Search

Background: Acinetobacter baumannii, are involved in hospital-acquired infections and are increasingly developing resistance to fluoroquinolones, such as ciprofloxacin. The most common method of fluoroquinolone resistance is alteration in genes that encode DNA gyrase (gyrA) and topoisomerase IV (parC).Methods: We sought to isolate fluoroquinolone-resistant A. baumannii and search for changes in gyrA (Ser83Leu) and parC (Ser80Leu) loci by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Results: We found that all 68 A. baumannii isolates that were part of this research were multidrug resistant and harbored gyrA and parC loci. Most isolates had ciprofloxacin minimal inhibitory concentrations of >128 µg/mL (50.8%). Mutations in gyrA were the most prevalent (47.45%), followed by parC mutations (33.9%) and combined mutations in both genes (23.7%).Conclusions: Single mutations in either gyrA (Ser83Leu) or parC (Ser80Leu) genes may be attributed to fluroquinolone resistance in A. baumannii. [ABSTRACT FROM AUTHOR]

Background: There has been an expansion in antibiotic resistance among Enterobacteriaceae over the previous ten years raising serious questions about global health. Our objectives were to isolate the carbapenem-resistant Gram-negative bacilli that cause blood stream infections and investigate the genetic relatedness between carbapenemase producing Klebsiella pneumoniae (K. pneumoniae) isolates. Methods: A total of 255 blood specimens were withdrawn into blood culture bottles and incubated, pathogenic microorganisms were identified, antibiotic susceptibility was done for Gram-negative isolates. Carbapenemase producers were screened for molecular detection of carbapenemase and extended spectrum ß-lactamase genes. Results: One hundred and thirty-three (133) pathogens were isolated, of these 77 Gram-negative isolates (including 32 K. pneumoniae, 18 E. coli, 12 Acinetobacter, 9 Pseudomonas and 6 Enterobacter cloacae). Out of them, 55 were carbapenem resistant by phenotypic method. 23/55 of them were harboring carbapenemase genes by PCR ERIC, results showed that Anesthesia intensive care unit (ICU) isolates showed >90% genetic similarity while isolates from Chest and Internal medicine ICUs showed <70% similarity. Conclusion: Increased awareness, persistent observation, and strict adherence to the recommendations for antibiotic stewardship should be used to limit spread of carbapenem resistant Gram-negative bacilli. [ABSTRACT FROM AUTHOR]

Copyright of Microbes & Infectious Diseases is the property of Microbes & Infectious Diseases and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)