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Fructose is considered to negatively affect type 2 diabetes mellitus (T2DM); however, there are contradictory reports. The present study aimed to elucidate the effects of fructose-rich diet (FRD) on glucose metabolism of Wistar Bonn Kobori (WBN/Kob) fatty diabetic (WBKDF) rats, a spontaneous T2DM model, and Wistar rats. Wistar Bonn Kobori fatty diabetic and Wistar rats were fed either FRD or standard diet (STD) for 4 weeks. The food intake, body weight, plasma glucose and insulin were measured weekly. After the 4-week challenge, rats were subjected to an intravenous glucose tolerance test (IVGTT). The liver and pancreas were used for histological analysis. The 4-week challenge of FRD in Wistar rats did not cause hyperglycaemia, but increased insulin resistance (Homeostatic Model Assessment for Insulin Resistance [HOMA-IR]). Feeding WBKDF rats with a FRD accelerated obesity, but prevented the onset of severe hyperglycaemia via maintaining high plasma insulin levels. Homeostatic Model Assessment for Insulin Resistance in WBKDF rats was not changed by FRD feeding. Intravenous glucose tolerance test revealed that FRD feeding in Wistar rats did not affect glucose tolerance, but slightly increased the plasma insulin level. In contrast, FRD feeding in WBKDF rats significantly reduced the glucose tolerance, but insulin response was not improved. Fructose-rich diet feeding did not alter the β cell area in Wistar rats, but significantly increased it in WBKDF rats. In conclusion, FRD caused insulin resistance in Wistar rats, suggesting that fructose overconsumption is a risk factor for T2DM, whereas FRD inhibited severe hyperglycaemia by maintaining high insulin levels in WBKDF rats. Fructose may be a beneficial sugar for T2DM patients with severe obesity-induced insulin resistance.

How do separate sexes originate and evolve? Plants provide many opportunities to address this question as they have diverse mating systems and separate sexes (dioecy) that evolved many times independently. The classic “two-factor” model for evolution of separate sexes proposes that males and females can evolve from hermaphrodites via the spread of male and female sterility mutations that turn hermaphrodites into females and males, respectively. This widely accepted model was inspired by early genetic work in dioecious white campion (Silene latifolia) that revealed the presence of two sex-determining factors on the Y-chromosome, though the actual genes remained unknown. Here, we report identification and functional analysis of the putative sex-determining gene in S. latifolia, corresponding to the gynoecium suppression factor (GSF). We demonstrate that GSF likely corresponds to a Y-linked CLV3-like gene that is specifically expressed in early male flower buds and encodes the protein that suppresses gynoecium development in S. latifolia. Interestingly, GSFY has a dysfunctional X-linked homolog (GSFX) and their synonymous divergence (dS = 17.9%) is consistent with the age of sex chromosomes in this species. We propose that female development in S. latifolia is controlled via the WUSCHEL-CLAVATA feedback loop, with the X-linked WUSCHEL-like and Y-linked CLV3-like genes, respectively. Evolution of dioecy in the S. latifolia ancestor likely involved inclusion of ancestral GSFY into the nonrecombining region on the nascent Y-chromosome and GSFX loss of function, which resulted in disbalance of the WUSCHEL-CLAVATA feedback loop between the sexes and ensured gynoecium suppression in males.

The importance of glucose is well known as an energy source in testes. In order to evaluate the effects of long-lasting hypoglycemia on testes, a novel glucokinase activator, TMG-123, was dosed to rats at 5, 20 and 100 mg/kg for 13 weeks. As a result, plasma glucose levels decreased for several hours with increasing doses over the dose range of 5 to 100 mg/kg. No toxicological findings attributable to the test article were observed in clinical observation, measurements of body weight and food consumption, necropsy, and organ weight measurement. Histopathology showed scattered degeneration of seminiferous tubules in testes, and exfoliation of germ cells related to the degeneration of seminiferous tubules was observed in the lumen of both epididymides in the same animals at the end of the dosing period. Similar histopathological findings were noted at the end of the recovery period. In addition, a fertility study was conducted at the same doses for 13 weeks for males and 5 weeks for females. Sperm analysis showed decreases in the sperm concentration and the motility index and an increase in the incidences of sperm malformations. However, there were no abnormalities in the copulation or fertility rate. These results suggest that long-lasting hypoglycemia in rats is harmful to spermatogenesis and the testicular damage does not recover.

Heavy ion beam probe (HIBP) systems have been designed for the new tokamak, PLATO [A. Fujisawa, AIP Conf. Proc. 1993, 020011 (2018)]. The designs have been completed, and the installations are in progress. Two HIBPs are being installed in toroidal sections 180° apart to investigate long-range correlations in the toroidal direction. Each HIBP consists of an injection beamline and a detection beamline as usual. Yet, one of the HIBPs is equipped with an additional detection beamline; the measurement positions of its two detection beamlines can be placed on almost the same magnetic surface yet at poloidal angles that differ by ∼180°. The use of three detection beamlines allows us to investigate spatial asymmetry and long-range correlations in both the toroidal and poloidal directions, simultaneously. The detected beam intensity is expected to be enough for turbulence measurements in almost the entire plasma region when the electron density is up to 1 × 1019 m−3 by selecting appropriate ion species for the probe beam. Each detector has three channels 10 mm apart, allowing measurement of local structures of micro-scale turbulence. Therefore, using the HIBPs on the PLATO tokamak will enable both local and global properties of plasma turbulence to be investigated, simultaneously.

The conditional average is a technique to extract a typical waveform from bursty or quasi-periodic phenomena regarded as a combination of deterministic trend and residual fluctuations (or probabilistic part). The article proposes a new conditional average technique that is an extension from what is called the template method that allows automatic selection, using the correlation analysis, of the identical events of the phenomena. The proposed method is applied on a quasi-periodic oscillation observed in a linear magnetized plasma, and successfully divides the phenomenon into the deterministic trend and residual fluctuations. Moreover, the statistical error analysis on the power of the residual fluctuations discloses the presence of mutual interactions between the deterministic trend and the residual fluctuations.

It has been believed that isoamylase (ISA)-type α-glucan debranching enzymes (DBEs) play crucial roles not only in α-glucan degradation but also in the biosynthesis by affecting the structure of glucans, although molecular basis on distinct roles of the individual DBEs has not fully understood. In an attempt to relate the roles of DBEs to their chain-length specificities, we analyzed the chain-length distribution of DBE enzymatic reaction products by using purified DBEs from various sources including rice, cyanobacteria, and bacteria. When DBEs were incubated with phytoglycogen, their chain-length specificities were divided into three groups. First, rice endosperm ISA3 (OsISA3) and Eschericia coli GlgX (EcoGlgX) almost exclusively debranched chains having degree of polymerization (DP) of 3 and 4. Second, OsISA1, Pseudomonas amyloderamosa ISA (PsaISA), and rice pullulanase (OsPUL) could debranch a wide range of chains of DP≧3. Third, both cyanobacteria ISAs, Cyanothece ATCC 51142 ISA (CytISA) and Synechococcus elongatus PCC7942 ISA (ScoISA), showed the intermediate chain-length preference, because they removed chains of mainly DP3-4 and DP3-6, respectively, while they could also react to chains of DP5-10 and 7–13 to some extent, respectively. In contrast, all these ISAs were reactive to various chains when incubated with amylopectin. In addition to a great variation in chain-length preferences among various ISAs, their activities greatly differed depending on a variety of glucans. Most strikingly, cyannobacteria ISAs could attack branch points of pullulan to a lesser extent although no such activity was found in OsISA1, OsISA3, EcoGlgX, and PsaISA. Thus, the present study shows the high possibility that varied chain-length specificities of ISA-type DBEs among sources and isozymes are responsible for their distinct functions in glucan metabolism. 11;6

OBJECT: Biopsy is generally necessary for the diagnosis of primary central nervous system lymphoma PCNSL. However, surgical biopsy has problems of fatal hemorrhagic complication and false negative findings, so more safe and reliable diagnostic methods are required. The aim of this present study is to detect MYD88 mutations, an important driver mutation, in cerebrospinal fluid (CSF) taken from PCNSL patients. METHOD: Fifteen CNS lymphoma (13 PCNSL, 3 CNS relapse from systemic lymphoma) patients were studied. We obtained cell free DNA (cfDNA) from CSF by lumbar puncture. CfDNA was extracted from 1ml of CSF, and both direct sequence and droplet digital PCR (ddPCR) were performed. Furthermore, we performed direct sequence from surgical obtained formalin fixed paraffin embedded (FFPE) tissue and analyzed the relationship to clinical data. RESULT: The mean cfDNA concentration of 1 ml CSF was 188.8 ng/ml [95% CI 124.2–253.8 ng/ml]. MYD88 mutations from CSF were detected in 60.0% (9 of 15 cases), and L265P in exon5 was the most frequent mutation in 8 out of 9 (88.8%) cases, S219C in exon3 was detected in one case. In 2 patients, MYD88 mutation was confirmed by ddPCR but not direct sequence. In all 8 cases with sufficient FFPE tissue for DNA analysis, MYD88 mutation was confirmed in the FFPE tissue. In 1 patient, there was insufficient FFPE tissue for DNA analysis. MYD88 mutation was not detected in the CSF of all 6 patients without MYD88 mutation in the tumor. CONCLUSION: This pilot study provided evidence that the somatic driver mutation MYD88 can be reliably detected by direct sequence and ddPCR in the cfDNA taken from 1 ml of CSF in patients with CNS lymphomas.

The purpose of this study is to develop a procedure for carrying out analysis on torsional vibration of a mechanical system including piston-crank systems such as a reciprocating compressor. In the present study, we adopted the mechanical system consisting of a motor, a fly wheel and multiple piston-crank systems. We have derived equations of motion considering both variable moment of inertia of a piston-crank system through revolution of the crank and the boundary conditions that both ends of the crankshaft are free. The computer program for eigenvalue analysis and the one for time history responses have been developed using the derived equations of motion. The dynamic characteristics of a model system were investigated comparing the calculated results of eigenvalue analysis with the experimental results. The time history responses on torsion of the crankshafts of the model system were also calculated.

This paper describes a change in optical spectrum on propagation in free space for a particular class of spatially partially coherent fields emanating from a polychromatic secondary source. A rectangular opening aperture in an opaque screen, illuminated with an extended incoherent polychromatic primary source, works as the secondary source. The spectral change is explored in detail theoretically and experimentally in association with spatial coherence in the secondary source as well as dispersive diffraction by the secondary source. The peak shift of the spectrum is associated with the coherence area for characterizing the secondary source. The peak shift becomes maximum if the secondary source is spatially coherent, but no shift occurs if the secondary source is spatially incoherent.

RhoA, a small GTPase, is one of the key proteins of smooth muscle contraction. In allergic asthma, an upregulation of RhoA in bronchial smooth muscle has been suggested. However, the mechanism of its upregulation has not yet been clarified. In the present study, the effects of interleukin-4 (IL-4), one of the T-helper 2 cytokines, on RhoA mRNA expression and promoter activity of RhoA gene were examined in cultured human bronchial smooth muscle cells (hBSMCs). The quantitative real-time RT-PCR analyses revealed that incubation of hBSMCs with IL-4 (10, 30 and 100 ng/mL, for 24 hr) caused an increase in RhoA mRNA in a concentration-dependent manner. In luciferase reporter gene assay using hBSMCs that were transfected with luciferase constructs and were then stimulated with IL-4 (100 ng/mL), an importance of the most proximal STAT6 binding region (78-70 bp upstream of the transcription initiation site) was suggested. It is thus possible that IL-4 is capable of upregulating RhoA by promoting its transcription in hBSMCs. The proximal STAT6 binding region is required for the IL-4-induced increase in promoter activity of the human RhoA gene.

定性分析において第IV属陽イオンは,アンモニアアルカリ性で硫化物として沈殿させたのち,沈殿を希塩酸で処理すると,亜鉛,マンガンは溶解するが,コバルト,ニッケルは不溶物として残るので相互に分離される.種々の割合で(1)亜鉛-マンガン,(2)コバルト-マンガンを含む硫化物沈殿をつくり,ESRにより沈殿中のマンガンの分布状態を調べた.マンガンは(1)においても(2)においても,均一に分布していることがわかつた.