42 results on '"Tarnawska, M."'
Search Results
2. LP-54: Ascorbic acid and graphene oxide exposure in the model organism Acheta domesticus can change the reproduction potential
- Author
-
Flasz, B., primary, Tarnawska, M., additional, Nowak, N., additional, Kędziorski, A., additional, and Augustyniak, M., additional
- Published
- 2023
- Full Text
- View/download PDF
3. Micro-PIXE studies of elemental distribution in sap-feeding insects associated with Ni hyperaccumulator, Berkheya coddii
- Author
-
Migula, P., Przybyłowicz, W. J., Mesjasz-Przybyłowicz, J., Augustyniak, M., Nakonieczny, M., Głowacka, E., and Tarnawska, M.
- Published
- 2007
4. EURObservational Research Programme: the Chronic Ischaemic Cardiovascular Disease Registry: Pilot phase (CICD-PILOT)
- Author
-
Komajda, Michel, Weidinger, Franz, Kerneis, Mathieu, Cosentino, Francesco, Cremonesi, Alberto, Ferrari, Roberto, Kownator, Serge, Steg, Philippe Gabriel, Tavazzi, Luigi, Valgimigli, Marco, Szwed, Hanna, Majda, Wojciech, Olivari, Zoran, Van Belle, Eric, Shlyakhto, Evgeny Vladimirovich, Mintale, Iveta, Slapikas, Rimvydas, Rittger, Harald, Mendes, Miguel, Tsioufis, Constantinos, Balanescu, Serban, Laroche, Cécile, Maggioni, Aldo Pietro, Komajda, Michel, Weidinger, Franz, Cosentino, Francesco, Cremonesi, Alberto, Ferrari, Roberto, Kownator, Serge, Maggioni, Aldo P., Steg, Gabriel, Tavazzi, Luigi, Valgimigli, Marco, Balanescu, Serban, Mendes, Miguel, Mintale, Iveta, Olivari, Zoran, Rittger, Harald, Shlyakhto, Evgeny V., Slapikas, Rimvydas, Szwed, Hanna, Van Belle, Éric, Laroche, Cécile, McNeill, Patti-Ann, Ferreira, Thierry, Vochelet, F., Tavildari, A., Silvestri, M., Maillard, L., Sevilla, J., Malaquin, D., Leborgne, L., Fournier, A., Jarry, G., Teiger, E., Marchant, B., Van Belle, E., Criquioche, A., Dupouy, P., Madiot, H., Lafitte, B., Belle, L., Cayla, G., Abouth-Benamara, S., Messas, E., Delarche, N., Bouvier, E., Couleru, J., Paparoni, F., Marchant, B., Bedossa, M., Lepage, Q., Le Bouquin, L., Auffret, V., Leurent, G., Boulmier, D., Le Breton, H., Vidal, C., Touet, M., Tron, C., Hemmerling, S., Flugel, P.-C., Beschorner, U., Noory, E., Strubin, J., Macharzina, R., Lindemann, C., Specht, T., Brantner, R., Zeller, T., Schwarzwalder, U., Hirschmann, S., Schonhardt, S., Slimack-Braun, S., Link, S., Hauk, M., Welslau, M., Henning, A., Menz, C., Buciuceanu, V., Rastan, A., Bschorr, R., Schmitt, C., Maas, C., Jacques, B., Bohme, T., Burgelin, K., Hoffmann, M., Pirzer, R., Brune, M., Braun, M., Stolte, D., Dietrich, A., Singh, A., Rittger, H., Schibgilla, V., Hopf, S., Fouridis, P., Fabiani, R., Jakob, A., Garlichs, C., Trautvetter, J., Schineis, N., Brugger, A., Bojanic, D., Matschke, C., Schmidt, A., Scheinert, D., Scheinert, S., Banning-Eichenseer, U., Stauffer, A., Straube, F., Hoffmann, E., Antoni, D., Rieber, J., Tomelden, J., Deichstetter, M., Landwehr, P., Reif, S., Kallikazaros, I., Tsioufis, C., Korkovili, M., Stamatelopoulos, K., Tsoumani, Z., Loizos, S., Kanakakis, I., Kampouridis, N., Simeonidis, D., Bougas, T., Chasapi, A., Alexopoulos, D., Xanthopoulou, I., Vogiatzi, X., Stavrou, K., Bampouri, T., Sicuro, M., Amato, G., Pisano, F., Casolati, D., Bare, C., Grotti, S., Angioli, P., Bolognese, L., Ducci, K., Porto, I., Falsini, G., Liisto, F., Manzi, R.C., Langiu, M., Lai, C., Lodolini, V., Biscaglia, S., Ferrari, R., Occhilupo, P., Tebaldi, M., Minarelli, M., Fileti, L., Campo, G., Sbarzaglia, P., Bonilla, N., Schiavina, G., dʼAlessandro, G., Borghesi, M., Cremonesi, A., Rota, I., Ghigliotti, G., Davi, F., Beccaria, F., Brunelli, C., Mussardo, M., Cisotta, F., Spagnolo, B., Liso, A., Dal Corso, L., Favretto, G., Borsatto, F., Benedetto, M., Cirrincione, G., Gandolfo, C., Caruso, M., Stabile, A., Lanteri, S., Riina, M., Lunetto, M.L., Vadala, G., Micari, A., Rossi, E., Griksteite, E., Cavallini, C., Longhi, S., Pasqualini, M., Negrelli, M., Pacchioni, E., Brunazzi, M.C., Marcomin, C., Neri, R., Cassin, M., Vendrametto, F., Macor, F., Nicolosi, G., Pavan, D., Piasentin, C., Roman-Pognuz, A., Taccheri, T., Calcagno, S., Pagliaro, M., Mancone, M., Fedele, F., Cinque, A., Armato, A., Tarducci, R., Della Bona, R., Brandini, R., Rossi, P., Fronticelli, M., Casavecchia, M., Olivari, Z., Calzolari, D., Daniotti, A., Balcere, K., Stirna, V., Libins, A., Zabunova, M., Silina, E., Ozola, G., Rancane, G., Babarskiene, R., Viezelis, M., Petrauskaite, J., Rumbinaite, E., Stankala, S., Juszczyk, Z., Karwowska Polecka, W., Oleksza, A., Bialek, P., Klimczuk, A., Poplawski, A., Aksiucik, A., Musial, W., Swiecki, P., Marcinkiewicz-Siemion, M., Ptaszynska-Kopczynska, K., Prokop, J., Kubica, J., Janiszewska, E., Kopczynska, A., Tarnawska, M., Gruchala, M., Pajkowski, M., Raczak, G., Wojtowicz, D., Strozyk, A., Miekus, P., Szyman, M., Glaza, M., Roszko-Grycner, E., Szpajer, M., Wroblewska, M., Zadrozny, J., Muller, H., Puzio, E., Lesinski, D., Borowski, B., Kowalska, A., Wojtyniak, I., Krzewinska, J., Borej, G., Czaja, P., Janion, M., Zandecki, L., Kuczerowska, R., Bogacki, P., Kafara, M., Rola, A., Podolec, P., Waligora, M., Brozda, M., Skrzynska, M., Glowa, B., Gawor, Z., Dejak, P., Brylka, A., Banasiak, M., Simiera, M., Krecki, R., Ojrzanowski, M., Jankowski, L., Kupczynska, K., Kasprzak, J.D., Zapolski, T., Zarczuk, R., Lukasik, D., Wysokinski, A., Zalewska-Nowak, G., Tarnolicki, M., Major, M., Gorny, J., Krzyzanowski, W., Muzyk-Osikowicz, M., Boltryk, K., Grajek, S., Maczynski, M., Lesiak, M., Komosa, A., Drewnicki, A., Wolniewicz, L., Komorowska, E., Gmyrek, N., Kowalik, M., Kostka, M., Kaminski, L., Mikolowicz-Mosiadz, A., Mazur, R., Kosztowniak, M., Pajaczkowski, K., Duda, K., Kosno-Zak, J., Dworak-Podlewska, E., Burchard, E., Wrzosek, B., Gurba, S., Wozniak, P., Dabek, M., Kuzniar, J., Lyczywek, M., Szubielski, M., Gajewski, M., Wasiak, D., Oscik-Lukasiewicz, M., Kawka-Urbanek, T., Diks, F., Przywoska-Para, B., Drazkowicz-Gozdzik, B., Kornacewicz-Jach, Z., Kula, L., Goracy, J., Chlasta, J., Tomaniak, M., Opolski, G., Serafin, A., Pietrasik, A., Kosek, M., Jastrzebski, J., Witkowski, A., Pruszczyk, P., Roik, M., Kostrubiec, M., Irzyk, K., Wretowski, D., Labyk, A., Budaj, A., Maciejewski, P., Szwed, H., Majda, W., Chojecka, M., Mosur, M., Gajer-Blaszczyk, K., Bociaga, Z., Loboz-Rudnicka, M., Loboz-Grudzien, K., Jaroch, J., Rzyczkowska, B., Chelstowski, W., Lewandowska, A., Darocha, A., Skowron, W., Polonski, L., Maciol-Skurk, K., Madeira, S., Mendes, M., Brito, J., Santos, M., Leite, L., Vicente, J., Calisto, J., Faria, H., Jorge, E., Mendes, A., Santos, R., Pinto, P., Guedes, H., Placido, R., Correia, M.J., Cabrita, I., Rodrigues, C., Nunes Diogo, A., Magalhaes, A., Canas da Silva, A., Selas, M., Portugal, G., Viveiros Monteiro, A., Timoteo, A.T., Ribeiro, M., Espregueira Mendes, D., Rodrigues, R., Lopes, R., Ribeiro, V., Melao, F., Magalhaes, D., Silva, J., Ribeiro, V. Gama, Braga, P., Goncalves, M., de Morais, G. Pires, Melica, B., Rodrigues, A., Santos, L., Cojocaru, L., Mazilu, L., Suceveanu, A.I., Rusali, A., Parepa, I.R., Maxim, R., Matei, L., Ioanovici, S., Buzas, R., Mihaela Iuliana, M., Susan, M., Ionita, M., Coceala, L., Suceava, I., Ciobotaru, G., Lighezan, D., Nicolescu, C., Mukhametgatova, D., Baleeva, L., Galyavich, A., Gratsiansky, N.A., Erlikh, A., Kondratenko, V., Libis, R., Vezikova, N., Skopets, I., Marusenko, I., Lapshin, K., Yakovlev, A., Lokhovinina, N., Alugishvili, M., Panov, A., Abesadze, I., Salakhova, J., Kondrateva, I., and Duplyakov, D.
- Published
- 2016
- Full Text
- View/download PDF
5. Effects of cadmium on mitochondrial structure and function in different organs: studies on the soil centipedeLithobius forficatus(Myriapoda, Chilopoda)
- Author
-
Rost-Roszkowska, M., primary, Poprawa, I., additional, Chajec, Ł., additional, Chachulska-Żymełka, A., additional, Wilczek, G., additional, Wilczek, P., additional, Tarnawska, M., additional, Student, S., additional, and Leśniewska, M., additional
- Published
- 2021
- Full Text
- View/download PDF
6. Nickel toxicity in the hindgut of an isopod Porcellio scaber (Oniscidea)
- Author
-
Tarnawska, M., Migula, P., Przybyłowicz, W., Mesjasz-Przybyłowicz, J., and Augustyniak, M.
- Published
- 2007
- Full Text
- View/download PDF
7. Effects of cadmium on mitochondrial structure and function in different organs: studies on the soil centipede Lithobius forficatus (Myriapoda, Chilopoda).
- Author
-
Rost-Roszkowska, M., Poprawa, I., Chajec, Ł., Chachulska-Żymełka, A., Wilczek, G., Wilczek, P., Tarnawska, M., Student, S., and Leśniewska, M.
- Subjects
CADMIUM ,GERM cells ,CELL physiology ,CENTIPEDES ,MYRIAPODA - Abstract
Mitochondria are organelles that play a crucial role in cell physiology, cell death, and aging. They are among the first responders to different stressors that originate from the environment. Cadmium as a heavy metal affects different levels of body organization: from organs through tissues and cells to organelles. Based on our previous research results, we decided to check how the exposure to cadmium affects the functioning of mitochondria in different organs of soil living centipede Lithobius forficatus. The activity of mitochondria in somatic and germ cells has been analyzed using transmission electron microscopy (TEM), confocal microscopy, and flow cytometry. Changes in the mitochondrial membrane potential and mitochondrial dismutase (MnSOD) activity in relation to the accumulation of reactive oxygen species (ROS) caused by cadmium exposure have been studied. Individuals were divided into 3 experimental groups depending on cadmium concentration in soil. Changes in mitochondrial ultrastructure caused by cadmium are tissue-dependent and associated with an increase of ROS levels. The system of ROS and MnSOD activation works more efficiently in the case of gonads than in the digestive system. While the short-term cadmium exposure alters the fine structure of both the somatic and germ-line cells in gonads, the long-term cadmium exposure causes mitochondrial ultrastructure regeneration. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
8. Immune response of juvenile common carp (Cyprinus carpio L.) exposed to a mixture of sewage chemicals
- Author
-
Tarnawska, M., primary, Augustyniak, M., additional, Łaszczyca, P., additional, Migula, P, additional, Irnazarow, I., additional, Krzyżowski, M., additional, and Babczyńska, A., additional
- Published
- 2019
- Full Text
- View/download PDF
9. Cardiopoietic cell therapy for advanced ischemic heart failure : results at 39 weeks of the prospective, randomized, double blind, sham-controlled CHART-1 clinical trial
- Author
-
Bartunek, Jozef, Terzic, Andre, Davison, Beth A, Filippatos, Gerasimos S, Radovanovic, Slavica, Beleslin, Branko, Merkely, Bela, Musialek, Piotr, Wojakowski, Wojciech, Andreka, Peter, Horvath, Ivan G, Katz, Amos, Dolatabadi, Dariouch, El Nakadi, Badih, Arandjelovic, Aleksandra, Edes, Istvan, Seferovic, Petar M, Obradovic, Slobodan, Vanderheyden, Marc, Jagic, Nikola, Petrov, Ivo, Atar, Shaul, Halabi, Majdi, Gelev, Valeri L, Shochat, Michael K, Kasprzak, Jaroslaw D, Sanz Ruiz, Ricardo, Heyndrickx, Guy R, Nyolczas, Noémi, Legrand, Victor, Guédès, Antoine, Heyse, Alex, Moccetti, Tiziano, Fernandez Aviles, Francisco, Jimenez Quevedo, Pilar, Bayes Genis, Antoni, Hernandez Garcia, Jose Maria, Ribichini, Flavio, Gruchala, Marcin, Waldman, Scott A, Teerlink, John R, Gersh, Bernard J, Povsic, Thomas J, Henry, Timothy D, Metra, Marco, Hajjar, Roger J, Tendera, Michal, Behfar, Atta, Alexandre, Bertrand, Seron, Aymeric, Stough, Wendy Gattis, Sherman, Warren, Cotter, Gad, Wijns, W. i. l. l. i. a. m. Collaborators Clinical investigators, Dens, sites Belgium: Ziekenhuis Oost Limburg: J., Dupont, M., Mullens, W., Janssens, M., Dolatabadi, Hoˆpital Civil de Charleroi: D., De Bruyne, Y., Lalmand, J., Dubois, P., El Nakadi, B., Aminian, A., De Vuyst, E., Gurnet, P., Gujic, M., Blankoff, I., Guedes, CHU Mont Godinne UCL: A., Gabriel, L., Seldrum, S., Doyen, C., Andre´, M., Heyse, AZ Glorieux: A., Van Durme, F., Verschuere, J., Legrand, Domaine Universitaire du Sart Tilman: V., Gach, O., D’Orio, V., Davin, L., Lancellotti, P., Baudoux, E., Ancion, A., Dulgheru, R., Vanderheyden, OLV Ziekenhuis Aalst – Cardiologie: M., Bartunek, J., Wijns, W., Verstreken, S., Penicka, . M., Gelev, P. Meeus Bulgaria: Tokuda Hospital Sofia: V., Zheleva Kichukova, I., Parapunova, R., Melamed, R., Sardovski, S., Radev, O., Yordanov, A., Radinov, A., Nenov, D., Amine, I., Petrov, City Hospital Clinic Cardiology Center: I., Kichukov, K., Nikitasov, L., Stankov, Z., Stoyanov, H., Tasheva Dimitrova, I., Angelova, M., Dimitrov, E., Minchev, M., Garvanski, I., Botev, C., Polomski, P., Alexandrovska University Hospital, Vassilev, Sofia: D., Karamfiloff, K., Tarnovska Kadreva, R., Vladimirova, L., Dimitrov, G., Hadzhiev, E., Tzvetkova, G., Andreka, . M. Atanasova Hungary: Gottsegen Gyo¨ rgy Orszagos Kardiologiai Inte´zet: P., Fontos, G., Fabian, J., Csepregi, A., Uzonyi, G., Gelei, A., Edes, Debreceni Egyetem Orvos e´s Ege´szse´gtudomanyi Centrum Altalanos Orvostudomanyi Kar Kardiologia Inte´zet: I., Balogh, L., Vajda, G., Darago, A., Gergely, S., Fulop, T., Jenei, C., Horvath, Pe´csi Tudomanyegyetem Klinikai Ko¨zpont Szıvgyogyaszati Klinika: I., Magyari, B., Nagy, A., Cziraki, A., Faludi, R., Kittka, B., Alizadeh, H., Merkely, Semmelweis Egyetem Varosmajori Szıv e´s Ergyogyaszati Klinika: B., Geller, L., Farkas, P., Szombath, G., Foldes, G., Skopal, J., Kovacs, A., Kosztin, A., Gara, E., Sydo, N., Nyolczas, MH Ege´szse´gu¨gyi Ko¨zpont Kardiologiai Osztaly: N., Kerecsen, G., Korda, A., Kiss, . M., Borsanyi, T., Polgar, B., Muk, B., Sharif, Z. Bari Ireland: HRB Clinical Research Facility: F., Atar, Y. M. Smyth Israel:Western Galilee Hospital: S., Shturman, A., Akria, L., Kilimnik, M., Brezins, M., Halabi, Ziv Medical Center: M., Dally, N., Goldberg, A., Aehab, K., Rosenfeld, I., Levinas, T., Saleem, D., Katz, Barzilai Medical Center: A., Plaev, T., Drogenikov, T., Nemetz, A., Barshay, Y., Jafari, J., Orlov, I., Nazareth Hospital EMMS: M. Omory, N. Kogan Nielsen, Shochat, Hillel Yaffe Medical Center: M., Shotan, A., Frimerman, A., Meisel, S., Asif, A., Sofer, O., Blondheim, D. S., Vazan, A., Metra, L. Arobov Italy: A. O. Spedali Civili di Brescia: M., Bonadei, I., Inama, L., Chiari, E., Lombardi, C., Magatelli, M., Russo, D., Lazzarini, V., Carubelli, V., Vassanelli, AOUI Verona – Borgo Trento Hospital: C., Ribichini, Flavio Luciano, Bergamini, C., Krampera, Mauro, Cicoria, M. A., Zanolla, L., Dalla Mura, D., Gambaro, A., Rossi, A., Pesarini Poland: Jagiellonian University Department of Cardiac, G., Musialek, Vascular Diseases at John Paul II Hospital in Krakow: P., Mazurek, A., Drabik, L., Ka˛dzielski, A., Walter, Z., Dzieciuch Rojek, M., Rubis, P., Plazak, . W., Tekieli, L., Podolec, J., Orczyk, W., Sutor, U., Zmudka, K., Olszowska, M., Podolec, P., Gruchala, Uniwersyteckie Centrum Kliniczne: M., Ciecwierz, D., Mielczarek, M., Burakowski, S., Chmielecki, M., Zielinska, M., Frankiewicz, A., Wdowczyk, J., Stopczynska, I., Bellwon, J., Mosakowska, K., Nadolna, R., Wroblewska, J., Rozmyslowska, M., Rynkiewicz, M., Marciniak, I., Raczak, G., Tarnawska, M., Taszner, M., Kasprzak, Bieganski Hospital: J., Plewka, M., Fiutowska, D., Rechcinski, T., Lipiec, P., Sobczak, M., Weijner Mik, P., Wraga, M., Krecki, R., Markiewicz, M., Haval Qawoq, D., Wojakowski, Gornosla˛skie Centrum Medyczne Sla˛skie j. Akademii Medycznej: W., Ciosek, J., Dworowy, S., Gaszewska Zurek, E., Ochala, A., Cybulski, W., Jadczyk, T., Wanha, W., Parma, Z., Kozlowski, M., Dzierzak, M., Markiewicz Serbia: Clinical Hospital Center Zvezdara, M., Arandjelovic, Cardiology Clinic: A., Sekularac, N., Boljevic, D., Bogdanovic, A., Zivkovic, S., Cvetinovic, N., Loncar, G., Clinical Centre of Serbia, Beleslin, Cardiology Clinic: B., Nedeljkovic, M., Trifunovic, D., Giga, V., Banovic, M., Nedeljkovic, I., Stepanovic, J., Vukcevic, V., Djordjevic Dikic, A., Dobric, M., Obrenovic Kircanski, B., Seferovic, Cardiology Clinic: P., Orlic, D., Tesic, M., Petrovic, O., Milinkovic, I., Simeunovic, D., Jagic, Clinical Center of Kragujevac: N., Tasic, M., Nikolic, D., Miloradovic, V., Djurdjevic, P., Sreckovic, M., Zornic, N., Clinical Hospital Center Bezanijska Kosa, Radovanovic, Cardiology Department: S., Saric, J., Hinic, S., Djokovic, A., Ðordevic, S., Bisenic, V., Markovic, O., Stamenkovic, S., Malenkovic, V., Tresnjak, J., Misic, G., Cotra, D., Tomovic, L., Vuckovic, V., Clinic of Emergency Internal Medicine, Obradovic, Military Medical Academy: S., Jovic, Z., Vukotic, S., Markovic, D., Djenic, N., Ristic Andjelkov, A., Bayes Genis, D. Ljubinka Spain: Hospital Universitario Germans Trias I. Pujol: A., Rodriguez Leor, O., Labata, C., Vallejo, N., Ferrer, E., Batlle, M., Fernandez Aviles, Hospital General Universitario Gregorio Mara~non: F., Sanz Ruiz, R., Casado, A., Loughlin, G., Zatarain, E., Anguita, J., Ferna ndez Santos, M. E., Pascual, C., Bermejo, J., Hernandez Garcia, Hospital Clinico Universitario Virgen de la Victoria: J. M., Jimenez Navarro, M., Dominguez, A., Carrasco, F., Mu~noz, A., Garcia Pinilla, J. M., Ruiz, J., Queipo de Llano, M. P., Hernandez, A., Fernandez, A., Jimenez Quevedo, Hospital Clinico San Carlos: P., Guerra, R., Biagioni, C., Gonzalez, R. A., Gomez deDiego, J. J., Mansson Broberg, L. Perez de Isla Sweden: Karolinska University Hospital: A., Sylve´n, C., Leblanc, K., Winter, R., Blomberg, P., Gunyeli, E., Ruck, A., Silva, C., Fo¨rstedt Switzerland: CardioCentro Ticino, J., Moccetti, Switzerland: T., Rossi, M., Pasotti, E., Petrova, I., Crljenica, C., Monti, C., Murzilli, R., Su¨rder, D., Moccetti, M., Turchetto, L., Locicero, V., Chiumiento, L., Maspoli, S., Mombelli, M., Anesini, A., Biggiogero, M., Ponti, G., Camporini, C., Polledri, S., Hill, G. Dolci United Kingdom: Kings College Hospital: J., Plymen, C., Amin Youssef, G., Mcdonagh, T., Drasar, E., Mijovic, A., Jouhra, F., Mcloman, D., Dworakowski, R., Webb, I., Byrne, J., and Potter, V.
- Subjects
0301 basic medicine ,Male ,Cardiopoiesis ,Cardiovascular disease ,Disease severity ,Marker ,Precision medicine ,Regenerative medicine ,Stem cell ,Target population ,Adult ,Aged ,Double-Blind Method ,Female ,Heart Failure ,Humans ,Mesenchymal Stem Cell Transplantation ,Middle Aged ,Myocardial Ischemia ,Prospective Studies ,Treatment Outcome ,Young Adult ,Cardiology and Cardiovascular Medicine ,Cell- and Tissue-Based Therapy ,mesenchymal stem-cells ,030204 cardiovascular system & hematology ,Cardiorespiratory Medicine and Haematology ,outcomes ,Fast-Track Clinical Research ,Sudden cardiac death ,0302 clinical medicine ,Ischemia ,cardiovascular disease ,Clinical endpoint ,target population ,CHART Program ,Ejection fraction ,bone-marrow ,Heart Failure/Cardiomyopathy ,3. Good health ,Cohort ,Cardiology ,Fast Track ,disease severity ,delivery ,medicine.medical_specialty ,precision medicine ,Clinical Sciences ,regenerative medicine ,03 medical and health sciences ,cardiopoiesis ,Internal medicine ,medicine ,Adverse effect ,marker ,disease ,business.industry ,medicine.disease ,mortality ,Confidence interval ,Clinical trial ,stem cell ,Editor's Choice ,030104 developmental biology ,predictors ,Cardiovascular System & Hematology ,Heart failure ,business - Abstract
Altres ajuts: This work was supported by Celyad, SA (Mont-Saint-Guibert, Belgium). Celyad has received research grants from the Walloon Region (Belgium, DG06 funding). Cardiopoietic cells, produced through cardiogenic conditioning of patients' mesenchymal stem cells, have shown preliminary efficacy. The Congestive Heart Failure Cardiopoietic Regenerative Therapy (CHART-1) trial aimed to validate cardiopoiesis-based biotherapy in a larger heart failure cohort. This multinational, randomized, double-blind, sham-controlled study was conducted in 39 hospitals. Patients with symptomatic ischaemic heart failure on guideline-directed therapy (n = 484) were screened; n = 348 underwent bone marrow harvest and mesenchymal stem cell expansion. Those achieving > 24 million mesenchymal stem cells (n = 315) were randomized to cardiopoietic cells delivered endomyocardially with a retention-enhanced catheter (n = 157) or sham procedure (n = 158). Procedures were performed as randomized in 271 patients (n = 120 cardiopoietic cells, n = 151 sham). The primary efficacy endpoint was a Finkelstein–Schoenfeld hierarchical composite (all-cause mortality, worsening heart failure, Minnesota Living with Heart Failure Questionnaire score, 6-min walk distance, left ventricular end-systolic volume, and ejection fraction) at 39 weeks. The primary outcome was neutral (Mann–Whitney estimator 0.54, 95% confidence interval [CI] 0.47–0.61 [value > 0.5 favours cell treatment], P = 0.27). Exploratory analyses suggested a benefit of cell treatment on the primary composite in patients with baseline left ventricular end-diastolic volume 200–370 mL (60% of patients) (Mann–Whitney estimator 0.61, 95% CI 0.52–0.70, P = 0.015). No difference was observed in serious adverse events. One (0.9%) cardiopoietic cell patient and 9 (5.4%) sham patients experienced aborted or sudden cardiac death. The primary endpoint was neutral, with safety demonstrated across the cohort. Further evaluation of cardiopoietic cell therapy in patients with elevated end-diastolic volume is warranted.
- Published
- 2017
10. Effects of cadmium on mitochondrial structure and function in different organs: studies on the soil centipede Lithobius forficatus(Myriapoda, Chilopoda)
- Author
-
Rost-Roszkowska, M., Poprawa, I., Chajec, Ł., Chachulska-Żymełka, A., Wilczek, G., Wilczek, P., Tarnawska, M., Student, S., and Leśniewska, M.
- Abstract
AbstractMitochondria are organelles that play a crucial role in cell physiology, cell death, and aging. They are among the first responders to different stressors that originate from the environment. Cadmium as a heavy metal affects different levels of body organization: from organs through tissues and cells to organelles. Based on our previous research results, we decided to check how the exposure to cadmium affects the functioning of mitochondria in different organs of soil living centipede Lithobius forficatus. The activity of mitochondria in somatic and germ cells has been analyzed using transmission electron microscopy (TEM), confocal microscopy, and flow cytometry. Changes in the mitochondrial membrane potential and mitochondrial dismutase (MnSOD) activity in relation to the accumulation of reactive oxygen species (ROS) caused by cadmium exposure have been studied. Individuals were divided into 3 experimental groups depending on cadmium concentration in soil. Changes in mitochondrial ultrastructure caused by cadmium are tissue-dependent and associated with an increase of ROS levels. The system of ROS and MnSOD activation works more efficiently in the case of gonads than in the digestive system. While the short-term cadmium exposure alters the fine structure of both the somatic and germ-line cells in gonads, the long-term cadmium exposure causes mitochondrial ultrastructure regeneration.
- Published
- 2021
- Full Text
- View/download PDF
11. Epilachna nylanderi, a phytophagus beetle of Ni-hyperaccumulator Berkheya coddii
- Author
-
Mesjasz-Przybylowicz, Jolanta, Orlowska, Elzbieta Zofia, Augustyniak, M., Nakonieczny, M., Tarnawska, M., Przybylowicz, W., Makagele, M., and Migula, P.
- Published
- 2011
12. Population dynamics of Western corn rootworm beetles on different varieties of maize identified using pheromone and floral baited traps
- Author
-
BEREŚ, P. K., primary, DRZEWIECKI, S., additional, NAKONIECZNY, M., additional, TARNAWSKA, M., additional, GUZIK, J., additional, and MIGULA, P., additional
- Published
- 2015
- Full Text
- View/download PDF
13. DNA damage in grasshopper Chorthippus brunneus (Orthoptera) hatchlings following paraquat exposure
- Author
-
Augustyniak, M., primary, Nocoń, Ł., additional, Kędziorski, A., additional, Łaszczyca, P., additional, Sawczyn, T., additional, Tarnawska, M., additional, and Zawisza-Raszka, A., additional
- Published
- 2015
- Full Text
- View/download PDF
14. Micro-PIXE studies of Ni-elimination strategies in representatives of two families of beetles feeding on Ni-hyperaccumulating plant Berkheya coddii
- Author
-
Migula, P., primary, Przybyłowicz, W. J., additional, Nakonieczny, M., additional, Augustyniak, M., additional, Tarnawska, M., additional, and Mesjasz-Przybyłowicz, J., additional
- Published
- 2011
- Full Text
- View/download PDF
15. Nuclear microprobe studies of grasshopper feeding on nickel hyperaccumulating plants
- Author
-
Augustyniak, M., primary, Przybyłowicz, W., additional, Mesjasz‐Przybyłowicz, J., additional, Tarnawska, M., additional, Migula, P., additional, Głowacka, E., additional, and Babczyńska, A., additional
- Published
- 2008
- Full Text
- View/download PDF
16. Short-term effects of dimethoate on metabolic responses in Chrysolina pardalina (Chrysomelidae) feeding on Berkheya coddii (Asteraceae), a hyper-accumulator of nickel
- Author
-
Augustyniak, M., primary, Migula, P., additional, Mesjasz-Przybyłowicz, J., additional, Tarnawska, M., additional, Nakonieczny, M., additional, Babczyńska, A., additional, Przybyłowicz, W., additional, and Augustyniak, M.G., additional
- Published
- 2007
- Full Text
- View/download PDF
17. Micro-PIXE in ecophysiology
- Author
-
Przybyłowicz, W. J., primary, Mesjasz-Przybyłowicz, J., additional, Migula, P., additional, Nakonieczny, M., additional, Augustyniak, M., additional, Tarnawska, M., additional, Turnau, K., additional, Ryszka, P., additional, Orłowska, E., additional, Zubek, Sz., additional, and Głowacka, E., additional
- Published
- 2005
- Full Text
- View/download PDF
18. Uptake of cadmium, lead, nickel and zinc from soil and water solutions by the nickel hyperaccumulator berkheya coddii
- Author
-
Mesjasz-Przybyłowics, J., Nakonieczny, M., Migula, P., Maria Augustyniak, Tarnawska, M., Reimold, W. U., Koeberl, C., Przybyłowicz, W., and Głowacka, E.
19. Survival, growth and digestive functions after exposure to nanodiamonds - Transgenerational effects beyond contact time in house cricket strains.
- Author
-
Augustyniak M, Ajay AK, Kędziorski A, Tarnawska M, Rost-Roszkowska M, Flasz B, Babczyńska A, Mazur B, Rozpędek K, Alian RS, Skowronek M, Świerczek E, Wiśniewska K, and Ziętara P
- Subjects
- Animals, Cell Line, Time Factors, Nanodiamonds toxicity, Gryllidae physiology
- Abstract
The long-term exposure effects of nanodiamonds (NDs), spanning an organism's entire lifespan and continuing for subsequent generation, remain understudied. Most research has focused on evaluating their biological impacts on cell lines and selected organisms, typically over short exposure durations lasting hours or days. The study aimed to assess growth, mortality, and digestive functions in wild (H) and long-lived (D) strains of Acheta domesticus (Insecta: Orthoptera) after two-generational exposure to NDs in concentrations of 0.2 or 2 mg kg
-1 of food, followed by their elimination in the third generation. NDs induced subtle stimulating effect that depended on the strain and generation. In the first generation, more such responses occurred in the H than in the D strain. In the first generation of H strain insects, contact with NDs increased survival, stimulated the growth of young larvae, and the activity of most digestive enzymes in mature adults. The same doses and exposure time did not cause similar effects in the D strain. In the first generation of D strain insects, survival and growth were unaffected by NDs, whereas, in the second generation, significant stimulation of those parameters was visible. Selection towards longevity appears to support higher resistance of the insects to exposure to additional stressor, at least in the first generation. The cessation of ND exposure in the third generation caused potentially harmful changes, which included, e.g., decreased survival probability in H strain insects, slowed growth of both strains, as well as changes in heterochromatin density and distribution in nuclei of the gut cells in both strains. Such a reaction may suggest the involvement of epigenetic inheritance mechanisms, which may become inadequate after the stress factor is removed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
20. Adaptation by death? Cell death-based tolerance to cadmium in 150-generation exposure of Spodoptera exiqua Hübner (Lepidoptera: Noctuidae).
- Author
-
Babczyńska A, Rost-Roszkowska M, Kafel A, Łozowski B, Augustyniak M, and Tarnawska M
- Subjects
- Animals, Spodoptera physiology, Larva metabolism, Cell Death, Cadmium toxicity, Cadmium metabolism, Moths metabolism
- Abstract
Mechanisms, including autophagy and apoptosis, which serve to regulate and ensure proper organism functions under optimal conditions, play additional defensive roles under environmental pressure. The aim of this study was to test the following hypotheses: (i) elevated autophagy and apoptosis intensity levels, as defensive processes in response to contact with cadmium, are maintained for a limited number of generations and (ii) the number of generations after which levels of cell death processes reach the reference level depends on selective pressure. Cell death processes were assessed by light and transmission electron microscopy, terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL), and cytometric analyses. Model insects (Spodoptera exiqua, Hübner, 1808) were orally exposed to various concentrations of cadmium for 18 generations and compared with reference strains exposed to cadmium or not (control) for over 150 generations. Elevated programmed cell death intensity levels decreased after several generations, indicating tolerance of individuals to cadmium in the diet and verifying the first hypothesis; however, testing the second hypothesis indicated that the number of generations depended not only on pressure intensity, but also on cell death type, since levels of autophagy remained increased for a minimum of 12 generations., (© The Author(s) 2023. Published by Oxford University Press on behalf of Entomological Society of America.)
- Published
- 2023
- Full Text
- View/download PDF
21. Multigenerational Effects of Graphene Oxide Nanoparticles on Acheta domesticus DNA Stability.
- Author
-
Flasz B, Ajay AK, Tarnawska M, Babczyńska A, Majchrzycki Ł, Kędziorski A, Napora-Rutkowski Ł, Świerczek E, and Augustyniak M
- Subjects
- Animals, 8-Hydroxy-2'-Deoxyguanosine, DNA, Gryllidae genetics, Environmental Pollutants, Nanoparticles
- Abstract
The use of nanoparticles like graphene oxide (GO) in nanocomposite industries is growing very fast. There is a strong concern that GO can enter the environment and become nanopollutatnt. Environmental pollutants' exposure usually relates to low concentrations but may last for a long time and impact following generations. Attention should be paid to the effects of nanoparticles, especially on the DNA stability passed on to the offspring. We investigated the multigenerational effects on two strains (wild and long-lived) of house cricket intoxicated with low GO concentrations over five generations, followed by one recovery generation. Our investigation focused on oxidative stress parameters, specifically AP sites (apurinic/apyrimidinic sites) and 8-OHdG (8-hydroxy-2'-deoxyguanosine), and examined the global DNA methylation pattern. Five intoxicated generations were able to overcome the oxidative stress, showing that relatively low doses of GO have a moderate effect on the house cricket (8-OHdG and AP sites). The last recovery generation that experienced a transition from contaminated to uncontaminated food presented greater DNA damage. The pattern of DNA methylation was comparable in every generation, suggesting that other epigenetic mechanisms might be involved.
- Published
- 2023
- Full Text
- View/download PDF
22. Joint cadmium and polypropylene microparticle action in cadmium tolerant model insect.
- Author
-
Babczyńska A, Górka M, Lis A, Tarnawska M, Łozowski B, Brożek J, Rozpędek K, Augustyniak M, Skowronek M, and Kafel A
- Subjects
- Animals, Plastics, Heat-Shock Proteins, Spodoptera, Biomarkers metabolism, Cadmium toxicity, Cadmium metabolism, Polypropylenes metabolism
- Abstract
Microplastic enlisted as a contaminant of emerging concerns in polluted environments interact with "traditional" contaminants such as metals, causing, among others, their increased accumulation in the body. Harmful effects depend on the exposed animals' possible preadaptation and/or cross-tolerance. The project aimed to assess the role of this phenomenon in the limited toxicity of polypropylene fibers (PPf) in 0%, 0.02%, 0.06, 0.18%, 0.54%, and 1.6% of Cd-supplemented food of larvae of Spodoptera exigua multigenerationally selected to cadmium tolerance. The activity of 20 digestive enzymes (API-ZYM test), defensins, and heat shock proteins, HSP70 levels in the exposed groups were used as biomarkers. PPfs caused the increase of Cd accumulation in the body, while intake of polypropylene microfibers did not change the biomarker levels. Moreover, multigenerational Cd pre-exposure, due to increased tolerance of Cd and, possibly, cross-tolerance, prepares the insects for an additional stressor (PPf) alone and in interaction with cadmium., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
23. Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus .
- Author
-
Flasz B, Dziewięcka M, Ajay AK, Tarnawska M, Babczyńska A, Kędziorski A, Napora-Rutkowski Ł, Ziętara P, Świerczek E, and Augustyniak M
- Subjects
- Humans, DNA Damage, 8-Hydroxy-2'-Deoxyguanosine, DNA Repair, Longevity, Graphite toxicity
- Abstract
The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage, double-strand breaks-DSB, 8-hydroxy-2'-deoxyguanosine-8-OHdG, abasic site-AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus . Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms.
- Published
- 2022
- Full Text
- View/download PDF
24. Does age pay off? Effects of three-generational experiments of nanodiamond exposure and withdrawal in wild and longevity-selected model animals.
- Author
-
Augustyniak M, Babczyńska A, Dziewięcka M, Flasz B, Karpeta-Kaczmarek J, Kędziorski A, Mazur B, Rozpędek K, Seyed Alian R, Skowronek M, Świerczek E, Świętek A, Tarnawska M, Wiśniewska K, and Ziętara P
- Subjects
- Animals, Antioxidants metabolism, Defensins metabolism, Defensins pharmacology, HSP70 Heat-Shock Proteins genetics, HSP70 Heat-Shock Proteins metabolism, Longevity, Oxidative Stress, Reactive Oxygen Species metabolism, Nanodiamonds
- Abstract
Nanodiamonds (NDs) are considered a material with low toxicity. However, no studies describe the effects of ND withdrawal after multigenerational exposure. The aim was to evaluate ND exposure (in the 1st and 2nd generations) effects at low concentrations (0.2 or 2 mg kg
-1 ) and withdrawal (in the 3rd generation) in the wild (H) and longevity-selected (D) model insect Acheta domesticus. We measured selected oxidative stress parameters, immunity, types of cell death, and DNA damage. Most of the results obtained in the 1st generation, e.g., catalase (CAT), total antioxidant capacity (TAC), heat shock proteins (HSP70), defensins, or apoptosis level, confirmed no significant toxicity of low doses of NDs. Interestingly, strain-specific differences were observed. D-strain crickets reduced autophagy, the number of ROS+ cells, and DNA damage. The effect can be a symptom of mobilization of the organism and stimulation of physiological defense mechanisms in long-living organisms. The 2nd-generation D-strain insects fed ND-spiked food at higher concentrations manifested a reduction in CAT, TAC, early apoptosis, and DNA damage, together with an increase in HSP70 and defensins. ROS+ cells and cells with reduced membrane potential and autophagy did not differ significantly from the control. H-strain insects revealed a higher number of ROS+ cells and cells with reduced membrane potential, decreased CAT activity, and early apoptosis. Elimination of NDs from the diet in the 3rd generation did not cause full recovery of the measured parameters. We noticed an increase in the concentration of HSP70 and defensins (H-strain) and a decrease in apoptosis (D-strain). However, the most visible increase was a significant increase in DNA damage, especially in H-strain individuals. The results suggest prolonged adverse effects of NDs on cellular functions, reaching beyond "contact time" with these particles. Unintentional and/or uncontrolled ND pollution of the environment poses a new challenge for all organisms inhabiting it, particularly during multigenerational exposure., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)- Published
- 2022
- Full Text
- View/download PDF
25. Multigenerational selection towards longevity changes the protective role of vitamin C against graphene oxide-induced oxidative stress in house crickets.
- Author
-
Flasz B, Dziewięcka M, Kędziorski A, Tarnawska M, Augustyniak J, and Augustyniak M
- Subjects
- Animals, Ascorbic Acid, Longevity, Oxidative Stress, Graphite toxicity, Gryllidae
- Abstract
This research was designed to investigate changes that can arise in an invertebrate organism due to stress caused by a strong prooxidant, graphene oxide (GO), and a potent antioxidant, vitamin C. The study aimed to investigate if vitamin C may support convalescence after chronic GO intoxication. We investigated the toxicity of chronic dietary graphene oxide administration in house cricket (Acheta domesticus) types: wild and selected for longevity (with a better developed antioxidant system, conducive to long life). Vitamin C was applied immediately after cessation of graphene oxide intoxication to check if it can support the remedial effect. The condition of cells, DNA stability, catalase activity, and the reproduction potential, measured as the Vitellogenin (Vg) protein expression level, were investigated in control and GO treated groups, recovery groups (-GO), and recovery groups with Vit. C (-GO + Vit.C). In this study vitamin C had no evident remedial effect on the house crickets exposed to graphene oxide. Most probably, the mechanism of vitamin C action, in case of intoxication with nanoparticles, is much more complicated. In the context of the results obtained, it is worth considering whether Vit. C, applied after GO intoxication, causes further disturbance of homeostasis in terms of the cells' redox potential., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
26. The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
- Author
-
Francikowski J, Potrzebska M, Szulińska E, Tarnawska M, Radai Z, Łozowski B, Baran B, and Krzyżowski M
- Subjects
- Animals, Female, Glycogen metabolism, Gryllidae genetics, Gryllidae growth & development, Male, Mutation, Pigmentation genetics, Diet, Gryllidae metabolism, Kynurenine metabolism, Monosaccharides metabolism, Tryptophan metabolism
- Abstract
Tryptophan (TRP) is one of the essential amino acids in the animal body. Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level. It has been shown to have a number of essential functions, such as in the production of other biologically active molecules. The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye. This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition. A parallel goal was to compare the response of both cricket strains to HMD. ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio. Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content. A decrease in food intake was also observed in the groups on HMD. However, no changes in imago body weight and water content were observed. The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain. At the same time, a stronger detrimental effect of fructose than of glucose was apparent. Sex was found to be a modulating factor in the response to HMD., (© 2021. The Author(s).)
- Published
- 2021
- Full Text
- View/download PDF
27. High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese Sprague-Dawley Rats.
- Author
-
Poloczek J, Tarnawska M, Chełmecka E, Łaszczyca P, Gumprecht J, and Stygar D
- Subjects
- Animals, Blood Glucose metabolism, Diet, Carbohydrate Loading adverse effects, Diet, High-Fat adverse effects, Dietary Sugars adverse effects, Disease Models, Animal, Duodenum surgery, Glucose Tolerance Test, Jejunum surgery, Liver metabolism, Obesity etiology, Obesity surgery, Rats, Rats, Sprague-Dawley, Bariatric Surgery methods, C-Reactive Protein metabolism, Fetuin-B metabolism, Growth Differentiation Factor 15 blood, Obesity blood, Serum Amyloid P-Component metabolism
- Abstract
The liver plays a central role in glucose and fatty acid metabolism and acts as an endocrine organ that secretes hepatokines with diverse systemic effects. The study aimed to examine the influence of duodenojejunal omega switch (DJOS) bariatric surgery in combination with different diets on glucose administration parameters and hepatokines levels. After 8 weeks on high fat, high sugar diet (HFS) or control diets (CD), Sprague-Dawley rats underwent DJOS or SHAM (control) surgery. For the next 8 weeks after the surgery, half of DJOS and SHAM-operated animals were kept on the same diet as before, and half had a diet change. The oral glucose tolerance test (OGTT) was performed three times: 8 weeks before and 4 and 8 weeks after surgery. Fetuin-B, growth differentiation factor-15 (GDF-15), pentraxin 3 (PTX3) plasma levels were analyzed. DJOS surgery had a beneficial effect on oral glucose tolerance test (OGTT) results and the area under the curve (AUC
OGTT ). The OGTT results depended on the time elapsed after the surgery, the type of diet used, the surgery performed, and the interaction between these factors. DJOS bariatric surgery reduced fetuin-B and GDF15 plasma levels. Interaction between the type of surgery performed and diet used influenced the fetuin-B and PTX-3 plasma levels. A dietary regime is essential to achieve therapeutic and clinical goals after bariatric surgery.- Published
- 2021
- Full Text
- View/download PDF
28. Multigenerational graphene oxide intoxication results in reproduction disorders at the molecular level of vitellogenin protein expression in Acheta domesticus.
- Author
-
Flasz B, Dziewięcka M, Kędziorski A, Tarnawska M, and Augustyniak M
- Subjects
- Female, Humans, Proteomics, Reproduction, Graphite toxicity, Vitellogenins genetics
- Abstract
The anthropogenic activities may lead to accumulation of graphene oxide (GO) pollution in the environment. Organisms exposed to chronic or multigenerational GO intoxication can present reproduction depletion. Vitellogenin (Vg) has been used as a parameter for evaluating female fertility due to its importance in embryo nutrition. In this study, we used a promising model organism, Acheta domesticus, which was intoxicated with GO in food for three generations. The aim of the study was to investigate the process of Vg synthesis in crickets depending on the exposure time, GO concentration, and age of the females. The results revealed that chronic GO intoxication had adverse effects on the Vg expression pattern. The 1st generation of insects showing low Vg expression was most affected. The 2nd generation of A. domesticus presented a high Vg expression. The last investigated generation seemed to cope with stress caused by GO, and the Vg expression was balanced. We suggest that the epigenetic mechanisms may play a role in the information transfer to the next generations on how to react to the risk factor and keep reproduction at a high rate. We suspect that chronic GO intoxication can disturb the regular formation of the Vg quaternary structure, resulting in consequences for developing an embryo., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
29. Energy reserves, oxidative stress and development traits of Spodoptera exigua Hübner individuals from cadmium strain.
- Author
-
Kafel A, Babczyńska A, Zawisza-Raszka A, Tarnawska M, Płachetka-Bożek A, and Augustyniak M
- Subjects
- Animals, Antioxidants, Humans, Larva, Spodoptera, Cadmium toxicity, Oxidative Stress
- Abstract
Cadmium as a common environmental stressor may exert highly toxic effects on herbivorous insects. The question was whether possible elevation of an oxidative stress and imbalance of energetic reserves in insects may depend on developmental stage, sex and insect population's multigenerational history of exposure to cadmium. So, the aim of this study was to compare of the development traits, total antioxidant capacity, lipid peroxidation, RSSR to RSH ratio and the concentration of carbohydrates, glycogen, lipids and proteins in whole individuals (larvae or pupae) of Spodoptera exigua originating from two strains: control and selected over 120 generations with sublethal metal concentration (44 Cd mg per dry weight of diet). Generally, the increase of the protein, carbohydrates, glycogen concentration and lipid peroxidation decrease with age of the larvae were found. Revealed cases of a higher mobilisation of carbohydrates and proteins, and changes in total antioxidant capacity or lipid peroxidation, in individuals being under metal exposure, occurred in strain-depended mode. Short-term Cd exposure effect was connected with possible higher engagement of proteins and glycogen in detoxification processes, but also higher concentration of lipid peroxidation. In turn, for long-term Cd exposure effect lower lipids concentration and higher thiols usage seemed to be more specific., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020. Published by Elsevier Ltd.)
- Published
- 2021
- Full Text
- View/download PDF
30. Autophagy: a necessary defense against extreme cadmium intoxication in a multigenerational 2D experiment.
- Author
-
Babczyńska A, Nowak A, Kafel A, Łozowski B, Rost-Roszkowska M, Tarnawska M, Augustyniak M, Sawadro M, and Molenda A
- Subjects
- Animals, Environmental Pollutants toxicity, Larva drug effects, Larva growth & development, Spodoptera growth & development, Spodoptera physiology, Autophagy drug effects, Cadmium toxicity, Cadmium Poisoning pathology, Spodoptera drug effects
- Abstract
Autophagy is a natural process that aims to eliminate malfunctioning cell parts, organelles or molecules under physiological conditions. It is also induced in response to infection, starvation or oxidative stress to provide energy in case of an energy deficit. The aim of this 2-dimensional study was to test if, and if so, how, this process depends on the concentration of cadmium in food (with Cd concentrations from 0 to 352 μg of Cd per g of food (dry weight)-D1 dimension) and the history of selection pressure (160 vs 20 generations of exposure to Cd-D2 dimension). For the study, the 5th instar larvae of a unique strain of the moth Spodoptera exigua that was selected for cadmium tolerance for 160 generations (44 μg of Cd per g of food (dry weight)), as well as 20-generation (11, 22 and 44 μg of Cd per g of food (dry weight)) and control strains, were used. Autophagy intensity was measured by means of flow cytometry and compared with life history parameters: survivability and duration of the 3rd larval stage. The highest values of autophagy markers were found in the groups exposed to the highest Cd concentration and corresponded (with a significant correlation coefficient) to an increased development duration or decreased survivorship in the respective groups. In conclusion, autophagy is probably initiated only if any other defense mechanisms, e.g., antioxidative mechanisms, are not efficient. Moreover, in individuals from pre-exposed populations, the intensity of autophagy is lower.
- Published
- 2020
- Full Text
- View/download PDF
31. DNA damage in Spodoptera exigua after multigenerational cadmium exposure - A trade-off between genome stability and adaptation.
- Author
-
Augustyniak M, Tarnawska M, Dziewięcka M, Kafel A, Rost-Roszkowska M, and Babczyńska A
- Subjects
- Animals, Genomic Instability, Humans, Larva, Spodoptera genetics, Cadmium toxicity, DNA Damage
- Abstract
Human activity is a serious cause of extensive changes in the environment and a constant reason for the emergence of new stress factors. Thus, to survive and reproduce, organisms must constantly implement a program of adaptation to continuously changing conditions. The research presented here is focused on tracking slow changes occurring in Spodoptera exigua (Lepidoptera: Noctuidae) caused by multigenerational exposure to sub-lethal cadmium doses. The insects received food containing cadmium at concentrations of 5, 11, 22 and 44 μg per g of dry mass of food. The level of DNA stability was monitored by a comet assay in subsequent generations up to the 36th generation. In the first three generations, the level of DNA damage was high, especially in the groups receiving higher doses of cadmium in the diet. In the fourth generation, a significant reduction in the level of DNA damage was observed, which could indicate that the desired stability of the genome was achieved. Surprisingly, however, in subsequent generations, an alternating increase and decrease was found in DNA stability. The observed cycles of changing DNA stability were longer lasting in insects consuming food with a lower Cd content. Thus, a transient reduction in genome stability can be perceived as an opportunity to increase the number of genotypes that undergo selection. This phenomenon occurs faster if the severity of the stress factor is high but is low enough to allow the population to survive., Competing Interests: Declaration of competing interest The authors declare no competing financial interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
32. Vitellogenin expression, DNA damage, health status of cells and catalase activity in Acheta domesticus selected according to their longevity after graphene oxide treatment.
- Author
-
Flasz B, Dziewięcka M, Kędziorski A, Tarnawska M, and Augustyniak M
- Subjects
- Animals, Catalase, DNA Damage, Ecosystem, Health Status, Longevity, Oxidative Stress, Vitellogenins genetics, Graphite, Gryllidae
- Abstract
The increased use of graphene oxide (GO) raises worrisome questions regarding its possible threat to various ecosystems. Invertebrates represent valuable organisms for environmental studies. The lifespan can influence the ability to cope with toxins, especially those that act via oxidative stress. Two strains of Acheta domesticus, which are selected for longevity, were tested. The main aim was to investigate how GO, when administrated in food, affects: the condition of cells, DNA stability, ROS generation and the reproduction potential (the Vitellogenin (Vg) protein expression). The "recovery effect" - after removing GO from the diet for 15 days - was also measured. The results revealed different responses to GO in the wild (H) and long-living (D) strains. The D strain had a higher catalase activity compared to the H strain on the 25th day of the imago stage. Removing GO from the food resulted in a decrease in the catalase activity to the level of the control. On the 5th day of the imago stage, the H strain had a higher cell mortality than the D strain in the GO-intoxicated groups. There was more DNA damage in the H strain compared to the long-living strain. A remedial effect was seen after the GO was removed from the diet. The total Vg protein expression was higher in the H strain and lower in the D strain. The results indicated a GO concentration-dependent outcome. In both strains, removing the GO from the food led to a high Vg expression. The Vg expression after GO treatment, particularly translation and post-translational processing, should be studied in detail in the future. The D strain of crickets had more specialized mechanisms for maintaining homeostasis than the H strain. Organisms can fight off negative effects of GO, especially when they have systems that are well developed against oxidative stress., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
33. Protective role of zinc in Spodoptera exigua larvae under 135-generational cadmium exposure.
- Author
-
Tarnawska M, Babczyńska A, Hassa K, Kafel A, Płachetka-Bożek A, Augustyniak J, Dziewięcka M, Flasz B, and Augustyniak M
- Subjects
- Animals, Antioxidants metabolism, Beta vulgaris, Drug Tolerance, HSP70 Heat-Shock Proteins, Larva drug effects, Spodoptera drug effects, Adaptation, Physiological, Cadmium toxicity, Soil Pollutants toxicity, Spodoptera physiology, Zinc metabolism
- Abstract
The aim of this study was to investigate whether zinc supplementation modulates cadmium toxicity in the beet armyworm Spodoptera exigua selected for 135 generations towards cadmium tolerance. To achieve this, larvae originating from three laboratory populations of S. exigua (control strain - C; cadmium-intoxicated for 135 generations strain - Cd, and control strain intoxicated with Cd for 1 generation - CCd) were additionally exposed to zinc in three concentrations (Zn1, 400 μg Zn·g
-1 dry mass of food; Zn2; 200 μg Zn·g-1 dry mass of food; Zn3, 100 μg Zn·g-1 dry mass of food). As the markers of toxicity, a life history traits (the duration of L4 and L5 stages), cellular (DNA damage indices) and biochemical parameters (ADP/ATP ratio and ATP and HSP70 concentrations) were chosen. The duration of larval stages of Zn supplemented larvae was prolonged, while cellular and biochemical indicators, in general, appeared to be lower in comparison to the insects from respective reference groups in each laboratory populations. Moreover, the range of the differences depended on zinc concentration in food. We can suspect that zinc supplementation contributed to the protection of S. exigua individuals against negative effects of cadmium intoxication, probably at the cost of growth rate. Significant differences in the response pattern between insects from different laboratory populations indicate that the influence of additional stress factors is dependent on the overall condition of animals and their previous adaptation to other stressors., (Copyright © 2019 Elsevier Ltd. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
34. Relationship between ROS production, MnSOD activation and periods of fasting and re-feeding in freshwater shrimp Neocaridina davidi (Crustacea, Malacostraca).
- Author
-
Włodarczyk A, Wilczek G, Wilczek P, Student S, Ostróżka A, Tarnawska M, and Rost-Roszkowska M
- Abstract
The middle region of the digestive system, the midgut of freshwater shrimp Neocaridina davidi is composed of a tube-shaped intestine and the hepatopancreas formed by numerous caeca. Two types of cells have been distinguished in the intestine, the digestive cells (D-cells) and regenerative cells (R-cells). The hepatopancreatic tubules have three distinct zones distinguished along the length of each tubule-the distal zone with R-cells, the medial zone with differentiating cells, and the proximal zone with F-cells (fibrillar cells) and B-cells (storage cells). Fasting causes activation of cell death, a reduction in the amount of reserve material, and changes in the mitochondrial membrane potential. However, here we present how the concentration of ROS changes according to different periods of fasting and whether re-feeding causes their decrease. In addition, the activation/deactivation of mitochondrial superoxide dismutase (MnSOD) was analyzed. The freshwater shrimps Neocaridina davidi (Crustacea, Malacostraca, Decapoda) were divided into experimental groups: animals starved for 14 days, animals re-fed for 4, 7, and 14 days. The material was examined using the confocal microscope and the flow cytometry. Our studies have shown that long-term starvation increases the concentration of free radicals and MnSOD concentration in the intestine and hepatopancreas, while return to feeding causes their decrease in both organs examined. Therefore, we concluded that a distinct relationship between MnSOD concentration, ROS activation, cell death activation and changes in the mitochondrial membrane potential occurred., Competing Interests: The authors declare there are no competing interests., (©2019 Włodarczyk et al.)
- Published
- 2019
- Full Text
- View/download PDF
35. Microevolution or wide tolerance? Level of stress proteins in the beet armyworm Spodoptera eqigua hübner (Lepidoptera: Noctuidae) exposed to cadmium for over 150 generations.
- Author
-
Tarnawska M, Kafel A, Augustyniak M, Rost-Roszkowska M, and Babczyńska A
- Subjects
- Animals, Antioxidants metabolism, Beta vulgaris growth & development, Cadmium metabolism, Larva drug effects, Larva metabolism, Models, Theoretical, Pupa drug effects, Pupa metabolism, Soil Pollutants metabolism, Spodoptera metabolism, Cadmium toxicity, Drug Tolerance, HSP70 Heat-Shock Proteins metabolism, Heat-Shock Proteins metabolism, Metallothionein metabolism, Soil Pollutants toxicity, Spodoptera drug effects
- Abstract
The aim of this study was to investigate whether the cadmium tolerance developed in the beet armyworm Spodoptera exigua selected for over 150 generations may be related to synthesis of the stress proteins metallothioneins (Mts) and 70 kDa heat shock proteins (HSP70). To achieve this, six S. exigua strains (control, k), 150-generation Cd exposure strain (cd), and four 18-generation Cd exposure strains differing in Cd concentration (cd44, cd22, cd11, cd5) were reared. Stress protein level was measured in the midgut of the 5th larval stage after 1-6, 12 and 18 generations. Cd contents was measured in the pupae. Unlike Cd concentration, which depended on metal contents in food but was not generation-dependent, the pattern of Mts and HSP70 concentrations changed in experimental strains from generation to generation. Stress protein levels in the insects exposed to the highest Cd concentration (the same as in the 150-generation Cd exposure strain), initially higher than in the control strain, after the 12th generation did not differ from the level measured in the control strains. It seems therefore that stress proteins play a protective role in insects of lower tolerance to cadmium. The tolerance developed during multigenerational exposure probably relies on mechanisms other than Mt and HSP70 synthesis., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
36. A pilot study with flow mediated skin fluorescence: A novel device to assess microvascular endothelial function in coronary artery disease.
- Author
-
Tarnawska M, Dorniak K, Kaszubowski M, Dudziak M, and Hellmann M
- Subjects
- Coronary Artery Disease diagnosis, Equipment Design, Female, Humans, Male, Middle Aged, Pilot Projects, Blood Flow Velocity physiology, Coronary Artery Disease physiopathology, Endothelium, Vascular physiopathology, Luminescent Measurements instrumentation, Microcirculation physiology, Skin blood supply, Vasodilation physiology
- Abstract
Background: Endothelial dysfunction is one of the earliest vascular manifestations in the pathogen-esis of cardiovascular disease. Noninvasive, simple, and inexpensive methods of endothelial function assessment are therefore needed., Methods: Microvascular endothelial function was assessed in coronary artery disease (CAD) patients by flow mediated skin fluorescence (FMSF), based on measurements of reduced form of nicotinamide adenine dinucleotide (NADH) fluorescence intensity during brachial artery occlusion (ischemic re-sponse [IRmax]) and immediately after occlusion (hyperemic response [HRmax]). Additionally, plasma levels of asymmetric dimethylarginine (ADMA) and endothelin-1 (ET-1) were measured to assess the association between biochemical markers and microvascular function evaluated in vivo by FMSF., Results: A significant inverse correlation was found between ADMA levels and hyperemic response (r = -0.534, p = 0.003), while ET-1 levels were inversely related to the ischemic response (r= -0.575, p = 0.001). Both IR and HR were found lowest in patients with advanced CAD and diabetes. When the repeatability of the method was tested, the intraclass correlation coefficient for IRmax and HRmax were 0.985 (p < 0.001) and 0.914 (p < 0.001), respectively. Moreover, in Bland and Altman analysis, both variables IRmax and HRmax showed good agreement in repeated measurements., Conclusions: In this pilot study, it was demonstrated that NADH fluorescence measured by FMSF device in CAD patients was associated with established plasma endothelial markers, and that both ischemic and hyperemic response were blunted in patients with advanced disease and diabetes. Fur-thermore, FMSF device showed excellent repeatability and good agreement for repeated measurements. However, further study is warranted to confirm these results in a larger patient cohort. (Cardiol J 2018; 25, 1: 120-127).
- Published
- 2018
- Full Text
- View/download PDF
37. Cross tolerance in beet armyworm: long-term selection by cadmium broadens tolerance to other stressors.
- Author
-
Augustyniak M, Tarnawska M, Babczyńska A, Kafel A, Zawisza-Raszka A, Adamek B, and Płachetka-Bożek A
- Subjects
- Animals, HSP70 Heat-Shock Proteins metabolism, Larva, Adaptation, Physiological physiology, Beta vulgaris physiology, Cadmium toxicity, Soil Pollutants toxicity, Spodoptera physiology
- Abstract
Long lasting exposure of animals to stressing factor may lead to the selection of population able to cope with the stressor at lower cost than unexposed individuals. The aim of this study was to assess whether 130-generational selection of a beet armyworm to cadmium in food might have induced tolerance also to other stressors. The potential tolerance was assessed by means of unspecific stress markers: HSP70 concentration, DNA damage level, and energy budget indices in L5 larval instars of beet armyworm. The animals originated from Cd-exposed and control strains exposed additionally in a short-term experiment to high/low temperature or pesticide-spinosad. The application of the additional stressors caused, in general, an increase in the levels of studied parameters, in a strain-dependent manner. The most significant increase was found in HSP70 level in the individuals from the Cd-strain exposed to various spinosad concentration. Therefore, multigenerational contact with cadmium caused several changes that enable the insect to survive under a chronic stress, preparing the organism to the contact with an additional, new stressor. This relationship may be described as a sort of cross tolerance. This may, possibly, increase the probability of population survivorship and, at the same time, decrease the efficiency of pesticide-based plant protection efforts.
- Published
- 2017
- Full Text
- View/download PDF
38. Reproducibility of flow mediated skin fluorescence to assess microvascular function.
- Author
-
Hellmann M, Tarnawska M, Dudziak M, Dorniak K, Roustit M, and Cracowski JL
- Subjects
- Adult, Biomarkers metabolism, Blood Flow Velocity, Case-Control Studies, Coronary Artery Disease metabolism, Coronary Artery Disease physiopathology, Feasibility Studies, Female, Humans, Hyperemia physiopathology, Ischemia physiopathology, Luminescent Measurements, Male, Middle Aged, Oxidation-Reduction, Predictive Value of Tests, Regional Blood Flow, Reproducibility of Results, Skin Temperature, Time Factors, Brachial Artery physiology, Coronary Artery Disease diagnosis, Microcirculation, NAD metabolism, Skin blood supply, Tourniquets, Upper Extremity blood supply
- Abstract
Objective: Recent technical developments enable skin fluorescence to be quantified in vivo in humans. The present study aimed at determining whether flow mediated skin fluorescence was reproducible, sensitive to changes within an individual, and if it could differ between patients with coronary artery disease and healthy volunteers., Methods: First, forearm flow mediated skin fluorescence recorded during and after brachial artery occlusion was assessed following successive forearm occlusion periods (1, 2, 3 and 5min) and expressed as ischemic and hyperemic responses (as % of baseline). Secondly, 3min flow mediated skin fluorescence was assessed before and after 10min local cooling to 15°C. In a third protocol, the inter-day reproducibility of ischemic and hyperemic responses to 3min occlusion was tested at an interval of 7days, and compared between healthy controls and patients with coronary artery disease (CAD)., Results: In the first protocol, we observed a time dependent increase in the ischemic and hyperemic responses to occlusion. Next, we observed a lower hyperemic response after local cooling (9.8±4.2 versus 17.8±2.5% respectively, P<0.001), while in contrast, the ischemic response was higher and exhibited greater variability (23±15 versus 11.8±6.4%; P=0.028). In the third protocol, the inter-day reproducibility of flow mediated skin fluorescence for a 3min occlusion period was excellent. The ischemic response was significantly lower in CAD patients than in healthy controls (6.7±4.8% vs 14.7±6.8% respectively, P<0.001). Similarly, the hyperemic response was significantly decreased in the CAD group compared to healthy controls (11.6±3.6% vs 19.5±5.4% respectively, P<0.001)., Conclusion: We show that quantifying the ischemic and hyperemic flow mediated skin fluorescence is feasible, reproducible, sensitive to acute changes in skin blood flow, and distinguishes patients populations. However, more data are needed to evaluate the correlation with other methods or specific biochemical endothelial markers., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
39. Phenotypic Plasticity, Epigenetic or Genetic Modifications in Relation to the Duration of Cd-Exposure within a Microevolution Time Range in the Beet Armyworm.
- Author
-
Augustyniak M, Płachetka-Bożek A, Kafel A, Babczyńska A, Tarnawska M, Janiak A, Loba A, Dziewięcka M, Karpeta-Kaczmarek J, and Zawisza-Raszka A
- Subjects
- Animals, Apoptosis drug effects, Beta vulgaris parasitology, Epigenesis, Genetic drug effects, Hemocytes drug effects, Humans, Hydrogen Peroxide pharmacology, Insecta genetics, Larva drug effects, Larva genetics, Oxidative Stress drug effects, Biological Evolution, Cadmium toxicity, DNA Damage drug effects, Environmental Pollutants toxicity, Insecta drug effects
- Abstract
In the case of the pests inhabiting metal polluted or fields where the use of pesticides is common, a natural selection of resistant individuals can occur. This may pose serious problems for humans, agriculture, as well as the economies of many countries. In this study, the hypothesis that multigenerational (120 generations) exposure to cadmium of a beet armyworm population could be a selecting factor toward a more efficient DNA protection was verified. The hemocytes of individuals from two culture strains (control and Cd-exposed) were treated with H2O2 (a DNA-damaging agent) or PBS (reference). The level of DNA damage was assessed using the Comet assay immediately and 5, 15 and 30 min. after the treatment. The immediate result of the contact with H2O2 was that the level of DNA damage in the hemocytes of the insects from both strains increased significantly. However, in the cells of the Cd-exposed individuals, the level of DNA damage decreased over time, while in the cells from the control insects it remained at the same level with no evidence of repair. These results suggest that efficient defense mechanisms may exist in the cells of insects that have prolonged contact with cadmium. Some evolutionary and trade-off aspects of the phenomenon are discussed. In a wider context, comparing the results obtained in the laboratory with field studies may be beneficial for understanding basic mechanisms of the resistance of an organism. To summarize, the high potential for the repair of DNA damage that was observed in the insects from the cadmium strain may confirm the hypothesis that multigenerational exposure to that metal may possibly contribute to the selection of insects that have a wider tolerance to oxidative stress. However, our investigations of polymorphism using AFLP did not reveal differences between the two main insect strains., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2016
- Full Text
- View/download PDF
40. Elemental distribution in reproductive and neural organs of the Epilachna nylanderi (Coleoptera: Coccinellidae), a phytophage of nickel hyperaccumulator Berkheya coddii (Asterales: Asteraceae) by micro-PIXE.
- Author
-
Mesjasz-Przybyłowicz J, Orłowska E, Augustyniak M, Nakonieczny M, Tarnawska M, Przybyłowicz W, and Migula P
- Subjects
- Animals, Central Nervous System metabolism, Genitalia metabolism, Male, Spectrometry, X-Ray Emission, Asteraceae, Coleoptera metabolism, Herbivory, Micronutrients metabolism, Nickel metabolism
- Abstract
The phenomenon of metal hyperaccumulation by plants is often explained by a pathogen or herbivore defense hypothesis. However, some insects feeding on metal hyperaccumulating plants are adapted to the high level of metals in plant tissues. Former studies on species that feed on the leaves of Berkheya coddii Roessler 1958 (Asteraceae), a nickel-hyperaccumulating plant, demonstrated several protective mechanisms involved in internal distribution, immobilization, and elimination of Ni from the midgut and Malpighian tubules. These species are mainly coleopterans, including the lady beetle, Epilachna nylanderi (Mulsant 1850) (Coleoptera: Coccinellidae), collected from the ultramafic ecosystem near Barberton in South Africa. By performing particle-induced X-ray emission microanalysis elemental microanalysis (PIXE), this study examined whether Ni may be harmful to internal body systems that decide on insect reactivity (central nervous system [CNS]), their reproduction, and the relationships between Ni and other micronutrients. Data on elemental distribution of nine selected elements in target organs of E. nylanderi were compared with the existing data for other insect species adapted to the excess of metals. Micro-PIXE maps of seven regions of the CNS showed Ni mainly in the neural connectives, while cerebral ganglia were better protected. Concentrations of other bivalent metals were lower than those of Ni. Testis, compared with other reproductive organs, showed low amounts of Ni. Zn was effectively regulated at physiological dietary levels. In insects exposed to excess dietary Zn, it was also accumulated in the reproductive organs. Comparison of E. nylanderii with other insects that ingest hyperaccumulating plants, especially chrysomelid Chrysolina clathrata (Clark) (Coleoptera: Chrysomelidae), showed lower protection of the CNS and reproductive organs., (© The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America.)
- Published
- 2014
- Full Text
- View/download PDF
41. Hsp70 level in progeny of aging grasshoppers from variously polluted habitats and additionally exposed to zinc during diapause.
- Author
-
Augustyniak M, Tarnawska M, Babczyńska A, and Augustyniak M
- Subjects
- Animals, Ecosystem, Female, Gene Expression drug effects, Grasshoppers genetics, Grasshoppers physiology, HSP70 Heat-Shock Proteins genetics, Insect Proteins genetics, Metamorphosis, Biological drug effects, Oviposition drug effects, Environmental Pollutants toxicity, Grasshoppers drug effects, Grasshoppers growth & development, HSP70 Heat-Shock Proteins metabolism, Insect Proteins metabolism, Zinc pharmacology
- Abstract
The hsp70 level in the bodies of 1st instars of grasshoppers Chorthippus brunneus from unpolluted (Pilica) and polluted (Olkusz, Szopienice) sites and additionally exposed to various doses of zinc during diapause and embryonic development prior to hatching were measured by Western blotting. The main aim of our work was to assess the relationship between the age of female grasshoppers originating from variously polluted habitat and the hsp70 level in their progeny. Possible reasons for population variation in hsp70 levels were discussed. The hsp70 level in the offspring's body depended on the place of origin. The strongest expression of hsp70 was found in the bodies of larvae hatching from the eggs laid by young females from Pilica (reference site). In contrast, a low initial level of hsp70 in larvae from polluted sites, especially in young females' progeny, was observed. The application of zinc during diapause influenced the hsp70 level in grasshopper larvae; however, the direction of the changes depended on the insects' place of origin. In larvae from the reference site, and also (but to a lesser degree) from Olkusz, the increase in the hsp70 level after zinc treatment was most pronounced. Whereas in grasshoppers from Szopienice, zinc (in 100microg g(-1) dry weight of sand) did not change the hsp70 level, or (in 500microg g(-1) dry weight of sand) caused a reduction in hsp70. The differences may result from maternal effects; however, possible adaptation also cannot be excluded. To confirm this statement further studies are needed.
- Published
- 2009
- Full Text
- View/download PDF
42. [Late episodes of apnea in a premature infant infected with E. coli O111K58].
- Author
-
Maszkiewicz W, Tarnawska M, and Benysek F
- Subjects
- Humans, Infant, Newborn, Male, Apnea etiology, Escherichia coli Infections complications, Infant, Premature, Diseases
- Abstract
Typical episodes of apnoea with paleness and cyanosis have been noted noted in premature baby born on the 28th week of pregnancy with body weight 1,010 g as a result of infection with enteropathogenic strain of E. coli O111K58 on the 21st day of life (3rd day of the infection). Effective treatment with antibiotics produced recovery.
- Published
- 1992
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.