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伴有破骨样巨细胞的胰腺未分化癌（UCPOGC）是胰腺导管腺癌的一种罕见的特殊亚型。肿瘤成分复杂，其中可见3种细胞类型：肿瘤细胞、单核组织细胞和破骨样巨细胞。肿瘤细胞异型性显著，通常不表达或仅灶性表达上皮标志物，而弥漫表达间叶源性标志物波形蛋白，因此常被误诊为肉瘤；后两者表达组织细胞标志物，缺乏上皮分化。现报道2例UCPOGC，分析临床病理学特征，并查阅相关文献，重点讨论其组织起源及预后相关因素，以提高临床与病理医师对该肿瘤的认识水平。.

We present photometric and spectroscopic data on three extragalactic luminous red novae (LRNe): AT 2018bwo, AT 2021afy, and AT 2021blu. AT 2018bwo was discovered in NGC 45 (at about 6.8 Mpc) a few weeks after the outburst onset. During the monitoring period, the transient reached a peak luminosity of 1040 erg s−1. AT 2021afy, hosted by UGC 10043 (∼49.2 Mpc), showed a double-peaked light curve, with the two peaks reaching a similar luminosity of 2.1(±0.6)×1041 erg s−1. Finally, for AT 2021blu in UGC 5829 (∼8.6 Mpc), the pre-outburst phase was well-monitored by several photometric surveys, and the object showed a slow luminosity rise before the outburst. The light curve of AT 2021blu was sampled with an unprecedented cadence until the object disappeared behind the Sun, and it was then recovered at late phases. The light curve of LRN AT 2021blu shows a double peak, with a prominent early maximum reaching a luminosity of 6.5 × 1040 erg s−1, which is half of that of AT 2021afy. The spectra of AT 2021afy and AT 2021blu display the expected evolution for LRNe: a blue continuum dominated by prominent Balmer lines in emission during the first peak, and a redder continuum consistent with that of a K-type star with narrow absorption metal lines during the second, broad maximum. The spectra of AT 2018bwo are markedly different, with a very red continuum dominated by broad molecular features in absorption. As these spectra closely resemble those of LRNe after the second peak, AT 2018bwo was probably discovered at the very late evolutionary stages. This would explain its fast evolution and the spectral properties compatible with that of an M-type star. From the analysis of deep frames of the LRN sites years before the outburst, and considerations of the light curves, the quiescent progenitor systems of the three LRNe were likely massive, with primaries ranging from about 13 M⊙ for AT 2018bwo, to 14−1+4 M⊙ for AT 2021blu, and over 40 M⊙ for AT 2021afy.

We present an observational study of the luminous red nova (LRN) AT 2021biy in the nearby galaxy NGC 4631. The field of the object was routinely imaged during the pre-eruptive stage by synoptic surveys, but the transient was detected only at a few epochs from ∼231 days before maximum brightness. The LRN outburst was monitored with unprecedented cadence both photometrically and spectroscopically. AT 2021biy shows a short-duration blue peak, with a bolometric luminosity of ∼1.6 × 1041 erg s−1, followed by the longest plateau among LRNe to date, with a duration of 210 days. A late-time hump in the light curve was also observed, possibly produced by a shell-shell collision. AT 2021biy exhibits the typical spectral evolution of LRNe. Early-time spectra are characterised by a blue continuum and prominent H emission lines. Then, the continuum becomes redder, resembling that of a K-type star with a forest of metal absorption lines during the plateau phase. Finally, late-time spectra show a very red continuum (TBB ≈ 2050 K) with molecular features (e.g., TiO) resembling those of M-type stars. Spectropolarimetric analysis indicates that AT 2021biy has local dust properties similar to those of V838 Mon in the Milky Way Galaxy. Inspection of archival Hubble Space Telescope data taken on 2003 August 3 reveals a ∼20 M⊙ progenitor candidate with log (L/L⊙) = 5.0 dex and Teff = 5900 K at solar metallicity. The above luminosity and colour match those of a luminous yellow supergiant. Most likely, this source is a close binary, with a 17–24 M⊙ primary component.

Temperature is one of the important parameters that effect the production of metal ion using MIVOC method. Beams of highly charged iron ions are produced with 14.5 GHz all-permanent-magnet electron cyclotron resonance ion source at three temperatures at 320 kV high voltage multi-discipline platforms for highly charged ions at IMP, CAS. At 18 °C, Fe13+ and Fe15+ of more than 15.9 eμA and 11.7 eμA have been extracted with an extraction voltage of 15 kV and a microwave power of 300 W. When increasing temperature to 25 #x00B0;C and 30 #x00B0;C, more main substance arrives at the plasma chamber of ion source and the outputs of Fe15+ and Fe16+ decreases, but those of Fe13+, Fe12+ and Fe11+ increase, the distribution of Fe ions shifts towards low charge state. The conductance of the coupled MIVOC system is estimated at different temperatures. The ionization efficiencies and the effective consumptions rate of ferrocene are also figured out from the vapour pressure and the intensities of iron ion beams. With the increase of the temperature, the conductance and the consumptions increases while the dependence of ionization efficiency on the temperature is different.

The corneal allograft rejection is the primary reason for graft failure, but the existing agents are of limited efficacy and may be accompanied by unacceptable morbidity. Recently, antibody-based agents have received great attention and have become an important part of therapeutic intervention for organ transplantation, which is also a research focus in the field of corneal transplantation. This review summarizes the history, current situation and mechanism of antibody-based agents in corneal transplantation.

We report the photometry of six transits of the hot Jupiter HAT-P-29b obtained from 2013 October to 2015 January. We analyze the new light curves, in combination with the published photometric, and Doppler velocimetric, and spectroscopic measurements, finding an updated orbital ephemeris for the HAT-P-29 system, $T_{\rm C}[0]= 2456170.5494(15)\,[\rm{BJD_{TDB}}]$ and $P=5.723390(13) \,{\rm days}$. It is $17.63\,{\rm s}$ ($4.0\,\sigma$) longer than the previously published value, amounting to errors exceeding $2.5\,\rm{hrs}$ at the time of writing (on UTC 2018 June 1). The measured transit mid-times for HAT-P-29b show no compelling evidence of timing anomalies from a linear model, which rules out the presence of a perturbers with masses greater than $0.6$, $0.7$, $0.5$, and $0.4\,{\rm M_\oplus}$ near the $1:2$, $2:3$, $3:2$, and $2:1$ resonances with HAT-P-29b, respectively., Comment: Accepted for publication in AJ

The prognostic role of phosphatase and tensin homolog (PTEN) in non-small cell lung cancer (NSCLC) has been controversial.In this study, levels of PTEN expression were investigated in NSCLC patients and their prognostic value in NSCLC was assessed. PTEN expression in tumor tissues from 68 NSCLC patients was analyzed using immunohistochemistry and confocal microscopy. Survival analysis was performed using the log-rank test and Cox proportional hazards regression analysis.NSCLC patients classified as expressers of high levels of PTEN (n = 46) had better prognoses than those classified as expressers of low levels (mean survival 17.1 versus 12.9 months, log-rank p = 0.038). In patients with adenocarcinoma (AC), high PTEN expression (n = 9) was associated with significantly longer survival than low PTEN expression (mean survival 23.50 versus 15.54 months, log-rank p = 0.043). High levels of PTEN expression resulted in 43% reduction in risk for all NSCLC patients (HR = 0.57, 95% CI: 0.33-0.98, p = 0.041). PTEN expression and clinical stage remained significantly associated with survival after adjustment for age, sex and tumor type (HR = 0.56, 95% CI: 0.32-0.99; p = 0.048; HR = 0.54, 95% CI: 0.36-0.97; p = 0.045). No significant difference in continuous PTEN expression levels was observed among groups with different clinical or pathological characteristics (p0.17). When levels of PTEN expression were binarized using the optimal cutpoint, higher levels of PTEN expression were observed in patients with T1/T2 than in those with T3/T4 (80% and 58% respectively, p = 0.049) and in patients with AC than in those with squamous-cell carcinoma (SCC) (78% and 58% respectively, p = 0.08). No significant difference in binarized PTEN expression levels was found among groups with any other clinical/pathologic characteristic (p0.28).Our results suggest that high levels of PTEN expression may be favorable prognostic marker in NSCLC patients.

This study considers the compensation of water diversion projects for the values of the terrestrial biological resources, water environment, and aquatic biological resources in water sources. An analysis of capital dynamics was conducted, and the economic development coefficient was used to correct the current method for calculating ecological compensation. A model was constructed to calculatethe ecological compensation for the water sources for water diversion projects. This model was used to calculate the ecological compensation for the Niulanjiang River provided by the Niulanjiang River to the Dianchi Lake water diversion project, which was calculated to be 136,799,400 RMB. As long as we know the occupying area of the project, the change of the river net flow after diversion and the local average GDP, the ecological compensation for water sources could be calculated by the model. The proposed model for calculating the ecological compensation for water sources is simple and incorporates the compensation provided by water diversion projects for the various environmental effects on water sources. It provides a guarantee for the capital to be used for the environmental protection of water sources and facilitates the sustainable development of the ecological environments of water sources.

An osteoarthritis (OA) model was created in the knees of rabbits by injecting them with 0.3 mL of sterile papain solution in order to evaluate the effects of a hyaluronic acid (HA) and low molecular weight heparin (LMWH) injection on osteoarthritis. HA-LMWH, LMWH, and HA were injected into animals once weekly. After 5 weeks of treatment, the animals were sacrificed and the effects of the injections on osteoarthritis were evaluated by histological assessment. HA levels in cartilage and the levels of IL-1β and TNF-α expression in synovial fluids were determined. As shown by histological observation, recovery of the synovium and cartilage of animals injected with HA-LMWH was better than that in animals injected with HA or LMWH. HA levels in cartilage of animals injected with HA-LMWH were much higher than those of the control group. The levels of IL-1β expression in synovial fluids of animals injected with HA-LMWH were lower than those in other animals. The levels of TNF-α expression in synovial fluids of animals injected with HA-LMWH were much lower than those in the controls. In conclusion, HALMWH injection had a favorable anti-inflammatory and therapeutic effect on experimental OA.

To study the effect of menadione (Men) reducing doxorubicin (Dox) resistance in Ehrlich ascites carcinoma (EAC) cells resistant to Dox (EAC/Dox cells).Glutathione (GSH) content and membrane fluidity were measured by fluorometric assay and fluorescence depolarization assay, respectively. Glutathione S-transferase (GST) activity was measured with 1-chloro-2,4-dinitrobenzene as the substrate. Cell viability was determined by 3-(4, 5-dimethylthiazol)-2, 5-diphenyltetrazolium bromide assay.GSH content, GST activity, and membrane fluidity in EAC/Dox cells were higher than those in EAC cells (P0.01). The IC50 (95% confidence limits) for Dox on EAC/Dox cell was 22.3 (15.8-28.8) mg.L-1. Relative resistance of Dox in EAC/Dox cells was 42-fold. Pretreatment of EAC/Dox cells with Men 5 or 10 mg.L-1 decreased intracellular GSH content (P0.01). Men 1 mg.L-1 had no obvious effect on GSH content in EAC/Dox cells (P0.05), but decreased the elevated membrane fluidity efficiently (P0.05). Men had no obvious effect on GST activity in EAC/Dox cells (P0.05). IC50 of Dox was reduced to 9.6 (7.8-11.3), 6.0 (2.8-9.2), or 5.3 (3.9-6.7) mg.L-1 in EAC/Dox cells pretreated with Men 1, 5, or 10 mg.L-1.Men reduced Dox resistance effectively due in part to its depletion of GSH content in EAC/Dox cells.

In rats injected with bacterial lipopolysaccharide (LPS; 5 gamma mg/g body weight [BWT]), the toxin provokes death within 24 h in 23% of the animals and, in surviving rats, causes a decrease in BWT, hyperlactacidemia, hyperlipacidemia, and hyperketonemia, as well as depletion of both liver and muscle glycogen content. In the liver, LPS severely lowers the ATP and total adenine nucleotide content, ATP/ADP ratio, and adenylate charge. In hepatocytes from LPS-injected rats, the oxidation of D-glucose is first increased 2 h after administration of the toxin, despite close-to-normal phosphorylation of the hexose. In hepatocytes prepared from rats killed 24 h after injection of LPS, the phosphorylation of D-glucose, its incorporation into glycogen, and its oxidation are all severely impaired. This sequence of changes, which coincides with a decreased ratio between pyruvate and lactate production from exogenous D-glucose, is comparable to that found with agents that uncouple oxidative phosphorylation. The injection of LPS also alters the metabolic response of hepatocytes to the dimethyl ester of succinic acid (SAD), in terms, for instance, of the sparing action of the ester upon both the production of 14CO2 by hepatocytes prelabeled with L-[U-14C] glutamine and the output of NH4+, and its inhibitory action on glycogenolysis and futile cycling in the reactions catalyzed by glucokinase and glucose-6-phosphatase. Nevertheless, the infusion of SAD protects the rats against the deleterious effect of LPS upon such variables as the plasma concentration of free fatty acids and beta-hydroxybutyrate, the liver ATP content, and the oxidation of D-glucose, as well as the pyruvate/lactate ratio, in hepatocytes prepared from the LPS-injected rats. The infusion of SAD also virtually suppresses lethality in the LPS-injected animals. It is proposed, therefore, that the infusion of succinic acid esters may represent a novel therapeutic approach in endotoxemia and multiple-organ failure.

The methyl esters of succinic acid are potent insulin secretagogues, currently under investigation as possible tools in the treatment of non-insulin-dependent diabetes. The in vivo administration of these esters may result, however, in the undesirable generation of methanol. The present study reveals that other esters of succinic acid, such as the monoethyl, monopropyl, monoisopropyl, monoallyl and diallyl esters, stimulate insulin release when administered intravenously in a dose of 2 mumol/g body weight to anaesthetized fed rats. This indicates that several succinic acid esters, that are not susceptible to lead, through their intracellular hydrolysis, to the production of methanol remain efficient in vivo as insulin secretagogues.

UDP-glucose pyrophosphorylase was measured in rat pancreatic islets, the generation of D-glucose 1-phosphate from UDP-glucose and PPI being eventually coupled to the generation of L-[U-14C]glutamate from 14C-labelled alpha-ketoglutarate. The activity of the enzyme was about one order of magnitude lower in islet than liver homogenates. The affinity of the enzyme for either UDP-glucose or PPi was comparable, however, in liver and islets. The activity of UDP-glucose pyrophosphorylase was somewhat lower in islets from animals with inherited or acquired diabetes mellitus than in those from control rats. These findings are considered in connection with the accumulation of glycogen in islets of hyperglycemic animals.

Using 3-(4,5-dimethythiazole)-2,5-diphenyltetrazolium bromide (MTT) method, the effect of probimane (Pro) on doxorubicin (Dox) cytotoxicity was studied. Pro 0.313, 0.625, and 1.25 micrograms.ml-1 potentiated cytotoxicity of Dox in Ehrlich ascites carcinoma (EAC) cells. Incubation of EAC cells with Dox 10 micrograms.ml-1 and Pro 116.5, 233, and 466 micrograms.ml-1 resulted in an increase in intracellular drug accumulation from 0.69 +/- 0.06 to 1.08 +/- 0.10 micrograms/10(7) cells. In S37-bearing mice, Pro 23.3, 46.6, and 116.5 micrograms.ml-1 enhanced the malondialdehyde (MDA) formation in tumor and liver mitochondria and decreased MDA formation in liver mitochondria. These results suggested that the increases of Dox accumulation and MDA formation in tumor cells by Pro might be the reasons for synergistic effect of Pro on Dox cytotoxicity.

Succinic acid methyl esters are currently under investigation as potential insulinotropic tools in animal models of non-insulin-dependent diabetes mellitus. The in vivo administration of these esters may result in the undesirable generation of methanol through their intracellular hydrolysis. As a first attempt to circumvert this drawback, we have now investigated whether the esterification of the carboxylic group of succinic acid monomethyl ester by D-glucose or 3-O-methyl-D-glucose affects its insulin-otropic action. Both the 6-O-D-glucosyl and 6-O-(3-O-methyl)-D-glucosyl esters were found to stimulate insulin release in pancreatic islets and the isolated perfused pancreas. The 6-O-D-glucosyl ester also stimulated insulin release after intravenous administration to anaesthetized rats. These findings suggest that the undersirable generation of methanol from the methyl esters of succinic acid could eventually be avoided by using other esters of this dicarboxylic acid, whilst keeping the benefit of their insulinotropic action.

Determination of GSH was performed by a modified fluorometric method. The experiments showed that the GSH content in resistant cell line (EAC/ADR) was 4 fold higher than that in sensitive cell line (EAC), being 1.25 +/- 0.35 microgram/10(6) cells in EAC/ADR and 0.31 +/- 0.12 microgram/10(6) cells in EAC. When the drug treatment was discontinued for 36 weeks, the sensitivity was partially recovered and the GSH level in EAC/ADR(R) cell parallelly decreased. In addition, vincristine, mitomycin C, actinomycin D and VP-16 showed no effect on the GSH content in the EAC/ADR cell.

Qualitative and quantitative analyses of the essential oil steamdistilled from the aerial parts of Ligusticum chuanxiong were made by means of GC-MS and GC. Forty-six components which make up 85.82% of the total oil were identified.

We analysed the early viremia and clinical tests in 82 patients with epidemic hemorrhagic fever (EHF). The results showed that the changes in viremia and clinical tests are related to the severity of the disease and prognosis. Higher concentrations of the virus in infected patients might cause a more unfavourable prognosis and more abnormalities in clinical tests. CK-MB, SGOT, SGPT, serum creatinine and urea nitrogen contents increased markedly, while serum total protein, albumin and calcium contents decreased markedly, indicating that the heart, liver and kidney in EHF patients were severely damaged. Markedly increased WBC and monocytes showed that the patients were seriously infected. Platelet count, antithrombin-III and plasminogen decreased markedly, demonstrating that there were marked changes in the coagulation-anticoagulation and fibrinolytic system of the EHF patients. Changes in RBC, Hb and HCT contents indicated that the blood in the EHF patients had a higher concentration. This study gives further evidence that EHFV plays an important role in the pathogenesis of EHF.

The isolated rat liver mitochondria were used in vitro to study the effect of doxorubicin on lipid peroxidation. We found that NADH-dependent mitochondrial peroxidation, measured by thiobarbituric acid (TBA) method, was stimulated to fourfold by doxorubicin (50 mumol.L-1). The addition of Fe3+ produced a significant increase of malondialdehyde (MDA) formation induced by doxorubicin. Doxorubicin enhanced the peroxidation of lipids in liver mitochondria through enzymatic mechanism especially in the presence of Fe3+. Probimane, bimolane, dexrazoxane (dioxopiperazine compounds), and edetic acid (EDTA) inhibited the formation of MDA in doxorubicin or doxorubicin+FeCl3 systems in a concentration-dependent manner. The inhibitory rates of MDA formation by probimane at the concentrations of 0.01, 0.05, 0.1, and 0.25 mmol.L-1 were 27.80%, 25.19%, 47.80%, and 59.77% respectively; bimolane were 21.04%, 25.55%, 24.83%, and 54.13%; dexrazoxane were 11.29%, 20.68%, 34.94%, and 58.65%; EDTA were 57.52%, 55.67%, 61.62%, and 63.16% in Dox and FeCl3 system. The inhibitory rates of MDA formation by probimane at concentration 0.01, 0.05, 0.1, and 0.25 mmol.L-1 were 19.27, 39.02, 59.60, and 58.63% respectively; bimolane were 6.10, 17.19, 41.58, and 53.22%; dexrazoxane were 27.24, 33.26, 58.21, and 59.11%; EDTA were 63.76, 67.43, 61.68, and 63.27% respectively in Dox system. These results suggested that protection against cardiotoxicity of doxorubicin afforded by probimane, bimolane, and dexrazoxane may be related to their ability to combine with the complex iron so that the iron was no longer able to take part in free radical reactions.

The effectiveness of adriamycin (ADR) in the treatment of cancer has been limited by the development of drug resistance. Verapamil and ligustrazini were found to show significant potentiating effect on the cytotoxicity of ADR in vitro and decreased the IC50 of ADR by 84.85% and 30.82% of the resistant line, respectively, and no effect was found on the IC50 in the sensitive line. The Glutathione-S-transferase activity of ADR-resistant and sensitive mouse EAC was determined in vivo. The activity in resistant cancer was 1.72-fold higher than that in sensitive cancer. Administration of verapamil or ligustrazini decreased the GST activity of resistant EAC by 35.50% or 26.05%, respectively and no effect was observed on sensitive cancer. The results show that verapamil and ligustrazini can reverse partly the ADR-resistance in resistant mouse EAC.

The effect of schisandrin B (Sin B) on oxygen free radicals--induced lipoperoxidative damage to plasma membrane of rat hepatocytes was investigated. When the plasma membrane of rat hepatocytes was incubated with iron/cysteine or Vit C/NADPH, the production of malondialdehyde (MDA) and consumption of NADPH increased, while the membrane fluidity reduced. Addition of Sin B (3-25 micrograms.ml-1) to the incubation mixture inhibited all these alterations of the plasma membrane induced by iron/cysteine and Vit C/NADPH. The results indicated that Sin B could maintain membrane stability of rat hepatocytes under oxidative stress.

Secondary derivative differential pulse polarography was developed and used for the quantitative analysis of ribostamycin sulfate, kanamycin and their preparations. By using sodium hydroxide-potassium dihydrogen phosphate solution as the base solution, ribostamycin sulfate and kanamycin showed good peaks at 0.105 V and -0.37 V (vs Ag/AgCl) respectively. The linear relationship of ribostamycin sulfate and kanamycin between concentration and peak height was obtained in the concentration range of 1.8-10.8 x 10(-4) mol/L and 3.1-18.6 x 10(-4) mol/L and the determination limit was 7.2 x 10(-9) mol/L and 4.6 x 10(-8) mol/L respectively. This method is simple, rapid and sensitive. The result is accurate.

Kinetic changes of viremia were observed in 287 cases of hemorrhagic fever with renal syndrome (HFRS) in whom ribavirin was administered with double blind random control studied by means of virus isolation, indirect immunofluorescence assay and enzyme-linked immunosorbent assay. The positive rate of viremia was 79.7% (Sp = 3%) and positive rate of HERS IgM was 85% (Sp = 3.1%) before treatment. Viremia could be interrupted by ribavirin as in the ribavirin treated group, the viremia positive rate decreased, duration of viremia was shortened, viral antigen products, virus titer and HFRS IgG antibody level were reduced as compared with the control group. This showed that viremia was very frequent in patients in the febrile phase and ribavirin is an effective antiviral drug in HFRS during the febrile phase. Dosage and course of treatment of this drug are discussed.

The combined techniques of x-ray-absorption fine structure and x-ray diffraction have been used to study the strain and bond distortions in epitaxial Ge-Si on Si(001). In a 31% Ge, 340-\AA{} pseudomorphic Ge-Si film, the Ge-Ge and Ge-Si first-neighbor bond lengths have been found to be 2.44\ifmmode\pm\else\textpm\fi{}0.02 and 2.38\ifmmode\pm\else\textpm\fi{}0.02 \AA{}, respectively. The lattice parameter perpendicular to the Ge-Si/Si(001) interface has been found to be ${\mathit{a}}_{\mathrm{\ensuremath{\perp}}}$=5.552\ifmmode\pm\else\textpm\fi{}0.002 \AA{}, in agreement with the predictions of macroscopic elastic theory. These results show that the bond-length strain in the epitaxial layer appears in the second and higher coordination shells, rather than in the nearest-neighbor bond lengths, which remain the same as in unstrained Ge-Si. A microscopic model is presented that accounts for these findings.

Probimane, dl-bis (4-morpholin-methyl 3,5-dioxopipweazin-1-yl) propane first synthesized in China, is a dioxopiperazin compound with antineoplastic, antimetastatic and radiopotentiating activities. In order to evaluate the mechanisms of cardiotoxicity protective action of probimane, the free radical induced by doxorubicin were analysed by electron spin resonance (ESR) techniques. Our studies showed that doxorubicin stimulated the formation of semiquinone free radicals in the rat heart homogenate and heart cell mitochondria systems, and probimane inhibited the free radical formation in both systems, with the dose-dependent and time-dependent responses. The inhibitory rates of doxorubicin free radical formation in rat heart homogenate system by probimane 0.6 mmol.L-1 at time of 3, 5, 10, 15, 30, 45 and 60 min were 44.6%, 43.0%, 51.5%, 74.3%, 68.1%, 56.1% and 39.9% respectively. The inhibitory rates of semiquinone free radical formation in mitochondria system by probimane at the concentration of 0.02, 0.06, 0.6, 1.2 and 2.4 mmol.L-1 were 17.07%, 29.87%, 63.95%, 64.62% and 83.64%, respectively. Probimane had no effect on NADH2, but inhibited NADH dehydrogenase activity at higher concentration.

The immunologic phenotypes of 12 cases of nonepidermotropic cutaneous T-cell lymphoma are reported. All cases consisted of mature peripheral T-cells. There were 9 cases of helper T-cell (Th) phenotype and 2 cases of suppressor T cell (Ts) phenotype. One case, however, expressed both the Th and Ts phenotypes. Two separate cases displayed OKT9 positivity. OKT6 positive lymphoid cells were found in the dermis during the early and late stages of the disease. We suggest that these OKT6 positive cells should represent a dermal infiltrate of Langerhans cells of immature T-cells.

Triptolide (Tri) is an active principle of Tripterygium hypoglaucum Hutch, which has been used to treat cancers and autoimmune diseases in folk medicine. The lymphocyte proliferation (LP) induced by Con A or Lipopolysaccharide (LPS) was suppressed by Tri at 50 and 500 ng/ml. At 5 ng/ml the suppressive effect on LP induced by Con A was much stronger than that by LPS. The humoral immune reaction monitored by quantitative hemolysis spectrophotometry (QHS) was suppressed by Tri 0.75 and 1 mg/kg ip. IL-2 production by mouse spleen cells were suppressed by Tri 5, 50,500 ng/ml in vitro and 0.5, 0.75 and 1 mg/kg ip. IL-2 activity was assayed by assessing its ability to support proliferation of selected IL-2-dependent T cell line, measured by colorimetric method which is based on the ability of viable cells to cleave MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide). Ts cells induced by supraoptimal immune (SOI) were suppressed by Tri 0.5 and 0.75 mg/kg ip. These results suggest T or its subpopulations and B lymphocytes may be the target cells of Tri.

Probimane, dl-1,2-bis (4-morpholine-methyl-3, 5-dioxopiperazin-1-yl) propane, is a new antitumor agent synthesized by Shanghai Institute of Materia Medica, Chinese Academy of Sciences. The scavenging effects of probimane on active oxygen radicals produced in 3 different systems were studied with the ESR spin trapping methods. In Fenton's reaction, probimane remarkably scavenged hydroxyl radicals (.OH) and the rate of scavenging .OH by probimane 0.05 mmol/L was 47%, compared to 5% by vitamin E (VE) and 30% by ascorbic acid (AA). In irradiation riboflavin system, in which superoxide (O2-.) was produced, the agent also had the scavenging effects on O2(-.). The rate of scavenging O2-. by probimane 0.05 mmol/L was 13%, higher than that by VE (7%) but lower than that by AA (90%). In cell system where the active oxygen radicals were produced during the respiratory burst of human neutrophils (Neu) stimulated by TPA (tetradecanoylphorbol acetate), probimane exhibited a dose-dependent scavenging action on the radicals. The rate of the radical scavenging by probimane 0.05 mmol/L was 37%, much higher than that by VE (9%) but lower than that by AA (68%). Probimane had no effect on the rate of oxygen consumption by human Neu, measured with spin probe oxymetry.

The action of schizandrin B (Sin B) was observed in freshly isolated hepatocytes damaged by FeSO4/cysteine and CCl4. Two types of free radicals, .OH and .CCl3, generated from FeSO4/cysteine and CCl4, respectively, induced lipid peroxidation in hepatocytes. It was found that the speed of lipid peroxidation (MDA production) and the degree of alteration in hepatocyte morphology were closely related to the type of free radicals. MDA production and membrane protrusion of hepatocytes injuries by FeSO4/cysteine were faster and more severe than those observed with CCl4. Sin B was shown to decrease the production of MDA and the release of GPT and LDH, and to increase hepatocyte viability as well as maintaining the integrity of the hepatocyte membrane surface. These actions of Sin B were stronger than vitamin E at the same concentration. It was observed that no inhibitory effect of phenobarbital, a typical inducer of cytochrome P-450, as Sin B induced liver cytochrome P-450, on MDA production in hepatocytes damaged by FeSO4/cysteine. The results suggest that Sin B possesses antioxidant activity.