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Key Message: Malt for craft "all-malt" brewing can have high quality, PHS resistance, and malted in normal timeframes. Canadian style adjunct malt is associated with PHS susceptibility. Expansion of malting barley production into non-traditional growing regions and erratic weather has increased the demand for preharvest sprouting (PHS) resistant, high quality malting barley cultivars. This is hindered by the relatively unknown relationships between PHS resistance and malting quality. Here we present a three-year study of malting quality and germination at different after-ripening durations post physiological maturity. Malting quality traits alpha amylase (AA) and free amino nitrogen (FAN) and germination rate at six days post PM shared a common association with a SNP in HvMKK3 on chromosome 5H in the Seed Dormancy 2 (SD2) region responsible for PHS susceptibility. Soluble protein (SP) and soluble over total protein (S/T) both shared a common association with a marker in the SD2 region. Significant genetic correlations between PHS resistance and the malting quality traits AA, FAN, SP, S/T were detected across and within HvMKK3 allele groups. High adjunct malt quality was related to PHS susceptibility. Selection for PHS resistance led to a correlated response in malting quality traits. Results strongly suggest pleiotropy of HvMKK3 on malting quality traits and that the classic "Canadian-style" malt is caused by a PHS susceptible allele of HvMKK3. PHS susceptibility appears to benefit the production of malt intended for adjunct brewing, while PHS resistance is compatible with all-malt brewing specifications. Here we present our analysis on the effect of combining complexly inherited and correlated traits with contrasting goals to inform breeding practice in malting barley, the general principles of which can be extended to other breeding programs., (© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)

Key Message: HvMKK3 alleles are temperature sensitive and are major contributors to environmental stability of preharvest sprouting in barley. Preharvest sprouting (PHS) can severely damage barley (Hordeum vulgare L.) malting quality, but PHS resistance is often negatively correlated with malting quality. Seed dormancy is closely related to PHS. Increased temperature during grain fill can decrease seed dormancy in barley, but genetic components of seed dormancy temperature sensitivity are poorly understood. Six years of PHS data were used to fit quantitative trait locus (QTL) x environment mixed models incorporating marker data from seed dormancy genes HvAlaAT1, HvGA20ox1, and HvMKK3 and weather covariates in spring and winter two-row malting barley. Variation in winter barley PHS was best modeled by average temperature range during grain fill and spring barley PHS by total precipitation during grain fill. Average high temperature during grain fill also accurately modeled PHS for both datasets. A highly non-dormant HvMKK3 allele determined baseline PHS susceptibility and HvAlaAT1 interactions with multiple HvMKK3 alleles conferred environmental sensitivity. Polygenic variation for PHS within haplotype was detected. Residual genotype and QTL by environment interaction variance indicated additional environmental and genetic factors involved in PHS. These models provide insight into genotype and environmental regulation of barley seed dormancy, a method for PHS forecasting, and a tool for breeders to improve PHS resistance., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

New breeding programs are faced with many challenges including evaluation of unknown germplasm, initiation of breeding populations that will satisfy short- and long-term breeding goals, and implementation of efficient phenotyping strategies for multiple traits. Genomic selection (GS) is a potentially valuable tool for recently established breeding programs to quickly accelerate genetic gain. Genomic selection on selection index (SI) values may increase gain over phenotypic selection but empirical studies remain limited. We compared gain in overall SI value for height, heading date, preharvest sprouting (PHS) resistance, and spot blotch resistance and component traits in two cycles of GS with one round of phenotypic selection (PS) in two-row spring malting barley (Hordeum vulgare L.). Higher realized gain for SI value, height, and PHS was observed with GS compared with PS but GS did not result in significant gain for heading date and spot blotch. Genetic variances for height and heading date, which had small index weights, were not reduced with GS but variances were substantially reduced for heavily weighted PHS and correlated seed germination traits. Inbreeding was increased by GS compared with PS but restricted mating of high breeding value individuals limited potential inbreeding. Our results indicate GS is a useful method to improve selection on index values with different weights., (© 2021 The Authors. The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America.)

Phosphorus (P) Index assessment requires independent estimates of long-term average annual P loss from fields, representing multiple climatic scenarios, management practices, and landscape positions. Because currently available measured data are insufficient to evaluate P Index performance, calibrated and validated process-based models have been proposed as tools to generate the required data. The objectives of this research were to develop a regional parameterization for the Agricultural Policy Environmental eXtender (APEX) model to estimate edge-of-field runoff, sediment, and P losses in restricted-layer soils of Missouri and Kansas and to assess the performance of this parameterization using monitoring data from multiple sites in this region. Five site-specific calibrated models (SSCM) from within the region were used to develop a regionally calibrated model (RCM), which was further calibrated and validated with measured data. Performance of the RCM was similar to that of the SSCMs for runoff simulation and had Nash-Sutcliffe efficiency (NSE) > 0.72 and absolute percent bias (|PBIAS|) < 18% for both calibration and validation. The RCM could not simulate sediment loss (NSE < 0, |PBIAS| > 90%) and was particularly ineffective at simulating sediment loss from locations with small sediment loads. The RCM had acceptable performance for simulation of total P loss (NSE > 0.74, |PBIAS| < 30%) but underperformed the SSCMs. Total P-loss estimates should be used with caution due to poor simulation of sediment loss. Although we did not attain our goal of a robust regional parameterization of APEX for estimating sediment and total P losses, runoff estimates with the RCM were acceptable for P Index evaluation., (Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.)

Process-based computer models have been proposed as a tool to generate data for Phosphorus (P) Index assessment and development. Although models are commonly used to simulate P loss from agriculture using managements that are different from the calibration data, this use of models has not been fully tested. The objective of this study is to determine if the Agricultural Policy Environmental eXtender (APEX) model can accurately simulate runoff, sediment, total P, and dissolved P loss from 0.4 to 1.5 ha of agricultural fields with managements that are different from the calibration data. The APEX model was calibrated with field-scale data from eight different managements at two locations (management-specific models). The calibrated models were then validated, either with the same management used for calibration or with different managements. Location models were also developed by calibrating APEX with data from all managements. The management-specific models resulted in satisfactory performance when used to simulate runoff, total P, and dissolved P within their respective systems, with > 0.50, Nash-Sutcliffe efficiency > 0.30, and percent bias within ±35% for runoff and ±70% for total and dissolved P. When applied outside the calibration management, the management-specific models only met the minimum performance criteria in one-third of the tests. The location models had better model performance when applied across all managements compared with management-specific models. Our results suggest that models only be applied within the managements used for calibration and that data be included from multiple management systems for calibration when using models to assess management effects on P loss or evaluate P Indices., (Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.)

The Agricultural Policy Environmental eXtender (APEX) model is capable of estimating edge-of-field water, nutrient, and sediment transport and is used to assess the environmental impacts of management practices. The current practice is to fully calibrate the model for each site simulation, a task that requires resources and data not always available. The objective of this study was to compare model performance for flow, sediment, and phosphorus transport under two parameterization schemes: a best professional judgment (BPJ) parameterization based on readily available data and a fully calibrated parameterization based on site-specific soil, weather, event flow, and water quality data. The analysis was conducted using 12 datasets at four locations representing poorly drained soils and row-crop production under different tillage systems. Model performance was based on the Nash-Sutcliffe efficiency (NSE), the coefficient of determination () and the regression slope between simulated and measured annualized loads across all site years. Although the BPJ model performance for flow was acceptable (NSE = 0.7) at the annual time step, calibration improved it (NSE = 0.9). Acceptable simulation of sediment and total phosphorus transport (NSE = 0.5 and 0.9, respectively) was obtained only after full calibration at each site. Given the unacceptable performance of the BPJ approach, uncalibrated use of APEX for planning or management purposes may be misleading. Model calibration with water quality data prior to using APEX for simulating sediment and total phosphorus loss is essential., (Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.)

The harmonic diffractive lens is a diffractive imaging lens for which the optical path-length transition between adjacent facets is an integer multiple m of the design wavelength λ(o). The total lens thickness in air is mλ(o)/(n - 1), which is m times thicker than the so-called modulo 2π diffractive lens. Lenses constructed in this way have hybrid properties of both refractive and diffractive lenses. Such a lens will have a diffraction-limited, common focus for a number of discrete wavelengths across the visible spectrum. A 34.75-diopter, 6-mm-diameter lens is diamond turned in aluminum and replicated in optical materials. The sag of the lens is 23 µm. Modulation transfer function measurements in both monochromatic and white light verify the performance of the lens. The lens approaches the diffraction limit for 10 discrete wavelengths across the visible spectrum.

Thermochromic materials can be used to record infrared holograms at 10.6 microm as has been demonstrated by others. The objective here was to determine quantitatively the capability of holographic recording techniques that utilize thermochromics. Particular emphasis is placed on cuprous mercuric iodide, but the procedure developed is valid for other thermochromic materials. The thermochromic Cu(2)HgI(4) mixed with a suitable binder and coated onto a temperature controlled substrate was used to record holograms at a wavelength of 10.6 microm. Thermal diffusivity and conductivity of the recording material (Cu(2)HgI(4) + binder) were measured and used to analyze the thermal behavior of the detector. Theory shows that a resolution of 50 l/mm can be attained. Experiments were conducted to demonstrate the theoretical predictions. Holograms were recorded and reconstructed at the wavelength of 10.6 microm.

A technique is proposed and demonstrated for measuring combustion temperatures using two-line laser-saturated fluorescence. The rotational temperature of OH is determined by saturating two different rotational transitions in the (0,0) band of the A(2)Sigma(+)-X(2)II electronic system and detecting fluorescence emission which originates from the laser-pumped upper rotational levels. Temperature is calculated from the ratio of the fluorescence intensities for the two different excitation-emission pairs. The method is demonstrated by measuring temperature profiles in subatmospheric H(2)/O(2)/Ar flat flames. Temperatures measured by two-line saturated fluorescence are compared with temperatures measured by coated thermocouples and OH absorption and with predictions from an elementary chemical kinetics code. The temperatures measured by the two-line fluorescence technique are accurate to 3-5% and exhibit low random error.

We report the first two-photon-excited hydrogen-atom fluorescence measurements in flames made to our knowledge. The n = 3 level of the H atom was excited by 205.1-nm radiation generated by Raman shifting a 224-nm beam produced by frequency mixing. Fluorescence was observed at 656.3 nm as a result of radiative decay from n = 3 to n = 2, the Balmer-alpha transition. A novel technique, photoionization-controlled loss spectroscopy, is proposed to eliminate the quenching dependence of the fluorescence signal.

The complexity of laser material processing can be greatly reduced using computer-generated phase reflection holographic scanners. These scanners direct and focus the beam of a carbon dioxide laser into a spot on the workpiece and then translate this spot over some general 2-D pattern as the scanner undergoes a simple 1-D motion. Procedures for constructing these scanners are presented, and the first-order aberrations introduced by them are analyzed. The primary aberrations cause the diffracted beam to focus to an astigmatic spot on the work surface. The severity of the astigmatism is proportional to the scan rate, scan angle, and f/number. A technique is presented in which the design of the scanner is adjusted so that the astigmatic image is aligned with the scan direction. The resolution perpendicular to the scan direction is the same as that of a scanner without aberrations of the same f/number. Materials processed using these scanners are presented to show their capabilities for carbon dioxide laser material processing. Power densities on the order of 10(6)/cm(2) can be readily obtained using the proposed technique.

The authors measured gamma-aminobutyric acid (GABA) levels in the lumbar CSF of patients with depression, with psychosis, or undergoing evaluation for a neurologic disorder. GABA levels in the CSF from depressed patients were significantly decreased compared with neurologic control patients. CSF GABA levels in psychotic patients were not different from those in neurologic patients, although the data suggested a decrease in CSF GABA levels in patients with schizoaffective disorder.

A single-pulse, laser-saturated fluorescence technique has been developed for absolute OH concentration measurements with a temporal resolution of 2 nsec, a spatial resolution of <0.1 mm(3), and an estimated accuracy of +/-30%. It has been applied in laminar, transitional, and turbulent hydrogen-air diffusion flames, providing the first reported quantitative measurements of average values, rms fluctuations, and probability density functions of OH radical concentration in nonpremixed flames.

This paper presents an analysis describing the thermal behavior of a thin film subjected to a spatially modulated irradiation field. The analysis shows that it is possible using a cw laser to establish a temperature field in such a film that maps the irradiation pattern with a resolution adequate for recording high quality holograms. Effects of the thermal properties of the film and substrate on such recording characteristics as linearity, sensitivity, and resolution are examined. A high diffraction efficiency recording technique based on the sublimation of a thin film situated on a constant temperature substrate is proposed as an example of the application of the analysis.

A time-resolved sequence of laser shadowgraph images is computer processed to produce 2-D optical flow maps showing the motion of the combustion interface between hot products and cold reactants. Background-subtracted shadowgraph images are reduced to skeletons, followed by local correlation between adjacent frames for computing optical flow. The resulting flow maps represent an initial step toward the goal of computer-assisted flowfield analysis and interpretation.

The balanced cross-rate model is proposed to analyze laser-induced molecular fluorescence signals when the laser pulse length is of the order of nanoseconds. Nanosecond pulse length lasers, specifically Q-switched Nd:YAG-pumped dye lasers, are attractive for saturated molecular fluorescence spectroscopy because of their high peak power and because their short pulse length minimizes the risk of laser-induced chemistry. In the balanced cross-rate model, single upper and lower rotational levels are assumed to be directly coupled by the laser radiation. Because the laser-induced processes which couple these levels are so fast at saturation intensities, a steady state is established between the two levels within picoseconds. Provided that the total population of the two laser-coupled rotational levels is constant during the laser pulse, the total molecular population can be calculated from the observed upper rotational level population using a two-level saturation model and Boltzmann statistics. Numerical simulation of the laser excitation dynamics of OH in an atmospheric pressure H(2)/O(2)/N(2) flame indicates that the balanced cross-rate model will give accurate results provided that the rotational relaxation rates in the upper and lower sets of rotational levels are approximately equal.

A procedure is presented for designing distortion-invariant correlation filters. Optical correlation filters designed using this technique retain full position invariance. The filter design begins by finding the distortion-invariant modes (eigenfunctions) for a particular image. The input image, filter, and correlation response are all spectrally expanded in terms of these orthogonal eigenfunctions. An iterative technique between the spatial domain and the spectral domain is used to rephase the invariant modes so that a filter composed of a linear combination of modes has the proper overall invariance. The iterative technique also controls the information content of the filter by maintaining specified amplitudes for each invariant mode in the filter. Targets are detected by spanning the filter to determine points of constant amplitude.

A new approach to the design of computer-generated holograms makes optimal use of the available device resolution. An iterative search algorithm minimizes an error criterion by directly manipulating the binary hologram and observing the effect on the desired reconstruction. Several measures of error and efficiency useful in assessing the optimality of digital holograms are defined. Methods for designing digital holograms that are based on projections and error diffusion are presented as established techniques for comparison to direct binary search.

A hybrid optical/electronic system performs median filtering and related ranked-order operations using threshold decomposition to encode the image. Threshold decomposition transforms the nonlinear neighborhood ranking operation into a linear space-invariant filtering step followed by a point-to-point threshold comparison step. Spatial multiplexing allows parallel processing of all the threshold components as well as recombination by a second linear space-invariant filtering step. An incoherent optical correlation system performs the linear filtering, using a magnetooptic spatial light modulator as the input device and a computergenerated hologram in the filter plane. Thresholding is done electronically. By adjusting the value of the threshold, the same architecture is used to perform median, minimum, and maximum filtering of images.

Iteratively designed filters are incorporated into an optical correlator for position, rotation, and intensity invariant recognition of target images. The filters exhibit excellent discrimination because they are designed to contain full information about the target image. Numerical simulations and experiments demonstrate detection of targets that are corrupted with random noise (SNR approximately 05) and also partially obscured by other objects. The complex valued filters are encoded in a computer generated hologram and fabricated directly using an electron-beam system. Experimental results using a liquid crystal spatial light modulator for realtime input show excellent agreement with analytical and numerical computations.

Laser speckle in holographic interferograms of three-dimensional transparent objects is eliminated at the expense of limiting the available viewing directions to a finite number. This is accomplished by using a phase grating to divide the object wave into a number of discrete plane waves propagating in various directions. Experimental results of the application of this technique to the study of natural convection above a heated wire are presented. Fringe resolution obtained by this technique is compared with that obtained using plane wave or diffuse illumination. A brief analysis of fringe localization in holographic interferometry is presented in the discussion.

Numerical and analytical techniques are presented that allow three-dimensional, asymmetric, refractive index fields to be reconstructed from optical pathlength measurements, which can be obtained using multidirectional holographic interferometry. Analytical reconstruction techniques that have been used in radioaptronomy and electron microscopy for a number of years, and recently in interferometry, are presented in the context of interferometric applications in the refractionless limit. These techniques require that optical pathlength data be collected over a 180 degrees angle of view. The required pathlength sampling rate is discussed. An efficient numerical procedure is developed for direct inversion of the data. Several numerical techniques are developed that do not require that data be collected over a full 180 degrees angle of view. All such techniques require redundant data to achieve accurate reconstructions. The required degree of redundancy increases as the angle of view decreases. Numerical simulations using six different reconstruction techniques indicate that with a 180 degrees angle of view, all are capable of providing accurate reconstructions. Four of the techniques were used to analyze simulated interferometric data recorded over an angle of view of less than 180 degrees . Examples of reasonably accurate reconstructions using data with angles of view as low as 45 degrees are presented.

A full-field, variable sensitivity technique for mapping the vibrational amplitude of a diffuse surface is demonstrated. The surface under study is placed in an optical field consisting of two mutually coherent plane waves which propagate in different angular directions. At any instant of time the interference of these waves causes a fringe pattern to be formed on the surface under study. The time average, over one or more vibrational periods, of the intensity pattern on the surface can be obtained in real time by utilizing visual persistance or by making a time-average recording of an image of the surface. The visibility of the fringe pattern thus obtained is found to be modulated by a function of the local amplitude of vibration. This modulation can be observed directly or a photograph of the pattern can be optically processed to yield fringes that are loci of constant vibrational amplitude.