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Reveal Salmonella 2.0 is an improved version of the original Reveal Salmonella lateral flow immunoassay and is applicable to the detection of Salmonella enterica serogroups A-E in a variety of food and environmental samples. A Performance Tested Method validation study was conducted to compare performance of the Reveal 2.0 method with that of the U.S. Department of Agriculture-Food Safety and Inspection Service or U.S. Food and Drug Administration/Bacteriological Analytical Manual reference culture methods for detection of Salmonella spp. in chicken carcass rinse, raw ground turkey, raw ground beef, hot dogs, raw shrimp, a ready-to-eat meal product, dry pet food, ice cream, spinach, cantaloupe, peanut butter, stainless steel surface, and sprout irrigation water. In a total of 17 trials performed internally and four trials performed in an independent laboratory, there were no statistically significant differences in performance of the Reveal 2.0 and reference culture procedures as determined by Chi-square analysis, with the exception of one trial with stainless steel surface and one trial with sprout irrigation water where there were significantly more positive results by the Reveal 2.0 method. Considering all data generated in testing food samples using enrichment procedures specifically designed for the Reveal method, overall sensitivity of the Reveal method relative to the reference culture methods was 99%. In testing environmental samples, sensitivity of the Reveal method relative to the reference culture method was 164%. For select foods, use of the Reveal test in conjunction with reference method enrichment resulted in overall sensitivity of 92%. There were no unconfirmed positive results on uninoculated control samples in any trials for specificity of 100%. In inclusivity testing, 102 different Salmonella serovars belonging to serogroups A-E were tested and 99 were consistently positive in the Reveal test. In exclusivity testing of 33 strains of non-salmonellae representing 14 genera, 32 were negative when tested with Reveal following nonselective enrichment, and the remaining strain was found to be substantially inhibited by the enrichment media used with the Reveal method. Results of ruggedness testing showed that the Reveal test produces accurate results even with substantial deviation in sample volume or device development time.

An experiment consisting of two fertilizer rates (69/10 and 46/20 kg N/P ha-1) and five nitrogen (N) application times (half at planting + half at tillering (control), half at planting + half at panicle initiation, one-third at planting + two-third at tillering, one-third at planting + two-third at panicle initiation and one-third at planting + one-third at tillering +one-third at panicle initiation) was conducted in factorial RCBD on the Vertisols of Fogera plain during the 2006 and 2007 cropping seasons. The objective of the experiment was to identify the best time of N application for maximum rain-fed lowland rice yield in the Fogera plain. Results showed significant difference in grain yield in response to the time of N application. The highest mean grain yield (4409 kg ha-1) was recorded when N was applied one-third at planting and two-third at the tillering stage of the crop. Nitrogen fertilizer rates and the interaction with its time of application showed non-significant differences for all parameters. The agronomic Nitrogen Use Efficiency (NUE) was found to be higher for one-third at planting and two-third at tillering nitrogen application compared to the other treatments. Hence, application of nitrogen one-third at planting and two-third at tillering stage of rice, irrespective of the fertilizer rate, is recommended for rice production in the Fogera plain.Keywords: split application, yield, NUE,.