28 results on '"Syahzuwan Hassan"'
Search Results
2. Characterization of New Alpha Zero (α0) Thalassaemia Deletion (−−GB) among Malays in Malaysian Population
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Norafiza Mohd Yasin, Faidatul Syazlin Abdul Hamid, Syahzuwan Hassan, Yuslina Mat Yusoff, Ermi Neiza Mohd Sahid, and Ezalia Esa
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GB deletion ,alpha zero thalassaemia ,deletional characterization ,unique mutation among Malays ,Medicine (General) ,R5-920 - Abstract
Malaysia is a multicultural and multiethnic country comprising numerous ethnic groups. From the total population of 32.7 million, Malays form the bulk of the Bumiputera in Malaysia comprise about 69.9%, followed by Chinese 22.8%, Indian 6.6%, and others 0.7%. The heterogeneous population and increasing numbers of non-citizens in this country affects the heterogeneity of genetic diseases, diversity, and heterogeneity of thalassaemia mutations. Alpha (α)-thalassaemia is an inherited haemoglobin disorder characterized by hypochromic microcytic anaemia caused by a quantitative reduction in the α-globin chain. A majority of the α-thalassaemia are caused by deletions in the α-globin gene cluster. Among Malays, the most common deletional alpha thalassaemia is −α3.7 deletion followed by −−SEA deletion. We described the molecular characterization of a new −−GB deletion in our population, involving both alpha genes in cis. Interestingly, we found that this mutation is unique among Malay ethnicities. It is important to diagnose this deletion because of the 25% risk of Hb Bart’s with hydrops fetalis in the offspring when in combination with another α0- thalassaemia allele. MLPA is a suitable method to detect unknown and uncommon deletions and to characterize those cases which remain unresolved after a standard diagnostic approach.
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- 2023
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3. Molecular and hematological studies in a cohort of beta zero South East Asia deletion (β°-thal SEA) from Malaysian perspective
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Norafiza Mohd Yasin, Faidatul Syazlin Abdul Hamid, Syahzuwan Hassan, Aziee Sudin, Haiyuni Yassim, Ermi Neiza Mohd Sahid, Yuslina Mat Yusoff, Ezalia Esa, and Mohamed Saleem
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beta-thalassaemia ,HPFH ,deltabeta thalassaemia ,deletional mutation ,β°-thal SEA ,β° deletion ,Pediatrics ,RJ1-570 - Abstract
AbstractWe report the haematological parameters and molecular characterization of beta zero (β°) South East Asia (SEA) deletion in the HBB gene cluster with unusually high levels of Hb F compared to a classical heterozygous beta zero (β°)-thalassaemia.MethodsRetrospective study on 17 cases of (β°) South East Asia (SEA) deletion from 2016 to 2019 referred to Institute for Medical Research were conducted. The clinical information and haematological profiles were evaluated. The mutation was analyzed, and the results were compared with other β°-thalassaemia groups. For HBB gene genotyping, all the cases were subjected for multiplex gap-PCR, 5 cases were subjected for HBB gene sequencing for exclusion of compound heterozygous with other beta variants. Co-inheritance of α-thalassaemia were determined using multiplex gap-PCR and multiplex ARMS-PCR.ResultsSeventeen cases were positive for β°-thal SEA deletion. Fifteen cases were heterozygous and two were compound heterozygous for β°-thal SEA deletion. The results were compared with 182 cases of various heterozygous β° deletions and mutations. The mean Hb for heterozygous β°-thal SEA deletion (13.44 ± 1.45 g/dl) was normal and significantly higher than heterozygous IVS 1-1 and Codon 41/42 (post hoc test, p
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- 2022
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4. Gene Mutation Spectrum among Alpha-Thalassaemia Patients in Northeast Peninsular Malaysia
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Divashini Vijian, Wan Suriana Wan Ab Rahman, Kannan Thirumulu Ponnuraj, Zefarina Zulkafli, Rosnah Bahar, Norafiza Yasin, Syahzuwan Hassan, and Ezalia Esa
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α-thalassaemia ,genotype ,mutation ,globin ,prevalence ,molecular analysis ,Medicine (General) ,R5-920 - Abstract
(1) Background: Alpha (α)-thalassaemia is a genetic disorder that affects 5% of the world population. Deletional or nondeletional mutations of one or both HBA1 and HBA2 on chromosome 16 will result in reduced production of α-globin chains, a component of haemoglobin (Hb) that is required for the formation of red blood cells (RBCs). This study aimed to determine the prevalence, haematological and molecular characterisations of α-thalassaemia. (2) Method: The parameters were based on full blood count, high-performance liquid chromatography and capillary electrophoresis. The molecular analysis involved gap-polymerase chain reaction (PCR), multiplex amplification refractory mutation system-PCR, multiplex ligation-dependent probe amplification and Sanger sequencing. (3) Results: With a total cohort of 131 patients, the prevalence of α-thalassaemia was 48.9%, leaving the remaining 51.1% with potentially undetected α gene mutations. The following genotypes were detected: -α3.7/αα (15.4%), -α4.2/αα (3.7%), --SEA/αα (7.4%), αCSα/αα (10.3%), αAdanaα/αα (0.7%), αQuong Szeα/αα (1.5%), -α3.7/-α3.7 (0.7%), αCSα/αCSα (0.7%), -α4.2/αCSα (0.7%), –SEA/αCSα (1.5%), –SEA/αQuong Szeα (0.7%), -α3.7/αAdanaα (0.7%), --SEA/-α3.7 (2.2%) and αCSα/αAdanaα (0.7%). Indicators such as Hb (p = 0.022), mean corpuscular volume (p = 0.009), mean corpuscular haemoglobin (p = 0.017), RBC (p = 0.038) and haematocrit (p = 0.058) showed significant changes among patients with deletional mutations, but not between patients with nondeletional mutations. (4) Conclusions: A wide range of haematological parameters was observed among patients, including those with the same genotype. Thus, a combination of molecular technologies and haematological parameters is necessary for the accurate detection of α-globin chain mutations.
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- 2023
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5. Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia
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Syahzuwan Hassan, Rosnah Bahar, Muhammad Farid Johan, Ezzeddin Kamil Mohamed Hashim, Wan Zaidah Abdullah, Ezalia Esa, Faidatul Syazlin Abdul Hamid, and Zefarina Zulkafli
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PCR ,thalassemia ,sequencing ,NGS ,TGS ,CNV ,Medicine (General) ,R5-920 - Abstract
Thalassemia is one of the most heterogeneous diseases, with more than a thousand mutation types recorded worldwide. Molecular diagnosis of thalassemia by conventional PCR-based DNA analysis is time- and resource-consuming owing to the phenotype variability, disease complexity, and molecular diagnostic test limitations. Moreover, genetic counseling must be backed-up by an extensive diagnosis of the thalassemia-causing phenotype and the possible genetic modifiers. Data coming from advanced molecular techniques such as targeted sequencing by next-generation sequencing (NGS) and third-generation sequencing (TGS) are more appropriate and valuable for DNA analysis of thalassemia. While NGS is superior at variant calling to TGS thanks to its lower error rates, the longer reads nature of the TGS permits haplotype-phasing that is superior for variant discovery on the homologous genes and CNV calling. The emergence of many cutting-edge machine learning-based bioinformatics tools has improved the accuracy of variant and CNV calling. Constant improvement of these sequencing and bioinformatics will enable precise thalassemia detections, especially for the CNV and the homologous HBA and HBG genes. In conclusion, laboratory transiting from conventional DNA analysis to NGS or TGS and following the guidelines towards a single assay will contribute to a better diagnostics approach of thalassemia.
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- 2023
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6. Distribution of Alpha Thalassaemia Gene Variants in Diverse Ethnic Populations in Malaysia: Data from the Institute for Medical Research
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Syahzuwan Hassan, Nurul Mohamed, Punithawathy Yelumalai, Mohamed Saleem, Nisha Sabrina Aloysious, and Rahimah Ahmad
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alpha thalassaemia ,Hb Adana ,Malaysia ,Sabah ,Sarawak ,Orang Asli ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Alpha thalassaemia is highly prevalent in the plural society of Malaysia and is a public health problem. Haematological and molecular data from 5016 unrelated patients referred from various hospitals to the Institute for Medical Research for α thalassaemia screening from 2007 to 2010 were retrieved. The aims of this retrospective analysis were to describe the distribution of various alpha thalassaemia alleles in different ethnic groups, along with their genotypic interactions, and to illustrate the haematological changes associated with each phenotype. Amongst the patients, 51.2% (n = 2567) were diagnosed with α thalassaemia. Of the 13 α thalassaemia determinants screened, eight different deletions and mutations were demonstrated: three double gene deletions, ––SEA, ––THAI, ––FIL; two single-gene deletions, α–3.7 and –α4.2; and three non-deletion mutations, Cd59G > A (haemoglobin [Hb] Adana), Cd125T > C (Hb Quong Sze) and Cd142 (Hb Constant Spring). A high incidence of α–3.7 deletion was observed in Malays, Indians, Sabahans, Sarawakians and Orang Asli people. However, the ––SEA deletion was the most common cause of alpha thalassaemia in Chinese, followed by the α–3.7 deletion. As many as 27 genotypic interactions showed 1023 α thalassaemia silent carriers, 196 homozygous α+ thalassaemia traits, 973 heterozygous α0 thalassaemia carriers and 375 patients with Hb H disease. Statistical analysis showed a significant difference in the distribution of α thalassaemia determinants amongst the various ethnic groups. Hence, the heterogeneous distribution of common determinants indicated that the introduction of an ethnicity-targeted hierarchical α thalassaemia screening approach in this multi-ethnic Malaysian population would be effective.
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- 2013
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7. ß-globin Gene Mutations Among ß-thalassemia and ß-variant patients in a teaching Hospital in Universiti Sains Malaysia, Kubang Kerian, Kelantan
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Syahzuwan Hassan
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General Medicine - Published
- 2022
8. Prevalence of ticks (Acari: Ixodidae) and Theileria annulata infection of cattle in Gezira State, Sudan
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Syahzuwan Hassan, Diaeldin A. Salih, S.K. Ahmed, and M.A. Hayati
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0301 basic medicine ,Veterinary medicine ,Coat ,Epidemiology ,030231 tropical medicine ,IFA test ,Tropical theileriosis ,lcsh:Infectious and parasitic diseases ,Sudan ,03 medical and health sciences ,0302 clinical medicine ,Ticks ,Theileria ,parasitic diseases ,Acari ,lcsh:RC109-216 ,Original Research article ,biology ,030108 mycology & parasitology ,biology.organism_classification ,Zebu ,Theileria annulata ,Infectious Diseases ,Parasitology ,Ixodidae ,Gezira State ,Hard ticks - Abstract
This study was conducted in Gezira State, central Sudan during January, May and August 2014 to determine species of ticks infesting cattle and prevalence of Theileria annulata infection and antibodies. A total of 200 head of cattle were sampled for ticks, blood smears, lymph node biopsy smears and sera from five study areas. Zebu (Butana and Kenana) and cross-bred (Zebu X Friesian) cattle of both sexes were sampled. Cattle age groups were younger than one year, one year to younger than four years and four years and older. Coat colour of cattle was recorded. The results revealed that cattle were infested with Rhipicephalus evertsi evertsi (n = 562, 51.6%), Hyalomma anatolicum (n = 289, 26.5%), H. rufipes (n = 70, 6.4%), R. sanguineus sanguineus (n = 59, 5.4%), R. decoloratus (n = 39, 3.6%), Amblyomma lepidum (n = 35, 3.2%), H. impeltatum (n = 26, 2.4%) and H. dromedarii (n = 9, 0.8%). Out of 200 blood and biopsy smears, 33 (16.5%) showed piroplasms for Theileria spp., while 29 (14.5%) were positive for Theileria spp. schizonts. Seropositivity for T. annulata using indirect fluorescent antibody (IFA) test was 94 (47%). It is concluded that there are three genera of hard ticks and eight species in Gezira State and tropical theileriosis is endemic in the state. These findings are the first records in Gezira State, Central Sudan and it is highly recommended to consider the endemicity of tropical theileriosis particularly in cattle upgrading programmes.
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- 2020
9. Coinheritance of δ-Thalassemia and a Heterozygous XmnI Polymorphism Mask the Diagnosis of Borderline Hb A2 β-Thalassemia: A Diagnostic Challenge
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Syahzuwan Hassan, Zubaidah Zakaria, Norafiza Mohd Yasin, Yuslina Mat Yusoff, Faidatul Syazlin Abdul Hamid, and Ezalia Esa
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Genetics ,business.industry ,hemic and lymphatic diseases ,Thalassemia ,Biochemistry (medical) ,Clinical Biochemistry ,Xmni polymorphism ,Medicine ,Hematology ,business ,medicine.disease ,Genetics (clinical) - Abstract
Detection of β-thalassemia (β-thal) carriers depends significantly on an increase in Hb A2 levels. Mutations on the δ-globin gene are not pathogenically relevant, but it reduces the Hb A2 and masks...
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- 2019
10. An Insight on Beta Zero (β0) Southeast Asian Deletion (βSEA); Is it a β0-Thalassemia or Hereditary Persistence of Fetal Hemoglobin (HPFH)
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Faidatul Syazlin Abdul Hamid, Haiyuni Mohd Yassim, Norafiza Mohd Yasin, Aziee Sudin, Syahzuwan Hassan, and Ezalia Esa
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Genetics ,Hereditary persistence of fetal hemoglobin ,Thalassemia ,Biochemistry (medical) ,Clinical Biochemistry ,medicine ,Hematology ,Biology ,medicine.disease ,Southeast asian ,Beta (finance) ,Genetics (clinical) - Abstract
We report hematological parameters and molecular characterization of the β0 Southeast Asian (βSEA) deletion with unusually high levels of Hb F as compared to classical β-thalassemia (β-thal) trait....
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- 2019
11. Identification of a Novel α-Thalassemia Deletion: –(α)4.9 from a Current Multiplex Gap-Polymerase Chain Reaction
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Faidatul Syazlin Abdul Hamid, Ermi Neiza Mohd Sahid, Syahira Lazira Omar, Yuslina Mat Yusoff, Zubaidah Zakaria, Velintina Matnih, Mohd Bakri Bidin, Norafiza Mohd Yasin, Ezalia Esa, and Syahzuwan Hassan
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Genetics ,Thalassemia ,Biochemistry (medical) ,Clinical Biochemistry ,Genetic disorder ,Hematology ,Biology ,medicine.disease ,law.invention ,Chain (algebraic topology) ,law ,hemic and lymphatic diseases ,medicine ,Identification (biology) ,Multiplex ,Gene ,Genetics (clinical) ,Polymerase chain reaction - Abstract
α-Thalassemia (α-thal) is a genetic disorder caused by a reduced production of α-globin chain and commonly results from a large deletion involving the α-globin gene. This study aimed to investigate...
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- 2019
12. Application of Molecular Diagnostics in Detection of β-Thalassemia and Hemoglobin Variants at Hospital Canselor Tuanku Muhriz UKM, Kuala Lumpur, Malaysia
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Hamidah Alias, Ainoon Othman, Syahzuwan Hassan, Azlin Ithnin, Jalil Norunaluwar, Hafiza Alauddin, Malisa Mohd Yusoff, Zarina Abdul Latiff, Azma Rz, and Nor Rafeah Tumian
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medicine.medical_specialty ,Kuala lumpur ,business.industry ,Thalassemia ,Biochemistry (medical) ,Clinical Biochemistry ,Hemoglobin variants ,Hematology ,Molecular diagnostics ,medicine.disease ,Internal medicine ,medicine ,business ,Genetics (clinical) - Published
- 2019
13. Discovery of New Deletional α-Thalassemia Mutations Detected by Multiplex Ligation-Dependent Probe Amplification
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Norafiza Mohd Yasin, Zubaidah Zakaria, Ezalia Esa, Yuslina Mat Yusoff, Ermi Neiza Mohd Sahid, Faidatul Syazlin Abdul Hamid, and Syahzuwan Hassan
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Population migration ,Thalassemia ,Biochemistry (medical) ,Clinical Biochemistry ,medicine ,Multiplex ,Hematology ,Multiplex ligation-dependent probe amplification ,Biology ,medicine.disease ,Molecular biology ,Genetics (clinical) - Abstract
Malaysia is a multi-racial country with increasing population migration. Multiplex ligation-dependent probe amplification (MLPA) is useful for detection of known and unknown mutations in the α- and...
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- 2019
14. β-Thalassemia Intermedia Due to Coinheritance of Three Unique α-Globin Cluster Duplications
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Hishamsah Ibrahim, Ezalia Esa, Syahzuwan Hassan, Nor Rizan Kamalludin, Zubaidah Zakaria, Yuslina Mat Yusoff, Nurul Zawani Zaini, and Norafiza Mohd Yasin
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Genetics ,β thalassemia intermedia ,Biochemistry (medical) ,Clinical Biochemistry ,Hematology ,Biology ,Disease cluster ,Genetics (clinical) ,α globin - Published
- 2019
15. Molecular, serological and parasitological survey of Theileria annulata in North Kordofan State, Sudan
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Diaeldin A. Salih, Syahzuwan Hassan, G.M. Mohammed-Ahmed, and A.M. El Hussein
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0301 basic medicine ,Male ,Veterinary medicine ,Veterinary parasitology ,Ixodidae ,Prevalence ,Antibodies, Protozoan ,Cattle Diseases ,Tick ,Polymerase Chain Reaction ,Hyalomma dromedarii ,Tropical theileriosis ,Serology ,Sudan ,03 medical and health sciences ,Ticks ,Surveys and Questionnaires ,parasitic diseases ,Theileria ,Animals ,General Veterinary ,biology ,030108 mycology & parasitology ,DNA, Protozoan ,biology.organism_classification ,Zebu ,Theileria annulata ,Theileriasis ,Tick Infestations ,Parasitology ,Cattle ,Female - Abstract
This survey was conducted to detect Theileria annulata infection in indigenous (Zebu) and cross (indigenous × Friesian) cattle (n 604) of different sex & age groups, and to investigate prevalence of ticks infesting cattle in North Kordofan State, Sudan. Ticks, blood smears, serum samples and blood spots on filter paper were collected from four municipalities at four monthly intervals during the period from August 2008 to July 2009. Out of 604 blood smears, 69 (11.4%, 95% CI: 16.9–5.9) were positive for Theileria piroplasms; whereas, out of 240 serum samples, 164 (68.3%, 95% CI: 89.3–47.3) were positive for antibodies against T. annulata using IFA test. Using PCR, out of 100 blood spots on filter paper, 8 (8%, 95% CI: 9.6–6.4) revealed T. annulata DNA. The highest prevalence of Theileria spp. piroplasms and T. annulata antibodies was recorded in August (15.9 ± 4.6) followed by February (13.3 ± 3.8) and the lowest was in May (3.9 ± 1.0). Among cattle breeds, the highest prevalence of piroplasms (11.9 ± 5.0) and T. annulata antibodies (68.9 ± 10) was among zebu cattle. PCR assays revealed the highest prevalence rate of T. annulata DNA in February (12.5 ± 1.4) followed by August (6.1 ± 0.7) and the lowest was in May (5.7 ± 0.7). According to cattle breeds, T. annulata DNA was detected in cross-bred cattle (3.3 ± 0.4) more than zebu cattle (10 ± 1.6). Seven tick species belonging to four genera and were identified, Amblyomma lepidum, Hyalomma dromedarii, H. impeltatum, H. rufipes, Rhipicephalus evertsi evertsi, Rhipicephalus decoloratus, R. annulatus. The most abundant tick species was H. rufipes followed by H. impeltatum, R. e. evertsi, H. dromedarii, R. decoloratus, A. lepidum and R. annulatus. Theileria annulata has been detected for the first time in North Kordofan State, though at a low prevalence rate. This finding is an alarming situation since tropical theileriosis is an emerging disease and it is widely believed that this economically important tick-borne parasite does not exist in this state. The possible alternative vector(s) of T. annulata in the absence of the known vector H. anatolicum is discussed.
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- 2017
16. Assessment of the prevalence of Theileria lestoquardi in sheep from the Sudan using serological and molecular methods
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Syahzuwan Hassan, Roger Pelle, Ulrike Seitzer, David Odongo, A.M. El Hussein, Shawgi Mohamed Hassan, Richard P. Bishop, J. S. Ahmed, R.A. Skilton, and Mohammed A. Bakheit
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Male ,Veterinary medicine ,040301 veterinary sciences ,Concordance ,030231 tropical medicine ,Loop-mediated isothermal amplification ,Sheep Diseases ,Enzyme-Linked Immunosorbent Assay ,Polymerase Chain Reaction ,Asymptomatic ,Serology ,law.invention ,Sudan ,0403 veterinary science ,03 medical and health sciences ,0302 clinical medicine ,Food Animals ,law ,Theileria ,parasitic diseases ,Prevalence ,medicine ,Animals ,Polymerase chain reaction ,Tick-borne disease ,Sheep ,Geography ,biology ,04 agricultural and veterinary sciences ,Gold standard (test) ,medicine.disease ,biology.organism_classification ,Theileriasis ,Female ,Animal Science and Zoology ,medicine.symptom - Abstract
Malignant theileriosis of sheep and goats caused by Theileria lestoquardi is considered to be among the most important tick borne diseases in the Sudan. Information on the prevalence of the disease in different parts of the Sudan is limited. The purpose of this study was to estimate the prevalence of the disease in five states of the Sudan using molecular and serological assays. A total of 393 blood and serum samples from clinically asymptomatic sheep were analysed using nested reverse line blot (nRLB) and loop mediated isothermal amplification (LAMP), as well as an enzyme-linked immunosorbent assay (ELISA). The results indicated a sero-prevalence of 33.8% while RLB and LAMP assays revealed molecular prevalences of 29.5 and 22.6% respectively. The prevalence of Theileria lestoquardi varied significantly according to the geographical origin of the infected animals, whereas age and gender did not have a significant effect. RLB data indicated that T. lestoquardi usually occurred as a co-infection with the non-pathogenic Theileria ovis. Using RLB as a gold standard, a sensitivity of 68.1% and a specificity of 96.4% were recorded for LAMP and a sensitivity of 75.9% and a specificity of 83.8% for ELISA. The Kappa coefficient between nRLB and LAMP indicated a significant level of agreement (0.692), but only moderate concordance (0.572) between nRLB and ELISA. The results of the present study confirm and extend earlier findings regarding the widespread of T. lestoquardi infections in sheep in the Sudan. The data provide evidence that should enable the veterinary authorities to deploy appropriate control measures.
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- 2019
17. Transfusion Dependent Homozygous α-Thalassemia in Patients Associated with Hypospadias in Three Survivors
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Faidatul Syazlin Abdul Hamid, Aini Salmah Rabiee, Mohd Hishamshah Mohd Ibrahim, Zubaidah Zakaria, Yeoh Seoh Leng, Nur Aisyah Aziz, Syahira Lazira Omar, Siti Hida Hajira Mohamad Arif, Rahimah Ahmad, and Syahzuwan Hassan
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medicine.medical_specialty ,Fetus ,Mutation ,business.industry ,Thalassemia ,Point mutation ,medicine.disease ,Bioinformatics ,medicine.disease_cause ,Gastroenterology ,Hypospadias ,hemic and lymphatic diseases ,Hydrops fetalis ,Internal medicine ,Genotype ,Medicine ,business ,Gene - Abstract
Alpha-thalassemia results from a dysfunction of the α-globin gene. Types of mutations include large deletions and point mutations. The most severe form of α-thalassemia is hydrops fetalis, which is caused by homozygosity of certain types of either deletion or point mutations, and some cases were the results of a combination of both. Here, we describe three cases of homozygous α-thalassemia who continue to survive, all with hypospadias. The first two cases were 5-year-old twins that were diagnosed with homozygous SEA deletion and the first description of a 20- month-old child with the genotype of the homozygous Cd 59 (GGC>GAC) mutation of the HBA2 gene. Prognoses for any α-thalassemia mutation types that are known to lead to hydrops fetalis in male fetuses should be informed about the potential survival associated with hypospadias.
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- 2015
18. Compound Heterozygous -30 (T>C) and Cd 41/42 (-TTCT) Leading to β-Thalassemia Major in a Malay Autistic Patient
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Rahimah Ahmad, Syahzuwan Hassan, primary
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- 2015
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19. Transfusion Dependent Homozygous α-Thalassemia in Patients Associated with Hypospadias in Three Survivors
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Rahimah Ahmad, Syahzuwan Hassan, primary
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- 2015
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20. Ticks (Acari: Ixodidae) infesting camels (Camelus dromedarius) in Northern Sudan
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Syahzuwan Hassan and A. Elghali
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endocrine system ,Veterinary medicine ,General Veterinary ,biology ,Rhipicephalus sanguineus ,General Medicine ,Tick ,biology.organism_classification ,medicine.disease_cause ,Hyalomma dromedarii ,parasitic diseases ,Infestation ,medicine ,Acari ,Hyalomma ,Nymph ,Ixodidae - Abstract
Monthly total body tick collections from 13-20 camels were conducted for 2 consecutive years (2000-2001) in Northern Sudan. Tick populations were correlated with locality, season, predeliction site, sex and coat colour. Hyalomma dromedarii was found to be the predominant (89%) tick species infesting the camels. Other tick species found in very low numbers were Hyalomma impeltatum (7.7%), Hyalomma anatolicum anatolicum (3.3%), Hyalomma truncatum (0.29%), Hyalomma marginatum rufipes (0.25%), Rhipicephalus praetextatus (0.30%) and Rhipicephalus sanguineus group (0.09%). Nymphs of the genus Hyalomma were collected in significant numbers. Adult ticks significantly preferred to attach to the lower parts of the camel's body for feeding while the nymphs preferred the back of the animal. Female camels harboured more ticks than males while higher infestations were recorded on camels with a grey coat colour compared to those with a brown coat colour. Ticks were found on camels throughout the year and increased in numbers during March to October with a peak in September.
- Published
- 2009
21. Preliminary survey of ticks (Acari: Ixodidae) on cattle in Central Equatoria State, Southern Sudan
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Diaeldin A. Salih, A.M. El Hussein, Syahzuwan Hassan, I. I. Julla, and Frans Jongejan
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Male ,Veterinary medicine ,Southern Sudan ,Rhipicephalus sanguineus ,Cattle Diseases ,Tick ,ticks ,Sudan ,Ticks ,Species Specificity ,preliminary survey ,Animals ,East Coast fever ,Acari ,Phylogeny ,lcsh:Veterinary medicine ,General Veterinary ,biology ,General Medicine ,biology.organism_classification ,Zebu ,Breed ,Theileriasis ,Tick Infestations ,lcsh:SF600-1100 ,Female ,Cattle ,Seasons ,Sentinel Surveillance ,Ixodidae ,Amblyomma variegatum - Abstract
SALIH, D.A., JULLA, I.I., HASSAN, S.M., EL HUSSEIN, A.M. & JONGEJAN, F. 2008. Preliminary survey of ticks (Acari: Ixodidae) on cattle in Central Equatoria State, Southern Sudan. Onderstepoort Journal of Veterinary Research, 75:47–53 In a preliminary survey conducted in 2005, the species composition and seasonality of ticks infesting cattle in Central Equatoria State, Southern Sudan was determined. Three locations were selected (Gumbo, Khor Rumla and Nyaing) and surveyed every 3 months. Two cattle herds in each of the three locations were visited four times during the study period. Total body collections of ticks were made from each of five cattle (Nilotic Zebu breed) kept in six different herds. Four tick genera and ten species were identified. The tick species identified were Amblyomma lepidum, Amblyomma variegatum, Boophilus annulatus, Boophilus decoloratus, Hyalomma marginatum rufipes, Hyalomma truncatum, Rhipicephalus appendiculatus, Rhipicephalus evertsi evertsi, Rhipicephalus praetextatus and Rhipicephalus sanguineus group. The highest number of ticks was collected in October during the rainy season. A finding of great significance was that R. appendiculatus, vector of East Coast fever, has now firmly established itself throughout the year with possible implications for cattle production in Central Equatoria State.
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- 2008
22. Distribution and application of ELISA for the seroprevalence of tick-borne diseases in Central Equatoria State, Sudan
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I. I. Julla, Syahzuwan Hassan, Diaeldin A. Salih, K-H. Zessin, M. N. Kyule, and A.M. El Hussein
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Veterinary medicine ,Theileria parva ,Antibodies, Protozoan ,Babesia ,Cattle Diseases ,Enzyme-Linked Immunosorbent Assay ,Sudan ,Seroepidemiologic Studies ,Theileria ,parasitic diseases ,medicine ,Seroprevalence ,East Coast fever ,Animals ,Babesia bigemina ,Tick-borne disease ,General Veterinary ,General Immunology and Microbiology ,biology ,General Medicine ,medicine.disease ,biology.organism_classification ,Antibodies, Bacterial ,Anaplasma marginale ,Tick-Borne Diseases ,Cattle ,Seasons - Abstract
This study was carried out to estimate the prevalence of Theileria parva, Theileria mutans, Babesia bigemina and Anaplasma marginale antibodies among naturally infected cattle in Central Equatoria State, Southern Sudan using indirect enzyme-linked immunosorbent assay (ELISA). Three locations (Khor Rumla, Nyaing and Gumbo) were selected and surveyed every 3 months during the period from January to December 2005. Six cattle herds in the three locations were visited four times during the study period. During each visit, 150 serum samples were collected from indigenous cattle of both sex and of different age groups. Assessment of antibodies against tick-borne diseases was carried out using indirect ELISA. The overall seroprevalence rates of T. parva, T. mutans, B. bigemina and A. marginale were found to be 58.2%, 88.9%, 51.1% and 37.8% respectively. The highest seroprevalence of T. parva was observed in Gumbo (69%). An overall seroprevalence of 43.3% for T. parva was reported in July which was significantly lower than other seasons. Older animals showed higher seroprevalence than younger ones. The implications of these results on the epidemiology of tick-borne diseases are discussed with emphasis on East Coast fever.
- Published
- 2008
23. Comparisons among two serological tests and microscopic examination for the detection of Theileria annulata in cattle in northern Sudan
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Syahzuwan Hassan, A.M. El Hussein, and Diaeldin A. Salih
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Theileriasis ,Protozoan Proteins ,Antibodies, Protozoan ,Enzyme-Linked Immunosorbent Assay ,Biology ,Virology ,Sensitivity and Specificity ,Theileria annulata ,Serology ,Sudan ,Food Animals ,parasitic diseases ,Animals ,Animal Science and Zoology ,Cattle ,Serologic Tests ,Fluorescent Antibody Technique, Indirect - Abstract
We tested the agreement between microscopic examination (ME), a surface protein-detecting enzyme-linked immunosorbent assay (TaSP ELISA) and an indirect fluorescent assay (IFA) for detection of Theileria annulata in 2,661 naturally infected cattle from northern Sudan (samples collected between June 2001 and July 2002). In the ME, we detected piroplasms in 364/2,661 cattle (14%), and the kappas between the ME and the serological tests were poor (TaSP ELISA 10%; IFA 8%). The TaSP ELISA detected 885/2,661 cattle as positive, and the Rogan-and-Gladen corrected true prevalence of this sample was estimated to be 30%. The relative sensitivity and specificity of the IFA (compared to the previously validated TaSP ELISA) were 70.7% and 81.8%, respectively.
- Published
- 2006
24. Natural infection rates and transmission of Theileria annulata by Hyalomma anatolicum anatolicum ticks in the Sudan
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Syahzuwan Hassan, A.G. Lazarus, A.M. El Hussein, Diaeldin A. Salih, and O.E. Sharieff
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Veterinary medicine ,General Veterinary ,Transmission (medicine) ,parasitic diseases ,Theileria ,Outbreak ,Hyalomma anatolicum ,General Medicine ,Biology ,Theileria annulata ,biology.organism_classification ,Nymph ,Virology - Abstract
SALIH, D.A., SHARIEFF, O.E., LAZARUS, A.G., HASSAN, S.M. & EL HUSSEIN, A.M. 2005. Natural infection rates and transmission of Theileria annulata by Hyalomma anatolicum anatolicum ticks in the Sudan. Onderstepoort Journal of Veterinary Research, 303–307 Hyalomma anatolicum anatolicum nymphs were collected from two localities in the Sudan: Eddamer in Northern Sudan and Wad-Medani in Central Sudan. They were allowed to moult to adult ticks, which were assessed for Theileria infection in their salivary glands using Feulgen stain. At Eddamer, 49.6 % of 123 ticks examined were infected with Theileria and the mean intensity of infection was 1.3 (i.e. the number of infected acini/number of infected ticks). At Wad-Medani, 8.6 % of 162 ticks were infected and the mean intensity of infection was 7.9. The prevalence of infection was higher in female than in male ticks at both localities. When adult H. a. anatolicum were applied onto two susceptible calves, both animals developed the severe form of theileriosis.
- Published
- 2005
25. Distribution of Alpha Thalassaemia Gene Variants in Diverse Ethnic Populations in Malaysia: Data from the Institute for Medical Research.
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Rahimah Ahmad, Mohamed Saleem, Aloysious, Nisha Sabrina, Yelumalai, Punithawathy, Nurul Mohamed, and Syahzuwan Hassan
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PLURAL societies ,PUBLIC health ,DELETION mutation ,PHENOTYPES ,HEMOLYTIC anemia - Abstract
Alpha thalassaemia is highly prevalent in the plural society of Malaysia and is a public health problem. Haematological and molecular data from 5016 unrelated patients referred from various hospitals to the Institute for Medical Research for α thalassaemia screening from 2007 to 2010 were retrieved. The aims of this retrospective analysis were to describe the distribution of various alpha thalassaemia alleles in different ethnic groups, along with their genotypic interactions, and to illustrate the haematological changes associated with each phenotype. Amongst the patients, 51.2% (n = 2567) were diagnosed with α thalassaemia. Of the 13 α thalassaemia determinants screened, eight different deletions and mutations were demonstrated: three double gene deletions, - -
SEA , - -THAI , --FIL ; two single-gene deletions, α-3.7 and - α4.2 ; and three non-deletion mutations, Cd59G > A (haemoglobin [Hb] Adana), Cd125T > C (Hb Quong Sze) and Cd142 (Hb Constant Spring). A high incidence of α-3.7 deletion was observed in Malays, Indians, Sabahans, Sarawakians and Orang Asli people. However, the - -SEA deletion was the most common cause of alpha thalassaemia in Chinese, followed by the α-3.7 deletion. As many as 27 genotypic interactions showed 1023 α thalassaemia silent carriers, 196 homozygous α+ thalassaemia traits, 973 heterozygous α0 thalassaemia carriers and 375 patients with Hb H disease. Statistical analysis showed a significant difference in the distribution of α thalassaemia determinants amongst the various ethnic groups. Hence, the heterogeneous distribution of common determinants indicated that the introduction of an ethnicity-targeted hierarchical α thalassaemia screening approach in this multi-ethnic Malaysian population would be effective. [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
26. Preliminary survey of ticks (Acari: Ixodidae) on cattle in northern Sudan
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Syahzuwan Hassan, A.M. El Hussein, Diaeldin A. Salih, and Frans Jongejan
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Male ,Veterinary medicine ,Ixodidae ,Climate ,Rhipicephalus sanguineus ,Population Dynamics ,Cattle Diseases ,Tick ,Hyalomma dromedarii ,Host-Parasite Interactions ,Sudan ,Species Specificity ,Abundance (ecology) ,Animals ,Acari ,Animal Husbandry ,lcsh:Veterinary medicine ,General Veterinary ,biology ,Outbreak ,General Medicine ,biology.organism_classification ,Tick Infestations ,Cross-Sectional Studies ,lcsh:SF600-1100 ,Cattle ,Female ,Seasons ,Amblyomma variegatum - Abstract
In a cross sectional survey conducted during the period June 2001 to July 2002, the geographical distribution of ticks on cattle in the Sudan was determined. Seventeen locations were surveyed from Northern, Central, Eastern, Western, Blue Nile and White Nile Provinces. Total body collections of ticks were made from 20 cattle at each location. Four tick genera and 11 species were identified. The tick species collected included Amblyomma lepidum, Amblyomma variegatum, Boophilus decoloratus, Hyalomma anatolicum anatolicum, Hyalomma dromedarii, Hyalomma impeltatum, Hyalomma marginatum rufipes, Hyalomma truncatum, Rhipicephalus evertsi evertsi, Rhipicephalus sanguineus group and Rhipicephalus simus simus. Major ecological changes have occurred due to extensive animal movement, deforestation, desertification and establishment of large mechanized agricultural schemes. These factors have certainly affected the distribution of ticks and tick-borne diseases in the Sudan. The absence of A. variegatum and A. lepidum in northern Sudan was not surprising, since these tick species are known to survive in humid areas and not in the desert and semi-desert areas of northern Sudan. The absence of B. annulatus in northern and central Sudan is in accordance with the finding that this tick species is restricted to the southern parts of the central Sudan. The presence of H. anatolicum anatolicum in Um Benin in relatively high abundance is an interesting finding. The present finding may indicate that the southern limit of this species has changed and moved southwards to latitude 13o N. It is concluded that major changes in tick distribution have taken place in the Sudan
27. An insight of-50 (G>A) mutation in the direct repeat element of the β-globin gene: From Malaysian perspective
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Mohd Yasin, N., Abdul Hamid, F. S., Syahzuwan Hassan, Mayusoff, Y., Mohd Sahid, E. N., and Esa, E.
28. Detection of β-globin gene mutations among β-thalassaemia carriers and patients in malaysia: Application of multiplex amplification refractory mutation system-polymerase chain reaction
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Syahzuwan Hassan, Ahmad, R., Zakaria, Z., Zulkafli, Z., and Abdullah, W. Z.
- Subjects
Original Article - Abstract
β-thalassaemia is one of the most common single-gene disorders worldwide. Each ethnic population has its own common mutations, accounting for the majority of cases, with a small number of mutations for the rarer alleles. Due to the heterogeneity of β-thalassaemia and the multi-ethnicity of Malaysians, molecular diagnostics may be expensive and time consuming.A simple polymerase chain reaction (PCR) approach involving a multiplex amplification refractory mutation system (MARMS) and one amplification refractory mutation system (ARMS), consisting of 20 β-globin gene mutations, were designed and employed to investigate β-thalassaemia patients and carriers.Out of 169 carriers tested with the MARMS, Cd 41/42 (-TTCT), Cd 26 (A-G) HbE, IVS 1-1 (G-T), and IVS 1-5 (G-C) were the most common mutations, accounting for 78.1%. Among the Malays, Cd 26 (A-G) HbE, Cd 41/42 (-TTCT), IVS 1-1 (G-T), and IVS 1-5 (G-C) were the most common mutations, accounting for 81.4%, whereas Cd 41/42 (-TTCT) and IVS 2-654 (C-T) were most common among the Chinese (79.1%).We propose the use of this cheap, easy to interpret, and simple system for the molecular diagnostics of β-thalassaemia among Malaysians at the Institute for Medical Research (IMR).
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