Search

Your search keyword '"Swastik G. Pattanashetty"' showing total 6 results
Start Over Author "Swastik G. Pattanashetty"
6 results on '"Swastik G. Pattanashetty"'

1. Single-droplet surface-enhanced Raman scattering decodes the molecular determinants of liquid-liquid phase separation

Author

Anamika Avni, Ashish Joshi, Anuja Walimbe, Swastik G. Pattanashetty, and Samrat Mukhopadhyay

Subjects
Science
Abstract

The authors introduce a unique single-droplet surface-enhanced Raman scattering (SERS) methodology that illuminates a wealth of molecular information within the mesoscopic liquid condensed phase of Fused in Sarcoma in the absence and presence of RNA.

Published
2022
Full Text
View/download PDF

2. Contributors

Author

Iuliia A. Antifeeva, Hirofumi Arakawa, Graciela Lidia Boccaccio, Solomiia Boyko, Stefania Brocca, April L. Darling, Nathalie A. Djaja, Anna S. Fefilova, Ana Julia Fernández-Alvarez, Luisa A. Ferreira, Alexander V. Fonin, Laura R. Ganser, Jimena Giudice, Rita Grandori, Raza Haider, Helen Greenwood Hansma, Nikolay Ilyinsky, Pavel Ivanov, Brett Janis, Hao Jiang, Ashish Joshi, Irina M. Kuznetsova, David S. Libich, Sonia Longhi, Michael A. Menze, Yakov I. Mokin, Samrat Mukhopadhyay, Sua Myong, Niharika Nag, Semen Nesterov, Woei Shyuan Ng, Nobuo N. Noda, George L. Parra, Sunita Pathak, Swastik G. Pattanashetty, Giulia Pesce, Andrea Putnam, Claire L. Riggs, Santanu Sasidharan, Prakash Saudagar, Geraldine Seydoux, Hendrik Sielaff, Sergey A. Silonov, Evan Spruijt, Lucia C. Strader, Witold K. Surewicz, María Gabriela Thomas, P. Todd Stukenberg, Timir Tripathi, Konstantin K. Turoverov, Vladimir N. Uversky, Anuja Walimbe, Boris Y. Zaslavsky, and Ziqing Winston Zhao

Published
2023
Full Text
View/download PDF

4. Single-Droplet Surface-Enhanced Raman Scattering Decodes the Molecular Language of Liquid-Liquid Phase Separation

Author

Anamika Avni, Ashish Joshi, Anuja Walimbe, Swastik G. Pattanashetty, and Samrat Mukhopadhyay

Subjects
technology, industry, and agriculture
Abstract

Biomolecular condensates formed via liquid-liquid phase separation (LLPS) are involved in a myriad of critical cellular functions and debilitating neurodegenerative diseases. Elucidating the role of intrinsic disorder and conformational heterogeneity of intrinsically disordered proteins/regions (IDPs/IDRs) in these phase-separated membrane-less organelles is crucial to understanding the mechanism of formation and regulation of biomolecular condensates. Here we introduce a unique single-droplet surface-enhanced Raman scattering (SERS) methodology that utilizes surface-engineered, plasmonic, metal nanoparticles to unveil the inner workings of mesoscopic liquid droplets of Fused in Sarcoma (FUS) in the absence and presence of RNA. These highly sensitive measurements offer unprecedented sensitivity to capture the crucial interactions, conformational heterogeneity, and structural distributions within the condensed phase in a droplet-by-droplet manner. Such an ultra-sensitive single-droplet vibrational methodology can serve as a potent tool to decipher the key molecular drivers of biological phase transitions of a wide range of biomolecular condensates involved in physiology and disease.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results