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The Pro‐Pro ligated tripeptides Ile‐Pro‐Pro 4 (IPP) and Val‐Pro‐Pro 5 (VPP) isolated from the fermented milk exhibit marked anti‐hypertension activity. Additionally, the naturally occurring Pro‐Pro ligated tripeptide Gly‐Pro‐Pro 6 (GPP) exhibits antioxidant properties. These peptides have enormous potential to be used as dilatory supplements. In the present study, we achieved a scalable, convenient, and high‐yielding synthesis of IPP, VPP, and GPP along with two more hydrophobic Pro‐Pro ligated tripeptides (APP 7, LPP 8) by employing the TAG technique. We used acid labile (2,4‐bis(octadecyloxy)phenyl)methanol as the TAG. We demonstrated synthesis of one of IPP at molar scale. We think that our method has the potential to be escalated to an industrial scale without much difficulty and they become available for general use as dietary supplements. [ABSTRACT FROM AUTHOR]

Tiger nut is the tuber of the perennial herbaceous plant Cyperus esculentus L., whose unique underground tubers are not only the main reproductive organ but also an important oil storage site. Oleosin is the most abundant structural protein in the oil body, which is an important membrane structural protein, playing a role in the formation and stability of lipid droplets in oilseed crops. Most studies have focused on the oleosin in oilseeds, but rarely on the oil containing tuber. In this study, nine oleosin genes from the Cyperus esculentus transcriptome were identified and divided into two groups via phylogenetic analysis. The expression patterns of the nine oleosins were examined through quantitative real-time PCR (qRT-PCR) in various development stages of stem tissue (35 d, 50 d, 75 d, 90 d, and 120 d after sowing). The subcellular localization of CeOle4 indicated that this protein was localized exclusively to membrane, indicating that it functioned in the plasma membrane. The highly expressed gene CeOle4 within the CeOleosin gene family was further transformed into yeast cells and plant materials. The results demonstrate that CeOle4 can promote lipid synthesis, enhancing the stability of oil lipids at low temperature and changing seed phenotypic traits. This discovery addresses and enriches the research on the function of CeOleosin genes and lays the groundwork for future studies on novel and superior transgenic crops related to tiger nut. [ABSTRACT FROM AUTHOR]

Despite the high diversity of elasmobranch fishes in the Balearic Islands, knowledge of their biology and population dynamics is still scarce. A recent mark-and-recapture experiment off the Balearic Islands tagged 3738 individuals of 23 shark and batoid species during MEDITS and CANAL bottom trawl scientific surveys from June 2021 to August 2023. Retrieval was reported for the sharks Scyliorhinus canicula and Mustelus mustelus, revealing relatively small home ranges for these species (0.2–38.5 km and 7.8–15.3 km for S. canicula and M. mustelus, respectively). Recapture efficiency was higher from scientific surveys than from commercial catches, highlighting potential challenges in collaboration with fishermen and recapture reports. Density estimates obtained from the MEDITS bottom trawl survey suggest a much larger population for S. canicula than estimates from the mark–recapture data, indicating MEDITS density estimates for this species may be overestimated due to its scavenger behavior perhaps favoring individuals searching for discards aggregated in the fishing grounds. This study emphasizes the importance of monitoring, collaborative efforts, and improved reporting mechanisms to enhance our understanding of elasmobranch populations and provide support for sustainable management of these vulnerable marine species. [ABSTRACT FROM AUTHOR]

Obesity remains one of the largest health problems in the world, arising from the excess storage of triglycerides (TAGs). However, the full complement of genes that are important for regulating TAG storage is not known. The Glut1 gene encodes a Drosophila glucose transporter that has been identified as a potential obesity gene through genetic screening. Yet, the tissue-specific metabolic functions of Glut1 are not fully understood. Here, we characterized the role of Glut1 in the fly brain by decreasing neuronal Glut1 levels with RNAi and measuring glycogen and TAGs. Glut1RNAi flies had decreased TAG and glycogen levels, suggesting a nonautonomous role of Glut1 in the fly brain to regulate nutrient storage. A group of hormones that regulate metabolism and are expressed in the fly brain are Drosophila insulin-like peptides (Ilps) 2, 3, and 5. Interestingly, we observed blunted Ilp3 and Ilp5 expression in neuronal Glut1RNAi flies, suggesting Glut1 functions in insulin-producing neurons (IPCs) to regulate whole-organism TAG and glycogen storage. Consistent with this hypothesis, we also saw fewer TAGs and glycogens and decreased expression of Ilp3 and Ilp5 in flies with IPC-specific Glut1RNAi. Together, these data suggest Glut1 functions as a nutrient sensor in IPCs, controlling TAG and glycogen storage and regulating systemic energy homeostasis. [ABSTRACT FROM AUTHOR]

Key message: CsDGAT1A and CsDGAT2D play a positive regulatory role in cucumber's response to low-temperature stress and positively regulate the synthesis of triacylglycerol (TAG). Triacylglycerol (TAG), a highly abundant and significant organic compound in plants, plays crucial roles in plant growth, development, and stress responses. The final acetylation step of TAG synthesis is catalyzed by diacylglycerol acyltransferases (DGATs). However, the involvement of DGATs in cucumber's low-temperature stress response remains unexplored. This study focused on two DGAT genes, CsDGAT1A and CsDGAT2D, investigating their function in enhancing cucumber’s low-temperature stress tolerance. Our results revealed that both proteins were the members of the diacylglycerol acyltransferase family and were predominantly localized in the endoplasmic reticulum. Functional analysis demonstrated that transient silencing of CsDGAT1A and CsDGAT2D significantly compromised cucumber’s low-temperature stress tolerance, whereas transient overexpression enhanced it. Furthermore, the TAG content quantification indicated that CsDGAT1A and CsDGAT2D promoted TAG accumulation. In conclusion, this study elucidates the lipid metabolism mechanism in cucumber’s low-temperature stress response and offers valuable insights for the cultivation of cold-tolerant cucumber plants. [ABSTRACT FROM AUTHOR]

Nutritional education is pivotal in the medical nutritional therapy of type 2 diabetes mellitus (T2DM). The extended parallel process model (EPPM) is a health education method for inducing desirable health behaviours. The present study aimed to investigate the effect of nutritional education based on the EPPM in T2DM patients on knowledge, attitude, practice, anthropometric indices, glycaemic factors, lipid profile and adherence to the diabetic diet. A randomised, double-blind, controlled, factorial field trial was designed for T2DM patients aged 30–59 years (n 88). Participants were randomly allocated into four groups to receive EPPM-based nutritional education through gain framed message (GFM), loss framed message (LFM), their combination (G\LFM) or usual diabetic education in the control group (CG). Participants were assessed before and after the study duration. After 3 months of intervention, eighty participants finished the study. The EPPM-based intervention increased participants' knowledge, behavioural intention, perceived sensitivity, severity, self-efficacy (P < 0·001 for all) and response efficacy (P = 0·029) in comparison with CG. GFM (P = 0·004) and G\FLM (P = 0·034) reduced carbohydrate intake and LFM (P = 0·034) and G\LFM (P = 0·047) decreased fat intake. Between-group analysis indicated interventions reduced weight (P = 0·046), BMI (P = 0·038), fasting blood sugar (P = 0·030), 2-hour postprandial blood glucose (P = 0·027) and TAG (P = 0·002) in comparison with the CG. Results were NS for protein intake, waist and hip circumference, waist:hip ratio, HbA1c, total cholesterol, LDL and HDL. Nutritional education based on EPPM could increase the knowledge and awareness of T2DM patients. Also, it could be beneficial for blood glucose amendment. Further investigations are recommended. [ABSTRACT FROM AUTHOR]

This article introduces the possibility of studying graffiti using a colorimeter developed with Arduino hardware technology according to the Do It Yourself (DIY) philosophy. Through the obtained Red Green Blue (RGB) data it is intended to study and compare the information extracted from each of the graffiti present on different walls. The same color can be found in different parts of a single graffiti, but also in other graffiti that could a priori be of different authorship. Nevertheless, graffiti may be related, and it may be possible to group graffiti artists and "gangs" that work together. The methodology followed for the construction of the colorimeter and its real application in a practical case are described in four case studies. The case studies describe how graffiti were identified and recognized and they provide a comparison of the collected color samples. The results show the added value of the colorimeter in the graffiti recognition process, demonstrating its usefulness on a functional level. Finally, the contributions of this research are outlined, and an analysis is carried out of the changes to be made to the proposed method in the future, for improved graffiti color identification. [ABSTRACT FROM AUTHOR]

This article explores the development of a new gene editing tool, ePE6d, which has shown promise in efficiently inserting tags into the rice genome. The researchers compared ePE6d with a previous tool, ePE2, and found that ePE6d performed better in terms of tag insertions. They also tested ePE6d's ability to insert longer tags and found success in certain cases. This study suggests that ePE6d could be a valuable tool for precise gene editing and tagging in rice plants. [Extracted from the article]

The present paper introduces the analysis and design of a near-field RFID tag for IoT in the sub-six GHz 5G frequency band, the proposed radio frequency identification technique is based on the near-field interaction between the RFID tag and a wideband antenna reader. This near-field interaction adjusts the resonances of the wideband antenna according to the used RFID tag. In addition, the far field RCS of the RFID tag is also investigated to study the relationship between the near field and the far field responses of the proposed RFID tag. The proposed RFID tag is characterized by adjustable six resonances based on concentric square rings printed on a dielectric slab. For manufacturing and experimental verification, the dielectric slab is assumed to be FR-4. However, the proposed structure can be generalized to other thin and flexible substrates like paper, plastic, and textile.

The object of this study is modern Human-Machine Interfaces (HMI) and SCADA systems in the industry. The subject of research is techniques for optimizing the number of tags (variables) in the SCADA/HMI environment to enhance resource utilization efficiency. One of the challenges in creating SCADA/HMI-based solutions can be the number of tags (variables) in the runtime environment. A large number of tags can lead to a problem of limited available resources. The technique presented here allow for the optimization of the number of tags used in HumanMachine Interface systems built with SCADA software and operator panels in combination with Programmable Logic Controllers (PLCs). An evaluation of the efficiency of techniques for reducing the number of HMI tags was conducted on an experimental configuration consisting of objects such as discrete input/output, analog input/output, actuators such as valves with discrete/analog control, and drives with frequency converters. The optimization coefficient, defined as the ratio of the number of input/output tags used directly to the number of tags after applying the optimization principle, was used as the efficiency criterion. Depending on the techniques and their combinations, the criterion values reached orders of 4, 10, and in one case even more than 100. These values are explained by the application of multiplexing approaches and various packing techniques. The advantages and disadvantages of the reported techniques, as well as their application limitations, have been identified. Some techniques are suitable only for specific tasks. These techniques could be applied in practical implementation when designing modern high-efficiency Human-Machine Interfaces under conditions of limited resources. [ABSTRACT FROM AUTHOR]

Thlaspi arvense (Pennycress) is an emerging feedstock for biofuel production because of its high seed oil content enriched in erucic acid. A transcriptomic and a lipidomic study were performed to analyze the dynamics of gene expression, glycerolipid content and acyl-group distribution during seed maturation. Genes involved in fatty acid biosynthesis were expressed at the early stages of seed maturation. Genes encoding enzymes of the Kennedy pathway like diacylglycerol acyltransferase1 (TaDGATl), lysophosphatidic acid acyltransferase (TaLPAT) or glycerol 3-phosphate acyltransferase (TaGPAT) increased their expression with maturation, coinciding with the increase in triacylglycerol species containing 22:1. Positional analysis showed that the most abundant triacylglycerol species contained 18:2 at sn-2 position in all maturation stages, suggesting no specificity of the lysophosphatidic acid acyltransferase for very long chain fatty acids. Diacylglycerol acyltransferase2 (TaDGAT2) mRNA was more abundant at the initial maturation stages, coincident with the rapid incorporation of 22:1 to triacylglycerol, suggesting a coordination between Diacylglycerol acyltransferase enzymes for triacylglycerol biosynthesis. Genes encoding the phospholipid-diacylglycerol acyltransferase (TaPDAT1), lysophosphatidylcholine acyltransferase (TaLPCAT) or phosphatidylcholine diacylglycerolcholine phosphotransferase (TaPDCT), involved in acyl-editing or phosphatidyl-choline (PC)-derived diacylglycerol (DAG) biosynthesis showed also higher expression at the early maturation stages, coinciding with a higher proportion of triacylglycerol containing C18 fatty acids. These results suggested a higher contribution of these two pathways at the early stages of seed maturation. Lipidomic analysis of the content and acyl-group distribution of diacylglycerol and phosphatidyl-choline pools was compatible with the acyl content in triacylglycerol at the different maturation stages. Our data point to a model in which a strong temporal coordination between pathways and isoforms in each pathway, both at the expression and acyl-group incorporation, contribute to high erucic triacylglycerol accumulation in Pennycress. [ABSTRACT FROM AUTHOR]

Hydroxyl dicarboxylic acids (OHDCA) are ubiquitous in the atmosphere as an important constituent of secondary organic aerosol, yet the formation mechanisms remain unclear. At an urban background site on the coast of South China, we observed notable levels of OHDCA, with the highest concentration of malic acid (a typical OHDCA species) reaching 533 ng m−3. In the coastal air, the correlation between OHDCA and sulfate was better (R2 = 0.48) in the period when the relative humidity was higher and the sulfate size distribution was in a droplet mode, fitting the features of aqueous formation. In the short‐range continental air, a significant rise in OHDCA levels from morning through early afternoon (588 ng m−3) was observed under marked daytime increment of ozone that was corrected for titration loss (O3_corr, sum of ozone and nitrogen dioxide). In addition, good correlation between OHDCA and O3_corr was identified in this period, illuminating the role for gas‐phase photochemistry in regulating OHDCA formation. Therefore, the elevated OHDCA was likely attributed to aqueous photooxidation, and the dominant factors varied under different atmospheric conditions. The precursors of OHDCA could be derived from biogenic emissions, as indicated by the correlations of OHDCA with 2‐methylglyceric acid (bihourly data) and isoprene and monoterpenes (daily average data). However, anthropogenic aromatics might also be involved in OHDCA formation, especially in the short‐range continental air. The formation mechanisms probed through observational evidence will be an important reference for rectifying simulations of OHDCA and its impact on air quality and climate. Plain Language Summary: Organic aerosol, a vital component of fine particulate matter, is suspended in the atmosphere. It is of widespread concern due to health and climate effects. In a coastal area generally considered clean, we observed remarkably high levels of climate‐relevant hydroxyl dicarboxylic acids in organic aerosol. Different factors dominated the aqueous photochemical formation of hydroxyl dicarboxylic acids between air masses arriving at the measurement site. This provides a scientific basis for mitigating organic aerosol and its climate effects. Key Points: The concentrations of hydroxyl dicarboxylic acids (OHDCA) in Hong Kong's air were among the highest in the worldAqueous and photochemical processes dominated OHDCA formation in the coastal and short‐range continental air, respectivelyAromatic compounds were likely one of the precursors of OHDCA in continental outflows [ABSTRACT FROM AUTHOR]

Utilization of marine photovoltaic energy is primarily focused on surface harvesting with limited photovoltaic cell implementations in submarine environments. Potential applications include marine wildlife telemetry devices, autonomous underwater vehicles, or remote sensing assets. In these applications, understanding the power at depth is critical, but there has yet to be a long‐term study of cell performance in a realistic marine environment that spans time, geographic location, and depth. In this paper, we present photovoltaic assessments carried out by devices mounted to adult female northern elephant seals (Mirounga angustirostris) during their spring migrations in the Pacific Ocean with deployment times between 76 and 107 days. Encompassing a large geographic area between Santa Cruz, California, and the Aleutian Islands of Alaska during their migrations, elephant seal behavior allows for repeated depth profiles each day, making them an ideal host for subsurface power assessments. This paper presents the first longitudinal study of photovoltaic cell performance in the marine environment that spans location, time, and depth. This work discusses the calibration, data time alignment, and power calculations of these oceanic deployments. Deployment results, including power results and energy predictions from the data record, are presented up to 22 m in depth. We highlight how the recorded power data of these cells compares to previously published results and how depth impacts subsurface power and energy harvesting. [ABSTRACT FROM AUTHOR]

In wireless sensor networks, sensing tags are battery powered, which also limits the battery life. Therefore, it is particularly important to study self powered sensing tags. At present, passive wireless identification systems can achieve self powered communication through tags. However, wireless power supply sensing systems have problems such as low efficiency and short communication distance. To reduce the power consumption of sensing tags and improve the efficiency of sensing systems, a passive wireless low-power hydrogen concentration sensing system is designed. To further reduce the power consumption of sensing tags, a passive wireless magnetic field intensity sensing system is developed. In addition, to solve the low efficiency of multiple sensing labels in wireless power supply sensing systems, an efficient anti-collision algorithm based on dynamic binary query trees is designed. According to the research results, in the cold start indoor environment, the energy collection rate of the sensing tag designed in the research was 0.081mJ/s, and the energy collection efficiency was 25.7%. In single/dual sensor mode, the maximum values of sensing error were 2.1% and 5.2%, respectively. The maximum recognition time of the anti-collision algorithm based on dynamic multi base query tree was 2.78s, and the minimum value was 1.31s. The research results can provide methodological and technical support for the improvement of wireless power supply sensing systems, expanding the application range of wireless power supply sensing systems.

Abstract Background Amphora coffeaeformis, a unicellular diatom, can significantly accumulate lipids under nitrogen (N) limitation. However, the molecular mechanism underlying lipid accumulation in A. coffeaeformis remains unknown and its application development is lagging. Results This work analyzed the lipid composition of A. coffeaeformis under N deprivation and investigated its mechanism underlying lipid accumulation using RNA-seq. The results showed that the total lipid content of A. coffeaeformis increased from 28.22 to 44.05% after 5 days of N deprivation, while the neutral lipid triacylglycerol (TAG) content increased from 10.41 to 25.21%. The transcriptional profile showed that N deprivation induced wide-ranging reprogramming of regulation and that most physiological activities were repressed, while the upregulation of glycerol-3-phosphate acyltransferase directly determined TAG accumulation. Moreover, we explored the effect of A. coffeaeformis as a food additive on the lipid composition of crucian carp. The results showed that the contents of unsaturated fatty acids in the meat of fish supplemented with A. coffeaeformis were significantly increased, indicating its potential application in animal nutrition for improving meat quality indicators. Conclusion The findings shed light on the molecular mechanisms of neutral lipid accumulation and revealed the key genes involved in lipid metabolism in A. coffeaeformis. Moreover, we also confirmed that A. coffeaeformis can be used as feed additive for improving the lipid composition of crucian carp meat, which provided evidence for the biotechnology application of this high-oil microalgae.

Tiger nut is the tuber of the perennial herbaceous plant Cyperus esculentus L., whose unique underground tubers are not only the main reproductive organ but also an important oil storage site. Oleosin is the most abundant structural protein in the oil body, which is an important membrane structural protein, playing a role in the formation and stability of lipid droplets in oilseed crops. Most studies have focused on the oleosin in oilseeds, but rarely on the oil containing tuber. In this study, nine oleosin genes from the Cyperus esculentus transcriptome were identified and divided into two groups via phylogenetic analysis. The expression patterns of the nine oleosins were examined through quantitative real-time PCR (qRT-PCR) in various development stages of stem tissue (35 d, 50 d, 75 d, 90 d, and 120 d after sowing). The subcellular localization of CeOle4 indicated that this protein was localized exclusively to membrane, indicating that it functioned in the plasma membrane. The highly expressed gene CeOle4 within the CeOleosin gene family was further transformed into yeast cells and plant materials. The results demonstrate that CeOle4 can promote lipid synthesis, enhancing the stability of oil lipids at low temperature and changing seed phenotypic traits. This discovery addresses and enriches the research on the function of CeOleosin genes and lays the groundwork for future studies on novel and superior transgenic crops related to tiger nut.

This review presents evidence related to the postprandial responses after consumption of dairy products focusing on the effect of the dairy matrix and lipid response, which was also presented as part of a speech at the Nutrition Society Winter Conference, January 2023. The key findings are that the dairy product(s) that differentiate from others in the postprandial TAG response are products with a semi-solid structure. There were no differences in lipid responses between cheese and butter. The main factors viscosity, fat globule size and milk fat globule membrane do not seem to explain the effect of the dairy matrix in the acute postprandial response. In summary, it is very difficult to investigate the effects of the dairy matrix per see and with the few studies conducted to date, no clear cause and effect can be established. Future research should focus on the semi-solid dairy matrix, and studies investigating specifically the yoghurt matrix are warranted. [ABSTRACT FROM AUTHOR]

Copyright of Al-Academy is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The marking or tagging of turtles is a widely used to facilitate the monitoring of population demography, structure, and conservation of species. Even though they are used in many studies of turtle demography, the available methods are not very efficient, except for microchips (Passive Intergraded Transponder/PIT tags); however, the latter is too expensive to be used on a large scale. Here we present a viable, cost-effective tagging alternative that uses a durable plastic cattle tag and pin. Turtles in the genus Podocnemis were tagged in the Lower-Amazon region, west of the state of Para, Amazon, Brazil. This method is highly recommended for future work on monitoring and population structure of large species of freshwater turtles. In addition to feasibility, it can be used in the form of citizen science, as the information can be obtained by laypeople and taken to researchers and conservationists. [ABSTRACT FROM AUTHOR]

Vehicular Internet of Things (VIoT) refers to integrating Internet of Things (IoT) technology into the transportation sector, specifically vehicles. It aims to enhance transportation safety, efficiency, and sustainability by using connected devices and real-time data exchange between vehicles, road infrastructure, and transportation management systems. RFID can provide valuable insights into the functioning and performance of vehicles, enabling the development of intelligent transportation systems and contributing to the growth of the VIoT. To secure essential driving data tag location, data privacy, and road safety are the primary concerns in the VIoT system. An attacker can perform a variety of attacks on the tag and compromise its privacy through the wireless channel between it and the reader. To ensure secure communication for the various application of the VIoT system, An RFID-based protocol is proposed, which is referred to as UPSRVNet (Ultralightweight, Privacy preserved, and Secure RFID-based authentication protocol for VIoT Networks). The proposed protocol guarantees secure authentication in VIoT networks using ultralightweight, privacy-preserving RFID tags by substituting bit formation and right-shift rotation for high-computing operations like hash functions and encryption/decryption algorithms. It also reduces RFID tags' storage and communication overhead and aims to secure the driver's location, personal information, and resistance from known attacks. As per the experiment result, the proposed UPSRVNet protocol showed a significant reduction in communication overhead between tag and reader, that is 50%, and the storage cost on the tag is also reduced by 33.33% compared to existing protocols. Due to this, the certification and authentication processes may be performed quickly. The protocol ensured the security requirements by informal analysis using the Scyther tool. A comprehensive analysis of the proposed protocol reveals its substantial advantages over the existing protocol concerning computation complexity, communication efficacy, and storage expenditure. [ABSTRACT FROM AUTHOR]

Copyright of Bulletin of Ablai Khan KazUIRandWL: Series 'Philological sciences' is the property of Kazakh Ablai Khan University of International Relations & World Languages and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This paper presents a method that enables a small car to reach its destination in an indoor environment using ultrawideband technology. For this purpose, we used four anchors and one tag. The tag can move because it is fixed on the car. The anchors and the tag are DWM1001 modules. The configuration of the five DW1001 modules is performed through a smartphone application provided by the manufacturer. The car has been built by us. The controller of the car is a Raspberry Pi microcomputer which controls the driver of the car DC motors by generating PWM signals. In addition, Raspberry Pi communicates with the tag by the UART interface. Thus, our goal was to control the car to reach a certain destination (xd, yd) requested by the user via Wi-Fi. Because the car hosts the tag, it continuously updates its position according to the information received from the four anchors. We implemented two scenarios, considering the initial (x, y) position of the car. In the first scenario, which is simpler, the car moved to its destination either along the x-axis or along the y-axis. In the second scenario, the car moves along one of the axes and then turns 90 degrees to reach its destination. Experimental results that show the precision of the positioning system are presented. [ABSTRACT FROM AUTHOR]

Background: Amphora coffeaeformis, a unicellular diatom, can significantly accumulate lipids under nitrogen (N) limitation. However, the molecular mechanism underlying lipid accumulation in A. coffeaeformis remains unknown and its application development is lagging. Results: This work analyzed the lipid composition of A. coffeaeformis under N deprivation and investigated its mechanism underlying lipid accumulation using RNA-seq. The results showed that the total lipid content of A. coffeaeformis increased from 28.22 to 44.05% after 5 days of N deprivation, while the neutral lipid triacylglycerol (TAG) content increased from 10.41 to 25.21%. The transcriptional profile showed that N deprivation induced wide-ranging reprogramming of regulation and that most physiological activities were repressed, while the upregulation of glycerol-3-phosphate acyltransferase directly determined TAG accumulation. Moreover, we explored the effect of A. coffeaeformis as a food additive on the lipid composition of crucian carp. The results showed that the contents of unsaturated fatty acids in the meat of fish supplemented with A. coffeaeformis were significantly increased, indicating its potential application in animal nutrition for improving meat quality indicators. Conclusion: The findings shed light on the molecular mechanisms of neutral lipid accumulation and revealed the key genes involved in lipid metabolism in A. coffeaeformis. Moreover, we also confirmed that A. coffeaeformis can be used as feed additive for improving the lipid composition of crucian carp meat, which provided evidence for the biotechnology application of this high-oil microalgae. [ABSTRACT FROM AUTHOR]

Thlaspi arvense (Pennycress) is an emerging feedstock for biofuel production because of its high seed oil content enriched in erucic acid. A transcriptomic and a lipidomic study were performed to analyze the dynamics of gene expression, glycerolipid content and acyl-group distribution during seed maturation. Genes involved in fatty acid biosynthesis were expressed at the early stages of seed maturation. Genes encoding enzymes of the Kennedy pathway like diacylglycerol acyltransferase1 (TaDGAT1), lysophosphatidic acid acyltransferase (TaLPAT) or glycerol 3-phosphate acyltransferase (TaGPAT) increased their expression with maturation, coinciding with the increase in triacylglycerol species containing 22:1. Positional analysis showed that the most abundant triacylglycerol species contained 18:2 at sn-2 position in all maturation stages, suggesting no specificity of the lysophosphatidic acid acyltransferase for very long chain fatty acids. Diacylglycerol acyltransferase2 (TaDGAT2) mRNA was more abundant at the initial maturation stages, coincident with the rapid incorporation of 22:1 to triacylglycerol, suggesting a coordination between Diacylglycerol acyltransferase enzymes for triacylglycerol biosynthesis. Genes encoding the phospholipid-diacylglycerol acyltransferase (TaPDAT1), lysophosphatidylcholine acyltransferase (TaLPCAT) or phosphatidylcholine diacylglycerolcholine phosphotransferase (TaPDCT), involved in acyl-editing or phosphatidyl-choline (PC)-derived diacylglycerol (DAG) biosynthesis showed also higher expression at the early maturation stages, coinciding with a higher proportion of triacylglycerol containing C18 fatty acids. These results suggested a higher contribution of these two pathways at the early stages of seed maturation. Lipidomic analysis of the content and acyl-group distribution of diacylglycerol and phosphatidyl-choline pools was compatible with the acyl content in triacylglycerol at the different maturation stages. Our data point to a model in which a strong temporal coordination between pathways and isoforms in each pathway, both at the expression and acyl-group incorporation, contribute to high erucic triacylglycerol accumulation in Pennycress.

Obesity remains one of the largest health problems in the world, arising from the excess storage of triglycerides (TAGs). However, the full complement of genes that are important for regulating TAG storage is not known. The Glut1 gene encodes a Drosophila glucose transporter that has been identified as a potential obesity gene through genetic screening. Yet, the tissue-specific metabolic functions of Glut1 are not fully understood. Here, we characterized the role of Glut1 in the fly brain by decreasing neuronal Glut1 levels with RNAi and measuring glycogen and TAGs. Glut1RNAi flies had decreased TAG and glycogen levels, suggesting a nonautonomous role of Glut1 in the fly brain to regulate nutrient storage. A group of hormones that regulate metabolism and are expressed in the fly brain are Drosophila insulin-like peptides (Ilps) 2, 3, and 5. Interestingly, we observed blunted Ilp3 and Ilp5 expression in neuronal Glut1RNAi flies, suggesting Glut1 functions in insulin-producing neurons (IPCs) to regulate whole-organism TAG and glycogen storage. Consistent with this hypothesis, we also saw fewer TAGs and glycogens and decreased expression of Ilp3 and Ilp5 in flies with IPC-specific Glut1RNAi. Together, these data suggest Glut1 functions as a nutrient sensor in IPCs, controlling TAG and glycogen storage and regulating systemic energy homeostasis.

Despite the high diversity of elasmobranch fishes in the Balearic Islands, knowledge of their biology and population dynamics is still scarce. A recent mark-and-recapture experiment off the Balearic Islands tagged 3738 individuals of 23 shark and batoid species during MEDITS and CANAL bottom trawl scientific surveys from June 2021 to August 2023. Retrieval was reported for the sharks Scyliorhinus canicula and Mustelus mustelus, revealing relatively small home ranges for these species (0.2–38.5 km and 7.8–15.3 km for S. canicula and M. mustelus, respectively). Recapture efficiency was higher from scientific surveys than from commercial catches, highlighting potential challenges in collaboration with fishermen and recapture reports. Density estimates obtained from the MEDITS bottom trawl survey suggest a much larger population for S. canicula than estimates from the mark–recapture data, indicating MEDITS density estimates for this species may be overestimated due to its scavenger behavior perhaps favoring individuals searching for discards aggregated in the fishing grounds. This study emphasizes the importance of monitoring, collaborative efforts, and improved reporting mechanisms to enhance our understanding of elasmobranch populations and provide support for sustainable management of these vulnerable marine species.

Hashtags affect the conceptual sphere of Internet users and may promote certain ideologies because they are a synthetic tool of virtual communication. The research objective was to classify hashtags in virtual communication using the methods of conversational, contextual, discursive, content, and intent analyses. The study covered the online representations of the Kemerovo State University and its hashtags (2020–2023), namely, those of its official accounts, institutes, departments, trade unions, and student communities. The author revealed the following functions. The predictive function guided the first impression and was followed by compression, structuring, differentiating, and navigation functions, which systematized the content. The stereotyping function manifested itself in the image formation, while the axiological function asserted a set of ideologies behind it. The actualizing function was responsible for correlating the content with the media agenda. The manipulative and attractive functions demonstrated the suggestive aspect of the communicative potential of hashtags that represented the basic concepts of the university's conceptual sphere: kinship and family, community, a sense of belonging, stability, etc. In general, they developed an ideological model that formed a positive image of the university for the target audience.

Long-Range Wide-Area Networks (LoRaWAN) allow the transmission of data via radio link from sensors, which are potentially isolated or difficult to access, to gateways and servers that are connected to cellular networks for data processing, exchange, or relay, with low transmission power. This concept employs Long-Range (LoRa) modulation and has led to the emergence of many applications for the monitoring and tracking of objects. However, due to its characteristic of a low data rate for low-power communication, the transmission of information with LoRa technology is not suitable for the fast real-time monitoring of data. Additionally, due to its narrow bandwidth, an attempt to perform localization through the LoRa modulation technique will result in very limited accuracy because of its inability to resolve multipath problems. Thus, in this paper, we propose a multi-standard Ultra-Wide Bandwidth (UWB) and LoRa end-device that is capable of measuring location with high accuracy using UWB technology and then transmitting the location information through LoRa method to gateways and the Internet of Things Network. The results of measurements in indoor and outdoor scenarios show a UWB localization accuracy that is of sub-meter level, being between 10 and 33 cm, and a UWB range of 124 m in Line-of-Sight (LOS) and 55 m in Non-Line-of-Sight (NLOS) applications, respectively. [ABSTRACT FROM AUTHOR]

Purpose: As blunt thoracic aortic injury (BTAI) treatment has shifted from open to thoracic endovascular aortic repair (TEVAR), logistical challenges exist in creating and maintaining inventories of appropriately sized stent-grafts, including storage demands, shelf-life management and cost. We hypothesized that most injured aortas can be successfully repaired with a narrow range of stent-graft sizes and present a value-based anatomic approach to optimizing inventory. Methods: CT-scans of all patients with BTAI admitted to our Level I trauma center from Apr 2010-Dec 2018 were reviewed. Patients with anatomy incompatible with TEVAR were excluded. For each patient, after aortic sizing a set of two stent-grafts most likely to be utilized was selected from a list of twenty commercially available GORE conformable TAG endografts based on manufacturer instructions. Stent-graft sizes were then ranked based on the number of cases they would be suitable for. MATLAB was utilized to determine the combinations of stent-grafts which would cover the most patients. Results: Twenty-eight patients with BTAI were identified and three were excluded based on iliac diameter. Most patients were male (68%), mean age 42.3 ± 20.2 years, mean ISS 37.0 ± 9.8. Overall mortality was 25%. Of the 20 available stent-graft options, a combination of four stent-grafts would successfully treat 100% of the patients in this series. Conclusions: Based on actual CT-scan aortic measurements, we demonstrated that an inventory of four sent-graft sizes was sufficient to treat 100% of patients with BTAI. These data can be utilized as a value-based anatomic approach to aortic stent-graft institutional inventory creation and maintenance. [ABSTRACT FROM AUTHOR]

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Structured lipids (SLs) offer a promising avenue for designing novel formulations enriched in n-3 long-chain polyunsaturated fatty acids (LCPUFAs) with potential health benefits. Triacylglycerols (TAGs), the most common fats in the human diet, are both non-toxic and chemically stable. The metabolic efficiency and digestibility of TAGs are significantly influenced by the position of fatty acids (FAs) within the glycerol backbone, with FAs at the sn-2 position being readily absorbed. Over the past two decades, advancements in SL research have led to the development of modified TAGs, achieved either through chemical or enzymatic processes, resulting in SLs. The ideal structure of SLs involves medium-chain FAs at the sn-1,3 positions and long-chain n-3 LCPUFAs at the sn-2 position of the glycerol backbone, conferring specific physicochemical and nutritional attributes. These tailored SL formulations find wide-ranging applications in the food and nutraceutical industries, showing promise for dietary support in promoting health and mitigating various diseases. In particular, SLs can be harnessed as functional oils to augment TAG metabolism, thereby impeding the development of fatty liver, countering the onset of obesity, and preventing atherosclerosis and age-related chronic diseases. In scrutinising prevailing research trajectories, this review endeavours to provide an in-depth analysis of the multifaceted advantages and repercussions associated with the synthesis of SLs. It elucidates their burgeoning potential in enhancing health and well-being across a range of demographic cohorts. Specifically, the implications of SL utilisation are discussed in the context of healthcare environments and early childhood developmental support. [ABSTRACT FROM AUTHOR]

In recent years, virtual online communities have experienced rapid growth. These communities enable individuals to share and manage images or websites by employing tags. A collaborative tagging system (CTS) facilitates the process by which internet users collectively organize resources. CTS offers a plethora of useful information, including tags and timestamps, which can be utilized for recommendations. A tag represents an implicit evaluation of the user's preference for a particular resource, while timestamps indicate changes in the user's interests over time. As the amount of information increases, it is feasible to integrate more detailed data, such as tags and timestamps, to improve the quality of personalized recommendations. The current study employs collaborative filtering (CF), which incorporates both tag and time information to enhance recommendation precision. A computational recommender system is established to generate weights and calculate similarities by incorporating tag data and time. The effectiveness of our recommendation model was evaluated by linearly merging tag and time data. In addition, the proposed CF method was validated by applying it to big data sets in the real world. To assess its performance, the size of the neighborhood was adjusted in accordance with the standard CF procedure. The experimental results indicate that our proposed method significantly improves the quality of recommendations compared to the basic CF approach. [ABSTRACT FROM AUTHOR]

The marine microalga Nannochloropsis oculata has garnered significant interest as a potential source of lipids, both for biofuel and nutrition, containing significant amounts of C16:0, C16:1, and C20:5, n-3 (EPA) fatty acids (FA). Growth parameters such as temperature, pH, light intensity, and nutrient availability play a crucial role in the fatty acid profile of microalgae, with N. oculata being no exception. This study aims to identify key variables for the FA profile of N. oculata grown autotrophically. To that end, the most relevant literature data were gathered and combined with our previous work as well as with novel experimental data, with 121 observations in total. The examined variables were the percentages of C14:0, C16:0, C16:1, C18:1, C18:2, and C20:5, n-3 in total FAs, their respective ratios to C16:0, and the respective content of biomass in those fatty acids in terms of ash free dry weight. Many potential predictor variables were collected, while dummy variables were introduced to account for bias in the measured variables originating from different authors as well as for other parameters. The method of multiple imputations was chosen to handle missing data, with limits based on the literature and model-based estimation, such as using the software PHREEQC and residual modelling for the estimation of pH. To eliminate unimportant predictor variables, LASSO (Least Absolute Shrinkage and Selection Operator) regression analysis with a novel definition of optimal lambda was employed. LASSO regression identified the most relevant predictors while minimizing the risk of overfitting the model. Subsequently, stepwise linear regression with interaction terms was used to further study the effects of the selected predictors. After two rounds of regression, sparse refined models were acquired, and their coefficients were evaluated based on significance. Our analysis confirms well-known effects, such as that of temperature, and it uncovers novel unreported effects of aeration, calcium, magnesium, and manganese. Of special interest is the negative effect of aeration on polyunsaturated fatty acids (PUFAs), which is possibly related to the enzymatic kinetics of fatty acid desaturation under increased oxygen concentration. These findings contribute to the optimization of the fatty acid profile of N. oculata for different purposes, such as production of, high in PUFAs, food or feed, or production of, high in saturated and monounsaturated FA methyl esters (FAME), biofuels. [ABSTRACT FROM AUTHOR]

Background & aimsThe accumulation of neutral lipids within hepatocytes underlies non-alcoholic fatty liver disease (NAFLD), which affects a quarter of the world's population and is associated with hepatitis, cirrhosis, and hepatocellular carcinoma. Despite insights gained from both human and animal studies, our understanding of NAFLD pathogenesis remains limited. To better study the molecular changes driving the condition we aimed to generate a humanised NAFLD mouse model.MethodsWe generated TIRF (transgene-free Il2rg -/-/Rag2 -/-/Fah -/-) mice, populated their livers with human hepatocytes, and fed them a Western-type diet for 12 weeks.ResultsWithin the same chimeric liver, human hepatocytes developed pronounced steatosis whereas murine hepatocytes remained normal. Unbiased metabolomics and lipidomics revealed signatures of clinical NAFLD. Transcriptomic analyses showed that molecular responses diverged sharply between murine and human hepatocytes, demonstrating stark species differences in liver function. Regulatory network analysis indicated close agreement between our model and clinical NAFLD with respect to transcriptional control of cholesterol biosynthesis.ConclusionsThese NAFLD xenograft mice reveal an unexpected degree of evolutionary divergence in food metabolism and offer a physiologically relevant, experimentally tractable model for studying the pathogenic changes invoked by steatosis.Lay summaryFatty liver disease is an emerging health problem, and as there are no good experimental animal models, our understanding of the condition is poor. We here describe a novel humanised mouse system and compare it with clinical data. The results reveal that the human cells in the mouse liver develop fatty liver disease upon a Western-style fatty diet, whereas the mouse cells appear normal. The molecular signature (expression profiles) of the human cells are distinct from the mouse cells and metabolic analysis of the humanised livers mimic the ones observed in humans with fatty liver. This novel humanised mouse system can be used to study human fatty liver disease.

La floculación es un método de precipitación que retiene los sólidos suspendidos, liberando aguas grises con una carga orgánica menor. El polvo de moringa tiene un efecto floculante y en este caso se evaluó su efecto entre las poblaciones bacterianas, especialmente las de importancia en salud pública. El estudio se realizó en la Ciudad de Reynosa, Tamaulipas, que tiene dos canales que conducen agua para uso urbano y agrícola. Cuatro sitios fueron muestreados, dos en la ciudad y los otros en la periferia de la ciudad. Las muestras de agua recolectadas en cada punto fueron divididas en dos. Un lote fueron los controles y al otro se les agregó polvo de moringa. Posteriormente, en todos los lotes se realizó una extracción de ADN para análisis metagenómico de la biota presente. Los resultados mostraron que hay un efecto floculante en las muestras de agua y que con el tratamiento se alteran las poblaciones microbianas; finalmente, se observa que las poblaciones de bacterias de importancia en salud pública no son retiradas con el tratamiento.

At the Trans‐Atlantic Geotraverse hydrothermal field, metalliferous sediments cover extinct hydrothermal mounds and the surrounding seafloor. Here, we report the morphological, mineralogical and geochemical processes that deposit these sediments, remobilize their metals, and affect their preservation. We found that the initial sediment metal tenor is controlled by physical transport of hydrothermal material from its source, followed by diagenetic redistribution and potentially diffuse fluid flow after high‐temperature hydrothermal activity has ceased. We distinguished three different environments: (a) proximal metalliferous sediments on top of extinct mounds are mainly derived from oxidative weathering of primary sulfide structures and are predominantly composed of Fe oxyhydroxides with low contents of Cu, Co, and Zn; metal enrichments in specific layers are likely related to upward flow of low‐temperature hydrothermal fluids; (b) medial distant metalliferous sediments found at the base of the mounds, deposited by mass transport, contain cm‐thick layers of unsorted sulfide sands with high base metal contents (e.g., up to 28% Cu); these buried sulfides continue to undergo dissolution, resulting in metal release into porewaters; (c) distal metalliferous sediments, found in depositional basins a few hundreds of meters from the extinct mounds, include fining‐upwards sequences of thin sulfide sand layers with Fe oxyhydroxides and were deposited by recurrent turbiditic flows. Dissolved metals (e.g., Cu2+ and Mn2+) diffuse upwards under reducing conditions and precipitate within the sediment. Hence, when using hydrothermal sediments to construct reliable geochronological records of hydrothermal activity, distance from source, local seafloor morphology, mass‐transport and depositional, and diagenetic modification should all be considered. Plain Language Summary: The seafloor massive sulfide mounds at the TAG hydrothermal field (Mid‐Atlantic Ridge) are surrounded by metalliferous sediments. These sediments can be used to understand the history of the hydrothermal activity; however, the evolution of these sediments can be complex. The aim of this study is to understand the processes that lead to the accumulation and alteration of these sediments. For this, we identified the mineralogical, geochemical and physical processes that affect the sediments. Our results show that sediments found on mound tops are mainly composed of iron and manganese oxides and hydroxides, and are low in other metal contents compared to other environments. Medial distant sediments at the base of the mounds have thick beds of sulfide‐rich sediments that contain the highest concentrations of metals and are currently undergoing dissolution. In depositional channels, several hundreds of meters distal from the mounds, metalliferous sediment have accumulated in several meters thick layers that contain high concentrations of dissolved metals. This study reveals that the metal content of hydrothermal sediments is strongly controlled by their physicochemical environment and processes after deposition, and we suggest these processes should be considered when using metalliferous sediments as records of the evolution and activity of hydrothermal systems. Key Points: TAG hydrothermal field sediments have high metal contents that vary distinctly between different seafloor depositional environmentsLowest metal contents are found in sediments on top and highest in those at the base of extinct hydrothermal moundsDeposition, remobilization, and preservation of metals in hydrothermal sediments lead to distinctively different sediment characteristics [ABSTRACT FROM AUTHOR]

Choosing a code when interacting is one of the opportunities that a bilingual has. Code-switching is usually used by bilinguals when interacting with the other person. This study tries to find the types of code-switching and the categories of reasons for using code-switching in lecturer- students’ interactions in thesis exams. This research used a qualitative method with a case study approach. The data was in the form of utterances obtained from recorded interactions of five thesis examination sessions in the English undergraduate program at Syiah Kuala University. The data was analyzed through several processes, including transcribing, data condensation, identifying, marking, grouping, displaying data, and making inferences. The data was analyzed using the theory proposed by Stockwell and Hoffman. From the analysis, it was found that there are three types of code-switching, namely tag switching, inter-sentential, and intra-sentential. Related to the categories of reasons for using code-switching, the researcher found 5 from 7 reasons to do code-switching in lecturer-students’ interaction in thesis examination. The researcher hoped that the next researcher will further analyze the code-switching that occurs in the thesis exam, not only analyzing the types of code-switching and the reasons for using code-switching, but also analyzing the other side of linguistics.

RESUMO Objetivo Medir a prevalência de sintomatologia ansiosa entre estudantes de graduação de uma universidade pública no Sul do Brasil, seus fatores associados e possíveis consequências. Métodos Trata-se de um estudo transversal com uma amostra aleatória de graduandos, realizado em 2019. Foi utilizado um questionário com questões sociodemográficas, acadêmicas e de saúde mental e física. Sintomas de ansiedade generalizada foram avaliados por meio do Generalized Anxiety Disorder – 7 (GAD-7). Fatores associados e possíveis consequências foram analisados por meio da regressão de Poisson com ajuste robusto da variância. Resultados Entre os 994 participantes, a prevalência de sintomas de ansiedade generalizada severa foi de 30,9% (IC 95%: 27,9%-33,9%). As variáveis independentemente associadas a sintomas de ansiedade generalizada foram: sexo feminino, orientação não heterossexual, sentir muito/muitíssimo medo de violência no bairro e perceber como difícil o acesso a serviço psicológico. Possuir alto nível de suporte social e apresentar algum nível de atividade física foi considerado fator de proteção para o desfecho. Possíveis consequências dos sintomas de ansiedade generalizada foram: pior qualidade de sono, utilizar algum medicamento sem prescrição nos últimos 30 dias e ser classificado como risco de suicídio moderado/severo. Conclusões Esses resultados demonstram a necessidade de um olhar mais atento para alguns subgrupos populacionais. Recomenda-se a implementação de intervenções psicológicas que incluam um número maior de indivíduos simultaneamente (como a criação de grupos terapêuticos), com o intuito de reduzir a carga de ansiedade no contexto universitário. O fortalecimento do setor de assistência estudantil da universidade também se torna uma importante possibilidade de melhora para esse cenário.