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The complete amino acid sequence of the short subfragment- 2 (short S-2) from adult chicken gizzard muscle has been determined by direct analysis of peptides derived from digests of this portion with cyanogen bromide, lysyl endopeptidase and from hydrolysates of CNBr fragments with dilute formic acid. It contains 323 amino acid residues which span the NH2-terminus of the subfragment-2 (S-2) to the NH2 -terminal portion of the hinge region in S-2. Although, structural differences between embryonic and adult chicken gizzard muscle myosin heavy chain (MHC) have been suggested, comparison of the amino acid sequence of the short S-2 from adult chicken gizzard muscle MHC with that from embryonic chicken gizzard MHC, deduced from its nucleotide sequence of the cDNA, showed that the sequence were identical. The amino acid sequence of the short S-2 from adult chicken gizzard muscle MHC was compared with the corresponding sequence of chicken brain myosin, Drosophila non-muscle myosin, chicken skeletal muscle myosin and chicken cardiac ventricular muscle myosin. The results show relatively higher sequence identities with chicken brain myosin (76.5%) and Drosophila non-muscle myosin (60.0%) and lower identities with chicken skeletal muscle myosin (36.8%) and chicken cardiac ventricular muscle myosin (36.5%)., Acta medica Nagasakiensia. 1996, 41(1-2), p.58-66

われわれは ［Watanabe B （1989），Biol. Chem. Hoppe-Seyler 370 （9）: 1027-1034 and Watanabe B, Tanigawa M （1993）, Biol. Chem. Hoppe-Seyler 374 （1）: 27-35］において成鶏の骨格筋，心室筋ミオシンのサブフラグメント‐2の構造と機能の関連性について発表したが，今回の論文は成鶏砂のう筋ミオシン（平滑筋）のショート・サブフラグメント‐2とヒンジとの連結部領域の構造を明らかにしたものである。 ミオシンをパパインで消化して得られた尾部（ロッド）をさらにα‐キモトリプシンで限定消化を行い，サブフラグメント‐2（S-2）とライトメロミオシン（LMM）に分離した。次いでサブフラグメント‐2のブロムシアン分解ペプチドの中から，ショート・サブフラグメント‐2のカルボキシ末端領域とヒンジのアミノ末端領域を含む134個のアミノ酸からなるペプチドを精製単離し，一次構造を決定した． さらに構造的相異を分析するために上記のようにして決定した連結部分の構造を胎鶏の砂のう筋（平滑筋），成鶏の脳，ドロソフィラ，成鶏骨格筋，成鶏心室筋ミオシンの相同部分の構造と比較した．その結果，興味あることに胎鶏の砂のう筋，成鶏の脳，ドロソフィラの非筋ミオシンとはそれぞれ100％，81.3％，59.7％の高いアミノ酸の同一性が認められ，これに対し成鶏骨格筋と成鶏心室筋ミオシンとはそれぞれ38.8％，40.3％と低いアミノ酸の同一性が認められた．, In the preceding papers [Watanabe B (1989), Biol. Chem. Hoppe-Seyler 370 (9): 1027-1034 and Watanabe B, Tanigawa M (1993), Biol. Chem. Hoppe-Seyler 374 (1): 27-35] we reported primary structures of subfragment-2 (S-2) from adult chicken skeletal and cardiac muscle myosins. This paper describes the structure of the junctional region between the short subfragment-2 (short S-2) and the hinge from adult chicken gizzard muscle myosin. The rod obtained by digesting myosin with papain was further subdivided into S-2 and light meromyosin (LMM) by limited digestion with α-chymotrypsin. A 134 amino-acid-residue peptide covered with carboxy-terminal portion of the short S-2 and amino-terminal portion of the hinge was isolated by conventional method from cyanogen bromide (CNBr) digests of S-2 and sequenced. To analyse the structural differences, the sequence of this junction thus determined was compared with those of corresponding portions of embryonic chicken gizzard, chicken brain, drosophila, chicken skeletal and chicken cardiac muscle myosin. Interestingly, results show the higher sequence identities of 100%, 81.3% and 59.7% with embryonic chicken gizzard, chicken brain and drosophila nonmuscle myosin, respectively, and lower degrees, 38.8% and 40.3% with chicken skeletal and chicken cardiac ventricular muscle myosin respectively., 長崎大学医療技術短期大学部紀要 = Bulletin of the School of Allied Medical Sciences, Nagasaki University. 1995, 8, p.1-8

長崎大学小児科未熟児室で集中管理を受けた在胎28週から35週のAFD16例を研究対象とした。 NBAS評価は36、40、44週の計3回施行し、6カ月、12カ月時点での発達評価はBAYLEY乳幼児発達検査を実施し、併せて、生活環境調査もおこなった。 1、睡眠のリズムと生活環境が1才時点での運動および精神発達指数に大きく影響していた。2、明らかな発達遅滞児2例、脳性麻痺1例を除いた1才時点での発達指数は、運動102.8±7.4、精神105.6±6.1と標準値に達しており、NBASを用いた早期介入がAFDの発達促進に有効であったと推測される。3、発達遅滞児、脳性麻痺児はNBAS補足項目と反射項目で低値を示し、high risk児の評価に有効であった。, It has constantly been observe that cerebral palsy and/or mental retardation develops among premature and high risk infants ．Assessment using Neonatal Behavioral Assessment Scale （NBAS）immediately after birth and longitudinal study on development during the first postnatal year were performed on 16 AFD （appropriate for dates ）infants whose normal development was anticipated and 21 mature infants, in order to improve the security of early assessment and early habilitation of handicapped children． NBAS assessment was effective as a means of early intervention ，whereby development Was enhanced in both AFD and mature infants. Of the 13 AFD infants who reached one year of age 1 case was hemiplegic cerebral palsy and 2 cases were motor delay. They showed abnormal values in NBAS supplementary items and reflexes at the neonatal period ．These itmes were Effective for a screening test of high risk infants. The mental development index （MDI） and psychomotor development index （PDI） were closely associate with the quality of sleep-waking ryhthm and the degree of mother's interaction．, 長崎大学医療技術短期大学部紀要 = Bulletin of the School of Allied Medical Sciences, Nagasaki University. 1990, 3, p.1-12