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39 results on '"Swiers, Gemma"'

1. A single mix gene in the axolotl - investigating mesendoderm and blood specification

Author

Swiers, Gemma

Subjects
571.862, Embryology, Germination
Abstract

All the tissues within the developing embryo are comprised of cells formed from one of the three germ layers defined during gastrulation; the ectoderm, mesoderm and endoderm. Understanding how the germ layers are formed and subsequently differentiate into different tissues is the basis of developmental biology.

Published
2008

5. 2018 – RECONSTRUCTION OF A DORSAL AORTA HEMOGENIC ENDOTHELIUM GENE REGULATORY NETWORK IDENTIFIES A RUNX1:IKZF1:NOTCH FEED-FORWARD LOOP

Author

Harman, Joe, primary, Greder, Lucas, additional, Swiers, Gemma, additional, Owens, Dominic, additional, Suciu, Maria, additional, Downes, Damien, additional, Telenius, Jelena, additional, Frontera, Vincent, additional, Antoniou, Stella, additional, Repapi, Emmanouela, additional, Azzoni, Emanuele, additional, and de Bruijn, Marella, additional

Published
2021
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6. A conserved mechanism for vertebrate mesoderm specification in urodele amphibians and mammals

Author

Swiers, Gemma, Chen, Yi-Hsien, Johnson, Andrew D., and Loose, Matthew

Subjects
Embryonic stem cells, Evolutionary biology, Anopheles, Biological sciences
Abstract

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.ydbio.2010.04.002 Byline: Gemma Swiers (a)(b), Yi-Hsien Chen (a), Andrew D. Johnson (a), Matthew Loose (a) Keywords: Mesoderm induction; Gene regulatory networks Abstract: Understanding how mesoderm is specified during development is a fundamental issue in biology, and it has been studied intensively in embryos from Xenopus. The gene regulatory network (GRN) for Xenopus is surprisingly complex and is not conserved in vertebrates, including mammals, which have single copies of the key genes Nodal and Mix. Why the Xenopus GRN should express multiple copies of Nodal and Mix genes is not known. To understand how these expanded gene families evolved, we investigated mesoderm specification in embryos from axolotls, representing urodele amphibians, since urodele embryology is basal to amphibians and was conserved during the evolution of amniotes, including mammals. We show that single copies of Nodal and Mix are required for mesoderm specification in axolotl embryos, suggesting the ancestral vertebrate state. Furthermore, we uncovered a novel genetic interaction in which Mix induces Brachyury expression, standing in contrast to the relationship of these molecules in Xenopus. However, we demonstrate that this functional relationship is conserved in mammals by showing that it is involved in the production of mesoderm from mouse embryonic stem cells. From our results, we produced an ancestral mesoderm (m)GRN, which we suggest is conserved in vertebrates. The results are discussed within the context of a theory in which the evolution of mechanisms governing early somatic development is constrained by the ancestral germ line-soma relationship, in which germ cells are produced by epigenesis. Author Affiliation: (a) Institute of Genetics, Queens Medical Centre, University of Nottingham, NG7 2UH, UK (b) Now at The Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, UK Article History: Received 7 December 2009; Revised 2 April 2010; Accepted 6 April 2010

Published
2010

7. Genetic regulatory networks programming hematopoietic stem cells and erythroid lineage specification

Author

Swiers, Gemma, Patient, Roger, and Loose, Matthew

Subjects
DNA binding proteins, Hematopoietic stem cells, Gene expression, Biological sciences
Abstract

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.ydbio.2006.02.051 Byline: Gemma Swiers (a), Roger Patient (b), Matthew Loose (a) Keywords: Stem cell; Transcriptional regulatory network; Network motif; Blood; Hematopoiesis; Erythroid lineage Abstract: Erythroid cell production results from passage through cellular hierarchies dependent on differential gene expression under the control of transcription factors responsive to changing niches. We have constructed Genetic Regulatory Networks (GRNs) describing this process, based predominantly on mouse data. Regulatory network motifs identified in E. coli and yeast GRNs are found in combination in these GRNs. Feed-forward motifs with autoregulation generate forward momentum and also control its rate, which is at its lowest in hematopoietic stem cells (HSCs). The simultaneous requirement for multiple regulators in multi-input motifs (MIMs) provides tight control over expression of target genes. Combinations of MIMs, exemplified by the SCL/LMO2 complexes, which have variable content and binding sites, explain how individual regulators can have different targets in HSCs and erythroid cells and possibly also how HSCs maintain stem cell functions while expressing lineage-affiliated genes at low level, so-called multi-lineage priming. MIMs combined with cross-antagonism describe the relationship between PU.1 and GATA-1 and between two of their target genes, Fli-1 and EKLF, with victory for GATA-1 and EKLF leading to erythroid lineage specification. These GRNs are useful repositories for current regulatory information, are accessible in interactive form via the internet, enable the consequences of perturbation to be predicted, and can act as seed networks to organize the rapidly accumulating microarray data. Author Affiliation: (a) Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK (b) The Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK Article History: Received 7 October 2005; Revised 24 February 2006; Accepted 28 February 2006

Published
2006

9. Hematopoietic Reprogramming In Vitro Informs In Vivo Identification of Hemogenic Precursors to Definitive Hematopoietic Stem Cells

Author

Pereira, Carlos-Filipe, primary, Chang, Betty, additional, Gomes, Andreia, additional, Bernitz, Jeffrey, additional, Papatsenko, Dmitri, additional, Niu, Xiaohong, additional, Swiers, Gemma, additional, Azzoni, Emanuele, additional, de Bruijn, Marella F.T.R., additional, Schaniel, Christoph, additional, Lemischka, Ihor R., additional, and Moore, Kateri A., additional

Published
2016
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10. Single-cell analyses of regulatory network perturbations using enhancer-targeting TALEs suggest novel roles for PU.1 during haematopoietic specification

Author

Wilkinson, Adam C., primary, Kawata, Viviane K. S., additional, Schütte, Judith, additional, Gao, Xuefei, additional, Antoniou, Stella, additional, Baumann, Claudia, additional, Woodhouse, Steven, additional, Hannah, Rebecca, additional, Tanaka, Yosuke, additional, Swiers, Gemma, additional, Moignard, Victoria, additional, Fisher, Jasmin, additional, Hidetoshi, Shimauchi, additional, Tijssen, Marloes R., additional, de Bruijn, Marella F. T. R., additional, Liu, Pentao, additional, and Göttgens, Berthold, additional

Published
2014
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14. Lymphomyeloid contribution of an immune-restricted progenitor emerging prior to definitive hematopoietic stem cells

Author

Böiers, C, Carrelha, J, Lutteropp, M, Luc, S, Green, J, Azzoni, E, Woll, P, Mead, A, Hultquist, A, Swiers, G, Perdiguero, E, Macaulay, I, Melchiori, L, Luis, T, Kharazi, S, Bouriez-Jones, T, Deng, Q, Pontén, A, Atkinson, D, Jensen, C, Sitnicka, E, Geissmann, F, Godin, I, Sandberg, R, de Bruijn, M, Jacobsen, S, Böiers, Charlotta, Carrelha, Joana, Lutteropp, Michael, Luc, Sidinh, Green, Joanna C A, Azzoni, Emanuele, Woll, Petter S, Mead, Adam J, Hultquist, Anne, Swiers, Gemma, Perdiguero, Elisa Gomez, Macaulay, Iain C, Melchiori, Luca, Luis, Tiago C, Kharazi, Shabnam, Bouriez-Jones, Tiphaine, Deng, Qiaolin, Pontén, Annica, Atkinson, Deborah, Jensen, Christina T, Sitnicka, Ewa, Geissmann, Frederic, Godin, Isabelle, Sandberg, Rickard, de Bruijn, Marella F T R, Jacobsen, Sten Eirik W, Böiers, C, Carrelha, J, Lutteropp, M, Luc, S, Green, J, Azzoni, E, Woll, P, Mead, A, Hultquist, A, Swiers, G, Perdiguero, E, Macaulay, I, Melchiori, L, Luis, T, Kharazi, S, Bouriez-Jones, T, Deng, Q, Pontén, A, Atkinson, D, Jensen, C, Sitnicka, E, Geissmann, F, Godin, I, Sandberg, R, de Bruijn, M, Jacobsen, S, Böiers, Charlotta, Carrelha, Joana, Lutteropp, Michael, Luc, Sidinh, Green, Joanna C A, Azzoni, Emanuele, Woll, Petter S, Mead, Adam J, Hultquist, Anne, Swiers, Gemma, Perdiguero, Elisa Gomez, Macaulay, Iain C, Melchiori, Luca, Luis, Tiago C, Kharazi, Shabnam, Bouriez-Jones, Tiphaine, Deng, Qiaolin, Pontén, Annica, Atkinson, Deborah, Jensen, Christina T, Sitnicka, Ewa, Geissmann, Frederic, Godin, Isabelle, Sandberg, Rickard, de Bruijn, Marella F T R, and Jacobsen, Sten Eirik W

Abstract

In jawed vertebrates, development of an adaptive immune-system is essential for protection of the born organism against otherwise life-threatening pathogens. Myeloid cells of the innate immune system are formed early in development, whereas lymphopoiesis has been suggested to initiate much later, following emergence of definitive hematopoietic stem cells (HSCs). Herein, we demonstrate that the embryonic lymphoid commitment process initiates earlier than previously appreciated, prior to emergence of definitive HSCs, through establishment of a previously unrecognized entirely immune-restricted and lymphoid-primed progenitor. Notably, this immune-restricted progenitor appears to first emerge in the yolk sac and contributes physiologically to the establishment of lymphoid and some myeloid components of the immune-system, establishing the lymphomyeloid lineage restriction process as an early and physiologically important lineage-commitment step in mammalian hematopoiesis

Published
2013

15. Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level

Author

Swiers, Gemma, primary, Baumann, Claudia, additional, O’Rourke, John, additional, Giannoulatou, Eleni, additional, Taylor, Stephen, additional, Joshi, Anagha, additional, Moignard, Victoria, additional, Pina, Cristina, additional, Bee, Thomas, additional, Kokkaliaris, Konstantinos D., additional, Yoshimoto, Momoko, additional, Yoder, Mervin C., additional, Frampton, Jon, additional, Schroeder, Timm, additional, Enver, Tariq, additional, Göttgens, Berthold, additional, and de Bruijn, Marella F. T. R., additional

Published
2013
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16. Lymphomyeloid Contribution of an Immune-Restricted Progenitor Emerging Prior to Definitive Hematopoietic Stem Cells

Author

Böiers, Charlotta, primary, Carrelha, Joana, additional, Lutteropp, Michael, additional, Luc, Sidinh, additional, Green, Joanna C.A., additional, Azzoni, Emanuele, additional, Woll, Petter S., additional, Mead, Adam J., additional, Hultquist, Anne, additional, Swiers, Gemma, additional, Perdiguero, Elisa Gomez, additional, Macaulay, Iain C., additional, Melchiori, Luca, additional, Luis, Tiago C., additional, Kharazi, Shabnam, additional, Bouriez-Jones, Tiphaine, additional, Deng, Qiaolin, additional, Pontén, Annica, additional, Atkinson, Deborah, additional, Jensen, Christina T., additional, Sitnicka, Ewa, additional, Geissmann, Frederic, additional, Godin, Isabelle, additional, Sandberg, Rickard, additional, de Bruijn, Marella F.T.R., additional, and Jacobsen, Sten Eirik W., additional

Published
2013
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18. Establishment of lympho-myeloid restricted progenitors prior to the emergence of definitive hematopoietic stem cells

Author

Böiers, Charlotta, primary, Lutteropp, Michael, additional, Luc, Sidinh, additional, Woll, Petter, additional, Mead, Adam, additional, Hultquist, Anne, additional, Carrelha, Joana, additional, Macaulay, Iain, additional, Swiers, Gemma, additional, Melchiori, Luca, additional, Luis, Tiago, additional, Kharazi, Shabnam, additional, Bouriez-Jones, Tiphaine, additional, Deng, Qiaolin, additional, Pontén, Annica, additional, Jensen, Christina, additional, Sitnicka, Ewa, additional, Sandberg, Rickard, additional, de Bruijn, Marella, additional, and Jacobsen, Sten Eirik, additional

Published
2013
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22. Erratum: Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis

Author

Moignard, Victoria, primary, Macaulay, Iain C., additional, Swiers, Gemma, additional, Buettner, Florian, additional, Schütte, Judith, additional, Calero-Nieto, Fernando J., additional, Kinston, Sarah, additional, Joshi, Anagha, additional, Hannah, Rebecca, additional, Theis, Fabian J., additional, Jacobsen, Sten Eirik, additional, de Bruijn, Marella F., additional, and Göttgens, Berthold, additional

Published
2013
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23. Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis

Author

Moignard, Victoria, primary, Macaulay, Iain C., additional, Swiers, Gemma, additional, Buettner, Florian, additional, Schütte, Judith, additional, Calero-Nieto, Fernando J., additional, Kinston, Sarah, additional, Joshi, Anagha, additional, Hannah, Rebecca, additional, Theis, Fabian J., additional, Jacobsen, Sten Eirik, additional, de Bruijn, Marella F., additional, and Göttgens, Berthold, additional

Published
2013
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39. Nonredundant roles for Runx1alternative promoters reflect their activity at discrete stages of developmental hematopoiesis

Author

Bee, Thomas, Swiers, Gemma, Muroi, Sawako, Pozner, Amir, Nottingham, Wade, Santos, Ana Cristina, Li, Pik-Shan, Taniuchi, Ichiro, and de Bruijn, Marella F.T.R

Abstract

The transcription factor Runx1 is a pivotal regulator of definitive hematopoiesis in mouse ontogeny. Vertebrate Runx1is transcribed from 2 promoters, the distal P1 and proximal P2, which provide a paradigm of the complex transcriptional and translational control of Runx1 function. However, very little is known about the biologic relevance of alternative Runx1promoter usage in definitive hematopoietic cell emergence. Here we report that both promoters are active at the very onset of definitive hematopoiesis, with a skewing toward the P2. Moreover, functional and morphologic analysis of a novel P1-null and an attenuated P2 mouse model revealed that although both promoters play important nonredundant roles in the emergence of definitive hematopoietic cells, the proximal P2 was most critically required for this. The nature of the observed phenotypes is indicative of a differential contribution of the P1 and P2 promoters to the control of overall Runx1 levels, where and when this is most critically required. In addition, the dynamic expression of P1-Runx1and P2-Runx1points at a requirement for Runx1 early in development, when the P2 is still the prevalent promoter in the emerging hemogenic endothelium and/or first committed hematopoietic cells.

Published
2010
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