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Your search keyword '"Takiguchi, Yoshiharu"' showing total 239 results
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239 results on '"Takiguchi, Yoshiharu"'

2. Reversal of neuroinflammation in novel GS model mice by single i.c.v. administration of CHO-derived rhCTSA precursor protein

Author

Horii, Yuto, primary, Iniwa, Toshiki, additional, Onitsuka, Masayoshi, additional, Tsukimoto, Jun, additional, Tanaka, Yuki, additional, Ike, Hironobu, additional, Fukushi, Yuri, additional, Ando, Haruna, additional, Takeuchi, Yoshie, additional, Nishioka, So-ichiro, additional, Tsuji, Daisuke, additional, Ikuo, Mariko, additional, Yamazaki, Naoshi, additional, Takiguchi, Yoshiharu, additional, Ishimaru, Naozumi, additional, and Itoh, Kohji, additional

Published
2022
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8. Nitrite is an alternative source of NO in vivo

Author

Tsuchiya, Koichiro, Kanematsu, Yasuhisa, Yoshizumi, Masanori, Ohnishi, Hideki, Kirima, Kazuyoshi, Izawa, Yuki, Shikishima, Michiyo, Ishida, Tatsuhiro, Kondo, Shuji, Kagami, Shoji, Takiguchi, Yoshiharu, and Tamaki, Toshiaki

Subjects
Hypertension -- Research, Nitric oxide -- Research, Biological sciences
Abstract

In this study, we investigated whether orally administered nitrite is changed to NO and whether nitrite attenuates hypertension in a dose-dependent manner. We utilized a stable isotope of [[sup.15]N]nitrite ([sup.15]N[O.sup.-.sub.2]) as a source of nitrite to distinguish between endogenous nitrite and that exogenously administered and measured hemoglobin (Hb)-NO as an index of circulating NO in whole blood using electron paramagnetic resonance (EPR) spectroscopy. When 1 mg/kg [Na.sup.15]N[O.sub.2] was orally administered to rats, an apparent EPR signal derived from [Hb.sup.15]NO ([A.sub.z] = 23.4 gauss) appeared in the blood. The peak blood HbNO concentration occurred at the first measurement after intake (5 min) for treatment with 1 and 3 mg/kg (HbNO: 4.93 [+ or -] 0.52 and 10.58 [+ or -] 0.40 [micro]M, respectively) and at 15 min with 10 mg/kg (HbNO: 38.27 [+ or -] 9.23 [micro]M). In addition, coadministration of nitrite (100 mg/l drinking water) with [N.sup.[omega]]-nitro-L-arginine methyl ester (L-NAME; 1 g/l) for 3 wk significantly attenuated the L-NAME-induced hypertension (149 [+ or -] 10 mmHg) compared with L-NAME alone (170 [+ or -] 13 mmHg). Furthermore, this phenomenon was associated with an increase in circulating HbNO. Our findings clearly indicate that orally ingested nitrite can be an alternative to L-arginine as a source of NO in vivo and may explain, at least in part, the mechanism of the nitrite/nitrate-rich Dietary Approaches to Stop Hypertension diet-induced hypotensive effects. nitric oxide; hypertension; electron paramagnetic resonance

Published
2005

17. Use of modified U1 small nuclear RNA for rescue from exon 7 skipping caused by 5′-splice site mutation of human cathepsin A gene

Author

Yamazaki, Naoshi, Kanazawa, Keisuke, Kimura, Maria, Ike, Hironobu, Shinomiya, Makiko, Tanaka, Shouko, Shinohara, Yasuo, Minakawa, Noriaki, Itoh, Kohji, Takiguchi, Yoshiharu, Yamazaki, Naoshi, Kanazawa, Keisuke, Kimura, Maria, Ike, Hironobu, Shinomiya, Makiko, Tanaka, Shouko, Shinohara, Yasuo, Minakawa, Noriaki, Itoh, Kohji, and Takiguchi, Yoshiharu

Abstract

Cathepsin A (CTSA) is a multifunctional lysosomal enzyme, and its hereditary defect causes an autosomal recessive disorder called galactosialidosis. In a certain number of galactosialidosis patients, a base substitution from adenine to guanine is observed at the +3 position of the 7th intron (IVS7 +3a>g) of the CTSA gene. With this mutation, a splicing error occurs; and consequently mRNA lacking the 7th exon is produced. This skipping of exon 7 causes a frame shift of the transcripts, resulting in a non-functional CTSA protein and hence galactosialidosis. This mutation seems to make the interaction between the 5’-splice site of intron 7 of pre-mRNA and U1 small nuclear RNA (U1 snRNA) much weaker. In the present study, to produce properly spliced mRNA from the CTSA gene harboring this IVS7 +3a>g mutation, we examined the possible usefulness of modified U1 snRNA that could interact with the mutated 5’-splice site. Toward this goal, we first prepared a model system using a mutant CTSA mini gene plasmid for delivery into HeLa cells. Then, we examined the effectiveness of modified U1 snRNA on the formation of properly spliced mRNA from this mutant CTSA mini gene. As a result, we succeeded in obtaining improved formation of properly spliced CTSA mRNA. Our results suggest the usefulness of modified U1 snRNA for rescue from exon 7 skipping caused by the IVS7 +3a>g mutation of the CTSA gene.

Published
2018

20. Synergistic effect of combining theophylline and drugs that potentially elevate serum creatine kinase

Author

Iwano, Junko, Nakamura, Yoichi, Izumida, Takashi, Yokota, Masayuki, Takiguchi, Yoshiharu, Minakuchi, Kazuo, and Sone, Saburo

Subjects
drug interaction, creatine kinase, theophylline
Abstract

An increase in the serum creatine kinase (CK) level is one of the side effects of theophylline;on rare occasions, the increase may be followed by rhabdomyolysis. Theophylline is often administered with drugs that potentially elevate the serum CK level (CK-elevating drugs) such as β-agonists and steroids. However, the effects of the combined treatment of theophylline and CK-elevating drugs have not been reported. We, therefore, retrospectively investigated the effects of combined treatment on the serum CK level, in391asthmatic outpatients. In this study, the number and type of the CK-elevating drugs administered, and the serum levels of CK and theophylline, were investigated. The patients were divided into four groups:the theophylline-treated and CK-elevating drug-treated group, the theophylline-treated and non-CK-elevating drug-treated group, the non-theophylline-treated and CK-elevating drug-treated group, and the non-theophylline-treated and non-CK-elevating drug-treated group. The theophylline-treated and CK-elevating drug-treated group showed about 100%higher serum CK levels (225IU/L) than any other group (102-124IU/L), and no increase in the serum theophylline level. This result indicates that there is a synergistic effect of theophylline and CK-elevating drugs on the serum CK level. The combined treatment of theophylline and CK-elevating drugs induces a synergistic increase in the serum CK level, indicating not pharmacokinetic but pharmacodynamic interactions with these drugs.

Published
2000

23. Prevention of lethal hepatic injury in Long-Evans Cinnamon (LEC) rats by D-galactosamine hydrochloride

Author

Otsuka, Toshihiro, Izumi, Keisuke, Tokunaga, Itsuo, Gotohda, Takako, Ipposhi, Kaneshige, Takiguchi, Yoshiharu, Kaneda, Shinya, Satake, Nobuo, Ohnishi, Takamasa, Tashiro, Seiki, Shimada, Mitsuo, Otsuka, Toshihiro, Izumi, Keisuke, Tokunaga, Itsuo, Gotohda, Takako, Ipposhi, Kaneshige, Takiguchi, Yoshiharu, Kaneda, Shinya, Satake, Nobuo, Ohnishi, Takamasa, Tashiro, Seiki, and Shimada, Mitsuo

Abstract

Repeated injections of D-galactosamine hydrochloride (GalN) increase the survival rate of Long-Evans Cinnamon (LEC) rats, an animal model of Wilson’s disease. The aim of the present study was to investigate the mechanism of GalN for prevention of spontaneous lethal hepatic injury in LEC rats. MaleLEC rats were given a single subcutaneous injection of 300mg/kg of GalN or vehicle (0.9%NaCl) at 14weeks, and killed at 28 weeks of age. Next, 6-week-old male LEC rats were given weekly subcutaneous injections of 300 mg/kg of GalN or vehicle for 3 or 12 weeks, and their hepatic 8-hydroxydeoxy-2’-guanosine (8-OHdG), glutathione peroxidase (GPX), and catalase activities were measured. None of GalN-treated rats died of hepatic injury (0/12), whereas the mortality rate of control rats given 0.9% NaCl was 17% (2/12). GalN administration for 12 weeks decreased the hepatic 8-OHdG, and GalN administration for either 3 or 12weeks increased the glutathione peroxidase activity. GalN administration increased the serum level of alanine aminotransferase, and accelerated megalocytic degeneration of the hepatocytes. GalN treatment is effective in preventing lethal hepatitis in LEC rats and decrease of oxidative DNA damage by GalN plays an important role in increase of the survival rate.

Published
2006

28. Infection Treatment Caused by Multiple-drug-resistant Pseudomonas aeruginosa in a Patient Underwent Allogeneic Hematopoietic Stem Cell Transplantation

Author

KAWAZOE, Hitoshi, primary, TAKIGUCHI, Yoshiharu, additional, INOUE, Tatsuya, additional, YAMAGUCHI, Kazunori, additional, TANAKA, Hiroaki, additional, KAJI, Masato, additional, TSUJI, Shigeko, additional, NINOMIYA, Masaki, additional, FUKUOKA, Noriyasu, additional, OHNISHI, Hiroaki, additional, ISHIDA, Toshihiko, additional, and HOUCHI, Hitoshi, additional

Published
2008
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33. Monitoring the Side Effects of Cancer Chemotherapy with Patients-The Participation of Patients in Cancer Therapy and Sharing Patient Information-

Author

KAWAZOE, Hitoshi, primary, KUBO, Tomomi, additional, IIHARA, Naomi, additional, DOI, Chiaki, additional, OKUJYOH, Makiko, additional, FUKUOKA, Noriyasu, additional, FUJIMOTO, Satoshi, additional, KANAJI, Nobuhiro, additional, BANDOH, Shuji, additional, ISHIDA, Toshihiko, additional, TAKIGUCHI, Yoshiharu, additional, and HOUCHI, Hitoshi, additional

Published
2006
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35. Folia Pharmacologica Japonica

Author

Tajima, Soichiro, primary, Tsuchiya, Koichiro, additional, Ohnishi, Hideki, additional, Kanematsu, Yasuhisa, additional, Tamaki, Toshiaki, additional, Mason, Ronald, additional, and Takiguchi, Yoshiharu, additional

Published
2005
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43. Pharmacokinetics and Safety of a New Parenteral Carbapenem Antibiotic, Biapenem (L-627), in Elderly Subjects

Author

Kozawa, Osamu, primary, Uematsu, Toshihiko, additional, Matsuno, Hiroyuki, additional, Niwa, Masayuki, additional, Takiguchi, Yoshiharu, additional, Matsumoto, Syouzou, additional, Minamoto, Masahiko, additional, Niida, Yoshito, additional, Yokokawa, Masahiro, additional, Nagashima, Satoru, additional, and Kanamaru, Mitsutaka, additional

Published
1998
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