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© 2020 Elsevier B.V. Climatic conditions and vegetation constrain the use of optical satellite imagery as an exploration tool for hydrothermal ore mineralization in tropical and subtropical regions. In this investigation, Landsat-8 and ASTER satellite imagery were used to detect hydrothermal alteration zones associated with gold mineralization in the Ngoura-Colomines region, Eastern Cameroon. The study area contains several gold-bearing quartz veins associated with zones of pyritization, muscovite/sericite, iron oxides, and silicification. Principal Component Analysis (PCA), Independent Component Analysis (ICA), and specialized spectral band ratios were used to extract spectral information related to vegetation, iron oxide/hydroxide minerals, Al–OH, Fe-Mg–OH, carbonate group minerals, and silicification using Landsat-8 data at regional scale. Linear Spectral Unmixing (LSU) algorithm was implemented to ASTER VNIR + SWIR bands for detailed discrimination of hematite, jarosite, kaolinite, muscovite, chlorite and epidote at district scale. The Automated Spectral Hourglass (ASH) technique was employed to extract reference spectra directly from the ASTER bands for producing fraction images of end-members using the LSU. A comprehensive field survey was used to verify the remote sensing results. Petrographic study, X-ray diffraction analysis and reflectance spectroscopy indicated the presence of quartz, goethite and sericite, as well as the absorption features of Fe3+/Fe2+, Al–OH, OH/H2O and SiO2 in the alteration zones. Several hydrothermal alteration zones of iron oxide/hydroxide, clay, carbonate minerals and silicification zones were identified, which are spatially associated with known mining areas and gold occurrences in the study area. High potential prospects were also delineated, including the Ngoura-Colomines prospects and the newly discovered Yangamo-Ndatanga and Taparé-Tapondo prospects in the southwestern and southeastern parts of the study area. Consequently, satellite-based mineral prospectivity maps at regional and district scales were generated for the study area by implementing the fuzzy logic model to the most informative thematic layers derived from image processing results. The satellite-based prospectivity maps are reliable for exploration of new gold prospective zones in the Ngoura-Colomines goldfield.