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Amaç: Çalışmamızın amacı, kalp ve akçiğer doku homojenatlarında malondialdehit (MDA) ve advanced oxidation protein products (AOPP), catalase (CAT), glutathione (GSH) ve glutathione peroxidase (GSH-Px) düzeylerinin farelerde deneysel PTZ-kindling modelinde oluşan nöbetlere karşı etkisi araştırılmıştır.Gereç ve Yöntem: Deneyde Swiss albino erkek fareler (n=42) kullanıldı. Hayvanlar Kontrol, pentilenetetrazol 35 mg/kg (PTZ) , valproat 100 mg/kg (VPA), artemisinin 30 mg/kg (ARS),+ PTZ, ARS 60 mg/kg+PTZ, ARS 120 mg/kg+PTZ olmak üzere altı gruba ayrıldı. Deneyin 26. gününde farelrer sakrifiye edilerek, Biyokimyasal analizler için numuneler -80 0C'de muhafaza edildi.Bulgular: Kalp ve akciğer dokularında analiz edilen beş biyokimyasal parametrede anlamlı farklar vardı. PTZ grubunun, Kalp ve akciğer MDA düzeyleri C ve ARS-60 grubuna göre anlamlı olarak yüksek bulundu ( p < 0.05). PTZ grubunun, Kalp ve akciğer MDA düzeyleri C ve ARS-60 grubuna göre anlamlı olarak yüksek bulundu. Aynı şekilde kalp'te AOPP düzeyleri VPA ve ARS-60 gruplarında anlamlı bir azalış sergilerken ( p < 0.05), akçiğerde ise gruplar arasında anlamlı bir fark yoktu ( p > 0.05). PTZ grubunun kalp'te CAT ve GSH düzeyleri diğer gruplara göre azalırken, akciğerde CAT düzeyi artığı, GSH düzeyininde azaldığı bulundu. kalp dokusundaki GSH-Px düzeyinde gruplar arasında anlamlı bir fark bulunmadı (p >0.05). GSH-Px düzeyi, akciğerde sadece ARS-30 grubunda analmlı artış göstermiştir (p < 0.05).Sonuç: Sonuç olarak, PTZ işlem görmüş farelerde oluşan nöbertlerin periferal dokularda oksidatif strese neden olur. ARS tedavisi antioksidan savunma sistemine katkı sağlayabilir., Aim: The current study investigated the effects of artemisinin on the heart and lung tissue against pentylenetetrazol-induced seizures in mice. For this purpose, malondialdehyde (MDA), advanced oxidation protein products (AOPP), Catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels were evaluated in both tissue homogenates.Material and Method: Swiss albino male mice (n=42) were used in the experiment. Animals were divided into six groups; Control (C), pentylenetetrazol (PTZ), valproate 100 mg/kg (VPA), artemisinin 30 mg/kg (ARS)+PTZ, ARS 60 mg/kg+PTZ, ARS 120 mg/kg+PTZ. On the 26th day of the experiment, the mice were sacrificed and the samples were kept at -80 0C for biochemical analysis.Results: There were significant differences in the five biochemical parameters analyzed in heart and lung tissues. Heart and lung MDA levels of the PTZ group were found to be significantly higher than the C and ARS-60 groups (p0.05). Heart CAT and GSH levels were decreased in the PTZ group compared to the other groups. However, in terms of Lung CAT levels, the PTZ group had the highest value compared to the other groups, while it had the lowest value in terms of GSH level. The GSH-Px level did not differ significantly between the groups in heart tissue (p>0.05). The lung GSH-Px level was significantly increased in the ARS-30 group when compared to the PTZ group (p

Bicyclic structures were synthesized by LACASA-DA cycloaddition reaction using (S)-BINOL as a chiral inductor. The N-2 pyridazine position was protected; the hydroxyl group was carbonylated to form the new bicyclic structure. The protective group was removed, the double bond dehydroxylated leading to the target compound. Removing of the protective group was performed using newly found ecofriendly catalyst for N-Boc deprotection. The obtained features from the model against AChE enzyme suggest that a lower the size of the ring, number of –NH-NH- groups, number of secondary aromatic amines, number of aromatic ketone groups may contribute to the inhibitory activity. The features obtained from the model against BChE enzyme suggest that the sum of topological distances between two nitrogen atoms, number of secondary aromatic amides, may be more favorable for inhibition. The features obtained from selectivity-based model suggest that the number of aromatic ethers, unsaturation content related to their molecular size and molecular shape may be more specific for the inhibition of the AChE enzyme in comparison to the BChE enzyme. Similarly, these aromatic structures inhibited the α-glucosidase enzyme at the micromolar level due to its structural feature. Inhibition values were found to be statistically significant, especially for cholinesterase enzymes. Molecular docking method was used to compare the biological activities of molecules against enzymes. The enzymes used in this study are α-glycosidase, butyrylcholinesterase, acetylcholinesterase, respectively. ADME/T analysis was performed to use some bicyclic molecules as drugs in the future.