26 results on '"Tamang M"'
Search Results
2. Variation in Tree Density, Biomass and Carbon Stock across an Altitudinal Gradient under Large Cardamom Agroforestry System of Darjeeling Himalaya
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., Vineeta, primary, Sarkar, B. C., additional, Tamang, M., additional, Shukla, Gopal, additional, N, Shahina N, additional, Nath, Arun Jyoti, additional, and Chakravarty, Sumit, additional
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- 2023
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3. Anemia among school children in eastern Nepal
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Khatiwada, S., primary, Gelal, B., additional, Gautam, S., additional, Tamang, M. K., additional, Shakya, P. R., additional, Lamsal, M., additional, and Baral, N., additional
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- 2015
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4. OKkIO alla SALUTE: promozione della salute e crescita sana nei bambini della scuola primaria [Promotion of healthy life style and growth in primary school children (OKkio alla SALUTE)]
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Spinelli, A., Baglio, G., Cattaneo, C., Fontana, G., Lamberti, A., Gruppo OKkio alla SALUTE, Coorte PROFEA anno 2006, Baglio, G. I., Binkin, N., Bucciarelli, M., Perra, A., Galeone, D., Silani, M. T., Mastantuono, Edvige, Teti, Silvana, Censi, Laura, D'Addesa, Dina, D'Amicis, A., Ciglia, Antonio, Di Giacomo, M., Sorrentino, G., Fersini, G., Perri, G., De Lorenzo, G., Angelini, P., Di Martino, E., Carletti, C., Rincorosi, R., Cairella, G., Castronuovo, E., Pascali, F., Oreste, P., Giostra, G., Tagliavento, G., Selvaggi, T. I., Caputo, M., Anelli, S., Pomo, V., Arras, P., Cattina, G., Cernigliaro, A., Rizzo, S., Giacchi, M., Lazzeri, G., Cristofori, M., Giaimo, M, Covarino, Anna Maria, D'Alessandro, G., Riccardo, G., Tamang, M., Piffer, S., Baldi, A., Balducci, M., Bilei, S., Callipari, O., De Luca, A., Marrone, A., Mazzarella, G., and Silvestri, A.
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- 2008
5. Iodized Salt Use and Salt Iodine Content among Household Salts from Six Districts of Eastern Nepal.
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Khatiwada, S., Gelal, B., Tamang, M. K., K. C. R., Singh, S., Lamsal, M., and Baral, N.
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- 2014
6. Control of Lantana in non-cropped area and in tea plantation
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Singh, R. D., primary, Singh, Brajinder, additional, Sud, R. K., additional, Tamang, M. B., additional, and Chakrabarty, D. N., additional
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- 1997
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7. Iodized Salt Use and Salt Iodine Content among Household Salts from Six Districts of Eastern Nepal
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Khatiwada, S., Gelal, B., Tamang, M. K., Rajendra KC, Singh, S., Lamsal, M., and Baral, N.
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Family Characteristics ,Cross-Sectional Studies ,Nepal ,Humans ,Sodium Chloride, Dietary ,Iodine - Abstract
Universal salt iodization is considered the best strategy for controlling iodine deficiency disorders in Nepal. This study was done to find iodized salt use among Nepalese population and the iodine content of household salts.Six districts (Siraha, Saptari, Jhapa, Udayapur, Ilam and Panchthar) were chosen randomly from 16 districts of eastern Nepal for the study. In each district, three schools (private and government) were chosen randomly for sample collection. A total of 1803 salt samples were collected from schools of those districts. For sample collection a clean air tight plastic pouch was provided to each school child and was asked to bring approximately 15 gm of their kitchen salt. The information about type of salt used; 'two child logo' iodized salt or crystal salt was obtained from each child and salt iodine content was estimated using iodometric titration.At the time of study, 85% (n=1533) of Nepalese households were found to use iodized salt whereas 15% (n=270) used crystal salt. The mean iodine content in iodized and crystal salt was 40.8±12.35 ppm and 18.43±11.49 ppm respectively. There was significant difference between iodized and crystal salts use and salt iodine content of iodized and crystal salt among different districts (p value0.001 at confidence level of 95%). Of the total samples, only 169 samples (9.4% of samples) have iodine content15 ppm.Most Nepalese households have access to iodized salt most salt samples have sufficient iodine content.
8. Prevalence and antimicrobial susceptibility of Acinetobacter from raw bulk tank milk in Korea.
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Gurung, M., Nam, H. M., Tamang, M. D., Chae, M. H., Jang, G. C., Jung, S. C., and K. Lim, S.
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GRAM-negative bacteria , *MILK microbiology , *MILK analysis , *ANTI-infective agents , *FOOD pathogens , *PUBLIC health - Abstract
The objective of this study was to determine the prevalence of Acinetobacter spp. in bulk tank milk (BTM) samples from different provinces of Korea and to analyze their antimicrobial susceptibility. Altogether, 2,287 BTM samples were investigated. Among them, Acinetobacter spp. were isolated from 176 BTM samples. Out of 176 Acinetobacter spp., 57 isolates were identified as Acinetobacter baumannii. None of the isolates were resistant to cefepime, imipenem, meropenem, ciprofloxacin, levofloxacin, or colistin. Resistance to amikacin, gentamicin, piperacillin, and cefotaxime was 2.3, 7.4, 2.3, and 4.0%, respectively. Acinetobacter spp. were least susceptible to tetracycline (17.6%), followed by trimethoprim-sulfamethoxazole (15.9%), ceftazidime (10.8%), and ampicillin-sulbactam (10.2%). Overall, A. baumannii strains were susceptible to most of the antimicrobial agents tested compared with other Acinetobacter spp. The Acinetobacter isolates showed 17 different patterns of antimicrobial resistance. The most frequent resistance profile observed was ampicillin-sulbactam (n = 13), followed by tetracycline (n = 9), ceftazidime-tetracycline (n = 8), and trimethoprimsulfamethoxazole- tetracycline (n = 8). The results of this study confirmed that Acinetobacter, including A. baumannii strains, are present in BTM, which clearly showed the importance of examining BTM not only for foodborne pathogens but also for Acinetobacter spp., which could be of public health concern. To the best of our knowledge, this is the first report of Acinetobacter spp. in BTM samples from Korea. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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9. How Low Back Pain is Managed-A Mixed-Methods Study in 32 Countries. Part 2 of Low Back Pain in Low- and Middle-Income Countries Series.
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Sharma S, Pathak A, Parker R, Costa LOP, Ghai B, Igwesi-Chidobe C, Janwantanakul P, de Jesus-Moraleida FR, Chala MB, Pourahmadi M, Briggs AM, Gorgon E, Ardern CL, Khan KM, McAuley JH, Alghwiri A, Aoko OA, Badamasi HS, Calvache JA, Cardosa MS, Ganesh S, Gashaw M, Ghiringhelli J, Gigena S, Hasan AT, Haq SA, Jacob EN, Janse van Rensburg DC, Kossi O, Liu C, Malani R, Mason BJN, Najem C, Nava-Bringas TI, Nduwimana I, Perera R, Perveen W, Pierobon, Pinto E, Pinto RZ, Purwanto F, Rahimi MD, Reis FJ, Siddiq MAB, Shrestha D, Tamang M, Vasanthan T L, and Viljoen C
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- Humans, Female, Male, Adult, Middle Aged, Self-Management, Surveys and Questionnaires, Low Back Pain therapy, Developing Countries
- Abstract
BACKGROUND: The Lancet Low Back Pain (LBP) Series highlighted the lack of LBP data from low- and middle-income countries (LMICs). The study aimed to describe (1) what LBP care is currently delivered in LMICs and (2) how that care is delivered. DESIGN: An online mixed-methods study. METHODS: A Consortium for LBP in LMICs (n = 65) was developed with an expert panel of leading LBP researchers (>2 publications on LBP) and multidisciplinary clinicians and patient partners with 5 years of clinical/lived LBP experience in LMICs. Quantitative data were analyzed using descriptive statistics. Two researchers independently analyzed qualitative data using inductive and deductive coding and developed a thematic framework. RESULTS: Forty-seven (85%) of 55 invited panel members representing 32 LMICs completed the survey (38% women, 62% men). The panel included clinicians (34%), researchers (28%), educators (6%), and people with lived experience (4%). Pharmacotherapies and electrophysiological agents were the most used LBP treatments. The thematic framework comprised 8 themes: (1) self-management is ubiquitous, (2) medicines are the cornerstone, (3) traditional therapies have a place, (4) society plays an important role, (5) imaging use is very common, (6) reliance on passive approaches, (7) social determinants influence LBP care pathway, and (8) health systems are ill-prepared to address LBP burden. CONCLUSION: LBP care in LMICs did not consistently align with the best available evidence. Findings will help research prioritization in LMICs and guide global LBP clinical guidelines. J Orthop Sports Phys Ther 2024;54(8):560-572. Epub 11 April 2024. doi:10.2519/jospt.2024.12406 .
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- 2024
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10. Gemykibivirus detection in acute encephalitis patients from Nepal.
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Tuladhar ET, Shrestha S, Vernon S, Droit L, Mihindukulasuriya KA, Tamang M, Karki L, Elong Ngono A, Jha B, Awal BK, Chalise BS, Jha R, Shresta S, Wang D, and Manandhar KD
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- Humans, Nepal epidemiology, Male, Child, Metagenomics, High-Throughput Nucleotide Sequencing, Child, Preschool, Real-Time Polymerase Chain Reaction, Encephalitis, Viral virology, Adolescent, DNA Viruses genetics, DNA Viruses isolation & purification, DNA Viruses classification, Female, Genome, Viral genetics, Phylogeny
- Abstract
Acute encephalitis syndrome (AES) causes significant morbidity and mortality worldwide. In Nepal, Japanese encephalitis virus (JEV) accounts for ~5-20% of AES cases, but ~75% of AES cases are of unknown etiology. We identified a gemykibivirus in CSF collected in 2020 from an 8-year-old male patient with AES using metagenomic next-generation sequencing. Gemykibiviruses are single stranded, circular DNA viruses in the family Genomoviridae . The complete genome of 2,211 nucleotides was sequenced, which shared 98.69% nucleotide identity to its closest relative, Human associated gemykibivirus 2 isolate SAfia-449D. Two real-time PCR assays were designed, and screening of 337 cerebrospinal fluid (CSF) and 164 serum samples from AES patients in Nepal collected in 2020 and 2022 yielded 11 CSF and 1 serum sample that were positive in both PCR assays. Complete genomes of seven of the positives were sequenced. These results identify a potential candidate etiologic agent of encephalitis in Nepal., Importance: Viral encephalitis is a devastating disease, but unfortunately, worldwide, the causative virus in many cases is unknown. Therefore, it is important to identify viruses that could be responsible for cases of human encephalitis. Here, using metagenomic sequencing of CSF, we identified a gemykibivirus in a male child from Nepal with acute encephalitis syndrome (AES). We subsequently detected gemykibivirus DNA in CSF or serum of 12 more encephalitis patients by real-time PCR. The virus genomes we identified are highly similar to gemykibiviruses previously detected in CSF of three encephalitis patients from Sri Lanka. These results raise the possibility that gemykibivirus could be an underrecognized human pathogen., Competing Interests: The authors declare no conflict of interest.
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- 2024
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11. Inguinal Lipoblastoma Masquerading as Obstructed Omentocele in a Girl.
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Sahu A, Tamang M, Tripathy BB, Sahoo SK, Naik D, Manekar AA, and Mohanty MK
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Lipoblastoma in the inguinal regional is a rare occurrence in children and can present as a surgical surprise during pediatric herniotomies. Irreducible inguinal hernia is one of the most common surgical problems dealt by pediatric surgery residents in emergency. We report a case of inguinal lipoblastoma presenting as irreducible groin swelling. Complete excision of the mass was done with no recurrence till date., Competing Interests: There are no conflicts of interest., (Copyright: © 2024 Journal of Indian Association of Pediatric Surgeons.)
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- 2024
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12. Dengue Virus Surveillance in Nepal Yields the First On-Site Whole Genome Sequences of Isolates from the 2022 Outbreak.
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Napit R, Ngono AE, Mihindukulasuriya KA, Pradhan A, Khadka B, Shrestha S, Droit L, Paredes A, Karki L, Khatiwada R, Tamang M, Chalise BS, Rawal M, Jha B, Wang D, Handley SA, Shresta S, and Manandhar KD
- Abstract
Background: The 4 serotypes of dengue virus (DENV1-4) can each cause potentially deadly dengue disease, and are spreading globally from tropical and subtropical areas to more temperate ones. Nepal provides a microcosm of this global phenomenon, having met each of these grim benchmarks. To better understand DENV transmission dynamics and spread into new areas, we chose to study dengue in Nepal and, in so doing, to build the onsite infrastructure needed to manage future, larger studies., Methods and Results: During the 2022 dengue season, we enrolled 384 patients presenting at a hospital in Kathmandu with dengue-like symptoms; 79% of the study participants had active or recent DENV infection (NS1 antigen and IgM). To identify circulating serotypes, we screened serum from 50 of the NS1
+ participants by RT-PCR and identified DENV1, 2, and 3 - with DENV1 and 3 codominant. We also performed whole-genome sequencing of DENV, for the first time in Nepal, using our new on-site capacity. Sequencing analysis demonstrated the DENV1 and 3 genomes clustered with sequences reported from India in 2019, and the DENV2 genome clustered with a sequence reported from China in 2018., Conclusion: These findings highlight DENV's geographic expansion from neighboring countries, identify China and India as the likely origin of the 2022 DENV cases in Nepal, and demonstrate the feasibility of building onsite capacity for more rapid genomic surveillance of circulating DENV. These ongoing efforts promise to protect populations in Nepal and beyond by informing the development and deployment of DENV drugs and vaccines in real time.- Published
- 2024
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13. Perspectives on chronic musculoskeletal pain management among healthcare professionals in Bhutan: A qualitative study.
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Tamang M, Dorji N, Wangdi K, Letho Z, Lhaki K, and Dorji T
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- Humans, Bhutan, Qualitative Research, Attitude of Health Personnel, Musculoskeletal Pain therapy, Chronic Pain therapy, Chronic Pain psychology
- Abstract
Introduction: Musculoskeletal pain disorders continue to be one of the leading causes of disability worldwide. Best practice care recommends patient-centred biopsychosocial models of care. Little is known about the chronic musculoskeletal pain management in low- and middle-income countries., Objectives: To explore the management of chronic musculoskeletal pain by healthcare professionals in Bhutan., Methods: This study employed a qualitative design involving 19 Bhutanese healthcare professionals managing chronic musculoskeletal pain in Bhutan. We collected data using a semi-structured interview guide. Thematic analysis was done manually., Results: Healthcare professionals reported that patients rely on a combination of home remedies and seek help from local healers and hospitals for chronic musculoskeletal pain management. While healthcare professionals understand chronic musculoskeletal pain as multi-dimensional phenomenon, the primary focus was on identifying and treating structural anomalies. Time constraints, inadequate knowledge and skills, lack of confidence, facilities and expertise were reported as barriers to the effective management of chronic musculoskeletal pain. They proposed a need for clinical guidelines, relevant skills training and creating awareness of chronic musculoskeletal pain., Conclusion: The management of chronic musculoskeletal pain in Bhutan is focused on identifying and treating physical abnormalities. Healthcare professionals consider psychosocial dimensions important but lack any form of actionable interventions in this domain., (© 2023 John Wiley & Sons Ltd.)
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- 2023
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14. Parosteal lipoma of rib causing widening of intercostal space in an adolescent.
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Tamang M, Mahalik SK, and Tripathy TP
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- Adult, Child, Humans, Female, Adolescent, Magnetic Resonance Imaging, Ribs diagnostic imaging, Ribs surgery, Ribs pathology, Lipoma diagnostic imaging, Lipoma surgery, Lipoma pathology, Bone Neoplasms diagnostic imaging, Bone Neoplasms surgery, Bone Neoplasms pathology, Thoracic Wall pathology
- Abstract
Parosteal lipomas are typically seen in adults and affect mostly the diaphysis of the long bones of the upper and lower limbs. Only a few cases have been reported in children until now and none were reported involving ribs. A female adolescent patient presented with swelling on the left upper back over the scapular region for the past 4 years without any neurological deficit. MRI revealed a hyperintense lesion on the left paravertebral region extending from D5 to D12 and into the intercostal space, causing it to widen. The patient underwent surgical excision. The lipomatous mass was seen entering in to the intercostal space, causing it to widen. However, the pleura was not breached. In this report, we have performed a literature review compiling all cases of parosteal lipoma affecting the ribs., Competing Interests: Competing interests: None declared., (© BMJ Publishing Group Limited 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
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- 2023
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15. An Innovative Random-Forest-Based Model to Assess the Health Impacts of Regular Commuting Using Non-Invasive Wearable Sensors.
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Sharif MS, Raj Theeng Tamang M, Fu CHY, Baker A, Alzahrani AI, and Alalwan N
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- Adult, Humans, Bayes Theorem, Surveys and Questionnaires, Transportation, Support Vector Machine, Electroencephalography methods, Wearable Electronic Devices
- Abstract
Regular commutes to work can cause chronic stress, which in turn can cause a physical and emotional reaction. The recognition of mental stress in its earliest stages is very necessary for effective clinical treatment. This study investigated the impact of commuting on human health based on qualitative and quantitative measures. The quantitative measures included electroencephalography (EEG) and blood pressure (BP), as well as weather temperature, while qualitative measures were established from the PANAS questionnaire, and included age, height, medication, alcohol status, weight, and smoking status. This study recruited 45 (n) healthy adults, including 18 female and 27 male participants. The modes of commute were bus (n = 8), driving (n = 6), cycling (n = 7), train (n = 9), tube (n = 13), and both bus and train (n = 2). The participants wore non-invasive wearable biosensor technology to measure EEG and blood pressure during their morning commute for 5 days in a row. A correlation analysis was applied to find the significant features associated with stress, as measured by a reduction in positive ratings in the PANAS. This study created a prediction model using random forest, support vector machine, naive Bayes, and K-nearest neighbor. The research results show that blood pressure and EEG beta waves were significantly increased, and the positive PANAS rating decreased from 34.73 to 28.60. The experiments revealed that measured systolic blood pressure was higher post commute than before the commute. For EEG waves, the model shows that the EEG beta low power exceeded alpha low power after the commute. Having a fusion of several modified decision trees within the random forest helped increase the performance of the developed model remarkably. Significant promising results were achieved using random forest with an accuracy of 91%, while K-nearest neighbor, support vector machine, and naive Bayes performed with an accuracy of 80%, 80%, and 73%, respectively.
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- 2023
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16. Situation of physical activity in the prevention of non-communicable diseases in Bhutan: challenges and the way forward.
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Tamang M, Dahal BP, Dorji T, Tamang ST, and Lucero-Prisno DE 3rd
- Abstract
Bhutan is a lower-income-middle-income country in the Himalayas, between India and China. Non-communicable diseases (NCDs) are the leading cause of death and premature mortality in Bhutan, accounting for 69% and 71% of all deaths in 2014 and 2019, respectively. Although the World Health Organisation (WHO) identified physical activity as a key strategy to reduce the burden of NCDs, with rapid urbanisation, motorised transportation, and rural-urban migration, people are adapting to sedentary lifestyles, inflating the incidence of NCDs in the country. The increasing incidence of NCDs exerts a burden on the human and financial resource constraints in the health system in Bhutan. In this view point, we report evidence-based benefits of physical activity for health promotion, primary prevention of NCDs and health benefits for individuals living with NCDs. We also briefly assess the situation of physical activity in Bhutan. Then, shortcomings of existing NCDs prevention programmes are discussed along with opportunities and ways forward to effectively implementing physical activity to harness the benefits of physical activities., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
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- 2022
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17. The Impact of Basic Police Training and Scale Diet on Body Composition and Aerobic Performance of Nepal Police Officers Trainees.
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Rajbhandari B, Pant S, Adhikari B, Gurung M, Poudel L, Shilpakar O, Karki S, Yonzon Tamang M, Khadka P, Shrestha B, Baniya B, Shrestha B, Thakuri BJ, Maharajan A, Singh E, Gajmer B, Singh S, Regmi A, Maskey P, Tiwari B, and Mali A
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- Body Composition physiology, Cross-Sectional Studies, Diet, Humans, Nepal, Physical Fitness physiology, Police
- Abstract
Introduction: Executing their daily duties, police officers are expected to perform complex, physically demanding activities, often at maximal levels of exertion. Given these criteria, training must be rigorous enough to ensure that when trainees graduate, they are competent in their response to crisis and flexible enough to sustain this for the span of their career. The present study aimed to determine the impact of scale diet and physical training (SDPT) on body composition and aerobic performance among Nepal police officer trainees., Method: We conducted a cross-sectional analytic study among 570 Nepal Police recruits from Nepal Police Academy of Butwal and Kathmandu. Null hypothesis was created followed by an alternative hypothesis. Alpha (1 - ?) for the above-mentioned study design were set. Parametric statistical analysis was done with criteria set for acceptance or rejection of the null hypothesis. We collected the data from the field as per Nepal police basic exercise and diet protocol to determine the effect of physical training and scale diet on body composition and aerobic performance. We enrolled all the recruits from March to May, 2019 who met the eligibility criteria in the study. We used Microsoft Excel 2013 for data entry and R program (version: 4.0.3) for statistical analysis. Results:There was a significant rise in aerobic performance (4.51 ± 3.39 ml/kg/min) and remarkable reduction in body fat (-4.49 ± 2.33 %) and Body Mass Index (-0.433 ± 1.00 kg/m2) after SDPT among participants with normal BMI at the time of recruitment. Body fat was significantly reduced (-4.36 ± 2.3%) and aerobic performance was significantly increased (6.93±4.70 ml/kg/min) among overweight participants at the time of recruitment., Conclusions: The methodology adopted in the physical training of Nepal Police was effective in reducing body mass index and fat, improving the physical capacity. . Key words: Scale Diet; Physical Training, Body Fat; Aerobic Performance; VO2max, Body Mass Index.
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- 2022
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18. Advances in treatment of coking wastewater - a state of art review.
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Tamang M and Paul KK
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- Nitrogen, Waste Disposal, Fluid, Wastewater analysis, Coke analysis, Polycyclic Aromatic Hydrocarbons analysis
- Abstract
Coking wastewater poses a serious threat to the environment due to the presence of a wide spectrum of refractory substances such as phenolic compounds, polycyclic aromatic hydrocarbons and heterocyclic nitrogenous compounds. These toxic substances are difficult to treat using conventional treatment methods alone. In recent years much attention has been given to the effective treatment of coking wastewater. Thus, this review seeks to provide a brief overview of recent developments that have taken place in the treatment of coking wastewater. In addition, this article addresses the complexity and the problems associated with treatment followed by a discussion on biological methods with special focus on bioaugmentation. As coking wastewater is refractory in nature, some of the studies have been related to improving the biodegradability of wastewater. The final section focuses on the integrated treatment methods that have emerged as the best solution for tackling the highly unmanageable coking wastewater. Attention has also been given to emerging microwave technology which has tremendous potential for treatment of coking wastewater.
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- 2022
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19. Physiotherapy services in patient care in Bhutan.
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Tamang M and Dorji T
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- Bhutan, Humans, Patient Care, Workforce, Delivery of Health Care, Physical Therapy Modalities
- Abstract
Physiotherapy and rehabilitative services are an integral part of patient care, but in many developing countries they are not considered a priority and are either not available or not easily accessible to those who need them. Bhutan is one such country where healthcare is provided free of cost to all, but as of 2021 physiotherapy services were available only in 26 of 48 hospitals and 19 of 20 districts. The number of physiotherapy professionals per 10,000 population is 1.4 with significant rates of attrition. There is lack of awareness among patients and other health professionals about physiotherapy and rehabilitation services. The country needs to integrate physiotherapy and rehabilitation services into the overall health policy framework and develop proper planning of human resources and infrastructure to meet the current and future demands., (© 2021. The Author(s).)
- Published
- 2021
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20. Perceived fear of COVID-19 and its associated factors among Nepalese older adults in eastern Nepal: A cross-sectional study.
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Yadav UN, Yadav OP, Singh DR, Ghimire S, Rayamajhee B, Kanti Mistry S, Rawal LB, Ali AM, Kumar Tamang M, and Mehta S
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- Acute Disease, Aged, Aged, 80 and over, Cross-Sectional Studies, Female, Health Services Accessibility statistics & numerical data, Humans, Logistic Models, Male, Middle Aged, Nepal epidemiology, SARS-CoV-2 pathogenicity, Stress, Psychological physiopathology, COVID-19 epidemiology, COVID-19 psychology, Fear psychology, Pandemics, Stress, Psychological epidemiology
- Abstract
Background: Coronavirus disease 2019 (COVID-19) has affected all age groups worldwide, but older adults have been affected greatly with an increased risk of severe illness and mortality. Nepal is struggling with the COVID-19 pandemic. The normal life of older adults, one of the vulnerable populations to COVID-19 infection, has been primarily impacted. The current evidence shows that the COVID-19 virus strains are deadly, and non-compliance to standard protocols can have serious consequences, increasing fear among older adults. This study assessed the perceived fear of COVID-19 and associated factors among older adults in eastern Nepal., Methods: A cross-sectional study was conducted between July and September 2020 among 847 older adults (≥60 years) residing in three districts of eastern Nepal. Perceived fear of COVID-19 was measured using the seven-item Fear of COVID-19 Scale (FCV-19S). Multivariate logistic regression identified the factors associated with COVID-19 fear., Results: The mean score of the FCV-19S was 18.1 (SD = 5.2), and a sizeable proportion of older adults, ranging between 12%-34%, agreed with the seven items of the fear scale. Increasing age, Dalit ethnicity, remoteness to the health facility, and being concerned or overwhelmed with the COVID-19 were associated with greater fear of COVID-19. In contrast, preexisting health conditions were inversely associated with fear., Conclusion: Greater fear of the COVID-19 among the older adults in eastern Nepal suggests that during unprecedented times such as the current pandemic, the psychological needs of older adults should be prioritized. Establishing and integrating community-level mental health support as a part of the COVID-19 preparedness and response plan might help to combat COVID-19 fear among them., Competing Interests: The authors have declared that no competing interests exist.
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- 2021
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21. Short communication: Genetic characterization of antimicrobial resistance in Acinetobacter isolates recovered from bulk tank milk.
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Tamang MD, Gurung M, Nam HM, Kim SR, Jang GC, Jung SC, and Lim SK
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- Acinetobacter metabolism, Acinetobacter baumannii genetics, Acinetobacter baumannii metabolism, Animals, Bacterial Proteins metabolism, Electrophoresis, Gel, Pulsed-Field, Integrons, Polymerase Chain Reaction, Republic of Korea, Acinetobacter genetics, Anti-Bacterial Agents pharmacology, Bacterial Proteins genetics, Drug Resistance, Bacterial, Genetic Variation, Milk microbiology
- Abstract
A total of 176 Acinetobacter isolates, including 57 Acinetobacter baumannii originally obtained from 2,287 bulk tank milk (BTM) samples in Korea was investigated for the genetic basis of antimicrobial resistance using molecular methods. In addition, the occurrence and cassette content of integrons were examined and the genetic diversity of A. baumannii strains identified was evaluated. Aminoglycoside-modifying enzyme genes were detected in 15 (88.2%) of the 17 aminoglycoside-resistant Acinetobacter isolates tested. The most common aminoglycoside-modifying enzyme gene identified was adenylyltransferase gene aadB (n = 9), followed by phosphotransferase genes aphA6 (n = 7) and aphA1 (n = 5). Of the 31 isolates resistant to tetracycline, tet(39) was detected in 20 of them. The genetic basis of resistance to sulfonamide was identified in 15 (53.6%) of 28 trimethoprim-sulfamethoxazole-resistant isolates and 9 (32.1%) of them carried both sul1 and sul2 genes. A blaADC-7-like gene was detected in 1 β-lactam-resistant A. baumannii. Furthermore, class 1 integron was identified in 11 Acinetobacter isolates. Two gene cassettes dfrA15, conferring resistance to trimethoprim, and aadA2, conferring resistance to aminoglycosides, were identified in 8 Acinetobacter isolates. None of the isolates was positive for class 2 or class 3 integrons. Pulsed-field gel electrophoresis revealed that most of the A. baumannii strains from BTM samples were genetically diverse, indicating that the occurrence of A. baumannii strains in BTM was not the result of dissemination of a single clone. Elucidation of resistance mechanisms associated with the resistance phenotype and a better understanding of resistance genes may help in the development of strategies to control infections, such as mastitis, and to prevent further dissemination of antibiotic resistance genes. To the best of our knowledge, this is the first report of molecular characterization of antimicrobial-resistant Acinetobacter spp. from milk., (Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
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- 2014
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22. Characterization of Candida species isolated from cases of lower respiratory tract infection.
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Jha BJ, Dey S, Tamang MD, Joshy ME, Shivananda PG, and Brahmadatan KN
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- Adult, Age Distribution, Aged, Aged, 80 and over, Candida classification, Candida isolation & purification, Candidiasis epidemiology, Diabetes Complications complications, Female, Hospitals, Teaching, Humans, Incidence, Lung Diseases, Fungal epidemiology, Male, Malnutrition complications, Middle Aged, Mycological Typing Techniques, Neoplasms complications, Nepal epidemiology, Population Surveillance, Pulmonary Disease, Chronic Obstructive complications, Risk Factors, Sex Distribution, Smoking adverse effects, Sputum microbiology, Tuberculosis, Pulmonary complications, Candidiasis microbiology, Lung Diseases, Fungal microbiology
- Abstract
Objectives: (1) To identify and characterize the Candida species isolates from lower respiratory tract infection. (2) to determine the rate of isolation of Candida species from sputum samples., Methods: This study was carried out in the Department of Microbiology, Manipal Teaching Hospital, Pokhara, Nepal from June 2002 to January 2003. A total of 462 sputum samples were collected from patients suspected lower respiratory tract infection. The samples were processed as Gram staining to find out the suitability of the specimen, cultured on Sabouraud's Dextrose Agar (SDA) and also on blood agar and chocolate agar to identify the potential lower respiratory tract pathogens. For the identification of Candida, sputum samples were processed for Gram stain, culture, germ tube test, production of chlamydospore, sugar fermentation and assimilation test. For the identification of bacteria, Gram stain, culture, and biochemical tests were performed by standardized procedure., Result: Out of 462 samples, 246 (53.24%) samples grew potential pathogens of lower respiratory tract. Among them Haemophilus influenzae 61(24.79%) and Streptococcus pneumoniae 57 (23.17%) were the predominant bacterial pathogens. Candida species were isolated from 30 samples (12.2%). The majority of Candida species amongst the Candida isolates were Candida albicans 21(70%) followed by Candida tropicalis 4(13.33%). Candida krusei 3(10%), Candida parapsilosis 1(3.33%) and Candida stellatoidea 1(3.33%). The highest rate of isolation of Candida was between the age of 71 and 80., Conclusion: Candida isolation from sputum samples is important as found in the present study in which Candida species were the third most common pathogen isolated from patients with lower respiratory tract infection.
- Published
- 2006
23. Prevalence of Helicobacter pylori in Dhulikhel Hospital, Kathmandu University Teaching Hospital: a retrospective histopathologic study.
- Author
-
Makaju RK, Tamang MD, Sharma Y, Sharma N, Koju R, and Ashraf M
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Chronic Disease, Female, Gastritis diagnosis, Gastritis epidemiology, Helicobacter Infections diagnosis, Humans, Male, Middle Aged, Nepal epidemiology, Prevalence, Gastritis microbiology, Helicobacter Infections epidemiology, Helicobacter pylori
- Abstract
Objective: The main objective of this study was to determine prevalence of Helicobacter pylori infection in Dhulikhel Hospital, Kathmandu University Teaching Hospital., Methodology: Endoscopic antral biopsies from January 1, 2004 to August 31, 2005 were studied retrospectively. Hematoxylin and eosin and Giemsa stained histological sections were examined., Results: Out of 224 patients (Male 125 and female 99) who underwent endoscopic biopsy and included in the study, a total of 76 (33.9%) patients (Male 50 and female 26) were infected by H. pylori. The mean age of the H. pylori infected patients was 40.2 years (SD 16.0). The infection by H. pylori was significantly higher in males than females (p<0.05) with male to female ratio of 1.9:1. The most common (31.3%) histopathologic finding was chronic superficial gastritis followed by normal histology (30.8%). A total of 10 cases (4.5%) of adenocarcinoma and 16 cases (7.1%) of intestinal metaplasia were detected and neither of them showed presence of H. pylori. Two cases (0.9%) were diagnosed as suspected malignancy and both were negative for H. pylori infection. Among gastritis, H. pylori was most frequently observed in chronic active gastritis (86.1%). Two of 69 cases (2.9%) of normal gastric mucosa showed H. pylori.
- Published
- 2005
24. An outbreak of El Tor cholera in Kavre district, Nepal.
- Author
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Tamang MD, Sharma N, Makaju RK, Sarma AN, Koju R, Nepali N, and Mishra SK
- Subjects
- Adolescent, Adult, Age Distribution, Aged, Child, Comorbidity, Female, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Nepal epidemiology, Parasitic Diseases epidemiology, Prospective Studies, Cholera epidemiology, Disease Outbreaks, Vibrio cholerae O1 drug effects
- Abstract
Outbreak of cholera still remains major public health problem in most of the developing countries including Nepal. A prospective study was carried out at Dhulikhel Hospital, Kathmandu University Teaching Hospital, Kavrepalanchok during 1st May 2004 to 31st October 2004. A total of 148 stool samples from patients with acute diarrhea were collected and further investigated for cholera. The study was conducted to establish the causes of the outbreak of acute diarrheal disease, antimicrobial profiles of the stool isolates and parasitic co-infection in cholera cases. The samples were subjected to standard recommended microbial procedures and confirmation of the isolates was done by seroagglutination using V.cholerae polyvalent O1 and 0139 antisera and monovalent Ogawa and Inaba antisera. Out of the 148 stool samples, 46 cases (31%) were found to be positive for V.cholerae serogroup O1, biotype ElTor, serotype Ogawa. Both sexes were equally affected. Young age group of less than 30 years were mostly affected. Brahmin was the most affected ethnic group. The isolates were sensitive to all the antibiotics tested except co-trimoxazole. Among the laboratory confirmed cholera cases 30% exhibited co-infection with other parasites among which Giardia lamblia and Ascaris lumbricoides were the most common.
- Published
- 2005
25. Prevalence of Moraxella catarrhalis infections of the lower respiratory tract in elderly patients.
- Author
-
Tamang MD, Dey S, Makaju RK, Jha BK, Shivananda PG, and Bhramadatan KN
- Subjects
- Age Distribution, Aged, Aged, 80 and over, Anti-Bacterial Agents pharmacology, Disease Susceptibility, Drug Resistance, Bacterial, Female, Humans, Male, Middle Aged, Moraxellaceae Infections drug therapy, Nepal epidemiology, Prevalence, Prospective Studies, Respiratory Tract Infections drug therapy, Respiratory Tract Infections microbiology, Seasons, Sex Distribution, Moraxella catarrhalis isolation & purification, Moraxellaceae Infections epidemiology, Respiratory Tract Infections epidemiology
- Abstract
Objectives: To determine: *rate of isolation of Moraxella catarrhalis in elderly patients having lower respiratory tract infection *The antibiotic susceptibility pattern of Moraxella catarrhalis isolates. *The predisposing factors responsible for Moraxella catarrhalis infections of lower respiratory tract., Material and Method: This is a prospective study carried out at Manipal Teaching Hospital, Pokhara during May 2002 to April 2003. A total of 716 sputum samples collected from patients with suspected lower respiratory tract infection were investigated. The samples were subjected to Gram stain, culture and sensitivity. Sputum samples were inoculated on blood and chocolate agar and incubated at 37 degrees C for 18 to 24 hours in candle jar containing 3-5% Co2. For the identification of bacterial isolates, the cultural and colonial characteristics were recorded and identified with the use of biochemical test. The susceptibility of bacterial isolates to antimicrobial agent was performed following Kirby-Bauer method., Result: Out of the 716 samples, 355 (49.58%) grew normal commensals of the upper respiratory tract. Respiratory pathogens were recovered from 361 (50.41%) samples. The most common respiratory pathogen was H. influenzae (26. 86%), followed by S. pneumoniae (21.16%). Moraxella catarrhalis accounted for 6.90%. Rate of isolation of Moraxella catarrhalis was higher in males than in females. Out of 25 isolates 16 were from males and 9 were from females. Frequency of isolation of Moraxella catarrhalis was more frequently seen in age group 61-70 years. It was most commonly recovered in winter. The most effective antibiotics were amoxicillin-clavulanate and ceftriaxone and least was ampicillin., Conclusion: Moraxella catarrhalis isolation from sputum especially in persons above 60 years of age and in the absence of other well established pathogens should not be disregarded as they can cause lower respiratory tract infection in these individuals.
- Published
- 2005
26. Typhoid fever in Dhulikhel hospital, Nepal.
- Author
-
Sharma N, Koju R, Karmacharya B, Tamang MD, Makaju R, Nepali N, Shrestha P, and Adhikari D
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents therapeutic use, Child, Female, Humans, Male, Middle Aged, Nepal, Typhoid Fever drug therapy, Typhoid Fever diagnosis
- Abstract
One hundred and twelve cases of typhoid fever presenting in outpatient and emergency department of Dhulikhel Hospital in Nepal were studied. In this study, it was found that 71% typhoid fever cases were less than 30 years of age group with male to female ratio of 3:1. Fever over 5 days followed by headache and chills were major presenting symptoms. Widal test and blood culture for Salmonella typhi were positive in 59% and 49% cases respectively. Two third of our study population had total leucocyte count of normal range. The fever clearance time was significantly better with ofloxacin compared to ciprofloxacin (p < 0.05) and ceftriaxone compared to chloramphenicol (p < 0.05). The release from treatment was significantly shorter with ceftriaxone compared to ofloxacin, ciprofloxacin and chloramphenicol (p < 0.01). Ceftriaxone was found to be 100% sensitive to salmonella typhi. Amoxicillin was only 52.1% sensitive to Salmonella typhi. Early diagnosis and institution of appropriate antibiotic therapy is of paramount importance in the management of typhoid patients.
- Published
- 2004
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