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124 results on '"TREDICI, Salvatore Maurizio"'

2. Phenotyping of Fecal Microbiota of Winnie, a Rodent Model of Spontaneous Chronic Colitis, Reveals Specific Metabolic, Genotoxic, and Pro-inflammatory Properties

Author

Talà, Adelfia, Guerra, Flora, Resta, Silvia Caterina, Calcagnile, Matteo, Barca, Amilcare, Tredici, Salvatore Maurizio, De Donno, Maria Dolores, Vacca, Mirco, Liso, Marina, Chieppa, Marcello, De Angelis, Maria, Verri, Tiziano, Bozzetti, Maria Giuseppina, Bucci, Cecilia, and Alifano, Pietro

Published
2022
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7. Correlation between Photocatalysis and Antibacterial Responses of Cu‐Doped TiO2 Films: Influence of Doping Tuning into the Oxide Matrix

Author

Velardi, Luciano, primary, Scrimieri, Luigi, additional, Serra, Antonio, additional, Manno, Daniela, additional, Quarta, Gianluca, additional, Calcagnile, Lucio, additional, Calcagnile, Matteo, additional, Tredici, Salvatore Maurizio, additional, Alifano, Pietro, additional, Francioso, Luca, additional, and Signore, Maria Assunta, additional

Published
2023
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9. Phenotyping of fecal microbiota of Winnie, a rodent model of spontaneous chronic colitis, reveals specific metabolic, genotoxic, and pro-inflammatory properties

Author

Talà, Adelfia, primary, Guerra, Flora, additional, Resta, Silvia Caterina, additional, Calcagnile, Matteo, additional, Barca, Amilcare, additional, Tredici, Salvatore Maurizio, additional, De Donno, Maria Dolores, additional, Vacca, Mirco, additional, Liso, Marina, additional, Chieppa, Marcello, additional, De Angelis, Maria, additional, Verri, Tiziano, additional, Bozzetti, Maria Giuseppina, additional, Bucci, Cecilia, additional, and Alifano, Pietro, additional

Published
2022
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10. Rid7C, a Member of the YjgF/YER057c/UK114 (Rid) Protein Family, Is a Novel Endoribonuclease That Regulates the Expression of a Specialist RNA Polymerase Involved in Differentiation in Nonomuraea gerenzanensis

Author

Damiano, Fabrizio, primary, Calcagnile, Matteo, additional, Pasanisi, Daniela, additional, Talà, Adelfia, additional, Tredici, Salvatore Maurizio, additional, Giannotti, Laura, additional, Siculella, Luisa, additional, and Alifano, Pietro, additional

Published
2022
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11. Correlation between Photocatalysis and Antibacterial Responses of Cu‐Doped TiO2 Films: Influence of Doping Tuning into the Oxide Matrix.

Author

Velardi, Luciano, Scrimieri, Luigi, Serra, Antonio, Manno, Daniela, Quarta, Gianluca, Calcagnile, Lucio, Calcagnile, Matteo, Tredici, Salvatore Maurizio, Alifano, Pietro, Francioso, Luca, and Signore, Maria Assunta

Subjects
OXIDE coating, PHOTOCATALYSIS, RUTHERFORD backscattering spectrometry, PHOTOCATALYSTS, ATOMIC force microscopy, ELECTRON-hole recombination, SOL-gel processes
Abstract

Herein, a study on the photocatalysis and antibacterial performances of Cu‐doped titania films as a function of the dopant concentration is presented. The TiO2 films are produced by sol–gel spin‐coating technique, and the doping is performed by adding different quantities of copper in the form of salt (CuSO4) during the preparation of sol. The obtained films are characterized by Raman and X‐ray diffraction spectroscopies, atomic force microscopy, and Rutherford backscattering spectrometry, to investigate on the chemical–physical properties, the quality, the composition, and the thickness. The photocatalytic activity of the films is studied by measuring the degradation of methylene blue (MB) under UV light, while Staphylococcus aureus is chosen for microbiological testing. The results show a correlation between photocatalysis and antibactericity with a rise of these two activities with increasing dopant concentrations in the films up to a given point, beyond which structural defects are introduced, promoting electron–hole recombination. [ABSTRACT FROM AUTHOR]

Published
2023
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12. Chemotrophic profiling of prokaryotic communities thriving on organic and mineral nutrients in a submerged coastal cave

Author

Talà, Adelfia, primary, Buccolieri, Alessandro, additional, Calcagnile, Matteo, additional, Ciccarese, Gaetano, additional, Onorato, Michele, additional, Onorato, Raffaele, additional, Serra, Antonio, additional, Spedicato, Francesco, additional, Tredici, Salvatore Maurizio, additional, Alifano, Pietro, additional, and Belmonte, Genuario, additional

Published
2021
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17. TiO2 films by sol‐gel spin‐coating deposition with microbial antiadhesion properties.

Author

Scrimieri, Luigi, Serra, Antonio, Manno, Daniela, Alifano, Pietro, Tredici, Salvatore Maurizio, Calcagnile, Matteo, and Calcagnile, Lucio

Subjects
SOL-gel processes, SOL-gel materials, DRUG resistance in bacteria, BACTERIAL adhesion, MICROBIAL contamination, BACTERIAL cell surfaces, SURFACE contamination
Abstract

Intensive use of antibiotics induced adaptations in bacteria, which developed antibiotic resistance. This is becoming a serious health problem, particularly in the hospital, food industry, or public transport. It is also important to produce surfaces that not only are bactericidal but also prevent adhesion and the consequent biofilm formation, which can make the bacteria resistant to conventional disinfection methods. In this work, a simple and inexpensive method to obtain surfaces TiO2 film coated has been realized to prevent attachment and bacterial proliferation on surfaces. The synthesis and deposition procedure has been finalized to the realization of a uniform coating, whose physical, morphological, and structural features are suitable to inhibit the proliferation of the bacteria and in particular the adhesion of the biofilm. The suitability of the obtained coating has been attested by RBS, X‐ray diffraction (XRD), SEM, UV‐vis, and Raman techniques. The obtained coatings were homogeneous anatase titania films with an excellent adherence to the substrate and a transmittivity higher than 80% in the visible region. The results show that the TiO2 films considerably reduce microbial contamination on the surface (~98% reduction) feature that makes this coating suitable for antibacterial applications. [ABSTRACT FROM AUTHOR]

Published
2019
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20. Antibacterial properties of composite UHMWPE/{TiO}_{2-{x}} surfaces

Author

DELLE SIDE, DOMENICO, NASSISI, Vincenzo, GIUFFREDA, ERNESTO, VELARDI, LUCIANO, ALIFANO, Pietro, TALA', ADELFIA, TREDICI, Salvatore Maurizio, DELLE SIDE, Domenico, Nassisi, Vincenzo, Giuffreda, Ernesto, Velardi, Luciano, Alifano, Pietro, Tala', Adelfia, and Tredici, Salvatore Maurizio

Abstract

Due to the diffusion of severe pathogens, everyday life is exposed to the risks of contracting severe diseases. For this reason, efficient antimicrobial surfaces are of paramount importance. In this work we present the first evidences of a new technique to obtain an antibacterial ultra high molecular weight polyethylene based on a non- stoichiometric, visible light responsive, titanium oxide coating. The coating was obtained through a process in which titanium ions, resulting from laser ablation of a corresponding target, were accelerated and implanted on the samples. The samples were tested against a Staphylo- coccus aureus strain, in order to assay their antimicrobial efficacy. Results show that this treatment strongly dis- courages bacterial colonization of the treated surfaces.

Published
2014

22. Studio innovativo sugli effetti della radiofrequenzautilizzando vibrioni bioluminescenti

Author

NASSISI, Vincenzo, DELLE SIDE, DOMENICO, PALADINI, FABIO, VELARDI, LUCIANO, ALIFANO, Pietro, TREDICI, Salvatore Maurizio, BUCCOLIERI, Giovanni, R. Longo, S. Rizzato, M. V. Siciliano, TALA', ADELFIA, G. Palamà, Nassisi Paladini, Nassisi, Vincenzo, DELLE SIDE, Domenico, R., Longo, Paladini, Fabio, S., Rizzato, M. V., Siciliano, Velardi, Luciano, Alifano, Pietro, Tala', Adelfia, Tredici, Salvatore Maurizio, Buccolieri, Giovanni, and G., Palamà

Subjects
bioluminescenza, RF, Vibrio
Published
2012

23. Controllo di Biodeteriogeni via Laser Cleaning colonizzanti Volumi di Interesse Archeologico

Author

M. Abbracciavento, ALIFANO, Pietro, DELLE SIDE, DOMENICO, NASSISI, Vincenzo, PALADINI, FABIO, TREDICI, Salvatore Maurizio, VELARDI, LUCIANO, Nassisi Paladini, M., Abbracciavento, Alifano, Pietro, DELLE SIDE, Domenico, Nassisi, Vincenzo, Paladini, Fabio, Tredici, Salvatore Maurizio, and Velardi, Luciano

Subjects
Archeologia, Laser Cleaning, Biodeteriogeni
Published
2012

24. Caratterizzazione di Batteri Vibrio harveyi irradiati con luce UV e raggi X

Author

ALIFANO, Pietro, BUCCOLIERI, Giovanni, NASSISI, Vincenzo, PALADINI, FABIO, TREDICI, Salvatore Maurizio, G. Ciccarese, S. Rizzato, M. V. Siciliano, TALA', ADELFIA, Nassisi Paladini, Alifano, Pietro, G., Ciccarese, Buccolieri, Giovanni, Nassisi, Vincenzo, Paladini, Fabio, S., Rizzato, M. V., Siciliano, Tala', Adelfia, and Tredici, Salvatore Maurizio

Subjects
Vibrio batteri
Published
2012

25. Tecnica LIS utilizzata per rendere antibatterico l'UHMWPE

Author

SICILIANO, MARIA VITTORIA, TREDICI, Salvatore Maurizio, ALIFANO, Pietro, NASSISI, Vincenzo, TALA', ADELFIA, Società Italiana di Fisica, Siciliano, MARIA VITTORIA, Tala', Adelfia, Tredici, Salvatore Maurizio, Alifano, Pietro, and Nassisi, Vincenzo

Abstract

In questo lavoro si presentano i risultati ottenuti impiantando ioni di Cu e Ag su polietilene Ultra High Molecular Weight Polyethylene. L'impiantazione è stata eseguita utilizzando la tecnica LIS (Laser Ion Source), con laser ad eccimeri KrF e utilizzando una doppia accelerazione ottendo 60 kV di accelerazione. La fluenza laser è stata di 2.2 J/cm2 corrispondente ad un flusso ionico di 1012 ions/cm2 . Test di antibattericità sono stati condotti utilizzando il ceppo Staphylococcus aureus. Esami preliminari dimostrano una diminuzione della proliferazione di batteri sui campioni trattati.

Published
2011

26. Behavior of Vibrio harveyi bacteria under RF irradiation

Author

ALIFANO, Pietro, BUCCOLIERI, Giovanni, NASSISI, Vincenzo, PALADINI, FABIO, SICILIANO, MARIA VITTORIA, TREDICI, Salvatore Maurizio, Ciccarese G., TALA', ADELFIA, Longo R., Società Italiana di FISCIA, Alifano, Pietro, Buccolieri, Giovanni, Ciccarese, G., Nassisi, Vincenzo, Paladini, Fabio, Siciliano, MARIA VITTORIA, Tala', Adelfia, Tredici, Salvatore Maurizio, and Longo, R.

Abstract

Studies on Vibrio bacteria sampled from a coastal cave having an high radon content are presented. Most of the strains exhibited a low rate of survival after the UV exposure. All strains after UV and visible irradiation showed an high capability of photoreactivation. This capability was unexpected. We hypothesize that the photoreactivation might have been evolved to repair DNA lesions induced also by different radiations and that the luminescent bacteria might use their own light emission to carry out the photoreactivation. We assessed that the photoreactivation increased for some strains of about 42000 times.

Published
2011

27. Effect of UHTM frequencies on Vibrio harveyi bacteria

Author

ALIFANO, Pietro, NASSISI, Vincenzo, TREDICI, Salvatore Maurizio, VELARDI, LUCIANO, Longo R., Rizzato S., Siciliano M. V., TALA', ADELFIA, Società Italina di Fisica, Alifano, Pietro, Longo, R., Nassisi, Vincenzo, Rizzato, S., Siciliano, M. V., Tala', Adelfia, Tredici, Salvatore Maurizio, and Velardi, Luciano

Abstract

Bio-luminescent bacteria exposed to radiation of UHTM frequencies is not known and they offer new prospective to study modern biological sensors and DNA. We performed experiments undergoing an electric field of 53 and 140 V/m. Bioluminescence decreased of 77% and 68% undergone lower and higher electric field, respectively. We estimated a corresponding enhancement of the bioluminescent activity of bacteria growing at RF environment of 23 and 85 times undergone lower and higher electric field. This behavior of intense activity of bacteria on RF exposure can be ascribed to the anti-mutagenic effect of the RF.

Published
2011

28. Unexpected photoreactivation of Vibrio harveyi bacteria living in ionization environment

Author

ALIFANO, Pietro, NASSISI, Vincenzo, SICILIANO, MARIA VITTORIA, TALA', ADELFIA, TREDICI, Salvatore Maurizio, Alifano, Pietro, Nassisi, Vincenzo, Siciliano, MARIA VITTORIA, Tala', Adelfia, and Tredici, Salvatore Maurizio

Subjects
104703 (2011)
Abstract

Bacteria undergoing environmental effects is extremely interesting for structural, mechanistic, and evolutionary implications. Luminescent bacteria that have evolved in a specific ambient have developed particular responses and their behavior can give us new suggestions on the task and production of luciferina proteins. To analyze the UV interaction under controlled laboratory conditions, we used photoluminescent bacterial strains belonging to a new species evolutionarily close to Vibrio harveyi sampled from a coastal cave with a high radon content that generates ionizing radiation. The survival of the bacterial strains was analyzed, in the light and in the dark, following a variety of genotoxic treatments including UV radiation exposure. The strains were irradiated by a germicide lamp. The results demonstrated that most of the strains exhibited a low rate of survival after the UV exposure. After irradiation by visible light following the UV exposure, all strains showed a high capability of photoreactivation when grown. This capability was quite unexpected because these bacteria were sampled from a dark ambient without UV radiation. This leads us to hypothesize that the photoreactivation in these bacteria might have been evolved to repair DNA lesions also induced by different radiation sources other than UV (e.g., x-ray) and that the luminescent bacteria might use their own light emission to carry out the photoreactivation. The high capability of photoreactivation of these bacteria was also justified by the results of deconvolution. The deconvolution was applied to the emission spectra and it was able to show evidence of different light peaks. The presence of the visible peak could control the photolysis enzyme.

Published
2011

29. Behaviour of vibrio harveyi bacteria under RF irradation

Author

ALIFANO, Pietro, NASSISI, Vincenzo, PALADINI, FABIO, SICILIANO, MARIA VITTORIA, TALA', ADELFIA, TREDICI, Salvatore Maurizio, DE DONNO, Francesco, A. Rainò, L. Velardi, S. Rizzato, R. Longo, Società Italiana di Fisica, Alifano, Pietro, Nassisi, Vincenzo, Paladini, Fabio, A., Rainò, Siciliano, MARIA VITTORIA, Tala', Adelfia, Tredici, Salvatore Maurizio, L., Velardi, DE DONNO, Francesco, S., Rizzato, and R., Longo

Published
2010

31. DNA studies at 900 MHz EM fields by a TEM plane transmission line

Author

TALA', ADELFIA, LORUSSO, ANTONELLA, TREDICI, Salvatore Maurizio, NASSISI, Vincenzo, ALIFANO, Pietro, L. Velardi, A. Rainò, V. Nassisi, F. Paladini, M.V. Siciliano, Tala', Adelfia, Lorusso, Antonella, Tredici, Salvatore Maurizio, L., Velardi, Nassisi, Vincenzo, Alifano, Pietro, and A., Rainò

Published
2010

32. Vibrio harveyi bacteria irradiated by an X-ray flux

Author

SICILIANO, MARIA VITTORIA, ALIFANO, Pietro, BUCCOLIERI, Giovanni, NASSISI, Vincenzo, TALA', ADELFIA, TREDICI, Salvatore Maurizio, L. Velardi, Società Italiana di Fisica, Siciliano, MARIA VITTORIA, Alifano, Pietro, Buccolieri, Giovanni, Nassisi, Vincenzo, Tala', Adelfia, Tredici, Salvatore Maurizio, and L., Velardi

Published
2010

33. Microbes at work in perfumery: industrial use of bacteria to produce essences

Author

TALA', ADELFIA, CARATA, ELISABETTA, TREDICI, Salvatore Maurizio, ALIFANO, Pietro, Del Giudice L., Massardo D. R., Pontieri P., Bertea C., Mombello D., Mucciarelli M., De Stefano M., Vigliotta G., Maffei M. E., Tala', Adelfia, Carata, Elisabetta, Del Giudice, L., Massardo, D. R., Pontieri, P., Bertea, C., Mombello, D., Tredici, Salvatore Maurizio, Mucciarelli, M., De Stefano, M., Vigliotta, G., Maffei, M. E., and Alifano, Pietro

Subjects
Vetiver, root bacteria, biotransformation, terpene, essential oil
Published
2009

37. The microbial community of Vetiver root and its involvement into essential oil biogenesis

Author

DEL GIUDICE L, MASSARDO DR, PONTIERI P, BERTEA CM, MOMBELLO D, CARATA, ELISABETTA, TREDICI, Salvatore Maurizio, TALA', ADELFIA, MUCCIARELLI M, GROUDEVA VI, DE STEFANO M, VIGLIOTTA, Giovanni, MAFFEI ME, ALIFANO, Pietro, DEL GIUDICE, L, Massardo, D. R., Pontieri, P, Bertea, C. M., Mombello, D, Carata, E, Maurizio, S, Tredici, A. T., Mucciarelli, M, Groudeva, V. I., DE STEFANO, Mario, Vigliotta, G, Maffei, M. E., P., Alifano, Massardo, Dr, Bertea, Cm, Carata, Elisabetta, Tredici, Salvatore Maurizio, Tala', Adelfia, Groudeva, Vi, DE STEFANO, M, Vigliotta, Giovanni, Maffei, Me, and Alifano, Pietro

Subjects
Vetiveria, ZIZANIOIDES, Vetiveria zizanioides (L.) Nash, Plant-bacteria interaction, DIVERSITY, Vetiver essential oil biogenesi, RHIZOSPHERE, Sesquiterpene metabolism, SOIL, SP NOV, root-associated bacteria, GROWTH, PLANTS, BIOSYNTHESIS, Electron Microscopy, Bacteria (microorganisms), BACTERIAL COMMUNITIES, NEMATOCIDAL ACTIVITY
Abstract

Vetiver is the only grass cultivated worldwide for the root essential oil, which is a mixture of sesquiterpene alcohols and hydrocarbons, used extensively in perfumery and cosmetics. Light and transmission electron microscopy demonstrated the presence of bacteria in the cortical parenchymatous essential oil-producing cells and in the lysigen lacunae in close association with the essential oil. This finding and the evidence that axenic Vetiver produces in vitro only trace amounts of oil with a strikingly different composition compared with the oils from in vivo Vetiver plants stimulated the hypothesis of an involvement of these bacteria in the oil metabolism. We used culture-based and culture-independent approaches to analyse the microbial community of the Vetiver root. Results demonstrate a broad phylogenetic spectrum of bacteria, including α-, β- and γ-Proteobacteria, high-G+C-content Gram-positive bacteria, and microbes belonging to the Fibrobacteres/Acidobacteria group. We isolated root-associated bacteria and showed that most of them are able to grow by using oil sesquiterpenes as a carbon source and to metabolize them releasing into the medium a large number of compounds typically found in commercial Vetiver oils. Several bacteria were also able to induce gene expression of a Vetiver sesquiterpene synthase. These results support the intriguing hypothesis that bacteria may have a role in essential oil biosynthesis opening the possibility to use them to manoeuvre the Vetiver oil molecular structure. © 2008 The Authors.

Published
2008

41. Nitrite metabolism in tobacco yeast strain Debaryomyces hansenii TOB-Y7

Author

PARENTE D, FIORENTINO F, SORRENTINO F. A, STANZIONE F, MASSARDO D. R, PONTIERI P, DEL GIUDICE L, CARATA, ELISABETTA, TREDICI, Salvatore Maurizio, VIGLIOTTA, Giovanni, ALIFANO, Pietro, Parente, D, Fiorentino, F, SORRENTINO F., A, Stanzione, F, MASSARDO D., R, Pontieri, P, DEL GIUDICE, L, Carata, Elisabetta, Tredici, Salvatore Maurizio, Vigliotta, Giovanni, and Alifano, Pietro

Subjects
Tobacco fermentation, Debaryomyces hansenii, Tobacco specific nitrosamines
Published
2007

43. Duplication of the RNA polymerase beta-chain encoding gene in Actinomycetes

Author

VIGLIOTTA, Giovanni, TREDICI, Salvatore Maurizio, DAMIANO, FABRIZIO, DI SUMMA, ROBERTA, GNONI, Gabriele Vincenzo, ALIFANO, Pietro, MONTINARO M. R, PULIMENO R, MONTANARO V, Vigliotta, Giovanni, Tredici, Salvatore Maurizio, Damiano, Fabrizio, MONTINARO M., R, Pulimeno, R, DI SUMMA, Roberta, Montanaro, V, Gnoni, Gabriele Vincenzo, and Alifano, Pietro

Subjects
RNA polymerase, Nonomuraea sp. ATCC 39727, Glycopeptide antibiotic, rpoB, Rifampicin
Published
2005

44. A new technique by a suitable plane transmission line at 900 MHz RF fields for E. coli DNA studies

Author

BELLONI, fabio, NASSISI, Vincenzo, ALIFANO, Pietro, MONACO, Caterina, TALA', ADELFIA, TREDICI, Salvatore Maurizio, RAINO' A., Belloni, Fabio, Nassisi, Vincenzo, Alifano, Pietro, Monaco, Caterina, Tala', Adelfia, Tredici, Salvatore Maurizio, and Raino', A.

Subjects
Radiofrequency, DNA mismatch repair, Escherichia coli, Mutagenesi
Abstract

Utilizing a suitable irradiating transmission line chamber, the effects of 900 MHz radio frequency fields on DNA mutability and repair in Escherichia coli strains were investigated. The transmission line was very versatile and able to easily apply different field values. In this experiment the maximum electric and magnetic fields were 66 V/m and 260 nT, respectively, in the absence of cell plates. Slight decrease in spontaneous mutability to erythromycin resistance was demonstrated in mismatch-repair proficient bacteria exposed to the radio frequency electromagnetic field sRF-EMFd during their growth on solid medium. The anti-mutagenic effect of the RF-EMF was much more impressive at the level of a hypermutagenic cytosine repeat, whose stability is strongly dependent on the activity of the mismatch repair system. In contrast, in mismatch repair-defective background RF-EMF neither affected the general DNA mutability nor the stability of the cytosine repeat, suggesting that the anti-mutagenic effect of the 900 MHz RF-EMF might be due to an improved efficiency of the mismatch repair system.

Published
2005

45. Misure di campi elettromagnetici responsabili dei processi di mutazione indotti su microrganismi

Author

BELLONI, fabio, DORIA, Domenico, LORUSSO, ANTONELLA, NASSISI, Vincenzo, ALIFANO, Pietro, MONACO, Caterina, TALA', ADELFIA, TREDICI, Salvatore Maurizio, RAINO' A., Belloni, Fabio, Doria, Domenico, Lorusso, Antonella, Nassisi, Vincenzo, Alifano, Pietro, Monaco, Caterina, Tala', Adelfia, Tredici, Salvatore Maurizio, and Raino', A.

Subjects
Bacteria, Bioindicator, Mutagenesis, Electromagnetic field
Published
2005

46. Analisi della regolazione del gene gdhA (L-glutammato deidrogenasi) in isolati clinici di Neisseria meningitidis

Author

PAGLIARULO C, SALVATORE P, COLICCHIO R, BRUNI C. B, DE VITIS, Lucia, MONACO, Caterina, TREDICI, Salvatore Maurizio, TALA', ADELFIA, ALIFANO, Pietro, Pagliarulo, C, Salvatore, P, Colicchio, R, DE VITIS, Lucia, Monaco, Caterina, Tredici, Salvatore Maurizio, Tala', Adelfia, BRUNI C., B, and Alifano, Pietro

Subjects
Neisseria meningitidi, Transcription regulation, NADP specific L glutamate dehydrogenase
Published
2004

47. Regulation and differential expression of gdhA encoding NADP-specific glutamate dehydrogenase in Neisseria meningitidis clinical isolates

Author

PAGLIARULO C, SALVATORE P, COLICCHIO R, BARDARO M, LAVITOLA A, DE VITIS, Lucia, MONACO, Caterina, TREDICI, Salvatore Maurizio, TALA', ADELFIA, ALIFANO, Pietro, Pagliarulo, C, Salvatore, P, DE VITIS, Lucia, Colicchio, R, Monaco, Caterina, Tredici, Salvatore Maurizio, Tala', Adelfia, Bardaro, M, Lavitola, A, and Alifano, Pietro

Subjects
Meningococcal metabolism, Transcriptional regulation, Carbon source regulation, Meningococcal pathogenesi, NADP specific L glutamate dehydrogenase
Abstract

Meningococcal gdhA, encoding the NADP-specific L-glutamate dehydrogenase (NADP-GDH), is essential for systemic infection in an infant rat model. In this paper, a limited transcriptional analysis detected differences in gdhA expression among clinical isolates. In strains expressing high levels of gdhA mRNA, two promoters, gdhA P1 and gdhA P2, initiated transcription of gdhA. In contrast, in strains expressing low mRNA levels, gdhA P2 was not active because of weak expression of gdhR, an associated regulatory gene. Gene knock-out and complementation of a gdhR-defective mutant confirmed that GdhR is a positive regulator for gdhA P2. Trans-activation of gdhA P2 was maximal in complex medium during late logarithmic growth phase and in chemical defined medium (MCDA) when glucose (MCDA-glucose) instead of lactate (MCDA-lactate) was used as a carbon source in the presence of glutamate. gdhR knock-out mutants lost both growth phase and carbon source regulation, and exhibited a growth defect more severe in MCDA-glucose than in MCDA-lactate. DNA-protein interaction studies demonstrated that 2-oxoglutarate, a product of the catabolic reaction of the NADP-GDH and an intermediate of the tricarboxylic acid (TCA) cycle, inhibits binding of GdhR to gdhA P2.

Published
2004

48. Ruolo delle translated repeats (TR) nel gene mfd codificante il fattore di accoppiamento trascrizione/riparazione in isolati clinici di Neisseria meningitidis

Author

COLICCHIO R, SALVATORE P, PAGLIARULO C, LAMBERTI F, BRUNI C. B, VIGLIOTTA, Giovanni, TREDICI, Salvatore Maurizio, ALIFANO, Pietro, Colicchio, R, Salvatore, P, Pagliarulo, C, Lamberti, F, Vigliotta, Giovanni, Tredici, Salvatore Maurizio, BRUNI C., B, and Alifano, Pietro

Subjects
Transcriptional coupled DNA repair, Hypermutation, Neisseria meningitidi, mfd
Published
2004

49. Studio di spore di Attinomiceti irraggiate tramite laser XeCl

Author

BELLONI, fabio, DORIA, Domenico, LORUSSO, ANTONELLA, NASSISI, Vincenzo, ALIFANO, Pietro, MONACO, Caterina, TREDICI, Salvatore Maurizio, NUZZACI A, Belloni, Fabio, Doria, Domenico, Lorusso, Antonella, Nuzzaci, A, Nassisi, Vincenzo, Alifano, Pietro, Monaco, Caterina, and Tredici, Salvatore Maurizio

Subjects
Actinomycete, XeCl excimer laser, Mutagenesi
Published
2004

50. Ruolo di GdhR nella regolazione dell’ espressione di gdhA codificante la L-glutammato deidrogenasi NADP-specifica, in isolati clinici di Neisseria meningitidis

Author

PAGLIARULO C, COLICCHIO R, SALVATORE P, MONTUORI I, BRUNI C. B, DE VITIS, Lucia, MONACO, Caterina, TREDICI, Salvatore Maurizio, TALA', ADELFIA, ALIFANO, Pietro, Pagliarulo, C, Colicchio, R, Salvatore, P, Montuori, I, DE VITIS, Lucia, Monaco, Caterina, Tredici, Salvatore Maurizio, Tala', Adelfia, BRUNI C., B, and Alifano, Pietro

Subjects
Neisseria meningitidi, Transcription regulation, NADP specific L glutamate dehydrogenase
Published
2004

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