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11 results on '"Tang, Xiang-Shan"'

5. Non-Starch Polysaccharides (NSP) Enzyme Improves the Nutrient Digestibility of Weaned Piglet Fed a Cottonseed Meal-Based Diets

Author

Lichuang Cai, Dingding Su, Xiaosong Wu, Jun Fang, Jiannan Ding, Zhichun Zhan, Tang Xiang‐Shan, Wang Jie, Ying Zhou, Wang Shengping, and Gang Liu

Subjects
chemistry.chemical_classification, Nutrient digestibility, Enzyme, General Veterinary, chemistry, Biochemistry, Non starch polysaccharides, Food science, Cottonseed meal, Agronomy and Crop Science
Published
2012
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6. Dietary supplementation with high-dose Bacillus subtilis or Lactobacillus reuteri modulates cellular and humoral immunities and improves performance in weaned piglets

Author

Wang, Sheng-Ping, Yang, Lingyuan, Tang, Xiang-Shan, Cai, Li-Chuang, Liu, Gang, Kong, Xiang-Feng, Blachier, Francois, Yin, Yu-Long, Res Ctr Healthy Breeding Livestock & Poultty, Hunan Engn & Res Ctr Anim Sci, Inst Subtrop Agr,Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Chinses Academy of Sciences, Coll Anim Sci & Technol, Hunan Agricultural University [Changsha], Res Ctr Healthy Breeding Livestock & Poultty, Hunan Engn & Res Ctr Anim Sci, Inst Subtrop Agr,Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Chinese Acad Sci, Inst Biol Resources, Jiangxi Academy of Agricultural Sciences, Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), AgroParisTech-Institut National de la Recherche Agronomique (INRA), National Basic Research Program of China [2009CB118800], Chinese Academy of Sciences [KZCX2-EW-412, KZCX2-YW-T07, KSCX2-EW-G-16, MXX281177], NSFC [30771558, 31001015, 31001016, 31072042, 30901040, 30901041, 30928018], Changsha City Key Project [K1003238-61], and Institut National de la Recherche Agronomique (INRA)-AgroParisTech

Subjects
AMINO-ACDIGESTIBILITY, LACTIC-ACID, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, growth performance, Lactobacillus reuteri, HULLESS BARLEY VARIETIES, ULTRA-FINE POWDER, immunity, GROWTH-PERFORMANCE, GROWING PIGS, BROILERS FED DIETS, high dose, piglet, APPARENT DIGESTIBILITY ILEAL, WEANLING PIGS, CASEIN-BASED DIETS, Bacillus subtilis
Abstract

Bacilli and Lactobacilli, especially high doses of them are often used as probiotics in piglets. The present study aimed to determine the effects of supplementing a piglet diet with high-dose Bacillus subtilis M-1 and Lactobacillus reuteri X-1. A total of 32 piglets with an initial body weight of 5.8 +/- 0.10 kg were weaned at 21 d of age and randomly allocated into one of four groups with eight replications of one pig: Group 1, assay diet supplemented with Bacillus subtilis M-1 (10(11) cfu kg(-1)); Group 2, assay diet supplemented with Lactobacillus reuteri X-1 (10(11) cfu kg(-1)); Group 3, assay diet supplemented with a combination of Bacillus subtilis M-1 and Lactobacillus reuteri X-1 (0.5x10(11) cfu kg(-1), respectively); control group, assay diet without bacterial supplement. Compared with the other groups, Group 2 showed an increase in feed intake (P > 0.05) and the highest ADG (P < 0.05), while Group I showed the best dry matter intake: gain ratio (P < 0.05). However, the three treatment groups (Group 1-3) all showed significant decreases in serum IgG and IgM concentrations (P < 0.05). Serum TNF-alpha, IL-6 and NO levels in Group 3 were decreased (P < 0.05) compared to those in the control group. Serum DAO and D-lactate concentrations were increased in all of the treatment groups (P < 0.05), compared to those in the control group. These findings show that the addition of high-dose Bacillus subtilis M-1 or Lactobacillus reuteri X-1 to the diet improved growth performance but impaired immunity in weaned piglets.

Published
2011

9. Dietary acidic calcium sulfate enhances growth, digestive enzyme activities, intestinal histology and resistance against Aeromonas hydrophilain juvenile largemouth bass, Micropterus salmoides

Author

Huang, He, Zhuang, Yi, Tang, Xiang-Shan, Wang, Nu-An, Yuan, Kun, and Zhong, Guo-Fang

Abstract

This study was conducted to investigate the effects of dietary supplementation with acidic calcium sulfate on growth, intestinal histology, and disease resistance of juvenile largemouth bass. Fish (19.88 ± 0.03 g) were fed on diets supplemented with 0 % (control), 1% acidic calcium sulfate (ACS1), 2 % acidic calcium sulfate (ACS2), 3 % acidic calcium sulfate (ACS3) and 0.03 % florfenicol (F) for 56 days. The results showed that the supplementation of 1 % acidic calcium sulfate to the diet significantly enhanced the growth performance (P < 0.05). Serum alanine aminotransferase activity in ACS2 and ACS3 groups was significantly higher than that in the control and ACS1 groups (P < 0.05). The serum alkaline phosphatase activity in ACS1 and F groups was significantly higher than that in the control and ACS3 groups (P < 0.05). The activity of serum superoxide dismutase in the control, ACS2 and ACS3 groups was significantly lower than that in ACS1 group (P < 0.05). Intestinal trypsin, amylase, and lipase activities increased significantly with increasing acidic calcium sulfate when compared to the control. (P < 0.05). The intestinal histology features showed that the villi height and width of ACS1 and ACS2 groups were significantly increased compared to that of the control (P < 0.05). The results of TOR pathway related genes in the liver showed that the expressions of TOR and ribosomal protein S6 of ACS1 group were significantly higher than the control, while the expression of 4E-BP1 was significantly decreased (P < 0.05). When challenged with Aeromonas hydrophila, the survival rate of the control was significantly lower than that of F, ACS1, and ACS2 groups (P < 0.05). In summary, the optimum amount of acidic calcium sulfate for this experiment is 1 %. 1 % acidic calcium sulfate can effectively improve the growth performance and disease resistance of juvenile largemouth bass.

Published
2023
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10. Quantitative Determination of Cucumber Mosaic Virus Genome RNAs in Virions by Real-Time Reverse Transcription-Polymerase Chain Reaction.

Author

FENG, Jun-Li, CHEN, Shao-Ning, TANG, Xiang-Shan, DING, Xian-Feng, DU, Zhi-You, and CHEN, Ji-Shuang

Subjects
CUCUMBER mosaic virus, POLYMERASE chain reaction, RNA viruses, QUANTITATIVE research, DILUTION, VIRAL genomes
Abstract

A real-time RT-PCR procedure using the green fluorescent dye SYBR Green I was developed for determining the absolute and relative copies of cucumber mosaic virus (CMV) genomic RNAs contained in purified virions. Primers specific to each CMV ORF were designed and selected. Sequences were then amplified with length varying from 61 to 153 bp. Using dilution series of CMV genome RNAs prepared by in vitro transcription as the standard samples, a good linear correlation was observed between their threshold cycle ( Ct) values and the logarithms of the initial template amounts. The copies of genomic RNA 1, RNA 2, RNA 3 and the subgenomic RNA 4 in CMV virions were quantified by this method, and the ratios were about 1.00:1.17:3.58:5.81. These results were confirmed by Lab-on-a-Chip and Northern blot hybridization assays. Our work is the first report concerning the relative amounts of different RNA fragments in CMV virions as a virus with tripartite genome. Edited by Ming-Hua XU [ABSTRACT FROM AUTHOR]

Published
2006
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11. Effects of folic acid on the performance of suckling piglets and sows during lactation.

Author

Wang SP, Yin YL, Qian Y, Li LL, Li FN, Tan BE, Tang XS, and Huang RL

Subjects
Ammonia blood, Animals, Animals, Suckling, Birth Weight, Blood Urea Nitrogen, Crosses, Genetic, Female, Folic Acid adverse effects, Folic Acid metabolism, Lactation blood, Lipid Metabolism, Litter Size, Male, Milk metabolism, Milk Proteins metabolism, Pregnancy, Sus scrofa physiology, Weaning, Weight Gain, Dietary Supplements, Folic Acid administration & dosage, Lactation metabolism, Maternal Nutritional Physiological Phenomena, Sus scrofa growth & development
Abstract

Background: The present research was conducted to investigate the influences of supplementation with different levels of folic acids in diet on the performance of lactating sows. Twenty Landrace × Yorkshire sows received the same basal corn-soybean diets (folic acid, 1.3 mg kg(-1)) from gestation to parturition (day 107). After parturition, sows were allotted to four treatments: control group (folic acid supplementation level, 0 mg kg(-1)), group 1 (12.5 mg kg(-1)), group 2 (50 mg kg(-1)) and group 3 (100 mg kg(-1)), with five replicates of one sow. The experiment lasted for 21 days., Results: (1) Folic acid increased milk production (P > 0.05). (2) Compared with the control, supplementation with folic acid (100 mg kg(-1)) increased the concentration of butter fat, total substance and non-lipoid substance significantly (P < 0.01), the concentration of milk protein was also significantly increased in group 2 and group 3 (P < 0.01) in milk. (3) Folic acid supplementation could increase litter weaning weight, average piglet weaning weight and average piglet daily gain (P > 0.05)., Conclusion: These results suggested that supplementation with folic acid in the diets of lactating sows increases milk production, improved milk quality and the performance of piglets., (Copyright © 2011 Society of Chemical Industry.)

Published
2011
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