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Glass wool is an insulation material used in large quantities; despite its popularity, it is still surprising that around 2.5 million tons of mineral wool waste is generated every year in Europe. Waste management faces numerous challenges because, with current technologies, waste cannot be cleaned and melted again for repurposing, so even the recollected glass wool ends up in landfill. Herein, we present a hydrolysis technology that decomposes the binder applied to glass fibers using exclusively water. We succeeded in decomposing the resin from the surfaces of the end-of-life fibers, originating from different sources like industrial furnace insulation and two demolition-sourced building insulation wastes. The effects of temperature, pressure, the mass flow of the water, and the applied fluid ratio were investigated. The most important parameters are the temperature and the time for the decomposition, but to minimize glass loss through the solubility of glass, the fluid ratio plays an important role as well. The fibers were efficiently cleaned after only 20 min at 300 °C by the optimized parameters, with a mass recovery of 70 wt.%. [ABSTRACT FROM AUTHOR]

Introduction: A dramatic increase of infections induced by carbapenemase-producing Enterobacterales (CPE) has been registered worldwide. The aim of this study was to evaluate the molecular epidemiology and the clinical impact of CPE strains isolated from adult inpatients.

Carbapenemase-producing Enterobacterales (CPE) have emerged and spread in Romania since 2010. According to the reports of the EuSPACE (European survey of carbapenemase-producing Enterobacteriaceae) the epidemio-logical stage of the CPE expansion in Romania has shifted from sporadic occurrence in 2013 directly to inter-regional spread in 2014-2015. In this study we aimed to provide data from the timeframe when the dissemination of the carbapenemase genes in Romania began, by retrospectively analyzing CPE strains in a tertiary care university hospital. During the period of November 2012 – October 2013 we found 107 CPE (8.78%) out of 1219 non-duplicate Enterobacterales strains. 26 isolates of various Enterobacterales species carried blaNDM-1, 83 Klebsiella pneumoniae strains were positive for blaOXA-48-like and 2 of these co-harboured blaNDM-1. The increased incidence of OXA-48 producing K. pneumoniae was linked to a two-peaked hospital outbreak during February and May 2013. The percentage of 24.3% of NDM-1 producers was alarming due to the diversity of involved species and the higher resistance levels to carbapenems compared with blaOXA-48-like gene carriers. Plasmid replicon typing revealed a great diversity of plasmids in NDM-1-positive strains, belonging to incompatibility groups A/C, FII, FIIk, HI2, L and M. The strong connection between certain plasmid groups and host species suggests that the transfer of broad host-range plasmids through conjugation does not play the main role in the successful spread of blaNDM-1 among Enterobacterales species.

Introduction: Infections due to carbapenemase-producing carbapenem-resistant Enterobacteriaceae (CPCRE) are an emerging global public health threat. The purpose of this study was to investigate phenotypic and genotypic features of CP-CRE strains isolated from hospitalized patients. Material and methods: Between 1st of January - 1st of July 2017, in the Department of Microbiology, “Dr. Constantin Opriş” County Emergency Hospital Baia Mare, Romania, 1110 strains of Enterobacteriaceae were isolated from bronchial secretions, urine, wounds and blood cultures. Bacterial identification and antimicrobial susceptibility tests were performed by conventional methods, Vitek 2 Compact and M.I.C.E. strips. We analysed all Enterobacteriaceae strains non-susceptible to carbapenems according to CLSI 2017 criteria. The modified Hodge test (MHT), the modified carbapenem inactivation method (mCIM) and the combination disks test (KPC, MBL, OXA-48 Confirm kit, Rosco Diagnostica) were used for phenotypic confirmation, whereas a multiplex PCR assay for genes blaKPC, blaNDM and blaOXA-48 was used for genetic confirmation. Results: 19 non-duplicate strains isolated from 16 patients were phenotypically identified as CP-CRE: Klebsiella pneumoniae (n=14), Escherichia coli (n=2), Providencia stuartii (n=2) and Serratia marcescens (n=1). Most strains were isolated from bronchial secretions (n=9). The carbapenem-hydrolizing enzymes were identified by the combination disks test as: KPC (n=9), OXA-48-like (n=5) and MBL (n=5). Molecular confirmation was performed in 18 phenotypically positive isolates with 100% concordant results with mCIM and combination disks test. Discrepant results were noticed with the MHT in case of 4 NDM-producers confirmed by PCR. All CP-CRE strains were resistant to all tested cephems. Three out of 9 K. pneumoniae strains tested against colistin were found resistant. Conclusions: The most common carbapenemase detected was KPC. Therapeutic options were limited in all positive cases. Rapid and reliable detection of CP-CRE is critical for preventing the spread of these pathogens

Relevanța clinică a tulpinilor Staphylococcus aureus cu susceptibilitate heterointermediară la vancomicină (hVISA) nu este complet elucidată, totuși acestea pot fi responsabile pentru eșec terapeutic la pacienții tratați cu vancomicină. Lipsa standardizării și complexitatea metodelor de detectare sunt provocări importante in identificarea acestor tulpini. Scopul studiului a fost evaluarea frecvenței tulpinilor hVISA în Spitalul Clinic Județean de Urgență Târgu-Mureș. O sută douăzeci și două de tulpini de S. aureus meticilino-rezistente (MRSA), susceptibile la vancomicina cu E-test standard (CMI≤2 mg/L), au fost testate pentru heterorezistență cu metoda E-test GRD (Glycopeptide Resistance Detection). Pentru confirmare s-a folosit analiza populațională. Douăzeci și patru de tulpini (19.5%) au fost selectate cu metoda screening. Două dintre acestea (1.63%) au fost confirmate fiind hVISA, nici un izolat nu avea susceptibilitate intermediară la vancomicină (VISA). Rata tulpinilor hVISA a fost scazută. Monitorizarea lor poate fi totuși importanta, luând in considerare ca glicopeptidele sunt larg utilizate pentru tratamentul infecțiilor severe cauzate de MRSA.

The first semi-continuous Pressurized Liquid Extraction (PLE) of hemp threshing residue with ethanol was carried out according to a 32 full factorial experimental design with pressure and temperature as independent variables at 8-10-12 MPa and 323-333-343 K, respectively. The total- and cannabidiol (CBD) yield curves were fitted to the modified two-parameter Brunner equation. Best results, concerning CBD, can be achieved at 12 MPa and 343 K. Solvent mass-consumption and operation time were considerably decreased compared to a previous supercritical fluid extraction study on the same material. Furthermore, the concentration profiles were evaluated to study the mass transfer. The winterized dry extracts were further studied in a methanol-hexane-water ternary system concerning CBD distribution ratio, showing high methanol dependency. [ABSTRACT FROM AUTHOR]