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Lu Liu,1,* Zili Tang,1,* Qian Zeng,1,* Wenchuan Qi,1 Ziyang Zhou,1 Daohong Chen,1 Dingjun Cai,1– 3 Ying Chen,1 Shiqi Sun,1 Siyao Gong,1 Bin He,1 Shuguang Yu,1– 3 Ling Zhao1– 3 1Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu City, Sichuan Province, People’s Republic of China; 2Acupuncture and Chronobiology Key Laboratory of Sichuan Province, Chengdu, Sichuan, People’s Republic of China; 3Key Laboratory of Acupuncture for Senile Disease (Chengdu University of Traditional Chinese Medicine), Ministry of Education, Chengdu City, Sichuan Province, China*These authors contributed equally to this workCorrespondence: Ling Zhao; Shuguang Yu, College of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, 1166 Liutai Avenue, Wenjiang District, Chengdu City, Sichuan Province, 611137, People’s Republic of China, Tel +86 28 6180 0000, Email zhaoling@cdutcm.edu.cn; ysg28588@126.comBackground: Electroacupuncture (EA), with varying stimulation intensities, has demonstrated therapeutic potentials in both animal and clinical studies for the treatment of chronic obstructive pulmonary disease (COPD). However, a comprehensive investigation of the intensity-related effects, particularly 1mA and 3mA of EA, and the underlying mechanisms remains lacking.Methods: A COPD rat model was established by prolonged exposure to cigarette smoke and intermittent intratracheal instillation of lipopolysaccharide. EA treatment was administered at acupoints BL13 (Feishu) and ST36 (Zusanli), 20 minutes daily for 2 weeks, with intensities of 1mA and 3mA. EA effectiveness was evaluated by pulmonary function, histopathological change, serum level of inflammatory cytokines, and level of oxidative stress markers in serum and lung tissues. Transcriptome profiling and weighted gene co-expression network analysis (WGCNA) were performed to reveal gene expression patterns and identify hub genes. Real-time quan

Here, we demonstrate continuous-flow separation of submicron particles under dielectrophoretic (DEP) force through a microfluidic device with 3D microelectrodes. Under optimal activation, the device is capable of effectively separating submicron particles at a high resolution of 200 nm. © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Continuous-flow electrokinetic enrichment/separation of nanoparticles is demonstrated in a device based on target nanoparticles railing along 3D microelectrode tracks. The device utilizes dielectrophoresis and electrohydrodynamic drag including electrothermal flow in high-conductive media and AC electroosmosis in low-conductive media. An enrichment efficiency of 100% is noted for 500 nm and 1 μm polystyrene spheres in media at a conductivity of 0.2 and 0.01 S/m. Size-based separation of the two is also shown efficient in deionized water using a modified design. © 2021 IEEE.

We demonstrate multifunctional silicon 3D viaduct microelectrodes for continuous-flow cell manipulation by railing and cell transfection under a modulated activation. The microelectrodes serve as tracks along which cells can rail under dielectrophoretic force and hydrodynamic drag. A modulated activation was introduced to achieve railing efficiency up to 80%. Furthermore, cell electroporation is realized through sinewave bursts applied with varying amplitude, duration and interval. Successful transfection is demonstrated by delivering 3 kDa dextran molecules into HEK293 cells with > 60% efficiency. The multifunctional 3D viaduct electrodes show great potential for continuous-flow rapid cell railing and sorting and efficient transfection.

This work presents a micro-fabricated planar bi-stable switch that requires no hook locking structure for switching between its two stable states. The hookless bi-stable mechanism is connected to a lever-amplifier driving by a single actuator and is built from four beams without sharp-angled design, improving durability and reducing fabrication difficulty. The setting and resetting voltages are 11 V and 12.5 V, respectively. By continuously performing more than 30,000 times of ON/OFF switching, the switch exhibits high robustness and offers potential for applications requiring a highly durable micro-switch.

A planar bi-stable switch with high durability based on latch-lock mechanism is presented. The bi-stable mechanism relies on a simple Y-shaped silicon cantilever to lock or release a latch pair. Setting and resetting of the switch are achieved by 12.5 V activation applied to a single electrothermal actuator. The mechanical switch exhibits high durability by remaining intact after ∼270,000 switching cycles, outlasting the driving actuator linkage that is used for testing its durability.

We report on electrokinetic manipulation of submicron particles in a device with microfabricated Si electrodes featuring segmented sidewalls acting as tracks guiding particles under hydrodynamic drag. Electrohydrodynamic drag, both AC electroosmosis in low-conductive medium and electrothermal flow in high-conductive medium, gives rise to fluid rolls that deliver particles to tracks where they are held under the influence of dielectrophoretic forces. Particles are being railed along tracks under pressure-driven flow to a downstream junction where tracks act as bridges, keeping particles on course within the main channel causing their post-junction enrichment. This outcome, however, is strongly coupled to electrode sidewall contours as demonstrated here. We also report on particle oscillations under strong AC electroosmosis. Specifically, particles in deionized water can be seen bouncing off tracks or circulating with convective rolls in and out of space beneath tracks at an oscillation frequency and amplitude depending on the activation frequency. These results collectively draw attention to the functional use of electrode sidewall contours for continuous-flow manipulation of particles, which are rarely explored in microfluidics and yet could lead to more effective designs for particle separation and enrichment. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.