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162 results on '"Tambong, James"'

7. Rhizobium acaciae sp. nov., a new nitrogen-fixing symbiovar isolated from root nodules of Acacia saligna in Tunisia

Author

Hsouna, Jihed, primary, Ilahi, Houda, additional, Han, Jia-Cheng, additional, Gritli, Takwa, additional, Ellouze, Walid, additional, Zhang, Xiao Xia, additional, Mansouri, Maroua, additional, Rahi, Praveen, additional, El Idrissi, Mustapha Missbah, additional, Lamrabet, Mouad, additional, Oubla, M'hamed, additional, Courty, Pierre Emmanuel, additional, Wipf, Daniel, additional, Tambong, James T., additional, and Mnasri, Bacem, additional

Published
2023
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8. Phylogenomic Insights on the Xanthomonas translucens Complex, and Development of a TaqMan Real-Time Assay for Specific Detection of pv. translucens on Barley.

Author

Tambong, James T., Xu, Renlin, Fleitas, Maria Constanza, Lipu Wang, Hubbard, Keith, and Kutcher, Randy

Subjects
*NUCLEIC acid hybridization, *XANTHOMONAS, *PROTEOMICS, *COMPARATIVE genomics, *HOST plants, *BACTERIAL wilt diseases, *HORDEUM, *BARLEY
Abstract

The reemergence and spread of Xanthomonas translucens, the causal agent of bacterial leaf streak in cereal crops and wilt in turfgrass and forage species, is a concern to growers in the United States and Canada. The pathogen is seedborne and listed as an A2 quarantine organism by EPPO, making it a major constraint to international trade and exchange of germplasm. The pathovar concept of the X. translucens group is confusing due to overlapping of plant host ranges and specificity. Here, comparative genomics, phylogenomics, and 81 up-to-date bacterial core gene set (ubcg2) were used to assign the pathovars of X. translucens into three genetically and taxonomically distinct clusters. The study also showed that whole genome-based digital DNA-DNA hybridization unambiguously can differentiate the pvs. translucens and undulosa. Orthologous gene and proteome matrix analyses suggest that the cluster consisting of graminis, poae, arrhenatheri, phlei, and phleipratensis is very divergent. Whole-genome data were exploited to develop the first pathovar-specific TaqMan real-time PCR tool for detection of pv. translucens on barley. Specificity of the TaqMan assay was validated using 62 Xanthomonas and non-Xanthomonas strains as well as growth chamber-inoculated and naturally infected barley leaves. Sensitivity levels of 0.1 pg (purified DNA) and 23 CFUs per reaction (direct culture) compared favorably with other previously reported real-time PCR assays. The phylogenomics data reported here suggest that the clusters could constitute novel taxonomic units or new species. Finally, the pathovar-specific diagnostic tool will have significant benefits to growers and facilitate international exchange of barley germplasm and trade. [ABSTRACT FROM AUTHOR]

Published
2023
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12. Genotypic and symbiotic diversity studies of rhizobia nodulating Acacia saligna in Tunisia reveal two novel symbiovars within the Rhizobium leguminosarum complex and Bradyrhizobium

Author

Hsouna, Jihed, primary, Gritli, Takwa, additional, Ilahi, Houda, additional, Ellouze, Walid, additional, Mansouri, Maroua, additional, Chihaoui, Saif-allah, additional, Bouhnik, Omar, additional, Missbah El Idrissi, Mustapha, additional, Abdelmoumen, Hanaa, additional, Wipf, Daniel, additional, Courty, Pierre Emmanuel, additional, Bekki, Abdelkader, additional, Tambong, James T., additional, and Mnasri, Bacem, additional

Published
2022
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18. Molecular Analysis of Bacterial Isolates From Necrotic Wheat Leaf Lesions Caused by Xanthomonas translucens, and Description of Three Putative Novel Species, Sphingomonas albertensis sp. nov., Pseudomonas triticumensis sp. nov. and Pseudomonas foliumensis sp. nov.

Author

Tambong, James T., primary, Xu, Renlin, additional, Gerdis, Suzanne, additional, Daniels, Greg C., additional, Chabot, Denise, additional, Hubbard, Keith, additional, and Harding, Michael W., additional

Published
2021
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27. Bacterial Pathogens of Wheat : Symptoms, Distribution, Identification, and Taxonomy

Author

Tambong, James T.

Subjects
Technology & Engineering / Agriculture
Abstract

Bacterial pathogens are significant biotic factors of wheat, a globally important source of carbohydrates. The diseases caused by these pathogens are reported to reduce annual wheat production by about 10% and up to 40% in severe infections occurring early in the growth period. This chapter presents current information on the symptoms, distribution, identification, and taxonomy of key bacterial pathogens of wheat with a focus on the seed-borne bacterium, Xanthomonas translucens pv. undulosa, the causative agent of the leaf streak and black chaff disease. Other wheat-pathogenic bacterial pathogens addressed in the chapter are Pseudomonas syringae pv. syringae, the causal agent of bacterial leaf blight; P. syringae pv. atrofaciens that cause the basal glume rot; Pseudomonas fuscovaginae, the causal agent of the bacterial brown sheath; Erwinia rhapontici, the causal agent of the pink seed of wheat; Pseudomonas cichorii, the causative agent of wheat stem melanosis; Clavibacter tessellarius is responsible for the bacterial mosaic of wheat as well as other minor bacterial pathogens. Finally, the chapter proposed the use of genome-based tools for the accurate identification and classification of bacterial pathogens of wheat.

Published
2019

32. Comparative genomics of Clavibacter michiganensis subspecies, pathogens of important agricultural crops

Author

Tambong, James T.

Subjects
0301 basic medicine, Proteome, Proteomes, lcsh:Medicine, Plant Science, Subspecies, Plant Genetics, Biochemistry, Genome, Carbon utilization, Database and Informatics Methods, RNA, Ribosomal, 16S, Databases, Genetic, Plant Genomics, lcsh:Science, Phylogeny, Genetics, Multidisciplinary, Chromosome Biology, Genomics, Genome project, Genomic Databases, Clavibacter michiganensis, Research Article, Biotechnology, DNA, Bacterial, Virulence Factors, Pseudogene, 030106 microbiology, Biology, Research and Analysis Methods, Chromosomes, 03 medical and health sciences, Species Specificity, DNA-binding proteins, Actinomycetales, Comparative genomics, lcsh:R, Biology and Life Sciences, Computational Biology, Proteins, Cell Biology, Comparative Genomics, Genome Analysis, biology.organism_classification, Chaperone Proteins, Biological Databases, 030104 developmental biology, Plant Biotechnology, lcsh:Q, Mobile genetic elements
Abstract

Subspecies of Clavibacter michiganensis are important phytobacterial pathogens causing devastating diseases in several agricultural crops. The genome organizations of these pathogens are poorly understood. Here, the complete genomes of 5 subspecies (C. michiganensis subsp. michiganensis, Cmi; C. michiganensis subsp. sepedonicus, Cms; C. michiganensis subsp. nebraskensis, Cmn; C. michiganensis subsp. insidiosus, Cmi and C. michiganensis subsp. capsici, Cmc) were analyzed. This study assessed the taxonomic position of the subspecies based on 16S rRNA and genome-based DNA homology and concludes that there is ample evidence to elevate some of the subspecies to species-level. Comparative genomics analysis indicated distinct genomic features evident on the DNA structural atlases and annotation features. Based on orthologous gene analysis, about 2300 CDSs are shared across all the subspecies; and Cms showed the highest number of subspecies-specific CDS, most of which are mobile elements suggesting that Cms could be more prone to translocation of foreign genes. Cms and Cmi had the highest number of pseudogenes, an indication of potential degenerating genomes. The stress response factors that may be involved in cold/heat shock, detoxification, oxidative stress, osmoregulation, and carbon utilization are outlined. For example, the wco-cluster encoding for extracellular polysaccharide II is highly conserved while the sucrose-6-phosphate hydrolase that catalyzes the hydrolysis of sucrose-6-phosphate yielding glucose-6-phosphate and fructose is highly divergent. A unique second form of the enzyme is only present in Cmn NCPPB 2581. Also, twenty-eight plasmid-borne CDSs in the other subspecies were found to have homologues in the chromosomal genome of Cmn which is known not to carry plasmids. These CDSs include pathogenesis-related factors such as Endocellulases E1 and Beta-glucosidase. The results presented here provide an insight of the functional organization of the genomes of five core C. michiganensis subspecies, enabling a better understanding of these phytobacteria.

Published
2017

38. Genome Analysis and Development of a Multiplex TaqMan Real-Time PCR for Specific Identification and Detection of Clavibacter michiganensis subsp. nebraskensis

Author

Tambong, James T., primary, Xu, Renlin, additional, Daayf, Fouad, additional, Brière, Stephan, additional, Bilodeau, Guillaume J., additional, Tropiano, Raymond, additional, Hartke, Allison, additional, Reid, Lana M., additional, Cott, Morgan, additional, Cote, Tammy, additional, and Agarkova, Irina, additional

Published
2016
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40. Identification, characterization and description of Arcobacter faecis sp. nov., isolated from a human waste septic tank

Author

Whiteduck-Léveillée, Kerri, primary, Whiteduck-Léveillée, Jenni, additional, Cloutier, Michel, additional, Tambong, James T., additional, Xu, Renlin, additional, Topp, Edward, additional, Arts, Michael T., additional, Chao, Jerry, additional, Adam, Zaky, additional, Lévesque, C. André, additional, Lapen, David R., additional, Villemur, Richard, additional, and Khan, Izhar U.H., additional

Published
2016
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41. Evidence of the Interaction Between Crop Species and Organic Amendments: Modelling of the Differential Grain Yield Response of Wheat, Soybean, and Canola to Organic Amendments

Author

Nkoa, Roger, Ondoua, Boris, Voroney, Paul, and Tambong, James

Subjects
fungi, food and beverages, complex mixtures, Crop Production/Industries
Abstract

Knowledge on the interaction between plants and organic amendments is critical for the basic understanding of agroecosystems sustainability. Organic amendments are of great interest in agriculture by virtue of their ability to restore lost soil organic carbon in eroded or conventionally cultivated soils. The major objective of this study was to demonstrate and model the differential response of crop species to organic amendments. Despite the potential of such an interaction to improve crop production, it has never been formally demonstrated in a planned experiment. A two-year greenhouse experiment set as 3×3×5 factorial in a strip-split plot design was conducted. The effects of crop species, type of organic amendment, and application rates on grain yield of soybean, canola, and wheat were evaluated. To account for the asymmetry of the concave responses of soybean, mathematical transcendental models were fitted, for the first time, to yield data. The interaction between crop species and amended soils was highly significant. Soybean displayed concave transcendental yield responses whereas canola and wheat exhibited negative exponential responses, irrespective of the type of amendment. Turkey compost outperformed turkey litter and beef manure by 30% and 52%, respectively, with respect to soybean production; whereas turkey litter outperformed turkey compost and beef manure by 144% and 264%, respectively, with respect to canola and wheat production. It is concluded that in greenhouse settings and perhaps field conditions, growth and development of crop species can be enhanced by matching the specific characteristics of organic amendments to the specific nutrients demand of crop species.

Published
2014
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47. Arcobacter lanthieri sp. nov., isolated from pig and dairy cattle manure

Author

Whiteduck-Léveillée, Kerri, primary, Whiteduck-Léveillée, Jenni, additional, Cloutier, Michel, additional, Tambong, James T., additional, Xu, Renlin, additional, Topp, Edward, additional, Arts, Michael T., additional, Chao, Jerry, additional, Adam, Zaky, additional, André Lévesque, C., additional, Lapen, David R., additional, Villemur, Richard, additional, Talbot, Guylaine, additional, and Khan, Izhar U. H., additional

Published
2015
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49. Rhizosphere persistence of three Pythium oligandrum strains in tomato soilless culture assessed by DNA macroarrayand real-time PCR

Author

Le Floch, Gaétan, Tambong, James, Vallance, Jessica, Tirilly, Yves, Lévesque, André, Rey, Patrice, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Université de Brest (UBO), Central Experimental Farm, and Agriculture and Agri-Food [Ottawa] (AAFC)

Subjects
quantitative PCR, [SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy, food and beverages, biocontrol, Pythium dissotocum, oomycetes, DNA array, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract

The definitive version is available at ww3.interscience.wiley.com; International audience; In tomato soilless culture, plant-disease optimal control and growth promotion are achieved when the rhizosphere is heavily colonized by the biocontrol agent Pythium oligandrum. Discrepancies in performance are generally attributed to the poor persistence of P. oligandrum on roots. In this study, three selected strains of P. oligandrum were introduced into the rhizosphere of greenhouse-grown tomato plants, and their persistence was assessed by DNA macroarray hybridization and real-time PCR. The experimental data from DNA detection and plate counting were compared. PCR-based methods detected P. oligandrum throughout the 6-month growing season, whereas plate counting indicated its presence only over the first 3 months. Moreover, the DNA array method provided information about the various Pythium species present in the rhizosphere: P. dissotocum was frequently detected on roots of plants, without distinction between plants inoculated or not inoculated with the antagonist. The detection of other Pythium species was noticed sporadically (P. ultimum, P. sylvaticum and P. intermedium), independent of the treatment. Even though the yield enhancement is not significant throughout the entire growing season, data obtained from epidemiological studies demonstrate an enhancement of P. oligandrum persistence on the rhizosphere of plants and less use of mycoparasitism.

Published
2007
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