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747 results on '"Takano, Yasuo"'

16. Establishment of patient-derived cancer xenografts in immunodeficient NOG mice

Author

CHIJIWA, TSUYOSHI, KAWAI, KENJI, NOGUCHI, AKIRA, SATO, HIDEMITSU, HAYASHI, AKIMUNE, CHO, HARUHIKO, SHIOZAWA, MANABU, KISHIDA, TAKESHI, MORINAGA, SOICHIRO, YOKOSE, TOMOYUKI, KATAYAMA, MAKOTO, TAKENAKA, NOBUO, SUEMIZU, HIROSHI, YAMADA, ROPPEI, NAKAMURA, YOSHIYASU, OHTSU, TAKASHI, TAKANO, YASUO, IMAI, KOHZOH, MIYAGI, YOHEI, and NAKAMURA, MASATO

Published
2015
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22. Increased death receptor pathway of apoptotic signaling in septic mouse aorta: effect of systemic delivery of FADD siRNA

Author

Matsuda, Naoyuki, Teramae, Hiroki, Yamamoto, Seiji, Takano, Ken-Ichi, Takano, Yasuo, and Hattori, Yuichi

Subjects
Apoptosis -- Genetic aspects, Apoptosis -- Research, Cellular signal transduction -- Physiological aspects, Cellular signal transduction -- Genetic aspects, Cellular signal transduction -- Research, RNA -- Physiological aspects, Biological sciences
Abstract

Am J Physiol Heart Circ Physiol 298: H92-H101, 2010. First published October 23, 2009: doi: 10.1152/ajpheart.00069.2009.--Recent evidence suggests that apoptotic cell death plays an important role in the pathophysiology of sepsis. Because there is extensive apoptosis of vascular endothelial cells in sepsis, we examined whether the death receptor pathway of apoptotic signaling is altered in thoracic aortas from mice with polymicrobial sepsis, as produced by cecal ligation and puncture (CLP). In septic aorta, total and surface expression levels of the two death receptors tumor necrosis factor receptor 1 and Fas were highly upregulated. Furthermore, marked increases in the mRNA and protein levels of Fas-associated death domain (FADD), an adaptor molecule to recruit procaspase-8 into the death-inducing signal complex, were observed in septic aorta, which were strongly suppressed by systemic delivery of small interfering RNA (siRNA) against FADD. No increase in expression of death receptors and FADD was observed in endothelium-denuded aortic tissues from septic animals. Systemic administration of FADD siRNA also resulted in great attenuation of sepsis-induced increases in expression and activation of caspase-3, an effector protease in the apoptosis cascade. Terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) revealed that the significant appearance of cell apoptosis in aortic endothelium after CLP-induced sepsis was eliminated when FADD siRNA was systemically applied. Light and electron microscopic examinations of septic aorta showed cell swelling, nuclear fragmentation, and partial detachment of endothelial cells from the basal membrane, which were prevented by systemic treatment with FADD siRNA. Finally, FADD siRNA administration dramatically improved survival of CLP mice, supporting the feasibility of this gene-based approach for treating septic shock. sepsis; apoptosis; endothelium

Published
2010

33. Modulation of glucocorticoid receptor expression, inflammation, and cell apoptosis in septic guinea pig lungs using methylprednisolone

Author

Kamiyama, Koki, Matsuda, Naoyuki, Yamamoto, Seiji, Takano, Ken-ichi, Takano, Yasuo, Yamazaki, Hiromi, Kageyama, Shun-ichiro, Yokoo, Hiroki, Nagata, Takuya, Hatakeyama, Noboru, Tsukada, Kazuhiro, and Hattori, Yuichi

Subjects
Apoptosis -- Health aspects, Apoptosis -- Genetic aspects, Apoptosis -- Research, Hormone receptors -- Health aspects, Hormone receptors -- Genetic aspects, Hormone receptors -- Research, Methylprednisolone -- Dosage and administration, Septic shock -- Care and treatment, Septic shock -- Research, Biological sciences
Abstract

The use of glucocorticoids for treatment of sepsis has waxed and waned during the past several decades, and recent randomized controlled trials have evoked a reassessment of this therapy. Most glucocorticoid actions are mediated by its specific intracellular receptors (GRs). Thus we initially evaluated whether sepsis and high-dose corticosteroid therapy can regulate guinea pig pulmonary expression of GRs: active receptor, GR[alpha], and dominant negative receptor, GR[beta]. Sepsis induction by LPS injection (300 [micro]g/kg ip) decreased mRNA and protein levels of GR[alpha] and increased protein expression of GR[beta] in lungs. High-dose methylprednisolone (40 mg/kg ip), administered simultaneously with LPS, markedly potentiated the decrease in GR[alpha] expression but slightly affected the increase in GR[beta] expression. Consequently, this led to a significant reduction in GR[alpha] nuclear translocation. Nevertheless, methylprednisolone treatment strongly eliminated LPS induction of NF-[kappa]B activity, as determined by NF-[kappa]B nuclear translocation and by gel mobility shift assays. Furthermore, the LPS-induced increase in inflammatory cells in bronchoalveolar lavage fluid was blunted by administration of the corticosteroid. On the other hand, immunofluorescent staining for cleaved caspase-3 showed a marked increase in this proapoptotic marker in lung sections, and terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) represented an enhanced appearance of cell apoptosis in lungs and spleen when methylprednisolone was given together with LPS. Cell apoptosis is now considered to play a role in the pathogenesis of septic syndrome. We thus suggest that the action of glucocorticoids at high doses to accelerate sepsis-induced cell apoptosis may overwhelm their therapeutic advantages in septic shock. lung inflammation; nuclear factor-[kappa]B

Published
2008

38. Jamestown Canyon virus detection in human tissue specimens

Author

Zheng, Huachuan, Murai, Yoshihiro, Hong, Mei, Nakanishi, Yuko, Nomoto, Kazuhiro, Masuda, Shinji, Tsuneyama, Koichi, and Takano, Yasuo

Subjects
Arbovirus diseases -- Diagnosis, Arbovirus diseases -- Research, Tissue microarrays -- Research, Tissue microarrays -- Usage, Health
Published
2007

40. Expression of KAI1 and tenascin, and microvessel density are closely correlated with liver metastasis of gastrointestinal adenocarcinoma

Author

Zheng, Huachuan, Tsuneyama, Koichi, Cheng, Chunmei, Takahashi, Hiroyuki, Cui, Zhengguo, Nomoto, Kazuhiro, Murai, Yoshihiro, and Takano, Yasuo

Subjects
Adenocarcinoma -- Diagnosis, Adenocarcinoma -- Research, Gastrointestinal cancer -- Diagnosis, Gastrointestinal cancer -- Research, Gastrointestinal cancer -- Development and progression, Genetic markers -- Usage, Liver diseases -- Development and progression, Liver diseases -- Diagnosis, Liver diseases -- Genetic aspects, Gene expression -- Research, Health
Published
2007

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