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Background: Obesity may induce oxidative stress, causing oxidative damage of DNA. We examined associations between decreasing serum and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels and weight loss in morbidly obese patients before and 6 months after laparoscopic adjustable gastric banding (LAGB). Methods: We compared patients who had surgery for morbid obesity with healthy, nonobese controls. Urine and fasting blood samples were collected once from the controls and from the morbidly obese patients before and 6 months after the LAGB. The serum and urinary 8-OHdG levels were evaluated in these groups using an enzyme-linked immunosorbent assay kit. Results: We included 20 patients who had surgery for morbid obesity (8 men, 12 women, mean body mass index [BMI] 46.82 [+ or -] 4.47) and 20 healthy, nonobese people (10 men, 10 women, mean BMI 22.52 [+ or -] 2.08) in our study. There was no significant difference in serum 8-OHdG levels between the groups, whereas urinary 8-OHdG levels were significantly higher in morbidly obese patients than in controls. Weight, BMI and serum and urinary 8-OHdG levels were significantly decreased in morbidly obese patients 6 months after LAGB. Conclusion: The LAGB provides efficient weight loss in patients with morbid obesity. The systemic oxidative DNA damage was increased by the morbid obesity, but this increase was not related to weight gain, and it was more evident in serum than urine samples. After LAGB for morbid obesity, the oxidative DNA damage declined both in serum and urine. Contexte: L'obesite peut provoquer stress oxydatif qui endommage l'ADN. Nous avons analyse les liens entre une baisse des taux de 8-OHdG (8-hydroxy-2'-desoxyguanosine) seriques et urinaires et la perte de poids chez des patients atteints d'obesite morbide avant, puis 6 mois apres la pose d'un anneau gastrique ajustable par laparoscopie (AGAL). Methodes: Nous avons compare des patients qui ont subi cette chirurgie pour un probleme d'obesite morbide a des temoins non obeses en bonne sante. Nous avons preleve des echantillons d'urine et de sang a jeun chez les temoins 1 fois et chez les patients atteints d'obesite morbide, avant, puis 6 mois apres l'intervention pour AGAL. Les taux de 8-OHdG seriques et urinaires ont ete mesures dans les 2 groupes a l'aide d'une trousse de test ELISA (enzyme-linked immunosorbent assay). Resultats: Notre etude a inclus 20 patients soumis a la chirurgie pour obesite morbide (8 hommes, 12 femmes; indice de masse corporelle [IMC] moyen 46,82 [+ or -] 4,47) et 20 temoins non obeses en bonne sante (10 hommes, 10 femmes; IMC moyen 22,52 [+ or -] 2,08). Nous n'avons note aucune difference significative des taux de 8-OHdG seriques entre les 2 groupes, mais les taux de 8-OHdG urinaires etaient significativement plus eleves chez les patients souffrant d'obesite morbide que chez les temoins. Le poids, l'IMC et les taux de 8-OHdG seriques et urinaires avaient significativement diminue chez les patients atteints d'obesite morbide 6 mois apres l'intervention pour AGAL. Conclusion: L'AGAL est une technique efficace de perte de poids chez les patients souffrant d'obesite morbide. L'atteinte oxydative systemique de l'ADN etait exacerbee par l'obesite morbide, mais cette hausse n'etait pas reliee au gain ponderal, et elle etait plus evidente dans les echantillons seriques que dans les echantillons urinaires. Apres la pose d'un AGAL pour obesite morbide, l'atteinte oxydative de l'ADN a diminue dans le serum et dans l'urine., Reactive oxygen species (ROS) are produced by living organisms as a result of normal cellular metabolism. At low to moderate concentrations, they function in physiologic cell processes, but at high [...]

Background: The aim of this study was to examine the relationship between weight loss and resistin, apelin, chemerin, and visfatin after laparoscopic adjustable gastric banding (LAGB). Methods: The study group consisted of 19 patients who were operated on for morbid obesity (BMI: 48.7 ± 6.6 kg/m2), and 22 healthy, normal-weight (BMI: 22.9 ± 2.5 kg/m2) subjects formed the control group. We obtained blood samples from the study subjects at three different times: before undergoing surgery and at one month and 6 months after surgery. Blood was obtained once from the control group. Results: Significant weight loss was achieved at one and 6 months after surgery. Plasma levels of apelin, resistin, chemerin, and visfatin were higher in morbidly obese patients compared with the control group. Obesity-related peptides decreased one month and 6 months after surgery. Conclusions: Elevated plasma resistin, apelin, chemerin, and visfatin levels in morbidly obese patients are gradually reduced after weight loss. According to these findings, LAGB surgery is found to be an important and efficient means for morbidly obese patients both to lose weight and to develop a better metabolic risk profile in a short time period. [ABSTRACT FROM AUTHOR]