1. Immunogenicity of a peptide-based vaccine for measles: a pilot evaluation in a mouse model.
- Author
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Quach, Huy Quang, Ratishvili, Tamar, Haralambieva, Iana H., Ovsyannikova, Inna G., Poland, Gregory A., and Kennedy, Richard B.
- Subjects
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MEASLES vaccines , *VACCINE immunogenicity , *LABORATORY mice , *HLA histocompatibility antigens , *ANIMAL disease models , *T cell receptors - Abstract
Although neutralizing antibody is an established correlate of protection for measles, T cell-mediated responses play at least two critical roles in immunity to measles: first, through provision of 'help' enabling robust humoral immune responses; and second, through clearance of measles virus-infected cells. Previously, we identified 13 measles-derived peptides that bound to human leukocyte antigen (HLA) molecules in Priess cells infected with measles virus. In this study, we evaluated the immunogenicity of these peptides in a transgenic mouse model. Our results demonstrated that these peptides induced Th1-biased immune responses at varying levels. Of the 13 peptides, the top four immunogenic peptides were further selected for a viral challenge study in mice. A vaccine based on a combination of these four peptides reduced morbidity and weight loss after viral challenge compared to placebo. Our results emphasize the potential of T cell-mediated, peptide-based vaccines against measles. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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