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The ubiquitin-proteasome system (UPS) plays a pivotal role in regulating protein homeostasis and cellular processes, including protein degradation, trafficking, DNA repair, and cell signaling. During cerebral ischemia, ischemic conditions profoundly disrupt UPS activity, leading to proteasomal dysfunction and the accumulation of abnormal proteins. This imbalance contributes to neuronal injury and cell death observed in ischemic stroke. The UPS is intricately linked to various signaling pathways crucial for neuronal survival, inflammation, and cellular stress response, such as NF-κB, TRIM, TRIP, JAK-STAT, PI3K/Akt, and ERK1/2. Alterations in the ubiquitination process can significantly impact the activation and regulation of these pathways, exacerbating ischemic brain injury. Therapeutic approaches targeting the UPS in cerebral ischemia aim to rebalance protein levels, reduce proteotoxic stress, and mitigate neuronal injury. Strategies include proteasome inhibition, targeting specific ubiquitin ligases and deubiquitinating enzymes, and modulating ubiquitination-mediated regulation of key signaling pathways implicated in ischemia-induced pathophysiology. Therefore, the present review discusses the molecular mechanisms underlying UPS dysfunction in ischemic stroke is crucial for developing effective therapeutic interventions. Modulating ubiquitination-mediated pathways through therapeutic interventions targeting specific UPS components holds significant promise for mitigating ischemic brain injury and promoting neuroprotection and functional recovery in patients with cerebral ischemia. [ABSTRACT FROM AUTHOR]

Abstract We used a neuromusculoskeletal model of bipedal walking to examine the effects of foot–ground friction conditions and gait patterns on slip- and trip-induced falls. We developed three two-dimensional neuro-musculoskeletal models in a self-organized manner representing young adults, elderly non-fallers, and elderly fallers. We simulated walking under different foot-ground friction conditions. The static friction coefficient between the foot and the ground was varied from 0.05 to 2.0. Under low friction conditions, the three gait models demonstrated slip-induced falls. The elderly faller model experienced the most slip. This is because the RCOF was higher in the elderly faller model due to its short stride length but much smaller foot clearance. Under high friction conditions, only the elderly faller model demonstrated trip-induced falls. Based on the analysis using the margin of stability, the forward postural stability of the model gradually decreased under high-friction conditions, with the toe of the swing foot contacting the ground and subsequently falling forward. These results imply that there is an optimal coefficient of friction for the ground to prevent slip- and trip-induced falls by people with less stable gaits, which may provide new insights into the design of shoes and floor surfaces for the elderly.

In this work, we investigate the microstructures and mechanical properties evolution on a novel carbon-based maraging steel with the compositions of Fe–10Mn-0.25C–1Cr–2Mo wt%, through hot and cold rolling following by heat treatment. The composition was designed through thermodynamic modelling to achieve a combination of high strength and high tensile elongation through the development of fine tempered martensite with carbide strengthening, and the transformation induced plasticity (TRIP) from the reverted austenite. After hot and cold deformation, a solution treatment at 870 °C followed by quenching gave a martensitic structure with 5% retained austenite. The microstructure and tensile properties were then investigated in detail as a function of ageing time at 510 °C. The kinetics of austenite reversion were much more rapid, with a peak in austenite content after only 5h, giving a maximum volume fraction of ∼26%, with a sharp reduction for longer ageing times. The best tensile properties were obtained after an 8 h age with only ∼16.5% of reverted austenite, which provided a UTS of 1560 MPa (220 MPa contributed by TRIP) and an elongation of 14.5%. While the smallest grain size was attained after 16 h of aging, accompanied by an exceedingly low volume fraction of reverted austenite (∼2.1%) for the TRIP effect, the UTS decreased to 1356 MPa, with a slightly improved elongation of 15.7%. The results show that achieving the optimal mechanical properties requires balancing the volume fraction of reverted austenite for the TRIP effect with the grain size to enhance both strength and ductility.

Finite element models of the burnishing process for galvanized Advanced High-Strength Steels (AHSS) sheets were developed. To reduce the number of points that describe a real surface profile, optimization algorithms were implemented, considering three sequential stages: (1) surface profile filter, (2) mean line determination and, (3) eight roughness parameters validation. The finite element models developed accurately reproduce the experimental roughness profile, obtained with different burnishing forces. Also, the effect of the real surface roughness on the residual stress distribution was numerically investigated by considering the interaction between the coating layer present on the steel. The simulation showed that considering the real surface strongly influences the residual stress profile on the surface and to a certain depth.

Goal: The experimental study of the stumble phenomena is essential to develop novel technological solutions to limit harmful effects in at-risk populations. A versatile platform to deliver realistic and unanticipated tripping perturbations, controllable in their strength and timing, would be beneficial for this field of study. Methods: We built a modular tripping-eliciting system based on multiple compliant trip blocks that deliver unanticipated tripping perturbations. The system was validated with a study with 9 healthy subjects. Results: The system delivered 33 out of 34 perturbations (a minimum of 3 per subject) during the desired gait phase, and 31 effectively induced a tripping event. The recovery strategies adopted after the perturbations were qualitatively consistent with the literature. The analysis of the inertial motion unit signals and the questionnaires suggests a limited adaptation to the perturbation throughout experiments. Conclusions: The platform succeeded in providing realistic trip perturbations, concurrently limiting subjects’ adaptation. The presence of multiple compliant obstacles, tunable regarding position and perturbation strength, represents a novelty in the field, allowing the study of stumbling phenomena caused by obstacles with different levels of sturdiness. The overall system is modular and can be easily adapted for different applications.

This paper examines the issues of studying decision support systems for passenger travel in terms of identifying patterns of such behavior, their classification and clustering. A review of the literature of domestic and foreign authors concerning the analysis of patterns of transport behavior of the population and their recognition is given. The relevance of studying the transport behavior of the population using the theory of patterns is substantiated. An approach to describing patterns of behavior from the point of view of the theory of macrosystems has been formed. Examples of mobile applications for route planning, modern models for studying transport behavior based on BigData technologies, neural networks, and parsing theory are given. The problem of formalizing the description of transport behavior has been solved in order to obtain a tool for analyzing the influence of control actions on transport behavior. The conclusion is made about the need for a generalized representation of transport behavior, and, consequently, its pattern, in the form of logical models. The purpose of the study is to compile a generalized representation of transport behavior and its patterns within the framework of the theory of macrosystems by compiling a digital pattern that can be displayed as a set of graphical schemes and as a subsequent logical description. The results obtained consist in a detailed review of the literature on the topic of decision support systems when making a trip, determining patterns of passenger behavior, and the influence of individual behavior on the behavior of the entire transport system as a whole. A toolkit for determining patterns based on the theory of macrosystems has been developed. The analysis of various methods of data processing and visualization of results used by researchers is carried out, which additionally serves to determine the possibilities of qualitative and quantitative description of transport behavior. At the same time, the set of states of the elements of the transport system, specific measurable and/or calculated values, their relation to the micro- or macro-levels of the description of the transport system were determined. It is determined that the most convenient and comprehensive tool for representing and studying patterns is the theory of transport macrosystems.

Abstract Background Falls due to stumbling are prevalent for transfemoral prosthesis users and may lead to increased injury risk. This preliminary case series analyzes the transfemoral prosthesis user stumble recovery response to highlight key deficits in current commercially-available prostheses and proposes potential interventions to improve recovery outcomes. Methods Six transfemoral prosthesis users were perturbed on their prosthetic limb at least three times while walking on a treadmill using obstacle perturbations in early, mid and late swing. Kinematic data were collected to characterize the response, while fall rate and key kinematic recovery metrics were used to assess the quality of recovery and highlight functional deficits in current commercially-available prostheses. Results Across all participants, 13 (54%) of the 24 trials resulted in a fall (defined as > 50% body-weight support) with all but one participant (83%) falling at least once and two participants (33%) falling every time. In contrast, in a previous study of seven young, unimpaired, non-prosthesis users using the same experimental apparatus, no falls occurred across 190 trials. For the transfemoral prosthesis users, early swing had the highest rate of falling at 64%, followed by mid-swing at 57%, and then late swing at 33%. The trend in falls was mirrored by the kinematic recovery metrics (peak trunk angle, peak trunk angular velocity, forward reach of the perturbed limb, and knee angle at ground contact). In early swing all four metrics were deficient compared to non-prosthesis user controls. In mid swing, all but trunk angular velocity were deficient. In late swing only forward reach was deficient. Conclusion Based on the stumble recovery responses, four potential deficiencies were identified in the response of the knee prostheses: (1) insufficient resistance to stance knee flexion upon ground contact; (2) insufficient swing extension after a perturbation; (3) difficulty initiating swing flexion following a perturbation; and (4) excessive impedance against swing flexion in early swing preventing the potential utilization of the elevating strategy. Each of these issues can potentially be addressed by mechanical or mechatronic changes to prosthetic design to improve quality of recovery and reduce the likelihood a fall.

The present work investigates the influence of Al content on δ-ferrite formation and its volume fraction, resulting in different austenite stability, microstructure evolution, corresponding mechanical behaviour and underlying deformation mechanisms for three Fe-8Mn-XAl-1Si-0.2C steels with Al contents of 1.23 wt.% (1.25Al steel), 3.5 wt.% (3.5Al steel) and 5.1 wt.% (5Al steel), respectively. Steel ingots are casted and processed through hot deformation, entailing hot forging, hot rolling and subsequent intercritical annealing at various temperatures. It is observed that Al addition promotes the formation of δ-ferrite that enhances the mechanical stability of austenite. Diffusion simulation using TC-DICTRA supports that preferential partitioning of Mn and C from δ-ferrite to austenite leads to enhanced mechanical stability of austenite. Both maximum (k ∼ 0.49) and minimum (k ∼ 7.30) austenite stability, where obtained in 5Al and 1.25Al steels, respectively, followed by inferior mechanical properties. This occurs due to the presence of a higher δ-ferrite (∼ 65%) and no δ-ferrite fraction in 5Al and 1.25Al steels, respectively. However, achieving an intermediate δ-ferrite content of approximately 34% results in optimal stability of austenite (k ∼ 2.50) in 3.5Al steel that results in an optimal microstructure comprising both intercritical α-ferrite and δ-ferrite, as well as excellent tensile properties as represented by an ultimate tensile strength of 825 MPa with ∼18% elongation. [ABSTRACT FROM AUTHOR]

The mechanical properties of ausferritic ductile iron are strongly influenced by the ability of the austenite to undergo martensite formation. Strain-induced martensite formation occurring under the right circumstances results in transformation induced plasticity (TRIP) that improves ductility and strength. TRIP has been shown to depend on the austenite's stacking fault energy (SFE), which describes the crystals micromechanical behaviour. In austenitic FeMnAlSi-TRIP steels, TRIP as a reaction to mechanical load only occurs for SFE <20 mJ/m2. For carbon-stabilised austenite as in ADI, the relationship between stacking faults, mechanical properties and martensitic transformation has not yet been established. To investigate the TRIP-effect in ADI, unalloyed ADI with 3.43 wt% C, 2.52 wt% Si and 0.21 wt% Mn was ausferritised and subjected to tensile tests at temperatures between −180 °C and 200 °C. The amount of martensite produced by thermal and mechanical activation, crystalline microstrain and stacking fault density were measured on deformed and undeformed regions of the specimen by XRD and the resulting SFE calculated. Between −70 and 20 °C, the elongation at fracture exceeded 10 % compared to below 2 % in the temperature range of −130 to −180 °C. At temperatures above 80 °C, elongation at fracture gradually decreased to 4.5 % at 200 °C. High sample ductility was associated with lower SFEs as low as 35 mJ/m2, indicating a correlation of stacking fault formation and strain-induced martensite formation in ADI. [ABSTRACT FROM AUTHOR]

Copyright of Magazine of Historical Studies & Archaeology is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Laser surface texturing is a promising technique for improving the surface properties of metals. In this study, a nanosecond pulsed laser was used to perform surface texturing on a metastable austenitic stainless steel AISI 301LN. The effects of the number of laser passes (from 1 to 1000 passes) on the surface morphology, microstructure, and mechanical properties were investigated. The results show that the depth of laser tracks increases with the number of passes due to thermal accumulation. Increasing the number of laser passes produces a rougher surface, but beyond a certain value, a smoother texture is observed. The amount of α'-martensite phase induced by the laser increases with the number of passes, producing augments in hardness. However, again, this trend changes after a certain number of laser passes, and hardening saturates and even decreases. Therefore, selecting the appropriate laser processing parameters and number of passes is essential to control the dimensions of laser tracks and thus the desired surface texture and also to achieve the level of hardening. [ABSTRACT FROM AUTHOR]

Fall injury and fatality claims and legal cases involving ordinary pedestrians as well as employees/contractors at work sites have increased dramatically over the course of the author's 43-year engineering career. As a result, forensic engineers are frequently being contacted by insurers and attorneys to analyze these incidents. The need is to determine probable cause(s) and ascertain as to whether location features were designed, constructed, installed, manufactured, and/or maintained in accordance with the standard of care, including requirements specified in applicable codes and standards. The proper contemporary analysis techniques for these incidents are addressed in this paper as well as what constitutes proper basis for establishing a standard of care for involved installations and/or equipment. It will also expand on and update information provided in approximately two dozen past NAFE papers on various aspects of fall incident analysis, most of which are more than 10 to 30 years old. [ABSTRACT FROM AUTHOR]

Understanding why falls during pregnancy occur at over 25% rate over gestation has clinical impacts on the health of pregnant individuals. Attention, proprioception, and perception of the environment are required to prevent trips and falls. This research aimed to understand how the changes to these neurocognitive processes control obstacle avoidance through gestation. Seventeen pregnant participants were tested five times in 6-week intervals. Participants walked an obstacle course (OC), and we analyzed the crossings over obstacles that were set to 10% of participants' body height. Participants also performed an attentional network test (ANT: performance of specific components of attention), an obstacle perception task (OP: ability to visually define an obstacle and translate that to a body posture), and a joint position sense task (JPS: ability to recognize and recreate a joint position from somatosensation). In the OC task, average leading and trailing foot crossing heights significantly reduced by 13% and 23% respectively, with no change in variation, between weeks 13 and 31 of pregnancy, indicating an increased risk of obstacle contact during this time. The variability in minimum leading foot distances from the obstacle was correlated with all three neurocognition tasks (ANT, OP, and JPS). Increased fall rates in the second and third trimesters of pregnancy may be driven by changes in attention, with additional contributions of joint position sense and environmental perception at various stages of gestation. The results imply that a holistic examination on an individual basis may be required to determine individual trip risk and appropriate safety modifications. [ABSTRACT FROM AUTHOR]

Malaria is caused by Plasmodium parasites that multiply inside host cells and can be lethal when P. falciparum is involved. We identified tRip as a membrane protein that facilitates the import of exogenous transfer RNA (tRNA) into the parasite. tRip encompasses a tRNA binding domain exposed on the parasite surface. We used the SELEX approach to isolate high-affinity and specific tRip-binding RNA motifs from a library of random 25 nucleotide-long sequences. In five rounds of combined negative and positive selections, an enriched pool of aptamers was obtained; sequencing revealed that they were all different in their primary sequence; only by comparing their structure predictions did most of the selected aptamers reveal a conserved 5-nucleotide motif sequence. We showed that the integral motif is essential for tRip-binding while the rest of the molecule can be significantly reduced or mutated as long as the motif is presented in a single-stranded region. Such RNA aptamers bind in place of the original tRNA substrate and act as an efficient competitor, suggesting that they can block tRip function and slow parasite development. [Display omitted] • tRip is a conserved protein located on the surface of Plasmodium parasites. • tRip is a promising target for the development of new antimalarial drugs. • RNA aptamers were selected to interact specifically with tRip. • Most of the selected aptamers share a 5-nucleotide single-stranded motif. • The selected aptamers compete with tRNAs for binding to tRip. [ABSTRACT FROM AUTHOR]

The variations of the microstructure and mechanical properties of medium-Mn steel after vanadium (V) microalloying with different contents were investigated. After a one-step intercritical annealing (IA) at 730 °C, the steel containing 0.04 wt.% of V exhibited excellent comprehensive properties. The steel maintained an ultimate tensile strength (UTS) of 1000 MPa while also exhibiting a total elongation (TEL) of 37% and a product of strength and plasticity (PSE) of 37.7 GPa%. V-microalloying improved the yield strength (YS) and UTS of the experimental steel by refining ferrite grains and precipitation strengthening, however, it deteriorated its plasticity, which is difficult to compensate for through grain refinement and due to the TRIP effect of retained austenite (RA). The largest amount of RA and the appropriate stability also make a significant contribution to the outstanding UTS of the steel containing 0.04 wt.% of V through the TRIP effect. However, the further increase of V content led to decreased RA content and stability, weakening the TRIP effect and resulting in a weaker strength ductility balance. [ABSTRACT FROM AUTHOR]

Successful recovery following a perturbation during walking depends on a quick well-coordinated response from the body. As such, lower limb joint power and work provide critical information characterizing the success of the recovery after a perturbation. Therefore, this study aimed to investigate lower-limb joint power and the relative contribution of each joint to the total leg work during the recovery following a trip-induced perturbation. Twenty-four young male volunteers walked at 1.1 m/s for 2 min, followed by two unexpected perturbations induced by rapidly decelerating the right belt of the split-belt treadmill. Joint moments and powers were calculated using an inverse dynamic approach. Joint work was found as the integral of joint power with respect to time. Statistical parametric mapping (SPM) and paired-sample t-tests were used to compare joint power and work between recovery and unperturbed steps. Compared to normal walking, recovery from the trip required a significant increase in both positive (+27 %, p < 0.05) and negative(+28 %,p < 0.05) leg work. During unperturbed walking, the ankle was the key contributor to both positive (ankle=50 %, hip=34 %, and knee=15 %) and negative (ankle=62 %, knee=32 %, and hip=6 %) leg work. During recovery, the knee eccentric work significantly increased (+83 %,p < 0.05) making it the main contributor to the negative leg work (knee=46 %, ankle=45 %, and hip=9 %). The hip positive work also increased during recovery (+62.7 %, p < 0.05), while ankle and the knee positive work remained unchanged. These findings highlight the importance of eccentric work of the knee, and concentric work of the hip joint during recovery from trip-induced perturbations. The additional mechanical demand of producing and absorbing more power during recovery is primarily imposed on the knee and hip, rather than the ankle. This new insight into the specific functions of lower-limb joints during recovery from trip-induced perturbations has important implications for the design of targeted fall prevention interventions. • Trip perturbations altered lower-limb joints contribution to leg work. • Knee joint played the most crucial role in absorbing energy during recovery. • The excessive demand of producing more power was mainly imposed on the hip joint. [ABSTRACT FROM AUTHOR]

The aim of this research is to determine the views of 6th-grade students regarding historical site trips organized within the context of out-of-school learning environments in social studies courses. The research was conducted with a case study, one of the qualitative research methods. The study group of the research consisted of 12 students attending the 6th grade in a secondary school affiliated with the Muş Provincial Directorate of National Education during the 2022-2023 academic year. In the research, “Open-Ended Questionnaire Form”, “Semi-Structured Interview Form”, and “Student Trip Diary” were used as data collection tools. The data obtained was evaluated through content analysis in the research. It was determined that students were willing to visit out-of-school learning environments such as historical sites or museums to gain knowledge, to see different places, and to learn lessons in these environments. It was concluded in the study that the school trips organized to out-of-school learning environments and the activities carried out during these trips provided benefits in terms of evoking emotions such as excitement, happiness, pride, and enjoyment in students. Furthermore, it was observed that the activities conducted during these visits to the historical sites were liked by students due to being both fun and educational. The research revealed that students’ level of knowledge about historical sites in their local environment increased. In addition, it was concluded that out-of-school learning environments contributed to students’ experiential learning, facilitating permanent and effective learning, and fostering students’ enjoyment of the subject. [ABSTRACT FROM AUTHOR]

The ride-sharing trend is booming because of modern technologies and plays a significant role in transitioning to sustainable transportation methods. This study utilizes a comprehensive dataset collected from an online survey administered to over 1300 individuals in Bangladesh in 2023. Its specific focus is to examine the usage patterns of ride-sharing applications, such as Pathao and Uber. It builds upon previous research to analyze the factors influencing the use of these services and current trends in shared transportation. Additionally, it investigates the challenges associated with utilizing shared transportation amenities and proposes potential improvements to make ride-sharing or pooling more attractive. The study diligently worked to establish an inclusive framework and terminology to enhance policies and gain a more detailed understanding of the transportation choices and requirements of communities. To bridge the gap between theory and practice, we adopted an implementation-oriented research approach and conducted a comprehensive quantitative and inferential analysis of the survey data. The inclination of an individual to opt for or perceive collective or merged services depends on several demographic, geographical, and built-environment factors. The findings highlight the necessity of enhanced security measures, increased public awareness of ride-sharing benefits, and the formulation of legislative measures to promote sustainable mobility. Two examples of potential measures we have found to improve and encourage the use of ride-sharing services include reducing uncertainty about shared rides and minimizing passenger discomfort. These measures can be implemented through operational adjustments, government regulations, and employer programs. An essential conclusion was reached regarding the importance of prioritizing improvements in ride-sharing operations, rather than relying solely on cash incentives to promote behavioral change. Furthermore, it was determined that regardless of the changes, policies, or programs implemented, it is imperative to enhance public awareness and education.

In this article, the authors consider the prerequisites for creating a single operator of urban passenger transportation. This organization is necessary to control the operation of all types of urban passenger transport. The prerequisites are problems in the field of urban passenger transport: poor quality of public transport services, which leads to numerous complaints and dissatisfaction of passengers. A single operator of urban passenger transportation will help solve these problems, as it will monitor the quality of services provided by carriers. The purpose: to analyze the prerequisites for creating a single operator of urban passenger transportation in modern cities. Method or methodology of the work: statistical analysis and synthesis were used in the article. Results: the problems of urban passenger transport are identified, which are prerequisites for the creation of a single operator of urban passenger transportation, namely, low indicators of the quality of passenger transport services according to various criteria Scope of application of the results: research activity on the development of new approaches in the field of organization of transportation and transport services.

CHO cells are most commonly used for the synthesis of recombinant proteins in biopharmaceutical production. When stable producer cell lines are obtained, the locus of transgene integration into the genome has a great influence on the level of its expression. Therefore, the identification of genomic loci ensuring a high level of protein production is very important. Here, we used the TRIP assay to study the influence of the local chromatin environment on the activity of transgenes in CHO cells. For this purpose, reporter constructs encoding eGFP under the control of four promoters were stably integrated into the genome of CHO cells using the piggyBac transposon. Each individual transgene contained a unique tag, a DNA barcode, and the resulting polyclonal cell population was cultured for almost a month without any selection. Next, using the high-throughput sequencing, genomic localizations of barcodes, as well as their abundances in the population and transcriptional activities were identified. In total, ~640 transgenes more or less evenly distributed across all chromosomes of CHO cells were characterized. More than half of the transgenes were completely silent. The most active transgenes were identified to be inserted in gene promoters and 5’ UTRs. Transgenes carrying Chinese hamster full-length promoter of the EF-1α gene showed the highest activity. Transgenes with a truncated version of the same promoter and with the mouse PGK gene promoter were on average 10 and 19 times less active, respectively. In total, combinations of genomic loci of CHO cells and transgene promoters that together provide different levels of transcriptional activity of the model reporter construct were described.

We report the annealing time-dependent microstructure–mechanical properties relationship of cold-rolled and annealed Fe–6Mn-0.05C–3Ni-1.5Al (wt.%) steel, containing Ni and Al to form NiAl B2 precipitates. The microstructures of the investigated materials after cold-rolling and intercritical annealing show a mixture of lath and equiaxed zones consisting of a triplex matrix phase (ferrite, austenite, and tempered martensite) with B2 precipitates. Recovery of the deformed martensite in the cold-rolled microstructure during annealing resulted in the formation of sub-boundaries and a continuous transition from partially-recrystallized tempered martensite to recrystallized ferrite. The investigated materials showed a decrease in strength and an increase in ductility and strain-hardening rate with increasing annealing time. A decrease in the fraction of tempered martensite, an increase in the fraction of recrystallized ferrite/austenite grains, and enhanced transformation-induced plasticity (TRIP) kinetics with increasing annealing time led to enhanced ductility and strain hardening of the materials. The mixed presence of less stable equiaxed austenite and more stable lath austenite resulted in sustained TRIP effect during tensile deformation and superior strain hardening capacity of the specimen annealed for 24 h. This study provides a novel microstructure design solution for medium Mn steels, and further optimization of the composition and processing will lead to the development of medium Mn steels with superior mechanical performance.

This article, published in Clinical Pediatric Endocrinology, discusses a case of adrenal crisis (AC) in a young child with septo-optic dysplasia (SOD) during a trip. The patient, a 16-month-old Japanese child with SOD and intellectual disability, developed AC after a long-distance trip. The authors suggest that prolonged periods in unfamiliar environments during trips may be a potential precipitating factor for AC in susceptible individuals. They recommend considering prophylactic administration of a stress dose of hydrocortisone during trips to prevent AC. However, further research is needed to determine the effectiveness of this approach. [Extracted from the article]

Introduction. The process of planning public transport is divided into a few number of tasks in strategic, tactical and operational levels, which include the development route system, the establishment of traffic intensity along routes, the design of the structure of road transport vehicles, the distribution of the current road transport vehicles along the routes, determining the required amount of the subsidies, etc. Materials and methods. The article presents a multi-criteria mathematical model of transportation design, according to which the tasks have been solved by distributing (limited or unlimited) transport resources between the permissible routes of the transport network in accordance with a variety of efficiency criteria, which can be the subject to appropriate restrictions. The formation acceptable routes on the transport network can be carried out by means of the algorithms developed, for example, in the framework of solving TNDP and TNDFSP tasks. The routes can also be adjusted by experts. Transport demand is set by a matrix of passenger correspondence generated for the entire period of traffic on a weekday, to ensure that all passenger flows along the route network are taken into account, and not only the periods of the greatest traffic intensity. The calculation of the parameters of the transport offer in the process of solving the problems of transportation design is carried out based on the results of the passenger correspondence distribution on the route network (PAP), which is a complex problem unsolved today. The article describes the developed PAP methodology based on a flexible passenger strategy that takes into account the waiting time for transport, the proximity of the route through an empirical model of dividing demand by the length of trips, the preferences of transport modes, the possible redistribution of passenger flows between the stopping points of the network located within a walking distance. Results. The tasks of designing public transport transportation have been formulated, a multi-criteria mathematical model of their solution has been developed. The method of distribution of passenger flows on the route network based on a flexible passenger strategy has been described. The effectiveness of the developed PAP methodology is shown on test calculations, which were carried out using passenger correspondence, an average weekday in October 2019, obtained by processing validations of e-tickets of urban passenger transport in Krasnoyarsk. Discussion and conclusion. The practical implementation of the described methodology for the distribution of passenger flows on the route network using relational database management systems (DBMS) MS SQL Server has been carried out.

This study proposes a machine-learning (ML) model combining ab initio calculations and an experimental dataset of 201 alloys (in addition to pure Ti) to predict the activated plasticity mechanisms in β-Ti alloys. This methodology is shown to be more efficient than the so-called Bo¯-Md¯ approach, achieving 82% prediction accuracy while the Bo-Md approach leads to 52% correct predictions on the same dataset. In addition, four new alloy compositions were produced to verify the model validity. Specific cases where the present model disagreed with the Bo-Md predictions were chosen to increase the benefits of the produced results. The plasticity mechanisms of the four alloys experimentally confirmed the validity of the ML model. This approach particularly helps the design of specific Ti alloys exhibiting a high work hardening rate owing to the simultaneous activations of the Transformation-Induced Plasticity (TRIP) and mechanical twinning (TWIP) effects. Indeed, the class corresponding to the combination of TRIP and TWIP effects reach a prediction accuracy of 88%.

Dilatometric experiments were conducted with the main purpose of measuring the transformation-induced coefficients of 13% chromium and 4% nickel, which are martensitic stainless steel base and filler materials used for hydraulic turbine manufacturing. To this end, a set of experiments was conducted in a quenching dilatometer equipped with loading capabilities. The measurement system was further improved by means of modified pushrods to allow for the use of specimens with geometries that are compliant with tensile test standards. This improvement allowed for the measurement of the materials' phases and respective yield strengths. The dataset was further used to determine the relationship between the applied external stress and the martensitic start temperature ( M s ) upon cooling. The TRIP coefficient's K values for both the S41500 steel and E410NiMo filler material were measured at 8.12 × 10 − 5 and 7.11 × 10 − 5 , respectively. Additionally, the solid phase transformation model parameters for both the austenitic and martensitic transformation of the filler material were measured. These parameters were then used to model austenitic-phase-transformation kinetics and martensite transformation, including transformation-induced plasticity effects. Good agreement was achieved between the calculation and the experiments. [ABSTRACT FROM AUTHOR]

Certain medium-manganese (6–12 wt% Mn) steels can be heat-treated to produce single-phase (austenite) or multi-phase (ferrite, austenite, bainite, martensite, and/or iron carbide) microstructures. With the proper amount of silicon and aluminum, both transformation-induced plasticity (TRIP) and/or twinning-induced plasticity (TWIP) can be the active deformation mechanism. In this study, two steel heats with compositions of Fe–7.53Mn–2.01Al–1.94Si–0.74C and Fe–7.71Mn–1.57Al–1.51Si–0.50C were investigated. The lower-carbon steel had a wider heat treating range but did not achieve as high of a hardness as the higher-carbon steel. Multi-phase microstructures were produced in both of these steels, and the multi-phase microstructures had higher yield strength compared to single-phase (austenite) microstructures. A high iron and manganese content skull adhered to the sides of the furnace after induction melting. This skull was difficult to remove from the furnace lining. [ABSTRACT FROM AUTHOR]

In applications where a combination of good strength and corrosion resistance is required, 17-4 precipitation hardenable (PH) stainless steel is a common material choice. This alloy is traditionally processed through a combination of casting, rolling, and machining. A variety of heat treatments are used to anneal and harden the material via precipitation strengthening. While additive manufacturing (AM) removes many geometric design constraints from these traditional forming processes, until recently, structures fabricated via laser powder bed fusion (L-PBF) were porous and contained undesirable columnar grain structures that contributed to unpredictable and anisotropic mechanical properties. Recent advances in L-PBF processing technology including improved gas flow, powder atomization, and print parameter optimization enable printing of high-quality 17-4 PH with properties that are comparable to traditionally processed material. However, it is yet to be determined whether these properties can be reliably reproduced across various machines and if machine-agnostic material property baselines can be established based on data available thus far. If baselines can be established, their implementation should allow for identification of machines that generate non-conforming material. In this work, we evaluate the consistency of mechanical properties in L-PBF 17-4 PH produced by six vendors with the ultimate goal of establishing mechanical property baselines, which is fundamental to modern qualification paradigms. We find non-conforming data from two vendors by examining anomalies in mechanical response (e.g., transformation induced plasticity effects) and demonstrate that typical sources of variation can be detected using qualification testing protocols. Ultimately, after standard solution annealing and heat treating, the microstructure and mechanical properties across vendors converged with very few, easily explainable exceptions. In particular, powder atomized in nitrogen promoted formation of retained austenite that lead to a yield point phenomenon in as-built conditions, and high surface roughness from as-built surfaces reduced the fatigue strength. However, with conventional post-processing heat treatments and surface polishing, AM 17-4 PH behaved comparably and consistently to conventionally processed material. [ABSTRACT FROM AUTHOR]

In this comprehensive study, the multifaceted impact of laser surface texturing (LST) on AISI 301LN stainless steel is explored. Changes in the microstructure, mechanical properties, and grain characteristics are examined. The dynamic relationship between Schmid factor evolution and plastic deformation in this stainless steel alloy is unveiled through the analysis of grain statistics and individual grain scrutiny. It is revealed that LST initiates the formation of strain-induced α'-martensite, grain refinement, and substantial hardness enhancements. Notably, an α'-martensite crystalline size of 2.05 Å is induced by LST. Furthermore, a 12% increase in tensile strength is observed after LST along with an 11% boost in yield strength. However, reductions of 19% in elongation to fracture and 12% in the area reduction are experienced. [ABSTRACT FROM AUTHOR]

This study evaluates the performance of a user-defined combined hardening modeling method for advanced high-strength steels (AHSS) under monotonic and reversal loading conditions. The plastic behavior of TWIP980 and TRIP980 AHSS sheet metals is investigated using a cyclic plasticity modeling approach. The model incorporates an isotropic von Mises yield criterion and a single-term Chaboche nonlinear kinematic hardening rule. Monotonic and reversal loading stress-strain curves are predicted and compared with experimental results. The model accurately captured the Bauschinger effect for both materials, but it needs help to effectively model the permanent softening behavior observed in TWIP980 steel. Overall, the proposed modeling method agrees well with experimental results for monotonic loading and accurately represents the Bauschinger effect and transient behavior during reversal loading. However, better improvements are needed to capture the permanent softening behavior of TWIP980 steel.

Copyright of Journal of Academic Social Science Studies is the property of Journal of Academic Social Science Studies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Iraqi Journal of Chemical & Petroleum Engineering is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The use of targeted integration for industrial CHO cell line development currently requires significant upfront effort to identify genomic loci capable of supporting multigram per liter therapeutic protein production from a limited number of transgene copies. To address this barrier to widespread adoption, we characterized transgene expression from thousands of stable hotspots in the CHO genome using the Thousands of Reporters Integrated in Parallel high‐throughput screening method. This genome‐scale data set was used to define a limited set of epigenetic properties of hotspot regions with sizes on the order of 10 kb. Cell lines with landing pad integrations at eight retargeted hotspot candidates consistently exhibited higher transgene mRNA expression than a commercially viable hotspot in equivalent culture conditions. Initial benchmarking of NISTmAb and trastuzumab productivity from one of these hotspots yielded mAb productivities of approximately 0.7–2 g/L (qP range: 2.9–8.2 pg/cell/day) in small‐scale fed‐batches. These findings indicate the list of hotspot candidates identified here will be a valuable resource for targeted integration platform development within the CHO community. [ABSTRACT FROM AUTHOR]

High strain hardening rate is usually pursued for high ductility. Here we report that moderate strain hardening rate is desired for producing better ductility and high yield strength, which is demonstrated in a medium carbon TRIP steel. By adjusting the temperature for bainitic transformation, the morphology, carbon content and residue stress of retained austenite were tuned to tailor the TRIP kinetics during tensile tests, which produced moderated but long-lasting strain hardening for high ductility while at the same time resulted in high yield strength. This strategy should work well for most materials, which usually have a limited ultimate strength.

The article investigates the purpose and process of a trip, which was conducted by Baron August von Haxthausen into the interior of the Russian Empire during 1843-1844. The scientific heritage of A. von Haxthausen was examined by various researchers, but most of them studied his works exclusively in the context of agricultural and socio-economic issues of Russia and Germany. However, the trip of A. von Haxthausen itself and his observations concerning ethnographic and religious peculiarities, social and cultural aspects of life of the local population were practically never explored before. The research was conducted based on two volumes of A. von Haxthausen's work «The Russian Empire, its People, Institutions, and Resources», with the primary focus being on his travelling through Ukrainian ethnic territories. In the course of the investigation, it was established that the purpose of Baron's trip was to explore the agricultural system of the Russian Empire, as well as receive further insight into different aspects of daily life, culture, and traditions of the local population. In addition, A. von Haxthausen demonstrated a great interest in various monuments, such as religious constructions, palaces, and educational institutions. His choice of the monuments was significantly influenced by what was popular among other travellers to visit in the late XVIII - early XIX centuries. Due to Baron's communication with people of different social classes certain changes in his attitude towards the imperial realities were induced, making it more objective and comprehensive. A. von Haxthausen was substantially interested in the living conditions of foreign colonists, particularly of Mennonites – his compatriots. While travelling, Baron clearly identified the ethnicity of each people of the Russian Empire. His ethnic classification was predominantly based on the language, physical features, and traditions of the population. All the data, which was collected by A. von Haxthausen in the course of the trip, including his observations and impressions, was later transformed into a three-volume work «The Russian Empire, its People, Institutions, and Resources», further laying the foundation of changes in the imperial society.

The socio-economic characteristics of individual and group have been found to significantly have an impact on their mobility and travel behaviour. Hence, this study examined the influence of socio-economic characteristics of households in the Rural areas of Nigeria on trip making. The study adopted the multi-stage sampling technique method where 12, 365 registered buildings were systematically selected out of a total of 28,341 buildings. Systematic sampling technique was used to survey 4 percent of households, representing 495 household heads of a total 12,365 registered buildings by means of questionnaire administration. The study revealed that majority of the respondents were between the age of 60–69 years and utilized the non-motorized (walk & bicycle) means of transport. The result of the multiple regression analysis further revealed that Age and Income were the significant socio-economic variables influencing trip making of households. This study thus advocates the influence of demographic attributes on households’ travel behaviour. Therefore, rural communities should be given equal privilege as urban areas when formulating transport policies as a result of their unique and distinct socio-economic attributes.

Trip perturbations are proposed to be a leading cause of falls in older adults. To prevent trip-falls, trip-related fall risk should be assessed and subsequent task-specific interventions improving recovery skills from forward balance loss should be provided to the individuals at risk of trip-fall. Therefore, this study aimed to develop trip-related fall risk prediction models from one's regular gait pattern using machine-learning approaches. A total of 298 older adults (≥60 years) who experienced a novel obstacle-induced trip perturbation in the laboratory were included in this study. Their trip outcomes were classified into three classes: no-falls (n = 192), falls with lowering strategy (L-fall, n = 84), and falls with elevating strategy (E-fall, n = 22). A total of 40 gait characteristics, which could potentially affect trip outcomes, were calculated in the regular walking trial before the trip trial. The top 50% of features (n = 20) were selected to train the prediction models using a relief-based feature selection algorithm, and an ensemble classification model was selected and trained with different numbers of features (1–20). A ten-times five-fold stratified method was utilized for cross-validation. Our results suggested that the trained models with different feature numbers showed an overall accuracy between 67% and 89% at the default cutoff and between 70% and 94% at the optimal cutoff. The prediction accuracy roughly increased along with the number of features. Among all the models, the one with 17 features could be considered the best model with the highest AUC of 0.96, and the model with 8 features could be considered the optimal model, which had a comparable AUC of 0.93 and fewer features. This study revealed that gait characteristics in regular walking could accurately predict the trip-related fall risk for healthy older adults, and the developed models could be a helpful assessment tool to identify the individuals at risk of trip-falls. [ABSTRACT FROM AUTHOR]

The variations of the microstructure and mechanical properties of medium-Mn steel after vanadium (V) microalloying with different contents were investigated. After a one-step intercritical annealing (IA) at 730 °C, the steel containing 0.04 wt.% of V exhibited excellent comprehensive properties. The steel maintained an ultimate tensile strength (UTS) of 1000 MPa while also exhibiting a total elongation (TEL) of 37% and a product of strength and plasticity (PSE) of 37.7 GPa%. V-microalloying improved the yield strength (YS) and UTS of the experimental steel by refining ferrite grains and precipitation strengthening, however, it deteriorated its plasticity, which is difficult to compensate for through grain refinement and due to the TRIP effect of retained austenite (RA). The largest amount of RA and the appropriate stability also make a significant contribution to the outstanding UTS of the steel containing 0.04 wt.% of V through the TRIP effect. However, the further increase of V content led to decreased RA content and stability, weakening the TRIP effect and resulting in a weaker strength ductility balance.

Abstract Background Transfemoral prosthesis users’ high fall rate is related to increased injury risk, medical costs, and fear of falling. Better understanding how stumble conditions (e.g., participant age, prosthesis type, side tripped, and swing phase of perturbation) affect transfemoral prosthesis users could provide insight into response deficiencies and inform fall prevention interventions. Methods Six unilateral transfemoral prosthesis users experienced obstacle perturbations to their sound limb in early, mid, and late swing phase. Fall outcome, recovery strategy, and kinematics of each response were recorded to characterize (1) recoveries versus falls for transfemoral prosthesis users and (2) prosthesis user recoveries versus healthy adult recoveries. Results Out of 26 stumbles, 15 resulted in falls with five of six transfemoral prosthesis users falling at least once. By contrast, in a previously published study of seven healthy adults comprising 214 stumbles using the same experimental apparatus, no participants fell. The two oldest prosthesis users fell after every stumble, stumbles in mid swing resulted in the most falls, and prosthesis type was not related to strategy/fall outcomes. Prosthesis users who recovered used the elevating strategy in early swing, lowering strategy in late swing, and elevating or lowering/delayed lowering with hopping in mid swing, but exhibited increased contralateral (prosthetic-side) thigh abduction and trunk flexion relative to healthy controls. Falls occurred if the tripped (sound) limb did not reach ample thigh/knee flexion to sufficiently clear the obstacle in the elevating step, or if the prosthetic limb did not facilitate a successful step response after the initial sound-side elevating or lowering step. Such responses generally led to smaller step lengths, less anterior foot positioning, and more forward trunk flexion/flexion velocity in the resulting foot-strikes. Conclusions Introducing training (e.g., muscle strength or task-specific motor skill) and/or modifying assistive devices (e.g., lower-limb prostheses or exoskeletons) may improve responses for transfemoral prosthesis users. Specifically, training or exoskeleton assistance could help facilitate sufficient thigh/knee flexion for elevating; training or prosthesis assistance could provide support-limb counteracting torques to aid in elevating; and training or prosthesis assistance could help initiate and safely complete prosthetic swing.

Copyright of Journal of Modern Turkish History Studies / Çagdas Türkiye Tarihi Arastirmalari Dergisi is the property of Journal of Modern Turkish History Studies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

tRip is a tRNA import protein specific to Plasmodium, the causative agent of malaria. In addition to its membrane localization and tRNA trafficking properties, tRip has the capacity to associate with three aminoacyl‐tRNA synthetases (aaRS), the glutamyl‐ (ERS), glutaminyl‐ (QRS), and methionyl‐ (MRS) tRNA synthetases. In eukaryotes, such multi‐aaRSs complexes (MSC) regulate the moonlighting activities of aaRSs. In Plasmodium, tRip and the three aaRSs all contain an N‐terminal GST‐like domain involved in the assembly of two independent complexes: the Q‐complex (tRip:ERS:QRS) and the M‐complex (tRip:ERS:MRS) with a 2:2:2 stoichiometry and in which the association of the GST‐like domains of tRip and ERS (tRip‐N:ERS‐N) is central. In this study, the crystal structure of the N‐terminal GST‐like domain of ERS was solved and made possible further investigation of the solution architecture of the Q‐ and M‐complexes by small‐angle x‐ray scattering (SAXS). This strategy relied on the engineering of a tRip‐N‐ERS‐N chimeric protein to study the structural scaffold of both Plasmodium MSCs and confirm the unique homodimerization pattern of tRip in solution. The biological impact of these structural arrangements is discussed. PDB Code(s): 8BCQ and 8BHD [ABSTRACT FROM AUTHOR]