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Mosaic loss of chromosome Y (mLOY) in leukocytes has attracted much attention as an emerging biomarker of aging and aging-related diseases. We evaluated the usefulness of saliva for mLOY analysis and showed that saliva-derived mLOY is significantly associated with aging and increased physical activity, but not with smoking. While these data support the robust association between saliva-derived mLOY and aging, caution is required when comparing data from saliva-derived and blood-derived mLOY.

This study investigated oleate inhibition concentration on mesophilic and thermophilic sludge by utilizing acetate and H2/CO2 (80:20, v/v) as substrate, respectively. In addition, another batch experiment was carried out to explore the influence of oleate loads (mM-oleate/g-VS) on methane production. Generally, the mesophilic anaerobic system was more stable than the thermophilic system, which embodied higher microbial abundance, higher methane yield, and higher oleate tolerance. Furthermore, this study provides a possible methanogenic pathway impacted by oleate under mesophilic and thermophilic conditions according to functional microbial composition. Lastly, this paper provides noticeable and avoidable oleate concentrations and loads under different experimental conditions as a guide for future anaerobic bioreactors of lipidic waste biodegradation.

This study investigated the effects of the addition of micro- (Fe, Co, Ni, and Mo) and macro-(Sulfur) nutrients on mono-digestion of sorghum under mesophilic conditions. A continuous stirred-tank reactor was operated for more than 420 days under seven different experimental conditions. The experimental results showed poor performance for methane production and process stability without nutrient supplementation. Serious deficiencies in Co and S were confirmed by nutrient analysis of dry sorghum and digestate. Nutrient augmentation efficiently enhanced methane production and volatile fatty acid (VFA) removal. Methane production reached 223 mL-CH4/g-VS, almost matching the yield predicted by biochemical methane potential (BMP) test. S was demonstrated to have a critical effect on metal availability in the digester. Consequently, to maintain stable methane fermentation, suitable supplementations of S and Co are recommended for anaerobic sorghum mono-digestion.

Engineered biochar production and utilization in anaerobic digestion (AD) potentially overcome its limited application to the treatment of slowly degradable or inhibitory substrates. Here an attempt was made to develop an optimized biochar production procedure for use in AD to stimulate palmitic acid biodegradation via direct interspecies electron transfer (DIET). The electrical conductivity of biochar was greatly increased with an elevated pyrolysis temperature and K

Pelvic bottom dysfunction includes sexual dysfunction, lower urinary tract dysfunction, defecation dysfunction, etc., and the quality of daily life is significantly impaired. Although drug based and surgical therapies exist as treatment methods, non-invasive treatment methods for pelvic floor dysfunction are highly desired, and magnetic stimulation therapy is attracting attention as a potential new approach. Magnetic stimulation therapy can generate deeper stimulations as compared to electrical stimulation therapy, is less painful, and can be performed while wearing clothes. In addition, it is a very safe treatment method with only few reports of side effects. From nocturnal enuresis in children to middle-aged sexual dysfunction and urinary incontinence in the elderly, therapeutic effects on various pelvic floor dysfunctions have been confirmed regardless of age and gender. It is expected that magnetic therapy will continue to develop as a new therapy in the futures. This chapter first describes the pelvic floor muscles and the principles of anatomy and magnetic therapy. In addition, the therapeutic effects of magnetic therapy will be explained in detail one by one. We will also explain the potential application of magnetic therapy for sarcopenia, which is a problem in our aging society.

Oily organic waste is a promising feedstock for anaerobic co-digestion. Free long-chain fatty acids (LCFAs) produced from lipids can inhibit methanogenic consortia, so optimal control of LCFA concentration is the key to successful operation of co-digestion. Most LCFAs are present in the solid phase, making them difficult to be detected and monitored. This study proposes a simple and easy method for detecting LCFAs in both the liquid and solid phases of anaerobic digestate by combining liquid-liquid extraction followed by solid-phase extraction (SPE) and spectrophotometric analysis. The extraction procedure successfully removed impurities that interfere with the absorbance spectrum and ensured high recovery rates of LCFAs. The utility of the pretreatment used for the extraction was discussed using thermodynamic analysis and calculations of phase equilibrium for the solvent extraction system. The absorbance spectrum shift of pyridinium N-phenolate betaine (PNPB) dye-stained solution showed a good correlation with LCFA concentration and enabled highly sensitive measurements. Good quantification was demonstrated in experiments using various digestate samples obtained from the laboratory, pilot, and full-scale reactors.

Tar generated as a by-product during biomass gasification contains a high concentration of refractory organic matters. In this study, a hybrid upflow anaerobic sludge-biochar bed reactor was established for tar treatment, and the methane yield was 120-154 NmL-CH

In this study, an integrated system of siphon-driven self-agitated anaerobic reactor (SDSAR) and anaerobic fixed bed reactor (AFBR) was conducted for the treatment of wastewater from food waste disposer (FWD), and the effect of influent total solids (TS) concentration on the process performance was evaluated. When the influent TS concentration increased from 7.04 to 15.5 g/L, the methane gas production rate increased from 0.45 to 0.92 L-CH4/L/day. However, with the influent TS concentration of food waste (FW) further increased to 23.5 g/L, a large amount of scum formed and accumulated in the SDSAR. According to the result of chemical oxygen demand (COD) recovery, the proportion of COD remained in the effluent at different TS concentrations was only around 2%. On the other hand, with an increase in TS concentration, the proportion of COD remained in the reactors increased significantly. Our results demonstrated that effluent from the integrated system can meet the water quality requirements recommended by Japan Sewage Works Association (JSWA) for wastewater from FWD. In addition, to enhance the process stability, the influent TS concentration should be maintained below 15.5 g/L.

Given the aggravated greenhouse effect caused by CO2 and the current energy shortage, CO2 capture and reuse has been gaining ever-increasing concerns. Microbial Electrolysis Cells (MECs) has been considered to be a promising alternative to recycle CO2 bioelectrochemically to low-carbon electrofuels such as CH4 by combining electroactive microorganisms with electrochemical stimulation, enabling both CO2 fixation and energy recovery. In spite of the numerous efforts dedicated in this field in recent years, there are still many problems that hinder CO2 bioelectroconversion technique from the scaling-up and potential industrialization. This review comprehensively summarized the working principles, extracellular electron transfers behaviors, and the critical factors limiting the wide-spread utilization of CO2 electromethanogenesis. Various characterization and electrochemical testing methods for helping to uncover the underlying mechanisms in CO2 electromethanogenesis have been introduced. In addition, future research needs for pushing forward the development of MECs technology in real-world CO2 fixation and recycling were elaborated.

It is becoming increasingly urgent to investigate the partition coefficients (expressed as log KDOC values) of polybrominated diphenyl ethers (PBDEs) in dissolved organic carbon (DOC) present in wastewater. In the current study, after 72 h of equilibration, the concentrations of four common PBDEs were measured in the presence of four DOC solutions from two laboratories and two full-scale anaerobic digestion plants. Sixteen log KDOCs were determined by calculation and unit conversion. The results for the laboratory samples, such as log KDOCs for 2,2′,4,4′,5,5′-hexabromodiphenyl ether being 6.38 and 5.46 at different reaction temperatures during the cultivate procedure, suggest that a thermophilic environment promotes the solubility of PBDEs to a greater extent than mesophilic conditions. DOC composition directly influences the solubility of PBDEs, even at the same cultivating temperature: the highest log KDOCs for 2,2′,4,4′,5,6′-hexabromodiphenyl ether were 6.71 and 6.33 in different full-scale plant digestates. A linear regression with an R2 of 0.9863 was used to construct a model describing the potential relationship between log KDOC and the composition of DOC, which includes proteins, polysaccharides and lipids, and which takes into account the positions of bromine atoms, for use in predicting the log KDOC values of PBDEs in different water systems.

Poor processing stability has been cited as the fatal shortcoming of the up-flow anaerobic sludge blanket (UASB) reactor treating starch wastewater (SW). In this study, the SW treatment performance in a one-stage UASB reactor and a pre-acidification equipped UASB process were evaluated together with the microbial dynamics. The results revealed that the pre-acidification provided improvements in terms of the substrate utilization diversity and the stability of the microbial community structure on the UASB reactor. Anaerolineaceae/Methanosaeta was the core functional microbiota in the pre-acidification equipped UASB reactor, indicated the superior abilities on the acetogenic methanogenesis of granules. The genus of Methanobacterium, a hydrogenotrophic methanogen was dominant in the archaeal community in the one-stage UASB reactor. The granules performed very strong hydrogen affinity in methane production, a small amount of propionate was detected in the effluent. These were abnormal, which suggested the high hydrogen turn-over rate in the one-stage UASB reactor.

Highlights • Rick husk biochar addition mitigated the pH decrease during high load sorghum AD. • 20 g/L biochar addition reduced the peak total VFA by 375 mg/L at F/M ratio of 1.2. • Maximum methane production rate was increased by 25 % with 15 g/L biochar addition. • Lag phase time was decreased by 45 % with 15 g/L biochar addition., Biochar is a carbon rich product made from the biomass pyrolysis process. Recently, biochar addition in anaerobic digestion processes has attracted attention for its possible functions to act as pH stabilizing agent, microbial carrier, and interspecies electron transfer. In this study, the effects of rice husk biochar addition in sorghum anaerobic digestion were investigated in batch tests. Under high F/M (food to microorganism) ratio conditions, obvious pH decreases and volatile fatty acids (VFA) accumulation was observed. Addition of 15 g/L biochar was found effective to increase the sorghum maximum methane production rate by 25 % and shorten the lag phase time by 44 %. Further biochar concentration increase showed little effects. Alkalinity increase brought by biochar addition contributed to the performance enhancement. Moreover, the promotive effects of biochar addition on degradation of acetic acid, propionic and butyric acid were not obvious under neutral pH conditions.

This study assessed the impact of swine manure (SM) dilution ratio on the microalgal biomass cultivation and further tested for biohydrogen production efficiency from the mixed microalgal biomass. At first, various solid/liquid (S/L) ratio of the SM ranged from 2.5 to 10 g/L was prepared as a nutrient medium for the algal biomass cultivation without addition of the external nutrient sources over a period of 18 d. The peak biomass concentration of 2.57 ± 0.03 g/L was obtained under the initial S/L loading rates of 5 g/L. Further, the cultivated biomass was subjected to two-step (ultrasonication + enzymatic) pretreatment and evaluated for biohydrogen production potential. Results showed that the variable amount of hydrogen production was observed with different S/L ratio of the SM. The peak hydrogen yield of 116 ± 6 mL/g TSadded was observed at the 5 g/L grown SM mixed algal biomass.

This study assessed the co-digestion of macro and microalgal biomass towards the improvement of hydrogen production. The red macroalgal biomass (Gelidium amansii) and green mixed microalgal biomass was mixed in a ratio of 8:2, with an initial substrate concentration of 10 g/L, and various amount of inoculum addition range from 3 to 15% (v/v) was evaluated to assess the feasible substrate to inoculum ratio for the effective co-digestion of the algal biomass. The results showed that the co-digestion with 6% inoculum addition provided the peak hydrogen yield of 45 mL/g dry biomass added with a high hydrogen content of 24% in the gas phase. The other tested conditions showed moderate hydrogen content in the range of 17–22%, respectively. These results suggest that anaerobic co-digestion of macro and microalgal biomass, with appropriate initial biomass loading (6%) is essential for enhanced hydrogen production.

This study investigated early stages of biofilm formation from sieved fine particles of anaerobic granules in the presence of various cationic substances using a quartz crystal sensor to improve biofilm formation in the anaerobic treatment of saline wastewater. The biomass attached on the sensor was greatly increased with Ca within the low range (8–16 mM), which was not affected by 50 mM of Na. However, the positive effect of 16 mM of Ca was strongly reduced in the co-presence of Ca and Na when Na concentrations were in the range from 25 to 150 mM because Ca may compete with Na for the limited binding sites in biofilm. The addition of cationic polymer at 150 mM of Na increased biomass adhesion by several folds at only 10–80 mg/L compared to the addition of 16 mM of Ca. Moreover, no methanogenic inhibition was presented below the polymer content of 20 mg/L.

Dewatering of waste activated sludge (WAS) is of major interest in its volume reduction, transportation and ultimate disposal. Persulfate-based oxidation process is a newly developed option for enhancing WAS dewaterability through the generation of powerful sulfate radicals (SO4−·). However, the enhancement in WAS dewaterability by persulfate differs with the species of iron catalysts used. In this study, two types of iron catalysts (i.e. Fe2+ vs. Fe0) were employed to initiate the persulfate (S2O82−), and the catalyzing behaviors and the underlying principles in enhancing WAS dewaterability were investigated and compared. The Fe2+ exhibited the high effectiveness in catalyzing the decomposition of persulfate to sulfate radicals (SO4−·), inducing the greater improvement in WAS dewatering. The WAS dewaterability (indicated by dry solids content after filtration) increased with the added S2O82−/Fe2+ dosages, with the dry solids content reaching up to 5.1 ± 0.8 wt% at S2O82−/Fe2+ dosages of 1.2/1.5 mmol/g-VS after only 30 s’ filtration, roughly 1.8-fold increase than raw WAS (1.8 ± 0.1 wt%). In contrast, the influence of the persulfate oxidation when activated with Fe0 on WAS dewaterability was statistically insignificant. The WAS dewaterability remained nearly unchanged (i.e. dry solids content of 2.0 ± 0.0 wt%), irrespective of the employed S2O82−/Fe0 dosages. Further analysis demonstrated that the WAS dewaterability negatively corresponded to loosely bound extracellular polymeric substances (LB-EPS) and tightly bound EPS (TB-EPS). The abundant SO4−· from S2O82−/Fe2+ system could effectively disrupt the gel-like EPS matrix, break apart the cells and subsequently arouse the release of the water inside EPS and cells, facilitating water-solid separation. In the case of S2O82−/Fe0, the dissolution of Fe0 particles was the rate-limiting step, due to the formation of oxide iron layer near Fe0 metallic surface, which resulted in the slow SO4−· production and thus hardly promoted WAS dewaterability. The pH adjustment could accelerate Fe0 dissolution and enhance the dewatering performance of S2O82−/Fe0 process to a certain degree, but the effect was unsatisfactory. Additionally, the observations regarding the dissolved organic matters and ammonium collectively revealed that except for enhancing WAS dewatering, S2O82−/Fe2+ oxidation could concurrently degrade COD and ammonia from WAS filtrate, lighten the burden of the subsequent sewage treatment facilities and reduce operational expense. Hence, from an environmental and economic perspective, the S2O82−/Fe2+ system possesses much greater promise for WAS dewatering.

In order to boost the economics of biogas utilization in those small facilities for food waste (FW) digestion, proximate grease trap waste (GTW) was employed to co-digest with FW. A bench-scale continuous stirred tank reactor was set up and operated at mesophilic temperature to investigate the co-digestion limit and lipid/long chain fatty acid (LCFA) conversion by increasing lipid loading stepwise. Mixing FW and GTW at lipid/total solids (TS) 55%, where the lipid loading was 1.61 g/(L·d), was proved to be appropriate, and the maximum methane yield reached 68% higher than the mono-digestion of FW, with a similar lipid reduction at approximately 90%. In addition, since acetic acid accumulation above 100 mg HAc/L had been observed under the optimal loading, the threshold value for anaerobic co-digestion of FW and GTW was confined to the lipid loading around 1.61 g/(L·d). The rapid recovery strategy of mixing the inhibited sludge with fresh inoculum at 1:4 (V/V) took effect after process failure resulted from further increasing lipid/TS to 70%. Lipid/LCFA concentrations in the effluent rose with the increased lipid loading, while palmitic acid accounted for the most percentage among the residual LCFAs, as high as 73.7% at lipid/TS 55%. Acetoclastic methanogens played an important role in accelerating process conversion, due to the fact that raising GTW addition led to the increased methanogenic activity, with a value of 26.9 mL-CH4/(g-VS·d) at the optimal loading.

The effects of organic loading rate (OLR) and operating temperature on the performance of siphon-driven self-agitated anaerobic reactor (SDSAR) in an on-site food waste (FW) treatment system were investigated. Two reactors were operated in parallel for comparison between mesophilic condition (35 ± 1 °C) and thermophilic condition (55 ± 1 °C). With HRT above 15 d and OLR below 4.8 kg-COD/m3/d, relatively high COD removal in the range of 84.5–92.3% was obtained in both reactors. The limits of the loading capacity of the mesophilic SDSAR were observed when OLR was further increased to 7.3 kg-COD/m3/d by shortening HRT. Blocking and gas production reduction occurred and COD removal decreased sharply to 75.9% in the mesophilic reactor. In contrast, the thermophilic reactor can be operated at this OLR with satisfactory COD removal and biogas production. Furthermore, at OLR of 14.4 kg-COD/m3/d, the COD removal was maintained as high as 87.5% in the thermophilic reactor. The conversion of influent COD to methane was maintained above 80% at all the OLR applied in both reactors. The results of this study indicated that thermophilic SDSAR is preferred for the on-site FW treatment.

Low-quality waste oils and fats such as the oily content of trap grease offer an alternative to fossil fuel oil, but they are not fully utilized since they contain saturated fatty acids (SFAs) and saturated triglycerides (STGs), which have relatively high melting points. For practical use of the oily content, it is necessary to remove the components that are solid at room temperature. In this study, we measured the liquidus curves of systems comprising an SFA (stearic acid, palmitic acid, or myristic acid) or an STG (tristearin, tripalmitin, or trimyristin) plus fuel oil A by differential scanning calorimetry. At the same mass fraction of the solid component, the STG plus fuel oil A systems had higher liquidus temperatures than the SFA plus fuel oil A systems with the same fatty acid chains. In both of the SFA plus fuel oil A systems and the STG plus fuel oil A systems, the system in which the solid component had a longer carbon chain had a higher liquidus temperature. On the assumption that fuel oil A is a pseudo-pure component, we proposed two simple methods for predicting liquidus temperature, from the ideal solution approximation and the Dortmund-UNIFAC model, and evaluated their prediction performance by comparing the experimental liquidus curves and the calculated results.

In this pilot-scale study, an innovative mainstream treatment process that couples the anaerobic membrane reactor (AnMBR) with a one-stage PN/A system was proposed for advancing the concept of carbon neutrality in the municipal wastewater treatment plant. This work demonstrates the start-up procedure of a pilot-scale one-stage PN/A system for mainstream treatment. The 255-day start-up of the one-stage PN/A system involved the cultivation of ammonium-oxidizing bacteria (AOB) from the activated sludge, suppression of nitrite-oxidizing bacteria (NOB), investigation of in-situ growth kinetics of anammox bacteria (AnAOB), and the 50-day operation of the pilot-scale AnMBR-PN/A process for natural mainstream treatment. It is verified in the pilot-scale system for the first time that the in-situ free ammonia (FA) and free nitrous acid (FNA) exposure could effectively eliminate the Nitrospira (the NOB genus) while retaining the Nitosonomas (the AOB genus) community in the suspended sludge. NOB community rebounding was not detected even at the mainstream conditions with low nitrogen concentrations (Influent ammonium concentration=38±6 mg-NH4+-N/L) by intermittent aeration to control the system dissolved oxygen (DO) below 0.5 mg/L. The results of the mainstream treatment showed that the average effluent total nitrogen (TN) in the coupled process was generally lower than 10 mg-N/L, which meets the discharge limits of most prefectures in Japan. The investigated results of the in-situ anammox bacteria (AnAOB) growth kinetics suggested that the promoted start-up strategy of taking advantage of the warm months with higher mainstream temperature to achieve the rapid in-situ growth of the AnAOB is applicable in the investigated regions. From the perspective of the removal performance of the TN and organic substance, the AnMBR-PN/A process has great potential as the layouts of the carbon-neutral mainstream wastewater treatment plants.

This study investigated the effect of combination of pretreatment methods such as ultra-sonication and electrolysis for the minimum energy input to recover the maximal carbohydrate and solubilization (in terms of sCOD) from mixed microalgae biomass. The composition of the soluble chemical oxygen demand (COD), protein, carbohydrate revealed that the hydrolysis method had showed positive impact on the increasing quantity and thus enhanced methane yields. As a result, the combination of these 2 pretreatments showed the greatest yield of soluble protein and carbohydrate as 279 and 309mg/L, which is the recovery of nearly 85 and 90% in terms of total content of them. BMP tests showed peak methane production yield of 257mL/gVSadded, for the hydrolysate of combined pretreatment as compared to the control experiment of 138mL/gVS added.

This research investigated the effects of various dilute acid (HCl, H 2 SO 4 , HNO 3 , H 3 PO 4 ) pretreatment on the solubilization of marine macroalgal biomass Gelidium amansii and subsequent hydrogen fermentation in a batch vials. The dry grounded biomass was hydrolyzed at temperature of 121 °C, solid/liquid (S/L) ratio of 5% (w/v), dilute acid concentration (1%) of various reagents, and reaction time of 30 min. The hydrolyzates obtained at these conditions were then fed to batch hydrogen fermentation. Results revealed that only H 2 SO 4 pretreatment method had a significant effect on improvement of biohydrogen production from Gelidium amansii , whereas the other pretreatment conditions were even worse than the control experiment. Dilute sulfuric acid (1%) provided a maximum hydrogen production of 52 mL-H 2 /g-dry biomass, whereas control experiment provided a hydrogen efficiency of 27 mL-H 2 /g-dry biomass. The results showed that selection of appropriate pretreatment method is essential for enhanced hydrogen production from macroalgal biomass.

Anaerobic digestion of decabromodiphenyl ether was carried out and compared in two continuously stirred anaerobic bioreactors for 210 days under thermophilic and mesophilic conditions. Results show that the degradation of decabromodiphenyl ether followed the first-order reaction kinetics, which exhibited a higher removal rate in the thermophilic reactor when compared to the mesophilic one, reaching its maximum of 1.1 μg·day−1. The anaerobic digestion of decabromodiphenyl ether was found to involve the replacement of bromines from polybrominated diphenyl ether by hydrogen atoms, gradually forming nona-, octa- and hepta-brominated diphenyl ether, respectively. Under the thermophilic condition, the reactors were dominated by Bacillus sp. and Methanosarcina sp. with high bioactivity and high concentrations of debromination microorganisms.

The application of biofilm-based processes such as up-flow anaerobic sludge blanket (UASB) reactors for saline wastewater treatment faces the problems of low strength and stability of granules cause by high salt concentration. In this study, the effect of biochar/Fe addition on biofilm formation was examined in anaerobic treatment of high salinity (10 g-Na+/L) wastewater with two parallel lab-scale UASBs. Higher removal efficiencies of soluble influent organics and methane production were achieved in the biochar/Fe seeded UASB reactor with an organic loading rate (OLR) ranging from 2.5 to 10 g-COD/L/d. The average SS concentration of the granular sludge in the biochar/Fe seeded UASB reactor reached 21.6 g/L while that in the non-biochar/Fe-seeded UASB one was 16.4 g/L. A higher extracellular polymeric substances (EPS) of 91.1 mg/g-VSS and medium particle size (D50) of 63.2 µm in the granular sludge with biochar/Fe addition were observed. All these results suggest that biochar/Fe addition can effectively improve biofilm formation under high salinity conditions.

This study investigated the potential and possibility of three mixed microalgae consortia collected from various ecological niches towards bioenergy production of H 2 and CH 4 in batch experiments under mesophilic conditions. Three different consortia collected from wastewater treatment plant, in an open pond system and in the lake bed possess different compositions and characteristics during their growth under a light intensity of 8000 lux and are referred as OP1, OP2 and LB. After 40 days of cultivation, collected wet biomass is directly used for H 2 and CH 4 fermentation and the results indicated that, consortia OP1 is good for H 2 production whereas consortia OP2 and LB showed nearly the similar CH 4 production performances. Peak hydrogen production rate (HPR) and methane production rate (MPR) were achieved as 289 mL/L-d and 97 mL/L-d, respectively from consortia OP1 and OP2. The energy production from this process could significantly contribute towards CO 2 emission reduction. Besides, this approach could be helpful in choosing the consortia towards which kind of biofuel (either H 2 or CH 4 ) production.

Biohydrogen production from lignocellulosic biomass (LCB) is an active research area. Several workers have tested a number of substrates under different operational conditions and brought forward the many positive process performance features and identified the main sources of inhibition. This review analyzes selected fermentative biohydrogen production processes by revisiting the core biohydrogen production performances in terms of gas production rates and yields and equally addresses the options for process enhancement by the application of through pretreatment methods and detoxification of process inhibitors. In addition, the issues related to continuous biohydrogen operation in different reactor configurations are highlighted. Lastly, future avenues of research which may be engendered and engineered to enhance the biohydrogen generation and process biokinetics are discussed. This review intends to provide the fundamental understanding of biohydrogen production and provides a perspective on future developments in this area of applied research.

The dispersion of granules in upflow anaerobic sludge blanket (UASB) reactor represents a critical technical issue in methanolic wastewater treatment. In this study, the potentials of coupling a microbial electrolysis cell (MEC) into an UASB reactor for improving methanolic wastewater biodegradation, long-term process stability and biomethane recovery were evaluated. The results indicated that coupling a MEC system was capable of improving the overall performance of UASB reactor for methanolic wastewater treatment. The combined system maintained the comparatively higher methane yield and COD removal efficiency over the single UASB process through the entire process, with the methane production at the steady-state conditions approaching 1504.7 ± 92.2 mL-CH4 L−1-reactor d−1, around 10.1% higher than the control UASB (i.e. 1366.4 ± 71.0 mL-CH4 L−1-reactor d−1). The further characterizations verified that the input of external power source could stimulate the metabolic activity of microbes and reinforced the EPS secretion. The produced EPS interacted with Fe2+/3+ liberated during anodic corrosion of iron electrode to create a gel-like three-dimensional [-Fe-EPS-]n matrix, which promoted cell-cell cohesion and maintained the structural integrity of granules. Further observations via SEM and FISH analysis demonstrated that the use of bioelectrochemical stimulation promoted the growth and proliferation of microorganisms, which diversified the degradation routes of methanol, convert the wasted CO2 into methane and accordingly increased the process stability and methane productivity.

Restaurant grease trap waste (GTW) is a potentially feasible energy source that can be used as an alternative to liquid fossil fuels and natural gas. However, GTW must be collected from many different resources for energy production because restaurants in Japan only produce an average of 486 kg GTW per year. This study investigated the variation in oil properties and methane generation potential of 44 GTW samples from 13 types of restaurants during different seasons. The GTW samples had high variation in oil content and oil properties. Biofuel oil was extracted from most GTW samples at a high efficiency (more than 80 wt%). In contrast, the methane yield varied little among de-oiled GTW samples. All types of GTWs are potential sources of biomethane generation. GTW samples are classified into five major oil property clusters according to solid chemical stability. Oil properties depend little on the season and heavily on the restaurant type.