Search

Vibriospp. have been widely studied for their unique properties such as pathogenicity and quorum sensing (QS) abilities. This article presents the identification ofVibrio variabilisstrain T01 isolated from Malaysian coastal waters. Strain T01 ofV. variabiliswas identified to produce QS molecules as tested using a biosensor. High-resolution tandem mass spectrometry was used to identifyN-acyl homoserine lactone (AHL) profiles of strain T01. Three AHLs,viz. N-hexanoyl-L-homoserine lactone (C6-HSL),N-(3-oxodecanoyl)-L-homoserine lactone (3-oxo-C10-HSL) andN-dodecanoyl-L-homoserine lactone (C12-HSL), were confirmed. To the authors' knowledge, this is the first documentation of AHL profiles fromV. variabilisstrain T01, which expands the number of QS members inVibriospp. [ABSTRACT FROM AUTHOR]

N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS properties, environmental water sampling was conducted. Here we report the preliminary screening of AHL production using Chromobacterium violaceum CV026 and Escherichia coli [pSB401] as AHL biosensors. The 16S rDNA gene sequence of isolate M009 showed the highest sequence similarity to Pantoea stewartii S9-116, which is a plant pathogen. The isolated Pantoea sp. was confirmed to produce N-3-oxohexanoyl-L-HSL (3-oxo-C6-HSL) through analysis of high resolution mass tandem mass spectrometry. [ABSTRACT FROM AUTHOR]

N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS properties, environmental water sampling was conducted. Here we report the preliminary screening of AHL production using Chromobacterium violaceum CV026 and Escherichia coli [pSB401] as AHL biosensors. The 16S rDNA gene sequence of isolate M009 showed the highest sequence similarity to Pantoea stewartii S9-116, which is a plant pathogen. The isolated Pantoea sp. was confirmed to produce N-3-oxohexanoyl-L-HSL (3-oxo-C6-HSL) through analysis of high resolution mass tandem mass spectrometry.