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Background: As increase of cytokines (like IL-1 , IL-6 , IL-8 and TNF- ) in platelet concentrates (PCs) during storage are involved in Febrile Non Hemolytic Transfusion Reactions (FNHTRs) after Platelet transfusion, the aim of this study was the survey of these cytokines level in PCs produced in Tehran Blood Center. Materials and Methods: This study proposed to determine if WBC reduction in PCs by pre-storage leuckodepletion filters reduced the levels of these cytokines during storage uo 3 days. Each of the PCs (n = 54) was prepared from single random donor (RD) by Platelet-Rich-Plasma (PRP), then were divided in four groups: 1- unfiltered non-irradiated RD-PCs (n= 13) 2- unfiltered and -irradiated RD-PCs (n=16) 3 - filtered non-irradiated RD-PCs (n=14) 4-filtered and -irradiated RD-PCs (n = 11). Cytokines levels in PCs supernatants were measured by ELISA kits according manufacture‘s instructions. Results: Our results showed : IL-8 was increased in unfiltered non-irradiated and IL-18 in -irradiated RD-PCs but not in the filtered RD-PCs in day 3 . Compared to unfiltered PCs in filtered units, pre-storage filtration prevented a rise in the IL-8 and TNF-  in day 3 of storage .The concentration of IL-1 was lower than the minimal detectable concentration by the kits used for this purpose. IL-6 was detected only in 7 units of all filtered PCs in day 3. Conclusion: These data indicate that pre-storage leuko-reduction of PCs can prevent accumulation of IL-6, IL-8 and TNF-  during storage.

CMV belongs to herpes viridea family and it is the largest human virus. Prevalence of CMV depends on age, race, geographic and socioeconomic factors. CMV infection has been a recognized complication of transfusion for about three decades, in an immature or compromised immune system situation. If a transplant candidate has not been infected with CMV and no CMV specific antibodies can be detected by serology, a primary infection could be transmitted via transfusion or transplantation. Patient who are under dialysis are susceptible to CMV infection, in addition of increase serum levels of p2-MG(Beta-2 Microglobulin)."nMaterials and Methods: we detected anti CMV (IgM -IgG) antibodies for 128 renal transplant candidates who were under intermittent heamodialysis and 1040 blood donors, as controls and compared serum p2-MG levels in 48 of the patients with 35 controls with ELISA technique. For 15 patients, R5 (Cuprophan) and for 7 patients S2 (Polysulphone) filters were used."nResults: Our data showed:"n1-90 percent of normal blood donors and 89.8 percent of the patients were IgG positive. Statistical analysis showed no significant difference between these two groups (pO.Ol). This reflected high prevalence of CMV ."n2- 0.2 percent of normal individuals and 2.3 percent of the patients were positive for IgM. There was significant difference in IgM between these two groups (p