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BackgroundTheADAMTS7locus was genome-wide significantly associated with coronary artery disease (CAD). Lack of the extracellular matrix (ECM) protease ADAMTS-7 was shown to reduce atherosclerotic plaque formation.ObjectiveTo identify molecular mechanisms and downstream targets of ADAMTS-7 mediating risk of atherosclerosis.MethodsTargets of ADAMTS-7 were identified by high-resolution mass spectrometry of atherosclerotic plaques from Apoe-/- and Apoe-/-Adamts7-/- mice. ECM proteins were identified using solubility profiling. Putative targets were validated using immunofluorescence,in vitrodegradation assays, co-immunoprecipitation, and Förster resonance energy transfer (FRET)-based protein-protein interaction assays.ADAMTS7expression was measured in fibrous caps of human carotid artery plaques.ResultsIn humans,ADAMTS7expression was higher in caps of unstable as compared to stable carotid plaques. Compared to Apoe-/- mice, atherosclerotic aortas of Apoe-/- mice lacking Adamts-7 (Apoe-/-Adamts7-/-) contained higher protein levels of tissue inhibitor of metalloproteases 1 (Timp-1). In co-immunoprecipitation experiments, the catalytic domain of ADAMTS-7 bound to TIMP-1, which was degraded in the presence of ADAMTS-7in vitro.ADAMTS-7 reduced the inhibitory capacity of TIMP-1 at its canonical target matrix metalloprotease 9 (MMP-9) As a downstream mechanism, we investigated collagen content in plaques of Apoe-/- and Apoe-/-Adamts7-/- mice after Western diet. Picrosirius red staining of the aortic root revealed less collagen as a readout of higher MMP-9 activity in Apoe-/- as compared to Apoe-/- Adamts7-/- mice. In order to facilitate high-throughput screening for ADAMTS-7 inhibitors with the aim to decrease TIMP-1 degradation, we designed a FRET-based assay targeting the ADAMTS-7 catalytic site.ConclusionADAMTS-7,which is induced in unstable atherosclerotic plaques, decreases TIMP-1 stability reducing its inhibitory effect on MMP-9, which is known to promote collagen degradation and is likewise genome-wide significantly associated with CAD. Disrupting the interaction of ADAMTS-7 and TIMP-1 might be a strategy to increase collagen content and plaque stability for reduction of atherosclerosis-related events.

Summary Background Vietnam has emerged as one of the world's leading success stories in responding to COVID-19. After a prolonged period of little to no transmission, there was an outbreak of unknown source in July, 2020, in the Da Nang region, but the outbreak was quickly suppressed. We aimed to use epidemiological, behavioural, demographic, and policy data from the COVID-19 outbreak in Da Nang to calibrate an agent-based model of COVID-19 transmission for Vietnam, and to estimate the risk of future outbreaks associated with reopening of international borders in the country. Methods For this modelling study, we used comprehensive data from June 15 to Oct 15, 2020, on testing, COVID-19 cases, and quarantine breaches within an agent-based model of SARS-CoV-2 transmission to model a COVID-19 outbreak in Da Nang in July, 2020. We applied this model to quantify the risk of future outbreaks in Vietnam in the 3 months after the reopening of international borders, under different behavioural scenarios, policy responses (ie, closure of workplaces and schools), and ongoing testing. Findings We estimated that the outbreak in Da Nang between July and August, 2020, resulted in substantial community transmission, and that higher levels of symptomatic testing could have mitigated this transmission. We estimated that the outbreak peaked on Aug 2, 2020, with an estimated 1060 active infections (95% projection interval 890–1280). If the population of Vietnam remains highly compliant with mask-wearing policies, our projections indicate that the epidemic would remain under control even if a small but steady flow of imported infections escaped quarantine into the community. However, if complacency increases and testing rates are relatively low (10% of symptomatic individuals are tested), the epidemic could rebound again, resulting in an estimated 2100 infections (95% projected interval 1050–3610) in 3 months. These outcomes could be mitigated if the behaviour of the general population responds dynamically to increases in locally acquired cases that exceed specific thresholds, but only if testing of symptomatic individuals is also increased. Interpretation The successful response to COVID-19 in Vietnam could be improved even further with higher levels of symptomatic testing. If the previous approaches are used in response to new COVID-19 outbreaks, epidemic control is possible even in the presence of low levels of imported cases. Funding Ministry of Science and Technology (Vietnam). Translation For the Vietnamese translation of the abstract see Supplementary Materials section.

PurposeThe purpose of this paper is twofold: first, to investigate strategies that external stakeholders can employ to affect construction project outcomes and, second, to identify essential requirements for utilising each strategy.Design/methodology/approachA new theoretical framework of stakeholder influence strategies was proposed and applied. The research design is a multi-case study, comprising four cases in the construction industry in Vietnam.FindingsSeven specific strategies were found, including inputs withholding, inputs compromising, communication, direct action, coalition building, conflict escalation and credibility building. When possessing project inputs, stakeholders can affect a project directly via a withholding or compromising strategy. Communication is available to those who have basic communicating skills; however, direct action is only employed by groups that include a large number of members. Objectors must have common interests or goals with their potential allies for using coalition building. Conflict escalation is restricted to communities having distinctive characteristics which can be used to create new problems sensibly, while credibility building is used by parties possessing adequate resources and expertise.Research limitations/implicationsThis study’s generalisability may be limited by the main source of data and the types of projects in the selected cases.Practical implicationsThis study provides directions for project managers to predict stakeholder influence by taking project inputs and utilisation requirements of the strategies into consideration.Originality/valueThis study is one of the first investigations on stakeholder-attributes-related requirements for utilising influence strategies in projects.

To assess the influence of nanopowder dispersion, performance was scrutinized between two plates. The upper one is permeable and below one is stretching and hot. The distance between two plates changes with time. The final equations were solved via MAPLE, and to analyze the impact of active factors, two significant functions were calculated. Two new formulas for Nu and En were suggested based on numerical data and outputs were summarized as contours and plots. Nu has various behaviors with change of α. Results depict that Nu declines about 21.29% with rise of α from 0 to 2. Positive impact of R on Nu is more significant for lower α and 37.44% augmentations were reported when R augments. Thinner boundary layer with rise of m results in higher Nu and 437% augmentation can be seen with rise of m from 0 to 3. With rise of particle interaction with rise of φ, temperature augments and greater heat transfer rate appears. Inclusion of nanopowders leads to augmentation in Nu about 3.54%. At first Nu rises with rise of R augments and then reduces, while opposite trend was observed for α. Nu for α = 2 is 1.13 times greater than that of α = 0 when R = 5, m = 1. To reach greater influence of nanoparticle dispersion, the lower values of m should be selected. As m augments, Nu reduces about 57.58% which means that a higher value of m leads to lower conduction mode.

Cyclic guanosine 3',5'-monophosphate (cGMP) is the key second messenger molecule in nitric oxide signaling. Its rapid generation and fate, but also its role in mediating acute cellular functions has been extensively studied. In the past years, genetic studies suggested an important role for cGMP in affecting the risk of chronic cardiovascular diseases, for example, coronary artery disease and myocardial infarction. Here, we review the role of cGMP in atherosclerosis and other cardiovascular diseases and discuss recent genetic findings and identified mechanisms. Finally, we highlight open questions and promising research topics.

AimThe role of platelets in atherosclerosis remains incompletely understood. Variants in genes encoding the soluble guanylyl cyclase (sGC) in platelets are associated with coronary artery disease (CAD) risk. Here we sought to investigate the contribution of platelet sGC to atherosclerosis and the therapeutic potential of targeting sGC in atherosclerosis.Methods and ResultsWe genetically deleted sGC in platelets of atherosclerosis-prone Ldlr-/- mice. By intravital fluorescence microscopy such Pf4-Cre+Gucy1b1flox/floxLdlr-/- mice displayed enhanced leukocyte adhesion to atherosclerotic plaques in comparison with their litter mates. Moreover, histological and flow cytometry analyses revealed more numerous inflammatory leukocytes and larger plaque sizes in aortic tissue of Ldlr-/- mice lacking sGC in platelets. In vitro, supernatant from activated platelets lacking sGC promoted leukocyte adhesion to endothelial cells (EC) via enhanced EC activation. Using cytokine profiling, we identified reduced angiopoietin-1 release by Pf4-Cre+Gucy1b1flox/flox and human GUCY1A1 risk allele carrier platelets to be responsible for enhanced activation of EC and subsequent leukocyte adhesion. Pharmacological sGC stimulation increased platelet angiopoietin-1 release in vitro and reduced recruitment of adoptively transferred leukocytes in Ldlr-/- mice fed a Western diet. Pharmacological sGC stimulation further reduced atherosclerotic plaque formation and vascular inflammation.ConclusionLoss of sGC in platelets contributes to atherosclerotic plaque formation via reduced release of the soluble factor angiopoietin-1 and, subsequently, enhanced leukocyte recruitment. Pharmacological sGC stimulation might represent a novel therapeutic strategy to prevent and treat CAD.Translational perspectiveReduced platelet soluble guanylyl cyclase activity contributes to atherosclerotic plaque formation and vascular inflammation. Stimulators of the soluble guanylyl cyclase, an emerging class of drugs already used in pulmonary hypertension and heart failure, are able to reduce atherosclerosis and inflammation in this preclinical model. Together with evidence from human genetics, our findings suggest a promising role of soluble guanylyl cyclase stimulation to prevent coronary artery disease.

Introduction Hospitals are a key source of information for the early identification of emerging disease outbreaks and acute public health events for risk assessment, decision-making and public health response. The objective of this study was to identify potential facilitators and barriers for event reporting from the curative sector to the preventive medicine sector in Viet Nam. Methods In 2016, we conducted 18 semi-structured, in-depth interviews, as well as nine focus group discussions, with representatives from the curative and preventive medicine sectors in four provinces. We transcribed the interviews and focus group discussions and used thematic analysis to identify the factors that appeared to affect public health event reporting. Results We identified five major themes. First, the lack of a legal framework to guide reporting meant hospital staff relied on internal procedures that varied from hospital to hospital, which sometimes delayed reporting. Second, participants stated the importance of an enabling environment, such as leadership support and having focal points for reporting, to facilitate reporting. Third, participants described the potential benefits of reporting, such as support provided during outbreaks and information received about local outbreaks. Fourth, some challenges prohibited timely reporting such as not perceiving reporting to be the task of the curative sector and hesitancy to report without laboratory confirmation. Finally, limited resources and specialist capacities in remote areas hindered timely detection and reporting of unusual events. Discussion This study identified potential opportunities to promote the detection and reporting of unusual events from health-care workers to the public health sector, and thus to improve the overall health security system in Viet Nam.The influenza virus is a respiratory pathogen that is transmitted through respiratory droplets. 1 During seasonal influenza epidemics, high attack rates cause a significant public health burden. 2 The infection is usually self-limited in young adults but can lead to severe infections in people in high-risk groups, including elderly people (> 65 years old), pregnant women, children aged 6-59 months and adults with chronic illnesses. 3.

The use of cryptography algorithms based on hardware to protect the security and privacy of the loT systems is in huge demand. However, hardware-based security systems are similar to software-based in that they can be attacked by a type of malicious code called malware. In this article, we used the self-applied cryptography algorithm on the hardware-based to protect the data security of loT systems, and that gives the trustee than using software-based of hardware-based inform of ASIC chip. Detailly, we have developed an SoC FPGA system including main factors are a built-in ARM processor core, a self-implemented IP core acting as 128-bit AES co-processors, and SDRAM memory. Furthermore, this system has been placed on the DE10-Standard board and ran with an application in Linux operations. Besides, we have applied the DMA technique to obtain high-speed data transfer. As a result, our cryptosystem is compact and can achieve high performance with low frequencies. Details, the DMA Controller and 128-bit AES IP core operate at a frequency of 150 MHz, and they can reach the speed of 1200 Mbps and above 700 Mbps, respectively.

Although understanding the influence of external stakeholders is crucial to achieving project success, little attention has been given to investigate their specific influence strategies. Based on a modified theoretical framework, we conducted a multiple-case study to explore common combinations and sequencings of specific actions that these stakeholders take to affect construction projects. Seven specific influence strategies were found: (i) inputs withholding, (ii) inputs compromising, (iii) communication, (iv) direct action, (v) coalition building, (vi) conflict escalation and (vii) credibility building. Stakeholders combined the last five strategies in three pairs persuading decision makers to take action. Coalition building - communication was the most prevalent combination that stakeholders use to influence decision-makers. The combination of communication and credibility building was found in cases having environmental disputes. Direct action and conflict escalation were exerted together in projects affecting the locals adversely. Some common patterns of stakeholder strategic actions were also observed. First, direct action came into existence after communication, followed by conflict escalation. Second, after establishing their credibility via persuasive evidence, experts’ judgements and the like, stakeholders used those outcomes to communicate to decision-makers. We emphasize the need for full and honest engagement with stakeholders to prevent their adverse influence on construction projects. Refereed/Peer-reviewed

Despite the advancements in digital technologies, the current building design examination practice is 2D and paper-based, and a large number of 2D plans and drawings need to be collated and interpreted to examine if the proposed designs comply with building regulations. Subsequently, it is prone to human errors that make sustainable and consistent design difficult. Although Building Information Modelling (BIM) is recognised as a means to transform the current practice into a more sustainable and productive practice, BIM has rarely been adopted in building design examination. This research aims to identify the reasons for the low uptake of BIM and to examine the feasibility of BIM for building design examination through a focus group interview and workshop. A lack of proper BIM training is identified as the most critical barrier to adopting BIM. Building design examiners indicate that BIM adoption requires consistent efforts with empirical errors, since the existing work processes are not flexible enough to embrace BIM instantly without proper BIM training. An average of three days can be saved by using BIM for a building regulations check. This research revealed that BIM is feasible for building regulation checking, and the low uptake is mainly caused by a lack of awareness of the BIM capabilities. Refereed/Peer-reviewed

Understanding external stakeholders’ influence is essential to achieving success; however, little attention has been paid to investigating their indirect influence on projects. This study aims to explore indirect pathways that external stakeholders normally pursue to affect construction projects and to develop a framework of stakeholder-influencing pathways. The authors adopted abductive reasoning, using a preliminary framework. Based on the framework, four process-tracing case studies in the Vietnamese construction industry were conducted for data collection and analysis. Newspapers were the main source of data, complemented by interviews and other archive data, such as press releases, petitions, blog postings, and official documents. All cases confirmed the pathway through which stakeholders induce decision-makers to exert pressure related to a project. Also, some unanticipated results were found: first, two additional pathways, comprising two or three causally ordered mediators, can be employed to affect projects; second, stakeholders tend to use multiple influence pathways in which some actors, such as governmental authorities and the public, have a high rate of occurrence; and third, a mediator can be affected by more than one successor, whereas some mediators are capable of influencing various predecessors. Accordingly, the preliminary framework was revised to match the unanticipated results. This study explores a preliminary mechanism that transmits stakeholder influences to a project via multiple causally ordered actors. It is also the first comprehensive investigation into stakeholder influence pathways in the built environment. The findings can assist managers in stakeholder prioritization and anticipating their influences on projects. Refereed/Peer-reviewed

BackgroundVietnam has emerged as one of the world’s leading success stories in responding to COVID-19. After prolonged zero-low transmission, a summer outbreak of unknown source at Da Nang caused the country’s first COVID-19 deaths, but was quickly suppressed. Vietnam recently reopened its borders to international travelers. Understanding the attendant risks and how to minimize them is crucial as Vietnam moves into this new phase.MethodsWe create an agent-based model of COVID-19 in Vietnam, using regional testing data and a detailed linelist of the 1,014 COVID-19 cases, including 35 deaths, identified across Vietnam. We investigate the Da Nang outbreak, and quantify the risk of another outbreak under different assumptions about behavioral/policy responses and ongoing testing.ResultsThe Da Nang outbreak, although rapidly contained once detected, nevertheless caused significant community transmission before it was detected; higher symptomatic testing could have mitigated this. If testing levels do not increase, the adoption of past policies in response to newly-detected cases may reduce the size of potential outbreaks but will not prevent them. Compared to a baseline symptomatic testing rate of 10%, we estimate half as many infections under a 20% testing rate, and a quarter as many with 40-50% testing rates, over the four months following border reopenings.ConclusionsVietnam’s success in controlling COVID-19 is largely attributable to its rapid response to detected outbreaks, but the speed of response could be improved even further with higher levels of symptomatic testing.

An assembly line is made from different machines and a machine can contain different stations and function carriers. Each function carrier even is performed by different standard function carrier variants. The designer can calculate and select the available function carrier variants to construct into a new machine. The advantages of using the available function carriers are the low cost, reduction of design and manufacture time and improvement of machine working life. If a machine has n function carriers, each function carrier contains m variants. Hence, there are nm combinations to make the machine. The number of these combinations increase rapidly according to a large number of function carriers. To select the suitable function carrier variants subject to optimal cycle time and total purchasing cost, the designer cannot manually select the best solution from so many options. Nowadays, there is no effective method to solve this problem. To solve this problem, this paper will set up the operating cycles for an assembly machine and establish linear optimization in standard form for choosing the best function carrier variants from the given database. To minimize both the cycle time and total purchasing cost of an assembly machine, the linear programming must contain two stages and run in sequence. The large linear optimization is programed and solved by using the IBM ILOG CPLEX Optimizer. The optimal results will be exported to tables in a database. This makes the designer easy evaluate and select the best solution. In addition, designer can expand the scope of study to design other machines.

Background Simulation exercises can functionally validate World Health Organization (WHO) International Health Regulations (IHR 2005) core capacities. In 2018, the Vietnam Ministry of Health (MOH) conducted a full-scale exercise (FSX) in response to cases of severe viral pneumonia with subsequent laboratory confirmation for Middle East Respiratory Syndrome Coronavirus (MERS-CoV) to evaluate the country’s early warning and response capabilities for high-risk events. Methods An exercise planning team designed a complex fictitious scenario beginning with one case of severe viral pneumonia presenting at the hospital level and developed all the materials required for the exercise. Actors, controllers and evaluators were trained. In August 2018, a 3-day exercise was conducted in Quang Ninh province and Hanoi city, with participation of public health partners at the community, district, province, regional and national levels. Immediate debriefings and an after-action review were conducted after all exercise activities. Participants assessed overall exercise design, conduction and usefulness. Results FSX findings demonstrated that the event-based surveillance component of the MOH surveillance system worked optimally at different administrative levels. Detection and reporting of signals at the community and health facility levels were appropriate. Triage, verification and risk assessment were successfully implemented to identify a high-risk event and trigger timely response. The FSX identified infection control, coordination with internal and external response partners and process documentation as response challenges. Participants positively evaluated the exercise training and design. Conclusions This exercise documents the value of exercising surveillance capabilities as part of a real-time operational scenario before facing a true emergency. The timing of this exercise and choice of disease scenario was particularly fortuitous given the subsequent appearance of COVID-19. As a result of this exercise and subsequent improvements made by the MOH, the country may have been better able to deal with the emergence of SARS-CoV-2 and contain it.

International audience; Cultured marble is a blend of unsaturated polyester and calcium carbonate that is combined with pigments and additives to produce a wide range of colours and realistic, natural-looking patterns. This study aims to investigate process conditions (catalyst, temperature, cured time, etc …) of cultured marble at high temperatures. The optimized process conditions are obtained by thermal analysis from DSC with a temperature of 110°C and curing time of 20 min, respectively. The optimum catalyst for process conditions in a bulk moulding compound (BMC) machine is 2 phr of benzoyl peroxide (BPO). The styrene amount in UP resin is determined at 37.40% by the Gas chromatography-mass spectroscopy – headspace (GCMS-HS) technique. Anhydride maleic (MA) is found to improve outstanding mechanical properties in terms of flexural properties, the strength of impact, and the resistance of volume shrinkage of UP resin in cultured marble material.

Security is an important issue in the era of IoT, where a large number of devices are connected. In this paper, we propose a design to enhance the security of the IoT connection using SoC FPGA platforms. The design is a successful combination of the powerful processing and system management capabilities of ARM processors and the flexibility and customizability of FPGA technology. Particularly, we have developed a Digital Signature cryptosystem on a DE10-Standard SoC FPGA board using the built-in ARM processor core and two self-developed IP cores acting as 1024-bit RSA and 256-bit SHA co-processors. Furthermore, we also applied the DMA technique to achieve high-speed data transfer. As a result, the proposed cryptosystem is compact but achieves high performance even with low frequencies. In more detail, the DMA operating at the frequency of 150 MHz can achieve a speed of 1200 Mbps. Also, the 1024-bit RSA core and the 256-bit SHA operating at the frequencies of 50 MHz and 100 MHz have throughputs of 25 Kbps and above 700 Mbps, respectively.

Surveillance and outbreak reporting systems in Vietnam required improvements to function effectively as early warning and response systems. Accordingly, the Ministry of Health of Vietnam, in collaboration with the US Centers for Disease Control and Prevention, launched a pilot project in 2016 focusing on community and hospital event–based surveillance. The pilot was implemented in 4 of Vietnam’s 63 provinces. The pilot demonstrated that event-based surveillance resulted in early detection and reporting of outbreaks, improved collaboration between the healthcare facilities and preventive sectors of the ministry, and increased community participation in surveillance and reporting.

Background In 2016, as a component of the Global Health Security Agenda, the Vietnam Ministry of Health expanded its existing influenza sentinel surveillance for severe acute respiratory infections (SARI) to include testing for 7 additional viral respiratory pathogens. This article describes the steps taken to implement expanded SARI surveillance in Vietnam and reports data from 1 year of expanded surveillance. Methods The process of expanding the suite of pathogens for routine testing by real‐time reverse transcriptase–polymerase chain reaction (rRT‐PCR) included laboratory trainings, procurement/distribution of reagents, and strengthening and aligning SARI surveillance epidemiology practices at sentinel sites and regional institutes (RI). Results Surveillance data showed that of 4003 specimens tested by the RI laboratories, 20.2% (n = 810) were positive for influenza virus. Of the 3193 influenza‐negative specimens, 41.8% (n = 1337) were positive for at least 1 non‐influenza respiratory virus, of which 16.2% (n = 518), 13.4% (n = 428), and 9.6% (n = 308) tested positive for respiratory syncytial virus, rhinovirus, and adenovirus, respectively. Conclusions The Government of Vietnam has demonstrated that expanding respiratory viral surveillance by strengthening and building upon an influenza platform is feasible, efficient, and practical.