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Your search keyword '"Syutsubo, Kazuaki"' showing total 262 results
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262 results on '"Syutsubo, Kazuaki"'

15. Escherichia coli removal in down-flow hanging sponge reactors: insights from laboratory reactor studies.

Author

Tomioka, Noriko, Tran P., Thao, Aoki, Masataka, Takemura, Yasuyuki, and Syutsubo, Kazuaki

Subjects
ESCHERICHIA coli, SEWAGE purification, SPONGE (Material), PATHOGENIC bacteria, NUTRITIONAL status
Abstract

Down-flow hanging sponge (DHS) reactors, employed in domestic wastewater treatment, have demonstrated efficacy in eliminating Escherichia coli and other potentially pathogenic bacteria. The aim of this study was to elucidate the mechanism of removal of E. coli by employing a cube-shaped polyurethane sponge carrier within a compact hanging reactor. An E. coli removal experiment was conducted on this prepared sponge. Escherichia. coli level was found to decrease by more than 2 logs after passing through five nutrient-restricted DHS sponges. Conversely, a newly introduced sponge did not exhibit a comparable reduction in E. coli level. Furthermore, under conditions of optimal nutritional status, the reduction in E. coli level was limited to 0.5 logs, underscoring the crucial role of nutrient restriction in achieving effective elimination. Analysis of the sponge-associated bacterial community revealed the presence of a type VI secretion system (T6SS), a competitive mechanism observed in bacteria. This finding suggests that T6SS might play a pivotal role in contributing to the observed decline in E. coli level. [ABSTRACT FROM AUTHOR]

Published
2024
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39. Suppression of phosphorus release from eutrophic lake sediments by sediment microbial fuel cells.

Author

Takemura, Yasuyuki, Syutsubo, Kazuaki, and Kubota, Keiichi

Subjects
MICROBIAL fuel cells, PHOSPHORUS cycle (Biogeochemistry), CARBONACEOUS aerosols, SEDIMENTS, PORE water, PHOSPHORUS, SWITCHING circuits, LAKE sediments
Abstract

Sediment microbial fuel cells (SMFCs) have served as an alternative technique to suppress phosphorus release from lake sediments to water bodies and thus mitigate eutrophication. However, the phosphorus regulation mechanism remains unclear. The purpose of this research was to understand the electrochemical influence of an SMFC on the phosphorus concentration in interstitial water. In this study, a lab-scale SMFC was applied to acetate-spiked sediments (ace+) and unspiked sediments (sed) with closed-circuit (CC)/open-circuit (OC) columns, and the circuitry was switched to investigate the relationship between electron transfer and phosphorus concentration. The dissolved total phosphorus (DTP) concentration in the sediment interstitial water in CC columns significantly decreased to below 0.1 mg/L, whereas the DTP in OC columns remained high for nine weeks. After switching the circuit, the DTP in OC→CC columns dropped but that in CC→OC columns increased within one week. At the end of the experimental period, the DTP concentrations in CC/sed, CC/ace+, OC/sed, and OC/ace+ columns were 0.10 ± 0.02, 0.03 ± 0.00, 0.82 ± 0.01, and 1.66 ± 0.12 mg/L, respectively. The respective estimated anode capacitances of those columns were 2.05 ± 0.49, 5.15 ± 0.14, 0.72 ± 0.19, and 0.71 ± 0.12 nF. We concluded that the phosphorus may have been electrochemically attracted and retained on the anode in the sediment because the adsorbed DTP contents and the increased anode capacitances were strongly correlated. Thus, SMFCs can be used for suppressing phosphorus release from eutrophic lake sediments. [ABSTRACT FROM AUTHOR]

Published
2022
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44. In situ accessibility of Escherichia coli 23S rRNA to fluorescently labeled oligonucleotide probes

Author

Fuchs, Bernhard M., Syutsubo, Kazuaki, Ludwig, Wolfgang, and Amann, Rudolf

Subjects
In situ hybridization -- Research, Nucleic acid probes -- Research, Escherichia coli -- Research, Biological sciences
Abstract

Researchers have constructed an accessibility map for the 23S rRNA of E. coli that can be used to develop oligonucleotide probes for fluorescence in situ hybridization. Accessibility to probes varied substantially, with the best signal generated from a probe that was complementary to positions 2018 to 2035.

Published
2001

45. Performance evaluation of a down-flow hanging sponge (DHS) reactor as a decentralized domestic wastewater treatment system in tropical regions

Author

Danshita, Tsuyoshi, primary, Yoochatchaval, Wilasinee, additional, Takemura, Yasuyuki, additional, Miyaoka, Yuma, additional, Kada, Mai, additional, Tepjun, Wachira, additional, Thonglee, Sirikes, additional, Sonaka, Hideaki, additional, Yamaguchi, Takashi, additional, Tomioka, Noriko, additional, Banjongproo, Pathan, additional, Okadera, Tomohiro, additional, Ebie, Yoshitaka, additional, and Syutsubo, Kazuaki, additional

Published
2020
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49. Molecular characterization of bacterial populations in petroleum-contaminated groundwater discharged from underground crude oil storage cavities

Author

Watanabe, Kazuya, Watanabe, Kanako, Kodama, Yumiko, Syutsubo, Kazuaki, and Harayana, Shigeaki

Subjects
Water -- Bacteriology, Petroleum -- Storage, Biological sciences
Abstract

Petroleum-contaminated groundwater from crude oil storage cavities contains many bacteria that do not occur in normal groundwater. Many belong to the Proteobacteria, according to researchers who used PCR to identify 28 different 16S ribosomal DNA sequences.

Published
2000

50. Phylogenetic diversity of mesophilic and thermophilic granular sludges dtermined by 16S rRNA gene analysis

Author

Sekigchi, Yuji, Kamagata, Yoichi, Syutsubo, Kazuaki, Ohashi, Akiyoshi, Harada, Hideki, and Nakamura, Kazunori

Subjects
Sludge -- Research, Anaerobic bacteria -- Research, Bacteria, Thermophilic -- Research, Biological sciences
Abstract

Two types of methanogenic granular sludge mesophilic and thermophilic, were compared for microbial diversity. PCR was used to construct 16S rDNA clone libraries. There was affiliation with the domain Archaea and the domain Bacteria. The 16S rDNA clones within the domain Archaea showed fairly close links with those of methanogens. The delta subclass of the Proteobacteria was a major group represented in the mesophilic clone library, with the Bacteria.

Published
1998

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