwanika, additional, Muñiz, Concepción Grajales, additional, Nakaya, Helder I., additional, Nascimento, Andrezza, additional, Nascimento, Gilmara Lima, additional, Nascimento, Manuela Sales Lima, additional, Nascimento, Osvaldo J.M., additional, Nguyen, Tiep Tien, additional, Nieto, Lumumba Arriaga, additional, de Oliveira, Maria Regina Fernandes, additional, Owczarek, Katarzyna, additional, Parás, Alfonso Vallejos, additional, Patel, Vinood B., additional, Peixoto, Henry Maia, additional, Pereira-Gómez, Marianoel, additional, Pfrimer, Irmtraut Araci H., additional, Phumee, Atchara, additional, Piret, Jocelyne, additional, Prata-Barbosa, Arnaldo, additional, Preedy, Victor R., additional, Pyrć, Krzysztof, additional, Rajendram, Rajkumar, additional, Raju, Chandramathi Samudi, additional, Rossi, Átila Duque, additional, Rueda-Lopes, Fernanda Cristina, additional, Ruiz-Saenz, Julian, additional, Sagan, Selena M., additional, Salmeron, Amanda Costa Ayres, additional, Sanabani, Sabri Saeed, additional, Santi, Lucélia, additional, Santos, Wilo Victor dos, additional, Schuch, Viviane, additional, Sekaran, Shamala Devi, additional, Sherwood, Matt, additional, Shrivastava, Prateek Saurabh, additional, Shrivastava, Saurabh RamBihariLal, additional, Siriyasatien, Padet, additional, Son, Nguyen Thai, additional, Tatara, Juliana Miranda, additional, Ten Caten, Felipe, additional, Tho, Ho Huu, additional, Viranaicken, Wildriss, additional, Vivacqua, Daniela Pires Ferreira, additional, Xu, Dan, additional, Xu, Zhiheng, additional, Zatz, Mayana, additional, and Zé-Zé, Libia, additional

Published
2021
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6. SARSCOVIDB : a new platform for the analysis of the molecular impact of SARS-CoV-2 viral infection

Author

Rosa, Rafael Lopes da, Yang, Tung Sheng, Cagnini, Emanuela Fernanda Tureta, Oliveira, Laura Rascovetzki Saciloto de, Moraes, Amanda Naiara Silva, Tatara, Juliana Miranda, Costa, Renata Pereira, Borges, Júlia Spier, Alves, Camila Innocente, Oliveira, Markus Berger, Guimaraes, Jorge Almeida, Santi, Lucélia, and Silva, Walter Orlando Beys da

Subjects
Doenças transmissíveis, Proteômica, Infecções por coronavirus, Interfaces, Genetics, Infecções por coronavirus [Base de dados], Infectious diseases, Biological databases
Abstract

The COVID-19 pandemic caused by the new coronavirus (SARS-CoV-2) has become a global emergency issue for public health. This threat has led to an acceleration in related research and, consequently, an unprecedented volume of clinical and experimental data that include changes in gene expression resulting from infection. The SARS-CoV-2 infection database (SARSCOVIDB: https://sarscovidb.org/) was created to mitigate the dificulties related to this scenario. The SARSCOVIDB is an online platform that aims to integrate all differential gene expression data, at messenger RNA and protein levels, helping to speed up analysis and research on the molecular impact of COVID-19. The database can be searched from different experimental perspectives and presents all related information from published data, such as viral strains, hosts, methodological approaches (proteomics or transcriptomics), genes/proteins, and samples (clinical or experimental). All information was taken from 24 articles related to analyses of differential gene expression out of 5,554 COVID-19/SARS-CoV-2-related articles published so far. The database features 12,535 genes whose expression has been identified as altered due to SARS-CoV-2 infection. Thus, the SARSCOVIDB is a new resource to support the health workers and the scientific community in understanding the pathogenesis and molecular impact caused by SARS-CoV-2.

Published
2021

7. SARSCOVIDB—A New Platform for the Analysis of the Molecular Impact of SARS-CoV-2 Viral Infection

Author

da Rosa, Rafael Lopes, primary, Yang, Tung Sheng, additional, Tureta, Emanuela Fernanda, additional, de Oliveira, Laura Rascovetzki Saciloto, additional, Moraes, Amanda Naiara Silva, additional, Tatara, Juliana Miranda, additional, Costa, Renata Pereira, additional, Borges, Júlia Spier, additional, Alves, Camila Innocente, additional, Berger, Markus, additional, Guimarães, Jorge Almeida, additional, Santi, Lucélia, additional, and Beys-da-Silva, Walter Orlando, additional

Published
2021
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9. Giant viruses inhibit superinfection by downregulating phagocytosis in Acanthamoeba.

Author

Aquino, Isabella L. M., Sousa Reis, Erik, Almeida Mattos Moreira, Rafaella Oliveira, Colquehuanca Arias, Nídia Esther, Barcelos, Matheus Gomes, Fulgêncio Queiroz, Victória, Arifa, Raquel Duque do Nascimento, Barbosa Lucas, Larissa Mendes, Tatara, Juliana Miranda, Souza, Daniele G., Costa, Adriana, Rosa, Luiz, Almeida, Gabriel M. F., Kroon, Erna Geessien, and Abrahão, Jônatas S.

Subjects
*HOST-virus relationships, *SUPERINFECTION, *ACANTHAMOEBA, *PHAGOCYTOSIS, *AMOEBA
Abstract

In the context of the virosphere, viral particles can compete for host cells. In this scenario, some viruses block the entry of exogenous virions upon infecting a cell, a phenomenon known as superinfection inhibition. The molecular mechanisms associated with superinfection inhibition vary depending on the viral species and the host, but generally, blocking superinfection ensures the genetic supremacy of the virus's progeny that first infects the cell. Giant amoeba-infecting viruses have attracted the scientific community's attention due to the complexity of their particles and genomes. However, there are no studies on the occurrence of superinfection and its inhibition induced by giant viruses. This study shows that mimivirus, moumouvirus, and megavirus, exhibit different strategies related to the infection of Acanthamoeba. For the first time, we have reported that mimivirus and moumouvirus induce superinfection inhibition in amoebas. Interestingly, megaviruses do not exhibit this ability, allowing continuous entry of exogenous virions into infected amoebas. Our investigation into the mechanisms behind superinfection blockage reveals that mimivirus and moumouvirus inhibit amoebic phagocytosis, leading to significant changes in the morphology and activity of the host cells. In contrast, megavirus-infected amoebas continue incorporating newly formed virions, negatively affecting the available viral progeny. This effect, however, is reversible with chemical inhibition of phagocytosis. This work contributes to the understanding of superinfection and its inhibition in mimivirus, moumouvirus, and megavirus, demonstrating that despite their evolutionary relatedness, these viruses exhibit profound differences in their interactions with their hosts. [ABSTRACT FROM AUTHOR]

Published
2024
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