74 results on '"Tabanelli V"'
Search Results
2. Non-hepatosplenic extramedullary manifestations in patients affected by Chronic myelomonocytic leukemia. Case report and meta-analysis of the published series.
- Author
-
Maraglino, A. M. E., Amato, V., Sammassimo, S., Gigli, F., Tabanelli, V., Pastano, R., Tarella, C., Giglio, F., and Derenzini, E.
- Subjects
CHRONIC leukemia ,GRAFT versus host disease - Abstract
HT
Patients affected by CMML with NEMMs Patients with AML transformation Patients, total number 7 All studies included in this analysis describe patients affected by CMML with histologically confirmed malignant NEMMs (defined by the presence of malignant CMML cells) occurring at any time during the disease course. NEMMs were documented in 47 patients (75%) within 12 months from initial CMML diagnosis and in 16 patients (25%) later in the disease course. The patient's cohort analyzed in this study includes 77 patients with malignant NEMMs from the published series and our case-report (78 patients in total). [Extracted from the article] - Published
- 2023
- Full Text
- View/download PDF
3. A 3-gene signature based on MYC, BCL-2 and NFKBIA improves risk stratification in diffuse large B-cell lymphoma
- Author
-
Derenzini E, Mazzara S, Melle F, Motta G, Fabbri M, Bruna R, Agostinelli C, Cesano A, Corsini CA, Chen N, Righi S, Sabattini E, Chiappella A, Calleri A, Fiori S, Tabanelli V, Cabras A, Pruneri G, Vitolo U, Gianni AM, Rambaldi A, Corradini P, Zinzani PL, Tarella C, Pileri S., and Derenzini E, Mazzara S, Melle F, Motta G, Fabbri M, Bruna R, Agostinelli C, Cesano A, Corsini CA, Chen N, Righi S, Sabattini E, Chiappella A, Calleri A, Fiori S, Tabanelli V, Cabras A, Pruneri G, Vitolo U, Gianni AM, Rambaldi A, Corradini P, Zinzani PL, Tarella C, Pileri S.
- Subjects
NFKBIA ,Diffuse Large B-cell Lymphoma ,BCL-2 ,MYC ,Gene expression profiling - Abstract
Recent randomized trials focused on gene expression-based determination of the cell of origin in diffuse large B-cell lymphoma could not show significant improvements by adding novel agents to standard chemoimmunotherapy. The aim of this study was the identification of a gene signature able to refine current prognostication algorithms and applicable to clinical practice. Here we used a targeted gene expression profiling panel combining the Lymph2Cx signature for cell of origin classification with additional targets including MYC, BCL-2 and NFKBIA, in 186 patients from 2 randomized trials (discovery cohort) (NCT00355199 and NCT00499018). Data were validated in 3 independent series (2 large public datasets and a real-life cohort). By integrating the cell of origin, MYC/BCL-2 double expressor status and NFKBIA expression, we defined a 3-gene signature combining MYC, BCL-2 and NFKBIA (MBN-signature), which outperformed the MYC/BCL-2 double expressor status in multivariate analysis, and allowed further risk stratification within the germinal center B-cell/unclassified subset. The high-risk (MBN Sig-high) subgroup identified the vast majority of double hit cases and a significant fraction of Activated B-Cell-derived diffuse large B-cell lymphomas. These results were validated in 3 independent series including a cohort from the REMoDL-B trial, where, in an exploratory ad hoc analysis, the addition of bortezomib in the MBN Sig-high subgroup provided a progression free survival advantage compared with standard chemoimmunotherapy. These data indicate that a simple 3-gene signature based on MYC, BCL-2 and NFKBIA could refine the prognostic stratification in diffuse large B-cell lymphoma, and might be the basis for future precision-therapy approaches.
- Published
- 2021
4. Corrigendum: Dissection of DLBCL microenvironment provides a gene expression-based predictor of survival applicable to formalin-fixed paraffin-embedded tissue (Annals of Oncology (2018) 29 (2363-2370) DOI: 10.1093/annonc/mdy450)
- Author
-
Ciavarella S., Vegliante M. C., Fabbri M., De Summa S., Melle F., Motta G., De Iuliis V., Opinto G., Enjuanes A., Rega S., Gulino A., Agostinelli C., Scattone A., Tommasi S., Mangia A., Mele F., Simone G., Zito A. F., Ingravallo G., Vitolo U., Chiappella A., Tarella C., Gianni A. M., Rambaldi A., Zinzani P. L., Casadei B., Derenzini E., Loseto G., Pileri A., Tabanelli V., Fiori S., Rivas-Delgado A., Lopez-Guillermo A., Venesio T., Sapino A., Campo E., Tripodo C., Guarini A., Pileri S. A., Ciavarella S., Vegliante M.C., Fabbri M., De Summa S., Melle F., Motta G., De Iuliis V., Opinto G., Enjuanes A., Rega S., Gulino A., Agostinelli C., Scattone A., Tommasi S., Mangia A., Mele F., Simone G., Zito A.F., Ingravallo G., Vitolo U., Chiappella A., Tarella C., Gianni A.M., Rambaldi A., Zinzani P.L., Casadei B., Derenzini E., Loseto G., Pileri A., Tabanelli V., Fiori S., Rivas-Delgado A., Lopez-Guillermo A., Venesio T., Sapino A., Campo E., Tripodo C., Guarini A., and Pileri S.A.
- Subjects
DLBCL microenvironment, gene expression DLBCL - Abstract
The affiliations of authors T. Venesio and A. Sapino were originally incorrect. These have now been corrected as per the author listing above.
- Published
- 2019
5. Dissection of DLBCL microenvironment provides a gene expression-based predictor of survival applicable to formalin-fixed paraffin-embedded tissue
- Author
-
Ciavarella, S., primary, Vegliante, M.C., additional, Fabbri, M., additional, De Summa, S., additional, Melle, F., additional, Motta, G., additional, De Iuliis, V., additional, Opinto, G., additional, Enjuanes, A., additional, Rega, S., additional, Gulino, A., additional, Agostinelli, C., additional, Scattone, A., additional, Tommasi, S., additional, Mangia, A., additional, Mele, F., additional, Simone, G., additional, Zito, A.F., additional, Ingravallo, G., additional, Vitolo, U., additional, Chiappella, A., additional, Tarella, C., additional, Gianni, A.M., additional, Rambaldi, A., additional, Zinzani, P.L., additional, Casadei, B., additional, Derenzini, E., additional, Loseto, G., additional, Pileri, A., additional, Tabanelli, V., additional, Fiori, S., additional, Rivas-Delgado, A., additional, López-Guillermo, A., additional, Venesio, T., additional, Sapino, A., additional, Campo, E., additional, Tripodo, C., additional, Guarini, A., additional, and Pileri, S.A., additional
- Published
- 2019
- Full Text
- View/download PDF
6. INTEGRATING TUMOR- AND MICROENVIRONMENT-REFLECTING GENES IN A UNIQUE AND ROUTINE-APPLICABLE ASSAY FOR ACCURATE RISK PREDICTION IN DLBCL
- Author
-
Pileri, S.A., primary, Mazzara, S., additional, Derenzini, E., additional, Melle, F., additional, Motta, G., additional, Tabanelli, V., additional, Vitolo, U., additional, Chiappella, A., additional, Fabbri, M., additional, Agostinelli, C., additional, Tarella, C., additional, Rambaldi, A., additional, Guarini, A., additional, Opinto, G., additional, Vegliante, M.C., additional, De Summa, S., additional, and Ciavarella, S., additional
- Published
- 2019
- Full Text
- View/download PDF
7. PO-272 Leukemia-associated NPM mutations promote quiescence of hematopoietic stem cells and prevent their functional exhaustion upon oncogene-induced hyper-proliferation
- Author
-
Merlo, M.E. Boggio, primary, Mallardo, M., additional, De Conti, G., additional, Tabanelli, V., additional, Calleri, A., additional, Pileri, S., additional, Pelicci, P.G., additional, and Colombo, E., additional
- Published
- 2018
- Full Text
- View/download PDF
8. Pathogenetic and diagnostic significance of microRNA deregulation in peripheral T-cell lymphoma not otherwise specified
- Author
-
Laginestra, M A, primary, Piccaluga, P P, additional, Fuligni, F, additional, Rossi, M, additional, Agostinelli, C, additional, Righi, S, additional, Sapienza, M R, additional, Motta, G, additional, Gazzola, A, additional, Mannu, C, additional, Sabattini, E, additional, Bacci, F, additional, Tabanelli, V, additional, Sacchetti, C A S, additional, Barrese, T Z, additional, Etebari, M, additional, Melle, F, additional, Clò, A, additional, Gibellini, D, additional, Tripodo, C, additional, Inghirami, G, additional, Croce, C M, additional, and Pileri, S A, additional
- Published
- 2014
- Full Text
- View/download PDF
9. CD30 expression in peripheral T-cell lymphomas
- Author
-
Sabattini, E., primary, Pizzi, M., additional, Tabanelli, V., additional, Baldin, P., additional, Sacchetti, C. S., additional, Agostinelli, C., additional, Zinzani, P. L., additional, and Pileri, S. A., additional
- Published
- 2013
- Full Text
- View/download PDF
10. Systemic Epstein-Barr-virus-positive T cell lymphoproliferative childhood disease in a 22-year-old Caucasian man: A case report and review of the literature
- Author
-
Meloni Giovanna, Sista Maria, Righi Simona, Mannu Claudia, Capria Saveria, Bacci Francesco, Gazzola Anna, Sabattini Elena, Agostinelli Claudio, Tabanelli Valentina, Pileri Stefano A, and Piccaluga Pier
- Subjects
Medicine - Abstract
Abstract Introduction Systemic Epstein-Barr-virus-positive T cell lymphoproliferative disease of childhood is an extremely rare disorder, characterized by clonal proliferation of Epstein-Barr-virus-infected T cells with an activated cytotoxic phenotype. The disease is more frequent in Asia and South America, with only few cases reported in Western countries. A prompt diagnosis, though often difficult, is a necessity due to the very aggressive clinical course of the disease. Case presentation We report the clinicopathological features of fulminant T cell lymphoproliferative disease that arose in the setting of acute primary Epstein-Barr virus infection. Our patient, a 23-year-old man, presented to our facility with persisting fever, hepatosplenomegaly and severe pancytopenia. On bone marrow biopsy, an abundant lymphoid infiltrate was observed. Immunophenotypic and molecular studies revealed that the atypical lymphoid cells displayed a CD8+, Epstein-Barr-encoded-RNA-positive T cell phenotype with clonal rearrangement of the T cell receptor genes, the final diagnosis being systemic Epstein-Barr-virus-positive T cell lymphoproliferative disease. On reviewing the literature we found only 14 similar cases, all presenting with very aggressive clinical courses and requiring extensive phenotyping and molecular techniques for final diagnosis. Conclusion Though extremely rare, this disease can occur in Europe, and a comprehensive diagnostic approach is thus recommended in all case of Epstein-Barr-virus-positive lymphoproliferative disorders. Unfortunately, at present no specific treatment is available; however, prompt administration of anti- Epstein-Barr virus treatment and rapid attempts to control the hemophagocytic syndrome are indicated.
- Published
- 2011
- Full Text
- View/download PDF
11. Venetoclax Shows Low Therapeutic Activity in BCL2-Positive Relapsed/Refractory Peripheral T-Cell Lymphoma: A Phase 2 Study of the Fondazione Italiana Linfomi
- Author
-
Laura Ballotta, Pier Luigi Zinzani, Stefano Pileri, Riccardo Bruna, Monica Tani, Beatrice Casadei, Valentina Tabanelli, Stefano Volpetti, Stefano Luminari, Paolo Corradini, Elisa Lucchini, Maria Chiara Tisi, Michele Merli, Alessandro Re, Marzia Varettoni, Emanuela Anna Pesce, Francesco Zaja, Ballotta, L., Zinzani, P. L., Pileri, S., Bruna, R., Tani, M., Casadei, B., Tabanelli, V., Volpetti, S., Luminari, S., Corradini, P., Lucchini, E., Tisi, M. C., Merli, M., Re, A., Varettoni, M., Pesce, E. A., Zaja, F., Ballotta L., Zinzani P.L., Pileri S., Bruna R., Tani M., Casadei B., Tabanelli V., Volpetti S., Luminari S., Corradini P., Lucchini E., Tisi M.C., Merli M., Re A., Varettoni M., Pesce E.A., and Zaja F.
- Subjects
BCL2 inhibition ,BCL2 protein ,peripheral T-cell lymphoma ,relapsed/refractory ,venetoclax ,Cancer Research ,Oncology ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Clinical Trial ,RC254-282 - Abstract
Patients with relapsed/refractory (R/R) peripheral T-cell lymphoma (PTCL) have a poor prognosis, with an expected survival of less than 1 year using standard salvage therapies. Recent advances in our understanding of the biology of PTCL have led to identifying B-Cell Lymphoma 2 (BCL2) protein as a potential therapeutic target. BLC2 inhibitor venetoclax was investigated in a prospective phase II trial in patients with BCL2-positive R/R PTCL after at least one previous standard line of treatment (NCT03552692). Venetoclax given alone at a dosage of 800 mg/day resulted in one complete response (CR) and two stable diseases (SDs) among 17 enrolled patients. The majority of patients (88.2%) interrupted the treatment due to disease progression. No relationship with BCL2 expression was documented. At a median follow-up of 8 months, two patients are currently still on treatment (one CR and one SD). No case of tumor lysis syndrome was registered. Therefore, venetoclax monotherapy shows activity in a minority of patients whose biological characteristics have not yet been identified.Clinical Trial Registrationwww.clinicaltrials.gov (NCT03552692, EudraCT number 2017-004630-29).
- Published
- 2021
12. Blastic plasmacytoid dendritic cell neoplasm: genomics mark epigenetic dysregulation as a primary therapeutic target
- Author
-
Fabio Facchetti, Emilio Berti, Claudio Tripodo, Maryam Etebari, Giovanna Motta, Nicola Pimpinelli, Fabio Fuligni, Stefano Pileri, Gaetano Ivan Dellino, Alessandro Pileri, Claudio Agostinelli, Valentina Indio, Marco Paulli, Stefania Orecchioni, Stefano Amente, Federica Melle, Giovanna Talarico, Maria Antonella Laginestra, Giuseppe Tarantino, Francesco Bertolini, Maura Rossi, Elena Sabattini, Francesco Abate, Raul Rabadan, Valentina Tabanelli, Lorenzo Cerroni, Francesco Gavino Brundu, Mauro Truni, Rossana Piccioni, Maria Rosaria Sapienza, Brunangelo Falini, Sapienza M.R., Abate F., Melle F., Orecchioni S., Fuligni F., Etebari M., Tabanelli V., Laginestra M.A., Pileri A., Motta G., Rossi M., Agostinelli C., Sabattini E., Pimpinelli N., Truni M., Falini B., Cerroni L., Talarico G., Piccioni R., Amente S., Indio V., Tarantino G., Brundu F., Paulli M., Berti E., Facchetti F., Dellino G.I., Bertolini F., Tripodo C., Rabadan R., Pileri S.A., Sapienza, M. R., Abate, F., Melle, F., Orecchioni, S., Fuligni, F., Etebari, M., Tabanelli, V., Laginestra, M. A., Pileri, A., Motta, G., Rossi, M., Agostinelli, C., Sabattini, E., Pimpinelli, N., Truni, M., Falini, B., Cerroni, L., Talarico, G., Piccioni, R., Amente, S., Indio, V., Tarantino, G., Brundu, F., Paulli, M., Berti, E., Facchetti, F., Dellino, G. I., Bertolini, F., Tripodo, C., Rabadan, R., Pileri, S. A., Sapienza, Maria Rosaria, Abate, Francesco, Melle, Federica, Orecchioni, Stefania, Fuligni, Fabio, Etebari, Maryam, Tabanelli, Valentina, Laginestra, Maria Antonella, Pileri, Alessandro, Motta, Giovanna, Rossi, Maura, Agostinelli, Claudio, Sabattini, Elena, Pimpinelli, Nicola, Truni, Mauro, Falini, Brunangelo, Cerroni, Lorenzo, Talarico, Giovanna, Piccioni, Rossana, Amente, Stefano, Indio, Valentina, Tarantino, Giuseppe, Brundu, Francesco, Paulli, Marco, Berti, Emilio, Facchetti, Fabio, Dellino, Gaetano Ivan, Bertolini, Francesco, Tripodo, Claudio, Rabadan, Raul, and Pileri, Stefano A
- Subjects
Acute Myeloid Leukemia ,Blastic plasmacytoid dendritic cell neoplasm, epigenetic mutations ,Skin Neoplasms ,Azacitidine ,Decitabine ,Plasmacytoid dendritic cell ,Gene mutation ,Biology ,BPDCN ,Article ,Epigenesis, Genetic ,03 medical and health sciences ,0302 clinical medicine ,Histone methylation ,5-Azacytidine ,WES ,medicine ,Humans ,Epigenetics ,Exome sequencing ,Regulation of gene expression ,Myeloproliferative Disorders ,Dendritic Cells ,Genomics ,Hematology ,5 -Azacytidine ,Myeloid Neoplasms ,Cancer research ,030215 immunology ,medicine.drug - Abstract
Blastic Plasmacytoid Dendritic Cell Neoplasm is a rare and aggressive hematological malignancy currently lacking an effective therapy. To possibly identify genetic alterations useful for a new treatment design, we analyzed by whole-exome sequencing fourteen Blastic Plasmacytoid Dendritic Cell Neoplasm patients and the patient-derived CAL-1 cell line. The functional enrichment analysis of mutational data reported the epigenetic regulatory program as the most significantly undermined (P
- Published
- 2018
13. Newly-Discovered Neural Features Expand the Pathobiological Knowledge of Blastic Plasmacytoid Dendritic Cell Neoplasm
- Author
-
Lorenzo Cerroni, Federica Melle, Marcello Del Corvo, Marco Paulli, Emilio Berti, Jessica Consiglio, Gaetano Ivan Dellino, Giovanna Motta, Stefano Pileri, Carlo M. Croce, Vincenzo Mazzara, Stefano Fiori, Fabio Fuligni, Giuseppe Benvenuto, Alessandro Pileri, Fabio Facchetti, Claudio Tripodo, Daniele Fanoni, Maria Rosaria Sapienza, Manuela Ferracin, Elena Sabattini, Valentina Tabanelli, Saveria Mazzara, Beatrice Belmonte, Sapienza M.R., Benvenuto G., Ferracin M., Mazzara S., Fuligni F., Tripodo C., Belmonte B., Fanoni D., Melle F., Motta G., Tabanelli V., Consiglio J., Mazzara V., Corvo M.D., Fiori S., Pileri A., Dellino G.I., Cerroni L., Facchetti F., Berti E., Sabattini E., Paulli M., Croce C.M., and Pileri S.A.
- Subjects
Cancer Research ,Neurogenesis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,MicroRNA Expression Profile ,sequencing ,Biology ,Settore MED/08 - Anatomia Patologica ,BPDCN, MiRNA, Network, Neurogenesis, Sequencing ,BPDCN ,Article ,Chromatin ,Gene expression profiling ,MiRNA ,Network ,Sequencing ,neurogenesis ,Oncology ,Downregulation and upregulation ,microRNA ,network ,Cancer research ,Immunohistochemistry ,Settore MED/05 - Patologia Clinica ,Neurogenesi ,RC254-282 ,Progenitor ,miRNA - Abstract
Simple Summary For the first time, neuronal features are described in blastic plasmacytoid dendritic cell neoplasm (BPDCN) by a complex array of molecular techniques, including microRNA and gene expression profiling, RNA and Chromatin immunoprecipitation sequencing, and immunohistochemistry. The discovery of unexpected neural features in BPDCN may change our vision of this disease, leading to the designing of a new BPDCN cell model and to re-thinking the relations occurring between BPDCN and nervous system. The observed findings contribute to explaining the extreme tumor aggressiveness and also to propose novel therapeutic targets. In view of this, the identification, in this work of new potential neural metastatic inducers might open the way to therapeutic approaches for BPDCN patients based on the use of anti-neurogenic agents. Abstract Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and highly aggressive hematologic malignancy originating from plasmacytoid dendritic cells (pDCs). The microRNA expression profile of BPDCN was compared to that of normal pDCs and the impact of miRNA dysregulation on the BPDCN transcriptional program was assessed. MiRNA and gene expression profiling data were integrated to obtain the BPDCN miRNA-regulatory network. The biological process mainly dysregulated by this network was predicted to be neurogenesis, a phenomenon raising growing interest in solid tumors. Neurogenesis was explored in BPDCN by querying different molecular sources (RNA sequencing, Chromatin immunoprecipitation-sequencing, and immunohistochemistry). It was shown that BPDCN cells upregulated neural mitogen genes possibly critical for tumor dissemination, expressed neuronal progenitor markers involved in cell migration, exchanged acetylcholine neurotransmitter, and overexpressed multiple neural receptors that may stimulate tumor proliferation, migration and cross-talk with the nervous system. Most neural genes upregulated in BPDCN are currently investigated as therapeutic targets.
- Published
- 2021
14. Predictive and Prognostic Molecular Factors in Diffuse Large B-Cell Lymphomas
- Author
-
Claudio Tripodo, S. Ciavarella, Stefano Pileri, Federica Melle, Maria Carmela Vegliante, Stefano Fiori, Saveria Mazzara, Giovanna Motta, Valentina Tabanelli, Enrico Derenzini, Pileri S.A., Tripodo C., Melle F., Motta G., Tabanelli V., Fiori S., Vegliante M.C., Mazzara S., Ciavarella S., and Derenzini E.
- Subjects
0301 basic medicine ,Oncology ,medicine.medical_specialty ,diagnosis ,diffuse large B-cell lymphoma ,Review ,Settore MED/08 - Anatomia Patologica ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Daily practice ,medicine ,Tumor Microenvironment ,Humans ,lcsh:QH301-705.5 ,B cell ,therapy ,business.industry ,Gene Expression Profiling ,Not Otherwise Specified ,High-Throughput Nucleotide Sequencing ,General Medicine ,medicine.disease ,Microarray Analysis ,Prognosis ,Lymphoma ,Gene expression profiling ,diagnosi ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Biology (General) ,Lymphoid malignancy ,classification ,030220 oncology & carcinogenesis ,next-generation sequencing ,Lymphoma, Large B-Cell, Diffuse ,business ,Diffuse large B-cell lymphoma ,prognosi - Abstract
Diffuse large B-cell lymphoma (DLBCL) is the commonest form of lymphoid malignancy, with a prevalence of about 40% worldwide. Its classification encompasses a common form, also termed as “not otherwise specified” (NOS), and a series of variants, which are rare and at least in part related to viral agents. Over the last two decades, DLBCL-NOS, which accounts for more than 80% of the neoplasms included in the DLBCL chapter, has been the object of an increasing number of molecular studies which have led to the identification of prognostic/predictive factors that are increasingly entering daily practice. In this review, the main achievements obtained by gene expression profiling (with respect to both neoplastic cells and the microenvironment) and next-generation sequencing will be discussed and compared. Only the amalgamation of molecular attributes will lead to the achievement of the long-term goal of using tailored therapies and possibly chemotherapy-free protocols capable of curing most (if not all) patients with minimal or no toxic effects.
- Published
- 2021
15. MicroRNA profiling of blastic plasmacytoid dendritic cell neoplasm and myeloid sarcoma
- Author
-
Maria Antonella Laginestra, Luciano Cascione, Claudio Agostinelli, Elena Sabattini, Marco Paulli, Saveria Mazzara, Emilio Berti, Carlo M. Croce, Stefano Pileri, Giovanna Motta, Lorenzo Cerroni, Alessandro Laganà, Fabio Facchetti, Federica Melle, Alessandro Pileri, Valentina Tabanelli, Maria Rosaria Sapienza, Valentina Indio, Fabio Fuligni, Manuela Ferracin, Sapienza M.R., Fuligni F., Melle F., Tabanelli V., Indio V., Laginestra M.A., Motta G., Mazzara S., Cerroni L., Pileri A., Facchetti F., Paulli M., Cascione L., Lagana A., Berti E., Ferracin M., Agostinelli C., Sabattini E., Croce C.M., and Pileri S.A.
- Subjects
Cancer Research ,Biology ,BPDCN ,microRNA ,Myeloid sarcoma ,medicine ,Humans ,Sarcoma, Myeloid ,Survival analysis ,miRNA ,discriminant analysis ,miRNAs ,MS ,Hematology ,General Medicine ,Blastic plasmacytoid dendritic cell neoplasm ,Dendritic Cells ,medicine.disease ,Survival Analysis ,MicroRNAs ,Oncology ,Hematologic Neoplasms ,Cancer research ,Microrna profiling ,Blast Crisis ,discriminant analysi ,Plasmacytoma - Abstract
not present
- Published
- 2020
16. Recurrent PDL1 expression and PDL1 (CD274) copy number alterations in breast implant-associated anaplastic large cell lymphomas
- Author
-
Stefano Pileri, Anna Rotili, Claudio Agostinelli, Chiara Corsini, Valentina Tabanelli, Federica Melle, Arianna Di Napoli, Angelica Calleri, Stefania Orecchioni, Giovanna Motta, Stefano Fiori, Tabanelli V., Corsini C., Fiori S., Agostinelli C., Calleri A., Orecchioni S., Melle F., Motta G., Rotili A., Di Napoli A., and Pileri S.A.
- Subjects
0301 basic medicine ,Adult ,STAT3 Transcription Factor ,Anaplastic Lymphoma ,DNA Copy Number Variations ,Breast Implants ,Breast Neoplasms ,In situ hybridization ,B7-H1 Antigen ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,hemic and lymphatic diseases ,Breast implant ,PDL1 ,Gene duplication ,medicine ,Biomarkers, Tumor ,Humans ,Phosphorylation ,STAT3 ,Anaplastic large-cell lymphoma ,Aged ,Polysomy ,anaplastic lymphoma ,breast implant ,copy gain ,pdl1 ,polysomy ,biology ,Copy gain ,JAK-STAT signaling pathway ,Middle Aged ,medicine.disease ,Immunohistochemistry ,030104 developmental biology ,030220 oncology & carcinogenesis ,Anaplastic lymphoma ,biology.protein ,Cancer research ,Lymphoma, Large-Cell, Anaplastic ,Female - Abstract
Breast implant-associated anaplastic large cell lymphoma (BI-ALCL) is a variant of anaplastic large cell lymphoma arising within seroma effusion associated with breast implants. BI-ALCL is a rare disease, recently recognized as a new provisional entity by the 2017 revised World Health Organization classification. All BI-ALCLs tested so far showed a "triple-negative" genetic profile-negative for ALK, DUSP22, and TP63 rearrangements-and were characterized by mutational and gene expression profiles consistent with aberrant activation of the JAK/STAT pathway. The active form of STAT3 (pSTAT3) is constantly expressed in BI-ALCLs and may favor tumor immune escape by triggering the transcription of PDL1 (CD274), a gene encoding the immune-checkpoint molecule programmed cell death ligand 1 (PDL1); immunohistochemical positivity for PDL1 has been recently described in 3 BI-ALCL cases, and one of them also harbored PDL1 gene amplification. We evaluated PDL1 and pSTAT expression by immunohistochemistry and PDL1 copy number alterations (CNAs) at chromosome 9p24.1 by fluorescent in situ hybridization in a cohort of 9 BI-ALCL cases; we also investigated the presence of tumor-infiltrating programmed cell death 1 (PD1)+ T cells (tumor-infiltrating lymphocytes, or TILs) and PDL1+ tumor-associated macrophages (TAMs) in BI-ALCL microenvironment. Tumor cells expressed PDL1 in 5 (56%) of 9 cases and harbored PDL1 CNAs in 3 (33%) of 9 cases; immunohistochemistry for pSTAT3 was positive in all 6 cases tested (100%), indicative of active JAK/STAT signaling. We observed PDL1 CNAs only among PDL1-positive cases, whereas PD1+ TILs and PDL1+ TAMs were present at variable levels in both PDL1-positive and PDL1-negative BI-ALCLs. We report frequent PDL1 expression and recurrent PDL1 CNAs in BI-ALCLs: our data suggest that 9p24.1 alterations represent a common mechanism of PDL1 overexpression in this disease, likely acting in synergy with constitutive pSTAT3 signaling. In PDL1-positive cases without chromosomal aberration, PDL1 expression may be induced by JAK/STAT signaling alone and/or others alternative pathways. BI-ALCL microenvironment hosts variable amounts of PD1+ TILs and PDL1+ TAMs, suggesting the presence of an active PD1/PDL1 axis. These findings may be of therapeutic value in advanced-stage patients who may benefit from a PD1/PDL1 blocking treatment.
- Published
- 2019
17. Pathogenetic and diagnostic significance of microRNA deregulation in peripheral T-cell lymphoma not otherwise specified
- Author
-
Claudio Agostinelli, Federica Melle, Giorgio Inghirami, Tabanelli, Anna Gazzola, Martina Rossi, G Motta, Alberto Clò, Simona Righi, Fabio Fuligni, Tomas Barrese, Pier Paolo Piccaluga, Claudio Tripodo, Carlo Alberto Sagramoso Sacchetti, Maria Antonella Laginestra, Davide Gibellini, Stefano Pileri, Claudia Mannu, Elena Sabattini, Francesco Bacci, Carlo M. Croce, Maryam Etebari, Maria Rosaria Sapienza, Laginestra, M., Piccaluga, P., Fuligni, F., Rossi, M., Agostinelli, C., Righi, S., Sapienza, M., Motta, G., Gazzola, A., Mannu, C., Sabattini, E., Bacci, E., Tabanelli, V., Sacchetti, C., Barrese, T., Etebari, M., Melle, F., Clò, A., Gibellini, D., Tripodo, C., Inghirami, G., Croce, C., Pileri, S., Laginestra, M.A, Piccaluga, P.P., Sapienza, M.R., Sagramoso Sacchetti, C.A., Barrese, T.Z., Croce, C.M., and Pileri, S.A.
- Subjects
Female ,Gene Expression Profiling ,Humans ,Lymphoma, T-Cell, Peripheral ,Male ,MicroRNAs ,Oligonucleotide Array Sequence Analysis ,RNA, Neoplasm ,Gene Expression Regulation, Neoplastic ,Oncology ,Hematology ,Medicine (all) ,medicine.medical_specialty ,Pathology ,Peripheral T-cell lymphoma not otherwise specified ,Biology ,hemic and lymphatic diseases ,Internal medicine ,microRNA ,medicine ,Regulation of gene expression ,PTCLs/NOS ,GEP ,Oligonucleotide Array Sequence Analysi ,Not Otherwise Specified ,MicroRNA ,medicine.disease ,Lymphoma ,Gene expression profiling ,Original Article ,CD8 ,Human - Abstract
Peripheral T-cell lymphomas not otherwise specified (PTCLs/NOS) are rare and aggressive tumours whose molecular pathogenesis and diagnosis are still challenging. The microRNA (miRNA) profile of 23 PTCLs/NOS was generated and compared with that of normal T-lymphocytes (CD4+, CD8+, naive, activated). The differentially expressed miRNA signature was compared with the gene expression profile (GEP) of the same neoplasms. The obtained gene patterns were tested in an independent cohort of PTCLs/NOS. The miRNA profile of PTCLs/NOS then was compared with that of 10 angioimmunoblastic T-cell lymphomas (AITLs), 6 anaplastic large-cell lymphomas (ALCLs)/ALK+ and 6 ALCLs/ALK - . Differentially expressed miRNAs were validated in an independent set of 20 PTCLs/NOS, 20 AITLs, 19 ALCLs/ALK - and 15 ALCLs/ALK+. Two hundred and thirty-six miRNAs were found to differentiate PTCLs/NOS from activated T-lymphocytes. To assess which miRNAs impacted on GEP, a multistep analysis was performed, which identified all miRNAs inversely correlated to different potential target genes. One of the most discriminant miRNAs was selected and its expression was found to affect the global GEP of the tumours. Moreover, two sets of miRNAs were identified distinguishing PTCL/NOS from AITL and ALCL/ALK - , respectively. The diagnostic accuracy of this tool was very high (83.54%) and its prognostic value validated.
- Published
- 2014
18. Systemic Epstein-Barr-virus-positive T cell lymphoproliferative childhood disease in a 22-year-old Caucasian man: A case report and review of the literature
- Author
-
Claudio Agostinelli, Stefano Pileri, Valentina Tabanelli, Simona Righi, Claudia Mannu, Maria Teresa Sista, Saveria Capria, Anna Gazzola, Pier Paolo Piccaluga, Elena Sabattini, Giovanna Meloni, Francesco Bacci, Tabanelli V., Agostinelli C., Sabattini E., Gazzola A., Bacci F., Capria S., Mannu C., Righi S., Sista M.T., Meloni G., Pileri S.A., and Piccaluga P.P.
- Subjects
Epstein-Barr-viru ,Pathology ,medicine.medical_specialty ,PTCL ,Fulminant ,T cell ,Hepatosplenomegaly ,Lymphoproliferative disorders ,lcsh:Medicine ,Case Report ,Disease ,EBV ,hemic and lymphatic diseases ,medicine ,Cytotoxic T cell ,Medicine(all) ,business.industry ,lcsh:R ,Peripheral T-cell lymphoma ,General Medicine ,medicine.disease ,Pancytopenia ,medicine.anatomical_structure ,medicine.symptom ,business ,CD8 - Abstract
Introduction Systemic Epstein-Barr-virus-positive T cell lymphoproliferative disease of childhood is an extremely rare disorder, characterized by clonal proliferation of Epstein-Barr-virus-infected T cells with an activated cytotoxic phenotype. The disease is more frequent in Asia and South America, with only few cases reported in Western countries. A prompt diagnosis, though often difficult, is a necessity due to the very aggressive clinical course of the disease. Case presentation We report the clinicopathological features of fulminant T cell lymphoproliferative disease that arose in the setting of acute primary Epstein-Barr virus infection. Our patient, a 23-year-old man, presented to our facility with persisting fever, hepatosplenomegaly and severe pancytopenia. On bone marrow biopsy, an abundant lymphoid infiltrate was observed. Immunophenotypic and molecular studies revealed that the atypical lymphoid cells displayed a CD8+, Epstein-Barr-encoded-RNA-positive T cell phenotype with clonal rearrangement of the T cell receptor genes, the final diagnosis being systemic Epstein-Barr-virus-positive T cell lymphoproliferative disease. On reviewing the literature we found only 14 similar cases, all presenting with very aggressive clinical courses and requiring extensive phenotyping and molecular techniques for final diagnosis. Conclusion Though extremely rare, this disease can occur in Europe, and a comprehensive diagnostic approach is thus recommended in all case of Epstein-Barr-virus-positive lymphoproliferative disorders. Unfortunately, at present no specific treatment is available; however, prompt administration of anti- Epstein-Barr virus treatment and rapid attempts to control the hemophagocytic syndrome are indicated.
- Published
- 2010
19. Atezolizumab, venetoclax, and obinutuzumab combination in Richter transformation diffuse large B-cell lymphoma (MOLTO): a multicentre, single-arm, phase 2 trial.
- Author
-
Tedeschi A, Frustaci AM, Condoluci A, Coscia M, Chiarle R, Zinzani PL, Motta M, Gaidano G, Quaresmini G, Scarfò L, Catania G, Deodato M, Jones R, Tabanelli V, Griggio V, Stüssi G, Calleri A, Pini K, Cairoli R, Zenz T, Signori A, Zucca E, Rossi D, and Montillo M
- Subjects
- Humans, Female, Male, Aged, Middle Aged, Prospective Studies, Aged, 80 and over, Adult, Leukemia, Lymphocytic, Chronic, B-Cell drug therapy, Leukemia, Lymphocytic, Chronic, B-Cell pathology, Leukemia, Lymphocytic, Chronic, B-Cell mortality, Lymphoma, Large B-Cell, Diffuse drug therapy, Lymphoma, Large B-Cell, Diffuse pathology, Lymphoma, Large B-Cell, Diffuse mortality, Antibodies, Monoclonal, Humanized administration & dosage, Antibodies, Monoclonal, Humanized adverse effects, Antibodies, Monoclonal, Humanized therapeutic use, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Antineoplastic Combined Chemotherapy Protocols adverse effects, Antineoplastic Combined Chemotherapy Protocols administration & dosage, Sulfonamides administration & dosage, Sulfonamides adverse effects, Bridged Bicyclo Compounds, Heterocyclic administration & dosage, Bridged Bicyclo Compounds, Heterocyclic adverse effects, Bridged Bicyclo Compounds, Heterocyclic therapeutic use
- Abstract
Background: The diffuse large B-cell lymphoma (DLBCL) variant of Richter transformation (DLBCL-RT) is typically chemoresistant with poor prognosis. Aiming to explore a chemotherapy-free treatment combination that triggers anti-tumour immune responses, we conducted a phase 2 study of atezolizumab (a PD-L1 inhibitor) in combination with venetoclax and obinutuzumab in patients with DLBCL-RT., Methods: This was a prospective, open-label, multicentre, single-arm, investigator-initiated, phase 2 study in 15 hospitals in Italy and Switzerland. Eligible patients had a confirmed diagnosis of chronic lymphocytic leukaemia or small lymphocytic lymphoma as per the International Workshop on Chronic Lymphocytic Leukemia (IWCLL) 2008 criteria with biopsy-proven transformation to DLBCL; had not previously received treatment for DLBCL-RT, although they could have received chronic lymphocytic leukaemia therapies; were aged 18 years or older; and had an Eastern Cooperative Oncology Group (ECOG) performance status of 0-2. No previous treatment with any of the drugs in the triplet combination was allowed. Patients received 35 cycles of 21 days of intravenous obinutuzumab (100 mg on day 1, 900 mg on day 2, 1000 mg on day 8 and day 15 of cycle 1; 1000 mg on day 1 of cycles 2-8) and intravenous atezolizumab (1200 mg on day 2 of cycle 1 and 1200 mg on day 1 of cycles 2-18), and continuous oral venetoclax (ramp-up from 20 mg/day on day 15 of cycle 1 according to chronic lymphocytic leukaemia schedule, then 400 mg/day from day 1 of cycle 3 to day 21 of cycle 35). The primary endpoint was overall response rate at day 21 of cycle 6 in the intention-to-treat population. We considered an overall response rate of 67% or more to be clinically active, rejecting the null hypothesis of a response of 40% or less. The study is registered with ClinicalTrials.gov, NCT04082897, and has been completed., Findings: Between Oct 9, 2019, and Oct 19, 2022, 28 patients were enrolled (12 [43%] male patients and 16 [57%] female patients). Median follow-up was 16·8 months (IQR 7·8-32·0). At cycle 6, 19 of 28 patients showed a response, yielding an overall response rate of 67·9% (95% CI 47·6-84·1). Treatment-emergent adverse events that were grade 3 or worse were reported in 17 (61%; 95% CI 40·6-78·5) of 28 patients, with neutropenia being the most frequent (11 [39%; 21·5-59·4] of 28 patients). Serious treatment-emergent adverse events were reported in eight (29%; 14·2-48·7) patients, which were most commonly infections (five [18%; 6·1-36·9] of 28 patients). There were two (7%) deaths attributable to adverse events during the study: one from sepsis and one from fungal pneumonia, which were not considered as directly treatment-related by the investigators. Six (21·4%) patients had immune-related adverse events, none of which led to discontinuation. No tumour lysis syndrome was observed., Interpretation: The atezolizumab, venetoclax, and obinutuzumab triplet combination was shown to be active and safe, suggesting that this chemotherapy-free regimen could become a new first-line treatment approach in patients with DLBCL-RT., Funding: Roche., Competing Interests: Declaration of interests AT reports payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from Janssen, Beigene, and Abbvie; support for attending meetings or travel from Abbvie, Janssen, and Beigene; and participation on a data safety monitoring board or advisory board from Janssen, Abbvie, Beigene, Lilly, and AstraZeneca. AMF reports consulting fees from Janssen; payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from Janssen, Beigene, and AstraZeneca; support for attending meetings or travel from Abbvie, Janssen, Beigene, and AstraZeneca; and participation on a data safety monitoring board or advisory board from Janssen, Beigene, and AstraZeneca. ACo reports grants or contracts from Gilead, BMS, Beigene, Abbvie, Janssen-Cilag, and AstraZeneca; payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from AstraZeneca, Abbvie, and Janssen-Cilag; support for attending meetings or travel from Janssen; and participation on a data safety monitoring board or advisory board from BMS. MC reports grants or contracts from Johnson & Johnson and Abbvie; payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from Abbvie and AstraZeneca; support for attending meetings or travel from Johnson & Johnson, Abbvie, and AstraZeneca; and participation on a data safety monitoring board or advisory board from Johnson & Johnson, Abbvie, AstraZeneca, Beigene, and GSK. RC reports support for the present manuscript from the US National Institutes of Health and National Cancer Institute (R01CA196703–01). PLZ reports consulting fees and payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from Takeda, BMS, MSD, Roche, Gilead, Novartis, Abbvie, Beigene, Kyowa Kirin, and Janssen. MMot reports payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events; support for attending meetings or travel; and participation on a data safety monitoring board or advisory board from Abbvie, Janssen, and AstraZeneca. GG reports grants from Associazione Italiana per la Ricerca sul Cancro and Piano Nazionale di Ripresa e Resilienza; consulting fees from Abbvie, AstraZeneca, Beiene, Hikma, and Johnson & Johnson; and participation on a data safety monitoring board or advisory board from Abbvie, AstraZeneca, Beigene, Hikma, Johnson & Johnson, and Lilly. LS reports consulting from Abbvie, Beigene, Lilly, AstraZeneca, Janssen, and Merck; payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from Abbvie, Beigene, Lilly, Octapharma, AstraZeneca, Janssen, and Merck; support for attending meetings or travel from AstraZeneca, Janssen, and Beigene; and participation on a data safety monitoring board or advisory board from Merck. RCa reports payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from Abbvie, Gilead, Gentilipharma, Pierre Fabre, Celgene, and Menarini stemline; support for attending meetings or travel from Pierre Fabre, Beigene, and Servier; and participation on a data safety monitoring board or advisory board from Celgene, Abbvie, Gentilipharma, and Daichi Samkio. TZ report consulting fees and payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from Roche, Novartis, Gilead, Janssen, AstraZeneca, Lilly, and Abbvie. EZ reports consulting fees from Abbvie, Beigene, BMS, Cures, Elly and Lilly, Incyte, Ipsen, Merck, Miltenyibiomedicine, and Roche; payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from Abbvie, AstraZeneca, Beigene, and Gilead; support for attending meetings or travel from Abbvie; and participation on a data safety monitoring board or advisory board from Merck. DR reports support for the present manuscript from Adaptive; grants or contracts, consulting fees, payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing, or educational events from Abbvie, AstraZeneca, Beigene, BMS, Janssen, and Lilly. All other authors declare no competing interests. No honoraria or payments were made for authorship., (Copyright © Elsevier Ltd. All rights reserved, including those for text and data mining, AI training, and similar technologies.)
- Published
- 2024
- Full Text
- View/download PDF
20. Hairy cell leukemia with an atypical extranodal presentation: A clinicopathological analysis of four cases.
- Author
-
Sangiorgio V, Palasciano A, Tabanelli V, Giné E, Guerra L, Pagni F, Casiraghi A, Casaroli I, Frigola G, Magnano L, Gambacorti-Passerini C, Derenzini E, Vanazzi A, and Campo E
- Subjects
- Humans, Male, Middle Aged, Aged, Female, Biomarkers, Tumor analysis, Adult, Immunohistochemistry, Biopsy, Leukemia, Hairy Cell pathology, Leukemia, Hairy Cell diagnosis
- Abstract
Competing Interests: Declaration of competing interest All authors report no relevant potential conflicts of interest.
- Published
- 2024
- Full Text
- View/download PDF
21. Concomitant cutaneous T-cell lymphoma and biclonal B-cell lymphoproliferative disorder.
- Author
-
Derosa C, Mestice A, Perrone T, Ingravallo G, Troia M, Tabanelli V, Filotico R, and Musto P
- Published
- 2024
- Full Text
- View/download PDF
22. A targeted gene signature stratifying mediastinal gray zone lymphoma into classical HL-like or PMBL-like subtypes.
- Author
-
Gargano G, Vegliante MC, Esposito F, Pappagallo SA, Sabattini E, Agostinelli C, Pileri SA, Tabanelli V, Ponzoni M, Lorenzi L, Facchetti F, Di Napoli A, Lucioni M, Paulli M, Leoncini L, Lazzi S, Ascani S, Opinto G, Zaccaria GM, Volpe G, Mondelli P, Bucci A, Selicato L, Negri A, Loseto G, Clemente F, Scattone A, Zito AF, Nassi L, Del Buono N, Guarini A, and Ciavarella S
- Abstract
Not available.
- Published
- 2024
- Full Text
- View/download PDF
23. Lymphomatoid Papulosis With T-cell Receptor-Gamma Delta Expression: A Clinicopathologic Case-series of 26 Patients of an Underrecognized Immunophenotypic Variant of Lymphomatoid Papulosis.
- Author
-
Mark E, Kempf W, Guitart J, Pulitzer M, Mitteldorf C, Hristov A, Torres-Cabala C, Marchi E, Cropley T, Rodriguez Pinilla SM, Griffin T, Fernandez R, Pileri S, Pileri A, Tabanelli V, Borretta L, Subtil A, Plaza JA, Piris JAMA, Feldman AL, Cerroni L, and Gru AA
- Subjects
- Humans, Receptors, Antigen, T-Cell, Lymphomatoid Papulosis pathology, Skin Neoplasms pathology, Mycosis Fungoides pathology, Lymphoma, T-Cell, Cutaneous
- Abstract
Lymphomatoid papulosis (LyP) has several histopathologic presentations. LyP featuring gamma-delta (γδ) T-cell receptor expression may masquerade as and may be misdiagnosed as aggressive cutaneous T-cell lymphoma, particularly primary cutaneous γδ T-cell lymphoma (PCGDTL) or γδ mycosis fungoides. We performed a clinicopathologic analysis of the largest series of LyP featuring γδ T-cell expression. We identified 26 patients with a diagnosis of LyP with γδ T cells from our institutions, as well as through a comprehensive review of the literature, and characterized these cases. Most cases were treated with topical steroids or not treated at all. The majority of cases showed a CD4 - CD8 + phenotype and featured at least one cytotoxic marker. Histopathologic features included an intraepidermal or dermal infiltrate with large cells and frequent angiotropism. One case was initially misdiagnosed as PCGDTL, requiring further therapy. Our case series, the largest international cohort of γδ T cell predominant LyP cases, confirms marked clinicopathologic heterogeneity that may contribute to misdiagnosis, reasserting the need to identify classic clinical features, CD30 + T-cell components, and markers of cytotoxicity when dealing with this differential diagnosis. A limitation of this study includes somewhat limited follow-up, histologic, and immunophenotypic information for some cases., Competing Interests: Conflicts of Interest and Source of Funding: A.L.F. receives research funding from Seattle Genetics, is an inventor of technology for which Mayo Clinic holds an unlicensed patent, and has intellectual property licensed to Zeno Pharmaceuticals. A.A.G. is an investigator for StemLine Therapeutics, Innate Pharma, and Dren Bio. He is also consultant for Kyowa Kirin and BluePrints Medicine. For the remaining authors none were declared., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
24. A Fondazione Italiana Linfomi cohort study of R-COMP vs R-CHOP in older patients with diffuse large B-cell lymphoma.
- Author
-
Arcari A, Rigacci L, Tucci A, Puccini B, Usai SV, Cavallo F, Fabbri A, Balzarotti M, Pelliccia S, Luminari S, Pennese E, Zilioli VR, Mahmoud AM, Musuraca G, Marino D, Sartori R, Botto B, Gini G, Zanni M, Hohaus S, Tarantini G, Flenghi L, Tani M, Di Rocco A, Merli M, Vallisa D, Pagani C, Nassi L, Dessì D, Ferrero S, Cencini E, Bernuzzi P, Mammi C, Marcheselli L, Tabanelli V, Spina M, and Merli F
- Subjects
- Aged, Humans, Rituximab adverse effects, Vincristine adverse effects, Cohort Studies, Prospective Studies, Prednisone adverse effects, Treatment Outcome, Doxorubicin adverse effects, Cyclophosphamide adverse effects, Antineoplastic Combined Chemotherapy Protocols adverse effects, Lymphoma, Large B-Cell, Diffuse pathology, Heart Diseases etiology
- Abstract
Rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) is the most commonly used regimen for the upfront treatment of diffuse large B-cell lymphoma (DLBCL). However, it is associated with cardiotoxicity, especially in older patients. Substituting doxorubicin with non-PEGylated liposomal doxorubicin (R-COMP) may reduce the risk of cardiac events, but its efficacy has never been demonstrated in prospective trials. We describe the characteristics and outcome of patients with DLBCL aged ≥65 years prospectively enrolled in the Elderly Project by the Fondazione Italiana Linfomi and treated with full doses of R-CHOP or R-COMP per local practice. Starting from 1163 patients, 383 (55%) were treated with R-CHOP and 308 (45%) with R-COMP. Patients treated with R-COMP were older (median age, 76 vs 71 years), less frequently fit at simplified geriatric assessment (61% vs 88%; P < .001), and had a more frequent baseline cardiac disorders (grade >1, 32% vs 8%; P < .001). Three-year progression-free survival (PFS) was similar between R-CHOP and R-COMP (70% and 64%); 3-year overall survival was 77%, and 71% respectively. R-CHOP was associated with better PFS vs R-COMP only in the Elderly Prognostic Index (EPI) low-risk group. The two groups had similar rates of treatment interruptions due to toxicities or of cardiac events (P = 1.00). We suggest R-COMP is a potentially curative treatment for older patients with intermediate- or high-risk EPI, even in the presence of a baseline cardiopathy. R-CHOP is confirmed as the standard therapy for low risk patients., (© 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
25. Biological features and outcome of diffuse large B-cell lymphoma associated with hepatitis C virus in elderly patients: Results of the prospective 'Elderly Project' by the Fondazione Italiana Linfomi.
- Author
-
Arcari A, Tabanelli V, Merli F, Marcheselli L, Merli M, Balzarotti M, Zilioli VR, Fabbri A, Cavallo F, Casaluci GM, Tucci A, Puccini B, Pennese E, Di Rocco A, Zanni M, Flenghi L, Gini G, Sartori R, Chiappella A, Usai SV, Tani M, Marino D, Arcaini L, Vallisa D, and Spina M
- Subjects
- Aged, Humans, Hepacivirus genetics, Antiviral Agents therapeutic use, Prospective Studies, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Neoplasm Recurrence, Local drug therapy, Rituximab therapeutic use, Doxorubicin therapeutic use, Vincristine therapeutic use, Cyclophosphamide therapeutic use, Prednisone therapeutic use, Hepatitis C, Chronic drug therapy, Hepatitis C drug therapy, Hepatitis C epidemiology, Lymphoma, Large B-Cell, Diffuse
- Abstract
Up to 10%-15% of diffuse large B-cell lymphoma (DLBCL) are related to hepatitis C virus (HCV) infection, in particular in elderly patients. The Fondazione Italiana Linfomi has recently published a multicentre prospective observational study, the 'Elderly Project', on the outcome of DLBCL in patients aged ≥65 years, evaluated using a simplified comprehensive geriatric assessment. The aim of this study was to compare biological and clinical features of HCV positive (HCV+) with HCV negative (HCV-) cases. A total of 89 HCV+ patients were identified out of 1095 evaluated for HCV serology (8.1%). The HCV+ patients were older, less fit, and had frequent extranodal involvement. The cell-of-origin determination by Nanostring showed that HCV+ cases less frequently had an activated B-cell profile compared to HCV- patients (18% vs. 43%). In all, 86% of HCV+ patients received rituximab-cyclophosphamide, doxorubicin, vincristine (Oncovin) and prednisone (R-CHOP)-like immunochemotherapy. Grade 3-4 liver toxicity occurred in 3% of cases. Among centrally reviewed cases confirmed as DLBCL, the 3-year overall survival of HCV+ patients was very similar to HCV- (63% vs. 61%, p = 0.926). In all, 20 HCV+ patients were treated with direct-acting antiviral agents (DAAs), with good tolerance and sustained virological response in all cases. The 3-year progression-free survival for this subgroup was excellent (77%), suggesting DAAs' possible role in reducing the risk of relapse by eliminating the viral trigger., (© 2023 British Society for Haematology and John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
26. Gene expression profiling and FDG-PET radiomics uncover radiometabolic signatures associated with outcome in DLBCL.
- Author
-
Mazzara S, Travaini L, Botta F, Granata C, Motta G, Melle F, Fiori S, Tabanelli V, Vanazzi A, Ramadan S, Radice T, Raimondi S, Lo Presti G, Ferrari ME, Jereczek-Fossa BA, Tarella C, Ceci F, Pileri S, and Derenzini E
- Subjects
- Humans, Retrospective Studies, Positron-Emission Tomography methods, Gene Expression Profiling, Fluorodeoxyglucose F18, Lymphoma, Large B-Cell, Diffuse pathology
- Abstract
Emerging evidence indicates that chemoresistance is closely related to altered metabolism in cancer. Here, we hypothesized that distinct metabolic gene expression profiling (GEP) signatures might be correlated with outcome and with specific fluorodeoxyglucose positron emission tomography (FDG-PET) radiomic profiles in diffuse large B-cell lymphoma (DLBCL). We retrospectively analyzed a discovery cohort of 48 consecutive patients with DLBCL treated at our center with standard first-line chemoimmunotherapy by performing targeted GEP (T-GEP)- and FDG-PET radiomic analyses on the same target lesions at baseline. T-GEP-based metabolic profiling identified a 6-gene signature independently associated with outcomes in univariate and multivariate analyses. This signature included genes regulating mitochondrial oxidative metabolism (SCL25A1, PDK4, PDPR) that were upregulated and was inversely associated with genes involved in hypoxia and glycolysis (MAP2K1, HIF1A, GBE1) that were downregulated. These data were validated in 2 large publicly available cohorts. By integrating FDG-PET radiomics and T-GEP, we identified a radiometabolic signature (RadSig) including 4 radiomic features (histo kurtosis, histo energy, shape sphericity, and neighboring gray level dependence matrix contrast), significantly associated with the metabolic GEP-based signature (r = 0.43, P = .0027) and with progression-free survival (P = .028). These results were confirmed using different target lesions, an alternative segmentation method, and were validated in an independent cohort of 64 patients. RadSig retained independent prognostic value in relation to the International Prognostic Index score and metabolic tumor volume (MTV). Integration of RadSig and MTV further refined prognostic stratification. This study provides the proof of principle for the use of FDG-PET radiomics as a tool for noninvasive assessment of cancer metabolism and prognostic stratification in DLBCL., (© 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
27. Gene Expression Signatures for the Accurate Diagnosis of Peripheral T-Cell Lymphoma Entities in the Routine Clinical Practice.
- Author
-
Amador C, Bouska A, Wright G, Weisenburger DD, Feldman AL, Greiner TC, Lone W, Heavican T, Smith L, Pileri S, Tabanelli V, Ott G, Rosenwald A, Savage KJ, Slack G, Kim WS, Hyeh Y, Li Y, Dong G, Song J, Ondrejka S, Cook JR, Barrionuevo C, Lim ST, Ong CK, Chapman J, Inghirami G, Raess PW, Bhagavathi S, Gould C, Blombery P, Jaffe E, Morris SW, Rimsza LM, Vose JM, Staudt L, Chan WC, and Iqbal J
- Subjects
- Humans, Transcriptome, Reproducibility of Results, Gene Expression Profiling, Prognosis, Lymphoma, T-Cell, Peripheral diagnosis, Lymphoma, T-Cell, Peripheral genetics
- Abstract
Purpose: Peripheral T-cell lymphoma (PTCL) includes heterogeneous clinicopathologic entities with numerous diagnostic and treatment challenges. We previously defined robust transcriptomic signatures that distinguish common PTCL entities and identified two novel biologic and prognostic PTCL-not otherwise specified subtypes (PTCL-TBX21 and PTCL-GATA3). We aimed to consolidate a gene expression-based subclassification using formalin-fixed, paraffin-embedded (FFPE) tissues to improve the accuracy and precision in PTCL diagnosis., Materials and Methods: We assembled a well-characterized PTCL training cohort (n = 105) with gene expression profiling data to derive a diagnostic signature using fresh-frozen tissue on the HG-U133plus2.0 platform (Affymetrix, Inc, Santa Clara, CA) subsequently validated using matched FFPE tissues in a digital gene expression profiling platform (nCounter, NanoString Technologies, Inc, Seattle, WA). Statistical filtering approaches were applied to refine the transcriptomic signatures and then validated in another PTCL cohort (n = 140) with rigorous pathology review and ancillary assays., Results: In the training cohort, the refined transcriptomic classifier in FFPE tissues showed high sensitivity (> 80%), specificity (> 95%), and accuracy (> 94%) for PTCL subclassification compared with the fresh-frozen-derived diagnostic model and showed high reproducibility between three independent laboratories. In the validation cohort, the transcriptional classifier matched the pathology diagnosis rendered by three expert hematopathologists in 85% (n = 119) of the cases, showed borderline association with the molecular signatures in 6% (n = 8), and disagreed in 8% (n = 11). The classifier improved the pathology diagnosis in two cases, validated by clinical findings. Of the 11 cases with disagreements, four had a molecular classification that may provide an improvement over pathology diagnosis on the basis of overall transcriptomic and morphological features. The molecular subclassification provided a comprehensive molecular characterization of PTCL subtypes, including viral etiologic factors and translocation partners., Conclusion: We developed a novel transcriptomic approach for PTCL subclassification that facilitates translation into clinical practice with higher precision and uniformity than conventional pathology diagnosis.
- Published
- 2022
- Full Text
- View/download PDF
28. Dampening of cytotoxic innate lymphoid cells: A new tumour immune escape mechanism in B cell non-Hodgkin's lymphoma.
- Author
-
Roma S, Camisaschi C, Mancuso P, Trabanelli S, Vanazzi A, Villa S, Prati D, Fiori S, Lorenzini D, Tabanelli V, Pileri S, Tarella C, Jandus C, and Bertolini F
- Subjects
- Humans, Tumor Escape, Immunity, Innate, Lymphocytes, Killer Cells, Natural, Antineoplastic Agents, Neoplasms pathology, Lymphoma, Non-Hodgkin pathology
- Abstract
The role and regulation of innate immune cells is poorly understood in B-cell non-Hodgkin lymphoma (NHL). As natural killer (NK) cells, helper innate lymphoid cells (ILCs) are lymphocytes endowed with either anti- or pro-tumour activity and involved in inflammatory processes. In our ex vivo analysis of NK cells and ILCs from NHL patients, we observed that, in comparison to healthy donors (HD), the frequency of the cytotoxic subset of NK cells, the CD16
+ NK, decreased in patients' peripheral blood. In general, circulating NK cells showed a pro-tumorigenic phenotype, while ILCs displayed a more activated/cytotoxic phenotype. Conversely, at the tumour site, in patients' lymph nodes, ILCs showed a low expression of granzyme.In vitromixed lymphocyte-tumour cell cultures with HD PBMCs and NHL cell lines demonstrated that ILC cytotoxic potential was lowered by the presence of tumour cells but, in the absence of T regulatory cells (Tregs), their cytolytic potential was recovered. Our data shed novel light on dysfunctional innate immunity in NHL. We suggest a new mechanism of tumour immuno-escape based on the reduction of cell cytotoxicity involving ILCs and likely controlled by Tregs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)- Published
- 2022
- Full Text
- View/download PDF
29. NR1H3 (LXRα) is associated with pro-inflammatory macrophages, predicts survival and suggests potential therapeutic rationales in diffuse large b-cell lymphoma.
- Author
-
Vegliante MC, Mazzara S, Zaccaria GM, De Summa S, Esposito F, Melle F, Motta G, Sapienza MR, Opinto G, Volpe G, Bucci A, Gargano G, Enjuanes A, Tabanelli V, Fiori S, Minoia C, Clemente F, Negri A, Gulino A, Morello G, Scattone A, Zito AF, Tommasi S, Agostinelli C, Vitolo U, Chiappella A, Barbui AM, Derenzini E, Zinzani PL, Casadei B, Rivas-Delgado A, López-Guillermo A, Campo E, Moschetta A, Guarini A, Pileri SA, and Ciavarella S
- Subjects
- Humans, Tumor Microenvironment, Liver X Receptors genetics, Lymphoma, Large B-Cell, Diffuse drug therapy, Lymphoma, Large B-Cell, Diffuse genetics
- Abstract
The role of macrophages (Mo) and their prognostic impact in diffuse large B-cell lymphomas (DLBCL) remain controversial. By regulating the lipid metabolism, Liver-X-Receptors (LXRs) control Mo polarization/inflammatory response, and their pharmacological modulation is under clinical investigation to treat human cancers, including lymphomas. Herein, we surveyed the role of LXRs in DLBCL for prognostic purposes. Comparing bulk tumors with purified malignant and normal B-cells, we found an intriguing association of NR1H3, encoding for the LXR-α isoform, with the tumor microenvironment (TME). CIBERSORTx-based purification on large DLBCL datasets revealed a high expression of the receptor transcript in M1-like pro-inflammatory Mo. By determining an expression cut-off of NR1H3, we used digital measurement to validate its prognostic capacity on two large independent on-trial and real-world cohorts. Independently of classical prognosticators, NR1H3
high patients displayed longer survival compared with NR1H3low cases and a high-resolution Mo GEP dissection suggested a remarkable transcriptional divergence between subgroups. Overall, our findings indicate NR1H3 as a Mo-related biomarker identifying patients at higher risk and prompt future preclinical studies investigating its mouldability for therapeutic purposes., (© 2022 The Authors. Hematological Oncology published by John Wiley & Sons Ltd.)- Published
- 2022
- Full Text
- View/download PDF
30. The identification of TCF1+ progenitor exhausted T cells in THRLBCL may predict a better response to PD-1/PD-L1 blockade.
- Author
-
Tabanelli V, Melle F, Motta G, Mazzara S, Fabbri M, Agostinelli C, Calleri A, Del Corvo M, Fiori S, Lorenzini D, Cesano A, Chiappella A, Vitolo U, Derenzini E, Griffin GK, Rodig SJ, Vanazzi A, Sabattini E, Tarella C, Sapienza MR, and Pileri SA
- Subjects
- Apoptosis, Hepatocyte Nuclear Factor 1-alpha, Histiocytes metabolism, Histiocytes pathology, Humans, Ligands, Programmed Cell Death 1 Receptor, Tumor Microenvironment, B7-H1 Antigen genetics, Lymphoma, Large B-Cell, Diffuse drug therapy, Lymphoma, Large B-Cell, Diffuse genetics
- Abstract
T-cell/histiocyte-rich large B-cell lymphoma (THRLBCL) is a rare and aggressive variant of diffuse large B-cell lymphoma (DLBCL) that usually affects young to middle-aged patients, with disseminated disease at presentation. The tumor microenvironment (TME) plays a key role in THRLBCL due to its peculiar cellular composition (<10% neoplastic B cells interspersed in a cytotoxic T-cell/histiocyte-rich background). A significant percentage of THRLBCL is refractory to rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone (RCHOP)-based regimens and to chimeric antigen receptor T-cell therapy; thus, the development of a specific therapeutic approach for these patients represents an unmet clinical need. To better understand the interaction of immune cells in THRLBCL TME and identify more promising therapeutic strategies, we compared the immune gene expression profiles of 12 THRLBCL and 10 DLBCL samples, and further corroborated our findings in an extended in silico set. Gene coexpression network analysis identified the predominant role of the programmed cell death protein 1 (PD-1)/programmed cell death ligand 1 (PD-L1) axis in the modulation of the immune response. Furthermore, the PD-1/PD-L1 activation was flanked by the overexpression of 48 genes related to the functional exhaustion of T cells. Globally, THRLBCL TME was highly interferon-inflamed and severely exhausted. The immune gene profiling findings strongly suggest that THRLBCL may be responsive to anti-PD-1 therapy but also allowed us to take a step forward in understanding THRLBCL TME. Of therapeutic relevance, we validated our results by immunohistochemistry, identifying a subset of TCF1+ (T cell-specific transcription factor 1, encoded by the TCF7 gene) progenitor exhausted T cells enriched in patients with THRLBCL. This subset of TCF1+ exhausted T cells correlates with good clinical response to immune checkpoint therapy and may improve prediction of anti-PD-1 response in patients with THRLBCL., (© 2022 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
31. PD-1 and PD-L1 expression in mycosis fungoides and Sézary Syndrome.
- Author
-
Pileri A, Tabanelli V, Fuligni F, Agostinelli C, Guglielmo A, Sabattini E, Grandi V, Pileri SA, and Pimpinelli N
- Subjects
- Humans, Lymphocytes, Tumor-Infiltrating, B7-H1 Antigen genetics, Mycosis Fungoides metabolism, Programmed Cell Death 1 Receptor genetics, Sezary Syndrome metabolism, Skin Neoplasms metabolism
- Abstract
Background: The mechanisms involved in mycosis fungoides, and Sezary Syndrome progression are largely unknown. Over the last decade the interest in immune system contrast of neoplasm has grown owing to the introduction of immunotherapy. PD-1 and its ligand (PD-L1) are the target of several immunotherapy treatment. In the literature reports on the expression of PD-1 and PD-L1 have provided contrasting results., Methods: In our analysis we investigated PD-1 expression in neoplastic cells and in tumor infiltrating lymphocytes (TILs) as well as PD-L1 expression in tumor cells and in tumor associated macrophages (TAMs). PD-L1 and PD-1 positive cells were counted in 5 high-power fields (HPF) and scored as the average number of positive neoplastic cells/TILs/TAMs per HPF., Results: From databases of two institutions (Bologna and Florence) thirty-five patients corresponding to 43 biopsies were retrieved. In seven instances sequential biopsies were present. No statistically significant expression was observed comparing early to advanced stages by analysing PD-1 by tumor cells and TILs and of PD-L1 by tumor cells and TAMs., Conclusions: Our results corroborate that PD-1 and PD-L1 expression is not stage-dependent in mycosis fungoides and Sezary syndrome. However, PD-1 and PD-L1 expression in affected patients provides a rationale to schedule anti PD-1/PD-L1 drugs.
- Published
- 2022
- Full Text
- View/download PDF
32. Histiocytic and dendritic cell neoplasms.
- Author
-
Pileri SA, Melle F, Motta G, and Tabanelli V
- Subjects
- Histiocytes, Dendritic Cells pathology, Neoplasms pathology
- Abstract
Background: Histiocytic and dendritic cell neoplasms (HDCNs) represent very rare tumors, which have been the subject of debate during the last few years., Objectives: We aimed to provide a comprehensive review of the subject., Materials and Methods: The experience gained by the authors in large international studies and as a national reference center has been summarized to highlight the characteristics of each entity., Results: The clinical, morphologic, phenotypic, and molecular data of the different entities included under the heading of HDCNs are extensively discussed., Conclusion: Currently, HDCNs are classified in the group of orphan diseases for which a standardized therapy is lacking. An international registry would facilitate expansion and dissemination of knowledge of these diseases and improve their treatment., (© 2022. The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature.)
- Published
- 2022
- Full Text
- View/download PDF
33. Dual targeting of the DNA damage response pathway and BCL-2 in diffuse large B-cell lymphoma.
- Author
-
Rossi A, Orecchioni S, Falvo P, Tabanelli V, Baiardi E, Agostinelli C, Melle F, Motta G, Calleri A, Fiori S, Corsini C, Casadei B, Mazzara S, Vitolo U, Bertolini F, Zinzani PL, Alcalay M, Pelicci PG, Pileri S, Tarella C, and Derenzini E
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Antineoplastic Agents pharmacology, Biomarkers, Tumor genetics, Biomarkers, Tumor metabolism, Drug Therapy, Combination, Female, Follow-Up Studies, Humans, Lymphoma, Large B-Cell, Diffuse metabolism, Lymphoma, Large B-Cell, Diffuse pathology, Male, Middle Aged, Prognosis, Prospective Studies, Retrospective Studies, Survival Rate, Urea pharmacology, Young Adult, Bridged Bicyclo Compounds, Heterocyclic pharmacology, DNA Repair Enzymes antagonists & inhibitors, Gene Expression Regulation, Leukemic drug effects, Lymphoma, Large B-Cell, Diffuse drug therapy, Proto-Oncogene Proteins c-bcl-2 antagonists & inhibitors, Sulfonamides pharmacology, Thiophenes pharmacology, Urea analogs & derivatives
- Abstract
Standard chemotherapies for diffuse large B-cell lymphoma (DLBCL), based on the induction of exogenous DNA damage and oxidative stress, are often less effective in the presence of increased MYC and BCL-2 levels, especially in the case of double hit (DH) lymphomas harboring rearrangements of the MYC and BCL-2 oncogenes, which enrich for a patient's population characterized by refractoriness to anthracycline-based chemotherapy. Here we hypothesized that adaptive mechanisms to MYC-induced replicative and oxidative stress, consisting in DNA damage response (DDR) activation and BCL-2 overexpression, could represent the biologic basis of the poor prognosis and chemoresistance observed in MYC/BCL-2-positive lymphoma. We first integrated targeted gene expression profiling (T-GEP), fluorescence in situ hybridization (FISH) analysis, and characterization of replicative and oxidative stress biomarkers in two independent DLBCL cohorts. The presence of oxidative DNA damage biomarkers identified a poor prognosis double expresser (DE)-DLBCL subset, characterized by relatively higher BCL-2 gene expression levels and enrichment for DH lymphomas. Based on these findings, we tested therapeutic strategies based on combined DDR and BCL-2 inhibition, confirming efficacy and synergistic interactions in in vitro and in vivo DH-DLBCL models. These data provide the rationale for precision-therapy strategies based on combined DDR and BCL-2 inhibition in DH or DE-DLBCL., (© 2021. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
34. Electronic case report forms generation from pathology reports by ARGO, automatic record generator for onco-hematology.
- Author
-
Zaccaria GM, Colella V, Colucci S, Clemente F, Pavone F, Vegliante MC, Esposito F, Opinto G, Scattone A, Loseto G, Minoia C, Rossini B, Quinto AM, Angiulli V, Grieco LA, Fama A, Ferrero S, Moia R, Di Rocco A, Quaglia FM, Tabanelli V, Guarini A, and Ciavarella S
- Subjects
- Disease Management, Humans, Natural Language Processing, Workflow, Electronic Health Records, Hematology methods, Hematology standards, Medical Oncology methods, Medical Oncology standards, Research Report
- Abstract
The unstructured nature of Real-World (RW) data from onco-hematological patients and the scarce accessibility to integrated systems restrain the use of RW information for research purposes. Natural Language Processing (NLP) might help in transposing unstructured reports into standardized electronic health records. We exploited NLP to develop an automated tool, named ARGO (Automatic Record Generator for Onco-hematology) to recognize information from pathology reports and populate electronic case report forms (eCRFs) pre-implemented by REDCap. ARGO was applied to hemo-lymphopathology reports of diffuse large B-cell, follicular, and mantle cell lymphomas, and assessed for accuracy (A), precision (P), recall (R) and F1-score (F) on internal (n = 239) and external (n = 93) report series. 326 (98.2%) reports were converted into corresponding eCRFs. Overall, ARGO showed high performance in capturing (1) identification report number (all metrics > 90%), (2) biopsy date (all metrics > 90% in both series), (3) specimen type (86.6% and 91.4% of A, 98.5% and 100.0% of P, 92.5% and 95.5% of F, and 87.2% and 91.4% of R for internal and external series, respectively), (4) diagnosis (100% of P with A, R and F of 90% in both series). We developed and validated a generalizable tool that generates structured eCRFs from real-life pathology reports., (© 2021. The Author(s).)
- Published
- 2021
- Full Text
- View/download PDF
35. Venetoclax Shows Low Therapeutic Activity in BCL2-Positive Relapsed/Refractory Peripheral T-Cell Lymphoma: A Phase 2 Study of the Fondazione Italiana Linfomi.
- Author
-
Ballotta L, Zinzani PL, Pileri S, Bruna R, Tani M, Casadei B, Tabanelli V, Volpetti S, Luminari S, Corradini P, Lucchini E, Tisi MC, Merli M, Re A, Varettoni M, Pesce EA, and Zaja F
- Abstract
Patients with relapsed/refractory (R/R) peripheral T-cell lymphoma (PTCL) have a poor prognosis, with an expected survival of less than 1 year using standard salvage therapies. Recent advances in our understanding of the biology of PTCL have led to identifying B-Cell Lymphoma 2 (BCL2) protein as a potential therapeutic target. BLC2 inhibitor venetoclax was investigated in a prospective phase II trial in patients with BCL2-positive R/R PTCL after at least one previous standard line of treatment (NCT03552692). Venetoclax given alone at a dosage of 800 mg/day resulted in one complete response (CR) and two stable diseases (SDs) among 17 enrolled patients. The majority of patients (88.2%) interrupted the treatment due to disease progression. No relationship with BCL2 expression was documented. At a median follow-up of 8 months, two patients are currently still on treatment (one CR and one SD). No case of tumor lysis syndrome was registered. Therefore, venetoclax monotherapy shows activity in a minority of patients whose biological characteristics have not yet been identified., Clinical Trial Registration: www.clinicaltrials.gov (NCT03552692, EudraCT number 2017-004630-29)., Competing Interests: PZ has received advisory board fees from Secura Bio, Celltrion, Gilead, Janssen-Cilag, BMS, Servier, Sandoz, MSD, TG Therapeutics, Takeda, Roche, Eusapharma, Kyowa Kirin, Novartis, ADC Therapeutics, Incyte, and Beigene; received consultant fees from MSD, Eusapharma, and Novartis; and received speaker’s bureau fees from Celltrion, Gilead, Janssen-Cilag, BMS, Servier, MSD, TG Therapeutics, Takeda, Roche, Eusapharma, Kyowa Kirin, Novartis, Incyte, and Beigene. SL has received advisory board fees from Roche, BMS/Celgene, Gilead/kite, Jannsen, Genmab, and Regeneron. MV has received advisory board fees from Janssen, Beigene, and Roche and travel expenses from Abbvie. FZ has received advisory board fees from Roche, Celgene, Janssen, Sandoz, Gilead, Novartis, Abbvie, Amgen, Sobi, Argenx, and Grifols and received honoraria for giving lectures to medical meetings from Celgene, Janssen, Gilead, Novartis, Roche, Amgen, Abbvie, and Grifols. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Ballotta, Zinzani, Pileri, Bruna, Tani, Casadei, Tabanelli, Volpetti, Luminari, Corradini, Lucchini, Tisi, Merli, Re, Varettoni, Pesce and Zaja.)
- Published
- 2021
- Full Text
- View/download PDF
36. Newly-Discovered Neural Features Expand the Pathobiological Knowledge of Blastic Plasmacytoid Dendritic Cell Neoplasm.
- Author
-
Sapienza MR, Benvenuto G, Ferracin M, Mazzara S, Fuligni F, Tripodo C, Belmonte B, Fanoni D, Melle F, Motta G, Tabanelli V, Consiglio J, Mazzara V, Del Corvo M, Fiori S, Pileri A, Dellino GI, Cerroni L, Facchetti F, Berti E, Sabattini E, Paulli M, Croce CM, and Pileri SA
- Abstract
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and highly aggressive hematologic malignancy originating from plasmacytoid dendritic cells (pDCs). The microRNA expression profile of BPDCN was compared to that of normal pDCs and the impact of miRNA dysregulation on the BPDCN transcriptional program was assessed. MiRNA and gene expression profiling data were integrated to obtain the BPDCN miRNA-regulatory network. The biological process mainly dysregulated by this network was predicted to be neurogenesis, a phenomenon raising growing interest in solid tumors. Neurogenesis was explored in BPDCN by querying different molecular sources (RNA sequencing, Chromatin immunoprecipitation-sequencing, and immunohistochemistry). It was shown that BPDCN cells upregulated neural mitogen genes possibly critical for tumor dissemination, expressed neuronal progenitor markers involved in cell migration, exchanged acetylcholine neurotransmitter, and overexpressed multiple neural receptors that may stimulate tumor proliferation, migration and cross-talk with the nervous system. Most neural genes upregulated in BPDCN are currently investigated as therapeutic targets.
- Published
- 2021
- Full Text
- View/download PDF
37. Peripheral T-Cell Lymphoma, Not Otherwise Specified: Clinical Manifestations, Diagnosis, and Future Treatment.
- Author
-
Pileri SA, Tabanelli V, Fiori S, Calleri A, Melle F, Motta G, Lorenzini D, Tarella C, and Derenzini E
- Abstract
Peripheral T-cell lymphoma, not otherwise specified (PTCL_NOS) corresponds to about one fourth of mature T-cell tumors, which overall represent 10-12% of all lymphoid malignancies. This category comprises all T-cell neoplasms, which do not correspond to any of the distinct entities listed in the WHO (World Health Organization) Classification of Tumours of Haematopoietic and Lymphoid Tissues. In spite of the extreme variability of morphologic features and phenotypic profiles, gene expression profiling (GEP) studies have shown a signature that is distinct from that of all remaining PTCLs. GEP has also allowed the identification of subtypes provided with prognostic relevance. Conversely to GEP, next-generation sequencing (NGS) has so far been applied to a limited number of cases, providing some hints to better understand the pathobiology of PTCL_NOS. Although several pieces of information have emerged from pathological studies, PTCL_NOS still remains a tumor with a dismal prognosis. The usage of CHOEP (cyclophosphamide, doxorubicin, vincristine, prednisone, etoposide) followed by autologous stem cell transplantation may represent the best option, by curing about 50% of the patients whom such an approach can be applied to. Many new drugs have been proposed without achieving the expected results. Thus, the optimal treatment of PTCL_NOS remains unidentified.
- Published
- 2021
- Full Text
- View/download PDF
38. A three-gene signature based on MYC , BCL-2 and NFKBIA improves risk stratification in diffuse large B-cell lymphoma.
- Author
-
Derenzini E, Mazzara S, Melle F, Motta G, Fabbri M, Bruna R, Agostinelli C, Cesano A, Corsini CA, Chen N, Righi S, Sabattini E, Chiappella A, Calleri A, Fiori S, Tabanelli V, Cabras A, Pruneri G, Vitolo U, Gianni AM, Rambaldi A, Corradini P, Zinzani PL, Tarella C, and Pileri S
- Subjects
- Gene Expression Profiling, Humans, NF-KappaB Inhibitor alpha, Prognosis, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-6 genetics, Proto-Oncogene Proteins c-myc genetics, Risk Assessment, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Lymphoma, Large B-Cell, Diffuse diagnosis, Lymphoma, Large B-Cell, Diffuse drug therapy, Lymphoma, Large B-Cell, Diffuse genetics
- Abstract
Recent randomized trials focused on gene expression-based determination of the cell of origin in diffuse large B-cell lymphoma could not show significant improvements by adding novel agents to standard chemoimmunotherapy. The aim of this study was the identification of a gene signature able to refine current prognostication algorithms and applicable to clinical practice. Here we used a targeted gene expression profiling panel combining the Lymph2Cx signature for cell of origin classification with additional targets including MYC, BCL-2 and NFKBIA, in 186 patients from 2 randomized trials (discovery cohort) (NCT00355199 and NCT00499018). Data were validated in 3 independent series (2 large public datasets and a real-life cohort). By integrating the cell of origin, MYC/BCL-2 double expressor status and NFKBIA expression, we defined a 3-gene signature combining MYC, BCL-2 and NFKBIA (MBN-signature), which outperformed the MYC/BCL-2 double expressor status in multivariate analysis, and allowed further risk stratification within the germinal center B-cell/unclassified subset. The high-risk (MBN Sig-high) subgroup identified the vast majority of double hit cases and a significant fraction of Activated B-Cell-derived diffuse large B-cell lymphomas. These results were validated in 3 independent series including a cohort from the REMoDL-B trial, where, in an exploratory ad hoc analysis, the addition of bortezomib in the MBN Sig-high subgroup provided a progression free survival advantage compared with standard chemoimmunotherapy. These data indicate that a simple 3-gene signature based on MYC, BCL-2 and NFKBIA could refine the prognostic stratification in diffuse large B-cell lymphoma, and might be the basis for future precision-therapy approaches.
- Published
- 2021
- Full Text
- View/download PDF
39. Pre-existing immune checkpoints activation predicts relapse after allogeneic stem cell transplantation in lymphoma.
- Author
-
Derenzini E, Tabanelli V, Sammassimo S, Mazzara S, Motta G, Melle F, Vanazzi A, Calleri A, Fiori S, Finazzi MC, Barbanti MC, Ramadan S, Gandini S, Pastano R, Rambaldi A, Pileri S, and Tarella C
- Subjects
- Humans, Neoplasm Recurrence, Local, Stem Cell Transplantation, Hematopoietic Stem Cell Transplantation, Lymphoma therapy
- Published
- 2021
- Full Text
- View/download PDF
40. MRI features of breast implant-associated anaplastic large cell lymphoma.
- Author
-
Rotili A, Ferrari F, Nicosia L, Pesapane F, Tabanelli V, Fiori S, Vanazzi A, Meneghetti L, Abbate F, Latronico A, and Cassano E
- Subjects
- Breast diagnostic imaging, Female, Humans, Breast Implants adverse effects, Breast Neoplasms diagnostic imaging, Breast Neoplasms etiology, Lymphoma, Large-Cell, Anaplastic diagnostic imaging, Lymphoma, Large-Cell, Anaplastic etiology, Magnetic Resonance Imaging methods
- Abstract
Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is a rare and newly recognized subtype of T cell Non-Hodgkin Lymphoma (NHLs) associated with breast implants.The mechanism involved in the development of this kind of lymphoma is still uncertain.BIA-ALCL is generally an indolent disease localized to the breast implant and its capsule and effectively treated with capsulectomy alone without chemotherapy.Clinically, BIA-ALCL may typically present a sudden-onset breast-swelling secondary to periimplant effusion. The minority of BIA-ALCL patients present a more aggressive mass-forming subtype, for which systemic therapy is mandatory.Despite the number of cases has recently increased, BIA-ALCL remains a rare disease described mainly in several case reports and small case series.Breast imaging, including mammography, ultrasound and breast MRI are routinely used in the screening of breast cancer; however, guidelines for the imaging and pathological diagnosis of this disease have only recently been proposed and included in the 2019 National Comprehensive Cancer Network (NCCN) consensus guidelines for BIA-ALCL.The main purpose of this pictorial is to illustrate the MRI signs of BIA-ALCL and correlate them with the corresponding pathology features in order to improve the knowledge of the principals MRI features of this type of lymphoma.
- Published
- 2021
- Full Text
- View/download PDF
41. Predictive and Prognostic Molecular Factors in Diffuse Large B-Cell Lymphomas.
- Author
-
Pileri SA, Tripodo C, Melle F, Motta G, Tabanelli V, Fiori S, Vegliante MC, Mazzara S, Ciavarella S, and Derenzini E
- Subjects
- Humans, Lymphoma, Large B-Cell, Diffuse therapy, Microarray Analysis methods, Prognosis, Gene Expression Profiling methods, High-Throughput Nucleotide Sequencing methods, Lymphoma, Large B-Cell, Diffuse diagnosis, Lymphoma, Large B-Cell, Diffuse genetics, Tumor Microenvironment genetics
- Abstract
Diffuse large B-cell lymphoma (DLBCL) is the commonest form of lymphoid malignancy, with a prevalence of about 40% worldwide. Its classification encompasses a common form, also termed as "not otherwise specified" (NOS), and a series of variants, which are rare and at least in part related to viral agents. Over the last two decades, DLBCL-NOS, which accounts for more than 80% of the neoplasms included in the DLBCL chapter, has been the object of an increasing number of molecular studies which have led to the identification of prognostic/predictive factors that are increasingly entering daily practice. In this review, the main achievements obtained by gene expression profiling (with respect to both neoplastic cells and the microenvironment) and next-generation sequencing will be discussed and compared. Only the amalgamation of molecular attributes will lead to the achievement of the long-term goal of using tailored therapies and possibly chemotherapy-free protocols capable of curing most (if not all) patients with minimal or no toxic effects.
- Published
- 2021
- Full Text
- View/download PDF
42. MicroRNA profiling of blastic plasmacytoid dendritic cell neoplasm and myeloid sarcoma.
- Author
-
Sapienza MR, Fuligni F, Melle F, Tabanelli V, Indio V, Laginestra MA, Motta G, Mazzara S, Cerroni L, Pileri A, Facchetti F, Paulli M, Cascione L, Laganà A, Berti E, Ferracin M, Agostinelli C, Sabattini E, Croce CM, and Pileri SA
- Subjects
- Blast Crisis metabolism, Hematologic Neoplasms pathology, Humans, Plasmacytoma metabolism, Sarcoma, Myeloid mortality, Survival Analysis, Blast Crisis genetics, Dendritic Cells metabolism, Hematologic Neoplasms genetics, MicroRNAs metabolism, Plasmacytoma genetics, Sarcoma, Myeloid genetics
- Published
- 2020
- Full Text
- View/download PDF
43. Evolutionary crossroads: morphological heterogeneity reflects divergent intra-clonal evolution in a case of high-grade B-cell lymphoma.
- Author
-
Tabanelli V, Melle F, Motta G, Mazzara S, Fabbri M, Corsini C, Gerbino E, Calleri A, Sapienza MR, Abbene I, Stufano V, Barberis M, and Pileri SA
- Subjects
- Clonal Evolution genetics, Humans, Lymphoma, B-Cell diagnosis, Lymphoma, B-Cell genetics
- Published
- 2020
- Full Text
- View/download PDF
44. Correction: Whole exome sequencing reveals mutations in FAT1 tumor suppressor gene clinically impacting on peripheral T-cell lymphoma not otherwise specified.
- Author
-
Laginestra MA, Cascione L, Motta G, Fuligni F, Agostinelli C, Rossi M, Sapienza MR, Righi S, Broccoli A, Indio V, Melle F, Tabanelli V, Calleri A, Novero D, Facchetti F, Inghirami G, Sabattini E, Bertoni F, and Pileri SA
- Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
- Published
- 2020
- Full Text
- View/download PDF
45. Whole exome sequencing reveals mutations in FAT1 tumor suppressor gene clinically impacting on peripheral T-cell lymphoma not otherwise specified.
- Author
-
Laginestra MA, Cascione L, Motta G, Fuligni F, Agostinelli C, Rossi M, Sapienza MR, Righi S, Broccoli A, Indio V, Melle F, Tabanelli V, Calleri A, Novero D, Facchetti F, Inghirami G, Sabattini E, Bertoni F, and Pileri SA
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Female, Genetic Predisposition to Disease, Humans, Lymphoma, T-Cell, Peripheral mortality, Lymphoma, T-Cell, Peripheral therapy, Male, Middle Aged, Phenotype, Prognosis, Young Adult, Biomarkers, Tumor genetics, Cadherins genetics, Genes, Tumor Suppressor, High-Throughput Nucleotide Sequencing, Lymphoma, T-Cell, Peripheral genetics, Mutation, Sequence Analysis, RNA, Exome Sequencing
- Abstract
Peripheral T-cell lymphoma not otherwise specified represents a diagnostic category comprising clinically, histologically, and molecularly heterogeneous neoplasms that are poorly understood. The genetic landscape of peripheral T-cell lymphoma not otherwise specified remains largely undefined, only a few sequencing studies having been conducted so far. In order to improve our understanding of the genetics of this neoplasm, we performed whole exome sequencing along with RNA-sequencing in a discovery set of 21 cases. According to whole exome sequencing results and mutations previously reported in other peripheral T-cell lymphomas, 137 genes were sequenced by a targeted deep approach in 71 tumor samples. In addition to epigenetic modifiers implicated in all subtypes of T-cell neoplasm (TET2, DNMT3A, KMT2D, KMT2C, SETD2), recurrent mutations of the FAT1 tumor suppressor gene were for the first time recorded in 39% of cases. Mutations of the tumor suppressor genes LATS1, STK3, ATM, TP53, and TP63 were also observed, although at a lower frequency. Patients with FAT1 mutations showed inferior overall survival compared to those with wild-type FAT1. Although peripheral T-cell lymphoma not otherwise specified remains a broad category also on molecular grounds, the present study highlights that FAT1 mutations occur in a significant proportion of cases, being provided with both pathogenetic and prognostic impact.
- Published
- 2020
- Full Text
- View/download PDF
46. Recurrent PDL1 expression and PDL1 (CD274) copy number alterations in breast implant-associated anaplastic large cell lymphomas.
- Author
-
Tabanelli V, Corsini C, Fiori S, Agostinelli C, Calleri A, Orecchioni S, Melle F, Motta G, Rotili A, Di Napoli A, and Pileri SA
- Subjects
- Adult, Aged, B7-H1 Antigen metabolism, Biomarkers, Tumor genetics, Breast Neoplasms etiology, Breast Neoplasms pathology, Female, Humans, Immunohistochemistry, Lymphoma, Large-Cell, Anaplastic etiology, Lymphoma, Large-Cell, Anaplastic pathology, Middle Aged, Phosphorylation, STAT3 Transcription Factor metabolism, B7-H1 Antigen genetics, Breast Implants adverse effects, Breast Neoplasms genetics, DNA Copy Number Variations, Lymphoma, Large-Cell, Anaplastic genetics
- Abstract
Breast implant-associated anaplastic large cell lymphoma (BI-ALCL) is a variant of anaplastic large cell lymphoma arising within seroma effusion associated with breast implants. BI-ALCL is a rare disease, recently recognized as a new provisional entity by the 2017 revised World Health Organization classification. All BI-ALCLs tested so far showed a "triple-negative" genetic profile-negative for ALK, DUSP22, and TP63 rearrangements-and were characterized by mutational and gene expression profiles consistent with aberrant activation of the JAK/STAT pathway. The active form of STAT3 (pSTAT3) is constantly expressed in BI-ALCLs and may favor tumor immune escape by triggering the transcription of PDL1 (CD274), a gene encoding the immune-checkpoint molecule programmed cell death ligand 1 (PDL1); immunohistochemical positivity for PDL1 has been recently described in 3 BI-ALCL cases, and one of them also harbored PDL1 gene amplification. We evaluated PDL1 and pSTAT expression by immunohistochemistry and PDL1 copy number alterations (CNAs) at chromosome 9p24.1 by fluorescent in situ hybridization in a cohort of 9 BI-ALCL cases; we also investigated the presence of tumor-infiltrating programmed cell death 1 (PD1)+ T cells (tumor-infiltrating lymphocytes, or TILs) and PDL1+ tumor-associated macrophages (TAMs) in BI-ALCL microenvironment. Tumor cells expressed PDL1 in 5 (56%) of 9 cases and harbored PDL1 CNAs in 3 (33%) of 9 cases; immunohistochemistry for pSTAT3 was positive in all 6 cases tested (100%), indicative of active JAK/STAT signaling. We observed PDL1 CNAs only among PDL1-positive cases, whereas PD1+ TILs and PDL1+ TAMs were present at variable levels in both PDL1-positive and PDL1-negative BI-ALCLs. We report frequent PDL1 expression and recurrent PDL1 CNAs in BI-ALCLs: our data suggest that 9p24.1 alterations represent a common mechanism of PDL1 overexpression in this disease, likely acting in synergy with constitutive pSTAT3 signaling. In PDL1-positive cases without chromosomal aberration, PDL1 expression may be induced by JAK/STAT signaling alone and/or others alternative pathways. BI-ALCL microenvironment hosts variable amounts of PD1+ TILs and PDL1+ TAMs, suggesting the presence of an active PD1/PDL1 axis. These findings may be of therapeutic value in advanced-stage patients who may benefit from a PD1/PDL1 blocking treatment., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
47. Blastic Plasmacytoid Dendritic Cell Neoplasm: State of the Art and Prospects.
- Author
-
Sapienza MR, Pileri A, Derenzini E, Melle F, Motta G, Fiori S, Calleri A, Pimpinelli N, Tabanelli V, and Pileri S
- Abstract
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is an extremely rare tumour, which usually affects elderly males and presents in the skin with frequent involvement of the bone-marrow, peripheral blood and lymph nodes. It has a dismal prognosis, with most patients dying within one year when treated by conventional chemotherapies. The diagnosis is challenging, since neoplastic cells can resemble lymphoblasts or small immunoblasts, and require the use of a large panel of antibodies, including those against CD4, CD56, CD123, CD303, TCL1, and TCF4. The morphologic and in part phenotypic ambiguity explains the uncertainties as to the histogenesis of the neoplasm that led to the use of various denominations. Recently, a series of molecular studies based on karyotyping, gene expression profiling, and next generation sequencing, have largely unveiled the pathobiology of the tumour and proposed the potentially beneficial use of new drugs. The latter include SL-401, anti-CD123 immunotherapies, venetoclax, BET-inhibitors, and demethylating agents. The epidemiologic, clinical, diagnostic, molecular, and therapeutic features of BPDCN are thoroughly revised in order to contribute to an up-to-date approach to this tumour that has remained an orphan disease for too long.
- Published
- 2019
- Full Text
- View/download PDF
48. Blastic plasmacytoid dendritic cell neoplasm: genomics mark epigenetic dysregulation as a primary therapeutic target.
- Author
-
Sapienza MR, Abate F, Melle F, Orecchioni S, Fuligni F, Etebari M, Tabanelli V, Laginestra MA, Pileri A, Motta G, Rossi M, Agostinelli C, Sabattini E, Pimpinelli N, Truni M, Falini B, Cerroni L, Talarico G, Piccioni R, Amente S, Indio V, Tarantino G, Brundu F, Paulli M, Berti E, Facchetti F, Dellino GI, Bertolini F, Tripodo C, Rabadan R, and Pileri SA
- Subjects
- Aged, Animals, Cell Line, Tumor, Female, Humans, Male, Mice, Mice, Inbred NOD, Mice, SCID, Middle Aged, Xenograft Model Antitumor Assays, Azacitidine pharmacology, Decitabine pharmacology, Epigenesis, Genetic drug effects, Gene Expression Regulation, Neoplastic drug effects, Hematologic Neoplasms drug therapy, Hematologic Neoplasms genetics, Hematologic Neoplasms metabolism, Hematologic Neoplasms pathology, Myeloproliferative Disorders drug therapy, Myeloproliferative Disorders genetics, Myeloproliferative Disorders metabolism, Myeloproliferative Disorders pathology, Neoplasm Proteins biosynthesis, Neoplasm Proteins genetics, Skin Neoplasms drug therapy, Skin Neoplasms genetics, Skin Neoplasms metabolism
- Abstract
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and aggressive hematologic malignancy for which there is still no effective therapy. In order to identify genetic alterations useful for a new treatment design, we used whole-exome sequencing to analyze 14 BPDCN patients and the patient-derived CAL-1 cell line. The functional enrichment analysis of mutational data reported the epigenetic regulatory program to be the most significantly undermined ( P <0.0001). In particular, twenty-five epigenetic modifiers were found mutated (e.g. ASXL1, TET2, SUZ12, ARID1A, PHF2, CHD8); ASXL1 was the most frequently affected (28.6% of cases). To evaluate the impact of the identified epigenetic mutations at the gene-expression and Histone H3 lysine 27 trimethylation/acetylation levels, we performed additional RNA and pathology tissue-chromatin immunoprecipitation sequencing experiments. The patients displayed enrichment in gene signatures regulated by methylation and modifiable by decitabine administration, shared common H3K27-acetylated regions, and had a set of cell-cycle genes aberrantly up-regulated and marked by promoter acetylation. Collectively, the integration of sequencing data showed the potential of a therapy based on epigenetic agents. Through the adoption of a preclinical BPDCN mouse model, established by CAL-1 cell line xenografting, we demonstrated the efficacy of the combination of the epigenetic drugs 5'-azacytidine and decitabine in controlling disease progression in vivo ., (Copyright© 2019 Ferrata Storti Foundation.)
- Published
- 2019
- Full Text
- View/download PDF
49. Dissecting diffuse large B-cell lymphomas of the "not otherwise specified" type: the impact of molecular techniques.
- Author
-
Pileri SA, Derenzini E, Melle F, Motta G, Calleri A, Antoniotti P, Maltoni V, Spagnolo S, Fiori S, Tabanelli V, and Fabbri M
- Subjects
- Animals, DNA Copy Number Variations, Humans, Sequence Analysis, DNA, Transcriptome, Lymphoma, Large B-Cell, Diffuse classification, Precision Medicine methods, Translational Research, Biomedical methods
- Abstract
The updated edition of the Classification of Tumours of Haematopoietic and Lymphoid Tissues, published in September 2017 by the World Health Organization (WHO), presents many important changes to the document published in 2008. Most of these novelties are linked to the exceptional development of biomolecular techniques during the last 10 years. To illustrate how much new technologies have contributed to the better classification of single entities, as well as the discovery of new ones, would go beyond the objectives of this work. For this reason, we will take diffuse large B-cell lymphoma as an example of the cognitive improvement produced by high-yield technologies (such as the gene expression profile, the study of copy number variation, and the definition of the mutational spectrum). The acquisition of this knowledge not only has a speculative value but also represents the elements for effective application in daily practice. On the one hand, it would allow the development of personalised therapy programs, and on the other it would promote the transition from the bench of the researcher's laboratory to the patient's bedside., Competing Interests: No competing interests were disclosed.No competing interests were disclosed.No competing interests were disclosed.
- Published
- 2018
- Full Text
- View/download PDF
50. Lymphomatoid granulomatosis and large granular lymphocyte leukemia, a rare association of two lymphoproliferative disorders.
- Author
-
De Luca G, Trasarti S, Bizzoni L, Del Giudice I, Della Starza I, De Propris MS, Gentile G, Mancini F, Mantovani S, Petrucci L, Tabanelli V, Guarini A, Vignetti M, and Foà R
- Subjects
- Diagnosis, Differential, Humans, Lung Diseases diagnosis, Male, Middle Aged, Leukemia, Large Granular Lymphocytic diagnosis, Lung pathology, Lymphomatoid Granulomatosis diagnosis, Lymphoproliferative Disorders diagnosis
- Published
- 2018
- Full Text
- View/download PDF
Catalog
Books, media, physical & digital resourcesDiscovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.