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49 results on '"Tanganuchitcharnchai, Ampai"'

10. Scrub typhus in Indonesia: A cross-sectional analysis of archived fever studies samples.

Author

Saraswati, Kartika, Tanganuchitcharnchai, Ampai, Ongchaikupt, Sirada, Mukaka, Mavuto, Day, Nicholas P J, Baird, J Kevin, Antonjaya, Ungke, Myint, Khin S A, Dewi, Yora P, Yudhaputri, Frilasita A, Haryanto, Sotianingsih, Witari, N P Diah, and Blacksell, Stuart D

Subjects
TSUTSUGAMUSHI disease, CROSS-sectional method, ENZYME-linked immunosorbent assay, BACTERIAL diseases, BETA lactam antibiotics, FEVER
Abstract

Background Scrub typhus is an understudied vector-borne bacterial infection. Methods We tested archived fever samples for scrub typhus seropositivity to begin charting its geographic distribution in Indonesia. We analysed 1033 serum samples from three sites. IgM and IgG enzyme-linked immunosorbent assay (ELISA) against Orientia tsutsugamushi was performed using Karp, Kato, Gilliam, TA 716 antigens. To determine the cutoff in the absence of a presumed unexposed population and gold standard tests, we identified the visual inflection point, performed change point analysis, and used finite mixture models. Results The optical density cutoff values used for IgM and IgG were 0.49 and 0.13, respectively. Across all sites, IgM seropositivity was 4.6% (95% CI: 3.4 to 6.0%) while IgG seropositivity was 4.4% (95% CI: 3.3 to 5.8%). The overall seropositivity across sites was 8.8% (95% CI: 8.1 to 11.7%). The overall seropositivity for Jambi, Denpasar, Tabanan were 9.7% (95% CI: 7.0 to 13.3%), 8.0% (95% CI: 5.7 to 11.0%), 9.0% (95% CI: 6.1 to 13.0%), respectively. Conclusions We conclude that O. tsutsugamushi exposure in humans occurred at all sites analysed and could be the cause of illness in some cases. Though it was not the main cause of acute fever in these locations, it is still important to consider scrub typhus in cases not responding to beta-lactam antibiotics. Future seroprevalence surveys and testing for scrub typhus in acute febrile illness studies will be essential to understand its distribution and burden in Indonesia. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Serosurvey of Scrub Typhus in Indonesia: A Cross-Sectional Analysis of Archived Fever Studies Samples

Author

Saraswati, Kartika, primary, Tanganuchitcharnchai, Ampai, additional, Ongchaikupt, Sirada, additional, Mukaka, Mavuto, additional, Day, Nicholas P. J., additional, Baird, J. Kevin, additional, Jaya, Ungke Anton, additional, Myint, Khin S. A., additional, Dewi, Yora Permata, additional, Yudhaputri, Frilasita A., additional, Haryanto, Sotianingsih, additional, Witari, Ni P. D., additional, and Blacksell, Stuart D., additional

Published
2022
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16. Diagnostic accuracy of an in-house Scrub Typhus enzyme linked immunoassay for the detection of IgM and IgG antibodies in Laos

Author

Elders, Philip N. D., primary, Dhawan, Sandhya, additional, Tanganuchitcharnchai, Ampai, additional, Phommasone, Koukeo, additional, Chansamouth, Vilada, additional, Day, Nicholas P. J., additional, Garcia-Rivera, Jose A., additional, Hertz, Jeffrey C., additional, Mayxay, Mayfong, additional, Vongsouvath, Manivanh, additional, Dubot-Pérès, Audrey, additional, Robinson, Matthew T., additional, Newton, Paul N., additional, and Blacksell, Stuart D., additional

Published
2020
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17. Determination of Optimal Diagnostic Cut-Offs for the Naval Medical Research Center Scrub Typhus IgM ELISA in Chiang Rai, Thailand

Author

Phanichkrivalkosil, Meghna, primary, Tanganuchitcharnchai, Ampai, additional, Jintaworn, Suthatip, additional, Kantipong, Pacharee, additional, Laongnualpanich, Achara, additional, Chierakul, Wirongrong, additional, Paris, Daniel H., additional, Richards, Allen L., additional, Wangrangsimakul, Tri, additional, Day, Nicholas P. J., additional, and Blacksell, Stuart D., additional

Published
2019
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19. Rickettsial Illnesses as Important Causes of Febrile Illness in Chittagong, Bangladesh

Author

Kingston, Hugh W., primary, Hossain, Mosharraf, additional, Leopold, Stije, additional, Anantatat, Tippawan, additional, Tanganuchitcharnchai, Ampai, additional, Sinha, Ipsita, additional, Plewes, Katherine, additional, Maude, Richard J., additional, Chowdhury, M.A. Hassan, additional, Paul, Sujat, additional, Uddin, Rabiul Alam Mohammed Erfan, additional, Siddiqui, Mohammed Abu Naser, additional, Zahed, Abu Shahed, additional, Abu Sayeed, Abdullah, additional, Rahman, Mohammed Habibur, additional, Barua, Anupam, additional, Uddin, Mohammed Jasim, additional, Sattar, Mohammed Abdus, additional, Dondorp, Arjen M., additional, Blacksell, Stuart D., additional, Day, Nicholas P.J., additional, Ghose, Aniruddha, additional, Hossain, Amir, additional, and Paris, Daniel H., additional

Published
2018
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21. Strong interferon-gamma mediated cellular immunity to scrub typhus demonstrated using a novel whole cell antigen ELISpot assay in rhesus macaques and humans

Author

Sumonwiriya, Manutsanun, primary, Paris, Daniel H., additional, Sunyakumthorn, Piyanate, additional, Anantatat, Tippawan, additional, Jenjaroen, Kemajittra, additional, Chumseng, Suchintana, additional, Im-erbsin, Rawiwan, additional, Tanganuchitcharnchai, Ampai, additional, Jintaworn, Suthatip, additional, Blacksell, Stuart D., additional, Chowdhury, Fazle R., additional, Kronsteiner, Barbara, additional, Teparrukkul, Prapit, additional, Burke, Robin L., additional, Lombardini, Eric D., additional, Richards, Allen L., additional, Mason, Carl J., additional, Jones, James W., additional, Day, Nicholas P. J., additional, and Dunachie, Susanna J., additional

Published
2017
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22. Optimal Cutoff and Accuracy of an IgM Enzyme-Linked Immunosorbent Assay for Diagnosis of Acute Scrub Typhus in Northern Thailand: an Alternative Reference Method to the IgM Immunofluorescence Assay

Author

Blacksell, Stuart D., primary, Lim, Cherry, additional, Tanganuchitcharnchai, Ampai, additional, Jintaworn, Suthatip, additional, Kantipong, Pacharee, additional, Richards, Allen L., additional, Paris, Daniel H., additional, Limmathurotsakul, Direk, additional, and Day, Nicholas P. J., additional

Published
2016
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25. Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology

Author

Kingston, Hugh W. F., Blacksell, Stuart D., Tanganuchitcharnchai, Ampai, Laongnualpanich, Achara, Basnyat, Buddha, Day, Nicholas P. J., Paris, Daniel H., Kingston, Hugh W. F., Blacksell, Stuart D., Tanganuchitcharnchai, Ampai, Laongnualpanich, Achara, Basnyat, Buddha, Day, Nicholas P. J., and Paris, Daniel H.

Abstract

This study investigated the comparative accuracy of a recombinant 56-kDa type-specific antigen-based rapid diagnostic test (RDT) for scrub typhus for the detection of IgM antibodies by using conventional serology in well-characterized serum samples from undifferentiated febrile illness patients. The RDT showed high specificity and promising comparative accuracy, with 82% sensitivity and 98% specificity for samples defined positive at an IgM indirect immunofluorescence assay positivity cutoff titer of ≥1:1,600 versus 92% and 95% at ≥1:6,400, respectively.

Published
2015

26. Diagnostic Accuracy Assessment of Immunochromatographic Tests for the Rapid Detection of Antibodies Against Orientia tsutsugamushi Using Paired Acute and Convalescent Specimens

Author

Watthanaworawit, Wanitda, primary, Turner, Paul, additional, Turner, Claudia, additional, Tanganuchitcharnchai, Ampai, additional, Jintaworn, Suthatip, additional, Hanboonkunupakarn, Borimas, additional, Richards, Allen L., additional, Day, Nicholas P. J., additional, Blacksell, Stuart D., additional, and Nosten, François, additional

Published
2015
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27. Performance of C-reactive protein and procalcitonin to distinguish viral from bacterial and malarial causes of fever in Southeast Asia

Author

Lubell, Yoel, primary, Blacksell, Stuart D., additional, Dunachie, Susanna, additional, Tanganuchitcharnchai, Ampai, additional, Althaus, Thomas, additional, Watthanaworawit, Wanitda, additional, Paris, Daniel H., additional, Mayxay, Mayfong, additional, Peto, Thomas J., additional, Dondorp, Arjen M., additional, White, Nicholas J., additional, Day, Nicholas P.J., additional, Nosten, François, additional, Newton, Paul N., additional, and Turner, Paul, additional

Published
2015
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29. Underrecognized Arthropod-Borne and Zoonotic Pathogens in Northern and Northwestern Thailand: Serological Evidence and Opportunities for Awareness

Author

Blacksell, Stuart D., primary, Kantipong, Pacharee, additional, Watthanaworawit, Wanitda, additional, Turner, Claudia, additional, Tanganuchitcharnchai, Ampai, additional, Jintawon, Sutathip, additional, Laongnuanutit, Achara, additional, Nosten, François H., additional, Day, Nicholas P.J., additional, Paris, Daniel H., additional, and Richards, Allen L., additional

Published
2015
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30. Pregnancy Outcome in Relation to Treatment of Murine Typhus and Scrub Typhus Infection: A Fever Cohort and a Case Series Analysis

Author

McGready, Rose, primary, Prakash, John Antony Jude, additional, Benjamin, Santosh Joseph, additional, Watthanaworawit, Wanitda, additional, Anantatat, Tippawan, additional, Tanganuchitcharnchai, Ampai, additional, Ling, Clare L., additional, Tan, Saw Oo, additional, Ashley, Elizabeth A., additional, Pimanpanarak, Mupawjay, additional, Blacksell, Stuart D., additional, Day, Nicholas P., additional, Singhasivanon, Pratap, additional, White, Nicholas J., additional, Nosten, François, additional, and Paris, Daniel H., additional

Published
2014
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31. Serosurveillance of Orientia tsutsugamushi and Rickettsia typhi in Bangladesh

Author

Maude, Rapeephan R., primary, Maude, Richard J., additional, Ghose, Aniruddha, additional, Amin, M. Robed, additional, Islam, M. Belalul, additional, Ali, Mohammad, additional, Bari, M. Shafiqul, additional, Majumder, M. Ishaque, additional, Tanganuchitcharnchai, Ampai, additional, Dondorp, Arjen M., additional, Paris, Daniel H., additional, Bailey, Robin L., additional, Faiz, M. Abul, additional, Blacksell, Stuart D., additional, and Day, Nicholas P. J., additional

Published
2014
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32. A Prospective Evaluation of Real-Time PCR Assays for the Detection of Orientia tsutsugamushi and Rickettsia spp. for Early Diagnosis of Rickettsial Infections during the Acute Phase of Undifferentiated Febrile Illness

Author

Watthanaworawit, Wanitda, primary, Turner, Paul, additional, Turner, Claudia, additional, Tanganuchitcharnchai, Ampai, additional, Richards, Allen L., additional, Bourzac, Kevin M., additional, Blacksell, Stuart D., additional, and Nosten, François, additional

Published
2013
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34. Comparison of Seven Commercial Antigen and Antibody Enzyme-Linked Immunosorbent Assays for Detection of Acute Dengue Infection

Author

Blacksell, Stuart D., primary, Jarman, Richard G., additional, Gibbons, Robert V., additional, Tanganuchitcharnchai, Ampai, additional, Mammen, Mammen P., additional, Nisalak, Ananda, additional, Kalayanarooj, Siripen, additional, Bailey, Mark S., additional, Premaratna, Ranjan, additional, de Silva, H. Janaka, additional, Day, Nicholas P. J., additional, and Lalloo, David G., additional

Published
2012
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35. Orientia tsutsugamushi in Human Scrub Typhus Eschars Shows Tropism for Dendritic Cells and Monocytes Rather than Endothelium

Author

Paris, Daniel H., primary, Phetsouvanh, Rattanaphone, additional, Tanganuchitcharnchai, Ampai, additional, Jones, Margaret, additional, Jenjaroen, Kemajittra, additional, Vongsouvath, Manivanh, additional, Ferguson, David P. J., additional, Blacksell, Stuart D., additional, Newton, Paul N., additional, Day, Nicholas P. J., additional, and Turner, Gareth D. H., additional

Published
2012
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36. Evaluation of Six Commercial Point-of-Care Tests for Diagnosis of Acute Dengue Infections: the Need for Combining NS1 Antigen and IgM/IgG Antibody Detection To Achieve Acceptable Levels of Accuracy

Author

Blacksell, Stuart D., primary, Jarman, Richard G., additional, Bailey, Mark S., additional, Tanganuchitcharnchai, Ampai, additional, Jenjaroen, Kemajittra, additional, Gibbons, Robert V., additional, Paris, Daniel H., additional, Premaratna, Ranjan, additional, de Silva, H. Janaka, additional, Lalloo, David G., additional, and Day, Nicholas P. J., additional

Published
2011
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40. Diagnostic Accuracy of the InBios Scrub Typhus Detect Enzyme-Linked Immunoassay for the Detection of IgM Antibodies in Northern Thailand

Author

Blacksell, Stuart D., Tanganuchitcharnchai, Ampai, Nawtaisong, Pruksa, Kantipong, Pacharee, Laongnualpanich, Achara, Day, Nicholas P. J., and Paris, Daniel H.

Abstract

ABSTRACTIn this study, we examined the diagnostic accuracy of the InBios Scrub Typhus Detect IgM enzyme-linked immunosorbent assay (ELISA) and determined the optimal diagnostic optical density (OD) cutoffs for screening and diagnostic applications based on prospectively collected, characterized samples from undifferentiated febrile illness patients in northern Thailand. Direct comparisons with the serological gold standard, indirect immunofluorescence assay (IFA), revealed strong statistical correlation of ELISA OD values and IFA IgM titers. Determination of the optimal ELISA cutoff for seroepidemiology or screening purposes compared to the corresponding IFA reciprocal titer of 400 as previously described for Thailand was 0.60 OD, which corresponded to a sensitivity (Sn) of 84% and a specificity (Sp) of 98%. The diagnostic performance against the improved and more-stringent scrub typhus infection criteria (STIC), correcting for low false-positive IFA titers, resulted in an Sn of 93% and an Sp of 91% at an ELISA cutoff of 0.5 OD. This diagnostic ELISA cutoff corresponds to IFA reciprocal titers of 1,600 to 3,200, which greatly reduces the false-positive rates associated with low-positive IFA titers. These data are in congruence with the recently improved serodiagnostic positivity criteria using the Bayesian latent class modeling approach. In summary, the InBios Scrub Typhus Detect IgM ELISA is affordable and easy-to-use, with adequate diagnostic accuracy for screening and diagnostic purposes, and should be considered an improved alternative to the gold standard IFA for acute diagnosis. For broader application, regional cutoff validation and antigenic composition for consistent diagnostic accuracy should be considered.

Published
2015
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43. Strong interferon-gamma mediated cellular immunity to scrub typhus demonstrated using a novel whole cell antigen ELISpot assay in rhesus macaques and humans

Author

Sumonwiriya, Manutsanun, Paris, Daniel H., Sunyakumthorn, Piyanate, Anantatat, Tippawan, Jenjaroen, Kemajittra, Chumseng, Suchintana, Im-Erbsin, Rawiwan, Tanganuchitcharnchai, Ampai, Jintaworn, Suthatip, Blacksell, Stuart D., Chowdhury, Fazle R., Kronsteiner-Dobramysl, Barbara, Teparrukkul, Prapit, Burke, Robin L., Lombardini, Eric D., Richards, Allen L., Mason, Carl J., Jones, James W., Day, Nicholas P. J., and Dunachie, Susanna J.

Subjects
3. Good health

45. Rickettsial illnesses as important causes of febrile illness in Chittagong, Bangladesh

Author

Kingston, Hugh W., Hossain, Mosharraf, Leopold, Stije, Anantatat, Tippawan, Tanganuchitcharnchai, Ampai, Sinha, Ipsita, Plewes, Katherine, Maude, Richard J., Chowdhury, M. A. Hassan, Paul, Sujat, Uddin, Rabiul Alam Mohammed Erfan, Siddiqui, Mohammed Abu Naser, Zahed, Abu Shahed, Abu Sayeed, Abdullah, Rahman, Mohammed Habibur, Barua, Anupam, Uddin, Mohammed Jasim, Sattar, Mohammed Abdus, Dondorp, Arjen M., Blacksell, Stuart D., Day, Nicholas P. J., Ghose, Aniruddha, Hossain, Amir, and Paris, Daniel H.

Subjects
3. Good health

46. Antigenic Relationships among Human Pathogenic Orientia tsutsugamushi Isolates from Thailand

Author

James, Sarah L, Blacksell, Stuart D, Nawtaisong, Pruksa, Tanganuchitcharnchai, Ampai, Smith, Derek J, Day, Nicholas PJ, and Paris, Daniel H

Subjects
Orientia tsutsugamushi, Antigens, Bacterial, Genotype, Scrub Typhus, Genetic Variation, Humans, Cross Reactions, bacterial infections and mycoses, Thailand, Antigenic Variation, Phylogeny, 3. Good health
Abstract

BACKGROUND: Scrub typhus is a common cause of undiagnosed febrile illness in certain tropical regions, but can be easily treated with antibiotics. The causative agent, Orientia tsutsugamushi, is antigenically variable which complicates diagnosis and efforts towards vaccine development. METHODOLOGY/PRINCIPAL FINDINGS: This study aimed to dissect the antigenic and genetic relatedness of O. tsutsugamushi strains and investigate sero-diagnostic reactivities by titrating individual patient sera against their O. tsutsugamushi isolates (whole-cell antigen preparation), in homologous and heterologous serum-isolate pairs from the same endemic region in NE Thailand. The indirect immunofluorescence assay was used to titrate Orientia tsutsugamushi isolates and human sera, and a mathematical technique, antigenic cartography, was applied to these data to visualise the antigenic differences and cross-reactivity between strains and sera. No functional or antigen-specific analyses were performed. The antigenic variation found in clinical isolates was much less pronounced than the genetic differences found in the 56kDa type-specific antigen genes. The Karp-like sera were more broadly reactive than the Gilliam-like sera. CONCLUSIONS/SIGNIFICANCE: Antigenic cartography worked well with scrub typhus indirect immunofluorescence titres. The data from humoral responses suggest that a Karp-like strain would provide broader antibody cross-reactivity than a Gilliam-like strain. Although previous exposure to O. tsutsugamushi could not be ruled out, scrub typhus patient serum antibody responses were characterised by strong homologous, but weak heterologous antibody titres, with little evidence for cross-reactivity by Gilliam-like sera, but a broader response from some Karp-like sera. This work highlights the importance of antigenic variation in O. tsutsugamushi diagnosis and determination of new serotypes.

48. Orientia tsutsugamushi in Chiggers and Small Mammals in Laos.

Author

Elliott I, Kumlert R, Thangnimitchok N, Blacksell SD, Tanganuchitcharnchai A, Paris DH, Newton PN, and Morand S

Subjects
Animals, Laos epidemiology, Rodentia, Mammals, Immunoglobulin G, Orientia tsutsugamushi genetics, Trombiculidae, Scrub Typhus epidemiology, Scrub Typhus veterinary, Rodent Diseases epidemiology
Abstract

Background: Scrub typhus is a leading cause of febrile illness in Laos and accounts for a high burden of disease. There have been no previous studies on the causative agent, Orientia tsutsugamushi , in vector mites ("chiggers") or their small mammal hosts in Laos. Materials and Methods: Small mammals and free-living chiggers were trapped in districts of Vientiane Province and Capital. Tissues were tested for O. tsutsugamushi by PCR and serum for IgG to O. tsutsugamushi by immunofluorescence assays (IFAs). Chiggers removed from small mammals and collected in their free-living stage using black plates were identified and tested for O. tsutsugamushi by PCR. Results: Over an 18-month period, 131 small mammals of 14 species were collected in 5 districts. Seventy-eight of 131 small mammals were infested with chiggers, but all tissues were O. tsutsugamushi PCR negative. Eighteen species of chigger were identified and 1,609 were tested by PCR. A single pool of chiggers tested O. tsutsugamushi positive. Sera from 52 small mammals were tested by IFA, with 16 testing positive. Conclusions: These are the first molecular and serological data on O. tsutsugamushi in chiggers and small mammals in Laos. Further studies are needed to better understand the key vector species and ecology of scrub typhus in areas with high disease incidence in Laos.

Published
2022
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49. Underrecognized arthropod-borne and zoonotic pathogens in northern and northwestern Thailand: serological evidence and opportunities for awareness.

Author

Blacksell SD, Kantipong P, Watthanaworawit W, Turner C, Tanganuchitcharnchai A, Jintawon S, Laongnuanutit A, Nosten FH, Day NP, Paris DH, and Richards AL

Subjects
Adolescent, Adult, Aged, Anaplasma phagocytophilum isolation & purification, Animals, Antibodies, Bacterial blood, Arthropod Vectors microbiology, Bartonella henselae isolation & purification, Child, Child, Preschool, Cohort Studies, Coxiella burnetii isolation & purification, Ehrlichia chaffeensis isolation & purification, Humans, Immunoglobulin G blood, Infant, Middle Aged, Rickettsia isolation & purification, Seroepidemiologic Studies, Thailand epidemiology, Young Adult, Zoonoses, Anaplasma phagocytophilum immunology, Bartonella henselae immunology, Coxiella burnetii immunology, Ehrlichia chaffeensis immunology, Gram-Negative Bacterial Infections epidemiology, Rickettsia immunology
Abstract

Although scrub typhus and murine typhus are well-described tropical rickettsial illnesses, especially in Southeast Asia, only limited evidence is available for rickettsia-like pathogens contributing to the burden of undifferentiated febrile illness. Using commercially available kits, this study measured immunoglobulin G (IgG) antibody seroprevalence for Coxiella burnetii, Ehrlichia chaffeensis, Bartonella henselae, Anaplasma phagocytophilum, and spotted fever group rickettsiae (SFGR) in 375 patients enrolled in undifferentiated febrile illness studies at Chiangrai (northern Thailand) and Mae Sot (Thai-Myanmar border). Ehrlichia and SFGR were the most common causes of IgG seropositivity. A distinct relationship between age and seropositivity was found in Chiangrai with acquisition of IgG titers against Ehrlichia, Bartonella, Anaplasma, and SFGR in young adulthood, suggesting cumulative exposure to these pathogens. At Mae Sot, high early IgG titers against Ehrlichia and SFGR were common, whereas Anaplasma and Bartonella IgG titers increased at 50-60 years. Q fever associated with low IgG positivity at both study sites, with significantly higher prevalence at 30 years of age in Chiangrai. These data suggest that other rickettsial illnesses could contribute to the burden of febrile illness in Thailand and possibly adjacent regions. Improved diagnostics and better understanding of antibody longevity and cross-reactivity will improve identification and management of these easily treatable infectious diseases.

Published
2015
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