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BPR0C261 is a synthetic small molecule compound cytotoxic against human cancer cells and active prolonging the lifespan of leukemia mice. In the present study, we further investigated the mechanisms of its anticancer action and found that BPR0C261 inhibited microtubule polymerization through interacting with the colchicine binding sites on tubulins, disrupted microtubule arrangement and caused cell cycle arrest at G(2)/M phase in cancer cells. BPR0C261 also inhibited the clonogenic growths of cancer cells and showed cytotoxicity against human cervical cancer cells of multidrug-resistant phenotype. In addition, BPR0C261 concentration-dependently inhibited the proliferation and migration of HUVECs and disrupted the endothelial capillary-like tube formations in HUVEC and rat aorta ring cultures. Given orally, BPR0C261 inhibited angiogenesis in s.c. implanted Matrigel plugs in mice. Notably, its IC(50) values against the endothelial cell growths were approximately 10-fold lower than those against the cancer cells. It was found orally absorbable in mice and showed a good oral bioavailability (43%) in dogs. BPR0C261 permeated through the human intestinal Caco-2 cell monolayer, suggesting oral availability in humans. Orally absorbed BPR0C261 distributed readily into the s.c. xenografted tumors in nude mice in which the tumor tissue levels of BPR0C261 were found oral dose-dependent. BPR0C261 showed in vivo activities against human colorectal, gastric, and nasopharyngeal tumors in nude mice. Most interestingly, the combination of BPR0C261 plus cisplatin synergistically prolonged the lifespans of mice inoculated with murine leukemia cells. Thus, BPR0C261 is a novel orally active tubulin-binding antitumor agent with antimitotic, apoptosis-inducing, and vasculature disrupting activities., (© 2010 Japanese Cancer Association.)

Background: Antimitotic tubulin-binding BPR0L075 is structurally analogous to the vascular-disrupting combretastatin A-4., Materials and Methods: In vitro/in vivo models of endothelial cells cultures, Matrigel plug assay, tumor-bearing nude mice, and murine leukemia cells-inoculated mice were utilized to evaluate BPR0L075 for antiangiogenic and antitumoral activity spectra., Results: BPR0L075 concentration-dependently inhibited proliferation and migration of human umbilical vein endothelial cells (HUVECs), disrupted capillary tube formations of HUVECs and rat aorta endothelial cells, and suppressed in vivo VEGF-mediated angiogenesis in Matrigel plugs in mice. Besides inhibiting the colony growth of cancer cells, BPR0L075 suppressed growth of subcutaneously-xenografted human lung, colorectal, and cervical solid tumors in nude mice. Combination treatments of BPR0L075 plus cisplatin, compared to either agent alone, demonstrated a stronger growth inhibition against the tumor xenografts in nude mice and longer lifespan in the leukemia mice., Conclusion: BPR0L075 is an antitumoral and antiangiogenic agent and potentiates the anticancer activity of cisplatin.

Statement of Problem: Characterizing and reproducing color remain one of the most challenging aspects of dentistry. A relatively new intraoral colorimeter measures the color of natural teeth and metal-ceramic restorations and prints out a color recipe for the Vintage Halo Porcelain System. The reliability of the colorimeter is unknown., Purpose: The purpose of this study was to evaluate the reliability of a contact dental colorimeter and to correlate the shade registered by the colorimeter with the shade selected by experienced clinicians., Materials and Methods: In part I of the study, 2 examiners (A and B) took 2 colorimeter measurements from the maxillary right central incisors of 11 subjects. The examiners were blinded to their own data and those of other investigators. The readings were repeated 3 weeks later with the same protocol. The Cronbach alpha reliability coefficient was used to analyze the collected data. In part II of the study, 2 experienced clinicians (examiners D and E) selected a shade from the classic Vita Lumin Vacuum shade guide for the maxillary right central incisors of the same 11 subjects. The clinicians were blinded to each other's selections and the colorimeter readings. It should be noted that the manufacturer of the colorimeter uses the terms shade, value, and hue to represent chroma, value, and hue, respectively, as defined in the Glossary of Prosthodontic Terms (J Prosthet Dent 1999;81:39-110)., Results: The reliability analysis results for each of the combined trials for shade, value, and hue were all >.94. The interexaminer reliability alpha values were >.9 for shade and value and.64 to.74 for hue. The interexaminer alpha represented the value range of each of 4 measurements. The intraexaminer reliability alpha values for shade, value, and hue were.99,.95, and.96 for examiner A and.99,.93, and.97 for examiner B, respectively. In part II of the study, the colorimeter agreed with itself 82% of the time, whereas clinicians agreed with each other on the selected shade 73% of the time. Selections made by the colorimeter and the clinicians matched 55% to 64% of the time., Conclusions: Within the limitations of this study, the colorimeter reliably measured the color of natural teeth.

The purpose of this study was to determine and compare the antimicrobial action of a urea-based handwash lotion with triclosan with a lipid-depleting detergent-based handwash product with 4% chlorhexidine gluconate. The zones of inhibition produced by the two handwash lotions and the one produced by the positive control were similar against all strains of bacteria tested, indicating that a urea-based handwash lotion with triclosan is as effective as a lipid-depleting handwash lotion in inhibiting bacterial activity.

This article describes the modification of a procedure for fabricating a laboratory-processed, metal-reinforced, acrylic resin provisional restoration that becomes an implant-retained fixed partial denture. The modification involves the incorporation of patrix and matrix components into a cast metal framework. The prosthesis can be used as an alternative to a removable radiologic stent and surgical guide. It can function as a surgical guide during implant placement and help retract the buccal mucogingival flap during implant placement. The prosthesis also can be used as an aid in locating the implant during stage II surgery. Finally, the pontics can be converted into an implant-supported provisional restoration immediately after the implant prosthetic components are attached to the uncovered implants.

The aim of this study was to evaluate the in vitro microleakage of a condensable composite resin recommended for Class II restorations with and without a flowable composite resin as a liner. There were statistically significant differences between all groups and between individual groups (p < 0.05). The results showed significantly less microleakage in teeth restored with flowable composite resin underneath a condensable composite resin.

Objective: The purpose of this investigation was to evaluate the microleakage of Class V lesions restored with a compomer material (Dyractflow) using 2 single-bottle bonding systems., Method and Materials: Sixty-four Class V cavity preparations were made on 32 sound, extracted human premolars, 1 preparation on the facial surface and 1 on the lingual surface. Teeth were then randomly divided into 2 groups. Group 1, which served as the control group, was restored as follows: group 1a, facial surface with PQ1 single-bottle bonding system plus TPH resin composite; group 1b, lingual surface with Prime & Bond NT single-bottle bonding system plus TPH. Group 2 was restored as follows: group 2a, facial surface with PQ1 plus Dyractflow compomer material; group 2b, lingual surface with Prime & Bond NT plus Dyractflow. After 24 hours of storage in water and subsequent thermocycling, each specimen was immersed in 0.2% basic fuchsin dye for 24 hours. The teeth were sectioned longitudinally and horizontally, and dye penetration at enamel and cementum margins was viewed at x30 magnification by 2 independently calibrated evaluators. Specimens were evaluated as either having leaked or not having leaked., Results: No statistically significant differences in microleakage were noted between group 1 and group 2 specimens., Conclusion: Both single-bottle bonding systems, PQ1 and Prime & Bond NT, demonstrated equal effectiveness in reducing microleakage beneath compomer and composite restorations.

Objective: The purpose of this investigation was to evaluate microleakage in Class II cavities restored with the condensable resin composite SureFil, placed with or without a liner and 1 of 2 different dentin bonding agents., Method and Materials: Extracted noncarious human teeth were randomly divided into 4 groups of 15 each and restored according to manufacturer's instructions: group 1, SureFil and Prime & Bond NT; group 2, SureFil and PQ1; group 3, SureFil, PermaFlow, and PQ1; group 4, Heliomolar and Syntac Single-Component (control). Specimens were stored in 37 degrees C water for 24 hours. All specimens were thermocycled between 5 degrees C and 55 degrees C for 500 cycles. Each specimen was immersed in 0.2% basic fuchsin dye for 24 hours and then sectioned longitudinally and horizontally. Dye penetration at enamel and cementum margins were viewed at x30 magnification by 2 independent, calibrated evaluators. Specimens exhibiting dye penetration > or = 0.1 mm beyond the cavosurface margin were considered to have leakage., Results: Chi-square tests revealed no statistically significant differences in the presence of microleakage when all groups were analyzed together. However, 2 by 2 comparisons revealed that group 3 had significantly fewer specimens with microleakage than did group 1, 2, or 4., Conclusion: To limit microleakage, a flowable composite should be used as a liner when a condensable composite material is used.

This article describes a procedure that simultaneously registers maximal intercuspal position and gingival emergence profile. The materials used in this procedure are inexpensive, readily available, and easy to use. The clinician can inspect the framework, cast, and tissue profile before sending them to the laboratory. When this method is properly used, it allows better communication with the dental technician, saves chair time, and reduces the number of laboratory procedures as currently practiced.

Statement of Problem: Most microleakage studies have used low molecular weight dyes or isotopes rather than clinically relevant materials, such as lipopolysaccharides or cell wall materials, that have been shown to provoke inflammatory reactions in the dental pulp., Purpose: This study evaluated the leakage (diffusion) of lipopolysaccharide and dextran beneath cast gold crowns luted with 3 cements., Materials and Methods: Thirty extracted molars were prepared for crowns. Ten crowns with access ports (facial or lingual) were cast in gold and luted with zinc phosphate, glass ionomer, and an adhesive resin cement onto their preparations. Teeth and crowns with filters inserted into the ports were immersed in a solution of labeled macromolecules (TRITC-LPS, FITC-dextran) and evaluated for leakage at 2 weeks, and 1, 2, 3, 4, 5, and 6 months. Filters were retrieved and analyzed with fluorescent microscopy., Results: All filters retrieved from crowns luted with zinc phosphate, glass ionomer, and adhesive resin cements demonstrated no detectable leakage and were negative for both FITC-dextran and TRITC-lipopolysaccharide at all evaluation periods., Conclusion: Leakage of lipopolysaccharides and dextran did not occur during the period of this study. For the length of this investigation, zinc phosphate, Ketac-Cem, and C&B-Metabond were equally effective at preventing leakage of detectable molecular concentrations of lipopolysaccharide and dextran to the level of the access ports.

Two acellular pertussis vaccines, one containing only LPF toxoid (25 micrograms) and the other containing LPF toxoid (25 micrograms) and FHA (25 micrograms) and each combined with diphtheria and tetanus toxoids, were evaluated in two groups of 25 infants. A third group of 25 infants served as controls and received a DTP whole-cell pertussis vaccine. Infants given either acellular pertussis vaccine had significantly fewer local and systemic reactions than infants given whole-cell vaccine. Among the three vaccine groups, infants given the LPF vaccine (single component) had the highest concentration of antibody to LPF after three immunizations. Infants receiving the LPF/FHA vaccine (two-component) had the highest concentration of antibody to FHA after three immunizations. Infants vaccinated with the two-component vaccine had a significantly lower serological response to LPF than infants given the single component vaccine, as measured by either enzyme-linked immunosorbent assay or CHO cell assay. Further studies are necessary to determine why differences in immunogenicity of the two investigational vaccines occurred.