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1,162 results on '"Tahara, H"'

1. First cryogenic test operation of underground km-scale gravitational-wave observatory KAGRA

Author

KAGRA Collaboration, Akutsu, T., Ando, M., Arai, K., Arai, Y., Araki, S., Araya, A., Aritomi, N., Asada, H., Aso, Y., Atsuta, S., Awai, K., Bae, S., Baiotti, L., Barton, M. A., Cannon, K., Capocasa, E., Chen, C-S., Chiu, T-W., Cho, K., Chu, Y-K., Craig, K., Creus, W., Doi, K., Eda, K., Enomoto, Y., Flaminio, R., Fujii, Y., Fujimoto, M. -K., Fukunaga, M., Fukushima, M., Furuhata, T., Hagiwara, A., Haino, S., Hasegawa, K., Hashino, K., Hayama, K., Hirobayashi, S., Hirose, E., Hsieh, B. H., Huang, C-Z., Ikenoue, B., Inoue, Y., Ioka, K., Itoh, Y., Izumi, K., Kaji, T., Kajita, T., Kakizaki, M., Kamiizumi, M., Kanbara, S., Kanda, N., Kanemura, S., Kaneyama, M., Kang, G., Kasuya, J., Kataoka, Y., Kawai, N., Kawamura, S., Kawasaki, T., Kim, C., Kim, J., Kim, J. C., Kim, W. S., Kim, Y. -M., Kimura, N., Kinugawa, T., Kirii, S., Kitaoka, Y., Kitazawa, H., Kojima, Y., Kokeyama, K., Komori, K., Kong, A. K. H., Kotake, K., Kozu, R., Kumar, R., Kuo, H-S., Kuroyanagi, S., Lee, H. K., Lee, H. M., Lee, H. W., Leonardi, M., Lin, C-Y., Lin, F-L., Liu, G. C., Liu, Y., Majorana, E., Mano, S., Marchio, M., Matsui, T., Matsushima, F., Michimura, Y., Mio, N., Miyakawa, O., Miyamoto, A., Miyamoto, T., Miyo, K., Miyoki, S., Morii, W., Morisaki, S., Moriwaki, Y., Morozumi, T., Murakami, I., Musha, M., Nagano, K., Nagano, S., Nakamura, K., Nakamura, T., Nakano, H., Nakano, M., Nakao, K., Namai, Y., Narikawa, T., Naticchioni, L., Quynh, L. Nguyen, Ni, W. -T., Nishizawa, A., Obuchi, Y., Ochi, T., Oh, J. J., Oh, S. H., Ohashi, M., Ohishi, N., Ohkawa, M., Okutomi, K., Ono, K., Oohara, K., Ooi, C. P., Pan, S-S., Park, J., Arellano, F. E. Peña, Pinto, I., Sago, N., Saijo, M., Saito, Y., Saitou, S., Sakai, K., Sakai, Y., Sasai, M., Sasaki, M., Sasaki, Y., Sato, N., Sato, S., Sato, T., Sekiguchi, Y., Seto, N., Shibata, M., Shimoda, T., Shinkai, H., Shishido, T., Shoda, A., Somiya, K., Son, E. J., Suemasa, A., Suzuki, T., Tagoshi, H., Tahara, H., Takahashi, H., Takahashi, R., Takamori, A., Takeda, H., Tanaka, H., Tanaka, K., Tanaka, T., Tanioka, S., Martin, E. N. Tapia San, Tatsumi, D., Terashima, S., Tomaru, T., Tomura, T., Travasso, F., Tsubono, K., Tsuchida, S., Uchikata, N., Uchiyama, T., Ueda, A., Uehara, T., Ueki, S., Ueno, K., Uraguchi, F., Ushiba, T., van Putten, M. H. P. M., Vocca, H., Wada, S., Wakamatsu, T., Watanabe, Y., Xu, W-R., Yamada, T., Yamamoto, A., Yamamoto, K., Yamamoto, S., Yamamoto, T., Yokogawa, K., Yokoyama, J., Yokozawa, T., Yoon, T. H., Yoshioka, T., Yuzurihara, H., Zeidler, S., and Zhu, Z. -H.

Subjects
Astrophysics - Instrumentation and Methods for Astrophysics, General Relativity and Quantum Cosmology, Physics - Instrumentation and Detectors
Abstract

KAGRA is a second-generation interferometric gravitational-wave detector with 3-km arms constructed at Kamioka, Gifu in Japan. It is now in its final installation phase, which we call bKAGRA (baseline KAGRA), with scientific observations expected to begin in late 2019. One of the advantages of KAGRA is its underground location of at least 200 m below the ground surface, which brings small seismic motion at low frequencies and high stability of the detector. Another advantage is that it cools down the sapphire test mass mirrors to cryogenic temperatures to reduce thermal noise. In April-May 2018, we have operated a 3-km Michelson interferometer with a cryogenic test mass for 10 days, which was the first time that km-scale interferometer was operated at cryogenic temperatures. In this article, we report the results of this "bKAGRA Phase 1" operation. We have demonstrated the feasibility of 3-km interferometer alignment and control with cryogenic mirrors., Comment: 23 pages, 11 figures

Published
2019
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2. Vibration isolation system with a compact damping system for power recycling mirrors of KAGRA

Author

Akiyama, Y., Akutsu, T., Ando, M., Arai, K., Arai, Y., Araki, S., Araya, A., Aritomi, N., Asada, H., Aso, Y., Bae, S., Baiotti, L., Barton, M. A., Cannon, K., Capocasa, E., Chen, C-S., Chiu, T-W., Cho, K., Chu, Y-K., Craig, K., Dattilo, V., Doi, K., Enomoto, Y., Flaminio, R., Fujii, Y., Fujimoto, M. -K., Fukunaga, M., Fukushima, M., Furuhata, T., Haino, S., Hasegawa, K., Hashimoto, Y., Hashino, K., Hayama, K., Hirayama, T., Hirose, E., Hsieh, B. H., Huang, C-Z., Ikenoue, B., Inoue, Y., Ioka, K., Itoh, Y., Izumi, K., Kaji, T., Kajita, T., Kakizaki, M., Kamiizumi, M., Kanbara, S., Kanda, N., Kanemura, S., Kang, G., Kasuya, J., Kawai, N., Kawasaki, T., Kim, C., Kim, W. S., Kim, J., Kim, J. C., Kimura, N., Kirii, S., Kitaoka, Y., Kitazawa, H., Kojima, Y., Kokeyama, K., Komori, K., Kong, A., Kotake, K., Kozu, R., Kumar, R., Kuo, H-S., Kuroki, S., Kuroyanagi, S., Lee, H. K., Lee, H. M., Lee, H. W., Leonardi, M., Lin, C-Y., Lin, F-L., Liu, G. C., Marchio, M., Matsui, T., Michimura, Y., Mio, N., Miyakawa, O., Miyamoto, A., Miyoki, S., Morii, W., Morisaki, S., Moriwaki, Y., Musha, M., Nagano, S., Nagano, K., Nakamura, K., Nakamura, T., Nakano, H., Nakano, M., Narikawa, T., Quynh, L. Nguyen, Ni, W. -T., Nishizawa, A., Obuchi, Y., Oh, J., Oh, S. H., Ohashi, M., Ohishi, N., Ohkawa, M., Okutomi, K., Ono, K., Oohara, K., Ooi, C. P., Pan, S-S., Paoletti, F., Park, J., Passaquieti, R., Arellano, F. E. Peña, Sago, N., Saito, S., Saito, Y., Sakai, K., Sakai, Y., Sasai, M., Sato, S., Sato, T., Sekiguchi, T., Sekiguchi, Y., Shibata, M., Shimoda, T., Shinkai, H., Shishido, T., Shoda, A., Someya, N., Somiya, K., Son, E. J., Suemasa, A., Suzuki, T., Tagoshi, H., Tahara, H., Takahashi, H., Takahashi, R., Takeda, H., Tanaka, H., Tanaka, K., Tanaka, T., Tanioka, S., SanMartin, E. N. Tapia, Tomaru, T., Tomura, T., Travasso, F., Tsubono, K., Tsuchida, S., Uchikata, N., Uchiyama, T., Uehara, T., Ueno, K., Uraguchi, F., Ushiba, T., van Putten, M. H. P. M., Vocca, H., Wakamatsu, T., Watanabe, Y., Xu, W-R., Yamada, T., Yamamoto, K., Yamamoto, S., Yamamoto, T., Yokogawa, K., Yokoyama, J., Yokozawa, T., Yoshioka, T., Yuzurihara, H., Zeidler, S., and Zhu, Z. -H.

Subjects
Physics - Instrumentation and Detectors, General Relativity and Quantum Cosmology
Abstract

A vibration isolation system called Type-Bp system used for power recycling mirrors has been developed for KAGRA, the interferometric gravitational-wave observatory in Japan. A suspension of the Type-Bp system passively isolates an optic from seismic vibration using three main pendulum stages equipped with two vertical vibration isolation systems. A compact reaction mass around each of the main stages allows for achieving sufficient damping performance with a simple feedback as well as vibration isolation ratio. Three Type-Bp systems were installed in KAGRA, and were proved to satisfy the requirements on the damping performance, and also on estimated residual displacement of the optics.

Published
2019
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3. KAGRA: 2.5 Generation Interferometric Gravitational Wave Detector

Author

Akutsu, T., Ando, M., Arai, K., Arai, Y., Araki, S., Araya, A., Aritomi, N., Asada, H., Aso, Y., Atsuta, S., Awai, K., Bae, S., Baiotti, L., Barton, M. A., Cannon, K., Capocasa, E., Chen, C-S., Chiu, T-W., Cho, K., Chu, Y-K., Craig, K., Creus, W., Doi, K., Eda, K., Enomoto, Y., Flaminio, R., Fujii, Y., Fujimoto, M. -K., Fukunaga, M., Fukushima, M., Furuhata, T., Haino, S., Hasegawa, K., Hashino, K., Hayama, K., Hirobayashi, S., Hirose, E., Hsieh, B. H., Huang, C-Z., Ikenoue, B., Inoue, Y., Ioka, K., Itoh, Y., Izumi, K., Kaji, T., Kajita, T., Kakizaki, M., Kamiizumi, M., Kanbara, S., Kanda, N., Kanemura, S., Kaneyama, M., Kang, G., Kasuya, J., Kataoka, Y., Kawai, N., Kawamura, S., Kawasaki, T., Kim, C., Kim, J., Kim, J. C., Kim, W. S., Kim, Y. -M., Kimura, N., Kinugawa, T., Kirii, S., Kitaoka, Y., Kitazawa, H., Kojima, Y., Kokeyama, K., Komori, K., Kong, A. K. H., Kotake, K., Kozu, R., Kumar, R., Kuo, H-S., Kuroyanagi, S., Lee, H. K., Lee, H. M., Lee, H. W., Leonardi, M., Lin, C-Y., Lin, F-L., Liu, G. C., Liu, Y., Majorana, E., Mano, S., Marchio, M., Matsui, T., Matsushima, F., Michimura, Y., Mio, N., Miyakawa, O., Miyamoto, A., Miyamoto, T., Miyo, K., Miyoki, S., Morii, W., Morisaki, S., Moriwaki, Y., Morozumi, T., Musha, M., Nagano, K., Nagano, S., Nakamura, K., Nakamura, T., Nakano, H., Nakano, M., Nakao, K., Narikawa, T., Naticchioni, L., Quynh, L. Nguyen, Ni, W. -T., Nishizawa, A., Ochi, T., Oh, J. J., Oh, S. H., Ohashi, M., Ohishi, N., Ohkawa, M., Okutomi, K., Ono, K., Oohara, K., Ooi, C. P., Pan, S-S., Park, J., Arellano, F. E. Peña, Pinto, I., Sago, N., Saijo, M., Saito, Y., Sakai, K., Sakai, Y., Sasai, M., Sasaki, M., Sasaki, Y., Sato, S., Sato, T., Sekiguchi, Y., Seto, N., Shibata, M., Shimoda, T., Shinkai, H., Shishido, T., Shoda, A., Somiya, K., Son, E. J., Suemasa, A., Suzuki, T., Tagoshi, H., Tahara, H., Takahashi, H., Takahashi, R., Takamori, A., Takeda, H., Tanaka, H., Tanaka, K., Tanaka, T., Tanioka, S., Martin, E. N. Tapia San, Tatsumi, D., Tomaru, T., Tomura, T., Travasso, F., Tsubono, K., Tsuchida, S., Uchikata, N., Uchiyama, T., Uehara, T., Ueki, S., Ueno, K., Ushiba, T., van Putten, M. H. P. M., Vocca, H., Wada, S., Wakamatsu, T., Watanabe, Y., Xu, W-R., Yamada, T., Yamamoto, A., Yamamoto, K., Yamamoto, S., Yamamoto, T., Yokogawa, K., Yokoyama, J., Yokozawa, T., Yoon, T. H., Yoshioka, T., Yuzurihara, H., Zeidler, S., and Zhu, Z. -H.

Subjects
General Relativity and Quantum Cosmology, Astrophysics - Instrumentation and Methods for Astrophysics, Physics - Instrumentation and Detectors
Abstract

The recent detections of gravitational waves (GWs) reported by LIGO/Virgo collaborations have made significant impact on physics and astronomy. A global network of GW detectors will play a key role to solve the unknown nature of the sources in coordinated observations with astronomical telescopes and detectors. Here we introduce KAGRA (former name LCGT; Large-scale Cryogenic Gravitational wave Telescope), a new GW detector with two 3-km baseline arms arranged in the shape of an "L", located inside the Mt. Ikenoyama, Kamioka, Gifu, Japan. KAGRA's design is similar to those of the second generations such as Advanced LIGO/Virgo, but it will be operating at the cryogenic temperature with sapphire mirrors. This low temperature feature is advantageous for improving the sensitivity around 100 Hz and is considered as an important feature for the third generation GW detector concept (e.g. Einstein Telescope of Europe or Cosmic Explorer of USA). Hence, KAGRA is often called as a 2.5 generation GW detector based on laser interferometry. The installation and commissioning of KAGRA is underway and its cryogenic systems have been successfully tested in May, 2018. KAGRA's first observation run is scheduled in late 2019, aiming to join the third observation run (O3) of the advanced LIGO/Virgo network. In this work, we describe a brief history of KAGRA and highlights of main feature. We also discuss the prospects of GW observation with KAGRA in the era of O3. When operating along with the existing GW detectors, KAGRA will be helpful to locate a GW source more accurately and to determine the source parameters with higher precision, providing information for follow-up observations of a GW trigger candidate., Comment: 6 pages, 3 figures (quality of some figures has been reduced)

Published
2018
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6. Primary Osteoporosis

Author

Morii, H., Miki, T., Ito, M., Uchida, Y., Takaishi, Y., Nakatsuka, K., Tahara, H., Morii, Hirotoshi, editor, Nishizawa, Yoshiki, editor, and Massry, Shaul G., editor

Published
2002
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10. Highly sensitive measurement of moisture content using HTS-SQUID

Author

Tsukada, K., Kiwa, T., Tahara, H., Miyake, E., Yamada, H., Tsukamoto, A., and Kandori, A.

Subjects
Liquid nitrogen -- Thermal properties, Liquid nitrogen -- Electric properties, Magnetic fields -- Analysis, Moisture meters -- Research, Superconducting quantum interference devices -- Usage, Superconducting quantum interference devices -- Thermal properties, Business, Electronics, Electronics and electrical industries
Published
2009

32. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Author

Thery, C., Witwer, K. (Kenneth), Aikawa, E. (Elena), Alcaraz, M.J. (Maria Jose), Anderson, J.D. (Johnathon D), Andriantsitohaina, R. (Ramaroson), Antoniou, A. (Anna), Arab, T. (Tanina), Archer, F. (Fabienne), Atkin-Smith, G.K. (Georgia K), Ayre, D.C. (D Craig), Bach, J.-M. (Jean-Marie), Bachurski, D. (Daniel), Baharvand, H. (Hossein), Balaj, L. (Leonora), Baldacchino, S. (Shawn), Bauer, N.N. (Natalie N), Baxter, A.A. (Amy A), Bebawy, M. (Mary), Beckham, C. (Carla), Bedina Zavec, A. (Apolonija), Benmoussa, A. (Abderrahim), Berardi, A.C. (Anna C), Bergese, P. (Paolo), Bielska, E. (Ewa), Blenkiron, C. (Cherie), Bobis-Wozowicz, S. (Sylwia), Boilard, E. (Eric), Boireau, W. (Wilfrid), Bongiovanni, A. (Antonella), Borràs, F.E. (Francesc), Bosch, S. (Steffi), Boulanger, C.M. (Chantal), Breakefield, X. (Xandra), Breglio, A.M. (Andrew M), Brennan, M.Á. (Meadhbh Á), Brigstock, D.R. (David R), Brisson, A. (Alain), Broekman, M.L.D. (Marike), Bromberg, J.F. (Jacqueline F), Bryl-Górecka, P. (Paulina), Buch, S. (Shilpa), Buck, A.H. (Amy H), Burger, D. (Dylan), Busatto, S. (Sara), Buschmann, D. (Dominik), Bussolati, B. (Benedetta), Buzas, E. (Edit), Byrd, J.B. (James Bryan), Camussi, G. (Giovanni), Carter, D.R.F. (David RF), Caruso, S. (Sarah), Chamley, L.W. (Lawrence W), Chang, Y.-T. (Yu-Ting), Chaudhuri, A.D. (Amrita Datta), Chen, C. (Chihchen), Chen, S. (Shuai), Cheng, L. (Lesley), Chin, A.R. (Andrew R), Clayton, A. (Aled), Clerici, S.P. (Stefano P), Cocks, A. (Alex), Cocucci, E. (Emanuele), Coffey, R.J. (Robert J), Cordeiro-da-Silva, A. (Anabela), Couch, Y. (Yvonne), Coumans, F.A.W. (Frank AW), Coyle, B. (Beth), Crescitelli, R. (Rossella), Criado, M.F. (Miria Ferreira), D’Souza-Schorey, C. (Crislyn), Das, S. (Saumya), de Candia, P. (Paola), De Santana, E.F. (Eliezer F), De Wever, O. (Olivier), Del Portillo, H. (Hernando), Demaret, T. (Tanguy), Deville, S. (Sarah), Devitt, A. (Andrew), Dhondt, B. (Bert), Di Vizio, D. (Dolores), Dieterich, L.C. (Lothar C), Dolo, V. (Vincenza), Dominguez Rubio, A.P. (Ana Paula), Dominici, M. (Massimo), Dourado, M.R. (Mauricio R), Driedonks, T.A.P. (Tom AP), Duarte, F.V. (Filipe V), Duncan, H.M. (Heather M), Eichenberger, R.M. (Ramon M), Ekström, K. (Karin), EL Andaloussi, S. (Samir), Elie-Caille, C. (Celine), Erdbrügger, U. (Uta), Falcon-Perez, J.M. (Juan), Fatima, F. (Farah), Fish, J.E. (Jason E), Flores-Bellver, M. (Miguel), Försönits, A. (András), Frelet-Barrand, A. (Annie), Fricke, F. (Fabia), Fuhrmann, G. (Gregor), Gabrielsson, S. (Susanne), Gámez-Valero, A. (Ana), Gardiner, C. (Chris), Gärtner, K. (Kathrin), Gaudin, R. (Raphael), Gho, Y.S. (Yong Song), Giebel, B. (B.), Gilbert, C. (Caroline), Gimona, M. (Mario), Giusti, I. (Ilaria), Goberdhan, D.C.I. (Deborah CI), Görgens, A. (André), Gorski, S.M. (Sharon M), Greening, D.W. (David W.), Gross, J.C. (Julia Christina), Gualerzi, A. (Alice), Gupta, G.N. (Gopal N), Gustafson, D. (Dakota), Handberg, A. (Aase), Haraszti, R.A. (Reka A), Harrison, P. (Paul), Hegyesi, H. (Hargita), Hendrix, A. (An), Hill, A.F. (Andrew F), Hochberg, F.H. (Fred H), Hoffmann, K.F. (Karl F), Holder, B. (Beth), Holthofer, H. (Harry), Hosseinkhani, B. (Baharak), Hu, G. (Guoku), Huang, Y. (Yiyao), Huber, V. (Veronica), Hunt, S. (Stuart), Ibrahim, A.G.-E. (Ahmed Gamal-Eldin), Ikezu, T. (Tsuneya), Inal, J.M. (Jameel), Isin, M. (Mustafa), Ivanova, A. (Alena), Jackson, H.K. (Hannah K), Jacobsen, S. (Soren), Jay, S.M. (Steven M), Jayachandran, M. (Muthuvel), Jenster, G.W. (Guido), Jiang, L. (Lanzhou), Johnson, S.M. (Suzanne M), Jones, J.C. (Jennifer C), Jong, A. (Ambrose), Jovanovic-Talisman, T. (Tijana), Jung, S. (Stephanie), Kalluri, R. (Raghu), Kano, S.-I. (Shin-ichi), Kaur, S. (Sukhbir), Kawamura, Y. (Yumi), Keller, E.T. (Evan T), Khamari, D. (Delaram), Khomyakova, E. (Elena), Khvorova, A. (Anastasia), Kierulf, P. (Peter), Kim, K.P. (Kwang Pyo), Kislinger, T. (Thomas), Klingeborn, M. (Mikael), Klinke, D.J. (David J), Kornek, M. (Miroslaw), Kosanović, M.M. (Maja M), Kovács, Á.F. (Árpád Ferenc), Krämer-Albers, E.-M. (Eva-Maria), Krasemann, S. (Susanne), Krause, M. (Mirja), Kurochkin, I.V. (Igor V), Kusuma, G.D. (Gina D), Kuypers, S. (Sören), Laitinen, S. (Saara), Langevin, S.M. (Scott M), Languino, L.R. (Lucia R), Lannigan, J. (Joanne), Lässer, C. (Cecilia), Laurent, L.C. (Louise C), Lavieu, G. (Gregory), Lázaro-Ibáñez, E. (Elisa), Le Lay, S. (Soazig), Lee, M.-S. (Myung-Shin), Lee, Y.X.F. (Yi Xin Fiona), Lemos, D.S. (Debora S), Lenassi, M. (Metka), Leszczynska, A. (Aleksandra), Li, I.T.S. (Isaac TS), Liao, K. (Ke), Libregts, S.F. (Sten), Ligeti, E. (Erzsebet), Lim, R. (Rebecca), Lim, S.K. (Sai Kiang), Linē, A. (Aija), Linnemannstöns, K. (Karen), Llorente, A. (Alicia), Lombard, C.A. (Catherine A), Lorenowicz, M.J. (Magdalena J), Lörincz, Á.M. (Ákos M), Lötvall, J. (Jan), Lovett, J. (Jason), Lowry, M.C. (Michelle C), Loyer, X. (Xavier), Lu, Q. (Quan), Lukomska, B. (Barbara), Lunavat, T.R. (Taral R), Maas, S.L.N. (Sybren), Malhi, H. (Harmeet), Marcilla, A. (Antonio), Mariani, J. (Jacopo), Mariscal, J. (Javier), Martens-Uzunova, E.S. (Elena), Martin-Jaular, L. (Lorena), Martinez, M.C. (M Carmen), Martins, V.R. (Vilma Regina), Mathieu, M. (Mathilde), Mathivanan, S. (Suresh), Maugeri, M. (Marco), McGinnis, L.K. (Lynda K), McVey, M.J. (Mark J), Meckes, D.G. (David G), Meehan, K.L. (Katie L), Mertens, I. (Inge), Minciacchi, V.R. (Valentina R), Möller, A. (Andreas), Møller Jørgensen, M. (Malene), Morales-Kastresana, A. (Aizea), Morhayim, J. (Jess), Mullier, F. (Francois), Muraca, M. (Maurizio), Musante, L. (Luca), Mussack, V. (Veronika), Muth, D.C. (Dillon C), Myburgh, K.H. (Kathryn H), Najrana, T. (Tanbir), Nawaz, M. (Muhammad), Nazarenko, I. (Irina), Nejsum, P. (Peter), Neri, C. (Christian), Neri, T. (Tommaso), Nieuwland, C.C.M. (Carolien) van, Nimrichter, L. (Leonardo), Nolan, J.P. (John P), Nolte-’t Hoen, E.N.M. (Esther NM), Hooten, N.N. (Nicole Noren), O’Driscoll, L. (Lorraine), O’Grady, T. (Tina), O’Loghlen, A. (Ana), Ochiya, T. (Takahiro), Olivier, M. (Martin), Ortiz, A. (Alberto), Ortiz, L.A. (Luis A), Osteikoetxea, X. (Xabier), Ostegaard, O. (Ole), Ostrowski, M. (Matias), Park, J. (Jaesung), Pegtel, D.M. (D. Michiel), Peinado, H. (Hector), Perut, F. (Francesca), Pfaffl, M.W. (Michael W), Phinney, D.G. (Donald G), Pieters, B.C.H. (Bartijn CH), Pink, R.C. (Ryan C), Pisetsky, D.S. (David S), Pogge von Strandmann, E. (Elke), Polakovicova, I. (Iva), Poon, I.K.H. (Ivan KH), Powell, B.H. (Bonita H), Prada, I. (Ilaria), Pulliam, L. (Lynn), Quesenberry, P. (Peter), Radeghieri, A. (Annalisa), Raffai, R.L. (Robert L), Raimondo, S. (Stefania), Rak, J. (Janusz), Ramirez, M.I. (Marcel I.), Raposo, L. (Luís), Rayyan, M.S. (Morsi S), Regev-Rudzki, N. (Neta), Ricklefs, F.L. (Franz L), Robbins, P.D. (Paul D), Roberts, D.D. (David D), Rodrigues, S.C. (Silvia C), Rohde, E. (Eva), Rome, S. (Sophie), Rouschop, K.M.A. (Kasper MA), Rughetti, A. (Aurelia), Russell, A.E. (Ashley E), Saá, P. (Paula), Sahoo, S. (Susmita), Salas-Huenuleo, E. (Edison), Sánchez, C. (Catherine), Saugstad, J.A. (Julie A), Saul, M.J. (Meike J), Schiffelers, R.M. (Raymond), Schneider, R. (Raphael), Schøyen, T.H. (Tine Hiorth), Scott, A. (Aaron), Shahaj, E. (Eriomina), Sharma, S. (Shivani), Shatnyeva, O. (Olga), Shekari, F. (Faezeh), Shelke, G.V. (Ganesh Vilas), Shetty, A.K. (Ashok K), Shiba, K. (Kiyotaka), Siljander, P. (Pia), Silva, A.M. (Andreia M), Skowronek, A. (Agata), Snyder, O.L. (Orman L), Soares, R.P. (Rodrigo Pedro), Sódar, B.W. (Barbara W), Soekmadji, C. (Carolina), Sotillo, J. (Javier), Stahl, P.D. (Philip D), Stoorvogel, W. (Willem), Stott, S.L. (Shannon L), Strasser, E.F. (Erwin F), Swift, S. (Simon), Tahara, H. (Hidetoshi), Tewari, M. (Muneesh), Timms, K. (Kate), Tiwari, S. (Swasti), Tixeira, R. (Rochelle), Tkach, M. (Mercedes), Toh, W.S. (Wei Seong), Tomasini, R. (Richard), Torrecilhas, A.C. (Ana Claudia), Tosar, J.P. (Juan Pablo), Toxavidis, V. (Vasilis), Urbanelli, L. (Lorena), Vader, P. (Pieter), Balkom, B.W.M. (Bas) van, van der Grein, S.G. (Susanne G), Van Deun, J. (Jan), van Herwijnen, M.J.C. (Martijn JC), Van Keuren-Jensen, K. (Kendall), van Niel, G. (Guillaume), Royen, M.E. (Martin), van Wijnen, A.J. (Andre J), Vasconcelos, M.H. (M Helena), Vechetti, I.J. (Ivan J), Veit, T.D. (Tiago D), Vella, L.J. (Laura J.), Velot, É. (Émilie), Verweij, F.J. (Frederik J), Vestad, B. (Beate), Viñas, J.L. (Jose L), Visnovitz, T. (Tamás), Vukman, K.V. (Krisztina V), Wahlgren, J. (Jessica), Watson, D.C. (Dionysios C), Wauben, M.H.M. (Marca), Weaver, A. (Alissa), Webber, J.P. (Jason P), Weber, V. (Viktoria), Wehman, A.M. (Ann M), Weiss, D.J. (Daniel J), Welsh, J.A. (Joshua A), Wendt, S. (Sebastian), Wheelock, A.M. (Asa M), Wiener, Z. (Zoltán), Witte, L. (Leonie), Wolfram, J. (Joy), Xagorari, A. (Angeliki), Xander, P. (Patricia), Xu, J. (Jing), Yan, X. (Xiaomei), Yáñez-Mó, M. (María), Yin, H. (Hang), Yuana, Y., Zappulli, V. (Valentina), Zarubova, J. (Jana), Žėkas, V. (Vytautas), Zhang, J.-Y. (Jian-ye), Zhao, Z. (Zezhou), Zheng, L. (Lei), Zheutlin, A.R. (Alexander R), Zickler, A.M. (Antje M), Zimmermann, P. (Pascale), Zivkovic, A.M. (Angela M), Zocco, D. (Davide), Zuba-Surma, E.K. (Ewa K), Thery, C., Witwer, K. (Kenneth), Aikawa, E. (Elena), Alcaraz, M.J. (Maria Jose), Anderson, J.D. (Johnathon D), Andriantsitohaina, R. (Ramaroson), Antoniou, A. (Anna), Arab, T. (Tanina), Archer, F. (Fabienne), Atkin-Smith, G.K. (Georgia K), Ayre, D.C. (D Craig), Bach, J.-M. (Jean-Marie), Bachurski, D. (Daniel), Baharvand, H. (Hossein), Balaj, L. (Leonora), Baldacchino, S. (Shawn), Bauer, N.N. (Natalie N), Baxter, A.A. (Amy A), Bebawy, M. (Mary), Beckham, C. (Carla), Bedina Zavec, A. (Apolonija), Benmoussa, A. (Abderrahim), Berardi, A.C. (Anna C), Bergese, P. (Paolo), Bielska, E. (Ewa), Blenkiron, C. (Cherie), Bobis-Wozowicz, S. (Sylwia), Boilard, E. (Eric), Boireau, W. (Wilfrid), Bongiovanni, A. (Antonella), Borràs, F.E. (Francesc), Bosch, S. (Steffi), Boulanger, C.M. (Chantal), Breakefield, X. (Xandra), Breglio, A.M. (Andrew M), Brennan, M.Á. (Meadhbh Á), Brigstock, D.R. (David R), Brisson, A. (Alain), Broekman, M.L.D. (Marike), Bromberg, J.F. (Jacqueline F), Bryl-Górecka, P. (Paulina), Buch, S. (Shilpa), Buck, A.H. (Amy H), Burger, D. (Dylan), Busatto, S. (Sara), Buschmann, D. (Dominik), Bussolati, B. (Benedetta), Buzas, E. (Edit), Byrd, J.B. (James Bryan), Camussi, G. (Giovanni), Carter, D.R.F. (David RF), Caruso, S. (Sarah), Chamley, L.W. (Lawrence W), Chang, Y.-T. (Yu-Ting), Chaudhuri, A.D. (Amrita Datta), Chen, C. (Chihchen), Chen, S. (Shuai), Cheng, L. (Lesley), Chin, A.R. (Andrew R), Clayton, A. (Aled), Clerici, S.P. (Stefano P), Cocks, A. (Alex), Cocucci, E. (Emanuele), Coffey, R.J. (Robert J), Cordeiro-da-Silva, A. (Anabela), Couch, Y. (Yvonne), Coumans, F.A.W. (Frank AW), Coyle, B. (Beth), Crescitelli, R. (Rossella), Criado, M.F. (Miria Ferreira), D’Souza-Schorey, C. (Crislyn), Das, S. (Saumya), de Candia, P. (Paola), De Santana, E.F. (Eliezer F), De Wever, O. (Olivier), Del Portillo, H. (Hernando), Demaret, T. (Tanguy), Deville, S. (Sarah), Devitt, A. (Andrew), Dhondt, B. (Bert), Di Vizio, D. (Dolores), Dieterich, L.C. (Lothar C), Dolo, V. (Vincenza), Dominguez Rubio, A.P. (Ana Paula), Dominici, M. (Massimo), Dourado, M.R. (Mauricio R), Driedonks, T.A.P. (Tom AP), Duarte, F.V. (Filipe V), Duncan, H.M. (Heather M), Eichenberger, R.M. (Ramon M), Ekström, K. (Karin), EL Andaloussi, S. (Samir), Elie-Caille, C. (Celine), Erdbrügger, U. (Uta), Falcon-Perez, J.M. (Juan), Fatima, F. (Farah), Fish, J.E. (Jason E), Flores-Bellver, M. (Miguel), Försönits, A. (András), Frelet-Barrand, A. (Annie), Fricke, F. (Fabia), Fuhrmann, G. (Gregor), Gabrielsson, S. (Susanne), Gámez-Valero, A. (Ana), Gardiner, C. (Chris), Gärtner, K. (Kathrin), Gaudin, R. (Raphael), Gho, Y.S. (Yong Song), Giebel, B. (B.), Gilbert, C. (Caroline), Gimona, M. (Mario), Giusti, I. (Ilaria), Goberdhan, D.C.I. (Deborah CI), Görgens, A. (André), Gorski, S.M. (Sharon M), Greening, D.W. (David W.), Gross, J.C. (Julia Christina), Gualerzi, A. (Alice), Gupta, G.N. (Gopal N), Gustafson, D. (Dakota), Handberg, A. (Aase), Haraszti, R.A. (Reka A), Harrison, P. (Paul), Hegyesi, H. (Hargita), Hendrix, A. (An), Hill, A.F. (Andrew F), Hochberg, F.H. (Fred H), Hoffmann, K.F. (Karl F), Holder, B. (Beth), Holthofer, H. (Harry), Hosseinkhani, B. (Baharak), Hu, G. (Guoku), Huang, Y. (Yiyao), Huber, V. (Veronica), Hunt, S. (Stuart), Ibrahim, A.G.-E. (Ahmed Gamal-Eldin), Ikezu, T. (Tsuneya), Inal, J.M. (Jameel), Isin, M. (Mustafa), Ivanova, A. (Alena), Jackson, H.K. (Hannah K), Jacobsen, S. (Soren), Jay, S.M. (Steven M), Jayachandran, M. (Muthuvel), Jenster, G.W. (Guido), Jiang, L. (Lanzhou), Johnson, S.M. (Suzanne M), Jones, J.C. (Jennifer C), Jong, A. (Ambrose), Jovanovic-Talisman, T. (Tijana), Jung, S. (Stephanie), Kalluri, R. (Raghu), Kano, S.-I. (Shin-ichi), Kaur, S. (Sukhbir), Kawamura, Y. (Yumi), Keller, E.T. (Evan T), Khamari, D. (Delaram), Khomyakova, E. (Elena), Khvorova, A. (Anastasia), Kierulf, P. (Peter), Kim, K.P. (Kwang Pyo), Kislinger, T. (Thomas), Klingeborn, M. (Mikael), Klinke, D.J. (David J), Kornek, M. (Miroslaw), Kosanović, M.M. (Maja M), Kovács, Á.F. (Árpád Ferenc), Krämer-Albers, E.-M. (Eva-Maria), Krasemann, S. (Susanne), Krause, M. (Mirja), Kurochkin, I.V. (Igor V), Kusuma, G.D. (Gina D), Kuypers, S. (Sören), Laitinen, S. (Saara), Langevin, S.M. (Scott M), Languino, L.R. (Lucia R), Lannigan, J. (Joanne), Lässer, C. (Cecilia), Laurent, L.C. (Louise C), Lavieu, G. (Gregory), Lázaro-Ibáñez, E. (Elisa), Le Lay, S. (Soazig), Lee, M.-S. (Myung-Shin), Lee, Y.X.F. (Yi Xin Fiona), Lemos, D.S. (Debora S), Lenassi, M. (Metka), Leszczynska, A. (Aleksandra), Li, I.T.S. (Isaac TS), Liao, K. (Ke), Libregts, S.F. (Sten), Ligeti, E. (Erzsebet), Lim, R. (Rebecca), Lim, S.K. (Sai Kiang), Linē, A. (Aija), Linnemannstöns, K. (Karen), Llorente, A. (Alicia), Lombard, C.A. (Catherine A), Lorenowicz, M.J. (Magdalena J), Lörincz, Á.M. (Ákos M), Lötvall, J. (Jan), Lovett, J. (Jason), Lowry, M.C. (Michelle C), Loyer, X. (Xavier), Lu, Q. (Quan), Lukomska, B. (Barbara), Lunavat, T.R. (Taral R), Maas, S.L.N. (Sybren), Malhi, H. (Harmeet), Marcilla, A. (Antonio), Mariani, J. (Jacopo), Mariscal, J. (Javier), Martens-Uzunova, E.S. (Elena), Martin-Jaular, L. (Lorena), Martinez, M.C. (M Carmen), Martins, V.R. (Vilma Regina), Mathieu, M. (Mathilde), Mathivanan, S. (Suresh), Maugeri, M. (Marco), McGinnis, L.K. (Lynda K), McVey, M.J. (Mark J), Meckes, D.G. (David G), Meehan, K.L. (Katie L), Mertens, I. (Inge), Minciacchi, V.R. (Valentina R), Möller, A. (Andreas), Møller Jørgensen, M. (Malene), Morales-Kastresana, A. (Aizea), Morhayim, J. (Jess), Mullier, F. (Francois), Muraca, M. (Maurizio), Musante, L. (Luca), Mussack, V. (Veronika), Muth, D.C. (Dillon C), Myburgh, K.H. (Kathryn H), Najrana, T. (Tanbir), Nawaz, M. (Muhammad), Nazarenko, I. (Irina), Nejsum, P. (Peter), Neri, C. (Christian), Neri, T. (Tommaso), Nieuwland, C.C.M. (Carolien) van, Nimrichter, L. (Leonardo), Nolan, J.P. (John P), Nolte-’t Hoen, E.N.M. (Esther NM), Hooten, N.N. (Nicole Noren), O’Driscoll, L. (Lorraine), O’Grady, T. (Tina), O’Loghlen, A. (Ana), Ochiya, T. (Takahiro), Olivier, M. (Martin), Ortiz, A. (Alberto), Ortiz, L.A. (Luis A), Osteikoetxea, X. (Xabier), Ostegaard, O. (Ole), Ostrowski, M. (Matias), Park, J. (Jaesung), Pegtel, D.M. (D. Michiel), Peinado, H. (Hector), Perut, F. (Francesca), Pfaffl, M.W. (Michael W), Phinney, D.G. (Donald G), Pieters, B.C.H. (Bartijn CH), Pink, R.C. (Ryan C), Pisetsky, D.S. (David S), Pogge von Strandmann, E. (Elke), Polakovicova, I. (Iva), Poon, I.K.H. (Ivan KH), Powell, B.H. (Bonita H), Prada, I. (Ilaria), Pulliam, L. (Lynn), Quesenberry, P. (Peter), Radeghieri, A. (Annalisa), Raffai, R.L. (Robert L), Raimondo, S. (Stefania), Rak, J. (Janusz), Ramirez, M.I. (Marcel I.), Raposo, L. (Luís), Rayyan, M.S. (Morsi S), Regev-Rudzki, N. (Neta), Ricklefs, F.L. (Franz L), Robbins, P.D. (Paul D), Roberts, D.D. (David D), Rodrigues, S.C. (Silvia C), Rohde, E. (Eva), Rome, S. (Sophie), Rouschop, K.M.A. (Kasper MA), Rughetti, A. (Aurelia), Russell, A.E. (Ashley E), Saá, P. (Paula), Sahoo, S. (Susmita), Salas-Huenuleo, E. (Edison), Sánchez, C. (Catherine), Saugstad, J.A. (Julie A), Saul, M.J. (Meike J), Schiffelers, R.M. (Raymond), Schneider, R. (Raphael), Schøyen, T.H. (Tine Hiorth), Scott, A. (Aaron), Shahaj, E. (Eriomina), Sharma, S. (Shivani), Shatnyeva, O. (Olga), Shekari, F. (Faezeh), Shelke, G.V. (Ganesh Vilas), Shetty, A.K. (Ashok K), Shiba, K. (Kiyotaka), Siljander, P. (Pia), Silva, A.M. (Andreia M), Skowronek, A. (Agata), Snyder, O.L. (Orman L), Soares, R.P. (Rodrigo Pedro), Sódar, B.W. (Barbara W), Soekmadji, C. (Carolina), Sotillo, J. (Javier), Stahl, P.D. (Philip D), Stoorvogel, W. (Willem), Stott, S.L. (Shannon L), Strasser, E.F. (Erwin F), Swift, S. (Simon), Tahara, H. (Hidetoshi), Tewari, M. (Muneesh), Timms, K. (Kate), Tiwari, S. (Swasti), Tixeira, R. (Rochelle), Tkach, M. (Mercedes), Toh, W.S. (Wei Seong), Tomasini, R. (Richard), Torrecilhas, A.C. (Ana Claudia), Tosar, J.P. (Juan Pablo), Toxavidis, V. (Vasilis), Urbanelli, L. (Lorena), Vader, P. (Pieter), Balkom, B.W.M. (Bas) van, van der Grein, S.G. (Susanne G), Van Deun, J. (Jan), van Herwijnen, M.J.C. (Martijn JC), Van Keuren-Jensen, K. (Kendall), van Niel, G. (Guillaume), Royen, M.E. (Martin), van Wijnen, A.J. (Andre J), Vasconcelos, M.H. (M Helena), Vechetti, I.J. (Ivan J), Veit, T.D. (Tiago D), Vella, L.J. (Laura J.), Velot, É. (Émilie), Verweij, F.J. (Frederik J), Vestad, B. (Beate), Viñas, J.L. (Jose L), Visnovitz, T. (Tamás), Vukman, K.V. (Krisztina V), Wahlgren, J. (Jessica), Watson, D.C. (Dionysios C), Wauben, M.H.M. (Marca), Weaver, A. (Alissa), Webber, J.P. (Jason P), Weber, V. (Viktoria), Wehman, A.M. (Ann M), Weiss, D.J. (Daniel J), Welsh, J.A. (Joshua A), Wendt, S. (Sebastian), Wheelock, A.M. (Asa M), Wiener, Z. (Zoltán), Witte, L. (Leonie), Wolfram, J. (Joy), Xagorari, A. (Angeliki), Xander, P. (Patricia), Xu, J. (Jing), Yan, X. (Xiaomei), Yáñez-Mó, M. (María), Yin, H. (Hang), Yuana, Y., Zappulli, V. (Valentina), Zarubova, J. (Jana), Žėkas, V. (Vytautas), Zhang, J.-Y. (Jian-ye), Zhao, Z. (Zezhou), Zheng, L. (Lei), Zheutlin, A.R. (Alexander R), Zickler, A.M. (Antje M), Zimmermann, P. (Pascale), Zivkovic, A.M. (Angela M), Zocco, D. (Davide), and Zuba-Surma, E.K. (Ewa K)

Abstract

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make the

Published
2019
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38. Vibration isolation system with a compact damping system for power recycling mirrors of KAGRA

Author

Akiyama, Y, primary, Akutsu, T, additional, Ando, M, additional, Arai, K, additional, Arai, Y, additional, Araki, S, additional, Araya, A, additional, Aritomi, N, additional, Asada, H, additional, Aso, Y, additional, Bae, S, additional, Baiotti, L, additional, Barton, M A, additional, Cannon, K, additional, Capocasa, E, additional, Chen, C-S, additional, Chiu, T-W, additional, Cho, K, additional, Chu, Y-K, additional, Craig, K, additional, Dattilo, V, additional, Doi, K, additional, Enomoto, Y, additional, Flaminio, R, additional, Fujii, Y, additional, Fujimoto, M-K, additional, Fukunaga, M, additional, Fukushima, M, additional, Furuhata, T, additional, Haino, S, additional, Hasegawa, K, additional, Hashimoto, Y, additional, Hashino, K, additional, Hayama, K, additional, Hirayama, T, additional, Hirose, E, additional, Hsieh, B H, additional, Huang, C-Z, additional, Ikenoue, B, additional, Inoue, Y, additional, Ioka, K, additional, Itoh, Y, additional, Izumi, K, additional, Kaji, T, additional, Kajita, T, additional, Kakizaki, M, additional, Kamiizumi, M, additional, Kanbara, S, additional, Kanda, N, additional, Kanemura, S, additional, Kang, G, additional, Kasuya, J, additional, Kawai, N, additional, Kawasaki, T, additional, Kim, C, additional, Kim, W S, additional, Kim, J, additional, Kim, J C, additional, Kimura, N, additional, Kirii, S, additional, Kitaoka, Y, additional, Kitazawa, H, additional, Kojima, Y, additional, Kokeyama, K, additional, Komori, K, additional, Kong, A, additional, Kotake, K, additional, Kozu, R, additional, Kumar, R, additional, Kuo, H-S, additional, Kuroki, S, additional, Kuroyanagi, S, additional, Lee, H K, additional, Lee, H M, additional, Lee, H W, additional, Leonardi, M, additional, Lin, C-Y, additional, Lin, F-L, additional, Liu, G C, additional, Marchio, M, additional, Matsui, T, additional, Michimura, Y, additional, Mio, N, additional, Miyakawa, O, additional, Miyamoto, A, additional, Miyoki, S, additional, Morii, W, additional, Morisaki, S, additional, Moriwaki, Y, additional, Musha, M, additional, Nagano, S, additional, Nagano, K, additional, Nakamura, K, additional, Nakamura, T, additional, Nakano, H, additional, Nakano, M, additional, Narikawa, T, additional, Nguyen Quynh, L, additional, Ni, W-T, additional, Nishizawa, A, additional, Obuchi, Y, additional, Oh, J, additional, Oh, S H, additional, Ohashi, M, additional, Ohishi, N, additional, Ohkawa, M, additional, Okutomi, K, additional, Ono, K, additional, Oohara, K, additional, Ooi, C P, additional, Pan, S-S, additional, Paoletti, F, additional, Park, J, additional, Passaquieti, R, additional, Peña Arellano, F E, additional, Sago, N, additional, Saito, S, additional, Saito, Y, additional, Sakai, K, additional, Sakai, Y, additional, Sasai, M, additional, Sato, S, additional, Sato, T, additional, Sekiguchi, T, additional, Sekiguchi, Y, additional, Shibata, M, additional, Shimoda, T, additional, Shinkai, H, additional, Shishido, T, additional, Shoda, A, additional, Someya, N, additional, Somiya, K, additional, Son, E J, additional, Suemasa, A, additional, Suzuki, T, additional, Tagoshi, H, additional, Tahara, H, additional, Takahashi, H, additional, Takahashi, R, additional, Takeda, H, additional, Tanaka, H, additional, Tanaka, K, additional, Tanaka, T, additional, Tanioka, S, additional, Tapia San Martin, E N, additional, Tomaru, T, additional, Tomura, T, additional, Travasso, F, additional, Tsubono, K, additional, Tsuchida, S, additional, Uchikata, N, additional, Uchiyama, T, additional, Uehara, T, additional, Ueno, K, additional, Uraguchi, F, additional, Ushiba, T, additional, van Putten, M H P M, additional, Vocca, H, additional, Wakamatsu, T, additional, Watanabe, Y, additional, Xu, W-R, additional, Yamada, T, additional, Yamamoto, K, additional, Yamamoto, S, additional, Yamamoto, T, additional, Yokogawa, K, additional, Yokoyama, J, additional, Yokozawa, T, additional, Yoshioka, T, additional, Yuzurihara, H, additional, Zeidler, S, additional, and Zhu, Z-H, additional

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2019
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42. Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper

Author

Mateescu, B., Kowal, E., van Balkom, B., Bartel, S., Bhattacharyya, S., Buzá, E., Buck, A., de Candia, P., Chow, F., Das, S., Driedonks, T., Fernández-Messina, L., Haderk, F., Hil, L, A., Jones, J., Van Keuren-Jensen, K., Lai, C., Lässer, C., Liegro, D., I, Lunavat, T., Lorenowicz, M., Maas, S., Mäger, I., Mittelbrunn, M., Momma, S., Mukherjee, K., Nawaz, M., Pegtel, D., Pfaffl, M., Schiffelers, R., Tahara, H., Théry, C., Tosar, J., Wauben, M., Witwer, K., Nolte-'t Hoen, E., Mateescu, B, Kowal, EJ, van Balkom, BW, Bartel, S, Bhattacharyya, SN, Buzá, EI, Buck, AH, de Candia, P, Chow, FW, Das, S, Driedonks, TA, Fernández-Messina, L, Haderk, F, Hil,l AF, Jones, JC, Van Keuren-Jensen, KR, Lai, CP, Lässer, C, Di Liegro, I, Lunavat, TR, Lorenowicz, MJ, Maas, S, Mäger,I, Mittelbrunn, M, Momma, S, Mukherjee, K, Nawaz, M, Pegtel, DM, Pfaffl, MW, Schiffelers, RM, Tahara, H, Théry, C, Tosar, JP, Wauben, MH, Witwer, KW, Nolte-'t Hoen, EN., Mateescu, B., Kowal, E. J. K., van Balkom, B. W. M., Bartel, S., Bhattacharyya, S. N., Buzas, E. I., Buck, A. H., de Candia, P., Chow, F. W. N., Das, S., Driedonks, T. A. P., Fernandez-Messina, L., Haderk, F., Hill, A. F., Jones, J. C., Van Keuren-Jensen, K. R., Lai, C. P., Lasser, C., di Liegro, I., Lunavat, T. R., Lorenowicz, M. J., Maas, S. L. N., Mager, I., Mittelbrunn, M., Momma, S., Mukherjee, K., Nawaz, M., Pegtel, D. M., Pfaffl, M. W., Schiffelers, R. M., Tahara, H., Thery, C., Tosar, J. P., Wauben, M. H. M., Witwer, K. W., Nolte-'t Hoen, E. N. M., dB&C I&I, LS Celbiologie-Algemeen, CCA - Imaging and biomarkers, Amsterdam Neuroscience - Cellular & Molecular Mechanisms, and Pathology

Subjects
0301 basic medicine, Histology, media_common.quotation_subject, mRNA, non-coding RNA, exosomes, Biology, Exosomes, Article, 03 medical and health sciences, Quantification, Settore BIO/10 - Biochimica, Journal Article, MRNA, exosome, Original Research Article, ddc:610, Function, lcsh:QH573-671, Function (engineering), Non-coding RNA, media_common, function, lcsh:Cytology, Sorting, RNA, Extracellular vesicle, Cell Biology, Extracellular vesicles, Data science, RNA binding protein, quantification, RNA binding proteins, sorting, Cell biology, Variety (cybernetics), 030104 developmental biology, Signalling, Position paper, Functional analysis (psychology)
Abstract

The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data., Journal of Extracellular Vesicles, 6 (1), ISSN:2001-3078

Published
2017

43. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017

Author

Clayton, A. (Aled), Buschmann, D. (Dominik), Byrd, J.B. (J. Brian), Carter, D.R.F. (David R. F.), Cheng, L. (Lesley), Compton, C. (Carolyn), Daaboul, G. (George), Devitt, A. (Andrew), Falcon-Perez, J.M. (Juan), Gardiner, C. (Chris), Gustafson, D. (Dakota), Harrison, P. (Paul), Helmbrecht, C. (Clemens), Hendrix, A. (An), Hill, A.F. (Andrew), Hoffman, A. (Andrew), Jones, J.C. (Jennifer C.), Kalluri, R. (Raghu), Kang, J.Y. (Ji Yoon), Kirchner, B. (Benedikt), Lässer, C. (Cecilia), Lawson, C. (Charlotte), Lenassi, M. (Metka), Levin, C. (Carina), Llorente, A. (Alicia), Martens-Uzunova, E.S. (Elena), Möller, A. (Andreas), Musante, L. (Luca), Ochiya, T. (Takahiro), Pink, R.C. (Ryan C), Tahara, H. (Hidetoshi), Wauben, M.H.M. (Marca), Webber, J.P. (Jason P.), Welsh, J.A. (Joshua A.), Witwer, K. (Kenneth), Yin, H. (Hang), Nieuwland, C.C.M. (Carolien) van, Clayton, A. (Aled), Buschmann, D. (Dominik), Byrd, J.B. (J. Brian), Carter, D.R.F. (David R. F.), Cheng, L. (Lesley), Compton, C. (Carolyn), Daaboul, G. (George), Devitt, A. (Andrew), Falcon-Perez, J.M. (Juan), Gardiner, C. (Chris), Gustafson, D. (Dakota), Harrison, P. (Paul), Helmbrecht, C. (Clemens), Hendrix, A. (An), Hill, A.F. (Andrew), Hoffman, A. (Andrew), Jones, J.C. (Jennifer C.), Kalluri, R. (Raghu), Kang, J.Y. (Ji Yoon), Kirchner, B. (Benedikt), Lässer, C. (Cecilia), Lawson, C. (Charlotte), Lenassi, M. (Metka), Levin, C. (Carina), Llorente, A. (Alicia), Martens-Uzunova, E.S. (Elena), Möller, A. (Andreas), Musante, L. (Luca), Ochiya, T. (Takahiro), Pink, R.C. (Ryan C), Tahara, H. (Hidetoshi), Wauben, M.H.M. (Marca), Webber, J.P. (Jason P.), Welsh, J.A. (Joshua A.), Witwer, K. (Kenneth), Yin, H. (Hang), and Nieuwland, C.C.M. (Carolien) van

Abstract

This report summarises the presentations and activities of the ISEV Workshop on extracellular vesicle biomarkers held in Birmingham, UK during December 2017. Among the key messages was broad agreement about the importance of biospecimen science. Much greater attention needs to be paid towards the provenance of collected samples. The workshop also highlighted clear gaps in our knowledge about pre-analytical factors that alter extracellular vesicles (EVs). The future utility of certified standards for credentialing of instruments and software, to analyse EV and for tracking the influence of isolation steps on the structure and content of EVs were also discussed. Several example studies were presented, demonstrating the potential utility for EVs in disease diagnosis, prognosis, longitudinal serial testing and stratification of patients. The conclusion of the workshop was that more effort focused on pre-analytical issues and benchmarking of isolation methods is needed to strengthen collaborations and advance more effective biomarkers.

Published
2018
Full Text
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44. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Author

Thery, C, Witwer, KW, Aikawa, E, Jose Alcaraz, M, Anderson, JD, Andriantsitohaina, R, Antoniou, A, Arab, T, Archer, F, Atkin-Smith, GK, Ayre, DC, Bach, J-M, Bachurski, D, Baharvand, H, Balaj, L, Baldacchino, S, Bauer, NN, Baxter, AA, Bebawy, M, Beckham, C, Zavec, AB, Benmoussa, A, Berardi, AC, Bergese, P, Bielska, E, Blenkiron, C, Bobis-Wozowicz, S, Boilard, E, Boireau, W, Bongiovanni, A, Borras, FE, Bosch, S, Boulanger, CM, Breakefield, X, Breglio, AM, Brennan, MA, Brigstock, DR, Brisson, A, Broekman, MLD, Bromberg, JF, Bryl-Gorecka, P, Buch, S, Buck, AH, Burger, D, Busatto, S, Buschmann, D, Bussolati, B, Buzas, E, Byrd, JB, Camussi, G, Carter, DRF, Caruso, S, Chamley, LW, Chang, Y-T, Chen, C, Chen, S, Cheng, L, Chin, AR, Clayton, A, Clerici, SP, Cocks, A, Cocucci, E, Coffey, RJ, Cordeiro-da-Silva, A, Couch, Y, Coumans, FAW, Coyle, B, Crescitelli, R, Criado, MF, D'Souza-Schorey, C, Das, S, Chaudhuri, AD, de Candia, P, De Santana Junior, EF, De Wever, O, del Portillo, HA, Demaret, T, Deville, S, Devitt, A, Dhondt, B, Di Vizio, D, Dieterich, LC, Dolo, V, Dominguez Rubio, AP, Dominici, M, Dourado, MR, Driedonks, TAP, Duarte, F, Duncan, HM, Eichenberger, RM, Ekstrom, K, Andaloussi, SEL, Elie-Caille, C, Erdbrugger, U, Falcon-Perez, JM, Fatima, F, Fish, JE, Flores-Bellver, M, Forsonits, A, Frelet-Barrand, A, Fricke, F, Fuhrmann, G, Gabrielsson, S, Gamez-Valero, A, Gardiner, C, Gaertner, K, Gaudin, R, Gho, YS, Giebel, B, Gilbert, C, Gimona, M, Giusti, I, Goberdhan, DC, Goergens, A, Gorski, SM, Greening, DW, Gross, JC, Gualerzi, A, Gupta, GN, Gustafson, D, Handberg, A, Haraszti, RA, Harrison, P, Hegyesi, H, Hendrix, A, Hill, AF, Hochberg, FH, Hoffmann, KF, Holder, B, Holthofer, H, Hosseinkhani, B, Hu, G, Huang, Y, Huber, V, Hunt, S, Ibrahim, AG-E, Ikezu, T, Inal, JM, Isin, M, Ivanova, A, Jackson, HK, Jacobsen, S, Jay, SM, Jayachandran, M, Jenster, G, Jiang, L, Johnson, SM, Jones, JC, Jong, A, Jovanovic-Talisman, T, Jung, S, Kalluri, R, Kano, S-I, Kaur, S, Kawamura, Y, Keller, ET, Khamari, D, Khomyakova, E, Khvorova, A, Kierulf, P, Kim, KP, Kislinger, T, Klingeborn, M, Klinke, DJ, Kornek, M, Kosanovic, MM, Kovacs, AF, Kraemer-Albers, E-M, Krasemann, S, Krause, M, Kurochkin, I, Kusuma, GD, Kuypers, S, Laitinen, S, Langevin, SM, Languino, LR, Lannigan, J, Lasser, C, Laurent, LC, Lavieu, G, Lazaro-Ibanez, E, Le Lay, S, Lee, M-S, Lee, YXF, Lemos, DS, Lenassi, M, Leszczynska, A, Li, ITS, Liao, K, Libregts, SF, Ligeti, E, Lim, R, Lim, SK, Line, A, Linnemannstoens, K, Llorente, A, Lombard, CA, Lorenowicz, MJ, Lorincz, AM, Lotvall, J, Lovett, J, Lowry, MC, Loyer, X, Lu, Q, Lukomska, B, Lunavat, TR, Maas, SLN, Malhi, H, Marcilla, A, Mariani, J, Mariscal, J, Martens-Uzunova, ES, Martin-Jaular, L, Martinez, MC, Martins, VR, Mathieu, M, Mathivanan, S, Maugeri, M, McGinnis, LK, McVey, MJ, Meckes, DG, Meehan, KL, Mertens, I, Minciacchi, VR, Moller, A, Jorgensen, MM, Morales-Kastresana, A, Morhayim, J, Mullier, F, Muraca, M, Musante, L, Mussack, V, Muth, DC, Myburgh, KH, Najrana, T, Nawaz, M, Nazarenko, I, Nejsum, P, Neri, C, Neri, T, Nieuwland, R, Nimrichter, L, Nolan, JP, Nolte-'t Hoen, ENM, Noren Hooten, N, O'Driscoll, L, O'Grady, T, O'Loghlen, A, Ochiya, T, Olivier, M, Ortiz, A, Ortiz, LA, Osteikoetxea, X, Ostegaard, O, Ostrowski, M, Park, J, Pegtel, DM, Peinado, H, Perut, F, Pfaffl, MW, Phinney, DG, Pieters, BCH, Pink, RC, Pisetsky, DS, von Strandmann, EP, Polakovicova, I, Poon, IKH, Powell, BH, Prada, I, Pulliam, L, Quesenberry, P, Radeghieri, A, Raffai, RL, Raimondo, S, Rak, J, Ramirez, M, Raposo, G, Rayyan, MS, Regev-Rudzki, N, Ricklefs, FL, Robbins, PD, Roberts, DD, Rodrigues, SC, Rohde, E, Rome, S, Rouschop, KMA, Rughetti, A, Russell, AE, Saa, P, Sahoo, S, Salas-Huenuleo, E, Sanchez, C, Saugstad, JA, Saul, MJ, Schiffelers, RM, Schneider, R, Schoyen, TH, Scott, A, Shahaj, E, Sharma, S, Shatnyeva, O, Shekari, F, Shelke, GV, Shetty, AK, Shiba, K, Siljander, PR-M, Silva, AM, Skowronek, A, Snyder, OL, Soares, RP, Sodar, BW, Soekmadji, C, Sotillo, J, Stahl, PD, Stoorvogel, W, Stott, SL, Strasser, EF, Swift, S, Tahara, H, Tewari, M, Timms, K, Tiwari, S, Tixeira, R, Tkach, M, Toh, WS, Tomasini, R, Torrecilhas, AC, Pablo Tosar, J, Toxavidis, V, Urbanelli, L, Vader, P, van Balkom, BWM, van der Grein, SG, Van Deun, J, van Herwijnen, MJC, Van Keuren-Jensen, K, van Niel, G, van Royen, ME, van Wijnen, AJ, Helena Vasconcelos, M, Vechetti, IJ, Veit, TD, Vella, LJ, Velot, E, Verweij, FJ, Vestad, B, Vinas, JL, Visnovitz, T, Vukman, KV, Wahlgren, J, Watson, DC, Wauben, MHM, Weaver, A, Webber, JP, Weber, V, Wehman, AM, Weiss, DJ, Welsh, JA, Wendt, S, Wheelock, AM, Wiener, Z, Witte, L, Wolfram, J, Xagorari, A, Xander, P, Xu, J, Yan, X, Yanez-Mo, M, Yin, H, Yuana, Y, Zappulli, V, Zarubova, J, Zekas, V, Zhang, J-Y, Zhao, Z, Zheng, L, Zheutlin, AR, Zickler, AM, Zimmermann, P, Zivkovic, AM, Zocco, D, Zuba-Surma, EK, Thery, C, Witwer, KW, Aikawa, E, Jose Alcaraz, M, Anderson, JD, Andriantsitohaina, R, Antoniou, A, Arab, T, Archer, F, Atkin-Smith, GK, Ayre, DC, Bach, J-M, Bachurski, D, Baharvand, H, Balaj, L, Baldacchino, S, Bauer, NN, Baxter, AA, Bebawy, M, Beckham, C, Zavec, AB, Benmoussa, A, Berardi, AC, Bergese, P, Bielska, E, Blenkiron, C, Bobis-Wozowicz, S, Boilard, E, Boireau, W, Bongiovanni, A, Borras, FE, Bosch, S, Boulanger, CM, Breakefield, X, Breglio, AM, Brennan, MA, Brigstock, DR, Brisson, A, Broekman, MLD, Bromberg, JF, Bryl-Gorecka, P, Buch, S, Buck, AH, Burger, D, Busatto, S, Buschmann, D, Bussolati, B, Buzas, E, Byrd, JB, Camussi, G, Carter, DRF, Caruso, S, Chamley, LW, Chang, Y-T, Chen, C, Chen, S, Cheng, L, Chin, AR, Clayton, A, Clerici, SP, Cocks, A, Cocucci, E, Coffey, RJ, Cordeiro-da-Silva, A, Couch, Y, Coumans, FAW, Coyle, B, Crescitelli, R, Criado, MF, D'Souza-Schorey, C, Das, S, Chaudhuri, AD, de Candia, P, De Santana Junior, EF, De Wever, O, del Portillo, HA, Demaret, T, Deville, S, Devitt, A, Dhondt, B, Di Vizio, D, Dieterich, LC, Dolo, V, Dominguez Rubio, AP, Dominici, M, Dourado, MR, Driedonks, TAP, Duarte, F, Duncan, HM, Eichenberger, RM, Ekstrom, K, Andaloussi, SEL, Elie-Caille, C, Erdbrugger, U, Falcon-Perez, JM, Fatima, F, Fish, JE, Flores-Bellver, M, Forsonits, A, Frelet-Barrand, A, Fricke, F, Fuhrmann, G, Gabrielsson, S, Gamez-Valero, A, Gardiner, C, Gaertner, K, Gaudin, R, Gho, YS, Giebel, B, Gilbert, C, Gimona, M, Giusti, I, Goberdhan, DC, Goergens, A, Gorski, SM, Greening, DW, Gross, JC, Gualerzi, A, Gupta, GN, Gustafson, D, Handberg, A, Haraszti, RA, Harrison, P, Hegyesi, H, Hendrix, A, Hill, AF, Hochberg, FH, Hoffmann, KF, Holder, B, Holthofer, H, Hosseinkhani, B, Hu, G, Huang, Y, Huber, V, Hunt, S, Ibrahim, AG-E, Ikezu, T, Inal, JM, Isin, M, Ivanova, A, Jackson, HK, Jacobsen, S, Jay, SM, Jayachandran, M, Jenster, G, Jiang, L, Johnson, SM, Jones, JC, Jong, A, Jovanovic-Talisman, T, Jung, S, Kalluri, R, Kano, S-I, Kaur, S, Kawamura, Y, Keller, ET, Khamari, D, Khomyakova, E, Khvorova, A, Kierulf, P, Kim, KP, Kislinger, T, Klingeborn, M, Klinke, DJ, Kornek, M, Kosanovic, MM, Kovacs, AF, Kraemer-Albers, E-M, Krasemann, S, Krause, M, Kurochkin, I, Kusuma, GD, Kuypers, S, Laitinen, S, Langevin, SM, Languino, LR, Lannigan, J, Lasser, C, Laurent, LC, Lavieu, G, Lazaro-Ibanez, E, Le Lay, S, Lee, M-S, Lee, YXF, Lemos, DS, Lenassi, M, Leszczynska, A, Li, ITS, Liao, K, Libregts, SF, Ligeti, E, Lim, R, Lim, SK, Line, A, Linnemannstoens, K, Llorente, A, Lombard, CA, Lorenowicz, MJ, Lorincz, AM, Lotvall, J, Lovett, J, Lowry, MC, Loyer, X, Lu, Q, Lukomska, B, Lunavat, TR, Maas, SLN, Malhi, H, Marcilla, A, Mariani, J, Mariscal, J, Martens-Uzunova, ES, Martin-Jaular, L, Martinez, MC, Martins, VR, Mathieu, M, Mathivanan, S, Maugeri, M, McGinnis, LK, McVey, MJ, Meckes, DG, Meehan, KL, Mertens, I, Minciacchi, VR, Moller, A, Jorgensen, MM, Morales-Kastresana, A, Morhayim, J, Mullier, F, Muraca, M, Musante, L, Mussack, V, Muth, DC, Myburgh, KH, Najrana, T, Nawaz, M, Nazarenko, I, Nejsum, P, Neri, C, Neri, T, Nieuwland, R, Nimrichter, L, Nolan, JP, Nolte-'t Hoen, ENM, Noren Hooten, N, O'Driscoll, L, O'Grady, T, O'Loghlen, A, Ochiya, T, Olivier, M, Ortiz, A, Ortiz, LA, Osteikoetxea, X, Ostegaard, O, Ostrowski, M, Park, J, Pegtel, DM, Peinado, H, Perut, F, Pfaffl, MW, Phinney, DG, Pieters, BCH, Pink, RC, Pisetsky, DS, von Strandmann, EP, Polakovicova, I, Poon, IKH, Powell, BH, Prada, I, Pulliam, L, Quesenberry, P, Radeghieri, A, Raffai, RL, Raimondo, S, Rak, J, Ramirez, M, Raposo, G, Rayyan, MS, Regev-Rudzki, N, Ricklefs, FL, Robbins, PD, Roberts, DD, Rodrigues, SC, Rohde, E, Rome, S, Rouschop, KMA, Rughetti, A, Russell, AE, Saa, P, Sahoo, S, Salas-Huenuleo, E, Sanchez, C, Saugstad, JA, Saul, MJ, Schiffelers, RM, Schneider, R, Schoyen, TH, Scott, A, Shahaj, E, Sharma, S, Shatnyeva, O, Shekari, F, Shelke, GV, Shetty, AK, Shiba, K, Siljander, PR-M, Silva, AM, Skowronek, A, Snyder, OL, Soares, RP, Sodar, BW, Soekmadji, C, Sotillo, J, Stahl, PD, Stoorvogel, W, Stott, SL, Strasser, EF, Swift, S, Tahara, H, Tewari, M, Timms, K, Tiwari, S, Tixeira, R, Tkach, M, Toh, WS, Tomasini, R, Torrecilhas, AC, Pablo Tosar, J, Toxavidis, V, Urbanelli, L, Vader, P, van Balkom, BWM, van der Grein, SG, Van Deun, J, van Herwijnen, MJC, Van Keuren-Jensen, K, van Niel, G, van Royen, ME, van Wijnen, AJ, Helena Vasconcelos, M, Vechetti, IJ, Veit, TD, Vella, LJ, Velot, E, Verweij, FJ, Vestad, B, Vinas, JL, Visnovitz, T, Vukman, KV, Wahlgren, J, Watson, DC, Wauben, MHM, Weaver, A, Webber, JP, Weber, V, Wehman, AM, Weiss, DJ, Welsh, JA, Wendt, S, Wheelock, AM, Wiener, Z, Witte, L, Wolfram, J, Xagorari, A, Xander, P, Xu, J, Yan, X, Yanez-Mo, M, Yin, H, Yuana, Y, Zappulli, V, Zarubova, J, Zekas, V, Zhang, J-Y, Zhao, Z, Zheng, L, Zheutlin, AR, Zickler, AM, Zimmermann, P, Zivkovic, AM, Zocco, D, and Zuba-Surma, EK

Abstract

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Published
2018

45. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017

Author

Clayton, A, Buschmann, D, Byrd, JB, Carter, DRF, Cheng, L, Compton, C, Daaboul, G, Devitt, A, Manuel Falcon-Perez, J, Gardiner, C, Gustafson, D, Harrison, P, Helmbrecht, C, Hendrix, A, Hill, A, Hoffman, A, Jones, JC, Kalluri, R, Kang, JY, Kirchner, B, Lasser, C, Lawson, C, Lenassi, M, Levin, C, Llorente, A, Martens-Uzunova, ES, Moeller, A, Musante, L, Ochiya, T, Pink, RC, Tahara, H, Wauben, MHM, Webber, JP, Welsh, JA, Witwer, KW, Yin, H, Nieuwland, R, Clayton, A, Buschmann, D, Byrd, JB, Carter, DRF, Cheng, L, Compton, C, Daaboul, G, Devitt, A, Manuel Falcon-Perez, J, Gardiner, C, Gustafson, D, Harrison, P, Helmbrecht, C, Hendrix, A, Hill, A, Hoffman, A, Jones, JC, Kalluri, R, Kang, JY, Kirchner, B, Lasser, C, Lawson, C, Lenassi, M, Levin, C, Llorente, A, Martens-Uzunova, ES, Moeller, A, Musante, L, Ochiya, T, Pink, RC, Tahara, H, Wauben, MHM, Webber, JP, Welsh, JA, Witwer, KW, Yin, H, and Nieuwland, R

Abstract

This report summarises the presentations and activities of the ISEV Workshop on extracellular vesicle biomarkers held in Birmingham, UK during December 2017. Among the key messages was broad agreement about the importance of biospecimen science. Much greater attention needs to be paid towards the provenance of collected samples. The workshop also highlighted clear gaps in our knowledge about pre-analytical factors that alter extracellular vesicles (EVs). The future utility of certified standards for credentialing of instruments and software, to analyse EV and for tracking the influence of isolation steps on the structure and content of EVs were also discussed. Several example studies were presented, demonstrating the potential utility for EVs in disease diagnosis, prognosis, longitudinal serial testing and stratification of patients. The conclusion of the workshop was that more effort focused on pre-analytical issues and benchmarking of isolation methods is needed to strengthen collaborations and advance more effective biomarkers.

Published
2018

47. Concise Review: Developing Best-Practice Models for the Therapeutic Use of Extracellular Vesicles

Author

Reiner, AT, Witwer, KW, van Balkom, BWM, de Beer, J, Brodie, C, Corteling, RL, Gabrielsson, S, Gimona, M, Ibrahim, AG, de Kleijn, D, Lai, CP, Lotvall, J, del Portillo, HA, Reischl, IG, Riazifar, M, Salomon, C, Tahara, H, Toh, WS, Wauben, MHM, Yang, VK, Yang, YJ, Yeo, RWY, Yin, H, Giebel, B, Rohde, E, and Lim, SK

Subjects
Clinical trials, Clinical translation, Cellular therapy, Stem cells, Therapeutics, Extracellular vesicles, Exosomes, Microvesicles
Abstract

Growing interest in extracellular vesicles (EVs, including exosomes and microvesicles) as therapeutic entities, particularly in stem cell-related approaches, has underlined the need for standardization and coordination of development efforts. Members of the International Society for Extracellular Vesicles and the Society for Clinical Research and Translation of Extracellular Vesicles Singapore convened a Workshop on this topic to discuss the opportunities and challenges associated with development of EV-based therapeutics at the preclinical and clinical levels. This review outlines topic-specific action items that, if addressed, will enhance the development of best-practice models for EV therapies.

Published
2017

48. Obstacles and opportunities in the functionalanalysis of extracellular vesicle RNA – an ISEVposition paper

Author

Mateescu, B., Kowal, E. J. K., Bartel, S., Bhattacharyya, S. N., Buck, Amy H., Candia, P. de, Chow, F. W. N., Das, S., Driedonks, T. A. P., Fernández-Messina, L., Haderk, F., Hill, A. F., Jones, J. C., Lai, C. P., Lässer, C., Di Liegro, I., Magdalena J., Maas, S. L. N., Mittelbrunn, M., Mukherjee, K., Nawaz, M., Michiel Pegtel, D. M., Pfaffl, M. W., Schiffelers, R. M., Tahara, H., Théry, C., Tosar Rovira, Juan Pablo, Wauben, M. H. M., Witwer, K. W., Nolte-'tHoen, E. N. M., Buzás, E. I., and Tosar, Juan Pablo. Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares

Subjects
mRNA, Sorting, Quantification, RNA, RNA binding proteins, Extracellular vesicles, Function, Exosomes, Non-coding
Abstract

The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data.

Published
2017

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