1. Identification of unsuspected PNH-type cells in flow cytometric immunophenotypic analysis of peripheral blood and bone marrow.
- Author
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Thomason RW, Papiez J, Lee RV, and Szczarkowski W
- Subjects
- Adolescent, Adult, Aged, Bone Marrow Diseases pathology, CD55 Antigens metabolism, CD59 Antigens metabolism, Diagnosis, Differential, Flow Cytometry, Granulocytes metabolism, Humans, Immunophenotyping, Lipopolysaccharide Receptors biosynthesis, Middle Aged, Monocytes metabolism, Receptors, IgG biosynthesis, Biomarkers analysis, Bone Marrow pathology, Hemoglobinuria, Paroxysmal blood, Hemoglobinuria, Paroxysmal metabolism
- Abstract
In this report, the flow cytometric expression patterns for CD14 on monocytic cells and CD16 on granulocytic cells in peripheral blood or bone marrow specimens are illustratedfor 15 patients proven to have a paroxysmal nocturnal hemoglobinuria (PNH) phenotype by flow cytometric analysis for CD55 and CD59. The varied clinical manifestations of PNH and its rarity may make it difficult to recognize clinically. As a result, blood or bone marrow samples may be submitted for flow cytometric analysis to exclude bone marrow neoplasia or dysplasia in patients with cytopenias rather than to exclude PNH. This was true in 5 of 15 study cases. Unlike CD55 and CD59, CD14 and/or CD16 are assessed routinely in the flow cytometric analysis of blood and bone marrow samples. Recognition of abnormal patterns of CD14 and CD16 expression might permit the identification of clinically unsuspected PNH by routine flow cytometric analysis.
- Published
- 2004
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